Rorug03G0209700

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0012819
Physical Location & Seq
Forward (+)
18694526 .. 18695534
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0209700.1

Sequence Viewer

Length: 342 bp
ATGAGTCGCAGAAGCGGAGGCGGTCCGAAGCTTGATCTGAAGCTTAACCTTTCCCCACCGAGGGCAAACCCTACGGCTGAGTCTCCCGGACGATCATCCACCGTGTCCCCCACGTCCCCGCCGAGTTCATGCGTGTCGTCGGAGCTGACTCAGGACGACAACAACAACATGAGGTACTCCAACAGCCCCGAGGTCACTTCCATGGTGCTCGTGGGTTGCCCCAGGTGTCTCATGTACGTCATGCTATCCGAGGACGACCCGAAATGCCCCAAATGCAAGAGCACTGTTCTGCTGGACTTCCTCCATGAGAACAGCAACACCATGACGACAAGGAAGAGCTAA

Protein Analysis

113

Amino Acids

12.27

Weight (kDa)

8.33

Isoelectric Point (pI)

70.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn-ribbon_GIR1 PF24747 66 - 102 5.6e-29 GIR1-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41510 AT2G41510 AT2G41510
fragaria_vesca FvH4_6g24620
malus_domestica MD12G1083600.v1.1 MD14G1078600.v1.1
prunus_persica Prupe.7G052300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0483951
rosa_laevigata RLG00000023246
rosa_multiflora Rmu_sc0003477.1_g000016
rosa_roxburghii Rroxscaffold_6G00397930
rosa_rugosa Rorug03G0209600 Rorug03G0209700
rosa_samantha Rh3AG257900 Rh3BG295000 Rh3CG292400 Rh3DG288000
rosa_wichuraiana Rw3G023300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 15, 21, 119
AcuI CTGAAG 1 cut(s) 59
AfaI GTAC 2 cut(s) 176, 236
AfiI CCNNNNNNNGG 2 cut(s) 60, 61
AjiI CACGTC 1 cut(s) 114
AjnI CCWGG 1 cut(s) 221
AluBI AGCT 4 cut(s) 31, 43, 145, 339
AluI AGCT 4 cut(s) 31, 43, 145, 339
Alw21I GWGCWC 2 cut(s) 210, 284
Alw26I GTCTC 2 cut(s) 87, 233
Ama87I CYCGRG 1 cut(s) 188
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 87
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 1 cut(s) 23
BauI CACGAG 1 cut(s) 209
Bbv12I GWGCWC 2 cut(s) 210, 284
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 1 cut(s) 223
BcnI CCSGG 1 cut(s) 87
BcoDI GTCTC 2 cut(s) 87, 233
Bme1390I CCNGG 2 cut(s) 87, 223
Bme18I GGWCC 1 cut(s) 23
BmeT110I CYCGRG 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 23
BmrFI CCNGG 2 cut(s) 87, 223
BpuMI CCSGG 1 cut(s) 87
BsaJI CCNNGG 5 cut(s) 59, 189, 201, 221, 249
Bsc4I CCNNNNNNNGG 2 cut(s) 60, 61
BseBI CCWGG 1 cut(s) 223
BseDI CCNNGG 5 cut(s) 59, 189, 201, 221, 249
BseGI GGATG 1 cut(s) 95
BseLI CCNNNNNNNGG 2 cut(s) 60, 61
BseMII CTCAG 2 cut(s) 69, 164
BsiHKAI GWGCWC 2 cut(s) 210, 284
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 2 cut(s) 91, 100
BslI CCNNNNNNNGG 2 cut(s) 60, 61
BsmAI GTCTC 2 cut(s) 87, 233
BsmFI GGGAC 2 cut(s) 91, 100
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 210, 284
Bsp143I GATC 2 cut(s) 34, 92
Bsp19I CCATGG 1 cut(s) 201
BspACI CCGC 3 cut(s) 15, 21, 119
BspCNI CTCAG 2 cut(s) 70, 163
BspQI GCTCTTC 1 cut(s) 329
BssECI CCNNGG 5 cut(s) 59, 189, 201, 221, 249
BssMI GATC 2 cut(s) 34, 92
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 201
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 1 cut(s) 223
Bst4CI ACNGT 2 cut(s) 103, 286
Bst6I CTCTTC 1 cut(s) 329
BstDEI CTNAG 2 cut(s) 78, 150
BstDSI CCRYGG 1 cut(s) 201
BstF5I GGATG 1 cut(s) 95
BstKTI GATC 2 cut(s) 37, 95
BstMAI GTCTC 2 cut(s) 87, 233
BstMBI GATC 2 cut(s) 34, 92
BstMWI GCNNNNNNNGC 1 cut(s) 273
BstNI CCWGG 1 cut(s) 223
BstSCI CCNGG 2 cut(s) 85, 221
BtgI CCRYGG 1 cut(s) 201
BtrI CACGTC 1 cut(s) 114
BtsCI GGATG 1 cut(s) 95
BtsIMutI CAGTG 1 cut(s) 282
Cfr13I GGNCC 1 cut(s) 23
CpoI CGGWCCG 1 cut(s) 23
Csp6I GTAC 2 cut(s) 175, 235
CspI CGGWCCG 1 cut(s) 23
CviAII CATG 7 cut(s) 129, 169, 202, 232, 241, 305, 322
CviJI RGCY 6 cut(s) 31, 43, 77, 145, 186, 339
CviKI_1 RGCY 6 cut(s) 31, 43, 77, 145, 186, 339
CviQI GTAC 2 cut(s) 175, 235
DdeI CTNAG 2 cut(s) 78, 150
DpnI GATC 2 cut(s) 36, 94
DpnII GATC 2 cut(s) 34, 92
Eam1104I CTCTTC 1 cut(s) 329
EarI CTCTTC 1 cut(s) 329
Eco130I CCWWGG 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 23
Eco57I CTGAAG 1 cut(s) 59
Eco88I CYCGRG 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 221
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
FaeI CATG 7 cut(s) 132, 172, 205, 235, 244, 308, 325
FaiI YATR 7 cut(s) 130, 170, 203, 233, 242, 306, 323
FaqI GGGAC 2 cut(s) 91, 100
FatI CATG 7 cut(s) 128, 168, 201, 231, 240, 304, 321
FauI CCCGC 1 cut(s) 126
FokI GGATG 1 cut(s) 82
HapII CCGG 1 cut(s) 87
Hin1II CATG 7 cut(s) 132, 172, 205, 235, 244, 308, 325
HindIII AAGCTT 2 cut(s) 29, 41
HinfI GANTC 3 cut(s) 4, 80, 148
HpaII CCGG 1 cut(s) 87
Hpy188I TCNGA 4 cut(s) 27, 39, 142, 250
Hpy188III TCNNGA 1 cut(s) 152
Hpy99I CGWCG 1 cut(s) 142
HpyCH4III ACNGT 2 cut(s) 103, 286
HpyCH4IV ACGT 2 cut(s) 113, 237
HpyCH4V TGCA 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 1 cut(s) 273
HpyF3I CTNAG 2 cut(s) 78, 150
HpySE526I ACGT 2 cut(s) 113, 237
Hsp92II CATG 7 cut(s) 132, 172, 205, 235, 244, 308, 325
Kzo9I GATC 2 cut(s) 34, 92
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 1 cut(s) 142
LpnPI CCDG 5 cut(s) 100, 137, 208, 235, 278
MaeII ACGT 2 cut(s) 113, 237
MaeIII GTNAC 1 cut(s) 193
MalI GATC 2 cut(s) 36, 94
MboI GATC 2 cut(s) 34, 92
MhlI GDGCHC 2 cut(s) 210, 284
MlyI GAGTC 3 cut(s) 13, 89, 142
MmeI TCCRAC 2 cut(s) 120, 204
MnlI CCTC 6 cut(s) 11, 54, 165, 184, 244, 311
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 1 cut(s) 200
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 2 cut(s) 87, 223
MvaI CCWGG 1 cut(s) 223
MwoI GCNNNNNNNGC 1 cut(s) 273
NciI CCSGG 1 cut(s) 87
NcoI CCATGG 1 cut(s) 201
NdeII GATC 2 cut(s) 34, 92
NlaIII CATG 7 cut(s) 132, 172, 205, 235, 244, 308, 325
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 1 cut(s) 193
PciSI GCTCTTC 1 cut(s) 329
PcsI WCGNNNNNNNCGW 1 cut(s) 119
PfoI TCCNGGA 1 cut(s) 85
PleI GAGTC 3 cut(s) 12, 88, 142
PpsI GAGTC 3 cut(s) 12, 88, 142
Psp6I CCWGG 1 cut(s) 221
PspGI CCWGG 1 cut(s) 221
PspPI GGNCC 1 cut(s) 23
RsaI GTAC 2 cut(s) 176, 236
RsaNI GTAC 2 cut(s) 175, 235
RseI CAYNNNNRTG 1 cut(s) 200
Rsr2I CGGWCCG 1 cut(s) 23
RsrII CGGWCCG 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 45
Sau3AI GATC 2 cut(s) 34, 92
Sau96I GGNCC 1 cut(s) 23
SchI GAGTC 3 cut(s) 13, 89, 142
ScrFI CCNGG 2 cut(s) 87, 223
SduI GDGCHC 2 cut(s) 210, 284
SinI GGWCC 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 200
SsiI CCGC 3 cut(s) 15, 21, 119
StyD4I CCNGG 2 cut(s) 85, 221
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 2 cut(s) 103, 286
TaiI ACGT 2 cut(s) 116, 240
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 289
TseFI GTSAC 1 cut(s) 193
Tsp45I GTSAC 1 cut(s) 193
TspDTI ATGAA 1 cut(s) 117
TspRI CASTG 1 cut(s) 289
VpaK11BI GGWCC 1 cut(s) 23
XcmI CCANNNNNNNNNTGG 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.