Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0009791

Physical Location & Seq

Genomic Coordinates Forward (+)

5577659 .. 5581000

Gene Structure 3342 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1054900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1854 bp

ATGGCAGATGTCACGAAGCAGCAGCACCTCCCCATCAAGGGCGGAACTCTGATCTCGGACGTCAACAGCCTCTTCTCTATCCTCAACACCAGAAACACTGTGGCTTTTGCATATGGCATCATGTTTGCTTTCATCGCCTTGACTGTTTTCATGACCTTCAGTCCCTCCCCGAGCTACGCTTCTCCATATTTTGCAAGCATTATCTCTACTTCAAATTCTACTACTTCCTCTTCTGGTTCGAGCAGTTCACTTTTCTCCTTCTTCTTCCCCAATAGTACTTCTTCATCTCCGCCGCAGCAAGATCGAACCGTTTTTTCTCCTCCGCCGCAAACAGAGCCGTCTAGATCTGCTAACACCACCGCCGCCCCCGCAGCATCTGCCACTGTGAATAAACAACCCACTATTTTGAACCCGAATCAGACCTCAAACGTCCAGCCAAAACCTCCAGTCACACCCAAGAATAGTACTACTTCACCTGAACCGACAACCCGTTTGAAAAACCAGACCGACAACACCCACAATTCTGATAAGGCCACAGTTTTGACAGCCAATCAGACCGGAACCACCGCCCCAAAAACCCCGGTGGCGGCCGGGAATGGAAGCGCCGATCCGGCACCGAAATCGAGTTCCGCGAACAAGAGCGTTGCAGACAAGGGTGTGGAGAAGAATTTAACTGCTCCCCTGTTGAAAAAACAGAGCAATGAAACGAATTCCGGCGTGCCGGCTGAGCAGGGGAAGCAGGGGAGTGATGATTTGGTGAAGTCTTTGATGAATTGTGATATGTTACATGGAGAGTGGGTGAAGGACGATTCGTATCCGCTATACAAACCTGGGTCTTGTCCTCTGATTGATGAACAGTTCAATTGTATTCTCAACGGCAGACCTGATAAGGATTACCAAAAATTCAAATGGAAGCCTAAGGATTGCACTTTACCAAGGTTGGATGGAAGTCATATGTTGGAGTTGTTGAGAGGAAAGCGCCTGGTATTCGTTGGTGATTCTCTGAATAGGAATATGTGGGAGTCTCTGGTTTGCATCCTTAGAAACTCGGTGAAAGATAAGAGCAAGGTTTTCGAAGCCAATGGGAGAACCCATTTTCGTGGGGAAGCTTCGTATTCCTTCATATTCAAAGACTATAACTGCACGGTGGAGTTCTTCGTGTCTCCCTTCTTAGTTCGAGAATGGGAAATGCCGGAGAAAGATGGATCAAAGAGAGAAACACTTCGTCTTGACTTAGTAGGGAGATCTTCCGATCTATATAAAGATGCTGATATTGTCATCTTCAACACCGGACACTGGTGGACTCATGAGAAAACCTCCAAAGGGAAAGACTATTACCAAGAAGGTAGCCATGTCTATGGTGAATTGAATGTTCTTGAGGCGTTTCGGAAAGCTTTAACTACGTGGGCAGGATGGGTTGATGCCAAGGTTGATCCGAAGAAAAGTATCGTCTTCTTTCGGGGCTATTCTGCTTCCCATTTCAGTGGTGGGCAGTGGAATTCTGGTGGCCAATGTGACAGTGAGACAGTTCCCATCTTCAATGAGAAGTATCTGAGGCCGTACCCGCCCAAGATGCTGGTACTGGAGAAGGTGTTGAGAAACATGAAAAGCCATGTCACATATCTCAATATCACAAGAATGACAGATTTCCGGAAGGACGGTCACCCCTCAATCTACCGGAAGCAAAAACTGTCCAAGGAAGAAAGGACATCACCTAAGAGCTTCCAGGACTGCAGCCATTGGTGCCTTCCTGGTGTTCCTGACGCATGGAATGAGGTTCTCTACGCCGAGCTCCTAGTAAAGCTGTACCAAAATAAGCAGCAGCACCTGCAACGGCAGAAGAAGAGAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

618

Amino Acids

68.98

Weight (kDa)

9.24

Isoelectric Point (pI)

41.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	258 - 309	1.3e-22	PMR5 N terminal Domain
PC-Esterase	PF13839	311 - 596	8.5e-101	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009791)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12060 AT5G06700
fragaria_vesca	FvH4_6g22040
malus_domestica	MD12G1055700.v1.1 MD14G1054900.v1.1
prunus_persica	Prupe.7G079400_v2.0.a1
pyrus_communis	pycom12g04850 pycom14g04530
rosa_chinensis	RchiOBHm_Chr3g0479331
rosa_laevigata	RLG00000023584
rosa_multiflora	Rmu_co8488735.1_g000001 Rmu_sc0006763.1_g000008 Rmu_sc0006763.1_g000009
rosa_roxburghii	Rroxscaffold_6G00402410
rosa_rugosa	Rorug03G0174400 Rorug03G0174500
rosa_samantha	Rh3AG225400 Rh3BG258200 Rh3CG254000 Rh3DG249800
rosa_wichuraiana	Rw3G020160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1836
AatII	GACGTC	1 cut(s)	63
Acc36I	ACCTGC	1 cut(s)	1836
AccB1I	GGYRCC	2 cut(s)	613, 1743
AccII	CGCG	1 cut(s)	632
AccIII	TCCGGA	1 cut(s)	1650
AclWI	GGATC	3 cut(s)	602, 1213, 1427
AcoI	YGGCCR	2 cut(s)	588, 1507
AcsI	RAATTY	5 cut(s)	214, 667, 709, 902, 1498
AcuI	CTGAAG	1 cut(s)	142
AcyI	GRCGYC	1 cut(s)	60
AfaI	GTAC	5 cut(s)	277, 466, 1562, 1581, 1808
AfiI	CCNNNNNNNGG	5 cut(s)	37, 38, 586, 1296, 1323
AhdI	GACNNNNNGTC	1 cut(s)	159
AjnI	CCWGG	4 cut(s)	829, 981, 1725, 1750
AluBI	AGCT	6 cut(s)	174, 1109, 1394, 1722, 1792, 1804
AluI	AGCT	6 cut(s)	174, 1109, 1394, 1722, 1792, 1804
Alw21I	GWGCWC	1 cut(s)	1794
Alw26I	GTCTC	3 cut(s)	1029, 1167, 1517
AlwI	GGATC	3 cut(s)	602, 1213, 1427
AlwNI	CAGNNNCTG	2 cut(s)	377, 1828
Ama87I	CYCGRG	1 cut(s)	169
Aor13HI	TCCGGA	1 cut(s)	1650
AoxI	GGCC	4 cut(s)	531, 588, 1507, 1556
ApeKI	GCWGC	7 cut(s)	19, 22, 295, 371, 1734, 1819, 1822
ApoI	RAATTY	5 cut(s)	214, 667, 709, 902, 1498
ArsI	GACNNNNNNTTYG	2 cut(s)	475, 507
Asp700I	GAANNNNTTC	1 cut(s)	1221
AspLEI	GCGC	2 cut(s)	605, 981
AsuC2I	CCSGG	2 cut(s)	581, 592
AsuHPI	GGTGA	8 cut(s)	465, 769, 811, 1007, 1063, 1373, 1655, 1704
AsuII	TTCGAA	1 cut(s)	1074
AvaI	CYCGRG	1 cut(s)	169
AxyI	CCTNAGG	1 cut(s)	918
BalI	TGGCCA	1 cut(s)	1509
BanI	GGYRCC	2 cut(s)	613, 1743
BanII	GRGCYC	1 cut(s)	1794
BbsI	GAAGAC	1 cut(s)	1444
Bbv12I	GWGCWC	1 cut(s)	1794
BbvI	GCAGC	7 cut(s)	31, 34, 307, 383, 1746, 1831, 1834
BccI	CCATC	5 cut(s)	41, 938, 1196, 1407, 1541
BceAI	ACGGC	4 cut(s)	322, 892, 1543, 1850
BcgI	CGANNNNNNTGC	6 cut(s)	284, 318, 603, 637, 1054, 1088
BciT130I	CCWGG	4 cut(s)	831, 983, 1727, 1752
BciVI	GTATCC	1 cut(s)	825
BcnI	CCSGG	2 cut(s)	581, 592
BcoDI	GTCTC	3 cut(s)	1029, 1167, 1517
BfaI	CTAG	2 cut(s)	342, 1796
BfmI	CTRYAG	1 cut(s)	1732
BfoI	RGCGCY	2 cut(s)	606, 982
BfuAI	ACCTGC	1 cut(s)	1836
BfuI	GTATCC	1 cut(s)	825
BglI	GCCNNNNNGGC	1 cut(s)	611
BglII	AGATCT	2 cut(s)	344, 1244
BlpI	GCTNAGC	1 cut(s)	726
BmcAI	AGTACT	2 cut(s)	277, 466
Bme1390I	CCNGG	6 cut(s)	581, 592, 831, 983, 1727, 1752
BmeRI	GACNNNNNGTC	1 cut(s)	159
BmeT110I	CYCGRG	1 cut(s)	169
BmiI	GGNNCC	3 cut(s)	562, 615, 1745
BmrFI	CCNGG	6 cut(s)	581, 592, 831, 983, 1727, 1752
BmsI	GCATC	6 cut(s)	126, 383, 1044, 1255, 1411, 1563
BpiI	GAAGAC	1 cut(s)	1444
BplI	GAGNNNNNCTC	2 cut(s)	1301, 1333
BpmI	CTGGAG	2 cut(s)	429, 1604
Bpu1102I	GCTNAGC	1 cut(s)	726
Bpu14I	TTCGAA	1 cut(s)	1074
BpuEI	CTTGAG	1 cut(s)	1397
BpuMI	CCSGG	2 cut(s)	581, 592
BsaAI	YACGTR	1 cut(s)	1404
BsaBI	GATNNNNATC	1 cut(s)	813
BsaHI	GRCGYC	1 cut(s)	60
BsaJI	CCNNGG	5 cut(s)	579, 830, 935, 1425, 1695
BsaWI	WCCGGW	4 cut(s)	557, 1289, 1650, 1677
BsaXI	ACNNNNNCTCC	4 cut(s)	783, 813, 1577, 1607
Bsc4I	CCNNNNNNNGG	5 cut(s)	37, 38, 586, 1296, 1323
Bse118I	RCCGGY	1 cut(s)	721
Bse1I	ACTGG	3 cut(s)	446, 1301, 1587
Bse21I	CCTNAGG	1 cut(s)	918
Bse3DI	GCAATG	1 cut(s)	706
Bse8I	GATNNNNATC	1 cut(s)	813
BseAI	TCCGGA	1 cut(s)	1650
BseBI	CCWGG	4 cut(s)	831, 983, 1727, 1752
BseDI	CCNNGG	5 cut(s)	579, 830, 935, 1425, 1695
BseGI	GGATG	3 cut(s)	949, 1035, 1418
BseJI	GATNNNNATC	1 cut(s)	813
BseLI	CCNNNNNNNGG	5 cut(s)	37, 38, 586, 1296, 1323
BseMI	GCAATG	1 cut(s)	706
BseMII	CTCAG	2 cut(s)	717, 1544
BseNI	ACTGG	3 cut(s)	446, 1301, 1587
BseRI	GAGGAG	1 cut(s)	309
BseX3I	CGGCCG	1 cut(s)	588
BseXI	GCAGC	7 cut(s)	31, 34, 307, 383, 1746, 1831, 1834
BsgI	GTGCAG	1 cut(s)	1126
Bsh1236I	CGCG	1 cut(s)	632
Bsh1285I	CGRYCG	1 cut(s)	591
BshFI	GGCC	4 cut(s)	533, 590, 1509, 1558
BshNI	GGYRCC	2 cut(s)	613, 1743
BsiEI	CGRYCG	1 cut(s)	591
BsiHKAI	GWGCWC	1 cut(s)	1794
BsiHKCI	CYCGRG	1 cut(s)	169
BslFI	GGGAC	1 cut(s)	147
BslI	CCNNNNNNNGG	5 cut(s)	37, 38, 586, 1296, 1323
BsmAI	GTCTC	3 cut(s)	1029, 1167, 1517
BsmFI	GGGAC	1 cut(s)	147
BsnI	GGCC	4 cut(s)	533, 590, 1509, 1558
BsoBI	CYCGRG	1 cut(s)	169
Bsp119I	TTCGAA	1 cut(s)	1074
Bsp1286I	GDGCHC	1 cut(s)	1794
Bsp13I	TCCGGA	1 cut(s)	1650
Bsp143I	GATC	8 cut(s)	51, 301, 344, 607, 1205, 1244, 1252, 1432
Bsp1720I	GCTNAGC	1 cut(s)	726
BspANI	GGCC	4 cut(s)	533, 590, 1509, 1558
BspCNI	CTCAG	2 cut(s)	718, 1545
BspEI	TCCGGA	1 cut(s)	1650
BspFNI	CGCG	1 cut(s)	632
BspHI	TCATGA	2 cut(s)	150, 1306
BspLI	GGNNCC	3 cut(s)	562, 615, 1745
BspMAI	CTGCAG	1 cut(s)	1736
BspMI	ACCTGC	1 cut(s)	1836
BspPI	GGATC	3 cut(s)	602, 1213, 1427
BspT104I	TTCGAA	1 cut(s)	1074
BspT107I	GGYRCC	2 cut(s)	613, 1743
BsrDI	GCAATG	1 cut(s)	706
BsrFI	RCCGGY	1 cut(s)	721
BsrI	ACTGG	3 cut(s)	446, 1301, 1587
BssAI	RCCGGY	1 cut(s)	721
BssECI	CCNNGG	5 cut(s)	579, 830, 935, 1425, 1695
BssMI	GATC	8 cut(s)	51, 301, 344, 607, 1205, 1244, 1252, 1432
BssNI	GRCGYC	1 cut(s)	60
BssT1I	CCWWGG	3 cut(s)	935, 1425, 1695
Bst2UI	CCWGG	4 cut(s)	831, 983, 1727, 1752
Bst6I	CTCTTC	3 cut(s)	77, 235, 1838
BstACI	GRCGYC	1 cut(s)	60
BstAPI	GCANNNNNTGC	2 cut(s)	377, 1828
BstBAI	YACGTR	1 cut(s)	1404
BstBI	TTCGAA	1 cut(s)	1074
BstC8I	GCNNGC	3 cut(s)	196, 719, 723
BstDEI	CTNAG	7 cut(s)	726, 918, 1040, 1171, 1234, 1553, 1716
BstEII	GGTNACC	1 cut(s)	1661
BstF5I	GGATG	3 cut(s)	949, 1035, 1418
BstFNI	CGCG	1 cut(s)	632
BstH2I	RGCGCY	2 cut(s)	606, 982
BstHHI	GCGC	2 cut(s)	605, 981
BstKTI	GATC	8 cut(s)	54, 304, 347, 610, 1208, 1247, 1255, 1435
BstMAI	GTCTC	3 cut(s)	1029, 1167, 1517
BstMBI	GATC	8 cut(s)	51, 301, 344, 607, 1205, 1244, 1252, 1432
BstMCI	CGRYCG	1 cut(s)	591
BstNI	CCWGG	4 cut(s)	831, 983, 1727, 1752
BstPI	GGTNACC	1 cut(s)	1661
BstSCI	CCNGG	6 cut(s)	579, 590, 829, 981, 1725, 1750
BstSFI	CTRYAG	1 cut(s)	1732
BstUI	CGCG	1 cut(s)	632
BstV1I	GCAGC	7 cut(s)	31, 34, 307, 383, 1746, 1831, 1834
BstV2I	GAAGAC	1 cut(s)	1444
BstX2I	RGATCY	2 cut(s)	344, 1244
BstXI	CCANNNNNNTGG	4 cut(s)	1100, 1358, 1484, 1576
BstYI	RGATCY	2 cut(s)	344, 1244
BstZI	CGGCCG	1 cut(s)	588
Bsu36I	CCTNAGG	1 cut(s)	918
BsuI	GTATCC	1 cut(s)	825
BsuRI	GGCC	4 cut(s)	533, 590, 1509, 1558
BtgZI	GCGATG	1 cut(s)	118
BtsCI	GGATG	3 cut(s)	949, 1035, 1418
BtsI	GCAGTG	1 cut(s)	1499
BtsIMutI	CAGTG	6 cut(s)	96, 381, 1294, 1489, 1499, 1525
BveI	ACCTGC	1 cut(s)	1836
Cac8I	GCNNGC	3 cut(s)	196, 719, 723
CaiI	CAGNNNCTG	2 cut(s)	377, 1828
CciI	TCATGA	2 cut(s)	150, 1306
CfoI	GCGC	2 cut(s)	605, 981
Cfr10I	RCCGGY	1 cut(s)	721
CseI	GACGC	1 cut(s)	1772
Csp6I	GTAC	5 cut(s)	276, 465, 1561, 1580, 1807
CviAII	CATG	8 cut(s)	121, 151, 788, 1307, 1352, 1603, 1613, 1767
CviQI	GTAC	5 cut(s)	276, 465, 1561, 1580, 1807
DdeI	CTNAG	7 cut(s)	726, 918, 1040, 1171, 1234, 1553, 1716
DpnI	GATC	8 cut(s)	53, 303, 346, 609, 1207, 1246, 1254, 1434
DpnII	GATC	8 cut(s)	51, 301, 344, 607, 1205, 1244, 1252, 1432
DriI	GACNNNNNGTC	1 cut(s)	159
EaeI	YGGCCR	2 cut(s)	588, 1507
EagI	CGGCCG	1 cut(s)	588
Eam1104I	CTCTTC	3 cut(s)	77, 235, 1838
Eam1105I	GACNNNNNGTC	1 cut(s)	159
EarI	CTCTTC	3 cut(s)	77, 235, 1838
EciI	GGCGGA	3 cut(s)	57, 279, 312
Ecl136II	GAGCTC	1 cut(s)	1792
EclXI	CGGCCG	1 cut(s)	588
Eco130I	CCWWGG	3 cut(s)	935, 1425, 1695
Eco24I	GRGCYC	1 cut(s)	1794
Eco52I	CGGCCG	1 cut(s)	588
Eco53kI	GAGCTC	1 cut(s)	1792
Eco57I	CTGAAG	1 cut(s)	142
Eco81I	CCTNAGG	1 cut(s)	918
Eco88I	CYCGRG	1 cut(s)	169
Eco91I	GGTNACC	1 cut(s)	1661
EcoICRI	GAGCTC	1 cut(s)	1792
EcoO65I	GGTNACC	1 cut(s)	1661
EcoRI	GAATTC	2 cut(s)	709, 1498
EcoRII	CCWGG	4 cut(s)	829, 981, 1725, 1750
EcoT14I	CCWWGG	3 cut(s)	935, 1425, 1695
EcoT38I	GRGCYC	1 cut(s)	1794
ErhI	CCWWGG	3 cut(s)	935, 1425, 1695
FaeI	CATG	8 cut(s)	124, 154, 791, 1310, 1355, 1606, 1616, 1770
FaqI	GGGAC	1 cut(s)	147
FatI	CATG	8 cut(s)	120, 150, 787, 1306, 1351, 1602, 1612, 1766
FauI	CCCGC	2 cut(s)	376, 1572
FauNDI	CATATG	2 cut(s)	112, 954
FokI	GGATG	3 cut(s)	956, 1022, 1425
FriOI	GRGCYC	1 cut(s)	1794
FspBI	CTAG	2 cut(s)	342, 1796
GlaI	GCGC	2 cut(s)	604, 980
GsuI	CTGGAG	2 cut(s)	429, 1604
HaeII	RGCGCY	2 cut(s)	606, 982
HaeIII	GGCC	4 cut(s)	533, 590, 1509, 1558
HgaI	GACGC	1 cut(s)	1772
HhaI	GCGC	2 cut(s)	605, 981
Hin1I	GRCGYC	1 cut(s)	60
Hin1II	CATG	8 cut(s)	124, 154, 791, 1310, 1355, 1606, 1616, 1770
Hin6I	GCGC	2 cut(s)	603, 979
HinP1I	GCGC	2 cut(s)	603, 979
HincII	GTYRAC	1 cut(s)	64
HindII	GTYRAC	1 cut(s)	64
HindIII	AAGCTT	2 cut(s)	1107, 1392
HinfI	GANTC	5 cut(s)	415, 809, 998, 1022, 1303
HphI	GGTGA	8 cut(s)	465, 769, 811, 1007, 1063, 1373, 1655, 1704
Hpy166II	GTNNAC	3 cut(s)	64, 248, 1302
Hpy188III	TCNNGA	9 cut(s)	13, 151, 342, 1178, 1229, 1307, 1376, 1651, 1760
Hpy8I	GTNNAC	3 cut(s)	64, 248, 1302
HpyAV	CCTTC	9 cut(s)	166, 268, 796, 1129, 1177, 1337, 1582, 1648, 1757
HpyCH4IV	ACGT	3 cut(s)	60, 429, 1403
HpyCH4V	TGCA	8 cut(s)	110, 194, 647, 927, 1035, 1143, 1734, 1831
HpyF3I	CTNAG	7 cut(s)	726, 918, 1040, 1171, 1234, 1553, 1716
HpySE526I	ACGT	3 cut(s)	60, 429, 1403
Hsp92I	GRCGYC	1 cut(s)	60
Hsp92II	CATG	8 cut(s)	124, 154, 791, 1310, 1355, 1606, 1616, 1770
HspAI	GCGC	2 cut(s)	603, 979
Kpn2I	TCCGGA	1 cut(s)	1650
KroI	GCCGGC	1 cut(s)	721
KroNI	GCCGGC	1 cut(s)	723
Kzo9I	GATC	8 cut(s)	51, 301, 344, 607, 1205, 1244, 1252, 1432
LmnI	GCTCC	2 cut(s)	682, 1797
Lsp1109I	GCAGC	7 cut(s)	31, 34, 307, 383, 1746, 1831, 1834
LweI	GCATC	6 cut(s)	126, 383, 1044, 1255, 1411, 1563
MaeI	CTAG	2 cut(s)	342, 1796
MaeII	ACGT	3 cut(s)	60, 429, 1403
MaeIII	GTNAC	6 cut(s)	10, 448, 783, 1514, 1615, 1661
MalI	GATC	8 cut(s)	53, 303, 346, 609, 1207, 1246, 1254, 1434
MboI	GATC	8 cut(s)	51, 301, 344, 607, 1205, 1244, 1252, 1432
MfeI	CAATTG	1 cut(s)	862
MflI	RGATCY	2 cut(s)	344, 1244
MhlI	GDGCHC	1 cut(s)	1794
MlsI	TGGCCA	1 cut(s)	1509
MluCI	AATT	9 cut(s)	214, 520, 667, 709, 772, 862, 902, 1364, 1498
MluNI	TGGCCA	1 cut(s)	1509
MlyI	GAGTC	2 cut(s)	1031, 1297
MmeI	TCCRAC	2 cut(s)	921, 939
Mox20I	TGGCCA	1 cut(s)	1509
MroI	TCCGGA	1 cut(s)	1650
MroNI	GCCGGC	1 cut(s)	721
MroXI	GAANNNNTTC	1 cut(s)	1221
MscI	TGGCCA	1 cut(s)	1509
MseI	TTAA	2 cut(s)	671, 1397
MslI	CAYNNNNRTG	4 cut(s)	1098, 1356, 1482, 1637
Msp20I	TGGCCA	1 cut(s)	1509
MspR9I	CCNGG	6 cut(s)	581, 592, 831, 983, 1727, 1752
MunI	CAATTG	1 cut(s)	862
MvaI	CCWGG	4 cut(s)	831, 983, 1727, 1752
MvnI	CGCG	1 cut(s)	632
NaeI	GCCGGC	1 cut(s)	723
NciI	CCSGG	2 cut(s)	581, 592
NdeI	CATATG	2 cut(s)	112, 954
NdeII	GATC	8 cut(s)	51, 301, 344, 607, 1205, 1244, 1252, 1432
NgoMIV	GCCGGC	1 cut(s)	721
NlaIII	CATG	8 cut(s)	124, 154, 791, 1310, 1355, 1606, 1616, 1770
NlaIV	GGNNCC	3 cut(s)	562, 615, 1745
NmeAIII	GCCGAG	1 cut(s)	1813
NmuCI	GTSAC	5 cut(s)	10, 448, 1514, 1615, 1661
NspV	TTCGAA	1 cut(s)	1074
PagI	TCATGA	2 cut(s)	150, 1306
PaqCI	CACCTGC	1 cut(s)	1836
PcsI	WCGNNNNNNNCGW	1 cut(s)	629
PdiI	GCCGGC	1 cut(s)	723
PdmI	GAANNNNTTC	1 cut(s)	1221
PfeI	GAWTC	3 cut(s)	415, 809, 998
PfoI	TCCNGGA	1 cut(s)	1725
PleI	GAGTC	2 cut(s)	1030, 1297
PpsI	GAGTC	2 cut(s)	1030, 1297
Ppu21I	YACGTR	1 cut(s)	1404
Psp124BI	GAGCTC	1 cut(s)	1794
Psp6I	CCWGG	4 cut(s)	829, 981, 1725, 1750
PspEI	GGTNACC	1 cut(s)	1661
PspGI	CCWGG	4 cut(s)	829, 981, 1725, 1750
PspN4I	GGNNCC	3 cut(s)	562, 615, 1745
PstI	CTGCAG	1 cut(s)	1736
PstNI	CAGNNNCTG	2 cut(s)	377, 1828
PsuI	RGATCY	2 cut(s)	344, 1244
RsaI	GTAC	5 cut(s)	277, 466, 1562, 1581, 1808
RsaNI	GTAC	5 cut(s)	276, 465, 1561, 1580, 1807
RseI	CAYNNNNRTG	4 cut(s)	1098, 1356, 1482, 1637
SacI	GAGCTC	1 cut(s)	1794
SaqAI	TTAA	2 cut(s)	671, 1397
Sau3AI	GATC	8 cut(s)	51, 301, 344, 607, 1205, 1244, 1252, 1432
ScaI	AGTACT	2 cut(s)	277, 466
SchI	GAGTC	2 cut(s)	1031, 1297
ScrFI	CCNGG	6 cut(s)	581, 592, 831, 983, 1727, 1752
SduI	GDGCHC	1 cut(s)	1794
SfaNI	GCATC	6 cut(s)	126, 383, 1044, 1255, 1411, 1563
SfcI	CTRYAG	1 cut(s)	1732
SfuI	TTCGAA	1 cut(s)	1074
SmiMI	CAYNNNNRTG	4 cut(s)	1098, 1356, 1482, 1637
SmlI	CTYRAG	1 cut(s)	1376
SmoI	CTYRAG	1 cut(s)	1376
Sse9I	AATT	9 cut(s)	214, 520, 667, 709, 772, 862, 902, 1364, 1498
SspMI	CTAG	2 cut(s)	342, 1796
SstI	GAGCTC	1 cut(s)	1794
StyD4I	CCNGG	6 cut(s)	579, 590, 829, 981, 1725, 1750
StyI	CCWWGG	3 cut(s)	935, 1425, 1695
TaiI	ACGT	3 cut(s)	63, 432, 1406
TaqI	TCGA	5 cut(s)	239, 304, 623, 1074, 1177
TaqII	GACCGA	1 cut(s)	521
TasI	AATT	9 cut(s)	214, 520, 667, 709, 772, 862, 902, 1364, 1498
TatI	WGTACW	2 cut(s)	275, 464
TauI	GCSGC	4 cut(s)	295, 328, 365, 590
TfiI	GAWTC	3 cut(s)	415, 809, 998
Tru1I	TTAA	2 cut(s)	671, 1397
Tru9I	TTAA	2 cut(s)	671, 1397
TscAI	CASTG	6 cut(s)	103, 388, 1301, 1489, 1499, 1525
TseFI	GTSAC	5 cut(s)	10, 448, 1514, 1615, 1661
TseI	GCWGC	7 cut(s)	19, 22, 295, 371, 1734, 1819, 1822
Tsp45I	GTSAC	5 cut(s)	10, 448, 1514, 1615, 1661
TspDTI	ATGAA	8 cut(s)	121, 139, 273, 717, 785, 867, 1111, 1619
TspRI	CASTG	6 cut(s)	103, 388, 1301, 1489, 1499, 1525
XapI	RAATTY	5 cut(s)	214, 667, 709, 902, 1498
XbaI	TCTAGA	1 cut(s)	341
XcmI	CCANNNNNNNNNTGG	2 cut(s)	97, 1484
XmnI	GAANNNNTTC	1 cut(s)	1221
XspI	CTAG	2 cut(s)	342, 1796
ZraI	GACGTC	1 cut(s)	61
ZrmI	AGTACT	2 cut(s)	277, 466

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.