Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0007696

Physical Location & Seq

Genomic Coordinates Forward (+)

23668476 .. 23670739

Gene Structure 2264 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1143800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1449 bp

ATGAAGCTTGCCCATTTGTTTCTTTTATGTCTGGATCGAGATCACCCCTGTCAGATTGTGCCTCCTTCCATGGCCAAATCTCACCTCAAAGAAGAAGATGAAGTTGTTACCGAAAGACCACACGCTTACAGTTTTGTCTGCTGTTACTCCTACTTCTTCACCTCCATACTTTTGTTTTCCTCACTCTCTACTTTCTCCGAGGCTTCTCGCTCCATCCCAACCACCACCACCACCCTCCTTTCTCTCTCCTTCTCTTCCTCTCTGTCCTCCACCAACCCACCGTCAACCTCCCCTTTAAAACCATCTTCCCACGTTGACACTTCCAAAACCCTCATCACATCTCTCACAATCTCCGCCGCCGCTGCCTTCCTCATTGTTTCAGTCATGGTGGTAATCAGATGCTTCGGATTCAGATCAAAACGCAAGCAAAATCGAATCGATGTCGAAAAGGAGGACCATAATGAAGAAAGTGGGGAGTTCAGCGTGAAGAAATTAAGGTGGGATTGGATAGAGAGATCTACTGATGGCTTCTCCAAGGTGATTGGAACTGGAGGGTTCAGCAATGTGTACTTGGCGCGAAACCCGGACCCTAATTCCGGTGATAGGTTCTGTGCGATTAAGGTCCACAACGGCAGCGAGCGGCTGAGCCGGGTTTTTAAGCAAGAACTCGATATTCTCCTCCGCCTTGAGCACCACCACATCGTCAAGCTTCTTGGCTACTCCGACAACCAAGAGGAGGGTGCTCTGGTTTTCGAGTACGTCGCCTACGGAAATCTCCAAGAGAAGCTCCACGGCGGAGACGCCACCCCAGCGATGCCGTGGAAGAACCGAATGGCAATCGCCTTCCAAATCGCACAAGCATTAGAATACCTCCACGAGAAATGCGCCCTCCAAATCGTTCACATGGACATCAAAGCCTCAAATATCTTGCTCGACGAAAATCTCAACTGCAAGCTCTGCGATTTCGGGTCGGCGAAGATGGGGTTTTCCTCGACGGTCCAACCGCCGTCGTCGATGAGAAATCACGTCTTGACGATGATGGGCTCGCCGGGTTACACCGACCCGCACTATCTCCGAACAGGAATCGCTTCGAAGAAAAACGACGTATACAGCTTCGGCGTTTTGATCTTGGAGCTCGTGACCGGAATGGAGGCATTTTGCTCCGAAAAAGGGCAGTTCTTGACGACGTTGGTGGGGCCCAGGTTGAGGTACGGCGGTTGTGACGCAGCGGAGGTGGCCGAGTTGGTGGACCCGCGGTTGGGCGCGGCGGGGTTTGACGTCGAAGAGGCCAAGACCATGCTTGGTATTTCAGCAATGTGCTTGCGTCAGTCACCGACACTGAGGCCTTCGGCTACTCAAGTATTGGAGACCTTTCGGGACAAAGTTTCGTCTGTTTCGTTACTACAGTCAGCGTCTGATGACATGACAAAGAAATCGTATGCTTGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0006950 GO:0008150 GO:0009611 GO:0050896

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase

Protein Analysis

483

Amino Acids

53.38

Weight (kDa)

6.57

Isoelectric Point (pI)

42.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	177 - 457	9.9e-37	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	179 - 456	1.4e-40	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007696)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G33260 AT1G33260 AT4G10390
fragaria_vesca	FvH4_5g10090
malus_domestica	MD06G1128000.v1.1 MD14G1143800.v1.1
prunus_persica	Prupe.5G137800_v2.0.a1
pyrus_communis	pycom06g11830 pycom14g11920
rosa_chinensis	RchiOBHm_Chr7g0188181
rosa_laevigata	RLG00000004721
rosa_multiflora	Rmu_co8339873.1_g000001 Rmu_sc0004001.1_g000014 Rmu_sc0032708.1_g000001 Rmu_sc0033897.1_g000001
rosa_roxburghii	Rroxscaffold_1G00031530 Rroxscaffold_3G00266690 Rroxscaffold_5G00335880
rosa_rugosa	Rorug06G0489000
rosa_samantha	Rh7AG093200 Rh7BG094700 Rh7CG094500 Rh7DG095100
rosa_wichuraiana	Rw7G008040

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1281
AccBSI	CCGCTC	1 cut(s)	640
AccI	GTMKAC	1 cut(s)	1107
AccII	CGCG	3 cut(s)	577, 1255, 1265
AclWI	GGATC	1 cut(s)	42
AcoI	YGGCCR	2 cut(s)	72, 1236
AcyI	GRCGYC	2 cut(s)	801, 1278
AfaI	GTAC	3 cut(s)	569, 758, 1211
AfiI	CCNNNNNNNGG	7 cut(s)	596, 603, 736, 1170, 1206, 1258, 1259
AjiI	CACGTC	1 cut(s)	1027
AjnI	CCWGG	1 cut(s)	1199
AluBI	AGCT	6 cut(s)	7, 709, 787, 955, 1113, 1135
AluI	AGCT	6 cut(s)	7, 709, 787, 955, 1113, 1135
Alw21I	GWGCWC	3 cut(s)	693, 745, 1137
Alw26I	GTCTC	2 cut(s)	792, 1361
AlwI	GGATC	1 cut(s)	42
AlwNI	CAGNNNCTG	1 cut(s)	1415
AoxI	GGCC	5 cut(s)	72, 1196, 1236, 1287, 1343
ApaI	GGGCCC	1 cut(s)	1200
ApeKI	GCWGC	3 cut(s)	362, 633, 1226
Asp700I	GAANNNNTTC	1 cut(s)	1087
AspLEI	GCGC	3 cut(s)	577, 887, 1265
AspS9I	GGNCC	7 cut(s)	454, 586, 622, 997, 1196, 1197, 1249
AsuC2I	CCSGG	3 cut(s)	584, 650, 1050
AsuHPI	GGTGA	6 cut(s)	35, 74, 151, 550, 611, 1323
AsuII	TTCGAA	1 cut(s)	1091
AvaII	GGWCC	5 cut(s)	454, 586, 622, 997, 1249
BaeGI	GKGCMC	1 cut(s)	1200
BaeI	ACNNNNGTAYC	2 cut(s)	1201, 1234
BalI	TGGCCA	1 cut(s)	74
BanII	GRGCYC	3 cut(s)	1046, 1137, 1200
BauI	CACGAG	2 cut(s)	875, 1136
Bbv12I	GWGCWC	3 cut(s)	693, 745, 1137
BbvI	GCAGC	3 cut(s)	349, 645, 1238
BccI	CCATC	5 cut(s)	221, 310, 518, 973, 1033
BceAI	ACGGC	5 cut(s)	646, 802, 808, 991, 1228
BciT130I	CCWGG	1 cut(s)	1201
BcnI	CCSGG	3 cut(s)	584, 650, 1050
BcoDI	GTCTC	2 cut(s)	792, 1361
BfaI	CTAG	1 cut(s)	1447
BfmI	CTRYAG	1 cut(s)	1403
BglII	AGATCT	1 cut(s)	515
BisI	GCNGC	7 cut(s)	357, 360, 363, 634, 641, 1227, 1266
BlpI	GCTNAGC	1 cut(s)	644
BlsI	GCNGC	7 cut(s)	358, 361, 364, 635, 642, 1228, 1267
Bme1390I	CCNGG	4 cut(s)	584, 650, 1050, 1201
Bme18I	GGWCC	5 cut(s)	454, 586, 622, 997, 1249
BmgBI	CACGTC	1 cut(s)	1027
BmgT120I	GGNCC	7 cut(s)	454, 586, 622, 997, 1196, 1197, 1249
BmiI	GGNNCC	4 cut(s)	588, 1197, 1198, 1251
BmrFI	CCNGG	4 cut(s)	584, 650, 1050, 1201
BmsI	GCATC	2 cut(s)	389, 804
BpmI	CTGGAG	1 cut(s)	570
Bpu1102I	GCTNAGC	1 cut(s)	644
Bpu14I	TTCGAA	1 cut(s)	1091
BpuEI	CTTGAG	2 cut(s)	707, 1341
BpuMI	CCSGG	3 cut(s)	584, 650, 1050
Bsa29I	ATCGAT	1 cut(s)	438
BsaBI	GATNNNNATC	2 cut(s)	39, 412
BsaHI	GRCGYC	2 cut(s)	801, 1278
BsaI	GGTCTC	1 cut(s)	1361
BsaJI	CCNNGG	7 cut(s)	69, 198, 534, 790, 818, 1199, 1253
BsaWI	WCCGGW	2 cut(s)	596, 1142
Bsc4I	CCNNNNNNNGG	7 cut(s)	596, 603, 736, 1170, 1206, 1258, 1259
Bse1I	ACTGG	1 cut(s)	553
Bse3DI	GCAATG	2 cut(s)	568, 1320
Bse8I	GATNNNNATC	2 cut(s)	39, 412
BseBI	CCWGG	1 cut(s)	1201
BseCI	ATCGAT	1 cut(s)	438
BseDI	CCNNGG	7 cut(s)	69, 198, 534, 790, 818, 1199, 1253
BseGI	GGATG	1 cut(s)	213
BseJI	GATNNNNATC	2 cut(s)	39, 412
BseLI	CCNNNNNNNGG	7 cut(s)	596, 603, 736, 1170, 1206, 1258, 1259
BseMI	GCAATG	2 cut(s)	568, 1320
BseMII	CTCAG	2 cut(s)	635, 1331
BseNI	ACTGG	1 cut(s)	553
BseRI	GAGGAG	2 cut(s)	668, 749
BseSI	GKGCMC	1 cut(s)	1200
BseXI	GCAGC	3 cut(s)	349, 645, 1238
BseYI	CCCAGC	1 cut(s)	808
Bsh1236I	CGCG	3 cut(s)	577, 1255, 1265
BshFI	GGCC	5 cut(s)	74, 1198, 1238, 1289, 1345
BshVI	ATCGAT	1 cut(s)	438
BsiHKAI	GWGCWC	3 cut(s)	693, 745, 1137
BsiSI	CCGG	5 cut(s)	584, 597, 649, 1049, 1143
BslFI	GGGAC	1 cut(s)	1391
BslI	CCNNNNNNNGG	7 cut(s)	596, 603, 736, 1170, 1206, 1258, 1259
BsmAI	GTCTC	2 cut(s)	792, 1361
BsmBI	CGTCTC	1 cut(s)	792
BsmFI	GGGAC	1 cut(s)	1391
BsnI	GGCC	5 cut(s)	74, 1198, 1238, 1289, 1345
Bso31I	GGTCTC	1 cut(s)	1361
Bsp119I	TTCGAA	1 cut(s)	1091
Bsp120I	GGGCCC	1 cut(s)	1196
Bsp1286I	GDGCHC	5 cut(s)	693, 745, 1046, 1137, 1200
Bsp143I	GATC	5 cut(s)	34, 40, 413, 515, 1125
Bsp1720I	GCTNAGC	1 cut(s)	644
Bsp19I	CCATGG	1 cut(s)	69
BspANI	GGCC	5 cut(s)	74, 1198, 1238, 1289, 1345
BspCNI	CTCAG	2 cut(s)	636, 1332
BspDI	ATCGAT	1 cut(s)	438
BspFNI	CGCG	3 cut(s)	577, 1255, 1265
BspLI	GGNNCC	4 cut(s)	588, 1197, 1198, 1251
BspPI	GGATC	1 cut(s)	42
BspT104I	TTCGAA	1 cut(s)	1091
BspTNI	GGTCTC	1 cut(s)	1361
BsrBI	CCGCTC	1 cut(s)	640
BsrDI	GCAATG	2 cut(s)	568, 1320
BsrI	ACTGG	1 cut(s)	553
BssECI	CCNNGG	7 cut(s)	69, 198, 534, 790, 818, 1199, 1253
BssMI	GATC	5 cut(s)	34, 40, 413, 515, 1125
BssNAI	GTATAC	1 cut(s)	1108
BssNI	GRCGYC	2 cut(s)	801, 1278
BssSI	CACGAG	2 cut(s)	875, 1136
BssT1I	CCWWGG	2 cut(s)	69, 534
Bst1107I	GTATAC	1 cut(s)	1108
Bst2BI	CACGAG	2 cut(s)	875, 1136
Bst2UI	CCWGG	1 cut(s)	1201
Bst4CI	ACNGT	4 cut(s)	131, 282, 997, 1407
Bst6I	CTCTTC	2 cut(s)	259, 1278
BstACI	GRCGYC	2 cut(s)	801, 1278
BstAPI	GCANNNNNTGC	1 cut(s)	957
BstBI	TTCGAA	1 cut(s)	1091
BstC8I	GCNNGC	7 cut(s)	9, 425, 638, 953, 1046, 1322, 1444
BstDEI	CTNAG	2 cut(s)	644, 1340
BstDSI	CCRYGG	4 cut(s)	69, 790, 818, 1253
BstF5I	GGATG	1 cut(s)	213
BstFNI	CGCG	3 cut(s)	577, 1255, 1265
BstHHI	GCGC	3 cut(s)	577, 887, 1265
BstKTI	GATC	5 cut(s)	37, 43, 416, 518, 1128
BstMAI	GTCTC	2 cut(s)	792, 1361
BstMBI	GATC	5 cut(s)	34, 40, 413, 515, 1125
BstMWI	GCNNNNNNNGC	4 cut(s)	362, 809, 957, 1235
BstNI	CCWGG	1 cut(s)	1201
BstSCI	CCNGG	4 cut(s)	582, 648, 1048, 1199
BstSFI	CTRYAG	1 cut(s)	1403
BstSLI	GKGCMC	1 cut(s)	1200
BstUI	CGCG	3 cut(s)	577, 1255, 1265
BstV1I	GCAGC	3 cut(s)	349, 645, 1238
BstX2I	RGATCY	1 cut(s)	515
BstYI	RGATCY	1 cut(s)	515
BstZ17I	GTATAC	1 cut(s)	1108
Bsu15I	ATCGAT	1 cut(s)	438
BsuRI	GGCC	5 cut(s)	74, 1198, 1238, 1289, 1345
BsuTUI	ATCGAT	1 cut(s)	438
BtgI	CCRYGG	4 cut(s)	69, 790, 818, 1253
BtgZI	GCGATG	1 cut(s)	827
BtrI	CACGTC	1 cut(s)	1027
BtsCI	GGATG	1 cut(s)	213
BtsIMutI	CAGTG	1 cut(s)	1337
Cac8I	GCNNGC	7 cut(s)	9, 425, 638, 953, 1046, 1322, 1444
CaiI	CAGNNNCTG	1 cut(s)	1415
CfoI	GCGC	3 cut(s)	577, 887, 1265
Cfr13I	GGNCC	7 cut(s)	454, 586, 622, 997, 1196, 1197, 1249
Cfr42I	CCGCGG	1 cut(s)	1256
ClaI	ATCGAT	1 cut(s)	438
CseI	GACGC	4 cut(s)	809, 1232, 1313, 1401
Csp6I	GTAC	3 cut(s)	568, 757, 1210
CviAII	CATG	5 cut(s)	70, 385, 904, 1297, 1423
CviQI	GTAC	3 cut(s)	568, 757, 1210
DdeI	CTNAG	2 cut(s)	644, 1340
DpnI	GATC	5 cut(s)	36, 42, 415, 517, 1127
DpnII	GATC	5 cut(s)	34, 40, 413, 515, 1125
DraI	TTTAAA	1 cut(s)	297
EaeI	YGGCCR	2 cut(s)	72, 1236
Eam1104I	CTCTTC	2 cut(s)	259, 1278
EarI	CTCTTC	2 cut(s)	259, 1278
EciI	GGCGGA	3 cut(s)	343, 671, 810
Ecl136II	GAGCTC	1 cut(s)	1135
Eco130I	CCWWGG	2 cut(s)	69, 534
Eco147I	AGGCCT	1 cut(s)	1345
Eco24I	GRGCYC	3 cut(s)	1046, 1137, 1200
Eco31I	GGTCTC	1 cut(s)	1361
Eco47I	GGWCC	5 cut(s)	454, 586, 622, 997, 1249
Eco53kI	GAGCTC	1 cut(s)	1135
EcoICRI	GAGCTC	1 cut(s)	1135
EcoO109I	RGGNCCY	1 cut(s)	1196
EcoRII	CCWGG	1 cut(s)	1199
EcoT14I	CCWWGG	2 cut(s)	69, 534
EcoT38I	GRGCYC	3 cut(s)	1046, 1137, 1200
ErhI	CCWWGG	2 cut(s)	69, 534
Esp3I	CGTCTC	1 cut(s)	792
FaeI	CATG	5 cut(s)	73, 388, 907, 1300, 1426
FaqI	GGGAC	1 cut(s)	1391
FatI	CATG	5 cut(s)	69, 384, 903, 1296, 1422
FauI	CCCGC	3 cut(s)	1071, 1260, 1261
FblI	GTMKAC	1 cut(s)	1107
Fnu4HI	GCNGC	7 cut(s)	357, 360, 363, 634, 641, 1227, 1266
FokI	GGATG	1 cut(s)	200
FriOI	GRGCYC	3 cut(s)	1046, 1137, 1200
Fsp4HI	GCNGC	7 cut(s)	357, 360, 363, 634, 641, 1227, 1266
FspBI	CTAG	1 cut(s)	1447
GlaI	GCGC	3 cut(s)	576, 886, 1264
GluI	GCNGC	7 cut(s)	357, 360, 363, 634, 641, 1227, 1266
GsaI	CCCAGC	1 cut(s)	812
GsuI	CTGGAG	1 cut(s)	570
HaeIII	GGCC	5 cut(s)	74, 1198, 1238, 1289, 1345
HapII	CCGG	5 cut(s)	584, 597, 649, 1049, 1143
HgaI	GACGC	4 cut(s)	809, 1232, 1313, 1401
HhaI	GCGC	3 cut(s)	577, 887, 1265
Hin1I	GRCGYC	2 cut(s)	801, 1278
Hin1II	CATG	5 cut(s)	73, 388, 907, 1300, 1426
Hin6I	GCGC	3 cut(s)	575, 885, 1263
HinP1I	GCGC	3 cut(s)	575, 885, 1263
HincII	GTYRAC	2 cut(s)	285, 316
HindII	GTYRAC	2 cut(s)	285, 316
HindIII	AAGCTT	2 cut(s)	5, 707
HinfI	GANTC	3 cut(s)	408, 435, 1083
HpaII	CCGG	5 cut(s)	584, 597, 649, 1049, 1143
HphI	GGTGA	6 cut(s)	35, 74, 151, 550, 611, 1323
Hpy166II	GTNNAC	7 cut(s)	285, 316, 568, 625, 901, 1108, 1249
Hpy188I	TCNGA	9 cut(s)	54, 199, 398, 407, 413, 724, 1076, 1165, 1417
Hpy188III	TCNNGA	6 cut(s)	32, 38, 1030, 1138, 1180, 1376
Hpy8I	GTNNAC	7 cut(s)	285, 316, 568, 625, 901, 1108, 1249
Hpy99I	CGWCG	8 cut(s)	764, 938, 997, 1012, 1015, 1106, 1189, 1283
HpyAV	CCTTC	5 cut(s)	75, 259, 376, 853, 1356
HpyCH4III	ACNGT	4 cut(s)	131, 282, 997, 1407
HpyCH4IV	ACGT	6 cut(s)	312, 759, 1026, 1104, 1187, 1278
HpyCH4V	TGCA	1 cut(s)	951
HpyF10VI	GCNNNNNNNGC	4 cut(s)	362, 809, 957, 1235
HpyF3I	CTNAG	2 cut(s)	644, 1340
HpySE526I	ACGT	6 cut(s)	312, 759, 1026, 1104, 1187, 1278
Hsp92I	GRCGYC	2 cut(s)	801, 1278
Hsp92II	CATG	5 cut(s)	73, 388, 907, 1300, 1426
HspAI	GCGC	3 cut(s)	575, 885, 1263
KspI	CCGCGG	1 cut(s)	1256
Kzo9I	GATC	5 cut(s)	34, 40, 413, 515, 1125
LmnI	GCTCC	4 cut(s)	215, 792, 1132, 1166
Lsp1109I	GCAGC	3 cut(s)	349, 645, 1238
LweI	GCATC	2 cut(s)	389, 804
MaeI	CTAG	1 cut(s)	1447
MaeII	ACGT	6 cut(s)	312, 759, 1026, 1104, 1187, 1278
MaeIII	GTNAC	7 cut(s)	106, 143, 1052, 1138, 1220, 1329, 1398
MalI	GATC	5 cut(s)	36, 42, 415, 517, 1127
MbiI	CCGCTC	1 cut(s)	640
MboI	GATC	5 cut(s)	34, 40, 413, 515, 1125
MflI	RGATCY	1 cut(s)	515
MhlI	GDGCHC	5 cut(s)	693, 745, 1046, 1137, 1200
MlsI	TGGCCA	1 cut(s)	74
MluCI	AATT	2 cut(s)	491, 592
MluNI	TGGCCA	1 cut(s)	74
MmeI	TCCRAC	2 cut(s)	747, 1024
Mox20I	TGGCCA	1 cut(s)	74
MroXI	GAANNNNTTC	1 cut(s)	1087
MscI	TGGCCA	1 cut(s)	74
MseI	TTAA	4 cut(s)	296, 494, 618, 657
Msp20I	TGGCCA	1 cut(s)	74
MspA1I	CMGCKG	3 cut(s)	362, 1229, 1255
MspI	CCGG	5 cut(s)	584, 597, 649, 1049, 1143
MspR9I	CCNGG	4 cut(s)	584, 650, 1050, 1201
MvaI	CCWGG	1 cut(s)	1201
MvnI	CGCG	3 cut(s)	577, 1255, 1265
MwoI	GCNNNNNNNGC	4 cut(s)	362, 809, 957, 1235
NciI	CCSGG	3 cut(s)	584, 650, 1050
NcoI	CCATGG	1 cut(s)	69
NdeII	GATC	5 cut(s)	34, 40, 413, 515, 1125
NlaIII	CATG	5 cut(s)	73, 388, 907, 1300, 1426
NlaIV	GGNNCC	4 cut(s)	588, 1197, 1198, 1251
NmeAIII	GCCGAG	1 cut(s)	1264
NmuCI	GTSAC	3 cut(s)	1138, 1220, 1329
NspV	TTCGAA	1 cut(s)	1091
PceI	AGGCCT	1 cut(s)	1345
PcsI	WCGNNNNNNNCGW	1 cut(s)	1394
PdmI	GAANNNNTTC	1 cut(s)	1087
PfeI	GAWTC	3 cut(s)	408, 435, 1083
PkrI	GCNGC	7 cut(s)	358, 361, 364, 635, 642, 1228, 1267
Psp124BI	GAGCTC	1 cut(s)	1137
Psp6I	CCWGG	1 cut(s)	1199
PspFI	CCCAGC	1 cut(s)	808
PspGI	CCWGG	1 cut(s)	1199
PspN4I	GGNNCC	4 cut(s)	588, 1197, 1198, 1251
PspOMI	GGGCCC	1 cut(s)	1196
PspPI	GGNCC	7 cut(s)	454, 586, 622, 997, 1196, 1197, 1249
PstNI	CAGNNNCTG	1 cut(s)	1415
PsuI	RGATCY	1 cut(s)	515
RsaI	GTAC	3 cut(s)	569, 758, 1211
RsaNI	GTAC	3 cut(s)	568, 757, 1210
SacI	GAGCTC	1 cut(s)	1137
SacII	CCGCGG	1 cut(s)	1256
SaqAI	TTAA	4 cut(s)	296, 494, 618, 657
SatI	GCNGC	7 cut(s)	357, 360, 363, 634, 641, 1227, 1266
Sau3AI	GATC	5 cut(s)	34, 40, 413, 515, 1125
Sau96I	GGNCC	7 cut(s)	454, 586, 622, 997, 1196, 1197, 1249
ScrFI	CCNGG	4 cut(s)	584, 650, 1050, 1201
SduI	GDGCHC	5 cut(s)	693, 745, 1046, 1137, 1200
SfaNI	GCATC	2 cut(s)	389, 804
SfcI	CTRYAG	1 cut(s)	1403
Sfr303I	CCGCGG	1 cut(s)	1256
SfuI	TTCGAA	1 cut(s)	1091
SgrBI	CCGCGG	1 cut(s)	1256
SinI	GGWCC	5 cut(s)	454, 586, 622, 997, 1249
SmlI	CTYRAG	2 cut(s)	686, 1356
SmoI	CTYRAG	2 cut(s)	686, 1356
Sse9I	AATT	2 cut(s)	491, 592
SseBI	AGGCCT	1 cut(s)	1345
SspMI	CTAG	1 cut(s)	1447
SstI	GAGCTC	1 cut(s)	1137
StuI	AGGCCT	1 cut(s)	1345
StyD4I	CCNGG	4 cut(s)	582, 648, 1048, 1199
StyI	CCWWGG	2 cut(s)	69, 534
TaaI	ACNGT	4 cut(s)	131, 282, 997, 1407
TaiI	ACGT	6 cut(s)	315, 762, 1029, 1107, 1190, 1281
TasI	AATT	2 cut(s)	491, 592
TatI	WGTACW	1 cut(s)	567
TauI	GCSGC	4 cut(s)	359, 362, 643, 1268
TfiI	GAWTC	3 cut(s)	408, 435, 1083
Tru1I	TTAA	4 cut(s)	296, 494, 618, 657
Tru9I	TTAA	4 cut(s)	296, 494, 618, 657
TscAI	CASTG	1 cut(s)	1344
TseFI	GTSAC	3 cut(s)	1138, 1220, 1329
TseI	GCWGC	3 cut(s)	362, 633, 1226
Tsp45I	GTSAC	3 cut(s)	1138, 1220, 1329
TspDTI	ATGAA	3 cut(s)	17, 114, 477
TspGWI	ACGGA	1 cut(s)	783
TspRI	CASTG	1 cut(s)	1344
VpaK11BI	GGWCC	5 cut(s)	454, 586, 622, 997, 1249
XcmI	CCANNNNNNNNNTGG	1 cut(s)	816
XmiI	GTMKAC	1 cut(s)	1107
XmnI	GAANNNNTTC	1 cut(s)	1087
XspI	CTAG	1 cut(s)	1447
ZraI	GACGTC	1 cut(s)	1279

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.