MD14G1067500.v1.1

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0009290
Physical Location & Seq
Forward (+)
7350392 .. 7355251
4860 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1067500.v1.1.491

Sequence Viewer

Length: 1218 bp
ATGGTCAAGGAAACGGAGTACTACGACGTCCTCGGCGTCAGCCCGTCCGCCACCGAGGCGGAGATCAAGAAAGCTTATTACATGAAGGCAAGGCAGGTTCATCCAGATAAAAACCCAAATGATCCACTTGCAGCACAAAATTTTCAGGTTTTGGGAGAAGCTTACCAAGTGTTAAGCGACCCAAGCCAACGCCAAGCATATGATGCTAATGGAAAATCAGGGATTTCAACCGACGCAATTATTGATCCTGCAGCGATCTTCGCCATGCTTTTTGGTAGTGAGCTTTTCGAGGAATATATTGGACAGCTAGCCATGGCATCAATGGCTTCCCTAGATATTTTCACAGAAGGCGAACAGTTTGATCCCAAAAAGTTGCAAGAGAAAATGCGGGTTGTTCAAAAGGAAAGAGAAGAAAAGCTTGCAGAAACACTAAAAAATCGACTCAACCAATATGTGCAAGGCAACAAAGAGGAATTTGTTAGTCATGCTGAAGCTGAAGTTGCGAGGCTTTCCAATGCAGCCTATGGTGTTGATATGTTGAATACGATTGGCTACATATATGCCAGACAATCAGCAAAGGAGCTAGGCAAGAAAGCAATATATTTGGGCGTGCCATTTGTTGCAGAGTGGTTTAGAAATAAAGGGCATTTCATTAAATCCCAAGTAACAGCAGCAACAGGTGCAATTGCTTTGCTCCAGCTGCAGGAAGACATGAAAAAGCAGCTAAGTGCAGAAGGAAATTACACAGAGGAAGAGCTCGAAGAATACATGCAATCTCACAAGAAGTTGATGATTGATTCCCTGTGGAAGCTTAATGTGGCTGATATCGAGGCCACTCTTTCTCGTGTTTGTCAGATGGTTCTTCAAGACAGCAATGCCAAGAAAGAGGAACTCCGTGCGCGAGCAAAGGGGTTGAAAACTCTTGGCAGAATCTTCCAGAGGACCAAGTCAACCGGTGGGAATGAGGGTGAACCTGCACAAAATAGTAGAGTCCATAAACTGAATGGAAGTGAATCAAGCTATGATACCTCGTCTCCTATAACAACACCAAAGTCCTCAAATCTGGAAGAGCCATCTTACACAGCATTTGAGCCACAGGTGTCGCAGAGCCCCTATGTGGAAGCCCCCCAAATTGCCGGTGAACAGTTGAACTATCACTTCCCAAGGCCAACTGCACCACCTGGTGCCCAGAGACATTCTTCCATTGGCAGGCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ-X

Protein Analysis

406

Amino Acids

45.21

Weight (kDa)

5.77

Isoelectric Point (pI)

49.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 6 - 68 1.1e-25 DnaJ domain
DnaJ-X PF14308 133 - 326 5.2e-56 X-domain of DnaJ-containing
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 30
Acc36I ACCTGC 2 cut(s) 85, 982
AccB1I GGYRCC 1 cut(s) 1184
AccII CGCG 1 cut(s) 901
AciI CCGC 3 cut(s) 48, 59, 388
AclWI GGATC 3 cut(s) 116, 239, 356
AcsI RAATTY 2 cut(s) 139, 473
AcuI CTGAAG 2 cut(s) 510, 516
AcyI GRCGYC 2 cut(s) 27, 36
AdeI CACNNNGTG 1 cut(s) 1184
AfaI GTAC 1 cut(s) 20
AfiI CCNNNNNNNGG 3 cut(s) 703, 1117, 1209
AgeI ACCGGT 1 cut(s) 953
AgsI TTSAA 6 cut(s) 228, 398, 541, 866, 916, 1150
AjnI CCWGG 1 cut(s) 1180
Alw21I GWGCWC 1 cut(s) 759
Alw26I GTCTC 2 cut(s) 1038, 1186
AlwI GGATC 3 cut(s) 116, 239, 356
AoxI GGCC 2 cut(s) 831, 1166
ApeKI GCWGC 6 cut(s) 131, 251, 518, 671, 700, 721
ApoI RAATTY 2 cut(s) 139, 473
AsiGI ACCGGT 1 cut(s) 953
AspLEI GCGC 1 cut(s) 901
AspS9I GGNCC 1 cut(s) 942
AsuHPI GGTGA 2 cut(s) 980, 1151
AsuNHI GCTAGC 1 cut(s) 307
AvaII GGWCC 1 cut(s) 942
BaeGI GKGCMC 1 cut(s) 1189
BanI GGYRCC 1 cut(s) 1184
BanII GRGCYC 2 cut(s) 759, 1112
BauI CACGAG 1 cut(s) 843
BbsI GAAGAC 1 cut(s) 714
Bbv12I GWGCWC 1 cut(s) 759
BbvI GCAGC 6 cut(s) 143, 263, 530, 683, 687, 733
BccI CCATC 2 cut(s) 850, 1081
BciT130I CCWGG 1 cut(s) 1182
BcoDI GTCTC 2 cut(s) 1038, 1186
BfaI CTAG 3 cut(s) 308, 332, 584
BfmI CTRYAG 2 cut(s) 249, 701
BfuAI ACCTGC 2 cut(s) 85, 982
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 6 cut(s) 132, 252, 519, 672, 701, 722
BlsI GCNGC 6 cut(s) 133, 253, 520, 673, 702, 723
BmcAI AGTACT 1 cut(s) 20
Bme1390I CCNGG 1 cut(s) 1182
Bme18I GGWCC 1 cut(s) 942
BmgT120I GGNCC 1 cut(s) 942
BmiI GGNNCC 1 cut(s) 1186
BmrFI CCNGG 1 cut(s) 1182
BmsI GCATC 2 cut(s) 193, 326
BmtI GCTAGC 1 cut(s) 311
BpiI GAAGAC 1 cut(s) 714
BpmI CTGGAG 1 cut(s) 680
BsaHI GRCGYC 2 cut(s) 27, 36
BsaJI CCNNGG 4 cut(s) 31, 54, 312, 1163
BsaWI WCCGGW 1 cut(s) 953
BsaXI ACNNNNNCTCC 2 cut(s) 1018, 1048
Bsc4I CCNNNNNNNGG 3 cut(s) 703, 1117, 1209
Bse118I RCCGGY 2 cut(s) 953, 1136
Bse3DI GCAATG 1 cut(s) 880
BseBI CCWGG 1 cut(s) 1182
BseDI CCNNGG 4 cut(s) 31, 54, 312, 1163
BseGI GGATG 1 cut(s) 100
BseLI CCNNNNNNNGG 3 cut(s) 703, 1117, 1209
BseMI GCAATG 1 cut(s) 880
BseSI GKGCMC 1 cut(s) 1189
BseXI GCAGC 6 cut(s) 143, 263, 530, 683, 687, 733
BsgI GTGCAG 3 cut(s) 750, 960, 1158
Bsh1236I CGCG 1 cut(s) 901
BshFI GGCC 2 cut(s) 833, 1168
BshNI GGYRCC 1 cut(s) 1184
BshTI ACCGGT 1 cut(s) 953
BsiHKAI GWGCWC 1 cut(s) 759
BsiSI CCGG 2 cut(s) 954, 1137
BslI CCNNNNNNNGG 3 cut(s) 703, 1117, 1209
BsmAI GTCTC 2 cut(s) 1038, 1186
BsmBI CGTCTC 1 cut(s) 1038
BsnI GGCC 2 cut(s) 833, 1168
Bsp1286I GDGCHC 3 cut(s) 759, 1112, 1189
Bsp143I GATC 5 cut(s) 63, 121, 244, 255, 361
Bsp19I CCATGG 1 cut(s) 312
BspACI CCGC 3 cut(s) 48, 59, 388
BspANI GGCC 2 cut(s) 833, 1168
BspFNI CGCG 1 cut(s) 901
BspLI GGNNCC 1 cut(s) 1186
BspMAI CTGCAG 2 cut(s) 253, 705
BspMI ACCTGC 2 cut(s) 85, 982
BspOI GCTAGC 1 cut(s) 311
BspPI GGATC 3 cut(s) 116, 239, 356
BspQI GCTCTTC 2 cut(s) 747, 1062
BspT107I GGYRCC 1 cut(s) 1184
BsrDI GCAATG 1 cut(s) 880
BsrFI RCCGGY 2 cut(s) 953, 1136
BssAI RCCGGY 2 cut(s) 953, 1136
BssECI CCNNGG 4 cut(s) 31, 54, 312, 1163
BssMI GATC 5 cut(s) 63, 121, 244, 255, 361
BssNI GRCGYC 2 cut(s) 27, 36
BssSI CACGAG 1 cut(s) 843
BssT1I CCWWGG 2 cut(s) 312, 1163
Bst2BI CACGAG 1 cut(s) 843
Bst2UI CCWGG 1 cut(s) 1182
Bst4CI ACNGT 2 cut(s) 357, 1146
Bst6I CTCTTC 2 cut(s) 747, 1062
BstACI GRCGYC 2 cut(s) 27, 36
BstAPI GCANNNNNTGC 2 cut(s) 203, 680
BstC8I GCNNGC 5 cut(s) 309, 420, 611, 903, 1211
BstDEI CTNAG 1 cut(s) 725
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 100
BstFNI CGCG 1 cut(s) 901
BstHHI GCGC 1 cut(s) 901
BstKTI GATC 5 cut(s) 66, 124, 247, 258, 364
BstMAI GTCTC 2 cut(s) 1038, 1186
BstMBI GATC 5 cut(s) 63, 121, 244, 255, 361
BstMWI GCNNNNNNNGC 8 cut(s) 56, 183, 203, 260, 323, 500, 680, 700
BstNI CCWGG 1 cut(s) 1182
BstNSI RCATGY 1 cut(s) 772
BstSCI CCNGG 1 cut(s) 1180
BstSFI CTRYAG 2 cut(s) 249, 701
BstSLI GKGCMC 1 cut(s) 1189
BstUI CGCG 1 cut(s) 901
BstV1I GCAGC 6 cut(s) 143, 263, 530, 683, 687, 733
BstV2I GAAGAC 1 cut(s) 714
BsuRI GGCC 2 cut(s) 833, 1168
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 1 cut(s) 100
BveI ACCTGC 2 cut(s) 85, 982
Cac8I GCNNGC 5 cut(s) 309, 420, 611, 903, 1211
CfoI GCGC 1 cut(s) 901
Cfr10I RCCGGY 2 cut(s) 953, 1136
Cfr13I GGNCC 1 cut(s) 942
CseI GACGC 2 cut(s) 25, 242
CsiI ACCWGGT 1 cut(s) 1180
Csp6I GTAC 1 cut(s) 19
CspAI ACCGGT 1 cut(s) 953
CviAII CATG 6 cut(s) 82, 265, 313, 485, 712, 769
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 1 cut(s) 725
DpnI GATC 5 cut(s) 65, 123, 246, 257, 363
DpnII GATC 5 cut(s) 63, 121, 244, 255, 361
DraIII CACNNNGTG 1 cut(s) 1184
Eam1104I CTCTTC 2 cut(s) 747, 1062
EarI CTCTTC 2 cut(s) 747, 1062
EciI GGCGGA 2 cut(s) 37, 74
Ecl136II GAGCTC 1 cut(s) 757
Eco130I CCWWGG 2 cut(s) 312, 1163
Eco24I GRGCYC 2 cut(s) 759, 1112
Eco32I GATATC 1 cut(s) 826
Eco47I GGWCC 1 cut(s) 942
Eco53kI GAGCTC 1 cut(s) 757
Eco57I CTGAAG 2 cut(s) 510, 516
EcoICRI GAGCTC 1 cut(s) 757
EcoRII CCWGG 1 cut(s) 1180
EcoRV GATATC 1 cut(s) 826
EcoT14I CCWWGG 2 cut(s) 312, 1163
EcoT38I GRGCYC 2 cut(s) 759, 1112
ErhI CCWWGG 2 cut(s) 312, 1163
Esp3I CGTCTC 1 cut(s) 1038
FaeI CATG 6 cut(s) 85, 268, 316, 488, 715, 772
FalI AAGNNNNNCTT 2 cut(s) 402, 434
FatI CATG 6 cut(s) 81, 264, 312, 484, 711, 768
FauI CCCGC 1 cut(s) 381
FauNDI CATATG 1 cut(s) 199
Fnu4HI GCNGC 6 cut(s) 132, 252, 519, 672, 701, 722
FokI GGATG 1 cut(s) 87
FriOI GRGCYC 2 cut(s) 759, 1112
Fsp4HI GCNGC 6 cut(s) 132, 252, 519, 672, 701, 722
FspBI CTAG 3 cut(s) 308, 332, 584
GlaI GCGC 1 cut(s) 900
GluI GCNGC 6 cut(s) 132, 252, 519, 672, 701, 722
GsuI CTGGAG 1 cut(s) 680
HaeIII GGCC 2 cut(s) 833, 1168
HapII CCGG 2 cut(s) 954, 1137
HgaI GACGC 2 cut(s) 25, 242
HhaI GCGC 1 cut(s) 901
Hin1I GRCGYC 2 cut(s) 27, 36
Hin1II CATG 6 cut(s) 85, 268, 316, 488, 715, 772
Hin6I GCGC 1 cut(s) 899
HinP1I GCGC 1 cut(s) 899
HincII GTYRAC 1 cut(s) 951
HindII GTYRAC 1 cut(s) 951
HindIII AAGCTT 4 cut(s) 72, 159, 416, 809
HinfI GANTC 5 cut(s) 441, 797, 930, 990, 1013
HpaII CCGG 2 cut(s) 954, 1137
HphI GGTGA 2 cut(s) 980, 1151
Hpy166II GTNNAC 3 cut(s) 951, 971, 1142
Hpy188I TCNGA 1 cut(s) 855
Hpy188III TCNNGA 5 cut(s) 67, 104, 866, 937, 1064
Hpy8I GTNNAC 3 cut(s) 951, 971, 1142
Hpy99I CGWCG 2 cut(s) 29, 236
HpyAV CCTTC 3 cut(s) 79, 341, 728
HpyCH4III ACNGT 2 cut(s) 357, 1146
HpyCH4IV ACGT 1 cut(s) 27
HpyF10VI GCNNNNNNNGC 8 cut(s) 56, 183, 203, 260, 323, 500, 680, 700
HpyF3I CTNAG 1 cut(s) 725
HpySE526I ACGT 1 cut(s) 27
Hsp92I GRCGYC 2 cut(s) 27, 36
Hsp92II CATG 6 cut(s) 85, 268, 316, 488, 715, 772
HspAI GCGC 1 cut(s) 899
Kzo9I GATC 5 cut(s) 63, 121, 244, 255, 361
LguI GCTCTTC 2 cut(s) 747, 1062
LmnI GCTCC 2 cut(s) 580, 699
Lsp1109I GCAGC 6 cut(s) 143, 263, 530, 683, 687, 733
LweI GCATC 2 cut(s) 193, 326
MabI ACCWGGT 1 cut(s) 1180
MaeI CTAG 3 cut(s) 308, 332, 584
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 664
MalI GATC 5 cut(s) 65, 123, 246, 257, 363
MboI GATC 5 cut(s) 63, 121, 244, 255, 361
MboII GAAGA 9 cut(s) 250, 422, 719, 764, 773, 854, 925, 1079, 1191
MfeI CAATTG 1 cut(s) 684
MhlI GDGCHC 3 cut(s) 759, 1112, 1189
MluCI AATT 6 cut(s) 139, 237, 473, 684, 739, 1131
MlyI GAGTC 2 cut(s) 435, 999
MseI TTAA 3 cut(s) 173, 654, 813
MspA1I CMGCKG 1 cut(s) 700
MspI CCGG 2 cut(s) 954, 1137
MspR9I CCNGG 1 cut(s) 1182
MunI CAATTG 1 cut(s) 684
MvaI CCWGG 1 cut(s) 1182
MvnI CGCG 1 cut(s) 901
MwoI GCNNNNNNNGC 8 cut(s) 56, 183, 203, 260, 323, 500, 680, 700
NcoI CCATGG 1 cut(s) 312
NdeI CATATG 1 cut(s) 199
NdeII GATC 5 cut(s) 63, 121, 244, 255, 361
NheI GCTAGC 1 cut(s) 307
NlaIII CATG 6 cut(s) 85, 268, 316, 488, 715, 772
NlaIV GGNNCC 1 cut(s) 1186
NmeAIII GCCGAG 1 cut(s) 12
NspI RCATGY 1 cut(s) 772
PciSI GCTCTTC 2 cut(s) 747, 1062
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PfeI GAWTC 3 cut(s) 797, 930, 1013
PflFI GACNNNGTC 1 cut(s) 946
PinAI ACCGGT 1 cut(s) 953
PkrI GCNGC 6 cut(s) 133, 253, 520, 673, 702, 723
PleI GAGTC 2 cut(s) 435, 998
PpsI GAGTC 2 cut(s) 435, 998
Psp124BI GAGCTC 1 cut(s) 759
Psp6I CCWGG 1 cut(s) 1180
PspGI CCWGG 1 cut(s) 1180
PspN4I GGNNCC 1 cut(s) 1186
PspPI GGNCC 1 cut(s) 942
PstI CTGCAG 2 cut(s) 253, 705
PsyI GACNNNGTC 1 cut(s) 946
PvuII CAGCTG 1 cut(s) 700
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
SacI GAGCTC 1 cut(s) 759
SapI GCTCTTC 2 cut(s) 747, 1062
SaqAI TTAA 3 cut(s) 173, 654, 813
SatI GCNGC 6 cut(s) 132, 252, 519, 672, 701, 722
Sau3AI GATC 5 cut(s) 63, 121, 244, 255, 361
Sau96I GGNCC 1 cut(s) 942
ScaI AGTACT 1 cut(s) 20
SchI GAGTC 2 cut(s) 435, 999
ScrFI CCNGG 1 cut(s) 1182
SduI GDGCHC 3 cut(s) 759, 1112, 1189
SexAI ACCWGGT 1 cut(s) 1180
SfaNI GCATC 2 cut(s) 193, 326
SfcI CTRYAG 2 cut(s) 249, 701
SinI GGWCC 1 cut(s) 942
Sse9I AATT 6 cut(s) 139, 237, 473, 684, 739, 1131
SsiI CCGC 3 cut(s) 48, 59, 388
SspMI CTAG 3 cut(s) 308, 332, 584
SstI GAGCTC 1 cut(s) 759
StyD4I CCNGG 1 cut(s) 1180
StyI CCWWGG 2 cut(s) 312, 1163
TaaI ACNGT 2 cut(s) 357, 1146
TaiI ACGT 1 cut(s) 30
TaqI TCGA 4 cut(s) 288, 439, 759, 828
TasI AATT 6 cut(s) 139, 237, 473, 684, 739, 1131
TatI WGTACW 1 cut(s) 18
TfiI GAWTC 3 cut(s) 797, 930, 1013
Tru1I TTAA 3 cut(s) 173, 654, 813
Tru9I TTAA 3 cut(s) 173, 654, 813
TseI GCWGC 6 cut(s) 131, 251, 518, 671, 700, 721
TspDTI ATGAA 4 cut(s) 89, 98, 640, 728
TspGWI ACGGA 2 cut(s) 29, 884
Tth111I GACNNNGTC 1 cut(s) 946
VpaK11BI GGWCC 1 cut(s) 942
XapI RAATTY 2 cut(s) 139, 473
XceI RCATGY 1 cut(s) 772
XcmI CCANNNNNNNNNTGG 2 cut(s) 319, 1001
XspI CTAG 3 cut(s) 308, 332, 584
ZraI GACGTC 1 cut(s) 28
ZrmI AGTACT 1 cut(s) 20
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.