MD14G1182500.v1.1
ERF Family

Histidine phosphatase superfamily (branch 1)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012122
Physical Location & Seq
Reverse (-)
27497096 .. 27499385
2290 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1182500.v1.1.491

Sequence Viewer

Length: 909 bp
ATGCTAGCAACATCTTCCTCCATTAAACCCCGCCCTCCTCCTCCTCCTCAATATCCATCGTCAACAACTCAAAACGCCATCAATTCATTCCTACCCATCAGCCGTCGGAGCCTCCTCGCCACGCTCACCGTCTCCACCGCCGCCGCAACACCTTTTGCACACAATTTTCTCAGCCTCATGTCAGCACATGAAGCAGCCTTTGCTCGTGGCCTCTTCCAGATGCCCCCAGTCAGGCTCACCAACCGGTATTTTCTGGTGAGGGCAGGGGAGTCTGAGTATGAGAGCATAGGAGTAATCAACACGAATCCGGTTGCAAAAACTTCTGTCGATAACGGATTGTCGGAGAAAGGGAAGAAGCAGGCAGTGAGGTCTGCTTTCGATTTGAAGGAAATGGGGGCTTGTGACAAGAACTGTTGGATATGGCCTTCTATCACTCAGAGAGCTTACCAGGCCGCAGAGATCATTGCAGCCGTTAATGGGGTCAGTCGAAGTTATATCGTCCCAGAGTATAGCTTTCTCGACGCCCGTGGATTGGGGGCTTATGAAGGAAAGAACCTAGATGCTGTTTCAGAAGTATATGCATCAGACACTCTTTCTCCCGCAATCAAGCCTCCCCCCATCGATGATGGAACCCCGAACGAGAGTGTCTCAGATGTATTCGTTCGGGTGACACAGCTCATGTCCATACTCGAGACTCAGTACTCCGAAGACACTGTGATCATTGTCTCACCAGATTCAGACAATTTGACTGTCCTGCAAGCTGGTATAATTGGACTTGACTTGCGCAGGCACAGGGAACTTTCTTTTGCACCAGGAGAAGTCAGATTTGTTGATACCAATAGTGTACCTACTTACAAGCAACCTGCATCAGCTGTGTATAAATGCCCAAAACCACCAAATTGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
His_Phos_1

Protein Analysis

303

Amino Acids

32.83

Weight (kDa)

6.33

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
His_Phos_1 PF00300 84 - 277 1.5e-13 Histidine phosphatase superfamily (branch 1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012122)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G62840 AT5G62840
fragaria_vesca FvH4_5g05200 FvH4_5g05200 FvH4_5g05200
malus_domestica MD14G1182500.v1.1
prunus_persica Prupe.5G175000_v2.0.a1 Prupe.5G175000_v2.0.a1 Prupe.5G175000_v2.0.a1
pyrus_communis pycom14g15160
rosa_chinensis RchiOBHm_Chr7g0193711
rosa_laevigata RLG00000004249
rosa_roxburghii Rroxscaffold_3G00261880
rosa_rugosa Rorug07G0011400
rosa_samantha Rh7AG138300 Rh7BG138500 Rh7CG142100 Rh7DG139600
rosa_wichuraiana Rw7G011780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 785
Acc36I ACCTGC 1 cut(s) 871
AciI CCGC 6 cut(s) 31, 138, 141, 144, 453, 600
AcyI GRCGYC 1 cut(s) 522
AfaI GTAC 2 cut(s) 701, 846
AfiI CCNNNNNNNGG 3 cut(s) 231, 477, 532
AgeI ACCGGT 1 cut(s) 243
AgsI TTSAA 1 cut(s) 385
AjnI CCWGG 2 cut(s) 447, 811
AluBI AGCT 5 cut(s) 443, 513, 676, 761, 872
AluI AGCT 5 cut(s) 443, 513, 676, 761, 872
Alw26I GTCTC 4 cut(s) 136, 652, 686, 730
Ama87I CYCGRG 1 cut(s) 689
AoxI GGCC 3 cut(s) 208, 422, 450
ApeKI GCWGC 2 cut(s) 194, 467
AsiGI ACCGGT 1 cut(s) 243
AspLEI GCGC 1 cut(s) 786
AsuHPI GGTGA 5 cut(s) 118, 229, 268, 679, 720
AsuNHI GCTAGC 1 cut(s) 4
AvaI CYCGRG 1 cut(s) 689
BauI CACGAG 1 cut(s) 204
BbsI GAAGAC 1 cut(s) 714
BbvI GCAGC 2 cut(s) 206, 479
BccI CCATC 5 cut(s) 64, 86, 104, 620, 626
BceAI ACGGC 2 cut(s) 87, 455
BciT130I CCWGG 2 cut(s) 449, 813
BclI TGATCA 1 cut(s) 717
BcoDI GTCTC 4 cut(s) 136, 652, 686, 730
BfaI CTAG 2 cut(s) 5, 557
BfuAI ACCTGC 1 cut(s) 871
BisI GCNGC 5 cut(s) 141, 144, 195, 453, 468
BlsI GCNGC 5 cut(s) 142, 145, 196, 454, 469
BmcAI AGTACT 1 cut(s) 701
Bme1390I CCNGG 2 cut(s) 449, 813
BmeT110I CYCGRG 1 cut(s) 689
BmiI GGNNCC 2 cut(s) 110, 631
BmrFI CCNGG 2 cut(s) 449, 813
BmrI ACTGGG 1 cut(s) 221
BmsI GCATC 4 cut(s) 210, 550, 590, 875
BmtI GCTAGC 1 cut(s) 8
BmuI ACTGGG 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 714
BplI GAGNNNNNCTC 2 cut(s) 632, 664
Bsa29I ATCGAT 1 cut(s) 621
BsaHI GRCGYC 1 cut(s) 522
BsaJI CCNNGG 1 cut(s) 526
BsaWI WCCGGW 2 cut(s) 243, 307
BsaXI ACNNNNNCTCC 4 cut(s) 260, 290, 580, 610
Bsc4I CCNNNNNNNGG 3 cut(s) 231, 477, 532
Bse118I RCCGGY 1 cut(s) 243
Bse1I ACTGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 462
BseBI CCWGG 2 cut(s) 449, 813
BseCI ATCGAT 1 cut(s) 621
BseDI CCNNGG 1 cut(s) 526
BseLI CCNNNNNNNGG 3 cut(s) 231, 477, 532
BseMI GCAATG 1 cut(s) 462
BseMII CTCAG 5 cut(s) 184, 264, 449, 663, 710
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 5 cut(s) 27, 30, 33, 36, 104
BseXI GCAGC 2 cut(s) 206, 479
BshFI GGCC 3 cut(s) 210, 424, 452
BshTI ACCGGT 1 cut(s) 243
BshVI ATCGAT 1 cut(s) 621
BsiHKCI CYCGRG 1 cut(s) 689
BsiSI CCGG 2 cut(s) 244, 308
BslFI GGGAC 1 cut(s) 485
BslI CCNNNNNNNGG 3 cut(s) 231, 477, 532
BsmAI GTCTC 4 cut(s) 136, 652, 686, 730
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 1 cut(s) 485
BsnI GGCC 3 cut(s) 210, 424, 452
BsoBI CYCGRG 1 cut(s) 689
Bsp143I GATC 2 cut(s) 459, 717
BspACI CCGC 6 cut(s) 31, 138, 141, 144, 453, 600
BspANI GGCC 3 cut(s) 210, 424, 452
BspCNI CTCAG 5 cut(s) 183, 265, 448, 662, 709
BspDI ATCGAT 1 cut(s) 621
BspLI GGNNCC 2 cut(s) 110, 631
BspMI ACCTGC 1 cut(s) 871
BspOI GCTAGC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 243
BsrI ACTGG 1 cut(s) 227
BssAI RCCGGY 1 cut(s) 243
BssECI CCNNGG 1 cut(s) 526
BssMI GATC 2 cut(s) 459, 717
BssNI GRCGYC 1 cut(s) 522
BssSI CACGAG 1 cut(s) 204
Bst2BI CACGAG 1 cut(s) 204
Bst2UI CCWGG 2 cut(s) 449, 813
Bst4CI ACNGT 4 cut(s) 130, 413, 715, 751
Bst6I CTCTTC 1 cut(s) 218
BstACI GRCGYC 1 cut(s) 522
BstAPI GCANNNNNTGC 1 cut(s) 200
BstC8I GCNNGC 4 cut(s) 6, 360, 759, 788
BstDEI CTNAG 5 cut(s) 170, 273, 435, 649, 696
BstDSI CCRYGG 1 cut(s) 526
BstHHI GCGC 1 cut(s) 786
BstKTI GATC 2 cut(s) 462, 720
BstMAI GTCTC 4 cut(s) 136, 652, 686, 730
BstMBI GATC 2 cut(s) 459, 717
BstMWI GCNNNNNNNGC 4 cut(s) 108, 191, 200, 449
BstNI CCWGG 2 cut(s) 449, 813
BstSCI CCNGG 2 cut(s) 447, 811
BstV1I GCAGC 2 cut(s) 206, 479
BstV2I GAAGAC 1 cut(s) 714
Bsu15I ATCGAT 1 cut(s) 621
BsuRI GGCC 3 cut(s) 210, 424, 452
BsuTUI ATCGAT 1 cut(s) 621
BtgI CCRYGG 1 cut(s) 526
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 369, 711
BveI ACCTGC 1 cut(s) 871
Cac8I GCNNGC 4 cut(s) 6, 360, 759, 788
CfoI GCGC 1 cut(s) 786
Cfr10I RCCGGY 1 cut(s) 243
ClaI ATCGAT 1 cut(s) 621
CseI GACGC 1 cut(s) 530
Csp6I GTAC 2 cut(s) 700, 845
CspAI ACCGGT 1 cut(s) 243
CviAII CATG 3 cut(s) 178, 188, 679
CviQI GTAC 2 cut(s) 700, 845
DdeI CTNAG 5 cut(s) 170, 273, 435, 649, 696
DpnI GATC 2 cut(s) 461, 719
DpnII GATC 2 cut(s) 459, 717
Eam1104I CTCTTC 1 cut(s) 218
EarI CTCTTC 1 cut(s) 218
Eco88I CYCGRG 1 cut(s) 689
EcoRII CCWGG 2 cut(s) 447, 811
EcoT22I ATGCAT 1 cut(s) 583
Esp3I CGTCTC 1 cut(s) 136
FaeI CATG 3 cut(s) 181, 191, 682
FaqI GGGAC 1 cut(s) 485
FatI CATG 3 cut(s) 177, 187, 678
FauI CCCGC 2 cut(s) 38, 607
FbaI TGATCA 1 cut(s) 717
Fnu4HI GCNGC 5 cut(s) 141, 144, 195, 453, 468
Fsp4HI GCNGC 5 cut(s) 141, 144, 195, 453, 468
FspBI CTAG 2 cut(s) 5, 557
FspI TGCGCA 1 cut(s) 785
GlaI GCGC 1 cut(s) 785
GluI GCNGC 5 cut(s) 141, 144, 195, 453, 468
HaeIII GGCC 3 cut(s) 210, 424, 452
HapII CCGG 2 cut(s) 244, 308
HgaI GACGC 1 cut(s) 530
HhaI GCGC 1 cut(s) 786
Hin1I GRCGYC 1 cut(s) 522
Hin1II CATG 3 cut(s) 181, 191, 682
Hin6I GCGC 1 cut(s) 784
HinP1I GCGC 1 cut(s) 784
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 4 cut(s) 269, 304, 694, 734
HpaII CCGG 2 cut(s) 244, 308
HphI GGTGA 5 cut(s) 118, 229, 268, 679, 720
Hpy166II GTNNAC 2 cut(s) 63, 845
Hpy188III TCNNGA 3 cut(s) 217, 518, 691
Hpy8I GTNNAC 2 cut(s) 63, 845
Hpy99I CGWCG 2 cut(s) 108, 524
HpyAV CCTTC 3 cut(s) 379, 435, 539
HpyCH4III ACNGT 4 cut(s) 130, 413, 715, 751
HpyCH4V TGCA 7 cut(s) 158, 314, 467, 581, 757, 809, 866
HpyF10VI GCNNNNNNNGC 4 cut(s) 108, 191, 200, 449
HpyF3I CTNAG 5 cut(s) 170, 273, 435, 649, 696
Hsp92I GRCGYC 1 cut(s) 522
Hsp92II CATG 3 cut(s) 181, 191, 682
HspAI GCGC 1 cut(s) 784
Ksp22I TGATCA 1 cut(s) 717
Kzo9I GATC 2 cut(s) 459, 717
LmnI GCTCC 1 cut(s) 108
Lsp1109I GCAGC 2 cut(s) 206, 479
LweI GCATC 4 cut(s) 210, 550, 590, 875
MaeI CTAG 2 cut(s) 5, 557
MaeIII GTNAC 3 cut(s) 401, 667, 902
MalI GATC 2 cut(s) 461, 719
MboI GATC 2 cut(s) 459, 717
MboII GAAGA 4 cut(s) 6, 205, 364, 719
MluCI AATT 5 cut(s) 82, 163, 742, 768, 898
MlyI GAGTC 2 cut(s) 278, 688
MmeI TCCRAC 3 cut(s) 86, 321, 395
Mph1103I ATGCAT 1 cut(s) 583
MseI TTAA 2 cut(s) 24, 474
MspA1I CMGCKG 1 cut(s) 872
MspI CCGG 2 cut(s) 244, 308
MspR9I CCNGG 2 cut(s) 449, 813
MvaI CCWGG 2 cut(s) 449, 813
MwoI GCNNNNNNNGC 4 cut(s) 108, 191, 200, 449
NdeII GATC 2 cut(s) 459, 717
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 3 cut(s) 181, 191, 682
NlaIV GGNNCC 2 cut(s) 110, 631
NmuCI GTSAC 2 cut(s) 401, 667
NsbI TGCGCA 1 cut(s) 785
NsiI ATGCAT 1 cut(s) 583
PaeR7I CTCGAG 1 cut(s) 689
PfeI GAWTC 2 cut(s) 304, 734
PinAI ACCGGT 1 cut(s) 243
PkrI GCNGC 5 cut(s) 142, 145, 196, 454, 469
PleI GAGTC 2 cut(s) 277, 688
PpsI GAGTC 2 cut(s) 277, 688
Psp6I CCWGG 2 cut(s) 447, 811
PspGI CCWGG 2 cut(s) 447, 811
PspN4I GGNNCC 2 cut(s) 110, 631
PvuII CAGCTG 1 cut(s) 872
RsaI GTAC 2 cut(s) 701, 846
RsaNI GTAC 2 cut(s) 700, 845
SaqAI TTAA 2 cut(s) 24, 474
SatI GCNGC 5 cut(s) 141, 144, 195, 453, 468
Sau3AI GATC 2 cut(s) 459, 717
ScaI AGTACT 1 cut(s) 701
SchI GAGTC 2 cut(s) 278, 688
ScrFI CCNGG 2 cut(s) 449, 813
SfaNI GCATC 4 cut(s) 210, 550, 590, 875
Sfr274I CTCGAG 1 cut(s) 689
SlaI CTCGAG 1 cut(s) 689
SmlI CTYRAG 1 cut(s) 689
SmoI CTYRAG 1 cut(s) 689
Sse9I AATT 5 cut(s) 82, 163, 742, 768, 898
SsiI CCGC 6 cut(s) 31, 138, 141, 144, 453, 600
SspMI CTAG 2 cut(s) 5, 557
StyD4I CCNGG 2 cut(s) 447, 811
TaaI ACNGT 4 cut(s) 130, 413, 715, 751
TaqI TCGA 6 cut(s) 327, 378, 487, 519, 621, 690
TasI AATT 5 cut(s) 82, 163, 742, 768, 898
TatI WGTACW 1 cut(s) 699
TauI GCSGC 3 cut(s) 143, 146, 455
TfiI GAWTC 2 cut(s) 304, 734
Tru1I TTAA 2 cut(s) 24, 474
Tru9I TTAA 2 cut(s) 24, 474
TscAI CASTG 2 cut(s) 369, 718
TseFI GTSAC 2 cut(s) 401, 667
TseI GCWGC 2 cut(s) 194, 467
Tsp45I GTSAC 2 cut(s) 401, 667
TspDTI ATGAA 3 cut(s) 75, 204, 558
TspGWI ACGGA 1 cut(s) 348
TspRI CASTG 2 cut(s) 369, 718
XhoI CTCGAG 1 cut(s) 689
XspI CTAG 2 cut(s) 5, 557
ZrmI AGTACT 1 cut(s) 701
Zsp2I ATGCAT 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.