Rroxscaffold_5G00351710

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0004704
Physical Location & Seq
Forward (+)
28239291 .. 28242731
3441 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00351710.1

Sequence Viewer

Length: 591 bp
ATGTTGGGCTCTCGGGCTTCAAGATTTGGACTTTTGATACTGAAACACCTTACCGGAGGAGGCAAATCCATTAGTACGCCAACATATGCAACTCGTTCCTTCAGCCAATTCTTGCAACCTCGGTATGATCAACAGTCCGGGACCAAGTTGTTCAGGGGAGCCATCTTTCAGAAGCATAATCATTTTTCTACCACCACTGCCAGCTCTTCAGATCAAGGGACTGAGCAGAAAGAAACGATATCTGTGACCTTTGTTGACAAGGATGGAGACGAAAAACATATAAAGGTTCCAATTGGGATGTCTATGCTAGAAGCTGCTCATCAAAATGATATAGAACTTGAAGGAGCCTGTGAAGGTTCACTTGCCTGTTCAACATGTCATGTGATTTTAATGGACGTGGAATATTACAACAAATTAGAAGACCCAACTGATGAGGAAAATGATATGTTGGACTTGGCATTTGGACTTACAGAAACGTCTCGTCTGGGTTGCCAAGTCATTGCAAGCCCCGAACTTGATGGAGTTCGTTTAGCTATTCCTGCTGCCACCCGAAATTTTGCTGTTGATGGCTATGTACCAAAACCACACTAG

Protein Analysis

196

Amino Acids

21.46

Weight (kDa)

5.31

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 86 - 168 4.2e-11 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 2 cut(s) 85, 192
AfaI GTAC 2 cut(s) 76, 576
AflIII ACRYGT 1 cut(s) 374
AgsI TTSAA 3 cut(s) 21, 341, 372
AjiI CACGTC 1 cut(s) 397
AluBI AGCT 3 cut(s) 204, 314, 533
AluI AGCT 3 cut(s) 204, 314, 533
Alw26I GTCTC 2 cut(s) 261, 483
Ama87I CYCGRG 1 cut(s) 12
ApeKI GCWGC 2 cut(s) 314, 542
ApoI RAATTY 1 cut(s) 553
ArsI GACNNNNNNTTYG 2 cut(s) 443, 475
AspS9I GGNCC 1 cut(s) 141
AsuC2I CCSGG 1 cut(s) 139
AvaI CYCGRG 1 cut(s) 12
AvaII GGWCC 1 cut(s) 141
BanII GRGCYC 1 cut(s) 11
BbsI GAAGAC 1 cut(s) 426
BbvI GCAGC 2 cut(s) 301, 529
BccI CCATC 4 cut(s) 170, 257, 512, 560
BclI TGATCA 1 cut(s) 127
BcnI CCSGG 1 cut(s) 139
BcoDI GTCTC 2 cut(s) 261, 483
BfaI CTAG 2 cut(s) 308, 589
BisI GCNGC 2 cut(s) 315, 543
BlsI GCNGC 2 cut(s) 316, 544
Bme1390I CCNGG 1 cut(s) 139
Bme18I GGWCC 1 cut(s) 141
BmeT110I CYCGRG 1 cut(s) 12
BmgBI CACGTC 1 cut(s) 397
BmgT120I GGNCC 1 cut(s) 141
BmiI GGNNCC 4 cut(s) 142, 160, 288, 346
BmrFI CCNGG 1 cut(s) 139
BpiI GAAGAC 1 cut(s) 426
BpuMI CCSGG 1 cut(s) 139
BsaJI CCNNGG 1 cut(s) 119
BsaWI WCCGGW 1 cut(s) 53
Bse3DI GCAATG 1 cut(s) 498
BseDI CCNNGG 1 cut(s) 119
BseGI GGATG 2 cut(s) 268, 303
BseMI GCAATG 1 cut(s) 498
BseMII CTCAG 1 cut(s) 213
BseRI GAGGAG 1 cut(s) 72
BseXI GCAGC 2 cut(s) 301, 529
BsiHKCI CYCGRG 1 cut(s) 12
BsiSI CCGG 2 cut(s) 54, 138
BslFI GGGAC 2 cut(s) 154, 232
BsmAI GTCTC 2 cut(s) 261, 483
BsmBI CGTCTC 2 cut(s) 261, 483
BsmFI GGGAC 2 cut(s) 154, 232
BsoBI CYCGRG 1 cut(s) 12
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 2 cut(s) 127, 211
BspCNI CTCAG 1 cut(s) 214
BspLI GGNNCC 4 cut(s) 142, 160, 288, 346
BspQI GCTCTTC 1 cut(s) 211
BsrDI GCAATG 1 cut(s) 498
BssECI CCNNGG 1 cut(s) 119
BssMI GATC 2 cut(s) 127, 211
Bst4CI ACNGT 1 cut(s) 135
Bst6I CTCTTC 1 cut(s) 211
BstC8I GCNNGC 2 cut(s) 202, 505
BstDEI CTNAG 1 cut(s) 222
BstF5I GGATG 2 cut(s) 268, 303
BstKTI GATC 2 cut(s) 130, 214
BstMAI GTCTC 2 cut(s) 261, 483
BstMBI GATC 2 cut(s) 127, 211
BstMWI GCNNNNNNNGC 1 cut(s) 539
BstNSI RCATGY 1 cut(s) 378
BstSCI CCNGG 1 cut(s) 137
BstV1I GCAGC 2 cut(s) 301, 529
BstV2I GAAGAC 1 cut(s) 426
BtrI CACGTC 1 cut(s) 397
BtsCI GGATG 2 cut(s) 268, 303
BtsI GCAGTG 1 cut(s) 195
BtsIMutI CAGTG 1 cut(s) 195
Cac8I GCNNGC 2 cut(s) 202, 505
Cfr13I GGNCC 1 cut(s) 141
Csp6I GTAC 2 cut(s) 75, 575
CviAII CATG 2 cut(s) 375, 380
CviQI GTAC 2 cut(s) 75, 575
DdeI CTNAG 1 cut(s) 222
DpnI GATC 2 cut(s) 129, 213
DpnII GATC 2 cut(s) 127, 211
Eam1104I CTCTTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 211
Eco24I GRGCYC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 141
Eco57I CTGAAG 2 cut(s) 85, 192
Eco88I CYCGRG 1 cut(s) 12
EcoRV GATATC 1 cut(s) 240
EcoT38I GRGCYC 1 cut(s) 11
Esp3I CGTCTC 2 cut(s) 261, 483
FaeI CATG 2 cut(s) 378, 383
FalI AAGNNNNNCTT 2 cut(s) 345, 377
FaqI GGGAC 2 cut(s) 154, 232
FatI CATG 2 cut(s) 374, 379
FauNDI CATATG 1 cut(s) 85
FbaI TGATCA 1 cut(s) 127
Fnu4HI GCNGC 2 cut(s) 315, 543
FokI GGATG 2 cut(s) 275, 310
FriOI GRGCYC 1 cut(s) 11
Fsp4HI GCNGC 2 cut(s) 315, 543
FspBI CTAG 2 cut(s) 308, 589
GluI GCNGC 2 cut(s) 315, 543
HapII CCGG 2 cut(s) 54, 138
Hin1II CATG 2 cut(s) 378, 383
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HpaII CCGG 2 cut(s) 54, 138
Hpy166II GTNNAC 2 cut(s) 256, 359
Hpy188I TCNGA 2 cut(s) 171, 211
Hpy188III TCNNGA 1 cut(s) 21
Hpy8I GTNNAC 2 cut(s) 256, 359
HpyAV CCTTC 3 cut(s) 109, 335, 347
HpyCH4III ACNGT 1 cut(s) 135
HpyCH4IV ACGT 2 cut(s) 396, 476
HpyCH4V TGCA 3 cut(s) 89, 115, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 539
HpyF3I CTNAG 1 cut(s) 222
HpySE526I ACGT 2 cut(s) 396, 476
Hsp92II CATG 2 cut(s) 378, 383
Ksp22I TGATCA 1 cut(s) 127
Kzo9I GATC 2 cut(s) 127, 211
LguI GCTCTTC 1 cut(s) 211
LmnI GCTCC 2 cut(s) 158, 344
LpnPI CCDG 8 cut(s) 67, 139, 151, 214, 361, 379, 470, 552
Lsp1109I GCAGC 2 cut(s) 301, 529
MaeI CTAG 2 cut(s) 308, 589
MaeII ACGT 2 cut(s) 396, 476
MaeIII GTNAC 1 cut(s) 244
MalI GATC 2 cut(s) 129, 213
MboI GATC 2 cut(s) 127, 211
MboII GAAGA 2 cut(s) 198, 431
MfeI CAATTG 1 cut(s) 291
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 4 cut(s) 107, 291, 413, 553
MmeI TCCRAC 1 cut(s) 429
MnlI CCTC 4 cut(s) 50, 53, 129, 427
MseI TTAA 1 cut(s) 389
MslI CAYNNNNRTG 1 cut(s) 324
MspI CCGG 2 cut(s) 54, 138
MspR9I CCNGG 1 cut(s) 139
MunI CAATTG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 539
NciI CCSGG 1 cut(s) 139
NdeI CATATG 1 cut(s) 85
NdeII GATC 2 cut(s) 127, 211
NlaIII CATG 2 cut(s) 378, 383
NlaIV GGNNCC 4 cut(s) 142, 160, 288, 346
NmuCI GTSAC 1 cut(s) 244
NspI RCATGY 1 cut(s) 378
PciI ACATGT 1 cut(s) 374
PciSI GCTCTTC 1 cut(s) 211
PfoI TCCNGGA 1 cut(s) 137
PkrI GCNGC 2 cut(s) 316, 544
PscI ACATGT 1 cut(s) 374
PspN4I GGNNCC 4 cut(s) 142, 160, 288, 346
PspPI GGNCC 1 cut(s) 141
RsaI GTAC 2 cut(s) 76, 576
RsaNI GTAC 2 cut(s) 75, 575
RseI CAYNNNNRTG 1 cut(s) 324
SapI GCTCTTC 1 cut(s) 211
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 2 cut(s) 315, 543
Sau3AI GATC 2 cut(s) 127, 211
Sau96I GGNCC 1 cut(s) 141
ScrFI CCNGG 1 cut(s) 139
SduI GDGCHC 1 cut(s) 11
SinI GGWCC 1 cut(s) 141
SmiMI CAYNNNNRTG 1 cut(s) 324
Sse9I AATT 4 cut(s) 107, 291, 413, 553
SspI AATATT 1 cut(s) 404
SspMI CTAG 2 cut(s) 308, 589
StyD4I CCNGG 1 cut(s) 137
TaaI ACNGT 1 cut(s) 135
TaiI ACGT 2 cut(s) 399, 479
TasI AATT 4 cut(s) 107, 291, 413, 553
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 2 cut(s) 314, 542
Tsp45I GTSAC 1 cut(s) 244
TspRI CASTG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 141
XapI RAATTY 1 cut(s) 553
XceI RCATGY 1 cut(s) 378
XspI CTAG 2 cut(s) 308, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.