Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0010153

Physical Location & Seq

Genomic Coordinates Forward (+)

39144099 .. 39162021

Gene Structure 17923 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1278700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1890 bp

ATGGAAATGGAAATAGACCCCGATGACCTTTTCAGAGACGACGACGACGACGCAGACAATGAACTCTTCCAGGGAAGGGAGTCGACGAAGGATCTTGTGGTGTACTTGGTTGATGCTTCCCCCAAAATGTTTAGCACTACTTGCCCATCCGGGGACGATAAGGACGAAACTCATTTTCATGTTGCTGTGAGTTGTATCGCACAGTCTCTGAAGACACAAATCATTAATCATGCATATGATGAAGTTGCCATATGCTTCTTTAACACTAGGGAAAAATGGAACTTGCAAGATTTAAATGGTGTTTATGTGTTTAACGTTGCTGAACAAGAGTATCTAGACAGGCCAACAGCAAGGCTAATTAAAGAAATTGATAGCGTAGAAGAATCATTTATGAGCAAAATCGGGAGTCAGTATGGTATTGTCTCTGGGTCCCGAGAAAATTCTCTTTACAATGCCCTCTGGGTGGCCCAAGCACTGTTGCGTAAAGGATCTTCGAAGACAGCCGAAAAGCGCGTTCTTTTATTTACAAATGAAGACAATCCATTTGGCAACATTACGGGAGTGATAAAGACAGATATGATGAGGACCACATTGCAGCGGGCTAGAGATGCACAAGATCTTGGCATCTCAATTGAACTTCTTCCATTGAGTCGACCTGACAGTGATTTCAATGTTTCTACTTTCTACTCTGATTTACTTGGACTCAAAAGTGATGATCTTGCTCGATTTATGCCCGCAGCAGGAAAAAAATTGGAAGATATGAAGGATCAGCTTAGAAAGAAAATGTTCAAGAAGCGCCTAGTTAGAAAAATCACTTTCTCAATTGCCAATGGATTATCAATTCAACTGAATTCATATGCTTTAATTCGGCCCACTCTTCCAGGAGCGATAACCTGGCTTGATTCTGTGACTAATCGTCCTCTGAAGGCTGAAAGATCTTTCATCTGTGCTGATACTGGGGCATTGGTGCAAGAATCTGCTAAACGTTTTCAACCTTACAAGAGTGAAAATATAAAGTTTTCGGTGGAGGAGCTTTCGGAGATAAAAAGATTTTCAGCTGGACATCTTCGTCTTCTTGGTTTCAAGCCATTAAATTGCTTAAAAGAGTTTCACAACTTGAGGCCGTCAACATTTGTTTTTCCTACTGATGAGGAACTGATTGGTAGCACATCTATTTTCATTGCCCTTCATAGATCCATGCTAAGGCTAAACCGTTTTGCAGTTGCATTTTATGGGAGTTCATCTCGCCCACAATTGGTTGCTCTTGTTGCCCAAGATGAAATAATTAGTGCTGGTGGTCAGGTTGAACCACCGGGAATGCATATGATATATCTTCCGTACTCCGAGGACATTAGAAACACTGAAGAGCTTCATACAGGTTCAAATGATGCACCACCTCATGCAAATGATGATCAAATCAGAAAAGCTGCTGCTTTAATCAAACGTTTTGACTTGAAAGATTTCTCAGTATTCCAATTTGCCAATCCTGCCTTGCAGAGACACTATGCAGTACTGCAGGCCCTAGCGCTGGAGGAAGAAGAAATACCAGAAACCCAGGATGAAACAGTGCCTGATGAGGAAGGAATGTCTAGACCCACATTTGTTAGTGCATTAGAAGAATTTAAGCTGTCTGTCTATGGGGACAATTGTGACGAGGAAAATGCAGTGGGAAATGGAAAAGAAAGTGAGACCTCCAAAAAACGAAAAGCTGTTGCTGACAATGCAGTCAAAGAGTGTGCTAACTATGATTGGGGTGGCCTTGCAGACAACGGAAAGTTAAAGGATATGACAGTGACACAGTTGAAGTACTATCTGACGGCAAATAATCTTCCGCTTGCTGGGAAGAAGGAAGCTTTGATTAGTCGAATTTTGACACACTTGGGGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000723 GO:0000726 GO:0000781 GO:0000784 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009628 GO:0009987 GO:0016043 GO:0031974 GO:0031981 GO:0032200 GO:0033554 GO:0034641 GO:0042162 GO:0042592 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043565 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0060249 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0098687 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03450 map03450

Pfam Domains

Protein Families

Ku Ku_C Ku_N SAP

Protein Analysis

630

Amino Acids

70.71

Weight (kDa)

5.27

Isoelectric Point (pI)

46.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ku_N	PF03731	33 - 266	4.5e-54	Ku70/Ku80 N-terminal alpha/beta domain
Ku	PF02735	276 - 472	1.6e-35	Ku70/Ku80 beta-barrel domain
Ku_C	PF03730	488 - 569	1.1e-17	Ku70/Ku80 C-terminal arm
SAP	PF02037	593 - 627	1.1e-07	SAP domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010153)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G16970
fragaria_vesca	FvH4_4g12150 FvH4_4g12150 FvH4_4g12150 FvH4_4g12150
malus_domestica	MD13G1278700.v1.1
prunus_persica	Prupe.1G178000_v2.0.a1 Prupe.1G178000_v2.0.a1
pyrus_communis	pycom13g29490
rosa_chinensis	RchiOBHm_Chr4g0409801
rosa_laevigata	RLG00000008521
rosa_multiflora	Rmu_sc0019800.1_g000001 Rmu_sc0042987.1_g000001
rosa_roxburghii	Rroxscaffold_5G00354350
rosa_rugosa	Rorug04G0096600
rosa_samantha	Rh4AG158200 Rh4BG157900 Rh4CG168700 Rh4DG152800
rosa_wichuraiana	Rw0G017490 Rw4G013070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1068, 1724
AccI	GTMKAC	2 cut(s)	83, 652
AccII	CGCG	1 cut(s)	513
AciI	CCGC	3 cut(s)	598, 735, 1832
AclI	AACGTT	3 cut(s)	315, 985, 1444
AclWI	GGATC	4 cut(s)	99, 496, 774, 1188
AcsI	RAATTY	4 cut(s)	439, 850, 1619, 1866
AcuI	CTGAAG	3 cut(s)	230, 944, 1383
AfaI	GTAC	4 cut(s)	104, 1340, 1512, 1808
AfeI	AGCGCT	1 cut(s)	1527
AfiI	CCNNNNNNNGG	8 cut(s)	76, 151, 463, 740, 1203, 1255, 1528, 1838
AhdI	GACNNNNNGTC	1 cut(s)	915
AjnI	CCWGG	4 cut(s)	69, 880, 893, 1554
AluBI	AGCT	8 cut(s)	772, 1033, 1058, 1369, 1427, 1627, 1709, 1853
AluI	AGCT	8 cut(s)	772, 1033, 1058, 1369, 1427, 1627, 1709, 1853
Alw26I	GTCTC	5 cut(s)	30, 210, 427, 1492, 1682
AlwI	GGATC	4 cut(s)	99, 496, 774, 1188
AlwNI	CAGNNNCTG	2 cut(s)	208, 1571
Ama87I	CYCGRG	1 cut(s)	432
Aor51HI	AGCGCT	1 cut(s)	1527
AoxI	GGCC	6 cut(s)	341, 465, 869, 1121, 1518, 1756
ApeKI	GCWGC	4 cut(s)	595, 737, 1427, 1430
ApoI	RAATTY	4 cut(s)	439, 850, 1619, 1866
ArsI	GACNNNNNNTTYG	2 cut(s)	527, 559
AseI	ATTAAT	1 cut(s)	225
Asp700I	GAANNNNTTC	4 cut(s)	639, 785, 1368, 1460
AspLEI	GCGC	3 cut(s)	513, 798, 1528
AspS9I	GGNCC	5 cut(s)	429, 466, 585, 870, 1519
AsuC2I	CCSGG	2 cut(s)	151, 1314
AsuII	TTCGAA	1 cut(s)	494
AvaI	CYCGRG	1 cut(s)	432
AvaII	GGWCC	2 cut(s)	429, 585
BaeI	ACNNNNGTAYC	2 cut(s)	314, 347
BarI	GAAGNNNNNNTAC	4 cut(s)	475, 507, 1527, 1559
BbsI	GAAGAC	4 cut(s)	218, 503, 540, 1064
BbvI	GCAGC	4 cut(s)	607, 749, 1414, 1417
BccI	CCATC	1 cut(s)	154
BceAI	ACGGC	2 cut(s)	1108, 1833
BcgI	CGANNNNNNTGC	2 cut(s)	1643, 1677
BciT130I	CCWGG	4 cut(s)	71, 882, 895, 1556
BclI	TGATCA	1 cut(s)	1411
BcnI	CCSGG	2 cut(s)	151, 1314
BcoDI	GTCTC	5 cut(s)	30, 210, 427, 1492, 1682
BfaI	CTAG	6 cut(s)	267, 335, 603, 800, 1523, 1590
BfmI	CTRYAG	1 cut(s)	1514
BfoI	RGCGCY	2 cut(s)	799, 1529
BglII	AGATCT	2 cut(s)	616, 935
BisI	GCNGC	4 cut(s)	596, 738, 1428, 1431
BlsI	GCNGC	4 cut(s)	597, 739, 1429, 1432
BmcAI	AGTACT	2 cut(s)	1512, 1808
Bme1390I	CCNGG	6 cut(s)	71, 151, 882, 895, 1314, 1556
Bme18I	GGWCC	2 cut(s)	429, 585
BmeRI	GACNNNNNGTC	1 cut(s)	915
BmeT110I	CYCGRG	1 cut(s)	432
BmgT120I	GGNCC	5 cut(s)	429, 466, 585, 870, 1519
BmiI	GGNNCC	2 cut(s)	430, 431
BmrFI	CCNGG	6 cut(s)	71, 151, 882, 895, 1314, 1556
BmrI	ACTGGG	1 cut(s)	966
BmsI	GCATC	4 cut(s)	103, 598, 633, 1378
BmuI	ACTGGG	1 cut(s)	966
BpiI	GAAGAC	4 cut(s)	218, 503, 540, 1064
BplI	GAGNNNNNCTC	2 cut(s)	1228, 1260
BpmI	CTGGAG	1 cut(s)	1550
Bpu10I	CCTNAGC	1 cut(s)	1202
Bpu14I	TTCGAA	1 cut(s)	494
BpuEI	CTTGAG	1 cut(s)	1138
BpuMI	CCSGG	2 cut(s)	151, 1314
BsaI	GGTCTC	1 cut(s)	1682
BsaJI	CCNNGG	4 cut(s)	70, 150, 1344, 1554
Bsc4I	CCNNNNNNNGG	8 cut(s)	76, 151, 463, 740, 1203, 1255, 1528, 1838
Bse1I	ACTGG	1 cut(s)	961
Bse3DI	GCAATG	2 cut(s)	590, 1179
BseBI	CCWGG	4 cut(s)	71, 882, 895, 1556
BseDI	CCNNGG	4 cut(s)	70, 150, 1344, 1554
BseGI	GGATG	2 cut(s)	146, 1564
BseLI	CCNNNNNNNGG	8 cut(s)	76, 151, 463, 740, 1203, 1255, 1528, 1838
BseMI	GCAATG	2 cut(s)	590, 1179
BseMII	CTCAG	1 cut(s)	1479
BseNI	ACTGG	1 cut(s)	961
BseRI	GAGGAG	1 cut(s)	1043
BseXI	GCAGC	4 cut(s)	607, 749, 1414, 1417
BseYI	CCCAGC	1 cut(s)	1838
Bsh1236I	CGCG	1 cut(s)	513
BshFI	GGCC	6 cut(s)	343, 467, 871, 1123, 1520, 1758
BsiHKCI	CYCGRG	1 cut(s)	432
BsiSI	CCGG	2 cut(s)	150, 1313
BslFI	GGGAC	3 cut(s)	167, 415, 1655
BslI	CCNNNNNNNGG	8 cut(s)	76, 151, 463, 740, 1203, 1255, 1528, 1838
BsmAI	GTCTC	5 cut(s)	30, 210, 427, 1492, 1682
BsmBI	CGTCTC	1 cut(s)	30
BsmFI	GGGAC	3 cut(s)	167, 415, 1655
BsmI	GAATGC	1 cut(s)	1323
BsnI	GGCC	6 cut(s)	343, 467, 871, 1123, 1520, 1758
Bso31I	GGTCTC	1 cut(s)	1682
BsoBI	CYCGRG	1 cut(s)	432
Bsp119I	TTCGAA	1 cut(s)	494
Bsp143I	GATC	8 cut(s)	91, 488, 616, 715, 766, 935, 1193, 1411
BspACI	CCGC	3 cut(s)	598, 735, 1832
BspANI	GGCC	6 cut(s)	343, 467, 871, 1123, 1520, 1758
BspCNI	CTCAG	1 cut(s)	1478
BspFNI	CGCG	1 cut(s)	513
BspLI	GGNNCC	2 cut(s)	430, 431
BspMAI	CTGCAG	1 cut(s)	1518
BspPI	GGATC	4 cut(s)	99, 496, 774, 1188
BspQI	GCTCTTC	1 cut(s)	1359
BspT104I	TTCGAA	1 cut(s)	494
BspTNI	GGTCTC	1 cut(s)	1682
BsrDI	GCAATG	2 cut(s)	590, 1179
BsrI	ACTGG	1 cut(s)	961
BssECI	CCNNGG	4 cut(s)	70, 150, 1344, 1554
BssMI	GATC	8 cut(s)	91, 488, 616, 715, 766, 935, 1193, 1411
Bst2UI	CCWGG	4 cut(s)	71, 882, 895, 1556
Bst4CI	ACNGT	7 cut(s)	204, 477, 662, 1214, 1567, 1792, 1800
Bst6I	CTCTTC	3 cut(s)	71, 882, 1359
BstAPI	GCANNNNNTGC	1 cut(s)	141
BstBI	TTCGAA	1 cut(s)	494
BstC8I	GCNNGC	4 cut(s)	600, 735, 1518, 1836
BstDEI	CTNAG	3 cut(s)	773, 1202, 1465
BstF5I	GGATG	2 cut(s)	146, 1564
BstFNI	CGCG	1 cut(s)	513
BstH2I	RGCGCY	2 cut(s)	799, 1529
BstHHI	GCGC	3 cut(s)	513, 798, 1528
BstKTI	GATC	8 cut(s)	94, 491, 619, 718, 769, 938, 1196, 1414
BstMAI	GTCTC	5 cut(s)	30, 210, 427, 1492, 1682
BstMBI	GATC	8 cut(s)	91, 488, 616, 715, 766, 935, 1193, 1411
BstMWI	GCNNNNNNNGC	5 cut(s)	141, 608, 1268, 1487, 1721
BstNI	CCWGG	4 cut(s)	71, 882, 895, 1556
BstSCI	CCNGG	6 cut(s)	69, 149, 880, 893, 1312, 1554
BstSFI	CTRYAG	1 cut(s)	1514
BstUI	CGCG	1 cut(s)	513
BstV1I	GCAGC	4 cut(s)	607, 749, 1414, 1417
BstV2I	GAAGAC	4 cut(s)	218, 503, 540, 1064
BstX2I	RGATCY	5 cut(s)	91, 488, 616, 935, 1193
BstYI	RGATCY	5 cut(s)	91, 488, 616, 935, 1193
BsuRI	GGCC	6 cut(s)	343, 467, 871, 1123, 1520, 1758
BtsCI	GGATG	2 cut(s)	146, 1564
BtsI	GCAGTG	1 cut(s)	1671
BtsIMutI	CAGTG	6 cut(s)	473, 667, 1359, 1572, 1671, 1797
Cac8I	GCNNGC	4 cut(s)	600, 735, 1518, 1836
CaiI	CAGNNNCTG	2 cut(s)	208, 1571
CfoI	GCGC	3 cut(s)	513, 798, 1528
Cfr13I	GGNCC	5 cut(s)	429, 466, 585, 870, 1519
CseI	GACGC	1 cut(s)	59
Csp6I	GTAC	4 cut(s)	103, 1339, 1511, 1807
CviAII	CATG	4 cut(s)	179, 230, 1198, 1400
CviQI	GTAC	4 cut(s)	103, 1339, 1511, 1807
DdeI	CTNAG	3 cut(s)	773, 1202, 1465
DpnI	GATC	8 cut(s)	93, 490, 618, 717, 768, 937, 1195, 1413
DpnII	GATC	8 cut(s)	91, 488, 616, 715, 766, 935, 1193, 1411
DraI	TTTAAA	1 cut(s)	294
DrdI	GACNNNNNNGTC	2 cut(s)	1068, 1724
DriI	GACNNNNNGTC	1 cut(s)	915
DseDI	GACNNNNNNGTC	2 cut(s)	1068, 1724
Eam1104I	CTCTTC	3 cut(s)	71, 882, 1359
Eam1105I	GACNNNNNGTC	1 cut(s)	915
EarI	CTCTTC	3 cut(s)	71, 882, 1359
Eco31I	GGTCTC	1 cut(s)	1682
Eco47I	GGWCC	2 cut(s)	429, 585
Eco47III	AGCGCT	1 cut(s)	1527
Eco57I	CTGAAG	3 cut(s)	230, 944, 1383
Eco88I	CYCGRG	1 cut(s)	432
EcoO109I	RGGNCCY	2 cut(s)	429, 1519
EcoRI	GAATTC	1 cut(s)	850
EcoRII	CCWGG	4 cut(s)	69, 880, 893, 1554
EcoT22I	ATGCAT	2 cut(s)	235, 1323
Esp3I	CGTCTC	1 cut(s)	30
FaeI	CATG	4 cut(s)	182, 233, 1201, 1403
FaqI	GGGAC	3 cut(s)	167, 415, 1655
FatI	CATG	4 cut(s)	178, 229, 1197, 1399
FauI	CCCGC	2 cut(s)	591, 742
FauNDI	CATATG	4 cut(s)	235, 251, 856, 1323
FbaI	TGATCA	1 cut(s)	1411
FblI	GTMKAC	2 cut(s)	83, 652
Fnu4HI	GCNGC	4 cut(s)	596, 738, 1428, 1431
FokI	GGATG	2 cut(s)	133, 1571
Fsp4HI	GCNGC	4 cut(s)	596, 738, 1428, 1431
FspBI	CTAG	6 cut(s)	267, 335, 603, 800, 1523, 1590
GlaI	GCGC	3 cut(s)	512, 797, 1527
GluI	GCNGC	4 cut(s)	596, 738, 1428, 1431
GsaI	CCCAGC	1 cut(s)	1842
GsuI	CTGGAG	1 cut(s)	1550
HaeII	RGCGCY	2 cut(s)	799, 1529
HaeIII	GGCC	6 cut(s)	343, 467, 871, 1123, 1520, 1758
HapII	CCGG	2 cut(s)	150, 1313
HgaI	GACGC	1 cut(s)	59
HhaI	GCGC	3 cut(s)	513, 798, 1528
Hin1II	CATG	4 cut(s)	182, 233, 1201, 1403
Hin6I	GCGC	3 cut(s)	511, 796, 1526
HinP1I	GCGC	3 cut(s)	511, 796, 1526
HincII	GTYRAC	3 cut(s)	84, 653, 1128
HindII	GTYRAC	3 cut(s)	84, 653, 1128
HindIII	AAGCTT	1 cut(s)	1851
HinfI	GANTC	7 cut(s)	80, 383, 406, 649, 702, 902, 974
HpaII	CCGG	2 cut(s)	150, 1313
Hpy166II	GTNNAC	4 cut(s)	84, 103, 653, 1128
Hpy188I	TCNGA	8 cut(s)	35, 210, 691, 924, 1039, 1345, 1421, 1815
Hpy188III	TCNNGA	5 cut(s)	335, 403, 432, 790, 1590
Hpy8I	GTNNAC	4 cut(s)	84, 103, 653, 1128
Hpy99I	CGWCG	5 cut(s)	44, 47, 50, 53, 88
HpyAV	CCTTC	7 cut(s)	69, 82, 757, 919, 1196, 1574, 1840
HpyCH4III	ACNGT	7 cut(s)	204, 477, 662, 1214, 1567, 1792, 1800
HpyCH4IV	ACGT	3 cut(s)	315, 985, 1444
HpyF10VI	GCNNNNNNNGC	5 cut(s)	141, 608, 1268, 1487, 1721
HpyF3I	CTNAG	3 cut(s)	773, 1202, 1465
HpySE526I	ACGT	3 cut(s)	315, 985, 1444
Hsp92II	CATG	4 cut(s)	182, 233, 1201, 1403
HspAI	GCGC	3 cut(s)	511, 796, 1526
KflI	GGGWCCC	1 cut(s)	429
Ksp22I	TGATCA	1 cut(s)	1411
Kzo9I	GATC	8 cut(s)	91, 488, 616, 715, 766, 935, 1193, 1411
LguI	GCTCTTC	1 cut(s)	1359
LmnI	GCTCC	2 cut(s)	884, 1030
Lsp1109I	GCAGC	4 cut(s)	607, 749, 1414, 1417
LweI	GCATC	4 cut(s)	103, 598, 633, 1378
MaeI	CTAG	6 cut(s)	267, 335, 603, 800, 1523, 1590
MaeII	ACGT	3 cut(s)	315, 985, 1444
MaeIII	GTNAC	3 cut(s)	907, 1649, 1792
MalI	GATC	8 cut(s)	93, 490, 618, 717, 768, 937, 1195, 1413
MboI	GATC	8 cut(s)	91, 488, 616, 715, 766, 935, 1193, 1411
MfeI	CAATTG	4 cut(s)	630, 822, 1253, 1645
MflI	RGATCY	5 cut(s)	91, 488, 616, 935, 1193
MlyI	GAGTC	4 cut(s)	89, 415, 658, 696
Mph1103I	ATGCAT	2 cut(s)	235, 1323
MroXI	GAANNNNTTC	4 cut(s)	639, 785, 1368, 1460
MslI	CAYNNNNRTG	3 cut(s)	177, 234, 1404
MspA1I	CMGCKG	2 cut(s)	598, 1058
MspI	CCGG	2 cut(s)	150, 1313
MspR9I	CCNGG	6 cut(s)	71, 151, 882, 895, 1314, 1556
MunI	CAATTG	4 cut(s)	630, 822, 1253, 1645
Mva1269I	GAATGC	1 cut(s)	1323
MvaI	CCWGG	4 cut(s)	71, 882, 895, 1556
MvnI	CGCG	1 cut(s)	513
MwoI	GCNNNNNNNGC	5 cut(s)	141, 608, 1268, 1487, 1721
NciI	CCSGG	2 cut(s)	151, 1314
NdeI	CATATG	4 cut(s)	235, 251, 856, 1323
NdeII	GATC	8 cut(s)	91, 488, 616, 715, 766, 935, 1193, 1411
NlaIII	CATG	4 cut(s)	182, 233, 1201, 1403
NlaIV	GGNNCC	2 cut(s)	430, 431
NmuCI	GTSAC	3 cut(s)	907, 1649, 1792
NsiI	ATGCAT	2 cut(s)	235, 1323
NspV	TTCGAA	1 cut(s)	494
PciSI	GCTCTTC	1 cut(s)	1359
PcsI	WCGNNNNNNNCGW	2 cut(s)	45, 162
PctI	GAATGC	1 cut(s)	1323
PdmI	GAANNNNTTC	4 cut(s)	639, 785, 1368, 1460
PfeI	GAWTC	3 cut(s)	383, 902, 974
PfoI	TCCNGGA	1 cut(s)	880
PkrI	GCNGC	4 cut(s)	597, 739, 1429, 1432
PleI	GAGTC	4 cut(s)	88, 414, 657, 696
PpsI	GAGTC	4 cut(s)	88, 414, 657, 696
PpuMI	RGGWCCY	1 cut(s)	429
PshBI	ATTAAT	1 cut(s)	225
Psp1406I	AACGTT	3 cut(s)	315, 985, 1444
Psp5II	RGGWCCY	1 cut(s)	429
Psp6I	CCWGG	4 cut(s)	69, 880, 893, 1554
PspFI	CCCAGC	1 cut(s)	1838
PspGI	CCWGG	4 cut(s)	69, 880, 893, 1554
PspN4I	GGNNCC	2 cut(s)	430, 431
PspPI	GGNCC	5 cut(s)	429, 466, 585, 870, 1519
PspPPI	RGGWCCY	1 cut(s)	429
PstI	CTGCAG	1 cut(s)	1518
PstNI	CAGNNNCTG	2 cut(s)	208, 1571
PsuI	RGATCY	5 cut(s)	91, 488, 616, 935, 1193
PvuII	CAGCTG	1 cut(s)	1058
RsaI	GTAC	4 cut(s)	104, 1340, 1512, 1808
RsaNI	GTAC	4 cut(s)	103, 1339, 1511, 1807
RseI	CAYNNNNRTG	3 cut(s)	177, 234, 1404
SalI	GTCGAC	2 cut(s)	82, 651
SapI	GCTCTTC	1 cut(s)	1359
SatI	GCNGC	4 cut(s)	596, 738, 1428, 1431
Sau3AI	GATC	8 cut(s)	91, 488, 616, 715, 766, 935, 1193, 1411
Sau96I	GGNCC	5 cut(s)	429, 466, 585, 870, 1519
ScaI	AGTACT	2 cut(s)	1512, 1808
SchI	GAGTC	4 cut(s)	89, 415, 658, 696
ScrFI	CCNGG	6 cut(s)	71, 151, 882, 895, 1314, 1556
SfaNI	GCATC	4 cut(s)	103, 598, 633, 1378
SfcI	CTRYAG	1 cut(s)	1514
SfuI	TTCGAA	1 cut(s)	494
SinI	GGWCC	2 cut(s)	429, 585
SmiI	ATTTAAAT	1 cut(s)	294
SmiMI	CAYNNNNRTG	3 cut(s)	177, 234, 1404
SmlI	CTYRAG	1 cut(s)	1117
SmoI	CTYRAG	1 cut(s)	1117
SsiI	CCGC	3 cut(s)	598, 735, 1832
SspMI	CTAG	6 cut(s)	267, 335, 603, 800, 1523, 1590
StyD4I	CCNGG	6 cut(s)	69, 149, 880, 893, 1312, 1554
SwaI	ATTTAAAT	1 cut(s)	294
TaaI	ACNGT	7 cut(s)	204, 477, 662, 1214, 1567, 1792, 1800
TaiI	ACGT	3 cut(s)	318, 988, 1447
TaqI	TCGA	5 cut(s)	83, 494, 652, 724, 1864
TatI	WGTACW	3 cut(s)	102, 1510, 1806
TfiI	GAWTC	3 cut(s)	383, 902, 974
TscAI	CASTG	6 cut(s)	480, 667, 1366, 1572, 1671, 1797
TseFI	GTSAC	3 cut(s)	907, 1649, 1792
TseI	GCWGC	4 cut(s)	595, 737, 1427, 1430
Tsp45I	GTSAC	3 cut(s)	907, 1649, 1792
TspGWI	ACGGA	2 cut(s)	1326, 1785
TspRI	CASTG	6 cut(s)	480, 667, 1366, 1572, 1671, 1797
VpaK11BI	GGWCC	2 cut(s)	429, 585
VspI	ATTAAT	1 cut(s)	225
XapI	RAATTY	4 cut(s)	439, 850, 1619, 1866
XbaI	TCTAGA	2 cut(s)	334, 1589
XmiI	GTMKAC	2 cut(s)	83, 652
XmnI	GAANNNNTTC	4 cut(s)	639, 785, 1368, 1460
XspI	CTAG	6 cut(s)	267, 335, 603, 800, 1523, 1590
ZrmI	AGTACT	2 cut(s)	1512, 1808
Zsp2I	ATGCAT	2 cut(s)	235, 1323

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.