MD14G1144900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015895
Physical Location & Seq
Reverse (-)
23801140 .. 23801882
743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1144900.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGCTTTCAGATCAATGAACCACTGGAAACAAATGTCGACCGGCTTACGCCGATACGCAACCGCGACCTCTCCGAAGATGAAACCCTACGCCCCGGCTGCCGGTGCCACCGAAAATCCCCAACAGAGCTACTTCAAAAGGTTCATGAGGGGCGACTTTGTGCCGGTCTACGTTGCTGTCGGGATGATAGCGCTGTCGGTGGCGCTGGGGCTCCACACCGCCAAGCAGCACCTGAGGTACAACCCCCACGTCTACGTGAAGAAGAACCGGAGAGAGACGCTGCCGGAGGTGGTGGAGCCGGAGCGCGTGGTGGAGGAGTCTGACAAGTTCATTCAAAATTCGTTTTTCAGGAAGGTGGCGCATGTTCAGGAGTTTGACGACTACAATCACGCCGTTAAGGATCCGATCCGGAAAGATGTTTTCGCTCACAAAGCCGAACCGCCCCGTTCGAAAACCGTGACCCTCAAGGACGTCGGAGTAGACCCTATCCGTGCCCAACCCGAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

170

Amino Acids

19.38

Weight (kDa)

9.78

Isoelectric Point (pI)

53.12

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015895)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G07330
fragaria_vesca FvH4_5g09901
malus_domestica MD00G1076100.v1.1 MD14G1144900.v1.1
prunus_persica Prupe.5G139500_v2.0.a1
pyrus_communis pycom06g11960 pycom14g12040
rosa_chinensis RchiOBHm_Chr7g0188371
rosa_laevigata RLG00000004698
rosa_multiflora Rmu_co8426879.1_g000001
rosa_rugosa Rorug06G0490200
rosa_samantha Rh7AG094900 Rh7BG096400 Rh7DG096700
rosa_wichuraiana Rw7G008180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 474
AccB1I GGYRCC 1 cut(s) 104
AccI GTMKAC 4 cut(s) 38, 168, 252, 480
AccII CGCG 2 cut(s) 65, 306
AccIII TCCGGA 1 cut(s) 408
AciI CCGC 3 cut(s) 63, 219, 440
AclWI GGATC 3 cut(s) 395, 400, 408
AcsI RAATTY 1 cut(s) 337
AcyI GRCGYC 1 cut(s) 471
AfaI GTAC 1 cut(s) 239
AfeI AGCGCT 1 cut(s) 192
AfiI CCNNNNNNNGG 1 cut(s) 101
AgsI TTSAA 2 cut(s) 136, 335
AjiI CACGTC 1 cut(s) 250
AluBI AGCT 2 cut(s) 129, 506
AluI AGCT 2 cut(s) 129, 506
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 3 cut(s) 395, 400, 408
AlwNI CAGNNNCTG 1 cut(s) 232
Aor13HI TCCGGA 1 cut(s) 408
Aor51HI AGCGCT 1 cut(s) 192
ApeKI GCWGC 3 cut(s) 98, 226, 280
ApoI RAATTY 1 cut(s) 337
AspLEI GCGC 4 cut(s) 193, 205, 306, 361
AsuC2I CCSGG 1 cut(s) 95
AsuII TTCGAA 1 cut(s) 449
AxyI CCTNAGG 1 cut(s) 233
BaeGI GKGCMC 1 cut(s) 496
BamHI GGATCC 1 cut(s) 400
BanI GGYRCC 1 cut(s) 104
BanII GRGCYC 1 cut(s) 213
BbvI GCAGC 3 cut(s) 85, 238, 267
BceAI ACGGC 1 cut(s) 377
BcnI CCSGG 1 cut(s) 95
BcoDI GTCTC 1 cut(s) 269
BfoI RGCGCY 2 cut(s) 194, 206
BisI GCNGC 3 cut(s) 99, 227, 281
BlsI GCNGC 3 cut(s) 100, 228, 282
Bme1390I CCNGG 1 cut(s) 95
BmgBI CACGTC 1 cut(s) 250
BmiI GGNNCC 4 cut(s) 106, 212, 297, 402
BmrFI CCNGG 1 cut(s) 95
Bpu14I TTCGAA 1 cut(s) 449
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 95
BsaAI YACGTR 1 cut(s) 256
BsaHI GRCGYC 1 cut(s) 471
BsaJI CCNNGG 1 cut(s) 93
BsaWI WCCGGW 2 cut(s) 267, 408
Bsc4I CCNNNNNNNGG 1 cut(s) 101
Bse118I RCCGGY 3 cut(s) 41, 101, 163
Bse1I ACTGG 1 cut(s) 29
Bse21I CCTNAGG 1 cut(s) 233
BseAI TCCGGA 1 cut(s) 408
BseDI CCNNGG 1 cut(s) 93
BseGI GGATG 1 cut(s) 189
BseLI CCNNNNNNNGG 1 cut(s) 101
BseMII CTCAG 1 cut(s) 224
BseNI ACTGG 1 cut(s) 29
BseRI GAGGAG 1 cut(s) 329
BseSI GKGCMC 1 cut(s) 496
BseXI GCAGC 3 cut(s) 85, 238, 267
BseYI CCCAGC 1 cut(s) 205
Bsh1236I CGCG 2 cut(s) 65, 306
Bsh1285I CGRYCG 1 cut(s) 42
BshNI GGYRCC 1 cut(s) 104
BsiEI CGRYCG 1 cut(s) 42
BsiSI CCGG 8 cut(s) 42, 95, 102, 164, 268, 284, 299, 409
BslI CCNNNNNNNGG 1 cut(s) 101
BsmAI GTCTC 1 cut(s) 269
BsmBI CGTCTC 1 cut(s) 269
Bsp119I TTCGAA 1 cut(s) 449
Bsp1286I GDGCHC 2 cut(s) 213, 496
Bsp13I TCCGGA 1 cut(s) 408
Bsp143I GATC 3 cut(s) 11, 400, 405
BspACI CCGC 3 cut(s) 63, 219, 440
BspCNI CTCAG 1 cut(s) 225
BspEI TCCGGA 1 cut(s) 408
BspFNI CGCG 2 cut(s) 65, 306
BspHI TCATGA 1 cut(s) 144
BspLI GGNNCC 4 cut(s) 106, 212, 297, 402
BspPI GGATC 3 cut(s) 395, 400, 408
BspT104I TTCGAA 1 cut(s) 449
BspT107I GGYRCC 1 cut(s) 104
BsrFI RCCGGY 3 cut(s) 41, 101, 163
BsrI ACTGG 1 cut(s) 29
BssAI RCCGGY 3 cut(s) 41, 101, 163
BssECI CCNNGG 1 cut(s) 93
BssMI GATC 3 cut(s) 11, 400, 405
BssNI GRCGYC 1 cut(s) 471
Bst4CI ACNGT 1 cut(s) 457
BstACI GRCGYC 1 cut(s) 471
BstBAI YACGTR 1 cut(s) 256
BstBI TTCGAA 1 cut(s) 449
BstDEI CTNAG 2 cut(s) 233, 507
BstF5I GGATG 1 cut(s) 189
BstFNI CGCG 2 cut(s) 65, 306
BstH2I RGCGCY 2 cut(s) 194, 206
BstHHI GCGC 4 cut(s) 193, 205, 306, 361
BstKTI GATC 3 cut(s) 14, 403, 408
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 3 cut(s) 11, 400, 405
BstMCI CGRYCG 1 cut(s) 42
BstMWI GCNNNNNNNGC 3 cut(s) 98, 104, 431
BstNSI RCATGY 1 cut(s) 365
BstSCI CCNGG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 496
BstUI CGCG 2 cut(s) 65, 306
BstV1I GCAGC 3 cut(s) 85, 238, 267
BstX2I RGATCY 1 cut(s) 400
BstYI RGATCY 1 cut(s) 400
Bsu36I CCTNAGG 1 cut(s) 233
BtrI CACGTC 1 cut(s) 250
BtsCI GGATG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 22
CaiI CAGNNNCTG 1 cut(s) 232
CciI TCATGA 1 cut(s) 144
CfoI GCGC 4 cut(s) 193, 205, 306, 361
Cfr10I RCCGGY 3 cut(s) 41, 101, 163
CseI GACGC 1 cut(s) 286
Csp6I GTAC 1 cut(s) 238
CviAII CATG 2 cut(s) 145, 362
CviJI RGCY 8 cut(s) 5, 45, 98, 129, 211, 298, 434, 506
CviKI_1 RGCY 8 cut(s) 5, 45, 98, 129, 211, 298, 434, 506
CviQI GTAC 1 cut(s) 238
DdeI CTNAG 2 cut(s) 233, 507
DpnI GATC 3 cut(s) 13, 402, 407
DpnII GATC 3 cut(s) 11, 400, 405
Eco24I GRGCYC 1 cut(s) 213
Eco47III AGCGCT 1 cut(s) 192
Eco81I CCTNAGG 1 cut(s) 233
EcoT38I GRGCYC 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 269
FaeI CATG 2 cut(s) 148, 365
FaiI YATR 2 cut(s) 146, 363
FatI CATG 2 cut(s) 144, 361
FblI GTMKAC 4 cut(s) 38, 168, 252, 480
Fnu4HI GCNGC 3 cut(s) 99, 227, 281
FokI GGATG 1 cut(s) 196
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 3 cut(s) 99, 227, 281
GlaI GCGC 4 cut(s) 192, 204, 305, 360
GluI GCNGC 3 cut(s) 99, 227, 281
GsaI CCCAGC 1 cut(s) 209
HaeII RGCGCY 2 cut(s) 194, 206
HapII CCGG 8 cut(s) 42, 95, 102, 164, 268, 284, 299, 409
HgaI GACGC 1 cut(s) 286
HhaI GCGC 4 cut(s) 193, 205, 306, 361
Hin1I GRCGYC 1 cut(s) 471
Hin1II CATG 2 cut(s) 148, 365
Hin6I GCGC 4 cut(s) 191, 203, 304, 359
HinP1I GCGC 4 cut(s) 191, 203, 304, 359
HincII GTYRAC 1 cut(s) 39
HindII GTYRAC 1 cut(s) 39
HindIII AAGCTT 1 cut(s) 504
HinfI GANTC 1 cut(s) 317
HpaII CCGG 8 cut(s) 42, 95, 102, 164, 268, 284, 299, 409
Hpy166II GTNNAC 4 cut(s) 39, 169, 253, 481
Hpy188I TCNGA 5 cut(s) 11, 75, 322, 405, 476
Hpy188III TCNNGA 5 cut(s) 145, 181, 349, 368, 409
Hpy8I GTNNAC 4 cut(s) 39, 169, 253, 481
Hpy99I CGWCG 1 cut(s) 476
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 4 cut(s) 171, 249, 255, 471
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 104, 431
HpyF3I CTNAG 2 cut(s) 233, 507
HpySE526I ACGT 4 cut(s) 171, 249, 255, 471
Hsp92I GRCGYC 1 cut(s) 471
Hsp92II CATG 2 cut(s) 148, 365
HspAI GCGC 4 cut(s) 191, 203, 304, 359
Kpn2I TCCGGA 1 cut(s) 408
Kzo9I GATC 3 cut(s) 11, 400, 405
LmnI GCTCC 3 cut(s) 216, 295, 301
Lsp1109I GCAGC 3 cut(s) 85, 238, 267
MaeII ACGT 4 cut(s) 171, 249, 255, 471
MaeIII GTNAC 1 cut(s) 457
MalI GATC 3 cut(s) 13, 402, 407
MboI GATC 3 cut(s) 11, 400, 405
MboII GAAGA 3 cut(s) 88, 271, 274
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 2 cut(s) 213, 496
MluCI AATT 1 cut(s) 337
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 1 cut(s) 454
MnlI CCTC 6 cut(s) 79, 141, 228, 280, 307, 473
MroI TCCGGA 1 cut(s) 408
MseI TTAA 1 cut(s) 396
MspI CCGG 8 cut(s) 42, 95, 102, 164, 268, 284, 299, 409
MspR9I CCNGG 1 cut(s) 95
MvnI CGCG 2 cut(s) 65, 306
MwoI GCNNNNNNNGC 3 cut(s) 98, 104, 431
NciI CCSGG 1 cut(s) 95
NdeII GATC 3 cut(s) 11, 400, 405
NlaIII CATG 2 cut(s) 148, 365
NlaIV GGNNCC 4 cut(s) 106, 212, 297, 402
NmuCI GTSAC 1 cut(s) 457
NspI RCATGY 1 cut(s) 365
NspV TTCGAA 1 cut(s) 449
PagI TCATGA 1 cut(s) 144
PkrI GCNGC 3 cut(s) 100, 228, 282
PleI GAGTC 1 cut(s) 325
PpsI GAGTC 1 cut(s) 325
Ppu21I YACGTR 1 cut(s) 256
PspFI CCCAGC 1 cut(s) 205
PspN4I GGNNCC 4 cut(s) 106, 212, 297, 402
PstNI CAGNNNCTG 1 cut(s) 232
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 1 cut(s) 239
RsaNI GTAC 1 cut(s) 238
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 1 cut(s) 396
SatI GCNGC 3 cut(s) 99, 227, 281
Sau3AI GATC 3 cut(s) 11, 400, 405
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 1 cut(s) 95
SduI GDGCHC 2 cut(s) 213, 496
SfuI TTCGAA 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
Sse9I AATT 1 cut(s) 337
SsiI CCGC 3 cut(s) 63, 219, 440
StyD4I CCNGG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 4 cut(s) 174, 252, 258, 474
TaqI TCGA 2 cut(s) 38, 449
TasI AATT 1 cut(s) 337
Tru1I TTAA 1 cut(s) 396
Tru9I TTAA 1 cut(s) 396
TscAI CASTG 1 cut(s) 29
TseFI GTSAC 1 cut(s) 457
TseI GCWGC 3 cut(s) 98, 226, 280
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 4 cut(s) 32, 95, 133, 319
TspGWI ACGGA 1 cut(s) 479
TspRI CASTG 1 cut(s) 29
XapI RAATTY 1 cut(s) 337
XceI RCATGY 1 cut(s) 365
XmiI GTMKAC 4 cut(s) 38, 168, 252, 480
ZraI GACGTC 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.