RLG00000023246

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012819
Physical Location & Seq
Forward (+)
20484258 .. 20486417
2160 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023246

Sequence Viewer

Length: 1596 bp
ATGGTCTACCTCAAACTTTGCTTGATTTTATTCATGGGTTGTGTACCAGATACAACCAACCTCTGTTCCAACCAATTTCTGGCCACCCCAACCATTATTCCATACCGCTTAGATCCCTCTCATATCACTTCACCTTTTCAAACACTAAGCCTTGATGGTTACTTGAGTTTTGACAACAACCATCATACAGCAAAGGACTTTGGCAACATATACCATTTCCAACCATCAGCAGTACTATATCCAAAATCTGTTTCTGATATCTCCTGCATAATAAAGCTTATATTTGAAATGGGTTCTGGTTCTGAGCTTACAGTTGCTGCCAGAGGACATGGTCACTCCCTTCAAGGTCAAGCTCAAGCTCATCGAGGCATAGTCATCAACATGGAATCACTTACGCAGGGATCTCCAAAAATGCAGGTTCATATTGGAAAGCGACCTTATGTGGATGTTTCGGGTGGTGAGTTATGGATAAATATTCTGCATGAGACACTCAAACATGGGTTGGCACCAAAATCTTGGACAGATTACCTTCACCTCACCGTTGGCGGGACTTTGTCAAATGCCGGAATCAGTGGTCAGGCATTCCGGCATGGACCCCAGATCAGCAATGTCTATCAGCTGGAGGTTGTCACAGGAAAAGGAGACATAATTACCTGTTCCGAAAAGAAAAATGCAGACCTTTTCTATGCTGTTCTGGGAGGGCTGGGACAGTTTGGCATCATTACAAAGGCTAGAATTTCTCTTCAATCGGCACCAAAGATGGTTAAGTGGATCAGAGTGCTGTACTCAGACTTCTCATTATTTTCAAAGGACCAAGAGCTTCTCATATCATCTGCAAATTCATTTGACTACATTGAAGGATTTGTTATAATTAACAGAACAGGTCTTCTTAATAGCTGGAGGTCTTCCTTCAATCCTAAAGATCCAATTCAAGCGAGCCAATTTAGCTCTGATGGTAAAACACTCTACTGCCTAGAAATGGCCAAATACTATAACCCAGAAGAAACTGATAGAACTGAGATCTTACTGTCAGGATTACAATATATCCCATCCACCCTCTTCACGTCAGAAGTTTCCTTCGTAAACTTCCTAGATAGAGTACATTTGTCTGAGAGAAAACTACGAGAAAAAGGATTATGGGATGTTCCCCACCCGTGGATAAACCTTCTCATCCCTAAAAGCAAGATACATGAGTTTGCTGAAGAGGTCTTTGGCAACATTCTCACGGACAATATCAATGGTCCTATCCTCATGTACCCAGTCAACAAAACCAAGTGGAACAACAACACATCTTTGGTTACCCCAGATGAAGATGTGTTCTACCTAGTTGCATTCTTATCCTCTGTGGTGCCATCTTCCACAGGAACAGATGGATTTGACAAAATTTTAACACTAAACAGAAGAATCTTACAGTACTCTGGCACAGCTAACCTGGGTATCAAGCAATATCTGCCCCATTACAGAACCCAAGGAGAGTGGCGAGCTCATTTTGGCTCTCGATGGAAAGTTTTCCTACAGAGGAAGTCTTTTTATGACCCTTTGGCAATCCTTGCTCCTGGGCAGAGAATCTTTAAAAAGGCGGCTCCCATCCTATGA

Protein Analysis

532

Amino Acids

59.6

Weight (kDa)

8.65

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 75 - 220 4.8e-17 FAD binding domain
Cytokin-bind PF09265 252 - 524 1.4e-110 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41510 AT2G41510 AT2G41510
fragaria_vesca FvH4_6g24620
malus_domestica MD12G1083600.v1.1 MD14G1078600.v1.1
prunus_persica Prupe.7G052300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0483951
rosa_laevigata RLG00000023246
rosa_multiflora Rmu_sc0003477.1_g000016
rosa_roxburghii Rroxscaffold_6G00397930
rosa_rugosa Rorug03G0209600 Rorug03G0209700
rosa_samantha Rh3AG257900 Rh3BG295000 Rh3CG292400 Rh3DG288000
rosa_wichuraiana Rw3G023300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 869
Acc36I ACCTGC 1 cut(s) 406
AccB1I GGYRCC 3 cut(s) 505, 751, 1348
AccB7I CCANNNNNTGG 1 cut(s) 79
AccI GTMKAC 1 cut(s) 6
AciI CCGC 3 cut(s) 106, 546, 1580
AclWI GGATC 4 cut(s) 107, 409, 779, 917
AcoI YGGCCR 2 cut(s) 81, 981
AcsI RAATTY 3 cut(s) 735, 838, 1383
AcuI CTGAAG 1 cut(s) 1221
AfaI GTAC 6 cut(s) 45, 234, 785, 1101, 1256, 1415
AfiI CCNNNNNNNGG 4 cut(s) 79, 546, 979, 1155
AgsI TTSAA 8 cut(s) 140, 287, 344, 746, 807, 857, 913, 932
AjiI CACGTC 1 cut(s) 1065
AjnI CCWGG 2 cut(s) 1431, 1555
AjuI GAANNNNNNNTTGG 4 cut(s) 235, 267, 1194, 1226
Alw21I GWGCWC 1 cut(s) 1486
Alw26I GTCTC 2 cut(s) 479, 636
AlwI GGATC 4 cut(s) 107, 409, 779, 917
AlwNI CAGNNNCTG 1 cut(s) 317
AoxI GGCC 2 cut(s) 81, 981
ApeKI GCWGC 1 cut(s) 317
ApoI RAATTY 3 cut(s) 735, 838, 1383
Asp700I GAANNNNTTC 1 cut(s) 1508
AspS9I GGNCC 3 cut(s) 593, 811, 1241
AsuHPI GGTGA 4 cut(s) 123, 470, 524, 529
AvaII GGWCC 3 cut(s) 593, 811, 1241
BaeI ACNNNNGTAYC 4 cut(s) 1178, 1211, 1420, 1453
BalI TGGCCA 2 cut(s) 83, 983
BanI GGYRCC 3 cut(s) 505, 751, 1348
BanII GRGCYC 1 cut(s) 1486
BarI GAAGNNNNNNTAC 2 cut(s) 776, 808
BbsI GAAGAC 2 cut(s) 878, 897
Bbv12I GWGCWC 1 cut(s) 1486
BbvI GCAGC 1 cut(s) 304
BciT130I CCWGG 2 cut(s) 1433, 1557
BcoDI GTCTC 2 cut(s) 479, 636
BfaI CTAG 4 cut(s) 732, 974, 1091, 1325
BfmI CTRYAG 1 cut(s) 1514
BfuAI ACCTGC 1 cut(s) 406
BglII AGATCT 1 cut(s) 1020
BisI GCNGC 2 cut(s) 318, 1581
BlsI GCNGC 2 cut(s) 319, 1582
BmcAI AGTACT 2 cut(s) 234, 1415
Bme1390I CCNGG 2 cut(s) 1433, 1557
Bme18I GGWCC 3 cut(s) 593, 811, 1241
BmgBI CACGTC 1 cut(s) 1065
BmgT120I GGNCC 3 cut(s) 593, 811, 1241
BmiI GGNNCC 5 cut(s) 507, 595, 753, 1350, 1584
BmrFI CCNGG 2 cut(s) 1433, 1557
BmrI ACTGGG 1 cut(s) 1253
BmsI GCATC 1 cut(s) 726
BmuI ACTGGG 1 cut(s) 1253
BpiI GAAGAC 2 cut(s) 878, 897
BpmI CTGGAG 2 cut(s) 641, 919
BpuEI CTTGAG 2 cut(s) 184, 339
BsaJI CCNNGG 4 cut(s) 1154, 1432, 1468, 1556
Bsc4I CCNNNNNNNGG 4 cut(s) 79, 546, 979, 1155
Bse1I ACTGG 1 cut(s) 1259
Bse3DI GCAATG 1 cut(s) 613
BseBI CCWGG 2 cut(s) 1433, 1557
BseDI CCNNGG 4 cut(s) 1154, 1432, 1468, 1556
BseGI GGATG 5 cut(s) 451, 1049, 1147, 1170, 1587
BseLI CCNNNNNNNGG 4 cut(s) 79, 546, 979, 1155
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 4 cut(s) 294, 801, 1008, 1101
BseNI ACTGG 1 cut(s) 1259
BseXI GCAGC 1 cut(s) 304
BseYI CCCAGC 1 cut(s) 703
BshFI GGCC 2 cut(s) 83, 983
BshNI GGYRCC 3 cut(s) 505, 751, 1348
BsiHKAI GWGCWC 1 cut(s) 1486
BsiSI CCGG 2 cut(s) 564, 586
BslFI GGGAC 2 cut(s) 562, 720
BslI CCNNNNNNNGG 4 cut(s) 79, 546, 979, 1155
BsmAI GTCTC 2 cut(s) 479, 636
BsmFI GGGAC 2 cut(s) 562, 720
BsmI GAATGC 2 cut(s) 581, 1331
BsnI GGCC 2 cut(s) 83, 983
Bsp1286I GDGCHC 1 cut(s) 1486
Bsp143I GATC 6 cut(s) 112, 401, 600, 771, 922, 1020
BspACI CCGC 3 cut(s) 106, 546, 1580
BspANI GGCC 2 cut(s) 83, 983
BspCNI CTCAG 4 cut(s) 295, 800, 1009, 1102
BspLI GGNNCC 5 cut(s) 507, 595, 753, 1350, 1584
BspMI ACCTGC 1 cut(s) 406
BspPI GGATC 4 cut(s) 107, 409, 779, 917
BspT107I GGYRCC 3 cut(s) 505, 751, 1348
BsrDI GCAATG 1 cut(s) 613
BsrI ACTGG 1 cut(s) 1259
BssECI CCNNGG 4 cut(s) 1154, 1432, 1468, 1556
BssMI GATC 6 cut(s) 112, 401, 600, 771, 922, 1020
BssT1I CCWWGG 1 cut(s) 1468
Bst2UI CCWGG 2 cut(s) 1433, 1557
Bst4CI ACNGT 5 cut(s) 313, 541, 711, 1029, 1413
Bst6I CTCTTC 3 cut(s) 747, 1064, 1197
BstAPI GCANNNNNTGC 2 cut(s) 1450, 1550
BstC8I GCNNGC 2 cut(s) 937, 1482
BstDEI CTNAG 6 cut(s) 109, 146, 303, 787, 1017, 1110
BstDSI CCRYGG 1 cut(s) 1154
BstEII GGTNACC 1 cut(s) 1297
BstF5I GGATG 5 cut(s) 451, 1049, 1147, 1170, 1587
BstKTI GATC 6 cut(s) 115, 404, 603, 774, 925, 1023
BstMAI GTCTC 2 cut(s) 479, 636
BstMBI GATC 6 cut(s) 112, 401, 600, 771, 922, 1020
BstMWI GCNNNNNNNGC 3 cut(s) 945, 1450, 1550
BstNI CCWGG 2 cut(s) 1433, 1557
BstPI GGTNACC 1 cut(s) 1297
BstSCI CCNGG 2 cut(s) 1431, 1555
BstSFI CTRYAG 1 cut(s) 1514
BstV1I GCAGC 1 cut(s) 304
BstV2I GAAGAC 2 cut(s) 878, 897
BstX2I RGATCY 4 cut(s) 112, 401, 922, 1020
BstXI CCANNNNNNTGG 1 cut(s) 516
BstYI RGATCY 4 cut(s) 112, 401, 922, 1020
BsuRI GGCC 2 cut(s) 83, 983
BtgI CCRYGG 1 cut(s) 1154
BtrI CACGTC 1 cut(s) 1065
BtsCI GGATG 5 cut(s) 451, 1049, 1147, 1170, 1587
BtsIMutI CAGTG 1 cut(s) 577
BveI ACCTGC 1 cut(s) 406
Cac8I GCNNGC 2 cut(s) 937, 1482
CaiI CAGNNNCTG 1 cut(s) 317
Cfr13I GGNCC 3 cut(s) 593, 811, 1241
Csp6I GTAC 6 cut(s) 44, 233, 784, 1100, 1255, 1414
CspCI CAANNNNNGTGG 2 cut(s) 1457, 1492
CviAII CATG 8 cut(s) 34, 329, 382, 482, 497, 590, 1190, 1252
CviQI GTAC 6 cut(s) 44, 233, 784, 1100, 1255, 1414
DdeI CTNAG 6 cut(s) 109, 146, 303, 787, 1017, 1110
DpnI GATC 6 cut(s) 114, 403, 602, 773, 924, 1022
DpnII GATC 6 cut(s) 112, 401, 600, 771, 922, 1020
DraI TTTAAA 1 cut(s) 1573
EaeI YGGCCR 2 cut(s) 81, 981
Eam1104I CTCTTC 3 cut(s) 747, 1064, 1197
EarI CTCTTC 3 cut(s) 747, 1064, 1197
Ecl136II GAGCTC 1 cut(s) 1484
Eco130I CCWWGG 1 cut(s) 1468
Eco24I GRGCYC 1 cut(s) 1486
Eco32I GATATC 1 cut(s) 259
Eco47I GGWCC 3 cut(s) 593, 811, 1241
Eco53kI GAGCTC 1 cut(s) 1484
Eco57I CTGAAG 1 cut(s) 1221
Eco91I GGTNACC 1 cut(s) 1297
EcoICRI GAGCTC 1 cut(s) 1484
EcoO65I GGTNACC 1 cut(s) 1297
EcoRII CCWGG 2 cut(s) 1431, 1555
EcoRV GATATC 1 cut(s) 259
EcoT14I CCWWGG 1 cut(s) 1468
EcoT38I GRGCYC 1 cut(s) 1486
ErhI CCWWGG 1 cut(s) 1468
FaeI CATG 8 cut(s) 37, 332, 385, 485, 500, 593, 1193, 1255
FaqI GGGAC 2 cut(s) 562, 720
FatI CATG 8 cut(s) 33, 328, 381, 481, 496, 589, 1189, 1251
FauI CCCGC 1 cut(s) 539
FblI GTMKAC 1 cut(s) 6
Fnu4HI GCNGC 2 cut(s) 318, 1581
FokI GGATG 5 cut(s) 458, 1036, 1154, 1157, 1574
FriOI GRGCYC 1 cut(s) 1486
Fsp4HI GCNGC 2 cut(s) 318, 1581
FspBI CTAG 4 cut(s) 732, 974, 1091, 1325
GluI GCNGC 2 cut(s) 318, 1581
GsaI CCCAGC 1 cut(s) 707
GsuI CTGGAG 2 cut(s) 641, 919
HaeIII GGCC 2 cut(s) 83, 983
HapII CCGG 2 cut(s) 564, 586
Hin1II CATG 8 cut(s) 37, 332, 385, 485, 500, 593, 1193, 1255
HincII GTYRAC 1 cut(s) 1264
HindII GTYRAC 1 cut(s) 1264
HindIII AAGCTT 1 cut(s) 275
HinfI GANTC 4 cut(s) 386, 567, 1404, 1566
HpaII CCGG 2 cut(s) 564, 586
HphI GGTGA 4 cut(s) 123, 470, 524, 529
Hpy166II GTNNAC 4 cut(s) 7, 44, 1084, 1264
Hpy188I TCNGA 8 cut(s) 256, 304, 661, 776, 790, 952, 1069, 1111
Hpy188III TCNNGA 2 cut(s) 1032, 1497
Hpy8I GTNNAC 4 cut(s) 7, 44, 1084, 1264
HpyAV CCTTC 6 cut(s) 350, 539, 851, 919, 1087, 1175
HpyCH4III ACNGT 5 cut(s) 313, 541, 711, 1029, 1413
HpyCH4IV ACGT 1 cut(s) 1064
HpyCH4V TGCA 6 cut(s) 267, 415, 481, 674, 836, 1331
HpyF10VI GCNNNNNNNGC 3 cut(s) 945, 1450, 1550
HpyF3I CTNAG 6 cut(s) 109, 146, 303, 787, 1017, 1110
HpySE526I ACGT 1 cut(s) 1064
Hsp92II CATG 8 cut(s) 37, 332, 385, 485, 500, 593, 1193, 1255
Kzo9I GATC 6 cut(s) 112, 401, 600, 771, 922, 1020
LmnI GCTCC 2 cut(s) 1558, 1588
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 1 cut(s) 726
MaeI CTAG 4 cut(s) 732, 974, 1091, 1325
MaeII ACGT 1 cut(s) 1064
MaeIII GTNAC 4 cut(s) 158, 332, 628, 1297
MalI GATC 6 cut(s) 114, 403, 602, 773, 924, 1022
MboI GATC 6 cut(s) 112, 401, 600, 771, 922, 1020
MboII GAAGA 9 cut(s) 734, 878, 897, 1013, 1051, 1214, 1322, 1347, 1413
MflI RGATCY 4 cut(s) 112, 401, 922, 1020
MhlI GDGCHC 1 cut(s) 1486
MlsI TGGCCA 2 cut(s) 83, 983
MluCI AATT 8 cut(s) 74, 648, 735, 838, 870, 927, 941, 1383
MluNI TGGCCA 2 cut(s) 83, 983
MmeI TCCRAC 2 cut(s) 93, 244
Mox20I TGGCCA 2 cut(s) 83, 983
MroXI GAANNNNTTC 1 cut(s) 1508
MscI TGGCCA 2 cut(s) 83, 983
MseI TTAA 5 cut(s) 765, 873, 891, 1388, 1572
MslI CAYNNNNRTG 1 cut(s) 380
Msp20I TGGCCA 2 cut(s) 83, 983
MspA1I CMGCKG 1 cut(s) 619
MspI CCGG 2 cut(s) 564, 586
MspR9I CCNGG 2 cut(s) 1433, 1557
Mva1269I GAATGC 2 cut(s) 581, 1331
MvaI CCWGG 2 cut(s) 1433, 1557
MwoI GCNNNNNNNGC 3 cut(s) 945, 1450, 1550
NdeII GATC 6 cut(s) 112, 401, 600, 771, 922, 1020
NlaIII CATG 8 cut(s) 37, 332, 385, 485, 500, 593, 1193, 1255
NlaIV GGNNCC 5 cut(s) 507, 595, 753, 1350, 1584
NmuCI GTSAC 2 cut(s) 332, 628
PctI GAATGC 2 cut(s) 581, 1331
PdmI GAANNNNTTC 1 cut(s) 1508
PfeI GAWTC 4 cut(s) 386, 567, 1404, 1566
PflFI GACNNNGTC 2 cut(s) 330, 553
PflMI CCANNNNNTGG 1 cut(s) 79
PkrI GCNGC 2 cut(s) 319, 1582
PsiI TTATAA 1 cut(s) 869
Psp124BI GAGCTC 1 cut(s) 1486
Psp6I CCWGG 2 cut(s) 1431, 1555
PspEI GGTNACC 1 cut(s) 1297
PspFI CCCAGC 1 cut(s) 703
PspGI CCWGG 2 cut(s) 1431, 1555
PspN4I GGNNCC 5 cut(s) 507, 595, 753, 1350, 1584
PspPI GGNCC 3 cut(s) 593, 811, 1241
PstNI CAGNNNCTG 1 cut(s) 317
PsuI RGATCY 4 cut(s) 112, 401, 922, 1020
PsyI GACNNNGTC 2 cut(s) 330, 553
PvuII CAGCTG 1 cut(s) 619
RsaI GTAC 6 cut(s) 45, 234, 785, 1101, 1256, 1415
RsaNI GTAC 6 cut(s) 44, 233, 784, 1100, 1255, 1414
RseI CAYNNNNRTG 1 cut(s) 380
SacI GAGCTC 1 cut(s) 1486
SaqAI TTAA 5 cut(s) 765, 873, 891, 1388, 1572
SatI GCNGC 2 cut(s) 318, 1581
Sau3AI GATC 6 cut(s) 112, 401, 600, 771, 922, 1020
Sau96I GGNCC 3 cut(s) 593, 811, 1241
ScaI AGTACT 2 cut(s) 234, 1415
ScrFI CCNGG 2 cut(s) 1433, 1557
SduI GDGCHC 1 cut(s) 1486
SfaNI GCATC 1 cut(s) 726
SfcI CTRYAG 1 cut(s) 1514
SinI GGWCC 3 cut(s) 593, 811, 1241
SmiMI CAYNNNNRTG 1 cut(s) 380
SmlI CTYRAG 2 cut(s) 163, 354
SmoI CTYRAG 2 cut(s) 163, 354
Sse9I AATT 8 cut(s) 74, 648, 735, 838, 870, 927, 941, 1383
SsiI CCGC 3 cut(s) 106, 546, 1580
SspI AATATT 1 cut(s) 475
SspMI CTAG 4 cut(s) 732, 974, 1091, 1325
SstI GAGCTC 1 cut(s) 1486
StyD4I CCNGG 2 cut(s) 1431, 1555
StyI CCWWGG 1 cut(s) 1468
TaaI ACNGT 5 cut(s) 313, 541, 711, 1029, 1413
TaiI ACGT 1 cut(s) 1067
TaqI TCGA 2 cut(s) 364, 1498
TasI AATT 8 cut(s) 74, 648, 735, 838, 870, 927, 941, 1383
TatI WGTACW 4 cut(s) 232, 783, 1099, 1413
TauI GCSGC 1 cut(s) 1583
TfiI GAWTC 4 cut(s) 386, 567, 1404, 1566
Tru1I TTAA 5 cut(s) 765, 873, 891, 1388, 1572
Tru9I TTAA 5 cut(s) 765, 873, 891, 1388, 1572
TscAI CASTG 1 cut(s) 577
TseFI GTSAC 2 cut(s) 332, 628
TseI GCWGC 1 cut(s) 317
Tsp45I GTSAC 2 cut(s) 332, 628
TspDTI ATGAA 4 cut(s) 22, 410, 831, 1323
TspGWI ACGGA 1 cut(s) 1241
TspRI CASTG 1 cut(s) 577
Tth111I GACNNNGTC 2 cut(s) 330, 553
Van91I CCANNNNNTGG 1 cut(s) 79
VpaK11BI GGWCC 3 cut(s) 593, 811, 1241
XapI RAATTY 3 cut(s) 735, 838, 1383
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 6
XmnI GAANNNNTTC 1 cut(s) 1508
XspI CTAG 4 cut(s) 732, 974, 1091, 1325
ZrmI AGTACT 2 cut(s) 234, 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.