Rroxscaffold_6G00397930

Cytokinin dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012819
Physical Location & Seq
Forward (+)
19774216 .. 19776084
1869 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00397930.1

Sequence Viewer

Length: 1569 bp
ATGCACTACCTCTCACCTCTCCTTTTCCTCCCACTGTCAGACCATAGGCTCATAACACTACAATATGGACTCACCTCCATACAGCTTCCACAAACAGAACAACATGGTCTACCTCAAACTTTGCTTGATTTTATTCATGGGTTGTGTACCAGATACAACCAACTTCTGTTCCAACCAATTTCTGGCCACCCCAACCATTATTCCATACCGCTTAGATCCTCTCATATCACTTCACCTTTTCAAACACTAAACCTAGATGGTTACTTGAGTTTTGACAACAACCATCATACAGCAATGGACTTTGGCAACATATACCATTTCCAACCATCAGCAGTACTATATCCAAAATCTGTTTCTGATATCTCCTGCATAATAAAGCTTATATTTGAAATGGGTTCTGGTTCTGAGCTTACAGTTGCTGCCAGAGGACATGGTCACTCCCTTCAAGGTCAAGCTCAAGCTCATCGAGGCATAGTCATCAACATGGAATCACTTAAGGGATCTCCAGAAATGCAGGTTCATATTGGAAAGCTACCTTATGTGGATGTTTCGGGTGGTGAGTTATGGATAAATATTCTGCATGAGACGCTCAAACATGGGTTGGCACCAAAATCTTGGACAGATTACCTTCACCTCACCGTTGGCGGGACTTTGTCAAATGCCGGAATCAGTGGTCAGGCATTCCAGCATGGACCCCAGATCAACAATGTCTATCAGCTGGAGGTTGTCACAGGAAAAGGAGACATAATTACCTGTTCCGGAAAGAAAAATGCAGACCTTTTCTATGCTGTTCTGGGAGGCCTGGGACAGTTTGGCATCATTACAAAGGCTAGAATTTCTCTTCAAGCGGCACCAAAGATGGTTAAGTGGATCAGAGTGCTGTACTCAGACTTCTCATTATTTTCAGAGGACCAAGAGCTTCTCATATCATCTGCAAATTCATTCGACTACATTGAAGGATTTGTTATAATTAACAGAACAGGTCTTCTTTATAGCTGGAGGTCTTCCTTCAATCCTAAAGATCCAATTCAAGCGAGCCAATTTAACTCTGATGGTAAAACACTCTACTGCCTAGAAATGGCCAAATACTATAACCCAGAAGAAACTGATGTCATGGATCAGGTGAGACACTCCAAGCGAAAAAAAGAAAAGAAAAGGAAAATATATATGTGTGTGTGTGTATATATATATATATATATCCTCTTATCTTCCGATGATACAAGACGATATTCAATTACTAAAGTACTTGTGCAAATCTTTGTGTATTTGCAGAGAACTGAGATCTTACTGTCAGGATTACAATATATCCCATCCACCCTCTTCACGTCAGAAGTTTCCTTTGTAAACTTCCTAGATAGAGTACATTTGTCTGAGAGGAAACTACGAGAGAAAGGATTATGGGATGTTCCCCACCCGTGGATAAACCTTCTCATCCCTAAAAGCAAGATACATGAGTTTGCTGAAGAGGTCTTTGGCAACATTCTCACAGCCAATATCAATGGTCCTATCCTCATGTACCCAGTCAGCAAAACCAAGTACCTCTTCTACGGTTTTATGACTTCTTTTTAA

Protein Analysis

522

Amino Acids

59.22

Weight (kDa)

8.32

Isoelectric Point (pI)

42.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 109 - 253 1.9e-17 FAD binding domain
Cytokin-bind PF09265 285 - 377 5.3e-27 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Cytokin-bind PF09265 424 - 513 9.4e-34 Cytokinin dehydrogenase 1, FAD and cytokinin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41510 AT2G41510 AT2G41510
fragaria_vesca FvH4_6g24620
malus_domestica MD12G1083600.v1.1 MD14G1078600.v1.1
prunus_persica Prupe.7G052300_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0483951
rosa_laevigata RLG00000023246
rosa_multiflora Rmu_sc0003477.1_g000016
rosa_roxburghii Rroxscaffold_6G00397930
rosa_rugosa Rorug03G0209600 Rorug03G0209700
rosa_samantha Rh3AG257900 Rh3BG295000 Rh3CG292400 Rh3DG288000
rosa_wichuraiana Rw3G023300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 968
Acc36I ACCTGC 1 cut(s) 505
AccB1I GGYRCC 2 cut(s) 604, 850
AccB7I CCANNNNNTGG 1 cut(s) 182
AccI GTMKAC 1 cut(s) 109
AccIII TCCGGA 1 cut(s) 758
AciI CCGC 3 cut(s) 209, 645, 848
AclWI GGATC 5 cut(s) 210, 508, 878, 1016, 1125
AcoI YGGCCR 2 cut(s) 184, 1080
AcsI RAATTY 2 cut(s) 834, 937
AcuI CTGAAG 1 cut(s) 1482
AfaI GTAC 7 cut(s) 148, 336, 884, 1245, 1362, 1517, 1538
AfiI CCNNNNNNNGG 4 cut(s) 182, 645, 1078, 1416
AflII CTTAAG 1 cut(s) 494
AgsI TTSAA 8 cut(s) 242, 389, 446, 845, 956, 1012, 1031, 1233
AjiI CACGTC 1 cut(s) 1326
AjnI CCWGG 1 cut(s) 801
AjuI GAANNNNNNNTTGG 6 cut(s) 337, 369, 1455, 1487, 1526, 1558
AluBI AGCT 9 cut(s) 85, 379, 409, 455, 461, 532, 718, 919, 996
AluI AGCT 9 cut(s) 85, 379, 409, 455, 461, 532, 718, 919, 996
Alw26I GTCTC 3 cut(s) 578, 735, 1120
AlwI GGATC 5 cut(s) 210, 508, 878, 1016, 1125
AlwNI CAGNNNCTG 1 cut(s) 419
Aor13HI TCCGGA 1 cut(s) 758
AoxI GGCC 3 cut(s) 184, 799, 1080
ApeKI GCWGC 1 cut(s) 419
ApoI RAATTY 2 cut(s) 834, 937
AspS9I GGNCC 3 cut(s) 692, 910, 1502
AsuHPI GGTGA 7 cut(s) 6, 64, 225, 569, 623, 628, 1135
AvaII GGWCC 3 cut(s) 692, 910, 1502
BaeI ACNNNNGTAYC 2 cut(s) 1439, 1472
BalI TGGCCA 2 cut(s) 186, 1082
BanI GGYRCC 2 cut(s) 604, 850
BarI GAAGNNNNNNTAC 2 cut(s) 875, 907
BbsI GAAGAC 2 cut(s) 977, 996
BbvI GCAGC 1 cut(s) 406
BccI CCATC 6 cut(s) 251, 291, 334, 853, 1046, 1318
BciT130I CCWGG 1 cut(s) 803
BcoDI GTCTC 3 cut(s) 578, 735, 1120
BfaI CTAG 4 cut(s) 254, 831, 1073, 1352
BfrI CTTAAG 1 cut(s) 494
BfuAI ACCTGC 1 cut(s) 505
BglII AGATCT 1 cut(s) 1281
BisI GCNGC 2 cut(s) 420, 849
BlsI GCNGC 2 cut(s) 421, 850
BmcAI AGTACT 2 cut(s) 336, 1245
Bme1390I CCNGG 1 cut(s) 803
Bme18I GGWCC 3 cut(s) 692, 910, 1502
BmgBI CACGTC 1 cut(s) 1326
BmgT120I GGNCC 3 cut(s) 692, 910, 1502
BmiI GGNNCC 3 cut(s) 606, 694, 852
BmrFI CCNGG 1 cut(s) 803
BmrI ACTGGG 1 cut(s) 1514
BmsI GCATC 1 cut(s) 825
BmuI ACTGGG 1 cut(s) 1514
BpiI GAAGAC 2 cut(s) 977, 996
BpmI CTGGAG 3 cut(s) 489, 740, 1018
BpuEI CTTGAG 2 cut(s) 286, 441
BsaJI CCNNGG 2 cut(s) 802, 1415
BsaWI WCCGGW 1 cut(s) 758
Bsc4I CCNNNNNNNGG 4 cut(s) 182, 645, 1078, 1416
Bse1I ACTGG 1 cut(s) 1520
Bse3DI GCAATG 1 cut(s) 300
BseAI TCCGGA 1 cut(s) 758
BseBI CCWGG 1 cut(s) 803
BseDI CCNNGG 2 cut(s) 802, 1415
BseGI GGATG 4 cut(s) 550, 1310, 1408, 1431
BseLI CCNNNNNNNGG 4 cut(s) 182, 645, 1078, 1416
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 4 cut(s) 396, 900, 1269, 1362
BseNI ACTGG 1 cut(s) 1520
BseXI GCAGC 1 cut(s) 406
BshFI GGCC 3 cut(s) 186, 801, 1082
BshNI GGYRCC 2 cut(s) 604, 850
BsiSI CCGG 2 cut(s) 663, 759
BslFI GGGAC 2 cut(s) 661, 819
BslI CCNNNNNNNGG 4 cut(s) 182, 645, 1078, 1416
BsmAI GTCTC 3 cut(s) 578, 735, 1120
BsmBI CGTCTC 1 cut(s) 578
BsmFI GGGAC 2 cut(s) 661, 819
BsmI GAATGC 1 cut(s) 680
BsnI GGCC 3 cut(s) 186, 801, 1082
Bsp13I TCCGGA 1 cut(s) 758
Bsp143I GATC 7 cut(s) 215, 500, 699, 870, 1021, 1117, 1281
BspACI CCGC 3 cut(s) 209, 645, 848
BspANI GGCC 3 cut(s) 186, 801, 1082
BspCNI CTCAG 4 cut(s) 397, 899, 1270, 1363
BspEI TCCGGA 1 cut(s) 758
BspLI GGNNCC 3 cut(s) 606, 694, 852
BspMI ACCTGC 1 cut(s) 505
BspPI GGATC 5 cut(s) 210, 508, 878, 1016, 1125
BspT107I GGYRCC 2 cut(s) 604, 850
BspTI CTTAAG 1 cut(s) 494
BsrDI GCAATG 1 cut(s) 300
BsrI ACTGG 1 cut(s) 1520
BssECI CCNNGG 2 cut(s) 802, 1415
BssMI GATC 7 cut(s) 215, 500, 699, 870, 1021, 1117, 1281
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 6 cut(s) 36, 415, 640, 810, 1290, 1550
Bst6I CTCTTC 4 cut(s) 846, 1325, 1458, 1547
BstAFI CTTAAG 1 cut(s) 494
BstC8I GCNNGC 1 cut(s) 1036
BstDEI CTNAG 5 cut(s) 212, 405, 886, 1278, 1371
BstDSI CCRYGG 1 cut(s) 1415
BstF5I GGATG 4 cut(s) 550, 1310, 1408, 1431
BstKTI GATC 7 cut(s) 218, 503, 702, 873, 1024, 1120, 1284
BstMAI GTCTC 3 cut(s) 578, 735, 1120
BstMBI GATC 7 cut(s) 215, 500, 699, 870, 1021, 1117, 1281
BstMWI GCNNNNNNNGC 1 cut(s) 586
BstNI CCWGG 1 cut(s) 803
BstSCI CCNGG 1 cut(s) 801
BstV1I GCAGC 1 cut(s) 406
BstV2I GAAGAC 2 cut(s) 977, 996
BstX2I RGATCY 4 cut(s) 215, 500, 1021, 1281
BstXI CCANNNNNNTGG 1 cut(s) 615
BstYI RGATCY 4 cut(s) 215, 500, 1021, 1281
BsuRI GGCC 3 cut(s) 186, 801, 1082
BtgI CCRYGG 1 cut(s) 1415
BtrI CACGTC 1 cut(s) 1326
BtsCI GGATG 4 cut(s) 550, 1310, 1408, 1431
BtsIMutI CAGTG 2 cut(s) 32, 676
BveI ACCTGC 1 cut(s) 505
Cac8I GCNNGC 1 cut(s) 1036
CaiI CAGNNNCTG 1 cut(s) 419
Cfr13I GGNCC 3 cut(s) 692, 910, 1502
CseI GACGC 1 cut(s) 595
Csp6I GTAC 7 cut(s) 147, 335, 883, 1244, 1361, 1516, 1537
CviQI GTAC 7 cut(s) 147, 335, 883, 1244, 1361, 1516, 1537
DdeI CTNAG 5 cut(s) 212, 405, 886, 1278, 1371
DpnI GATC 7 cut(s) 217, 502, 701, 872, 1023, 1119, 1283
DpnII GATC 7 cut(s) 215, 500, 699, 870, 1021, 1117, 1281
EaeI YGGCCR 2 cut(s) 184, 1080
Eam1104I CTCTTC 4 cut(s) 846, 1325, 1458, 1547
EarI CTCTTC 4 cut(s) 846, 1325, 1458, 1547
Eco147I AGGCCT 1 cut(s) 801
Eco32I GATATC 1 cut(s) 361
Eco47I GGWCC 3 cut(s) 692, 910, 1502
Eco57I CTGAAG 1 cut(s) 1482
EcoRII CCWGG 1 cut(s) 801
EcoRV GATATC 1 cut(s) 361
Esp3I CGTCTC 1 cut(s) 578
FalI AAGNNNNNCTT 2 cut(s) 1526, 1558
FaqI GGGAC 2 cut(s) 661, 819
FauI CCCGC 1 cut(s) 638
FblI GTMKAC 1 cut(s) 109
Fnu4HI GCNGC 2 cut(s) 420, 849
FokI GGATG 4 cut(s) 557, 1297, 1415, 1418
Fsp4HI GCNGC 2 cut(s) 420, 849
FspBI CTAG 4 cut(s) 254, 831, 1073, 1352
GluI GCNGC 2 cut(s) 420, 849
GsuI CTGGAG 3 cut(s) 489, 740, 1018
HaeIII GGCC 3 cut(s) 186, 801, 1082
HapII CCGG 2 cut(s) 663, 759
HgaI GACGC 1 cut(s) 595
HindIII AAGCTT 1 cut(s) 377
HinfI GANTC 3 cut(s) 69, 488, 666
HpaII CCGG 2 cut(s) 663, 759
HphI GGTGA 7 cut(s) 6, 64, 225, 569, 623, 628, 1135
Hpy166II GTNNAC 3 cut(s) 110, 147, 1345
Hpy188III TCNNGA 3 cut(s) 506, 759, 1293
Hpy8I GTNNAC 3 cut(s) 110, 147, 1345
HpyAV CCTTC 5 cut(s) 452, 638, 950, 1018, 1436
HpyCH4III ACNGT 6 cut(s) 36, 415, 640, 810, 1290, 1550
HpyCH4IV ACGT 1 cut(s) 1325
HpyCH4V TGCA 8 cut(s) 4, 369, 514, 580, 773, 935, 1252, 1270
HpyF10VI GCNNNNNNNGC 1 cut(s) 586
HpyF3I CTNAG 5 cut(s) 212, 405, 886, 1278, 1371
HpySE526I ACGT 1 cut(s) 1325
Kpn2I TCCGGA 1 cut(s) 758
Kzo9I GATC 7 cut(s) 215, 500, 699, 870, 1021, 1117, 1281
Lsp1109I GCAGC 1 cut(s) 406
LweI GCATC 1 cut(s) 825
MaeI CTAG 4 cut(s) 254, 831, 1073, 1352
MaeII ACGT 1 cut(s) 1325
MaeIII GTNAC 3 cut(s) 260, 434, 727
MalI GATC 7 cut(s) 217, 502, 701, 872, 1023, 1119, 1283
MboI GATC 7 cut(s) 215, 500, 699, 870, 1021, 1117, 1281
MboII GAAGA 8 cut(s) 833, 977, 996, 1112, 1200, 1312, 1475, 1534
MflI RGATCY 4 cut(s) 215, 500, 1021, 1281
MlsI TGGCCA 2 cut(s) 186, 1082
MluCI AATT 8 cut(s) 177, 747, 834, 937, 969, 1026, 1040, 1233
MluNI TGGCCA 2 cut(s) 186, 1082
MlyI GAGTC 1 cut(s) 63
MmeI TCCRAC 2 cut(s) 196, 346
Mox20I TGGCCA 2 cut(s) 186, 1082
MroI TCCGGA 1 cut(s) 758
MscI TGGCCA 2 cut(s) 186, 1082
MseI TTAA 5 cut(s) 495, 864, 972, 1044, 1567
MslI CAYNNNNRTG 1 cut(s) 482
Msp20I TGGCCA 2 cut(s) 186, 1082
MspA1I CMGCKG 1 cut(s) 718
MspCI CTTAAG 1 cut(s) 494
MspI CCGG 2 cut(s) 663, 759
MspR9I CCNGG 1 cut(s) 803
Mva1269I GAATGC 1 cut(s) 680
MvaI CCWGG 1 cut(s) 803
MwoI GCNNNNNNNGC 1 cut(s) 586
NdeII GATC 7 cut(s) 215, 500, 699, 870, 1021, 1117, 1281
NlaIV GGNNCC 3 cut(s) 606, 694, 852
NmuCI GTSAC 2 cut(s) 434, 727
PceI AGGCCT 1 cut(s) 801
PctI GAATGC 1 cut(s) 680
PfeI GAWTC 2 cut(s) 488, 666
PflFI GACNNNGTC 2 cut(s) 432, 652
PflMI CCANNNNNTGG 1 cut(s) 182
PkrI GCNGC 2 cut(s) 421, 850
PleI GAGTC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 63
PsiI TTATAA 1 cut(s) 968
Psp6I CCWGG 1 cut(s) 801
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 3 cut(s) 606, 694, 852
PspPI GGNCC 3 cut(s) 692, 910, 1502
PstNI CAGNNNCTG 1 cut(s) 419
PsuI RGATCY 4 cut(s) 215, 500, 1021, 1281
PsyI GACNNNGTC 2 cut(s) 432, 652
PvuII CAGCTG 1 cut(s) 718
RsaI GTAC 7 cut(s) 148, 336, 884, 1245, 1362, 1517, 1538
RsaNI GTAC 7 cut(s) 147, 335, 883, 1244, 1361, 1516, 1537
RseI CAYNNNNRTG 1 cut(s) 482
SaqAI TTAA 5 cut(s) 495, 864, 972, 1044, 1567
SatI GCNGC 2 cut(s) 420, 849
Sau3AI GATC 7 cut(s) 215, 500, 699, 870, 1021, 1117, 1281
Sau96I GGNCC 3 cut(s) 692, 910, 1502
ScaI AGTACT 2 cut(s) 336, 1245
SchI GAGTC 1 cut(s) 63
ScrFI CCNGG 1 cut(s) 803
SfaNI GCATC 1 cut(s) 825
SinI GGWCC 3 cut(s) 692, 910, 1502
SmiMI CAYNNNNRTG 1 cut(s) 482
SmlI CTYRAG 3 cut(s) 265, 456, 494
SmoI CTYRAG 3 cut(s) 265, 456, 494
Sse9I AATT 8 cut(s) 177, 747, 834, 937, 969, 1026, 1040, 1233
SseBI AGGCCT 1 cut(s) 801
SsiI CCGC 3 cut(s) 209, 645, 848
SspI AATATT 1 cut(s) 574
SspMI CTAG 4 cut(s) 254, 831, 1073, 1352
StuI AGGCCT 1 cut(s) 801
StyD4I CCNGG 1 cut(s) 801
TaaI ACNGT 6 cut(s) 36, 415, 640, 810, 1290, 1550
TaiI ACGT 1 cut(s) 1328
TaqI TCGA 2 cut(s) 466, 945
TasI AATT 8 cut(s) 177, 747, 834, 937, 969, 1026, 1040, 1233
TatI WGTACW 4 cut(s) 334, 882, 1243, 1360
TauI GCSGC 1 cut(s) 851
TfiI GAWTC 2 cut(s) 488, 666
Tru1I TTAA 5 cut(s) 495, 864, 972, 1044, 1567
Tru9I TTAA 5 cut(s) 495, 864, 972, 1044, 1567
TscAI CASTG 2 cut(s) 39, 676
TseFI GTSAC 2 cut(s) 434, 727
TseI GCWGC 1 cut(s) 419
Tsp45I GTSAC 2 cut(s) 434, 727
TspDTI ATGAA 3 cut(s) 125, 509, 930
TspRI CASTG 2 cut(s) 39, 676
Tth111I GACNNNGTC 2 cut(s) 432, 652
Van91I CCANNNNNTGG 1 cut(s) 182
Vha464I CTTAAG 1 cut(s) 494
VpaK11BI GGWCC 3 cut(s) 692, 910, 1502
XapI RAATTY 2 cut(s) 834, 937
XcmI CCANNNNNNNNNTGG 1 cut(s) 179
XmiI GTMKAC 1 cut(s) 109
XspI CTAG 4 cut(s) 254, 831, 1073, 1352
ZrmI AGTACT 2 cut(s) 336, 1245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.