RchiOBHm_Chr3g0483951

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0012819
Physical Location & Seq
Reverse (-)
30340192 .. 30343233
3042 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44868

Sequence Viewer

Length: 1644 bp
ATGGACTCACCTCCATACAGCTTCCACAAACAGAACAACATGGTCTACCTCAAACTTTGCTTGATTTTATTCATGGGTTGTGTACCAGATACAACCAACCTCTGTTCCAACCAATTTCTGGCCACCCCAACCATTATTCCATACCGCTTAGATCCCTCTCATATCACTTCACCTTTCCAAACACTAAGCCTTGATGGTTACTTGAGTTTTGACAACAACCATCATACAGCAAAGGACTTTGGCAACATATACCATTTCCAACCGTCAGCAGTACTATATCCAAAATCTGTTTTTGATATCTCCTGCATAATAAAGCTTATATTTGAAATGGGTTCTGGTTCTGAGCTCACAGTTGCTGCCAGAGGACATGGTCACTCCCTTCAAGGTCAAGCTCAAGCTCATCGAGGCATAGTCATCAGCATGGAATCACTTGAGGGATCTCCAAAAATGCAGGTTCATATTGGAAAGCGACCTTATGTGGATGTTTCGGGTGGTGAGTTATGGATAAATATTCTGCATGAGACACTCAAACATGGGTTGGCACCAAAATCTTGGACAGATTACCTTCACCTCACCGTTGGCGGGACTTTGTCAAATGCGGGAATCAGTGGCCAGGCATTCCGGCATGGACCCCAGATCAACAATGTCTATCAGCTGGAGGTTGTCACAGGAAAAGGAGACATAATTACCTGTTCCGAAAAGAAAAATGCAGACCTTTTCTATGCTGTTTTGGGAGGGCTGGGACAGTTTGGCATCATTACAAAGGCTAGAATTTCTCTTCAATCGGCACCAAAGATGGTTAAGTGGATCAGAGTGCTGTACTCAGACTTCTCATTATTTTCAAAGGACCAAGAGCTTCTCATATCATCTGCAAATTCATTTGACTACATTGAAGGATTTGTTATAATTAACAGAACAGGTCTTCTTAATAGCTGGAGGTCTTCCTTCAATCCTAAAGATCCAATTCAAGCGAGCCAATTTAGCTCTGATGGTAAAACACTCTACTGCCTAGAAATGGCCAAATACTATAACCCAGACGAAACTGATGTCATGGATCAGAGAACTGAGATCTTACTGTCAGGATTACAATATATCCCTTCCACCCTCTTCACGTCAGAAGTTTCCTTTGTAAACTTCCTAGATAGAGTTCATTTGTCTGAGAGAAAACTACGAGAAAAAGGATTATGGGATGTTCCCCACCCGTGGATAAACCTTCTCATCCCTAAAAGCAAGATACATGAGTTTGCTGAAGAGGTCTTCGGCAAGATTCTCACAGACAATATCAATGGTCCTATCCTCATGTACCCAGTCAACAAAACCAAGTGGAACAACAACACATCTTTGGTTACCCCAGATGAAGATGTATTCTACCTAGTTGCATTCTTGTCCTCTGTGGTGCCATCTTCCACAGGAACAGATGGCTTTGACAAAATTTTAACACTAAACAGAAGAATCTTACAGTACTCTGCTACAGCTAACCTGGGTATCAAGCAATATCTGCCCCATTACAGAACCCAAGGAGAGTGGCGAGCTCATTTTGGCTCTCGATGGAAAGTTTTCCTACAGAGGAAGTCTTCTTATGACCCTTTGGCAATCCTTGCTCCTGGGCAGAGAATCTTTAAAAAGGCGGCTCCCATCCTATGA

Protein Analysis

547

Amino Acids

61.53

Weight (kDa)

8.5

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 88 - 232 7.6e-17 FAD binding domain
Cytokin-bind PF09265 264 - 540 2.5e-115 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41510 AT2G41510 AT2G41510
fragaria_vesca FvH4_6g24620
malus_domestica MD12G1083600.v1.1 MD14G1078600.v1.1
prunus_persica Prupe.7G052300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0483951
rosa_laevigata RLG00000023246
rosa_multiflora Rmu_sc0003477.1_g000016
rosa_roxburghii Rroxscaffold_6G00397930
rosa_rugosa Rorug03G0209600 Rorug03G0209700
rosa_samantha Rh3AG257900 Rh3BG295000 Rh3CG292400 Rh3DG288000
rosa_wichuraiana Rw3G023300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
Acc36I ACCTGC 1 cut(s) 442
AccB1I GGYRCC 3 cut(s) 541, 787, 1396
AccB7I CCANNNNNTGG 1 cut(s) 118
AccI GTMKAC 1 cut(s) 45
AciI CCGC 4 cut(s) 145, 582, 599, 1628
AclWI GGATC 5 cut(s) 146, 445, 815, 953, 1062
AcoI YGGCCR 3 cut(s) 120, 610, 1017
AcsI RAATTY 3 cut(s) 771, 874, 1431
AcuI CTGAAG 1 cut(s) 1269
AfaI GTAC 5 cut(s) 84, 273, 821, 1304, 1463
AfiI CCNNNNNNNGG 4 cut(s) 118, 582, 1015, 1203
AgsI TTSAA 7 cut(s) 326, 383, 782, 843, 893, 949, 968
AjiI CACGTC 1 cut(s) 1113
AjnI CCWGG 3 cut(s) 612, 1479, 1603
Alw21I GWGCWC 2 cut(s) 348, 1534
Alw26I GTCTC 2 cut(s) 515, 672
AlwI GGATC 5 cut(s) 146, 445, 815, 953, 1062
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 3 cut(s) 120, 610, 1017
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 3 cut(s) 771, 874, 1431
ArsI GACNNNNNNTTYG 2 cut(s) 1032, 1064
Asp700I GAANNNNTTC 1 cut(s) 1556
AspS9I GGNCC 3 cut(s) 629, 847, 1289
AsuHPI GGTGA 4 cut(s) 162, 506, 560, 565
AvaII GGWCC 3 cut(s) 629, 847, 1289
BaeI ACNNNNGTAYC 4 cut(s) 1226, 1259, 1468, 1501
BalI TGGCCA 3 cut(s) 122, 612, 1019
BanI GGYRCC 3 cut(s) 541, 787, 1396
BanII GRGCYC 2 cut(s) 348, 1534
BarI GAAGNNNNNNTAC 2 cut(s) 812, 844
BbsI GAAGAC 4 cut(s) 914, 933, 1249, 1566
Bbv12I GWGCWC 2 cut(s) 348, 1534
BbvI GCAGC 1 cut(s) 343
BccI CCATC 8 cut(s) 188, 228, 790, 983, 1408, 1412, 1542, 1643
BciT130I CCWGG 3 cut(s) 614, 1481, 1605
BcoDI GTCTC 2 cut(s) 515, 672
BfaI CTAG 4 cut(s) 768, 1010, 1139, 1373
BfmI CTRYAG 2 cut(s) 1470, 1562
BfuAI ACCTGC 1 cut(s) 442
BglII AGATCT 1 cut(s) 1068
BisI GCNGC 2 cut(s) 357, 1629
BlsI GCNGC 2 cut(s) 358, 1630
BmcAI AGTACT 2 cut(s) 273, 1463
Bme1390I CCNGG 3 cut(s) 614, 1481, 1605
Bme18I GGWCC 3 cut(s) 629, 847, 1289
BmgBI CACGTC 1 cut(s) 1113
BmgT120I GGNCC 3 cut(s) 629, 847, 1289
BmiI GGNNCC 5 cut(s) 543, 631, 789, 1398, 1632
BmrFI CCNGG 3 cut(s) 614, 1481, 1605
BmrI ACTGGG 1 cut(s) 1301
BmsI GCATC 1 cut(s) 762
BmuI ACTGGG 1 cut(s) 1301
BpiI GAAGAC 4 cut(s) 914, 933, 1249, 1566
BpmI CTGGAG 2 cut(s) 677, 955
BpuEI CTTGAG 3 cut(s) 223, 378, 452
BsaJI CCNNGG 4 cut(s) 1202, 1480, 1516, 1604
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 582, 1015, 1203
Bse1I ACTGG 1 cut(s) 1307
BseBI CCWGG 3 cut(s) 614, 1481, 1605
BseDI CCNNGG 4 cut(s) 1202, 1480, 1516, 1604
BseGI GGATG 4 cut(s) 487, 1195, 1218, 1635
BseLI CCNNNNNNNGG 4 cut(s) 118, 582, 1015, 1203
BseMII CTCAG 4 cut(s) 333, 837, 1056, 1149
BseNI ACTGG 1 cut(s) 1307
BseXI GCAGC 1 cut(s) 343
BseYI CCCAGC 1 cut(s) 739
BshFI GGCC 3 cut(s) 122, 612, 1019
BshNI GGYRCC 3 cut(s) 541, 787, 1396
BsiHKAI GWGCWC 2 cut(s) 348, 1534
BsiSI CCGG 1 cut(s) 622
BslFI GGGAC 2 cut(s) 598, 756
BslI CCNNNNNNNGG 4 cut(s) 118, 582, 1015, 1203
BsmAI GTCTC 2 cut(s) 515, 672
BsmFI GGGAC 2 cut(s) 598, 756
BsmI GAATGC 2 cut(s) 617, 1379
BsnI GGCC 3 cut(s) 122, 612, 1019
Bsp1286I GDGCHC 2 cut(s) 348, 1534
Bsp143I GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
BspACI CCGC 4 cut(s) 145, 582, 599, 1628
BspANI GGCC 3 cut(s) 122, 612, 1019
BspCNI CTCAG 4 cut(s) 334, 836, 1057, 1150
BspLI GGNNCC 5 cut(s) 543, 631, 789, 1398, 1632
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 5 cut(s) 146, 445, 815, 953, 1062
BspT107I GGYRCC 3 cut(s) 541, 787, 1396
BsrI ACTGG 1 cut(s) 1307
BssECI CCNNGG 4 cut(s) 1202, 1480, 1516, 1604
BssMI GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
BssT1I CCWWGG 1 cut(s) 1516
Bst2UI CCWGG 3 cut(s) 614, 1481, 1605
Bst4CI ACNGT 6 cut(s) 264, 352, 577, 747, 1077, 1461
Bst6I CTCTTC 3 cut(s) 783, 1112, 1245
BstAPI GCANNNNNTGC 2 cut(s) 1498, 1598
BstC8I GCNNGC 2 cut(s) 973, 1530
BstDEI CTNAG 6 cut(s) 148, 185, 342, 823, 1065, 1158
BstDSI CCRYGG 1 cut(s) 1202
BstEII GGTNACC 1 cut(s) 1345
BstF5I GGATG 4 cut(s) 487, 1195, 1218, 1635
BstKTI GATC 7 cut(s) 154, 440, 639, 810, 961, 1057, 1071
BstMAI GTCTC 2 cut(s) 515, 672
BstMBI GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
BstMWI GCNNNNNNNGC 3 cut(s) 981, 1498, 1598
BstNI CCWGG 3 cut(s) 614, 1481, 1605
BstPI GGTNACC 1 cut(s) 1345
BstSCI CCNGG 3 cut(s) 612, 1479, 1603
BstSFI CTRYAG 2 cut(s) 1470, 1562
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 4 cut(s) 914, 933, 1249, 1566
BstX2I RGATCY 4 cut(s) 151, 437, 958, 1068
BstXI CCANNNNNNTGG 1 cut(s) 552
BstYI RGATCY 4 cut(s) 151, 437, 958, 1068
BsuRI GGCC 3 cut(s) 122, 612, 1019
BtgI CCRYGG 1 cut(s) 1202
BtrI CACGTC 1 cut(s) 1113
BtsCI GGATG 4 cut(s) 487, 1195, 1218, 1635
BtsIMutI CAGTG 1 cut(s) 613
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 2 cut(s) 973, 1530
CaiI CAGNNNCTG 1 cut(s) 356
Cfr13I GGNCC 3 cut(s) 629, 847, 1289
Csp6I GTAC 5 cut(s) 83, 272, 820, 1303, 1462
CspCI CAANNNNNGTGG 2 cut(s) 1505, 1540
CviQI GTAC 5 cut(s) 83, 272, 820, 1303, 1462
DdeI CTNAG 6 cut(s) 148, 185, 342, 823, 1065, 1158
DpnI GATC 7 cut(s) 153, 439, 638, 809, 960, 1056, 1070
DpnII GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
DraI TTTAAA 1 cut(s) 1621
EaeI YGGCCR 3 cut(s) 120, 610, 1017
Eam1104I CTCTTC 3 cut(s) 783, 1112, 1245
EarI CTCTTC 3 cut(s) 783, 1112, 1245
Ecl136II GAGCTC 2 cut(s) 346, 1532
Eco130I CCWWGG 1 cut(s) 1516
Eco24I GRGCYC 2 cut(s) 348, 1534
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 3 cut(s) 629, 847, 1289
Eco53kI GAGCTC 2 cut(s) 346, 1532
Eco57I CTGAAG 1 cut(s) 1269
Eco91I GGTNACC 1 cut(s) 1345
EcoICRI GAGCTC 2 cut(s) 346, 1532
EcoO65I GGTNACC 1 cut(s) 1345
EcoRII CCWGG 3 cut(s) 612, 1479, 1603
EcoRV GATATC 1 cut(s) 298
EcoT14I CCWWGG 1 cut(s) 1516
EcoT38I GRGCYC 2 cut(s) 348, 1534
ErhI CCWWGG 1 cut(s) 1516
FaqI GGGAC 2 cut(s) 598, 756
FauI CCCGC 2 cut(s) 575, 592
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 2 cut(s) 357, 1629
FokI GGATG 4 cut(s) 494, 1202, 1205, 1622
FriOI GRGCYC 2 cut(s) 348, 1534
Fsp4HI GCNGC 2 cut(s) 357, 1629
FspBI CTAG 4 cut(s) 768, 1010, 1139, 1373
GluI GCNGC 2 cut(s) 357, 1629
GsaI CCCAGC 1 cut(s) 743
GsuI CTGGAG 2 cut(s) 677, 955
HaeIII GGCC 3 cut(s) 122, 612, 1019
HapII CCGG 1 cut(s) 622
HincII GTYRAC 1 cut(s) 1312
HindII GTYRAC 1 cut(s) 1312
HindIII AAGCTT 1 cut(s) 314
HinfI GANTC 6 cut(s) 5, 425, 603, 1267, 1452, 1614
HpaII CCGG 1 cut(s) 622
HphI GGTGA 4 cut(s) 162, 506, 560, 565
Hpy166II GTNNAC 4 cut(s) 46, 83, 1132, 1312
Hpy188I TCNGA 8 cut(s) 343, 697, 812, 826, 988, 1059, 1117, 1159
Hpy188III TCNNGA 2 cut(s) 1080, 1545
Hpy8I GTNNAC 4 cut(s) 46, 83, 1132, 1312
HpyAV CCTTC 6 cut(s) 389, 575, 887, 955, 1107, 1223
HpyCH4III ACNGT 6 cut(s) 264, 352, 577, 747, 1077, 1461
HpyCH4IV ACGT 1 cut(s) 1112
HpyCH4V TGCA 6 cut(s) 306, 451, 517, 710, 872, 1379
HpyF10VI GCNNNNNNNGC 3 cut(s) 981, 1498, 1598
HpyF3I CTNAG 6 cut(s) 148, 185, 342, 823, 1065, 1158
HpySE526I ACGT 1 cut(s) 1112
Kzo9I GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
LmnI GCTCC 2 cut(s) 1606, 1636
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 1 cut(s) 762
MaeI CTAG 4 cut(s) 768, 1010, 1139, 1373
MaeII ACGT 1 cut(s) 1112
MaeIII GTNAC 4 cut(s) 197, 371, 664, 1345
MalI GATC 7 cut(s) 153, 439, 638, 809, 960, 1056, 1070
MboI GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
MflI RGATCY 4 cut(s) 151, 437, 958, 1068
MhlI GDGCHC 2 cut(s) 348, 1534
MlsI TGGCCA 3 cut(s) 122, 612, 1019
MluCI AATT 8 cut(s) 113, 684, 771, 874, 906, 963, 977, 1431
MluNI TGGCCA 3 cut(s) 122, 612, 1019
MmeI TCCRAC 2 cut(s) 132, 283
Mox20I TGGCCA 3 cut(s) 122, 612, 1019
MroXI GAANNNNTTC 1 cut(s) 1556
MscI TGGCCA 3 cut(s) 122, 612, 1019
MseI TTAA 5 cut(s) 801, 909, 927, 1436, 1620
MslI CAYNNNNRTG 1 cut(s) 419
Msp20I TGGCCA 3 cut(s) 122, 612, 1019
MspA1I CMGCKG 1 cut(s) 655
MspI CCGG 1 cut(s) 622
MspR9I CCNGG 3 cut(s) 614, 1481, 1605
Mva1269I GAATGC 2 cut(s) 617, 1379
MvaI CCWGG 3 cut(s) 614, 1481, 1605
MwoI GCNNNNNNNGC 3 cut(s) 981, 1498, 1598
NdeII GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
NlaIV GGNNCC 5 cut(s) 543, 631, 789, 1398, 1632
NmuCI GTSAC 2 cut(s) 371, 664
PctI GAATGC 2 cut(s) 617, 1379
PdmI GAANNNNTTC 1 cut(s) 1556
PfeI GAWTC 5 cut(s) 425, 603, 1267, 1452, 1614
PflFI GACNNNGTC 2 cut(s) 369, 589
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 2 cut(s) 358, 1630
PsiI TTATAA 1 cut(s) 905
Psp124BI GAGCTC 2 cut(s) 348, 1534
Psp6I CCWGG 3 cut(s) 612, 1479, 1603
PspEI GGTNACC 1 cut(s) 1345
PspFI CCCAGC 1 cut(s) 739
PspGI CCWGG 3 cut(s) 612, 1479, 1603
PspN4I GGNNCC 5 cut(s) 543, 631, 789, 1398, 1632
PspPI GGNCC 3 cut(s) 629, 847, 1289
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 4 cut(s) 151, 437, 958, 1068
PsyI GACNNNGTC 2 cut(s) 369, 589
PvuII CAGCTG 1 cut(s) 655
RsaI GTAC 5 cut(s) 84, 273, 821, 1304, 1463
RsaNI GTAC 5 cut(s) 83, 272, 820, 1303, 1462
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 2 cut(s) 348, 1534
SaqAI TTAA 5 cut(s) 801, 909, 927, 1436, 1620
SatI GCNGC 2 cut(s) 357, 1629
Sau3AI GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
Sau96I GGNCC 3 cut(s) 629, 847, 1289
ScaI AGTACT 2 cut(s) 273, 1463
ScrFI CCNGG 3 cut(s) 614, 1481, 1605
SduI GDGCHC 2 cut(s) 348, 1534
SfaNI GCATC 1 cut(s) 762
SfcI CTRYAG 2 cut(s) 1470, 1562
SinI GGWCC 3 cut(s) 629, 847, 1289
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 3 cut(s) 202, 393, 431
SmoI CTYRAG 3 cut(s) 202, 393, 431
Sse9I AATT 8 cut(s) 113, 684, 771, 874, 906, 963, 977, 1431
SsiI CCGC 4 cut(s) 145, 582, 599, 1628
SspI AATATT 1 cut(s) 511
SspMI CTAG 4 cut(s) 768, 1010, 1139, 1373
SstI GAGCTC 2 cut(s) 348, 1534
StyD4I CCNGG 3 cut(s) 612, 1479, 1603
StyI CCWWGG 1 cut(s) 1516
TaaI ACNGT 6 cut(s) 264, 352, 577, 747, 1077, 1461
TaiI ACGT 1 cut(s) 1115
TaqI TCGA 2 cut(s) 403, 1546
TasI AATT 8 cut(s) 113, 684, 771, 874, 906, 963, 977, 1431
TatI WGTACW 3 cut(s) 271, 819, 1461
TauI GCSGC 1 cut(s) 1631
TfiI GAWTC 5 cut(s) 425, 603, 1267, 1452, 1614
Tru1I TTAA 5 cut(s) 801, 909, 927, 1436, 1620
Tru9I TTAA 5 cut(s) 801, 909, 927, 1436, 1620
TscAI CASTG 1 cut(s) 613
TseFI GTSAC 2 cut(s) 371, 664
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 2 cut(s) 371, 664
TspDTI ATGAA 5 cut(s) 61, 446, 867, 1139, 1371
TspRI CASTG 1 cut(s) 613
Tth111I GACNNNGTC 2 cut(s) 369, 589
Van91I CCANNNNNTGG 1 cut(s) 118
VpaK11BI GGWCC 3 cut(s) 629, 847, 1289
XapI RAATTY 3 cut(s) 771, 874, 1431
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 45
XmnI GAANNNNTTC 1 cut(s) 1556
XspI CTAG 4 cut(s) 768, 1010, 1139, 1373
ZrmI AGTACT 2 cut(s) 273, 1463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.