Rorug07G0172300

Chaperone protein DNAj

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0009290
Physical Location & Seq
Forward (+)
14117399 .. 14121099
3701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0172300.1

Sequence Viewer

Length: 1410 bp
ATGGTGAAAGTCAAAAGGGAACAAATACACGAGGCTGTTGTGCATTCAGACAGGAAATTTGGGGTCGAGTCGTGGCCATTATCTGAAGGCAATTGTGACGCTCCTGTTTCGGAGCCAAGCCCTTTTAGTTGGAGAACGAGTGGTCCAACCAGGCGATCATCAAGATGCTGGACAGAGAAAGAGGATAAACTTCTGGTAGAGCTGGTCAGAAAGTTTGACGGGAGTAATTGGAAGCAAATAGCTGCGGGTATCCCTGGCCGTTCAGATGTTCAGTGCTTGCATCGCTGGCAAAAAGTTTTGAATCCTGAAATCATCAAGGGATACTGGACAAAGGAGGAAGATGATTGTATAATCAAGCTAGTTGAAAAATATGGTGCTAGAAGATGGTCTGTCATAGCAAAGTTTTTACCAGGTCGAATAGGCAAGCAATGCAGAGAAAGGTGGTACAATCATTTAAGCCCGGCTATAAACAAAGATGCATGGACAGAGGAAGAGGAAAGAATTCTTGCTTTCTACCACCAACAGTATGGTAACAAGTGGGCAGAAATAGCAAGGTTTCTACCTGGAAGGACTGACAATGCAATTAAAAATTACTGGAACGGCGCATTCAAGAGGAAAGAAAATTCATGCTCTCCTAATGGTAATGAGTGGGACATGCATCCTGATATCTGTATTGATGCGCTAAGATCAGATCTTGTAGGGGTTAAAGCAGTCAGTCAGAAGTTTGATAATGCTTGTTCTACAGAGTTGACTCTCGGAACGTCATATGGTTTTGAAGGAAAATCTCTGAAATCACAGATATGCGGGTCTTCAAGAAGAGGGTCAAGTGGTTTAATCACTTTGCACACCACGGTACAGTCTGATGACAGCAATGTTGCTACTCCTCTTGGGGTCAAAAGTGGATCTTGCAACCAAATTGCTGATAATACAGGCAAATACCGCCCCTTTGCACACTCTCCATTGGTTTTCATGGATTCATCCACTTCCACCAACTCAAACTGTACTCCTCCAAAGATTCCACTTTGCTATTCAACTCCACCCAACAGTGTGCAGAATGTCTATGCTAGCAGCACCAGTCCAAAATCTAGATTGAAGAGCTTAGCAAGGACTTTCAAAAATACTCCTTCCATCATAAGAAAGAGAGCTTCCAGAACCGCAGATCATGCCAACTTTTCTGGTGCCAGCTGCTCAACTGCATTGAACATCCTGAGTCTTGATGAAGCAGCTTTCAATAGCAGAGACTCCAACTCTTCAGACTATGTCAGAGGTTTATCTTCAATGCGGCATAGACCCGAAACTTCAGATGCTTATAAATCTCTTGGGAGGTGCCTGGAACATGAATTTGACGATGAAAATGACTCGGGTATCGCTAACTGTGGCAAAGTAGTCTCTGCATATGTTGGTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ DnaJ-X

Protein Analysis

469

Amino Acids

52.36

Weight (kDa)

8.9

Isoelectric Point (pI)

49.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 56 - 100 6.9e-15 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 57 - 114 2e-14 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 106 - 152 1.7e-19 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 109 - 166 3.3e-18 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 158 - 200 4e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 161 - 207 3.5e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1311
AccB1I GGYRCC 2 cut(s) 1178, 1326
AciI CCGC 5 cut(s) 245, 804, 940, 1155, 1282
AclWI GGATC 1 cut(s) 910
AcoI YGGCCR 2 cut(s) 74, 256
AcsI RAATTY 4 cut(s) 56, 501, 622, 1340
AcuI CTGAAG 3 cut(s) 105, 1236, 1284
AfaI GTAC 3 cut(s) 446, 855, 1003
AjnI CCWGG 5 cut(s) 149, 253, 409, 562, 1329
AloI GAACNNNNNNTCC 2 cut(s) 127, 159
AluBI AGCT 7 cut(s) 202, 242, 358, 1098, 1145, 1185, 1226
AluI AGCT 7 cut(s) 202, 242, 358, 1098, 1145, 1185, 1226
Alw26I GTCTC 2 cut(s) 1233, 1393
AlwI GGATC 1 cut(s) 910
Ama87I CYCGRG 1 cut(s) 1360
AoxI GGCC 2 cut(s) 74, 256
ApeKI GCWGC 4 cut(s) 242, 1068, 1185, 1223
ApoI RAATTY 4 cut(s) 56, 501, 622, 1340
ArsI GACNNNNNNTTYG 2 cut(s) 41, 73
Asp700I GAANNNNTTC 1 cut(s) 501
AspLEI GCGC 2 cut(s) 605, 682
AspS9I GGNCC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 461
AsuHPI GGTGA 1 cut(s) 16
AsuNHI GCTAGC 1 cut(s) 1064
AvaI CYCGRG 1 cut(s) 1360
AvaII GGWCC 1 cut(s) 143
BaeI ACNNNNGTAYC 2 cut(s) 1348, 1381
BalI TGGCCA 1 cut(s) 76
BanI GGYRCC 2 cut(s) 1178, 1326
BauI CACGAG 1 cut(s) 29
BbsI GAAGAC 1 cut(s) 801
BbvI GCAGC 4 cut(s) 229, 1080, 1172, 1235
BccI CCATC 2 cut(s) 378, 1136
BceAI ACGGC 2 cut(s) 243, 616
BciT130I CCWGG 5 cut(s) 151, 255, 411, 564, 1331
BciVI GTATCC 2 cut(s) 260, 314
BcnI CCSGG 1 cut(s) 461
BcoDI GTCTC 2 cut(s) 1233, 1393
BfaI CTAG 4 cut(s) 359, 378, 1065, 1086
BfmI CTRYAG 1 cut(s) 741
BfuI GTATCC 2 cut(s) 260, 314
BglII AGATCT 1 cut(s) 691
BisI GCNGC 5 cut(s) 243, 1069, 1186, 1224, 1283
BlpI GCTNAGC 1 cut(s) 1099
BlsI GCNGC 5 cut(s) 244, 1070, 1187, 1225, 1284
Bme1390I CCNGG 6 cut(s) 151, 255, 411, 461, 564, 1331
Bme18I GGWCC 1 cut(s) 143
BmeT110I CYCGRG 1 cut(s) 1360
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 3 cut(s) 114, 1180, 1328
BmrFI CCNGG 6 cut(s) 151, 255, 411, 461, 564, 1331
BmsI GCATC 6 cut(s) 155, 289, 466, 667, 667, 1294
BmtI GCTAGC 1 cut(s) 1068
BpiI GAAGAC 1 cut(s) 801
Bpu1102I GCTNAGC 1 cut(s) 1099
BpuMI CCSGG 1 cut(s) 461
BsaJI CCNNGG 2 cut(s) 253, 849
BsaXI ACNNNNNCTCC 2 cut(s) 124, 154
Bse1I ACTGG 3 cut(s) 329, 599, 1074
Bse3DI GCAATG 2 cut(s) 434, 877
BseBI CCWGG 5 cut(s) 151, 255, 411, 564, 1331
BseDI CCNNGG 2 cut(s) 253, 849
BseGI GGATG 3 cut(s) 658, 977, 1203
BseMI GCAATG 2 cut(s) 434, 877
BseMII CTCAG 1 cut(s) 1199
BseNI ACTGG 3 cut(s) 329, 599, 1074
BseRI GAGGAG 2 cut(s) 873, 996
BseXI GCAGC 4 cut(s) 229, 1080, 1172, 1235
BsgI GTGCAG 1 cut(s) 1070
BshFI GGCC 2 cut(s) 76, 258
BshNI GGYRCC 2 cut(s) 1178, 1326
BsiHKCI CYCGRG 1 cut(s) 1360
BsiSI CCGG 1 cut(s) 461
BslFI GGGAC 1 cut(s) 665
BsmAI GTCTC 2 cut(s) 1233, 1393
BsmFI GGGAC 1 cut(s) 665
BsmI GAATGC 2 cut(s) 43, 605
BsnI GGCC 2 cut(s) 76, 258
BsoBI CYCGRG 1 cut(s) 1360
Bsp143I GATC 5 cut(s) 155, 686, 691, 902, 1159
Bsp1720I GCTNAGC 1 cut(s) 1099
BspACI CCGC 5 cut(s) 245, 804, 940, 1155, 1282
BspANI GGCC 2 cut(s) 76, 258
BspCNI CTCAG 1 cut(s) 1200
BspLI GGNNCC 3 cut(s) 114, 1180, 1328
BspOI GCTAGC 1 cut(s) 1068
BspPI GGATC 1 cut(s) 910
BspQI GCTCTTC 1 cut(s) 1088
BspT107I GGYRCC 2 cut(s) 1178, 1326
BsrDI GCAATG 2 cut(s) 434, 877
BsrI ACTGG 3 cut(s) 329, 599, 1074
BssECI CCNNGG 2 cut(s) 253, 849
BssMI GATC 5 cut(s) 155, 686, 691, 902, 1159
BssSI CACGAG 1 cut(s) 29
Bst2BI CACGAG 1 cut(s) 29
Bst2UI CCWGG 5 cut(s) 151, 255, 411, 564, 1331
Bst4CI ACNGT 6 cut(s) 525, 853, 858, 1001, 1046, 1376
Bst6I CTCTTC 4 cut(s) 486, 811, 1088, 1255
BstAPI GCANNNNNTGC 2 cut(s) 429, 1163
BstC8I GCNNGC 5 cut(s) 278, 287, 425, 1066, 1183
BstDEI CTNAG 3 cut(s) 683, 1099, 1208
BstDSI CCRYGG 1 cut(s) 849
BstF5I GGATG 3 cut(s) 658, 977, 1203
BstHHI GCGC 2 cut(s) 605, 682
BstKTI GATC 5 cut(s) 158, 689, 694, 905, 1162
BstMAI GTCTC 2 cut(s) 1233, 1393
BstMBI GATC 5 cut(s) 155, 686, 691, 902, 1159
BstMWI GCNNNNNNNGC 6 cut(s) 282, 286, 429, 548, 939, 1163
BstNI CCWGG 5 cut(s) 151, 255, 411, 564, 1331
BstNSI RCATGY 1 cut(s) 658
BstSCI CCNGG 6 cut(s) 149, 253, 409, 459, 562, 1329
BstSFI CTRYAG 1 cut(s) 741
BstV1I GCAGC 4 cut(s) 229, 1080, 1172, 1235
BstV2I GAAGAC 1 cut(s) 801
BstX2I RGATCY 2 cut(s) 691, 902
BstXI CCANNNNNNTGG 1 cut(s) 527
BstYI RGATCY 2 cut(s) 691, 902
BsuI GTATCC 2 cut(s) 260, 314
BsuRI GGCC 2 cut(s) 76, 258
BtgI CCRYGG 1 cut(s) 849
BtgZI GCGATG 1 cut(s) 266
BtsCI GGATG 3 cut(s) 658, 977, 1203
BtsIMutI CAGTG 2 cut(s) 278, 1051
Cac8I GCNNGC 5 cut(s) 278, 287, 425, 1066, 1183
CfoI GCGC 2 cut(s) 605, 682
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 107
CsiI ACCWGGT 1 cut(s) 409
Csp6I GTAC 3 cut(s) 445, 854, 1002
CviAII CATG 6 cut(s) 480, 627, 655, 970, 1163, 1337
CviQI GTAC 3 cut(s) 445, 854, 1002
DdeI CTNAG 3 cut(s) 683, 1099, 1208
DpnI GATC 5 cut(s) 157, 688, 693, 904, 1161
DpnII GATC 5 cut(s) 155, 686, 691, 902, 1159
EaeI YGGCCR 2 cut(s) 74, 256
Eam1104I CTCTTC 4 cut(s) 486, 811, 1088, 1255
EarI CTCTTC 4 cut(s) 486, 811, 1088, 1255
Eco32I GATATC 1 cut(s) 667
Eco47I GGWCC 1 cut(s) 143
Eco57I CTGAAG 3 cut(s) 105, 1236, 1284
Eco88I CYCGRG 1 cut(s) 1360
EcoRI GAATTC 1 cut(s) 501
EcoRII CCWGG 5 cut(s) 149, 253, 409, 562, 1329
EcoRV GATATC 1 cut(s) 667
EcoT22I ATGCAT 2 cut(s) 481, 660
FaeI CATG 6 cut(s) 483, 630, 658, 973, 1166, 1340
FalI AAGNNNNNCTT 2 cut(s) 889, 921
FaqI GGGAC 1 cut(s) 665
FatI CATG 6 cut(s) 479, 626, 654, 969, 1162, 1336
FauI CCCGC 2 cut(s) 238, 797
FauNDI CATATG 2 cut(s) 766, 1396
Fnu4HI GCNGC 5 cut(s) 243, 1069, 1186, 1224, 1283
FokI GGATG 3 cut(s) 645, 964, 1190
Fsp4HI GCNGC 5 cut(s) 243, 1069, 1186, 1224, 1283
FspBI CTAG 4 cut(s) 359, 378, 1065, 1086
GlaI GCGC 2 cut(s) 604, 681
GluI GCNGC 5 cut(s) 243, 1069, 1186, 1224, 1283
HaeIII GGCC 2 cut(s) 76, 258
HapII CCGG 1 cut(s) 461
HgaI GACGC 1 cut(s) 107
HhaI GCGC 2 cut(s) 605, 682
Hin1II CATG 6 cut(s) 483, 630, 658, 973, 1166, 1340
Hin6I GCGC 2 cut(s) 603, 680
HinP1I GCGC 2 cut(s) 603, 680
HincII GTYRAC 1 cut(s) 750
HindII GTYRAC 1 cut(s) 750
HinfI GANTC 8 cut(s) 68, 301, 751, 974, 1015, 1210, 1241, 1358
HpaII CCGG 1 cut(s) 461
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 750
Hpy188III TCNNGA 9 cut(s) 162, 305, 610, 662, 813, 1086, 1149, 1207, 1214
Hpy8I GTNNAC 1 cut(s) 750
HpyAV CCTTC 4 cut(s) 80, 561, 770, 1134
HpyCH4III ACNGT 6 cut(s) 525, 853, 858, 1001, 1046, 1376
HpyCH4IV ACGT 1 cut(s) 761
HpyF10VI GCNNNNNNNGC 6 cut(s) 282, 286, 429, 548, 939, 1163
HpyF3I CTNAG 3 cut(s) 683, 1099, 1208
HpySE526I ACGT 1 cut(s) 761
Hsp92II CATG 6 cut(s) 483, 630, 658, 973, 1166, 1340
HspAI GCGC 2 cut(s) 603, 680
Kzo9I GATC 5 cut(s) 155, 686, 691, 902, 1159
LguI GCTCTTC 1 cut(s) 1088
LmnI GCTCC 2 cut(s) 106, 112
Lsp1109I GCAGC 4 cut(s) 229, 1080, 1172, 1235
LweI GCATC 6 cut(s) 155, 289, 466, 667, 667, 1294
MabI ACCWGGT 1 cut(s) 409
MaeI CTAG 4 cut(s) 359, 378, 1065, 1086
MaeII ACGT 1 cut(s) 761
MaeIII GTNAC 2 cut(s) 95, 530
MalI GATC 5 cut(s) 157, 688, 693, 904, 1161
MboI GATC 5 cut(s) 155, 686, 691, 902, 1159
MboII GAAGA 8 cut(s) 350, 393, 503, 801, 828, 1105, 1242, 1266
MfeI CAATTG 1 cut(s) 91
MflI RGATCY 2 cut(s) 691, 902
MlsI TGGCCA 1 cut(s) 76
MluCI AATT 9 cut(s) 56, 91, 226, 501, 582, 589, 622, 915, 1340
MluNI TGGCCA 1 cut(s) 76
MlyI GAGTC 5 cut(s) 77, 745, 1219, 1235, 1352
MmeI TCCRAC 3 cut(s) 110, 170, 1269
Mox20I TGGCCA 1 cut(s) 76
Mph1103I ATGCAT 2 cut(s) 481, 660
MroXI GAANNNNTTC 1 cut(s) 501
MscI TGGCCA 1 cut(s) 76
MseI TTAA 4 cut(s) 455, 585, 705, 833
MslI CAYNNNNRTG 2 cut(s) 163, 799
Msp20I TGGCCA 1 cut(s) 76
MspA1I CMGCKG 1 cut(s) 1185
MspI CCGG 1 cut(s) 461
MspR9I CCNGG 6 cut(s) 151, 255, 411, 461, 564, 1331
MunI CAATTG 1 cut(s) 91
Mva1269I GAATGC 2 cut(s) 43, 605
MvaI CCWGG 5 cut(s) 151, 255, 411, 564, 1331
MwoI GCNNNNNNNGC 6 cut(s) 282, 286, 429, 548, 939, 1163
NciI CCSGG 1 cut(s) 461
NdeI CATATG 2 cut(s) 766, 1396
NdeII GATC 5 cut(s) 155, 686, 691, 902, 1159
NheI GCTAGC 1 cut(s) 1064
NlaIII CATG 6 cut(s) 483, 630, 658, 973, 1166, 1340
NlaIV GGNNCC 3 cut(s) 114, 1180, 1328
NmuCI GTSAC 1 cut(s) 95
NsiI ATGCAT 2 cut(s) 481, 660
NspI RCATGY 1 cut(s) 658
PciSI GCTCTTC 1 cut(s) 1088
PctI GAATGC 2 cut(s) 43, 605
PdmI GAANNNNTTC 1 cut(s) 501
PfeI GAWTC 3 cut(s) 301, 974, 1015
PflFI GACNNNGTC 1 cut(s) 1259
PkrI GCNGC 5 cut(s) 244, 1070, 1187, 1225, 1284
PleI GAGTC 5 cut(s) 76, 745, 1218, 1235, 1352
PpsI GAGTC 5 cut(s) 76, 745, 1218, 1235, 1352
PsiI TTATAA 1 cut(s) 1311
Psp6I CCWGG 5 cut(s) 149, 253, 409, 562, 1329
PspGI CCWGG 5 cut(s) 149, 253, 409, 562, 1329
PspN4I GGNNCC 3 cut(s) 114, 1180, 1328
PspPI GGNCC 1 cut(s) 143
PsuI RGATCY 2 cut(s) 691, 902
PsyI GACNNNGTC 1 cut(s) 1259
PvuII CAGCTG 1 cut(s) 1185
RsaI GTAC 3 cut(s) 446, 855, 1003
RsaNI GTAC 3 cut(s) 445, 854, 1002
RseI CAYNNNNRTG 2 cut(s) 163, 799
SapI GCTCTTC 1 cut(s) 1088
SaqAI TTAA 4 cut(s) 455, 585, 705, 833
SatI GCNGC 5 cut(s) 243, 1069, 1186, 1224, 1283
Sau3AI GATC 5 cut(s) 155, 686, 691, 902, 1159
Sau96I GGNCC 1 cut(s) 143
SchI GAGTC 5 cut(s) 77, 745, 1219, 1235, 1352
ScrFI CCNGG 6 cut(s) 151, 255, 411, 461, 564, 1331
SexAI ACCWGGT 1 cut(s) 409
SfaNI GCATC 6 cut(s) 155, 289, 466, 667, 667, 1294
SfcI CTRYAG 1 cut(s) 741
SinI GGWCC 1 cut(s) 143
SmiMI CAYNNNNRTG 2 cut(s) 163, 799
Sse9I AATT 9 cut(s) 56, 91, 226, 501, 582, 589, 622, 915, 1340
SsiI CCGC 5 cut(s) 245, 804, 940, 1155, 1282
SspMI CTAG 4 cut(s) 359, 378, 1065, 1086
StyD4I CCNGG 6 cut(s) 149, 253, 409, 459, 562, 1329
TaaI ACNGT 6 cut(s) 525, 853, 858, 1001, 1046, 1376
TaiI ACGT 1 cut(s) 764
TaqI TCGA 2 cut(s) 66, 415
TasI AATT 9 cut(s) 56, 91, 226, 501, 582, 589, 622, 915, 1340
TatI WGTACW 1 cut(s) 1001
TauI GCSGC 1 cut(s) 1285
TfiI GAWTC 3 cut(s) 301, 974, 1015
Tru1I TTAA 4 cut(s) 455, 585, 705, 833
Tru9I TTAA 4 cut(s) 455, 585, 705, 833
TscAI CASTG 2 cut(s) 278, 1051
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 4 cut(s) 242, 1068, 1185, 1223
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 6 cut(s) 615, 958, 966, 1233, 1353, 1365
TspRI CASTG 2 cut(s) 278, 1051
Tth111I GACNNNGTC 1 cut(s) 1259
VpaK11BI GGWCC 1 cut(s) 143
XapI RAATTY 4 cut(s) 56, 501, 622, 1340
XbaI TCTAGA 1 cut(s) 1085
XceI RCATGY 1 cut(s) 658
XcmI CCANNNNNNNNNTGG 1 cut(s) 524
XmnI GAANNNNTTC 1 cut(s) 501
XspI CTAG 4 cut(s) 359, 378, 1065, 1086
Zsp2I ATGCAT 2 cut(s) 481, 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.