Rh3DG288000

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0012819
Physical Location & Seq
Reverse (-)
29709973 .. 29715066
5094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG288000.1

Sequence Viewer

Length: 1356 bp
ATGGACTCACCTCCATACAGCTTCCACAAACAGAACAACATGGTCTACCTCAAACTTTGCTTGATTTTATTCATGGGTTGTGTACCAGATACAACCAACATCTGTTCCAACCAATTTCTGGCCACCCCAACCATTATTCCATACCGCTTAGATCCATCTCATATCACTTCACCTTTTCAAACACTAAGCCTTGATGGTTACTTGAGTTTTGACAACAACCATCACACAGCAAAGGACTTTGGCAACATATACCATTTCCAACCGTCAGCAGTACTATATCCAAAATCTGTTTCTGATATCTCCTGCATAATAAAGCATATATTTGAAATGGGTTCTGGTTCTGAGCTCACAGTTGCTGCCAGAGGACATGGTCACTCCCTTCAAGGTCAAGCTCAAGCTCATCGAGGCATAGTCATCAGCATGGAATCACTTAAGGGATCTCCAAAAATGCAGGTTCATATTGGAAAGCGACCTTATGTGGATGTTTCGGGTGGTGAGTTATGGATAAATATTCTGCATGAGACACTCAAACATGGGTTGGCACCAAAATCTTGGACAGATTACCTTCACCTCACCGTTGGCGGGACTTTGTCAAATGCCGGAATCAGTGGTCAGGCATTCCGGCATGGACCCCAGATCAACAATGTCTATCAGCTGGAGGTTGTCACAGGAAAAGGAGACATAATTACCTGTTCCGAAAAGAAAAATGCAGACCTTTTCTATGCTGTTCTGGGAGGGCTGGGACAGTTTGGCATCATTACAAAGGCTAGAATTTCTCTTCAATCGGCACCAAAGATGGTTAAGTGGATCAGAGTGCTGTACTCAGACTTCTCATTATTTTCAAAGGACCAAGAGCTTCTCATATCATCTGCAAATTCATTTGACTACATTGAAGGATTTGTTATAATTAACAGAACAGGTCTTCTTAATAGCTGGAGGTCTTCCTTCAATCCTAAAGATCCAATTCACGCCAGCCAATTTAGCTCGGATGGTAAAACACTCTACTGCCTAGAAATGGCCAAATACTATAACCCAGACGAAACTGATGTCATGGATCAGAGAACTGAGATCTTACTGTCAGGATTACAATATATCCCATCCACCCTCTTCACGTCAGAAGTTTCCTTTGTAAACTTCCTAGATAGAGTTCATTTGTCTGAGAGAAAACTACGAGAAAAAGGATTATGGGATGTTCCCCACCCGTGGATAAACCTTCTCATCCCTAAAAGCAAGATACATGAGTTTGCTGAAGAGGTCTTTGGCAACATTCTCACAGACAATATCAATGGTCCTATCCTCATGTACCCAGTCAACAAAACCAAGTACCTCTTCTACAGTTTTATGACTTCTTTTTAA

Protein Analysis

451

Amino Acids

50.72

Weight (kDa)

7.01

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 88 - 232 8.5e-18 FAD binding domain
Cytokin-bind PF09265 264 - 442 2.9e-70 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41510 AT2G41510 AT2G41510
fragaria_vesca FvH4_6g24620
malus_domestica MD12G1083600.v1.1 MD14G1078600.v1.1
prunus_persica Prupe.7G052300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0483951
rosa_laevigata RLG00000023246
rosa_multiflora Rmu_sc0003477.1_g000016
rosa_roxburghii Rroxscaffold_6G00397930
rosa_rugosa Rorug03G0209600 Rorug03G0209700
rosa_samantha Rh3AG257900 Rh3BG295000 Rh3CG292400 Rh3DG288000
rosa_wichuraiana Rw3G023300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
Acc36I ACCTGC 1 cut(s) 442
AccB1I GGYRCC 2 cut(s) 541, 787
AccB7I CCANNNNNTGG 1 cut(s) 118
AccI GTMKAC 1 cut(s) 45
AciI CCGC 2 cut(s) 145, 582
AclWI GGATC 5 cut(s) 146, 445, 815, 953, 1062
AcoI YGGCCR 2 cut(s) 120, 1017
AcsI RAATTY 2 cut(s) 771, 874
AcuI CTGAAG 1 cut(s) 1269
AfaI GTAC 5 cut(s) 84, 273, 821, 1304, 1325
AfiI CCNNNNNNNGG 4 cut(s) 118, 582, 1015, 1203
AflII CTTAAG 1 cut(s) 431
AgsI TTSAA 7 cut(s) 179, 326, 383, 782, 843, 893, 949
AjiI CACGTC 1 cut(s) 1113
AjuI GAANNNNNNNTTGG 6 cut(s) 274, 306, 1242, 1274, 1313, 1345
AluBI AGCT 8 cut(s) 21, 346, 392, 398, 655, 856, 933, 984
AluI AGCT 8 cut(s) 21, 346, 392, 398, 655, 856, 933, 984
Alw21I GWGCWC 1 cut(s) 348
Alw26I GTCTC 2 cut(s) 515, 672
AlwI GGATC 5 cut(s) 146, 445, 815, 953, 1062
AlwNI CAGNNNCTG 1 cut(s) 356
AoxI GGCC 2 cut(s) 120, 1017
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 2 cut(s) 771, 874
ArsI GACNNNNNNTTYG 2 cut(s) 1032, 1064
AspS9I GGNCC 3 cut(s) 629, 847, 1289
AsuHPI GGTGA 4 cut(s) 162, 506, 560, 565
AvaII GGWCC 3 cut(s) 629, 847, 1289
BaeI ACNNNNGTAYC 2 cut(s) 1226, 1259
BalI TGGCCA 2 cut(s) 122, 1019
BanI GGYRCC 2 cut(s) 541, 787
BanII GRGCYC 1 cut(s) 348
BarI GAAGNNNNNNTAC 2 cut(s) 812, 844
BbsI GAAGAC 2 cut(s) 914, 933
Bbv12I GWGCWC 1 cut(s) 348
BbvI GCAGC 1 cut(s) 343
BccI CCATC 6 cut(s) 163, 188, 228, 790, 983, 1105
BcoDI GTCTC 2 cut(s) 515, 672
BfaI CTAG 3 cut(s) 768, 1010, 1139
BfmI CTRYAG 1 cut(s) 1333
BfrI CTTAAG 1 cut(s) 431
BfuAI ACCTGC 1 cut(s) 442
BglII AGATCT 1 cut(s) 1068
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
BmcAI AGTACT 1 cut(s) 273
Bme18I GGWCC 3 cut(s) 629, 847, 1289
BmgBI CACGTC 1 cut(s) 1113
BmgT120I GGNCC 3 cut(s) 629, 847, 1289
BmiI GGNNCC 3 cut(s) 543, 631, 789
BmrI ACTGGG 1 cut(s) 1301
BmsI GCATC 1 cut(s) 762
BmuI ACTGGG 1 cut(s) 1301
BpiI GAAGAC 2 cut(s) 914, 933
BpmI CTGGAG 2 cut(s) 677, 955
BpuEI CTTGAG 2 cut(s) 223, 378
BsaJI CCNNGG 1 cut(s) 1202
Bsc4I CCNNNNNNNGG 4 cut(s) 118, 582, 1015, 1203
Bse1I ACTGG 1 cut(s) 1307
BseDI CCNNGG 1 cut(s) 1202
BseGI GGATG 5 cut(s) 487, 994, 1097, 1195, 1218
BseLI CCNNNNNNNGG 4 cut(s) 118, 582, 1015, 1203
BseMII CTCAG 4 cut(s) 333, 837, 1056, 1149
BseNI ACTGG 1 cut(s) 1307
BseXI GCAGC 1 cut(s) 343
BseYI CCCAGC 1 cut(s) 739
BshFI GGCC 2 cut(s) 122, 1019
BshNI GGYRCC 2 cut(s) 541, 787
BsiHKAI GWGCWC 1 cut(s) 348
BsiSI CCGG 2 cut(s) 600, 622
BslFI GGGAC 2 cut(s) 598, 756
BslI CCNNNNNNNGG 4 cut(s) 118, 582, 1015, 1203
BsmAI GTCTC 2 cut(s) 515, 672
BsmFI GGGAC 2 cut(s) 598, 756
BsmI GAATGC 1 cut(s) 617
BsnI GGCC 2 cut(s) 122, 1019
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
BspACI CCGC 2 cut(s) 145, 582
BspANI GGCC 2 cut(s) 122, 1019
BspCNI CTCAG 4 cut(s) 334, 836, 1057, 1150
BspLI GGNNCC 3 cut(s) 543, 631, 789
BspMI ACCTGC 1 cut(s) 442
BspPI GGATC 5 cut(s) 146, 445, 815, 953, 1062
BspT107I GGYRCC 2 cut(s) 541, 787
BspTI CTTAAG 1 cut(s) 431
BsrI ACTGG 1 cut(s) 1307
BssECI CCNNGG 1 cut(s) 1202
BssMI GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
Bst4CI ACNGT 6 cut(s) 264, 352, 577, 747, 1077, 1337
Bst6I CTCTTC 4 cut(s) 783, 1112, 1245, 1334
BstAFI CTTAAG 1 cut(s) 431
BstC8I GCNNGC 1 cut(s) 973
BstDEI CTNAG 6 cut(s) 148, 185, 342, 823, 1065, 1158
BstDSI CCRYGG 1 cut(s) 1202
BstF5I GGATG 5 cut(s) 487, 994, 1097, 1195, 1218
BstKTI GATC 7 cut(s) 154, 440, 639, 810, 961, 1057, 1071
BstMAI GTCTC 2 cut(s) 515, 672
BstMBI GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
BstMWI GCNNNNNNNGC 1 cut(s) 981
BstSFI CTRYAG 1 cut(s) 1333
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 2 cut(s) 914, 933
BstX2I RGATCY 4 cut(s) 151, 437, 958, 1068
BstXI CCANNNNNNTGG 1 cut(s) 552
BstYI RGATCY 4 cut(s) 151, 437, 958, 1068
BsuRI GGCC 2 cut(s) 122, 1019
BtgI CCRYGG 1 cut(s) 1202
BtrI CACGTC 1 cut(s) 1113
BtsCI GGATG 5 cut(s) 487, 994, 1097, 1195, 1218
BtsIMutI CAGTG 1 cut(s) 613
BveI ACCTGC 1 cut(s) 442
Cac8I GCNNGC 1 cut(s) 973
CaiI CAGNNNCTG 1 cut(s) 356
Cfr13I GGNCC 3 cut(s) 629, 847, 1289
Csp6I GTAC 5 cut(s) 83, 272, 820, 1303, 1324
CviQI GTAC 5 cut(s) 83, 272, 820, 1303, 1324
DdeI CTNAG 6 cut(s) 148, 185, 342, 823, 1065, 1158
DpnI GATC 7 cut(s) 153, 439, 638, 809, 960, 1056, 1070
DpnII GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
EaeI YGGCCR 2 cut(s) 120, 1017
Eam1104I CTCTTC 4 cut(s) 783, 1112, 1245, 1334
EarI CTCTTC 4 cut(s) 783, 1112, 1245, 1334
Ecl136II GAGCTC 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 348
Eco32I GATATC 1 cut(s) 298
Eco47I GGWCC 3 cut(s) 629, 847, 1289
Eco53kI GAGCTC 1 cut(s) 346
Eco57I CTGAAG 1 cut(s) 1269
EcoICRI GAGCTC 1 cut(s) 346
EcoRV GATATC 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 348
FalI AAGNNNNNCTT 2 cut(s) 1313, 1345
FaqI GGGAC 2 cut(s) 598, 756
FauI CCCGC 1 cut(s) 575
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 5 cut(s) 494, 1001, 1084, 1202, 1205
FriOI GRGCYC 1 cut(s) 348
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 3 cut(s) 768, 1010, 1139
GluI GCNGC 1 cut(s) 357
GsaI CCCAGC 1 cut(s) 743
GsuI CTGGAG 2 cut(s) 677, 955
HaeIII GGCC 2 cut(s) 122, 1019
HapII CCGG 2 cut(s) 600, 622
HincII GTYRAC 1 cut(s) 1312
HindII GTYRAC 1 cut(s) 1312
HinfI GANTC 3 cut(s) 5, 425, 603
HpaII CCGG 2 cut(s) 600, 622
HphI GGTGA 4 cut(s) 162, 506, 560, 565
Hpy166II GTNNAC 4 cut(s) 46, 83, 1132, 1312
Hpy188I TCNGA 9 cut(s) 295, 343, 697, 812, 826, 988, 1059, 1117, 1159
Hpy188III TCNNGA 1 cut(s) 1080
Hpy8I GTNNAC 4 cut(s) 46, 83, 1132, 1312
HpyAV CCTTC 5 cut(s) 389, 575, 887, 955, 1223
HpyCH4III ACNGT 6 cut(s) 264, 352, 577, 747, 1077, 1337
HpyCH4IV ACGT 1 cut(s) 1112
HpyCH4V TGCA 5 cut(s) 306, 451, 517, 710, 872
HpyF10VI GCNNNNNNNGC 1 cut(s) 981
HpyF3I CTNAG 6 cut(s) 148, 185, 342, 823, 1065, 1158
HpySE526I ACGT 1 cut(s) 1112
Kzo9I GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 1 cut(s) 762
MaeI CTAG 3 cut(s) 768, 1010, 1139
MaeII ACGT 1 cut(s) 1112
MaeIII GTNAC 3 cut(s) 197, 371, 664
MalI GATC 7 cut(s) 153, 439, 638, 809, 960, 1056, 1070
MboI GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
MboII GAAGA 6 cut(s) 770, 914, 933, 1099, 1262, 1321
MflI RGATCY 4 cut(s) 151, 437, 958, 1068
MhlI GDGCHC 1 cut(s) 348
MlsI TGGCCA 2 cut(s) 122, 1019
MluCI AATT 7 cut(s) 113, 684, 771, 874, 906, 963, 977
MluNI TGGCCA 2 cut(s) 122, 1019
MmeI TCCRAC 2 cut(s) 132, 283
Mox20I TGGCCA 2 cut(s) 122, 1019
MscI TGGCCA 2 cut(s) 122, 1019
MseI TTAA 5 cut(s) 432, 801, 909, 927, 1354
MslI CAYNNNNRTG 1 cut(s) 419
Msp20I TGGCCA 2 cut(s) 122, 1019
MspA1I CMGCKG 1 cut(s) 655
MspCI CTTAAG 1 cut(s) 431
MspI CCGG 2 cut(s) 600, 622
Mva1269I GAATGC 1 cut(s) 617
MwoI GCNNNNNNNGC 1 cut(s) 981
NdeII GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
NlaIV GGNNCC 3 cut(s) 543, 631, 789
NmuCI GTSAC 2 cut(s) 371, 664
PctI GAATGC 1 cut(s) 617
PfeI GAWTC 2 cut(s) 425, 603
PflFI GACNNNGTC 2 cut(s) 369, 589
PflMI CCANNNNNTGG 1 cut(s) 118
PkrI GCNGC 1 cut(s) 358
PsiI TTATAA 1 cut(s) 905
Psp124BI GAGCTC 1 cut(s) 348
PspFI CCCAGC 1 cut(s) 739
PspN4I GGNNCC 3 cut(s) 543, 631, 789
PspPI GGNCC 3 cut(s) 629, 847, 1289
PstNI CAGNNNCTG 1 cut(s) 356
PsuI RGATCY 4 cut(s) 151, 437, 958, 1068
PsyI GACNNNGTC 2 cut(s) 369, 589
PvuII CAGCTG 1 cut(s) 655
RsaI GTAC 5 cut(s) 84, 273, 821, 1304, 1325
RsaNI GTAC 5 cut(s) 83, 272, 820, 1303, 1324
RseI CAYNNNNRTG 1 cut(s) 419
SacI GAGCTC 1 cut(s) 348
SaqAI TTAA 5 cut(s) 432, 801, 909, 927, 1354
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 7 cut(s) 151, 437, 636, 807, 958, 1054, 1068
Sau96I GGNCC 3 cut(s) 629, 847, 1289
ScaI AGTACT 1 cut(s) 273
SduI GDGCHC 1 cut(s) 348
SfaNI GCATC 1 cut(s) 762
SfcI CTRYAG 1 cut(s) 1333
SinI GGWCC 3 cut(s) 629, 847, 1289
SmiMI CAYNNNNRTG 1 cut(s) 419
SmlI CTYRAG 3 cut(s) 202, 393, 431
SmoI CTYRAG 3 cut(s) 202, 393, 431
Sse9I AATT 7 cut(s) 113, 684, 771, 874, 906, 963, 977
SsiI CCGC 2 cut(s) 145, 582
SspI AATATT 1 cut(s) 511
SspMI CTAG 3 cut(s) 768, 1010, 1139
SstI GAGCTC 1 cut(s) 348
TaaI ACNGT 6 cut(s) 264, 352, 577, 747, 1077, 1337
TaiI ACGT 1 cut(s) 1115
TaqI TCGA 1 cut(s) 403
TasI AATT 7 cut(s) 113, 684, 771, 874, 906, 963, 977
TatI WGTACW 2 cut(s) 271, 819
TfiI GAWTC 2 cut(s) 425, 603
Tru1I TTAA 5 cut(s) 432, 801, 909, 927, 1354
Tru9I TTAA 5 cut(s) 432, 801, 909, 927, 1354
TscAI CASTG 1 cut(s) 613
TseFI GTSAC 2 cut(s) 371, 664
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 2 cut(s) 371, 664
TspDTI ATGAA 4 cut(s) 61, 446, 867, 1139
TspRI CASTG 1 cut(s) 613
Tth111I GACNNNGTC 2 cut(s) 369, 589
Van91I CCANNNNNTGG 1 cut(s) 118
Vha464I CTTAAG 1 cut(s) 431
VpaK11BI GGWCC 3 cut(s) 629, 847, 1289
XapI RAATTY 2 cut(s) 771, 874
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 3 cut(s) 768, 1010, 1139
ZrmI AGTACT 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.