Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0012819

Physical Location & Seq

Genomic Coordinates Reverse (-)

8961980 .. 8965020

Gene Structure 3041 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1078600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGGGCTCCCCATTTTACAGCTTCCTCAAGCAGAACAACATTGTCTACCTCAAAATTTTCTTGATTTTGTTAATCATGGGGTGCATACCGGATAGAACAACCAACCTTTGTTCCAACCAACTCATTGCCACACCAGCCACTCCAACTCACTTAAATGTTACTTCATCTTTGGAGACACTAAGCCTAGACGGCTACTTTAGTTTCAACAACAACAATCACGCAGCAAAGGACTTTGGCAATACATACCAGTTCCTCCCACTAGCAGTCCTATATCCGAAATCAGTTTCCGATATCTCCTCAACGGTGAAACTTATTTTCAACATGGGTTCCGCTTCGGAGCTTACTGTTGCCGCCAGAGGCCACGGCCACTCCCTTCAAGGCCAAGCACAAGCTCATAGAGGTGTAGTCATCGACATGGAATCGCTCAAGGGATCGCAAATGCAGGTTCACACTGGAGAGCAACCTTATGTGGATGTTTCAGGTGGTGAGCTGTGGATAAATATTCTGCATGAGACTCTCAAACTTGGGTTGGCACCGAAATCTTGGACCGATTACCTTCATCTCACTGTCGGTGGGACTTTATCGTACGCCGGCATCAGCGGTCAGGCCTTCCGGCATGGTCCCCAGATCAATAACGTATACCAGCTGCAGGTTGTCACAGGAAAAGGAGAAGTAATTACCTGCTCAAAGAGCAAAAATCCAGACCTTTTCTATGGTGTTCTTGGAGGGCTAGGTCAGTTTGGCATAATTACACAGGCCAGAATTTCTCTTGAACCAGCACCAAAGATGGTGAAATGGATTAGAGTTTTATACTTAGATTTCTCATCATTTTCCAAAGACCAAGAGTATCTCATAGCATCTGAAAATTCATTCGATTATATCGAAGGATTTGTAATACTTAACAGAACAGGTCTTCTCAATAACTGGAGATCTTCTTTCAATCCTAAAGATCCAATGCAAGCCAGCCAATTCAATTCGGATGGAAAAACACTTTACTGCCTAGAAATGGCCAAATATTTTAACCCCGACGTAACGAATGTCATAGATCAGATAACTGAACGCTTACTGTTAAAATTGCACTATATCCCATCCACCCTCTTCATGTCCGAAGTTTCGTTTGTCGAATTCCTAGATAGAGTGCATTTGTCGGAGCTAAAACTCCGAGCGAAAGGACTATGGGAAGTTCCCCACCCGTGGATGAACCTACTCATTCCGAAAAACAGAATACACGAGTTTGCAGATGAGGTCTTTGGTAACGTTCTCACAAACAATATCAATGGTCCTATCCTTGTGTACCCAGTCAACAAAACCAAGTGGAACAACAAAACATCTTTGGTAACCCCAGATGAAGATGTATTCTACCTAGTGGCATTGCTATCATCTGCAGTGCCATCTTCCAAAGGAACAGACGGCCTAGAATACATTCTAACACAAAATAAAAGAATACTAGAATACAGCGCTAGAGCCGACCTTGGTATCAAGCAATATCTTCCTCATTACAGAACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009308 GO:0009690 GO:0009823 GO:0009888 GO:0009987 GO:0010817 GO:0016491 GO:0016645 GO:0019139 GO:0032502 GO:0034754 GO:0042445 GO:0042447 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0048507 GO:0048856 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko00908 map00908

Pfam Domains

Protein Families

Cytokin-bind FAD_binding_4

Protein Analysis

503

Amino Acids

56.22

Weight (kDa)

6.92

Isoelectric Point (pI)

30.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	86 - 229	4.1e-18	FAD binding domain
Cytokin-bind	PF09265	261 - 502	2.3e-94	Cytokinin dehydrogenase 1, FAD and cytokinin binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012819)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41510 AT2G41510 AT2G41510
fragaria_vesca	FvH4_6g24620
malus_domestica	MD12G1083600.v1.1 MD14G1078600.v1.1
prunus_persica	Prupe.7G052300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0483951
rosa_laevigata	RLG00000023246
rosa_multiflora	Rmu_sc0003477.1_g000016
rosa_roxburghii	Rroxscaffold_6G00397930
rosa_rugosa	Rorug03G0209600 Rorug03G0209700
rosa_samantha	Rh3AG257900 Rh3BG295000 Rh3CG292400 Rh3DG288000
rosa_wichuraiana	Rw3G023300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	3 cut(s)	433, 640, 689
AccB1I	GGYRCC	1 cut(s)	532
AccI	GTMKAC	2 cut(s)	45, 639
AciI	CCGC	3 cut(s)	330, 351, 600
AclI	AACGTT	1 cut(s)	1257
AclWI	GGATC	2 cut(s)	439, 944
AcoI	YGGCCR	2 cut(s)	364, 1008
AcsI	RAATTY	4 cut(s)	54, 762, 865, 1124
AfaI	GTAC	2 cut(s)	587, 1295
AfeI	AGCGCT	1 cut(s)	1459
AfiI	CCNNNNNNNGG	3 cut(s)	649, 1006, 1194
AgsI	TTSAA	6 cut(s)	205, 319, 377, 773, 940, 973
AluBI	AGCT	6 cut(s)	21, 340, 392, 490, 646, 1153
AluI	AGCT	6 cut(s)	21, 340, 392, 490, 646, 1153
Alw26I	GTCTC	2 cut(s)	167, 506
AlwI	GGATC	2 cut(s)	439, 944
Aor51HI	AGCGCT	1 cut(s)	1459
AoxI	GGCC	7 cut(s)	358, 364, 379, 606, 756, 1008, 1411
ApeKI	GCWGC	2 cut(s)	221, 646
ApoI	RAATTY	4 cut(s)	54, 762, 865, 1124
Asp700I	GAANNNNTTC	1 cut(s)	1422
AspLEI	GCGC	1 cut(s)	1460
AspS9I	GGNCC	3 cut(s)	546, 620, 1280
AsuHPI	GGTGA	3 cut(s)	316, 497, 802
AvaII	GGWCC	3 cut(s)	546, 620, 1280
BaeI	ACNNNNGTAYC	2 cut(s)	1459, 1492
BalI	TGGCCA	1 cut(s)	1010
BanI	GGYRCC	1 cut(s)	532
BanII	GRGCYC	1 cut(s)	8
BauI	CACGAG	1 cut(s)	1229
BbsI	GAAGAC	1 cut(s)	905
BbvI	GCAGC	2 cut(s)	233, 633
BccI	CCATC	4 cut(s)	781, 974, 1096, 1399
BceAI	ACGGC	3 cut(s)	205, 379, 1426
BcoDI	GTCTC	2 cut(s)	167, 506
BfaI	CTAG	9 cut(s)	185, 260, 731, 1001, 1130, 1364, 1415, 1448, 1461
BfmI	CTRYAG	2 cut(s)	647, 1383
BfoI	RGCGCY	1 cut(s)	1461
BfuAI	ACCTGC	3 cut(s)	433, 640, 689
BglI	GCCNNNNNGGC	1 cut(s)	189
BglII	AGATCT	1 cut(s)	929
BisI	GCNGC	3 cut(s)	222, 351, 647
BlsI	GCNGC	3 cut(s)	223, 352, 648
Bme18I	GGWCC	3 cut(s)	546, 620, 1280
BmgT120I	GGNCC	3 cut(s)	546, 620, 1280
BmiI	GGNNCC	4 cut(s)	7, 328, 534, 622
BmrI	ACTGGG	1 cut(s)	1292
BmsI	GCATC	2 cut(s)	603, 866
BmuI	ACTGGG	1 cut(s)	1292
BpiI	GAAGAC	1 cut(s)	905
BpmI	CTGGAG	2 cut(s)	474, 946
BpuEI	CTTGAG	2 cut(s)	11, 410
BsaJI	CCNNGG	3 cut(s)	361, 1193, 1471
BsaWI	WCCGGW	1 cut(s)	88
BsaXI	ACNNNNNCTCC	2 cut(s)	353, 383
Bsc4I	CCNNNNNNNGG	3 cut(s)	649, 1006, 1194
Bse118I	RCCGGY	1 cut(s)	590
Bse1I	ACTGG	4 cut(s)	247, 457, 929, 1298
Bse3DI	GCAATG	2 cut(s)	123, 1370
BseDI	CCNNGG	3 cut(s)	361, 1193, 1471
BseGI	GGATG	4 cut(s)	478, 985, 1088, 1203
BseLI	CCNNNNNNNGG	3 cut(s)	649, 1006, 1194
BseMI	GCAATG	2 cut(s)	123, 1370
BseNI	ACTGG	4 cut(s)	247, 457, 929, 1298
BseRI	GAGGAG	1 cut(s)	286
BseXI	GCAGC	2 cut(s)	233, 633
BshFI	GGCC	7 cut(s)	360, 366, 381, 608, 758, 1010, 1413
BshNI	GGYRCC	1 cut(s)	532
BsiSI	CCGG	3 cut(s)	89, 591, 613
BsiWI	CGTACG	1 cut(s)	585
BslFI	GGGAC	2 cut(s)	589, 606
BslI	CCNNNNNNNGG	3 cut(s)	649, 1006, 1194
BsmAI	GTCTC	2 cut(s)	167, 506
BsmFI	GGGAC	2 cut(s)	589, 606
BsnI	GGCC	7 cut(s)	360, 366, 381, 608, 758, 1010, 1413
Bsp1286I	GDGCHC	1 cut(s)	8
Bsp143I	GATC	5 cut(s)	431, 627, 929, 949, 1045
BspACI	CCGC	3 cut(s)	330, 351, 600
BspANI	GGCC	7 cut(s)	360, 366, 381, 608, 758, 1010, 1413
BspLI	GGNNCC	4 cut(s)	7, 328, 534, 622
BspMAI	CTGCAG	2 cut(s)	651, 1387
BspMI	ACCTGC	3 cut(s)	433, 640, 689
BspPI	GGATC	2 cut(s)	439, 944
BspT107I	GGYRCC	1 cut(s)	532
BsrDI	GCAATG	2 cut(s)	123, 1370
BsrFI	RCCGGY	1 cut(s)	590
BsrI	ACTGG	4 cut(s)	247, 457, 929, 1298
BssAI	RCCGGY	1 cut(s)	590
BssECI	CCNNGG	3 cut(s)	361, 1193, 1471
BssMI	GATC	5 cut(s)	431, 627, 929, 949, 1045
BssNAI	GTATAC	1 cut(s)	640
BssSI	CACGAG	1 cut(s)	1229
BssT1I	CCWWGG	1 cut(s)	1471
Bst1107I	GTATAC	1 cut(s)	640
Bst2BI	CACGAG	1 cut(s)	1229
Bst4CI	ACNGT	4 cut(s)	304, 346, 568, 1068
Bst6I	CTCTTC	1 cut(s)	1103
BstC8I	GCNNGC	3 cut(s)	592, 960, 964
BstDEI	CTNAG	2 cut(s)	179, 814
BstDSI	CCRYGG	2 cut(s)	361, 1193
BstEII	GGTNACC	1 cut(s)	1336
BstF5I	GGATG	4 cut(s)	478, 985, 1088, 1203
BstH2I	RGCGCY	1 cut(s)	1461
BstHHI	GCGC	1 cut(s)	1460
BstKTI	GATC	5 cut(s)	434, 630, 932, 952, 1048
BstMAI	GTCTC	2 cut(s)	167, 506
BstMBI	GATC	5 cut(s)	431, 627, 929, 949, 1045
BstMWI	GCNNNNNNNGC	3 cut(s)	134, 189, 690
BstPI	GGTNACC	1 cut(s)	1336
BstSFI	CTRYAG	2 cut(s)	647, 1383
BstV1I	GCAGC	2 cut(s)	233, 633
BstV2I	GAAGAC	1 cut(s)	905
BstX2I	RGATCY	2 cut(s)	929, 949
BstYI	RGATCY	2 cut(s)	929, 949
BstZ17I	GTATAC	1 cut(s)	640
BsuRI	GGCC	7 cut(s)	360, 366, 381, 608, 758, 1010, 1413
BtgI	CCRYGG	2 cut(s)	361, 1193
BtsCI	GGATG	4 cut(s)	478, 985, 1088, 1203
BtsI	GCAGTG	1 cut(s)	1392
BtsIMutI	CAGTG	3 cut(s)	450, 564, 1392
BveI	ACCTGC	3 cut(s)	433, 640, 689
Cac8I	GCNNGC	3 cut(s)	592, 960, 964
CfoI	GCGC	1 cut(s)	1460
Cfr10I	RCCGGY	1 cut(s)	590
Cfr13I	GGNCC	3 cut(s)	546, 620, 1280
Csp6I	GTAC	2 cut(s)	586, 1294
CviAII	CATG	6 cut(s)	76, 322, 415, 509, 617, 1102
CviQI	GTAC	2 cut(s)	586, 1294
DdeI	CTNAG	2 cut(s)	179, 814
DpnI	GATC	5 cut(s)	433, 629, 931, 951, 1047
DpnII	GATC	5 cut(s)	431, 627, 929, 949, 1045
EaeI	YGGCCR	2 cut(s)	364, 1008
Eam1104I	CTCTTC	1 cut(s)	1103
EarI	CTCTTC	1 cut(s)	1103
Eco130I	CCWWGG	1 cut(s)	1471
Eco147I	AGGCCT	1 cut(s)	608
Eco24I	GRGCYC	1 cut(s)	8
Eco32I	GATATC	1 cut(s)	292
Eco47I	GGWCC	3 cut(s)	546, 620, 1280
Eco47III	AGCGCT	1 cut(s)	1459
Eco91I	GGTNACC	1 cut(s)	1336
EcoO65I	GGTNACC	1 cut(s)	1336
EcoRI	GAATTC	1 cut(s)	1124
EcoRV	GATATC	1 cut(s)	292
EcoT14I	CCWWGG	1 cut(s)	1471
EcoT38I	GRGCYC	1 cut(s)	8
ErhI	CCWWGG	1 cut(s)	1471
FaeI	CATG	6 cut(s)	79, 325, 418, 512, 620, 1105
FaqI	GGGAC	2 cut(s)	589, 606
FatI	CATG	6 cut(s)	75, 321, 414, 508, 616, 1101
FblI	GTMKAC	2 cut(s)	45, 639
Fnu4HI	GCNGC	3 cut(s)	222, 351, 647
FokI	GGATG	4 cut(s)	485, 992, 1075, 1210
FriOI	GRGCYC	1 cut(s)	8
Fsp4HI	GCNGC	3 cut(s)	222, 351, 647
FspBI	CTAG	9 cut(s)	185, 260, 731, 1001, 1130, 1364, 1415, 1448, 1461
GlaI	GCGC	1 cut(s)	1459
GluI	GCNGC	3 cut(s)	222, 351, 647
GsuI	CTGGAG	2 cut(s)	474, 946
HaeII	RGCGCY	1 cut(s)	1461
HaeIII	GGCC	7 cut(s)	360, 366, 381, 608, 758, 1010, 1413
HapII	CCGG	3 cut(s)	89, 591, 613
HhaI	GCGC	1 cut(s)	1460
Hin1II	CATG	6 cut(s)	79, 325, 418, 512, 620, 1105
Hin6I	GCGC	1 cut(s)	1458
HinP1I	GCGC	1 cut(s)	1458
HincII	GTYRAC	1 cut(s)	1303
HindII	GTYRAC	1 cut(s)	1303
HinfI	GANTC	2 cut(s)	419, 514
HpaII	CCGG	3 cut(s)	89, 591, 613
HphI	GGTGA	3 cut(s)	316, 497, 802
Hpy166II	GTNNAC	5 cut(s)	46, 448, 640, 1294, 1303
Hpy188III	TCNNGA	3 cut(s)	61, 701, 770
Hpy8I	GTNNAC	5 cut(s)	46, 448, 640, 1294, 1303
Hpy99I	CGWCG	1 cut(s)	1031
HpyAV	CCTTC	4 cut(s)	383, 566, 619, 878
HpyCH4III	ACNGT	4 cut(s)	304, 346, 568, 1068
HpyCH4IV	ACGT	3 cut(s)	636, 1029, 1257
HpyCH4V	TGCA	9 cut(s)	84, 442, 508, 649, 958, 1078, 1141, 1238, 1385
HpyF10VI	GCNNNNNNNGC	3 cut(s)	134, 189, 690
HpyF3I	CTNAG	2 cut(s)	179, 814
HpySE526I	ACGT	3 cut(s)	636, 1029, 1257
Hsp92II	CATG	6 cut(s)	79, 325, 418, 512, 620, 1105
HspAI	GCGC	1 cut(s)	1458
KroI	GCCGGC	1 cut(s)	590
KroNI	GCCGGC	1 cut(s)	592
Kzo9I	GATC	5 cut(s)	431, 627, 929, 949, 1045
LmnI	GCTCC	3 cut(s)	11, 337, 1150
Lsp1109I	GCAGC	2 cut(s)	233, 633
LweI	GCATC	2 cut(s)	603, 866
MaeI	CTAG	9 cut(s)	185, 260, 731, 1001, 1130, 1364, 1415, 1448, 1461
MaeII	ACGT	3 cut(s)	636, 1029, 1257
MaeIII	GTNAC	5 cut(s)	157, 655, 1030, 1253, 1336
MalI	GATC	5 cut(s)	433, 629, 931, 951, 1047
MboI	GATC	5 cut(s)	431, 627, 929, 949, 1045
MboII	GAAGA	6 cut(s)	905, 924, 1090, 1361, 1386, 1481
MflI	RGATCY	2 cut(s)	929, 949
MhlI	GDGCHC	1 cut(s)	8
MlsI	TGGCCA	1 cut(s)	1010
MluCI	AATT	9 cut(s)	54, 675, 747, 762, 865, 968, 973, 1073, 1124
MluNI	TGGCCA	1 cut(s)	1010
MlyI	GAGTC	1 cut(s)	508
MmeI	TCCRAC	3 cut(s)	138, 167, 1128
Mox20I	TGGCCA	1 cut(s)	1010
MroNI	GCCGGC	1 cut(s)	590
MroXI	GAANNNNTTC	1 cut(s)	1422
MscI	TGGCCA	1 cut(s)	1010
MseI	TTAA	5 cut(s)	71, 152, 900, 1020, 1070
MslI	CAYNNNNRTG	3 cut(s)	153, 399, 413
Msp20I	TGGCCA	1 cut(s)	1010
MspA1I	CMGCKG	2 cut(s)	600, 646
MspI	CCGG	3 cut(s)	89, 591, 613
MwoI	GCNNNNNNNGC	3 cut(s)	134, 189, 690
NaeI	GCCGGC	1 cut(s)	592
NdeII	GATC	5 cut(s)	431, 627, 929, 949, 1045
NgoMIV	GCCGGC	1 cut(s)	590
NlaIII	CATG	6 cut(s)	79, 325, 418, 512, 620, 1105
NlaIV	GGNNCC	4 cut(s)	7, 328, 534, 622
NmuCI	GTSAC	1 cut(s)	655
PceI	AGGCCT	1 cut(s)	608
PcsI	WCGNNNNNNNCGW	1 cut(s)	879
PdiI	GCCGGC	1 cut(s)	592
PdmI	GAANNNNTTC	1 cut(s)	1422
PfeI	GAWTC	1 cut(s)	419
Pfl23II	CGTACG	1 cut(s)	585
PkrI	GCNGC	3 cut(s)	223, 352, 648
PleI	GAGTC	1 cut(s)	508
PpsI	GAGTC	1 cut(s)	508
Psp1406I	AACGTT	1 cut(s)	1257
PspEI	GGTNACC	1 cut(s)	1336
PspLI	CGTACG	1 cut(s)	585
PspN4I	GGNNCC	4 cut(s)	7, 328, 534, 622
PspPI	GGNCC	3 cut(s)	546, 620, 1280
PstI	CTGCAG	2 cut(s)	651, 1387
PsuI	RGATCY	2 cut(s)	929, 949
PvuII	CAGCTG	1 cut(s)	646
RsaI	GTAC	2 cut(s)	587, 1295
RsaNI	GTAC	2 cut(s)	586, 1294
RseI	CAYNNNNRTG	3 cut(s)	153, 399, 413
SaqAI	TTAA	5 cut(s)	71, 152, 900, 1020, 1070
SatI	GCNGC	3 cut(s)	222, 351, 647
Sau3AI	GATC	5 cut(s)	431, 627, 929, 949, 1045
Sau96I	GGNCC	3 cut(s)	546, 620, 1280
SchI	GAGTC	1 cut(s)	508
SduI	GDGCHC	1 cut(s)	8
SfaNI	GCATC	2 cut(s)	603, 866
SfcI	CTRYAG	2 cut(s)	647, 1383
SinI	GGWCC	3 cut(s)	546, 620, 1280
SmiMI	CAYNNNNRTG	3 cut(s)	153, 399, 413
SmlI	CTYRAG	2 cut(s)	26, 425
SmoI	CTYRAG	2 cut(s)	26, 425
Sse9I	AATT	9 cut(s)	54, 675, 747, 762, 865, 968, 973, 1073, 1124
SseBI	AGGCCT	1 cut(s)	608
SsiI	CCGC	3 cut(s)	330, 351, 600
SspI	AATATT	2 cut(s)	502, 1016
SspMI	CTAG	9 cut(s)	185, 260, 731, 1001, 1130, 1364, 1415, 1448, 1461
StuI	AGGCCT	1 cut(s)	608
StyI	CCWWGG	1 cut(s)	1471
TaaI	ACNGT	4 cut(s)	304, 346, 568, 1068
TaiI	ACGT	3 cut(s)	639, 1032, 1260
TaqI	TCGA	4 cut(s)	411, 873, 882, 1122
TaqII	GACCGA	1 cut(s)	563
TasI	AATT	9 cut(s)	54, 675, 747, 762, 865, 968, 973, 1073, 1124
TauI	GCSGC	1 cut(s)	353
TfiI	GAWTC	1 cut(s)	419
Tru1I	TTAA	5 cut(s)	71, 152, 900, 1020, 1070
Tru9I	TTAA	5 cut(s)	71, 152, 900, 1020, 1070
TscAI	CASTG	3 cut(s)	457, 571, 1392
TseFI	GTSAC	1 cut(s)	655
TseI	GCWGC	2 cut(s)	221, 646
Tsp45I	GTSAC	1 cut(s)	655
TspDTI	ATGAA	6 cut(s)	153, 548, 858, 1090, 1214, 1362
TspRI	CASTG	3 cut(s)	457, 571, 1392
VpaK11BI	GGWCC	3 cut(s)	546, 620, 1280
XapI	RAATTY	4 cut(s)	54, 762, 865, 1124
XmiI	GTMKAC	2 cut(s)	45, 639
XmnI	GAANNNNTTC	1 cut(s)	1422
XspI	CTAG	9 cut(s)	185, 260, 731, 1001, 1130, 1364, 1415, 1448, 1461

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.