Rw3G022000

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0011779
Physical Location & Seq
Forward (+)
26775968 .. 26778742
2775 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G022000.1

Sequence Viewer

Length: 1116 bp
ATGGCTCCTCTCTCCTTCTTTCTTTGCTTTCTGGCCACTATCGTTCTCTGCACTGCTTATGCTTCTGCAGCTGCTACTCTACACCTTGATGGGGAACTACCAAATGGACACTTCGAAGAATCACCCAGTTCCTCAAACCTAAAGAAAACAGTCATCGTCGGGAAACACTCACTCCCAAAGTGGGAAATCGACGGCCTGGTCGAGTACATCGCAGGTGGGCCTCAGCCAGGTGGCTTCTACTTTCCGGTGGCGCGTGGCGTCCACGCCGTGAGGCTCGGCAACGAGGCCTCCATTTCTCAGAACGTCAATGTCAACCCGGGTTCGGTCTACTCTCTCACTTTTGGCACCACAAGAACTTGTGCTCAAGACGAGGTGCTCAAGGTCTCCGTGCCGGGTCAGTCTGCAGAGCTTTCCATACAGACTCTGTTCAGCAGCGATGGGGGTGACACTTATGCATGGGCCTTTAAGGCGACTTCCAAGGTTGTTAGGGTTACGTTCCACAACCCTGGGATTCAAGAGGACCCCGCTTGCGGTCCTCTATTGGATGCTATTGCAATCAAGGAGATCATACTTCCTCAGAAGTATATAAGAGGTAATTTAGTCAAAAATGGTGGCTTTGAAAACGGTCCTCATGTATTCAAGAATTTCTCAACCGGAGTCCTCCTCCCTCCAAAGTTACTGGACCAAATCTCACCGCTGCCTGGATGGATCATCGAATCCCTAAAGCCTGTGAAGTACATTGACAGGAAGCACTTCTCAGTTCCAGTCGGATTCGCAGCAATTGAATTGGTTGCAGGAAGGGAGAGTGCCATTGCTCAAATTATTAGAACAATCCCAAACAAATCCTACAACCTTACCTTCACCATTGGGGATGCAAAGAATGGTTGTCATGGATCAATGATGGTTGAAGCCTTCGCTGGTAAGGAAACCCTAAAAGTTCCTTATGTTTCTCAAGGCAAGGGTGGATATAGAACTGCAAGCTTCAAGTTCCAAGCAGTTTCCCCAAGAACTAGAATAACATTTTACAGTGCATACTATCACACAAAGCTTCATGATTTTGGTCATATGTGTGGCCCTGTCTTAGACAATGTCATTGTCTTTCCAACTAGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

371

Amino Acids

40.26

Weight (kDa)

8.87

Isoelectric Point (pI)

33.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF642 PF04862 32 - 187 2e-63 Protein of unknown function (DUF642)
DUF642 PF04862 200 - 366 4.2e-15 Protein of unknown function (DUF642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011779)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41800 AT2G41810
fragaria_vesca FvH4_6g23670
malus_domestica MD14G1071300.v1.1
prunus_persica Prupe.7G061700_v2.0.a1
pyrus_communis pycom14g06080
rosa_chinensis RchiOBHm_Chr3g0482101
rosa_laevigata RLG00000023374
rosa_multiflora Rmu_co8504877.1_g000001 Rmu_sc0000192.1_g000028 Rmu_sc0001738.1_g000034
rosa_roxburghii Rroxscaffold_6G00399700 Rroxscaffold_6G00400070
rosa_rugosa Rorug03G0194800
rosa_samantha Rh3AG246000 Rh3BG281100 Rh3CG277200 Rh3DG273500
rosa_wichuraiana Rw3G022000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 203
AasI GACNNNNNNGTC 1 cut(s) 197
Acc36I ACCTGC 1 cut(s) 203
AccB1I GGYRCC 1 cut(s) 344
AccI GTMKAC 1 cut(s) 327
AccII CGCG 1 cut(s) 253
AciI CCGC 3 cut(s) 525, 531, 695
AclWI GGATC 2 cut(s) 716, 901
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 1 cut(s) 643
AcyI GRCGYC 1 cut(s) 258
AdeI CACNNNGTG 1 cut(s) 268
AfaI GTAC 2 cut(s) 206, 737
AfiI CCNNNNNNNGG 6 cut(s) 91, 181, 227, 322, 530, 701
AgsI TTSAA 6 cut(s) 515, 620, 640, 785, 908, 985
AjnI CCWGG 4 cut(s) 195, 226, 505, 700
AluBI AGCT 4 cut(s) 71, 409, 981, 1048
AluI AGCT 4 cut(s) 71, 409, 981, 1048
Alw21I GWGCWC 2 cut(s) 364, 378
Alw26I GTCTC 1 cut(s) 388
AlwI GGATC 2 cut(s) 716, 901
AlwNI CAGNNNCTG 1 cut(s) 424
Ama87I CYCGRG 1 cut(s) 316
AoxI GGCC 6 cut(s) 33, 193, 218, 285, 459, 1072
ApeKI GCWGC 5 cut(s) 68, 71, 432, 697, 776
ApoI RAATTY 1 cut(s) 643
Asp700I GAANNNNTTC 1 cut(s) 752
AspLEI GCGC 1 cut(s) 253
AspS9I GGNCC 7 cut(s) 218, 459, 520, 533, 626, 682, 1073
AsuC2I CCSGG 3 cut(s) 317, 318, 393
AsuHPI GGTGA 4 cut(s) 114, 455, 684, 853
AsuII TTCGAA 1 cut(s) 114
AvaI CYCGRG 1 cut(s) 316
AvaII GGWCC 4 cut(s) 520, 533, 626, 682
BalI TGGCCA 1 cut(s) 35
BanI GGYRCC 1 cut(s) 344
Bbv12I GWGCWC 2 cut(s) 364, 378
BbvCI CCTCAGC 1 cut(s) 222
BbvI GCAGC 5 cut(s) 58, 80, 444, 684, 788
BccI CCATC 4 cut(s) 83, 431, 699, 895
BceAI ACGGC 2 cut(s) 208, 251
BciT130I CCWGG 4 cut(s) 197, 228, 507, 702
BcnI CCSGG 3 cut(s) 317, 318, 393
BcoDI GTCTC 1 cut(s) 388
BfaI CTAG 2 cut(s) 1011, 1107
BfmI CTRYAG 2 cut(s) 66, 402
BfuAI ACCTGC 1 cut(s) 203
BglI GCCNNNNNGGC 1 cut(s) 467
BisI GCNGC 5 cut(s) 69, 72, 433, 698, 777
BlsI GCNGC 5 cut(s) 70, 73, 434, 699, 778
Bme1390I CCNGG 7 cut(s) 197, 228, 317, 318, 393, 507, 702
Bme18I GGWCC 4 cut(s) 520, 533, 626, 682
BmeT110I CYCGRG 1 cut(s) 316
BmgT120I GGNCC 7 cut(s) 218, 459, 520, 533, 626, 682, 1073
BmiI GGNNCC 3 cut(s) 6, 346, 522
BmrFI CCNGG 7 cut(s) 197, 228, 317, 318, 393, 507, 702
BmrI ACTGGG 1 cut(s) 120
BmsI GCATC 2 cut(s) 535, 862
BmuI ACTGGG 1 cut(s) 120
BplI GAGNNNNNCTC 2 cut(s) 648, 680
Bpu10I CCTNAGC 1 cut(s) 222
Bpu14I TTCGAA 1 cut(s) 114
BpuEI CTTGAG 3 cut(s) 348, 362, 936
BpuMI CCSGG 3 cut(s) 317, 318, 393
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 388
BsaJI CCNNGG 4 cut(s) 316, 477, 505, 506
BsaWI WCCGGW 2 cut(s) 244, 653
BsaXI ACNNNNNCTCC 4 cut(s) 156, 186, 272, 302
Bsc4I CCNNNNNNNGG 6 cut(s) 91, 181, 227, 322, 530, 701
Bse1I ACTGG 3 cut(s) 126, 684, 764
Bse3DI GCAATG 1 cut(s) 810
BseBI CCWGG 4 cut(s) 197, 228, 507, 702
BseDI CCNNGG 4 cut(s) 316, 477, 505, 506
BseGI GGATG 3 cut(s) 550, 710, 877
BseLI CCNNNNNNNGG 6 cut(s) 91, 181, 227, 322, 530, 701
BseMI GCAATG 1 cut(s) 810
BseMII CTCAG 4 cut(s) 236, 311, 590, 771
BseNI ACTGG 3 cut(s) 126, 684, 764
BseRI GAGGAG 1 cut(s) 653
BseXI GCAGC 5 cut(s) 58, 80, 444, 684, 788
BsgI GTGCAG 1 cut(s) 34
Bsh1236I CGCG 1 cut(s) 253
BshFI GGCC 6 cut(s) 35, 195, 220, 287, 461, 1074
BshNI GGYRCC 1 cut(s) 344
BsiHKAI GWGCWC 2 cut(s) 364, 378
BsiHKCI CYCGRG 1 cut(s) 316
BsiSI CCGG 4 cut(s) 245, 317, 392, 654
BslI CCNNNNNNNGG 6 cut(s) 91, 181, 227, 322, 530, 701
BsmAI GTCTC 1 cut(s) 388
BsnI GGCC 6 cut(s) 35, 195, 220, 287, 461, 1074
Bso31I GGTCTC 1 cut(s) 388
BsoBI CYCGRG 1 cut(s) 316
Bsp119I TTCGAA 1 cut(s) 114
Bsp1286I GDGCHC 2 cut(s) 364, 378
Bsp143I GATC 3 cut(s) 564, 708, 893
BspACI CCGC 3 cut(s) 525, 531, 695
BspANI GGCC 6 cut(s) 35, 195, 220, 287, 461, 1074
BspCNI CTCAG 4 cut(s) 235, 310, 589, 770
BspFNI CGCG 1 cut(s) 253
BspHI TCATGA 1 cut(s) 1051
BspLI GGNNCC 3 cut(s) 6, 346, 522
BspMAI CTGCAG 2 cut(s) 70, 406
BspMI ACCTGC 1 cut(s) 203
BspPI GGATC 2 cut(s) 716, 901
BspT104I TTCGAA 1 cut(s) 114
BspT107I GGYRCC 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 388
BsrDI GCAATG 1 cut(s) 810
BsrI ACTGG 3 cut(s) 126, 684, 764
BssECI CCNNGG 4 cut(s) 316, 477, 505, 506
BssMI GATC 3 cut(s) 564, 708, 893
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 1 cut(s) 477
Bst2UI CCWGG 4 cut(s) 197, 228, 507, 702
Bst4CI ACNGT 3 cut(s) 151, 626, 1028
BstACI GRCGYC 1 cut(s) 258
BstBI TTCGAA 1 cut(s) 114
BstC8I GCNNGC 2 cut(s) 529, 979
BstDEI CTNAG 5 cut(s) 222, 297, 576, 757, 1081
BstENI CCTNNNNNAGG 1 cut(s) 225
BstF5I GGATG 3 cut(s) 550, 710, 877
BstFNI CGCG 1 cut(s) 253
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 3 cut(s) 567, 711, 896
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 3 cut(s) 564, 708, 893
BstMWI GCNNNNNNNGC 2 cut(s) 68, 467
BstNI CCWGG 4 cut(s) 197, 228, 507, 702
BstSCI CCNGG 7 cut(s) 195, 226, 315, 316, 391, 505, 700
BstSFI CTRYAG 2 cut(s) 66, 402
BstUI CGCG 1 cut(s) 253
BstV1I GCAGC 5 cut(s) 58, 80, 444, 684, 788
BstXI CCANNNNNNTGG 1 cut(s) 506
BsuRI GGCC 6 cut(s) 35, 195, 220, 287, 461, 1074
BtgZI GCGATG 2 cut(s) 193, 450
BtsCI GGATG 3 cut(s) 550, 710, 877
BtsI GCAGTG 1 cut(s) 51
BtsIMutI CAGTG 2 cut(s) 51, 1033
BveI ACCTGC 1 cut(s) 203
Cac8I GCNNGC 2 cut(s) 529, 979
CaiI CAGNNNCTG 1 cut(s) 424
CciI TCATGA 1 cut(s) 1051
CfoI GCGC 1 cut(s) 253
Cfr13I GGNCC 7 cut(s) 218, 459, 520, 533, 626, 682, 1073
Cfr9I CCCGGG 1 cut(s) 316
CseI GACGC 1 cut(s) 247
Csp6I GTAC 2 cut(s) 205, 736
CspCI CAANNNNNGTGG 2 cut(s) 592, 627
CviAII CATG 4 cut(s) 456, 632, 890, 1052
CviQI GTAC 2 cut(s) 205, 736
DdeI CTNAG 5 cut(s) 222, 297, 576, 757, 1081
DpnI GATC 3 cut(s) 566, 710, 895
DpnII GATC 3 cut(s) 564, 708, 893
DraIII CACNNNGTG 1 cut(s) 268
DrdI GACNNNNNNGTC 1 cut(s) 197
DseDI GACNNNNNNGTC 1 cut(s) 197
EaeI YGGCCR 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 477
Eco147I AGGCCT 1 cut(s) 287
Eco31I GGTCTC 1 cut(s) 388
Eco47I GGWCC 4 cut(s) 520, 533, 626, 682
Eco88I CYCGRG 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 225
EcoO109I RGGNCCY 1 cut(s) 520
EcoRII CCWGG 4 cut(s) 195, 226, 505, 700
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 457
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 4 cut(s) 459, 635, 893, 1055
FatI CATG 4 cut(s) 455, 631, 889, 1051
FauI CCCGC 1 cut(s) 532
FauNDI CATATG 1 cut(s) 1065
FblI GTMKAC 1 cut(s) 327
Fnu4HI GCNGC 5 cut(s) 69, 72, 433, 698, 777
FokI GGATG 3 cut(s) 557, 717, 884
Fsp4HI GCNGC 5 cut(s) 69, 72, 433, 698, 777
FspBI CTAG 2 cut(s) 1011, 1107
GlaI GCGC 1 cut(s) 252
GluI GCNGC 5 cut(s) 69, 72, 433, 698, 777
HaeIII GGCC 6 cut(s) 35, 195, 220, 287, 461, 1074
HapII CCGG 4 cut(s) 245, 317, 392, 654
HgaI GACGC 1 cut(s) 247
HhaI GCGC 1 cut(s) 253
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 4 cut(s) 459, 635, 893, 1055
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HincII GTYRAC 1 cut(s) 313
HindII GTYRAC 1 cut(s) 313
HindIII AAGCTT 2 cut(s) 979, 1046
HinfI GANTC 6 cut(s) 119, 421, 511, 657, 716, 771
HpaII CCGG 4 cut(s) 245, 317, 392, 654
HphI GGTGA 4 cut(s) 114, 455, 684, 853
Hpy166II GTNNAC 3 cut(s) 262, 313, 328
Hpy188I TCNGA 3 cut(s) 300, 579, 770
Hpy188III TCNNGA 5 cut(s) 160, 365, 515, 640, 1052
Hpy8I GTNNAC 3 cut(s) 262, 313, 328
Hpy99I CGWCG 2 cut(s) 161, 194
HpyAV CCTTC 4 cut(s) 25, 792, 868, 922
HpyCH4III ACNGT 3 cut(s) 151, 626, 1028
HpyCH4IV ACGT 2 cut(s) 303, 494
HpyCH4V TGCA 9 cut(s) 51, 68, 404, 455, 554, 794, 875, 977, 1031
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 467
HpyF3I CTNAG 5 cut(s) 222, 297, 576, 757, 1081
HpySE526I ACGT 2 cut(s) 303, 494
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 4 cut(s) 459, 635, 893, 1055
HspAI GCGC 1 cut(s) 251
Kzo9I GATC 3 cut(s) 564, 708, 893
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 5 cut(s) 58, 80, 444, 684, 788
LweI GCATC 2 cut(s) 535, 862
MaeI CTAG 2 cut(s) 1011, 1107
MaeII ACGT 2 cut(s) 303, 494
MaeIII GTNAC 3 cut(s) 443, 490, 675
MalI GATC 3 cut(s) 566, 710, 895
MboI GATC 3 cut(s) 564, 708, 893
MboII GAAGA 1 cut(s) 128
MfeI CAATTG 1 cut(s) 780
MhlI GDGCHC 2 cut(s) 364, 378
MlsI TGGCCA 1 cut(s) 35
MluCI AATT 5 cut(s) 595, 643, 780, 785, 819
MluNI TGGCCA 1 cut(s) 35
MlyI GAGTC 2 cut(s) 415, 666
MmeI TCCRAC 1 cut(s) 748
Mox20I TGGCCA 1 cut(s) 35
Mph1103I ATGCAT 1 cut(s) 457
MroXI GAANNNNTTC 1 cut(s) 752
MscI TGGCCA 1 cut(s) 35
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 2 cut(s) 87, 1068
Msp20I TGGCCA 1 cut(s) 35
MspA1I CMGCKG 2 cut(s) 71, 697
MspI CCGG 4 cut(s) 245, 317, 392, 654
MspR9I CCNGG 7 cut(s) 197, 228, 317, 318, 393, 507, 702
MunI CAATTG 1 cut(s) 780
MvaI CCWGG 4 cut(s) 197, 228, 507, 702
MvnI CGCG 1 cut(s) 253
MwoI GCNNNNNNNGC 2 cut(s) 68, 467
NciI CCSGG 3 cut(s) 317, 318, 393
NdeI CATATG 1 cut(s) 1065
NdeII GATC 3 cut(s) 564, 708, 893
NlaIII CATG 4 cut(s) 459, 635, 893, 1055
NlaIV GGNNCC 3 cut(s) 6, 346, 522
NmeAIII GCCGAG 1 cut(s) 255
NmuCI GTSAC 1 cut(s) 443
NsiI ATGCAT 1 cut(s) 457
NspV TTCGAA 1 cut(s) 114
PagI TCATGA 1 cut(s) 1051
PaqCI CACCTGC 1 cut(s) 203
PasI CCCWGGG 1 cut(s) 506
PceI AGGCCT 1 cut(s) 287
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 752
PfeI GAWTC 4 cut(s) 119, 511, 716, 771
PflFI GACNNNGTC 1 cut(s) 1088
PkrI GCNGC 5 cut(s) 70, 73, 434, 699, 778
PleI GAGTC 2 cut(s) 415, 665
PpsI GAGTC 2 cut(s) 415, 665
PpuMI RGGWCCY 1 cut(s) 520
Psp5II RGGWCCY 1 cut(s) 520
Psp6I CCWGG 4 cut(s) 195, 226, 505, 700
PspGI CCWGG 4 cut(s) 195, 226, 505, 700
PspN4I GGNNCC 3 cut(s) 6, 346, 522
PspPI GGNCC 7 cut(s) 218, 459, 520, 533, 626, 682, 1073
PspPPI RGGWCCY 1 cut(s) 520
PstI CTGCAG 2 cut(s) 70, 406
PstNI CAGNNNCTG 1 cut(s) 424
PsyI GACNNNGTC 1 cut(s) 1088
PvuII CAGCTG 1 cut(s) 71
RsaI GTAC 2 cut(s) 206, 737
RsaNI GTAC 2 cut(s) 205, 736
RseI CAYNNNNRTG 2 cut(s) 87, 1068
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 5 cut(s) 69, 72, 433, 698, 777
Sau3AI GATC 3 cut(s) 564, 708, 893
Sau96I GGNCC 7 cut(s) 218, 459, 520, 533, 626, 682, 1073
SchI GAGTC 2 cut(s) 415, 666
ScrFI CCNGG 7 cut(s) 197, 228, 317, 318, 393, 507, 702
SduI GDGCHC 2 cut(s) 364, 378
SfaNI GCATC 2 cut(s) 535, 862
SfcI CTRYAG 2 cut(s) 66, 402
SfuI TTCGAA 1 cut(s) 114
SinI GGWCC 4 cut(s) 520, 533, 626, 682
SmaI CCCGGG 1 cut(s) 318
SmiMI CAYNNNNRTG 2 cut(s) 87, 1068
SmlI CTYRAG 3 cut(s) 363, 377, 951
SmoI CTYRAG 3 cut(s) 363, 377, 951
Sse9I AATT 5 cut(s) 595, 643, 780, 785, 819
SseBI AGGCCT 1 cut(s) 287
SsiI CCGC 3 cut(s) 525, 531, 695
SspMI CTAG 2 cut(s) 1011, 1107
StuI AGGCCT 1 cut(s) 287
StyD4I CCNGG 7 cut(s) 195, 226, 315, 316, 391, 505, 700
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 3 cut(s) 151, 626, 1028
TaiI ACGT 2 cut(s) 306, 497
TaqI TCGA 4 cut(s) 114, 189, 201, 714
TaqII GACCGA 1 cut(s) 313
TasI AATT 5 cut(s) 595, 643, 780, 785, 819
TatI WGTACW 2 cut(s) 204, 735
TfiI GAWTC 4 cut(s) 119, 511, 716, 771
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 2 cut(s) 58, 1033
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 5 cut(s) 68, 71, 432, 697, 776
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 1 cut(s) 1040
TspGWI ACGGA 1 cut(s) 376
TspMI CCCGGG 1 cut(s) 316
TspRI CASTG 2 cut(s) 58, 1033
Tth111I GACNNNGTC 1 cut(s) 1088
VpaK11BI GGWCC 4 cut(s) 520, 533, 626, 682
XagI CCTNNNNNAGG 1 cut(s) 225
XapI RAATTY 1 cut(s) 643
XmaI CCCGGG 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 327
XmnI GAANNNNTTC 1 cut(s) 752
XspI CTAG 2 cut(s) 1011, 1107
Zsp2I ATGCAT 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.