MD14G1071300.v1.1

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011779
Physical Location & Seq
Forward (+)
7869358 .. 7871425
2068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1071300.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGTCGCCTTTGTCTCTCTTGATTTGCTTTCTGACCTATTTTTCTGTCTGCAATGGTTACGCTTCTGCTGTACCTCTTGATGGAGAACTCCCAAATGGACACTTCGAAGAATCACCACATCCTTCAAATTTGAAGAAAACGGTGATCATCGGCAAGTATTCACTCCCAGAGTGGGAAATCAGCGGCTTGGTGGAGTACGTCAAAGGTGGAGCCCAACCAGGCGGTTTCTTCTTCAACGTGCCACGTGGGGTCCACGCAGTGAGACTCGGCAACGAGGCCTCCATTTCTCAGAGCGTGAATGTTAGCCCGAGTTCAGTCTACTCGCTCACTTTTGGGGCTACAAGAACTTGTGCCCAGGATGAGGTTTTAACAGTCTCAGTACCTGGTCAGTCTACTGACCTCTCCCTTCAAACTGTCTTCAGCAGCAATGGTGGTGATACTTATGCTTGGGCTTTCAAGGCTATGTCCAACGTTGTTAAGATTACATTACACAACCCTGGGGTTCAGGAAGACCCCGCTTGTGGGCCTCTACTGGATGCTATTGCAATCAAGCAGATGCTTCCTTTCAACTACACAAGGGGTAACCTAGTGAAAAATGGAGGGTTTGAAAAAGGCCCTCATGTGTTTAAGAACTTCTCAACCGGAATCCTCATCCCTCCCAAGGTGAATGACCCGTACTCACCACTGCCTGGATGGATCATTGAATCCCTGAAGCCTGTGAAATACATAGACAGGAAGCATTTTGCGGTTCCTCGCGGACTTGCAGCCATTGAGTTGGTTGCAGGGAGAGAAAGTGCAATTGCTCAAATTATTAGAACCGTCCCAAACAAATCCTACAACCTTATCTTCACCATCGGAGACGCAAAGAACGGTTGCCACGGATCGATGATGGTCGAAGCCTTTGCTGGCAAGGAAACCCTAAAAGTTCCTTTTGTGTCTGCAGGAAAAGGTGGCTTCAAAACTGCAATTCTCAAGTTCCAAGCAACTTCATTCAGAACAAGATTAACATTTTACAGTGCCTACTATCACACAAAGCTTGATGACTATGGTCATATGTGTGGCCCCGTCTTGGATAATGTCATTGTTTTTCCAGTCGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

367

Amino Acids

39.61

Weight (kDa)

8.74

Isoelectric Point (pI)

36.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF642 PF04862 29 - 184 6.5e-63 Protein of unknown function (DUF642)
DUF642 PF04862 196 - 362 3.7e-14 Protein of unknown function (DUF642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011779)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41800 AT2G41810
fragaria_vesca FvH4_6g23670
malus_domestica MD14G1071300.v1.1
prunus_persica Prupe.7G061700_v2.0.a1
pyrus_communis pycom14g06080
rosa_chinensis RchiOBHm_Chr3g0482101
rosa_laevigata RLG00000023374
rosa_multiflora Rmu_co8504877.1_g000001 Rmu_sc0000192.1_g000028 Rmu_sc0001738.1_g000034
rosa_roxburghii Rroxscaffold_6G00399700 Rroxscaffold_6G00400070
rosa_rugosa Rorug03G0194800
rosa_samantha Rh3AG246000 Rh3BG281100 Rh3CG277200 Rh3DG273500
rosa_wichuraiana Rw3G022000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 689
AccI GTMKAC 2 cut(s) 318, 392
AccII CGCG 1 cut(s) 756
AciI CCGC 5 cut(s) 183, 222, 516, 746, 756
AclI AACGTT 1 cut(s) 471
AclWI GGATC 2 cut(s) 704, 889
AcsI RAATTY 1 cut(s) 127
AcuI CTGAAG 2 cut(s) 403, 731
AcvI CACGTG 1 cut(s) 245
AdeI CACNNNGTG 1 cut(s) 259
AfaI GTAC 4 cut(s) 72, 197, 381, 677
AfiI CCNNNNNNNGG 8 cut(s) 80, 172, 361, 521, 522, 661, 689, 1069
AgsI TTSAA 9 cut(s) 126, 133, 235, 410, 457, 568, 608, 704, 958
AjnI CCWGG 5 cut(s) 217, 354, 382, 496, 688
AluBI AGCT 1 cut(s) 1036
AluI AGCT 1 cut(s) 1036
Alw26I GTCTC 4 cut(s) 18, 256, 379, 852
AlwI GGATC 2 cut(s) 704, 889
AlwNI CAGNNNCTG 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 307
AoxI GGCC 4 cut(s) 276, 524, 613, 1060
ApeKI GCWGC 2 cut(s) 423, 764
ApoI RAATTY 1 cut(s) 127
AspS9I GGNCC 4 cut(s) 250, 524, 614, 1061
AsuHPI GGTGA 6 cut(s) 105, 154, 446, 672, 676, 841
AsuII TTCGAA 1 cut(s) 105
AvaI CYCGRG 1 cut(s) 307
AvaII GGWCC 1 cut(s) 250
BaeGI GKGCMC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 214
BbrPI CACGTG 1 cut(s) 245
BbsI GAAGAC 2 cut(s) 409, 516
BbvI GCAGC 2 cut(s) 435, 776
BccI CCATC 4 cut(s) 74, 687, 860, 883
BcgI CGANNNNNNTGC 2 cut(s) 884, 918
BciT130I CCWGG 5 cut(s) 219, 356, 384, 498, 690
BclI TGATCA 1 cut(s) 144
BcoDI GTCTC 4 cut(s) 18, 256, 379, 852
BfaI CTAG 1 cut(s) 587
BfmI CTRYAG 1 cut(s) 939
BisI GCNGC 3 cut(s) 184, 424, 765
BlsI GCNGC 3 cut(s) 185, 425, 766
Bme1390I CCNGG 5 cut(s) 219, 356, 384, 498, 690
Bme18I GGWCC 1 cut(s) 250
BmeT110I CYCGRG 1 cut(s) 307
BmgT120I GGNCC 4 cut(s) 250, 524, 614, 1061
BmiI GGNNCC 4 cut(s) 211, 251, 750, 1063
BmrFI CCNGG 5 cut(s) 219, 356, 384, 498, 690
BmsI GCATC 2 cut(s) 526, 546
BoxI GACNNNNGTC 1 cut(s) 1047
BpiI GAAGAC 2 cut(s) 409, 516
Bpu14I TTCGAA 1 cut(s) 105
BpuEI CTTGAG 1 cut(s) 956
Bsa29I ATCGAT 1 cut(s) 884
BsaAI YACGTR 1 cut(s) 245
BsaJI CCNNGG 5 cut(s) 354, 496, 497, 660, 877
BsaWI WCCGGW 1 cut(s) 641
BsaXI ACNNNNNCTCC 4 cut(s) 263, 293, 778, 808
Bsc4I CCNNNNNNNGG 8 cut(s) 80, 172, 361, 521, 522, 661, 689, 1069
Bse1I ACTGG 2 cut(s) 537, 1091
Bse3DI GCAATG 2 cut(s) 58, 433
BseBI CCWGG 5 cut(s) 219, 356, 384, 498, 690
BseCI ATCGAT 1 cut(s) 884
BseDI CCNNGG 5 cut(s) 354, 496, 497, 660, 877
BseGI GGATG 5 cut(s) 118, 364, 541, 651, 698
BseLI CCNNNNNNNGG 8 cut(s) 80, 172, 361, 521, 522, 661, 689, 1069
BseMI GCAATG 2 cut(s) 58, 433
BseMII CTCAG 2 cut(s) 302, 390
BseNI ACTGG 2 cut(s) 537, 1091
BseSI GKGCMC 1 cut(s) 355
BseXI GCAGC 2 cut(s) 435, 776
Bsh1236I CGCG 1 cut(s) 756
BshFI GGCC 4 cut(s) 278, 526, 615, 1062
BshVI ATCGAT 1 cut(s) 884
BsiHKCI CYCGRG 1 cut(s) 307
BsiSI CCGG 1 cut(s) 642
BslFI GGGAC 1 cut(s) 806
BslI CCNNNNNNNGG 8 cut(s) 80, 172, 361, 521, 522, 661, 689, 1069
BsmAI GTCTC 4 cut(s) 18, 256, 379, 852
BsmBI CGTCTC 1 cut(s) 852
BsmFI GGGAC 1 cut(s) 806
BsnI GGCC 4 cut(s) 278, 526, 615, 1062
BsoBI CYCGRG 1 cut(s) 307
Bsp119I TTCGAA 1 cut(s) 105
Bsp1286I GDGCHC 2 cut(s) 214, 355
Bsp143I GATC 3 cut(s) 144, 696, 881
BspACI CCGC 5 cut(s) 183, 222, 516, 746, 756
BspANI GGCC 4 cut(s) 278, 526, 615, 1062
BspCNI CTCAG 2 cut(s) 301, 389
BspDI ATCGAT 1 cut(s) 884
BspFNI CGCG 1 cut(s) 756
BspLI GGNNCC 4 cut(s) 211, 251, 750, 1063
BspMAI CTGCAG 1 cut(s) 943
BspPI GGATC 2 cut(s) 704, 889
BspT104I TTCGAA 1 cut(s) 105
BsrDI GCAATG 2 cut(s) 58, 433
BsrI ACTGG 2 cut(s) 537, 1091
BssECI CCNNGG 5 cut(s) 354, 496, 497, 660, 877
BssMI GATC 3 cut(s) 144, 696, 881
BssT1I CCWWGG 1 cut(s) 660
Bst2UI CCWGG 5 cut(s) 219, 356, 384, 498, 690
Bst4CI ACNGT 6 cut(s) 142, 373, 415, 820, 872, 1016
BstBAI YACGTR 1 cut(s) 245
BstBI TTCGAA 1 cut(s) 105
BstC8I GCNNGC 1 cut(s) 907
BstDEI CTNAG 2 cut(s) 288, 376
BstDSI CCRYGG 1 cut(s) 877
BstEII GGTNACC 1 cut(s) 581
BstF5I GGATG 5 cut(s) 118, 364, 541, 651, 698
BstFNI CGCG 1 cut(s) 756
BstKTI GATC 3 cut(s) 147, 699, 884
BstMAI GTCTC 4 cut(s) 18, 256, 379, 852
BstMBI GATC 3 cut(s) 144, 696, 881
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 5 cut(s) 219, 356, 384, 498, 690
BstPAI GACNNNNGTC 1 cut(s) 1047
BstPI GGTNACC 1 cut(s) 581
BstSCI CCNGG 5 cut(s) 217, 354, 382, 496, 688
BstSFI CTRYAG 1 cut(s) 939
BstSLI GKGCMC 1 cut(s) 355
BstUI CGCG 1 cut(s) 756
BstV1I GCAGC 2 cut(s) 435, 776
BstV2I GAAGAC 2 cut(s) 409, 516
BstXI CCANNNNNNTGG 1 cut(s) 775
Bsu15I ATCGAT 1 cut(s) 884
BsuRI GGCC 4 cut(s) 278, 526, 615, 1062
BsuTUI ATCGAT 1 cut(s) 884
BtgI CCRYGG 1 cut(s) 877
BtsCI GGATG 5 cut(s) 118, 364, 541, 651, 698
BtsI GCAGTG 2 cut(s) 264, 683
BtsIMutI CAGTG 3 cut(s) 264, 683, 1021
Cac8I GCNNGC 1 cut(s) 907
CaiI CAGNNNCTG 1 cut(s) 383
Cfr13I GGNCC 4 cut(s) 250, 524, 614, 1061
ClaI ATCGAT 1 cut(s) 884
CseI GACGC 1 cut(s) 869
CsiI ACCWGGT 1 cut(s) 382
Csp6I GTAC 4 cut(s) 71, 196, 380, 676
CviAII CATG 1 cut(s) 620
CviQI GTAC 4 cut(s) 71, 196, 380, 676
DdeI CTNAG 2 cut(s) 288, 376
DpnI GATC 3 cut(s) 146, 698, 883
DpnII GATC 3 cut(s) 144, 696, 881
DraIII CACNNNGTG 1 cut(s) 259
Eco130I CCWWGG 1 cut(s) 660
Eco147I AGGCCT 1 cut(s) 278
Eco24I GRGCYC 1 cut(s) 214
Eco47I GGWCC 1 cut(s) 250
Eco57I CTGAAG 2 cut(s) 403, 731
Eco72I CACGTG 1 cut(s) 245
Eco88I CYCGRG 1 cut(s) 307
Eco91I GGTNACC 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 614
EcoO65I GGTNACC 1 cut(s) 581
EcoRII CCWGG 5 cut(s) 217, 354, 382, 496, 688
EcoT14I CCWWGG 1 cut(s) 660
EcoT38I GRGCYC 1 cut(s) 214
ErhI CCWWGG 1 cut(s) 660
Esp3I CGTCTC 1 cut(s) 852
FaeI CATG 1 cut(s) 623
FaiI YATR 7 cut(s) 444, 464, 621, 728, 1047, 1053, 1055
FaqI GGGAC 1 cut(s) 806
FatI CATG 1 cut(s) 619
FauI CCCGC 1 cut(s) 523
FauNDI CATATG 1 cut(s) 1053
FbaI TGATCA 1 cut(s) 144
FblI GTMKAC 2 cut(s) 318, 392
Fnu4HI GCNGC 3 cut(s) 184, 424, 765
FokI GGATG 5 cut(s) 105, 371, 548, 638, 705
FriOI GRGCYC 1 cut(s) 214
Fsp4HI GCNGC 3 cut(s) 184, 424, 765
FspBI CTAG 1 cut(s) 587
GluI GCNGC 3 cut(s) 184, 424, 765
HaeIII GGCC 4 cut(s) 278, 526, 615, 1062
HapII CCGG 1 cut(s) 642
HgaI GACGC 1 cut(s) 869
Hin1II CATG 1 cut(s) 623
HindIII AAGCTT 1 cut(s) 1034
HinfI GANTC 4 cut(s) 110, 264, 645, 704
HpaII CCGG 1 cut(s) 642
HphI GGTGA 6 cut(s) 105, 154, 446, 672, 676, 841
Hpy166II GTNNAC 3 cut(s) 253, 319, 393
Hpy188I TCNGA 4 cut(s) 33, 291, 857, 995
Hpy188III TCNNGA 3 cut(s) 19, 77, 506
Hpy8I GTNNAC 3 cut(s) 253, 319, 393
HpyAV CCTTC 2 cut(s) 132, 416
HpyCH4III ACNGT 6 cut(s) 142, 373, 415, 820, 872, 1016
HpyCH4IV ACGT 4 cut(s) 198, 237, 244, 471
HpyCH4V TGCA 7 cut(s) 51, 545, 764, 782, 797, 941, 965
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 2 cut(s) 288, 376
HpySE526I ACGT 4 cut(s) 198, 237, 244, 471
Hsp92II CATG 1 cut(s) 623
Ksp22I TGATCA 1 cut(s) 144
Kzo9I GATC 3 cut(s) 144, 696, 881
LmnI GCTCC 1 cut(s) 209
Lsp1109I GCAGC 2 cut(s) 435, 776
LweI GCATC 2 cut(s) 526, 546
MabI ACCWGGT 1 cut(s) 382
MaeI CTAG 1 cut(s) 587
MaeII ACGT 4 cut(s) 198, 237, 244, 471
MaeIII GTNAC 2 cut(s) 56, 581
MalI GATC 3 cut(s) 146, 698, 883
MboI GATC 3 cut(s) 144, 696, 881
MboII GAAGA 7 cut(s) 119, 145, 220, 223, 409, 521, 838
MfeI CAATTG 1 cut(s) 798
MhlI GDGCHC 2 cut(s) 214, 355
MluCI AATT 4 cut(s) 127, 798, 807, 966
MlyI GAGTC 1 cut(s) 258
MmeI TCCRAC 1 cut(s) 492
MseI TTAA 4 cut(s) 368, 477, 627, 1004
MslI CAYNNNNRTG 1 cut(s) 1056
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 1 cut(s) 642
MspR9I CCNGG 5 cut(s) 219, 356, 384, 498, 690
MunI CAATTG 1 cut(s) 798
MvaI CCWGG 5 cut(s) 219, 356, 384, 498, 690
MvnI CGCG 1 cut(s) 756
MwoI GCNNNNNNNGC 1 cut(s) 458
NdeI CATATG 1 cut(s) 1053
NdeII GATC 3 cut(s) 144, 696, 881
NlaIII CATG 1 cut(s) 623
NlaIV GGNNCC 4 cut(s) 211, 251, 750, 1063
NmeAIII GCCGAG 1 cut(s) 246
NspV TTCGAA 1 cut(s) 105
PasI CCCWGGG 1 cut(s) 497
PceI AGGCCT 1 cut(s) 278
PfeI GAWTC 3 cut(s) 110, 645, 704
PflMI CCANNNNNTGG 1 cut(s) 689
PkrI GCNGC 3 cut(s) 185, 425, 766
PleI GAGTC 1 cut(s) 258
PmaCI CACGTG 1 cut(s) 245
PmlI CACGTG 1 cut(s) 245
PpsI GAGTC 1 cut(s) 258
Ppu21I YACGTR 1 cut(s) 245
PshAI GACNNNNGTC 1 cut(s) 1047
Psp1406I AACGTT 1 cut(s) 471
Psp6I CCWGG 5 cut(s) 217, 354, 382, 496, 688
PspCI CACGTG 1 cut(s) 245
PspEI GGTNACC 1 cut(s) 581
PspGI CCWGG 5 cut(s) 217, 354, 382, 496, 688
PspN4I GGNNCC 4 cut(s) 211, 251, 750, 1063
PspPI GGNCC 4 cut(s) 250, 524, 614, 1061
PstI CTGCAG 1 cut(s) 943
PstNI CAGNNNCTG 1 cut(s) 383
RsaI GTAC 4 cut(s) 72, 197, 381, 677
RsaNI GTAC 4 cut(s) 71, 196, 380, 676
RseI CAYNNNNRTG 1 cut(s) 1056
SaqAI TTAA 4 cut(s) 368, 477, 627, 1004
SatI GCNGC 3 cut(s) 184, 424, 765
Sau3AI GATC 3 cut(s) 144, 696, 881
Sau96I GGNCC 4 cut(s) 250, 524, 614, 1061
SchI GAGTC 1 cut(s) 258
ScrFI CCNGG 5 cut(s) 219, 356, 384, 498, 690
SduI GDGCHC 2 cut(s) 214, 355
SexAI ACCWGGT 1 cut(s) 382
SfaNI GCATC 2 cut(s) 526, 546
SfcI CTRYAG 1 cut(s) 939
SfuI TTCGAA 1 cut(s) 105
SinI GGWCC 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 1056
SmlI CTYRAG 1 cut(s) 971
SmoI CTYRAG 1 cut(s) 971
Sse9I AATT 4 cut(s) 127, 798, 807, 966
SseBI AGGCCT 1 cut(s) 278
SsiI CCGC 5 cut(s) 183, 222, 516, 746, 756
SspMI CTAG 1 cut(s) 587
StuI AGGCCT 1 cut(s) 278
StyD4I CCNGG 5 cut(s) 217, 354, 382, 496, 688
StyI CCWWGG 1 cut(s) 660
TaaI ACNGT 6 cut(s) 142, 373, 415, 820, 872, 1016
TaiI ACGT 4 cut(s) 201, 240, 247, 474
TaqI TCGA 3 cut(s) 105, 884, 894
TasI AATT 4 cut(s) 127, 798, 807, 966
TauI GCSGC 1 cut(s) 186
TfiI GAWTC 3 cut(s) 110, 645, 704
Tru1I TTAA 4 cut(s) 368, 477, 627, 1004
Tru9I TTAA 4 cut(s) 368, 477, 627, 1004
TscAI CASTG 3 cut(s) 264, 690, 1021
TseI GCWGC 2 cut(s) 423, 764
TspDTI ATGAA 1 cut(s) 978
TspGWI ACGGA 1 cut(s) 894
TspRI CASTG 3 cut(s) 264, 690, 1021
Van91I CCANNNNNTGG 1 cut(s) 689
VpaK11BI GGWCC 1 cut(s) 250
XapI RAATTY 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 690
XmiI GTMKAC 2 cut(s) 318, 392
XspI CTAG 1 cut(s) 587
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.