RLG00000023374

Protein of unknown function (DUF642)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011779
Physical Location & Seq
Reverse (-)
22718812 .. 22721115
2304 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023374

Sequence Viewer

Length: 1119 bp
ATGGCTCCTCCCTCTTTCTTCTTTCTTTGCTTTCTGGCCATTATCGTTCGCTGCACTGCTTACGCTTCTGCAGCTGCTACTCTACATCTTGATGGGGAACTACCAAATGGGCACTTCGAAGAATCACCCAGTTCCTCAAACCTAAAGAAAACAGTCATCGTCGGGAAACACTCACTCCCAAAGTGGGAAATCGATGGCCTGGTCGAGTACATCGCAGGTGGGCCTCAGCCAGGTGGCTTCTACTTTCCGGTGGCGCGTGGGGTCCACGCCGTGAGGCTCGGCAACGAGGCCTCCATTTCTCAGAACGTCAATGTCAACCCGGGTTCGGTCTACTCTCTCACTTTTGGCACCACAAGAACTTGTGCTCAAGACGAGGTGCTCAAGGTCTCGGTGCCGGGTCAGTCTGCAGAGCTTTCCATACAGACTCTGTTCAGCAGCGATGGGGGTGACACTTATGCATGGGCCTTTAAGGCAACTTCCAAGGTTGTTAGGGTTACGTTCCACAACCCTGGGATTCAAGAGGACCCCGCTTGCGGTCCTCTCTTGGATGCTATTGCAATCAAGGAGATCATACTTCCTCAGAAGTATATAAGAGGTAATTTAGTCAAAAATGGTGGCTTTGAAAACGGTCCTCATGTATTCAAGAATTTCTCAACCGGAGTCCTCCTCCCTCCAAAGTTACTGGACCAAATCTCACCGTTGCCCGGATGGATCATCGAATCCCTAAAGCCTGTGAAGTACATTGACAGGAAGCACTTCTCAGTTCCAGTCGGATTCGCAGCAATTGAATTGGTTGCAGGAAGGGAGAGTGCCATTGCTCAAATTATTAGAACAATCCCAAACAAATCCTACAACCTTACCTTCACCATTGGGGATGCAAAGAATGGTTGTCATGGATCAATGATGGTTGAAGCCTTCGCTGGTAAGGAAACCCTAAAAGTTCCTTATGTGTCTCAAGGCAAGGGTGGATATAGAACTGCAAGCTTCAAGTTCCAAGCAGTTTCCCCAAGAACTAGAATAACATTTTACAGTGCATATTATCACACAAAGCTTCATGATTTTGGACATATGTGTGGCCCCGTCTTAGACAATGTCATTGTCTTTCCAACTAGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF642

Protein Analysis

373

Amino Acids

40.45

Weight (kDa)

8.97

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF642 PF04862 33 - 188 2e-63 Protein of unknown function (DUF642)
DUF642 PF04862 201 - 367 4.2e-15 Protein of unknown function (DUF642)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011779)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41800 AT2G41810
fragaria_vesca FvH4_6g23670
malus_domestica MD14G1071300.v1.1
prunus_persica Prupe.7G061700_v2.0.a1
pyrus_communis pycom14g06080
rosa_chinensis RchiOBHm_Chr3g0482101
rosa_laevigata RLG00000023374
rosa_multiflora Rmu_co8504877.1_g000001 Rmu_sc0000192.1_g000028 Rmu_sc0001738.1_g000034
rosa_roxburghii Rroxscaffold_6G00399700 Rroxscaffold_6G00400070
rosa_rugosa Rorug03G0194800
rosa_samantha Rh3AG246000 Rh3BG281100 Rh3CG277200 Rh3DG273500
rosa_wichuraiana Rw3G022000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 206
Acc36I ACCTGC 1 cut(s) 206
AccB1I GGYRCC 2 cut(s) 347, 391
AccI GTMKAC 1 cut(s) 330
AccII CGCG 1 cut(s) 256
AciI CCGC 2 cut(s) 528, 534
AclWI GGATC 2 cut(s) 719, 904
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 646
AdeI CACNNNGTG 1 cut(s) 271
AfaI GTAC 2 cut(s) 209, 740
AfiI CCNNNNNNNGG 5 cut(s) 184, 230, 325, 533, 704
AgsI TTSAA 6 cut(s) 518, 623, 643, 788, 911, 988
AjnI CCWGG 3 cut(s) 198, 229, 508
AluBI AGCT 4 cut(s) 74, 412, 984, 1051
AluI AGCT 4 cut(s) 74, 412, 984, 1051
Alw21I GWGCWC 2 cut(s) 367, 381
Alw26I GTCTC 2 cut(s) 391, 957
AlwI GGATC 2 cut(s) 719, 904
AlwNI CAGNNNCTG 1 cut(s) 427
Ama87I CYCGRG 1 cut(s) 319
AoxI GGCC 6 cut(s) 36, 196, 221, 288, 462, 1075
ApeKI GCWGC 5 cut(s) 51, 71, 74, 435, 779
ApoI RAATTY 1 cut(s) 646
Asp700I GAANNNNTTC 1 cut(s) 755
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 8 cut(s) 221, 262, 462, 523, 536, 629, 685, 1076
AsuC2I CCSGG 4 cut(s) 320, 321, 396, 705
AsuHPI GGTGA 4 cut(s) 117, 458, 687, 856
AsuII TTCGAA 1 cut(s) 117
AvaI CYCGRG 1 cut(s) 319
AvaII GGWCC 5 cut(s) 262, 523, 536, 629, 685
BaeGI GKGCMC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 38
BanI GGYRCC 2 cut(s) 347, 391
Bbv12I GWGCWC 2 cut(s) 367, 381
BbvCI CCTCAGC 1 cut(s) 225
BbvI GCAGC 5 cut(s) 38, 61, 83, 447, 791
BccI CCATC 5 cut(s) 86, 188, 434, 702, 898
BceAI ACGGC 1 cut(s) 254
BciT130I CCWGG 3 cut(s) 200, 231, 510
BcnI CCSGG 4 cut(s) 320, 321, 396, 705
BcoDI GTCTC 2 cut(s) 391, 957
BfaI CTAG 2 cut(s) 1014, 1110
BfmI CTRYAG 2 cut(s) 69, 405
BfuAI ACCTGC 1 cut(s) 206
BglI GCCNNNNNGGC 1 cut(s) 470
BisI GCNGC 5 cut(s) 52, 72, 75, 436, 780
BlsI GCNGC 5 cut(s) 53, 73, 76, 437, 781
Bme1390I CCNGG 7 cut(s) 200, 231, 320, 321, 396, 510, 705
Bme18I GGWCC 5 cut(s) 262, 523, 536, 629, 685
BmeT110I CYCGRG 1 cut(s) 319
BmgT120I GGNCC 8 cut(s) 221, 262, 462, 523, 536, 629, 685, 1076
BmiI GGNNCC 6 cut(s) 6, 263, 349, 393, 525, 1078
BmrFI CCNGG 7 cut(s) 200, 231, 320, 321, 396, 510, 705
BmrI ACTGGG 1 cut(s) 123
BmsI GCATC 2 cut(s) 538, 865
BmuI ACTGGG 1 cut(s) 123
BplI GAGNNNNNCTC 2 cut(s) 651, 683
Bpu10I CCTNAGC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 117
BpuEI CTTGAG 3 cut(s) 351, 365, 939
BpuMI CCSGG 4 cut(s) 320, 321, 396, 705
Bsa29I ATCGAT 1 cut(s) 192
BsaI GGTCTC 1 cut(s) 391
BsaJI CCNNGG 4 cut(s) 319, 480, 508, 509
BsaWI WCCGGW 2 cut(s) 247, 656
BsaXI ACNNNNNCTCC 4 cut(s) 159, 189, 275, 305
Bsc4I CCNNNNNNNGG 5 cut(s) 184, 230, 325, 533, 704
Bse1I ACTGG 3 cut(s) 129, 687, 767
Bse3DI GCAATG 1 cut(s) 813
BseBI CCWGG 3 cut(s) 200, 231, 510
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 4 cut(s) 319, 480, 508, 509
BseGI GGATG 3 cut(s) 553, 713, 880
BseLI CCNNNNNNNGG 5 cut(s) 184, 230, 325, 533, 704
BseMI GCAATG 1 cut(s) 813
BseMII CTCAG 4 cut(s) 239, 314, 593, 774
BseNI ACTGG 3 cut(s) 129, 687, 767
BseRI GAGGAG 1 cut(s) 656
BseSI GKGCMC 1 cut(s) 114
BseXI GCAGC 5 cut(s) 38, 61, 83, 447, 791
BsgI GTGCAG 1 cut(s) 37
Bsh1236I CGCG 1 cut(s) 256
BshFI GGCC 6 cut(s) 38, 198, 223, 290, 464, 1077
BshNI GGYRCC 2 cut(s) 347, 391
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 2 cut(s) 367, 381
BsiHKCI CYCGRG 1 cut(s) 319
BsiSI CCGG 5 cut(s) 248, 320, 395, 657, 705
BslI CCNNNNNNNGG 5 cut(s) 184, 230, 325, 533, 704
BsmAI GTCTC 2 cut(s) 391, 957
BsnI GGCC 6 cut(s) 38, 198, 223, 290, 464, 1077
Bso31I GGTCTC 1 cut(s) 391
BsoBI CYCGRG 1 cut(s) 319
Bsp119I TTCGAA 1 cut(s) 117
Bsp1286I GDGCHC 3 cut(s) 114, 367, 381
Bsp143I GATC 3 cut(s) 567, 711, 896
BspACI CCGC 2 cut(s) 528, 534
BspANI GGCC 6 cut(s) 38, 198, 223, 290, 464, 1077
BspCNI CTCAG 4 cut(s) 238, 313, 592, 773
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 1 cut(s) 256
BspHI TCATGA 1 cut(s) 1054
BspLI GGNNCC 6 cut(s) 6, 263, 349, 393, 525, 1078
BspMAI CTGCAG 2 cut(s) 73, 409
BspMI ACCTGC 1 cut(s) 206
BspPI GGATC 2 cut(s) 719, 904
BspT104I TTCGAA 1 cut(s) 117
BspT107I GGYRCC 2 cut(s) 347, 391
BspTNI GGTCTC 1 cut(s) 391
BsrDI GCAATG 1 cut(s) 813
BsrI ACTGG 3 cut(s) 129, 687, 767
BssECI CCNNGG 4 cut(s) 319, 480, 508, 509
BssMI GATC 3 cut(s) 567, 711, 896
BssT1I CCWWGG 1 cut(s) 480
Bst2UI CCWGG 3 cut(s) 200, 231, 510
Bst4CI ACNGT 4 cut(s) 154, 629, 699, 1031
BstBI TTCGAA 1 cut(s) 117
BstC8I GCNNGC 2 cut(s) 532, 982
BstDEI CTNAG 5 cut(s) 225, 300, 579, 760, 1084
BstENI CCTNNNNNAGG 1 cut(s) 228
BstF5I GGATG 3 cut(s) 553, 713, 880
BstFNI CGCG 1 cut(s) 256
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 3 cut(s) 570, 714, 899
BstMAI GTCTC 2 cut(s) 391, 957
BstMBI GATC 3 cut(s) 567, 711, 896
BstMWI GCNNNNNNNGC 2 cut(s) 71, 470
BstNI CCWGG 3 cut(s) 200, 231, 510
BstSCI CCNGG 7 cut(s) 198, 229, 318, 319, 394, 508, 703
BstSFI CTRYAG 2 cut(s) 69, 405
BstSLI GKGCMC 1 cut(s) 114
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 5 cut(s) 38, 61, 83, 447, 791
BstXI CCANNNNNNTGG 1 cut(s) 509
Bsu15I ATCGAT 1 cut(s) 192
BsuRI GGCC 6 cut(s) 38, 198, 223, 290, 464, 1077
BsuTUI ATCGAT 1 cut(s) 192
BtgZI GCGATG 2 cut(s) 196, 453
BtsCI GGATG 3 cut(s) 553, 713, 880
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 54, 1036
BveI ACCTGC 1 cut(s) 206
Cac8I GCNNGC 2 cut(s) 532, 982
CaiI CAGNNNCTG 1 cut(s) 427
CciI TCATGA 1 cut(s) 1054
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 8 cut(s) 221, 262, 462, 523, 536, 629, 685, 1076
Cfr9I CCCGGG 1 cut(s) 319
ClaI ATCGAT 1 cut(s) 192
Csp6I GTAC 2 cut(s) 208, 739
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
CviAII CATG 4 cut(s) 459, 635, 893, 1055
CviQI GTAC 2 cut(s) 208, 739
DdeI CTNAG 5 cut(s) 225, 300, 579, 760, 1084
DpnI GATC 3 cut(s) 569, 713, 898
DpnII GATC 3 cut(s) 567, 711, 896
DraIII CACNNNGTG 1 cut(s) 271
EaeI YGGCCR 1 cut(s) 36
Eco130I CCWWGG 1 cut(s) 480
Eco147I AGGCCT 1 cut(s) 290
Eco31I GGTCTC 1 cut(s) 391
Eco47I GGWCC 5 cut(s) 262, 523, 536, 629, 685
Eco88I CYCGRG 1 cut(s) 319
EcoNI CCTNNNNNAGG 1 cut(s) 228
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 3 cut(s) 198, 229, 508
EcoT14I CCWWGG 1 cut(s) 480
EcoT22I ATGCAT 1 cut(s) 460
ErhI CCWWGG 1 cut(s) 480
FaeI CATG 4 cut(s) 462, 638, 896, 1058
FatI CATG 4 cut(s) 458, 634, 892, 1054
FauI CCCGC 1 cut(s) 535
FauNDI CATATG 1 cut(s) 1068
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 5 cut(s) 52, 72, 75, 436, 780
FokI GGATG 3 cut(s) 560, 720, 887
Fsp4HI GCNGC 5 cut(s) 52, 72, 75, 436, 780
FspBI CTAG 2 cut(s) 1014, 1110
GlaI GCGC 1 cut(s) 255
GluI GCNGC 5 cut(s) 52, 72, 75, 436, 780
HaeIII GGCC 6 cut(s) 38, 198, 223, 290, 464, 1077
HapII CCGG 5 cut(s) 248, 320, 395, 657, 705
HhaI GCGC 1 cut(s) 256
Hin1II CATG 4 cut(s) 462, 638, 896, 1058
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HindIII AAGCTT 2 cut(s) 982, 1049
HinfI GANTC 6 cut(s) 122, 424, 514, 660, 719, 774
HpaII CCGG 5 cut(s) 248, 320, 395, 657, 705
HphI GGTGA 4 cut(s) 117, 458, 687, 856
Hpy166II GTNNAC 3 cut(s) 265, 316, 331
Hpy188I TCNGA 3 cut(s) 303, 582, 773
Hpy188III TCNNGA 6 cut(s) 89, 163, 368, 518, 643, 1055
Hpy8I GTNNAC 3 cut(s) 265, 316, 331
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 3 cut(s) 795, 871, 925
HpyCH4III ACNGT 4 cut(s) 154, 629, 699, 1031
HpyCH4IV ACGT 2 cut(s) 306, 497
HpyCH4V TGCA 9 cut(s) 54, 71, 407, 458, 557, 797, 878, 980, 1034
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 470
HpyF3I CTNAG 5 cut(s) 225, 300, 579, 760, 1084
HpySE526I ACGT 2 cut(s) 306, 497
Hsp92II CATG 4 cut(s) 462, 638, 896, 1058
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 3 cut(s) 567, 711, 896
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 5 cut(s) 38, 61, 83, 447, 791
LweI GCATC 2 cut(s) 538, 865
MaeI CTAG 2 cut(s) 1014, 1110
MaeII ACGT 2 cut(s) 306, 497
MaeIII GTNAC 3 cut(s) 446, 493, 678
MalI GATC 3 cut(s) 569, 713, 898
MboI GATC 3 cut(s) 567, 711, 896
MboII GAAGA 2 cut(s) 10, 131
MfeI CAATTG 1 cut(s) 783
MhlI GDGCHC 3 cut(s) 114, 367, 381
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 5 cut(s) 598, 646, 783, 788, 822
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 2 cut(s) 418, 669
MmeI TCCRAC 1 cut(s) 751
Mox20I TGGCCA 1 cut(s) 38
Mph1103I ATGCAT 1 cut(s) 460
MroXI GAANNNNTTC 1 cut(s) 755
MscI TGGCCA 1 cut(s) 38
MseI TTAA 1 cut(s) 468
MslI CAYNNNNRTG 2 cut(s) 90, 1071
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 74
MspI CCGG 5 cut(s) 248, 320, 395, 657, 705
MspR9I CCNGG 7 cut(s) 200, 231, 320, 321, 396, 510, 705
MunI CAATTG 1 cut(s) 783
MvaI CCWGG 3 cut(s) 200, 231, 510
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 2 cut(s) 71, 470
NciI CCSGG 4 cut(s) 320, 321, 396, 705
NdeI CATATG 1 cut(s) 1068
NdeII GATC 3 cut(s) 567, 711, 896
NlaIII CATG 4 cut(s) 462, 638, 896, 1058
NlaIV GGNNCC 6 cut(s) 6, 263, 349, 393, 525, 1078
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 1 cut(s) 446
NsiI ATGCAT 1 cut(s) 460
NspV TTCGAA 1 cut(s) 117
PagI TCATGA 1 cut(s) 1054
PaqCI CACCTGC 1 cut(s) 206
PasI CCCWGGG 1 cut(s) 509
PceI AGGCCT 1 cut(s) 290
PdmI GAANNNNTTC 1 cut(s) 755
PfeI GAWTC 4 cut(s) 122, 514, 719, 774
PflFI GACNNNGTC 1 cut(s) 1091
PkrI GCNGC 5 cut(s) 53, 73, 76, 437, 781
PleI GAGTC 2 cut(s) 418, 668
PpsI GAGTC 2 cut(s) 418, 668
PpuMI RGGWCCY 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 3 cut(s) 198, 229, 508
PspGI CCWGG 3 cut(s) 198, 229, 508
PspN4I GGNNCC 6 cut(s) 6, 263, 349, 393, 525, 1078
PspPI GGNCC 8 cut(s) 221, 262, 462, 523, 536, 629, 685, 1076
PspPPI RGGWCCY 1 cut(s) 523
PstI CTGCAG 2 cut(s) 73, 409
PstNI CAGNNNCTG 1 cut(s) 427
PsyI GACNNNGTC 1 cut(s) 1091
PvuII CAGCTG 1 cut(s) 74
RsaI GTAC 2 cut(s) 209, 740
RsaNI GTAC 2 cut(s) 208, 739
RseI CAYNNNNRTG 2 cut(s) 90, 1071
SaqAI TTAA 1 cut(s) 468
SatI GCNGC 5 cut(s) 52, 72, 75, 436, 780
Sau3AI GATC 3 cut(s) 567, 711, 896
Sau96I GGNCC 8 cut(s) 221, 262, 462, 523, 536, 629, 685, 1076
SchI GAGTC 2 cut(s) 418, 669
ScrFI CCNGG 7 cut(s) 200, 231, 320, 321, 396, 510, 705
SduI GDGCHC 3 cut(s) 114, 367, 381
SfaNI GCATC 2 cut(s) 538, 865
SfcI CTRYAG 2 cut(s) 69, 405
SfuI TTCGAA 1 cut(s) 117
SinI GGWCC 5 cut(s) 262, 523, 536, 629, 685
SmaI CCCGGG 1 cut(s) 321
SmiMI CAYNNNNRTG 2 cut(s) 90, 1071
SmlI CTYRAG 3 cut(s) 366, 380, 954
SmoI CTYRAG 3 cut(s) 366, 380, 954
Sse9I AATT 5 cut(s) 598, 646, 783, 788, 822
SseBI AGGCCT 1 cut(s) 290
SsiI CCGC 2 cut(s) 528, 534
SspMI CTAG 2 cut(s) 1014, 1110
StuI AGGCCT 1 cut(s) 290
StyD4I CCNGG 7 cut(s) 198, 229, 318, 319, 394, 508, 703
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 4 cut(s) 154, 629, 699, 1031
TaiI ACGT 2 cut(s) 309, 500
TaqI TCGA 4 cut(s) 117, 192, 204, 717
TaqII GACCGA 1 cut(s) 316
TasI AATT 5 cut(s) 598, 646, 783, 788, 822
TatI WGTACW 2 cut(s) 207, 738
TfiI GAWTC 4 cut(s) 122, 514, 719, 774
Tru1I TTAA 1 cut(s) 468
Tru9I TTAA 1 cut(s) 468
TscAI CASTG 2 cut(s) 61, 1036
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 5 cut(s) 51, 71, 74, 435, 779
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 1 cut(s) 1043
TspMI CCCGGG 1 cut(s) 319
TspRI CASTG 2 cut(s) 61, 1036
Tth111I GACNNNGTC 1 cut(s) 1091
VpaK11BI GGWCC 5 cut(s) 262, 523, 536, 629, 685
XagI CCTNNNNNAGG 1 cut(s) 228
XapI RAATTY 1 cut(s) 646
XmaI CCCGGG 1 cut(s) 319
XmiI GTMKAC 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 755
XspI CTAG 2 cut(s) 1014, 1110
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.