Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004704

Physical Location & Seq

Genomic Coordinates Forward (+)

128761 .. 142762

Gene Structure 14002 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1000700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 597 bp

ATGTTTGCTTCTCAACTTTCAAGAATCGGATTTTTGATACTGAAACAGCTCTCCAGAGGCAAATCCGCATCTTTAACCAGATCAGGATATACGAGTAATTCTTACAGACAATGCTTGCAAACTCTGTTTGATCACCAGTCTGAAACTAAGTTGTTGCGAGCAACCATCTTTGAGAACCGTTATCTTTTTTATACCAGTAGTGCCAGCCATTCTTCAAATGAAGGGATTGAGCAGAAAGAAAAGATATCAGTGACATTTGTTGATAAGGATGGAGAGGAAAAACATATCAAGGTTCCTACTGGAATGTCTATTCTTGAAGCTGCCCATGAAAATGATATAGAACTTGAAGGAGCATGCGAAGGTTCACTTGCCTGTTCGACATGTCATGTGATTGTGATGGACGTGGACTATTACAACAAATTAGAAGACCCAATTGATGAGGAAAATGATATGTTGGACTTGGCCTTTGGACTTACTGAAACGTCTCGCTTGGGTTGTCAAGTCATTGCAAAACCTGAACTTGATGGAATTCGTTTAGCAATTCCAGCTGCCACCCGAAACTTTGCTGTCGATGGCTATGTTCCTAAACCACACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006091 GO:0008150 GO:0008152 GO:0009856 GO:0009987 GO:0022414 GO:0022900 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0044706 GO:0048856 GO:0048868 GO:0051704 GO:0055114

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Fer2

Protein Analysis

199

Amino Acids

22.04

Weight (kDa)

5.14

Isoelectric Point (pI)

43.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Fer2	PF00111	88 - 170	5.8e-12	2Fe-2S iron-sulfur cluster binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0004704)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G21090 AT4G21090 AT4G21090
fragaria_vesca	FvH4_4g10710 FvH4_4g10710 FvH4_4g10710 FvH4_4g10710 FvH4_4g10710
malus_domestica	MD12G1000100.v1.1 MD14G1000700.v1.1
prunus_persica	Prupe.7G000600_v2.0.a1 Prupe.7G000600_v2.0.a1 Prupe.7G000600_v2.0.a1 Prupe.7G000600_v2.0.a1
pyrus_communis	pycom12g00020
rosa_chinensis	RchiOBHm_Chr4g0406961 RchiOBHm_Chr6g0270801
rosa_laevigata	RLG00000008745
rosa_multiflora	Rmu_sc0000600.1_g000021 Rmu_sc0000642.1_g000023
rosa_roxburghii	Rroxscaffold_5G00351710
rosa_rugosa	Rorug04G0075800 Rorug04G0075800
rosa_samantha	Rh4AG141700 Rh4BG138800 Rh4CG148500 Rh4DG135800 Rh6AG176000 Rh6BG179200
rosa_wichuraiana	Rw4G011540 Rw6G014980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	1 cut(s)	66
AcsI	RAATTY	1 cut(s)	528
AflIII	ACRYGT	1 cut(s)	380
AgsI	TTSAA	4 cut(s)	21, 216, 317, 347
AjiI	CACGTC	1 cut(s)	403
AluBI	AGCT	3 cut(s)	49, 320, 548
AluI	AGCT	3 cut(s)	49, 320, 548
Alw26I	GTCTC	1 cut(s)	489
AoxI	GGCC	1 cut(s)	462
ApeKI	GCWGC	2 cut(s)	320, 548
ApoI	RAATTY	1 cut(s)	528
ArsI	GACNNNNNNTTYG	2 cut(s)	449, 481
AsuHPI	GGTGA	1 cut(s)	125
BbsI	GAAGAC	1 cut(s)	432
BbvI	GCAGC	2 cut(s)	307, 535
BccI	CCATC	5 cut(s)	173, 263, 391, 518, 566
BclI	TGATCA	1 cut(s)	130
BcoDI	GTCTC	1 cut(s)	489
BfaI	CTAG	1 cut(s)	595
BisI	GCNGC	2 cut(s)	321, 549
BlsI	GCNGC	2 cut(s)	322, 550
BmgBI	CACGTC	1 cut(s)	403
BmiI	GGNNCC	1 cut(s)	294
BmsI	GCATC	1 cut(s)	77
BpiI	GAAGAC	1 cut(s)	432
BpmI	CTGGAG	1 cut(s)	37
Bse1I	ACTGG	3 cut(s)	136, 195, 304
Bse3DI	GCAATG	1 cut(s)	504
BseGI	GGATG	1 cut(s)	274
BseMI	GCAATG	1 cut(s)	504
BseNI	ACTGG	3 cut(s)	136, 195, 304
BseXI	GCAGC	2 cut(s)	307, 535
BshFI	GGCC	1 cut(s)	464
BsmAI	GTCTC	1 cut(s)	489
BsmBI	CGTCTC	1 cut(s)	489
BsnI	GGCC	1 cut(s)	464
Bsp143I	GATC	2 cut(s)	80, 130
BspACI	CCGC	1 cut(s)	66
BspANI	GGCC	1 cut(s)	464
BspLI	GGNNCC	1 cut(s)	294
BsrDI	GCAATG	1 cut(s)	504
BsrI	ACTGG	3 cut(s)	136, 195, 304
BssMI	GATC	2 cut(s)	80, 130
Bst4CI	ACNGT	1 cut(s)	179
BstC8I	GCNNGC	4 cut(s)	116, 159, 205, 355
BstDEI	CTNAG	1 cut(s)	147
BstF5I	GGATG	1 cut(s)	274
BstKTI	GATC	2 cut(s)	83, 133
BstMAI	GTCTC	1 cut(s)	489
BstMBI	GATC	2 cut(s)	80, 130
BstMWI	GCNNNNNNNGC	1 cut(s)	545
BstNSI	RCATGY	2 cut(s)	357, 384
BstV1I	GCAGC	2 cut(s)	307, 535
BstV2I	GAAGAC	1 cut(s)	432
BsuRI	GGCC	1 cut(s)	464
BtrI	CACGTC	1 cut(s)	403
BtsCI	GGATG	1 cut(s)	274
BtsIMutI	CAGTG	1 cut(s)	255
Cac8I	GCNNGC	4 cut(s)	116, 159, 205, 355
CviAII	CATG	4 cut(s)	326, 354, 381, 386
CviJI	RGCY	6 cut(s)	49, 207, 320, 464, 548, 576
CviKI_1	RGCY	6 cut(s)	49, 207, 320, 464, 548, 576
DdeI	CTNAG	1 cut(s)	147
DpnI	GATC	2 cut(s)	82, 132
DpnII	GATC	2 cut(s)	80, 130
Eco32I	GATATC	1 cut(s)	246
EcoRI	GAATTC	1 cut(s)	528
EcoRV	GATATC	1 cut(s)	246
Esp3I	CGTCTC	1 cut(s)	489
FaeI	CATG	4 cut(s)	329, 357, 384, 389
FalI	AAGNNNNNCTT	2 cut(s)	351, 383
FatI	CATG	4 cut(s)	325, 353, 380, 385
FbaI	TGATCA	1 cut(s)	130
Fnu4HI	GCNGC	2 cut(s)	321, 549
FokI	GGATG	1 cut(s)	281
Fsp4HI	GCNGC	2 cut(s)	321, 549
FspBI	CTAG	1 cut(s)	595
GluI	GCNGC	2 cut(s)	321, 549
GsuI	CTGGAG	1 cut(s)	37
HaeIII	GGCC	1 cut(s)	464
Hin1II	CATG	4 cut(s)	329, 357, 384, 389
HinfI	GANTC	1 cut(s)	24
HphI	GGTGA	1 cut(s)	125
Hpy166II	GTNNAC	2 cut(s)	365, 406
Hpy188I	TCNGA	2 cut(s)	29, 142
Hpy188III	TCNNGA	4 cut(s)	21, 54, 84, 314
Hpy8I	GTNNAC	2 cut(s)	365, 406
HpyAV	CCTTC	3 cut(s)	215, 341, 353
HpyCH4III	ACNGT	1 cut(s)	179
HpyCH4IV	ACGT	2 cut(s)	402, 482
HpyCH4V	TGCA	2 cut(s)	118, 509
HpyF10VI	GCNNNNNNNGC	1 cut(s)	545
HpyF3I	CTNAG	1 cut(s)	147
HpySE526I	ACGT	2 cut(s)	402, 482
Hsp92II	CATG	4 cut(s)	329, 357, 384, 389
Ksp22I	TGATCA	1 cut(s)	130
Kzo9I	GATC	2 cut(s)	80, 130
LmnI	GCTCC	1 cut(s)	350
Lsp1109I	GCAGC	2 cut(s)	307, 535
LweI	GCATC	1 cut(s)	77
MaeI	CTAG	1 cut(s)	595
MaeII	ACGT	2 cut(s)	402, 482
MaeIII	GTNAC	1 cut(s)	250
MalI	GATC	2 cut(s)	82, 132
MboI	GATC	2 cut(s)	80, 130
MboII	GAAGA	2 cut(s)	204, 437
MfeI	CAATTG	1 cut(s)	432
MluCI	AATT	5 cut(s)	97, 419, 432, 528, 540
MmeI	TCCRAC	1 cut(s)	435
MnlI	CCTC	3 cut(s)	50, 268, 433
MseI	TTAA	1 cut(s)	74
MslI	CAYNNNNRTG	1 cut(s)	330
MspA1I	CMGCKG	1 cut(s)	548
MunI	CAATTG	1 cut(s)	432
MwoI	GCNNNNNNNGC	1 cut(s)	545
NdeII	GATC	2 cut(s)	80, 130
NlaIII	CATG	4 cut(s)	329, 357, 384, 389
NlaIV	GGNNCC	1 cut(s)	294
NmuCI	GTSAC	1 cut(s)	250
NspI	RCATGY	2 cut(s)	357, 384
PaeI	GCATGC	1 cut(s)	357
PciI	ACATGT	1 cut(s)	380
PfeI	GAWTC	1 cut(s)	24
PkrI	GCNGC	2 cut(s)	322, 550
PscI	ACATGT	1 cut(s)	380
PspN4I	GGNNCC	1 cut(s)	294
PvuII	CAGCTG	1 cut(s)	548
RseI	CAYNNNNRTG	1 cut(s)	330
SaqAI	TTAA	1 cut(s)	74
SatI	GCNGC	2 cut(s)	321, 549
Sau3AI	GATC	2 cut(s)	80, 130
SetI	ASST	8 cut(s)	51, 294, 322, 364, 405, 485, 517, 550
SfaNI	GCATC	1 cut(s)	77
SmiMI	CAYNNNNRTG	1 cut(s)	330
SphI	GCATGC	1 cut(s)	357
Sse9I	AATT	5 cut(s)	97, 419, 432, 528, 540
SsiI	CCGC	1 cut(s)	66
SspMI	CTAG	1 cut(s)	595
TaaI	ACNGT	1 cut(s)	179
TaiI	ACGT	2 cut(s)	405, 485
TaqI	TCGA	2 cut(s)	377, 570
TasI	AATT	5 cut(s)	97, 419, 432, 528, 540
TfiI	GAWTC	1 cut(s)	24
Tru1I	TTAA	1 cut(s)	74
Tru9I	TTAA	1 cut(s)	74
TscAI	CASTG	1 cut(s)	255
TseFI	GTSAC	1 cut(s)	250
TseI	GCWGC	2 cut(s)	320, 548
Tsp45I	GTSAC	1 cut(s)	250
TspDTI	ATGAA	2 cut(s)	234, 342
TspRI	CASTG	1 cut(s)	255
XapI	RAATTY	1 cut(s)	528
XceI	RCATGY	2 cut(s)	357, 384
XspI	CTAG	1 cut(s)	595

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.