Rh4DG135800

2Fe-2S iron-sulfur cluster binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004704
Physical Location & Seq
Forward (+)
27065379 .. 27067973
2595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG135800.1

Sequence Viewer

Length: 633 bp
ATGGAGAGTGCGTCACCCGTTGAATACGGTAGCTGCCGGAGCTCTTCCTGCCGCCACTCAATTTCGACACCAATCCAGTCCAGATTGAAGCTTACCGGAGGAGGCAATTCCATTAGTAGACCAACATATGCAACTCGTTCTTTCAGCCAATTCTTGCAACCTCGGTATGATCAACATTCCGGGACCAAGTTGTTCAGGGGAGCCATCTTTCAGAAGCATAATCATTTTTCTACCACCACTGCCAGCTCTTCAGATCAAGGGACTGAGCAGAAAGAAACGATATCTGTGACCTTTGTTGACAAGGATGGAGACGAAAAACATATAAAGGTTCCAATTGGGATGTCTATGCTAGAAGCTGCTCATCAAAATGATATAGAACTTGAAGGAGCCTGTGAAGGTTCACTTGCCTGTTCAACATGTCATGTGATTTTAATGGACGTGGAATATTACAACAAATTAGAAGACCCAACTGATGAGGAAAATGATATGTTGGACTTGGCATTTGGACTTACAGAAACGTCTCGTCTGGGTTGCCAAGTCATTGCAAGCCCCGAACTTGATGGAGTTCGTTTAGCTATTCCTGCTGCCACCCGAAATTTTGCTGTTGATGGCTATGTACCAAAACCACACTAG

Protein Analysis

210

Amino Acids

23.0

Weight (kDa)

5.32

Isoelectric Point (pI)

53.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fer2 PF00111 100 - 182 4.9e-11 2Fe-2S iron-sulfur cluster binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 118
AciI CCGC 1 cut(s) 52
AcsI RAATTY 1 cut(s) 595
AcuI CTGAAG 1 cut(s) 234
AfaI GTAC 1 cut(s) 618
AflIII ACRYGT 1 cut(s) 416
AgsI TTSAA 4 cut(s) 23, 88, 383, 414
AjiI CACGTC 1 cut(s) 439
AluBI AGCT 6 cut(s) 33, 42, 91, 246, 356, 575
AluI AGCT 6 cut(s) 33, 42, 91, 246, 356, 575
Alw21I GWGCWC 1 cut(s) 44
Alw26I GTCTC 2 cut(s) 303, 525
ApeKI GCWGC 3 cut(s) 33, 356, 584
ApoI RAATTY 1 cut(s) 595
ArsI GACNNNNNNTTYG 2 cut(s) 485, 517
AspS9I GGNCC 1 cut(s) 183
AsuC2I CCSGG 1 cut(s) 181
AsuHPI GGTGA 1 cut(s) 6
AvaII GGWCC 1 cut(s) 183
BanII GRGCYC 1 cut(s) 44
BbsI GAAGAC 1 cut(s) 468
Bbv12I GWGCWC 1 cut(s) 44
BbvI GCAGC 3 cut(s) 20, 343, 571
BccI CCATC 4 cut(s) 212, 299, 554, 602
BclI TGATCA 1 cut(s) 169
BcnI CCSGG 1 cut(s) 181
BcoDI GTCTC 2 cut(s) 303, 525
BfaI CTAG 2 cut(s) 350, 631
BisI GCNGC 4 cut(s) 34, 52, 357, 585
BlsI GCNGC 4 cut(s) 35, 53, 358, 586
Bme1390I CCNGG 1 cut(s) 181
Bme18I GGWCC 1 cut(s) 183
BmgBI CACGTC 1 cut(s) 439
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 4 cut(s) 184, 202, 330, 388
BmrFI CCNGG 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 468
BpuMI CCSGG 1 cut(s) 181
BsaJI CCNNGG 1 cut(s) 161
BsaWI WCCGGW 1 cut(s) 95
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 76
Bse3DI GCAATG 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 161
BseGI GGATG 2 cut(s) 310, 345
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 114
BseXI GCAGC 3 cut(s) 20, 343, 571
BsiHKAI GWGCWC 1 cut(s) 44
BsiSI CCGG 3 cut(s) 37, 96, 180
BslFI GGGAC 2 cut(s) 196, 274
BsmAI GTCTC 2 cut(s) 303, 525
BsmBI CGTCTC 2 cut(s) 303, 525
BsmFI GGGAC 2 cut(s) 196, 274
Bsp1286I GDGCHC 1 cut(s) 44
Bsp143I GATC 2 cut(s) 169, 253
BspACI CCGC 1 cut(s) 52
BspCNI CTCAG 1 cut(s) 256
BspLI GGNNCC 4 cut(s) 184, 202, 330, 388
BspQI GCTCTTC 2 cut(s) 49, 253
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 161
BssMI GATC 2 cut(s) 169, 253
Bst4CI ACNGT 1 cut(s) 29
Bst6I CTCTTC 2 cut(s) 49, 253
BstC8I GCNNGC 2 cut(s) 244, 547
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 2 cut(s) 310, 345
BstKTI GATC 2 cut(s) 172, 256
BstMAI GTCTC 2 cut(s) 303, 525
BstMBI GATC 2 cut(s) 169, 253
BstMWI GCNNNNNNNGC 3 cut(s) 39, 48, 581
BstNSI RCATGY 1 cut(s) 420
BstSCI CCNGG 1 cut(s) 179
BstV1I GCAGC 3 cut(s) 20, 343, 571
BstV2I GAAGAC 1 cut(s) 468
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 2 cut(s) 310, 345
BtsI GCAGTG 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 2 cut(s) 244, 547
Cfr13I GGNCC 1 cut(s) 183
Csp6I GTAC 1 cut(s) 617
CviAII CATG 2 cut(s) 417, 422
CviQI GTAC 1 cut(s) 617
DdeI CTNAG 1 cut(s) 264
DpnI GATC 2 cut(s) 171, 255
DpnII GATC 2 cut(s) 169, 253
Eam1104I CTCTTC 2 cut(s) 49, 253
EarI CTCTTC 2 cut(s) 49, 253
Ecl136II GAGCTC 1 cut(s) 42
Eco24I GRGCYC 1 cut(s) 44
Eco32I GATATC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 183
Eco53kI GAGCTC 1 cut(s) 42
Eco57I CTGAAG 1 cut(s) 234
EcoICRI GAGCTC 1 cut(s) 42
EcoRV GATATC 1 cut(s) 282
EcoT38I GRGCYC 1 cut(s) 44
Esp3I CGTCTC 2 cut(s) 303, 525
FaeI CATG 2 cut(s) 420, 425
FalI AAGNNNNNCTT 2 cut(s) 387, 419
FaqI GGGAC 2 cut(s) 196, 274
FatI CATG 2 cut(s) 416, 421
FauNDI CATATG 1 cut(s) 127
FbaI TGATCA 1 cut(s) 169
FblI GTMKAC 1 cut(s) 118
Fnu4HI GCNGC 4 cut(s) 34, 52, 357, 585
FokI GGATG 2 cut(s) 317, 352
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 4 cut(s) 34, 52, 357, 585
FspBI CTAG 2 cut(s) 350, 631
GluI GCNGC 4 cut(s) 34, 52, 357, 585
HapII CCGG 3 cut(s) 37, 96, 180
Hin1II CATG 2 cut(s) 420, 425
HincII GTYRAC 1 cut(s) 298
HindII GTYRAC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 89
HpaII CCGG 3 cut(s) 37, 96, 180
HphI GGTGA 1 cut(s) 6
Hpy166II GTNNAC 3 cut(s) 119, 298, 401
Hpy188I TCNGA 2 cut(s) 213, 253
Hpy188III TCNNGA 1 cut(s) 81
Hpy8I GTNNAC 3 cut(s) 119, 298, 401
HpyAV CCTTC 2 cut(s) 377, 389
HpyCH4III ACNGT 1 cut(s) 29
HpyCH4IV ACGT 2 cut(s) 438, 518
HpyCH4V TGCA 3 cut(s) 131, 157, 545
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 48, 581
HpyF3I CTNAG 1 cut(s) 264
HpySE526I ACGT 2 cut(s) 438, 518
Hsp92II CATG 2 cut(s) 420, 425
Ksp22I TGATCA 1 cut(s) 169
Kzo9I GATC 2 cut(s) 169, 253
LguI GCTCTTC 2 cut(s) 49, 253
LmnI GCTCC 3 cut(s) 39, 200, 386
Lsp1109I GCAGC 3 cut(s) 20, 343, 571
MaeI CTAG 2 cut(s) 350, 631
MaeII ACGT 2 cut(s) 438, 518
MaeIII GTNAC 2 cut(s) 12, 286
MalI GATC 2 cut(s) 171, 255
MboI GATC 2 cut(s) 169, 253
MboII GAAGA 3 cut(s) 36, 240, 473
MfeI CAATTG 1 cut(s) 333
MhlI GDGCHC 1 cut(s) 44
MluCI AATT 6 cut(s) 60, 106, 149, 333, 455, 595
MmeI TCCRAC 1 cut(s) 471
MnlI CCTC 4 cut(s) 92, 95, 171, 469
MseI TTAA 1 cut(s) 431
MslI CAYNNNNRTG 1 cut(s) 366
MspI CCGG 3 cut(s) 37, 96, 180
MspR9I CCNGG 1 cut(s) 181
MunI CAATTG 1 cut(s) 333
MwoI GCNNNNNNNGC 3 cut(s) 39, 48, 581
NciI CCSGG 1 cut(s) 181
NdeI CATATG 1 cut(s) 127
NdeII GATC 2 cut(s) 169, 253
NlaIII CATG 2 cut(s) 420, 425
NlaIV GGNNCC 4 cut(s) 184, 202, 330, 388
NmuCI GTSAC 2 cut(s) 12, 286
NspI RCATGY 1 cut(s) 420
PciI ACATGT 1 cut(s) 416
PciSI GCTCTTC 2 cut(s) 49, 253
PfoI TCCNGGA 1 cut(s) 179
PkrI GCNGC 4 cut(s) 35, 53, 358, 586
PscI ACATGT 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 44
PspN4I GGNNCC 4 cut(s) 184, 202, 330, 388
PspPI GGNCC 1 cut(s) 183
RsaI GTAC 1 cut(s) 618
RsaNI GTAC 1 cut(s) 617
RseI CAYNNNNRTG 1 cut(s) 366
SacI GAGCTC 1 cut(s) 44
SapI GCTCTTC 2 cut(s) 49, 253
SaqAI TTAA 1 cut(s) 431
SatI GCNGC 4 cut(s) 34, 52, 357, 585
Sau3AI GATC 2 cut(s) 169, 253
Sau96I GGNCC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 181
SduI GDGCHC 1 cut(s) 44
SinI GGWCC 1 cut(s) 183
SmiMI CAYNNNNRTG 1 cut(s) 366
Sse9I AATT 6 cut(s) 60, 106, 149, 333, 455, 595
SsiI CCGC 1 cut(s) 52
SspI AATATT 1 cut(s) 446
SspMI CTAG 2 cut(s) 350, 631
SstI GAGCTC 1 cut(s) 44
StyD4I CCNGG 1 cut(s) 179
TaaI ACNGT 1 cut(s) 29
TaiI ACGT 2 cut(s) 441, 521
TaqI TCGA 1 cut(s) 65
TasI AATT 6 cut(s) 60, 106, 149, 333, 455, 595
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 431
Tru9I TTAA 1 cut(s) 431
TscAI CASTG 1 cut(s) 244
TseFI GTSAC 2 cut(s) 12, 286
TseI GCWGC 3 cut(s) 33, 356, 584
Tsp45I GTSAC 2 cut(s) 12, 286
TspRI CASTG 1 cut(s) 244
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 1 cut(s) 595
XceI RCATGY 1 cut(s) 420
XmiI GTMKAC 1 cut(s) 118
XspI CTAG 2 cut(s) 350, 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.