MD14G1209100.v1.1
MYB Family

SANT SWI3, ADA2, N-CoR and TFIIIB'' DNA-binding domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0013675
Physical Location & Seq
Reverse (-)
29540267 .. 29544030
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1209100.v1.1.491

Sequence Viewer

Length: 1545 bp
ATGTTTAATATGGTGCAGAAACGATCACTTGATGATGAGGAAATATTTGTTTCTTTCAAGCACCCAAGACAAGTGGGACATAATAAAGAGCTAGTTTCTTTCTCAGAATCTGTTTTTCCTGGAGATGTTTCTGAGGAACCAGAGACTTTAGAGAATGGATGTGCAAAAGACAATAATAGAAGTGAAGAGGAGATTTCTGATGACAAATTTTCTGGTCTTCCAAAAGGCGGTGAGGATGTTGAAACCAGCGCACCTAGAAGCTTTACCATATCCTCATGGACCACTAGCAGCAATAGTGGAGAAGATTCTCTGTTTGAAGCATCATTTCATGGGTCCTTTTTCCCTGAGTACTTCAATCATGAACGTTCAATAAGGACCTTGACTCAATTTGAGGATATTTATTCTGTTCTTTTGGATCACCCTCCTCGGAAGTCTGCCTCCATTGGACCAGAGCATCAAGCTGATGTTCCACTGTGGGGTGCACAAGGAGCCCATAATAATTTTGACCTTGAGGATGAGAAACGGCTAATGGGGACTTGTGTAATTCCAATGCCTGATTCTGATACTGAGTTATTTACTGACACGGGTTGCATAGTTGGAAAGGGCAGAATTGATTGCAGCTGTGAAGATGGAGAGTCTGTTAGATGTGTCAGGCAGCACATTTTGGAAGCAAGAGAGAAACTTGTAAAGACCATCGGGCCAAAGAGATTTGAGGAGCTAGGATTTAATGACATGGGAGAGCAAGTGGCACAAACTTGGAGTGAAGAAGAGGAACAACTATTCCACCGGGTTGTCTTCTCAAATCCAACATCTTTGGGCAAGAATTTCTGGGACAAACTTTCAACTGTCTTCCCCTCCAGGACGAAAAAGGAGATTGTCAGTTACTATTTCAATGTCTTTATGCTTAGGAAGAGGGCAGAGCAGAACAGGTATGACCCAATGAATATTGATAGTGACAGTGATGAATGGCAGGGGAGTAATGATTACGGTGAAAATCAACCCTCTGTGACAGAGGATGAGGACTCGGTGGTTGAGTCTCCTGAAGGTAGACGTGTCCCTGGTTATAAGCGAAATTGGAAGGATGATTTGGAAGTGCTCGATGAGGACGATACGGATGAAACTTGTGATGAAAATGTTGATGTGGATATGTTTGGGCGGGGACCTGAGCAAATTTTAGATAGATGCTACGGGTTGGTTGACAACTGCAGTTCCTGTCCCATAGCTCAGCTTCAGGATAAGATTTCCTGGGATGAAAAGGGAGATCAAGAAGTCCAAGATGATTCATGCACGTCCTTTGATGCAGCCGTGGCTGCTTCGCAAGAGAGTCAGCGGAAGTCTGAGAACGTGAATCATTGGTCGGGTAGTTTTAACCGCGGTGACCACGAATTTGTCTTGGAGCCTTGTGATGCCAAAATCTGGGATGCTGGGTATATGACTTGCCCTAAAAACAAGGTTGACTTCTTGCCAACTAGCAACATGATTGAAGAGGTTTTCGGGAAAGAATCATGGAATCACAAAGCAAGGGATGGCAAAAACTTGGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

515

Amino Acids

58.41

Weight (kDa)

4.51

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 252 - 299 2.8e-06 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1065
AccB7I CCANNNNNTGG 1 cut(s) 1416
AccI GTMKAC 1 cut(s) 1048
AccII CGCG 1 cut(s) 1374
AciI CCGC 5 cut(s) 228, 1156, 1330, 1372, 1374
AclI AACGTT 1 cut(s) 364
AclWI GGATC 1 cut(s) 423
AcsI RAATTY 4 cut(s) 206, 823, 1170, 1385
AcuI CTGAAG 2 cut(s) 1062, 1214
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 3 cut(s) 227, 476, 1416
AflIII ACRYGT 1 cut(s) 1051
AgsI TTSAA 8 cut(s) 58, 242, 317, 355, 369, 843, 892, 1484
AjiI CACGTC 2 cut(s) 1052, 1290
AjnI CCWGG 4 cut(s) 118, 857, 1057, 1244
AjuI GAANNNNNNNTTGG 4 cut(s) 541, 573, 1070, 1102
AloI GAACNNNNNNTCC 2 cut(s) 765, 797
AluBI AGCT 7 cut(s) 91, 261, 461, 621, 718, 1223, 1228
AluI AGCT 7 cut(s) 91, 261, 461, 621, 718, 1223, 1228
Alw21I GWGCWC 2 cut(s) 484, 1098
Alw26I GTCTC 2 cut(s) 137, 1041
Alw44I GTGCAC 1 cut(s) 480
AlwI GGATC 1 cut(s) 423
AlwNI CAGNNNCTG 2 cut(s) 110, 1212
AoxI GGCC 1 cut(s) 698
ApaLI GTGCAC 1 cut(s) 480
ApeKI GCWGC 5 cut(s) 288, 618, 655, 1301, 1310
ApoI RAATTY 4 cut(s) 206, 823, 1170, 1385
ArsI GACNNNNNNTTYG 2 cut(s) 1370, 1402
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 6 cut(s) 279, 333, 375, 446, 698, 1160
AsuC2I CCSGG 1 cut(s) 788
AsuHPI GGTGA 4 cut(s) 242, 410, 1001, 1388
AvaII GGWCC 5 cut(s) 279, 333, 375, 446, 1160
BaeGI GKGCMC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 493
BbsI GAAGAC 3 cut(s) 209, 787, 841
Bbv12I GWGCWC 2 cut(s) 484, 1098
BbvI GCAGC 5 cut(s) 300, 630, 667, 1297, 1313
BccI CCATC 3 cut(s) 623, 701, 1520
BceAI ACGGC 2 cut(s) 539, 1289
BciT130I CCWGG 4 cut(s) 120, 859, 1059, 1246
BcnI CCSGG 1 cut(s) 788
BcoDI GTCTC 2 cut(s) 137, 1041
BfaI CTAG 6 cut(s) 92, 255, 285, 719, 1470, 1543
BfmI CTRYAG 1 cut(s) 1204
BisI GCNGC 5 cut(s) 289, 619, 656, 1302, 1311
BlpI GCTNAGC 1 cut(s) 1224
BlsI GCNGC 5 cut(s) 290, 620, 657, 1303, 1312
BmcAI AGTACT 1 cut(s) 350
Bme1390I CCNGG 5 cut(s) 120, 788, 859, 1059, 1246
Bme18I GGWCC 5 cut(s) 279, 333, 375, 446, 1160
BmgBI CACGTC 2 cut(s) 1052, 1290
BmgT120I GGNCC 6 cut(s) 279, 333, 375, 446, 698, 1160
BmiI GGNNCC 5 cut(s) 138, 334, 490, 1161, 1398
BmrFI CCNGG 5 cut(s) 120, 788, 859, 1059, 1246
BmsI GCATC 6 cut(s) 329, 463, 1172, 1288, 1396, 1411
BpiI GAAGAC 3 cut(s) 209, 787, 841
BpmI CTGGAG 2 cut(s) 141, 841
Bpu10I CCTNAGC 2 cut(s) 905, 1164
Bpu1102I GCTNAGC 1 cut(s) 1224
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 788
BsaJI CCNNGG 5 cut(s) 425, 1057, 1245, 1305, 1372
BsaXI ACNNNNNCTCC 4 cut(s) 624, 654, 729, 759
Bsc4I CCNNNNNNNGG 3 cut(s) 227, 476, 1416
BseBI CCWGG 4 cut(s) 120, 859, 1059, 1246
BseDI CCNNGG 5 cut(s) 425, 1057, 1245, 1305, 1372
BseGI GGATG 9 cut(s) 164, 241, 520, 1021, 1087, 1120, 1255, 1426, 1531
BseLI CCNNNNNNNGG 3 cut(s) 227, 476, 1416
BseMII CTCAG 7 cut(s) 117, 123, 336, 558, 1155, 1238, 1329
BseRI GAGGAG 3 cut(s) 203, 414, 728
BseSI GKGCMC 1 cut(s) 484
BseXI GCAGC 5 cut(s) 300, 630, 667, 1297, 1313
BseYI CCCAGC 1 cut(s) 1424
BsgI GTGCAG 1 cut(s) 35
Bsh1236I CGCG 1 cut(s) 1374
BshFI GGCC 1 cut(s) 700
BsiHKAI GWGCWC 2 cut(s) 484, 1098
BsiSI CCGG 1 cut(s) 787
BslFI GGGAC 6 cut(s) 90, 547, 845, 1040, 1173, 1200
BslI CCNNNNNNNGG 3 cut(s) 227, 476, 1416
BsmAI GTCTC 2 cut(s) 137, 1041
BsmFI GGGAC 6 cut(s) 90, 547, 845, 1040, 1173, 1200
BsnI GGCC 1 cut(s) 700
Bsp1286I GDGCHC 3 cut(s) 484, 493, 1098
Bsp143I GATC 3 cut(s) 23, 415, 1261
Bsp1720I GCTNAGC 1 cut(s) 1224
BspACI CCGC 5 cut(s) 228, 1156, 1330, 1372, 1374
BspANI GGCC 1 cut(s) 700
BspCNI CTCAG 7 cut(s) 116, 124, 337, 559, 1156, 1237, 1330
BspFNI CGCG 1 cut(s) 1374
BspHI TCATGA 1 cut(s) 358
BspLI GGNNCC 5 cut(s) 138, 334, 490, 1161, 1398
BspMAI CTGCAG 1 cut(s) 1208
BspPI GGATC 1 cut(s) 423
BssECI CCNNGG 5 cut(s) 425, 1057, 1245, 1305, 1372
BssMI GATC 3 cut(s) 23, 415, 1261
Bst2UI CCWGG 4 cut(s) 120, 859, 1059, 1246
Bst4CI ACNGT 4 cut(s) 474, 847, 959, 989
Bst6I CTCTTC 4 cut(s) 180, 762, 905, 1479
BstDEI CTNAG 8 cut(s) 103, 132, 345, 567, 905, 1164, 1224, 1338
BstDSI CCRYGG 2 cut(s) 1305, 1372
BstEII GGTNACC 1 cut(s) 1376
BstF5I GGATG 9 cut(s) 164, 241, 520, 1021, 1087, 1120, 1255, 1426, 1531
BstFNI CGCG 1 cut(s) 1374
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 3 cut(s) 26, 418, 1264
BstMAI GTCTC 2 cut(s) 137, 1041
BstMBI GATC 3 cut(s) 23, 415, 1261
BstMWI GCNNNNNNNGC 3 cut(s) 488, 1307, 1310
BstNI CCWGG 4 cut(s) 120, 859, 1059, 1246
BstPI GGTNACC 1 cut(s) 1376
BstSCI CCNGG 5 cut(s) 118, 786, 857, 1057, 1244
BstSFI CTRYAG 1 cut(s) 1204
BstSLI GKGCMC 1 cut(s) 484
BstUI CGCG 1 cut(s) 1374
BstV1I GCAGC 5 cut(s) 300, 630, 667, 1297, 1313
BstV2I GAAGAC 3 cut(s) 209, 787, 841
BsuRI GGCC 1 cut(s) 700
BtgI CCRYGG 2 cut(s) 1305, 1372
BtrI CACGTC 2 cut(s) 1052, 1290
BtsCI GGATG 9 cut(s) 164, 241, 520, 1021, 1087, 1120, 1255, 1426, 1531
BtsIMutI CAGTG 2 cut(s) 470, 964
CaiI CAGNNNCTG 2 cut(s) 110, 1212
CciI TCATGA 1 cut(s) 358
CfoI GCGC 1 cut(s) 251
Cfr13I GGNCC 6 cut(s) 279, 333, 375, 446, 698, 1160
Cfr42I CCGCGG 1 cut(s) 1375
Csp6I GTAC 1 cut(s) 349
CspCI CAANNNNNGTGG 2 cut(s) 54, 89
CviAII CATG 7 cut(s) 276, 329, 359, 733, 1284, 1477, 1506
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 8 cut(s) 103, 132, 345, 567, 905, 1164, 1224, 1338
DpnI GATC 3 cut(s) 25, 417, 1263
DpnII GATC 3 cut(s) 23, 415, 1261
Eam1104I CTCTTC 4 cut(s) 180, 762, 905, 1479
EarI CTCTTC 4 cut(s) 180, 762, 905, 1479
Eco24I GRGCYC 1 cut(s) 493
Eco47I GGWCC 5 cut(s) 279, 333, 375, 446, 1160
Eco57I CTGAAG 2 cut(s) 1062, 1214
Eco91I GGTNACC 1 cut(s) 1376
EcoO109I RGGNCCY 3 cut(s) 333, 375, 1160
EcoO65I GGTNACC 1 cut(s) 1376
EcoRII CCWGG 4 cut(s) 118, 857, 1057, 1244
EcoT38I GRGCYC 1 cut(s) 493
FaeI CATG 7 cut(s) 279, 332, 362, 736, 1287, 1480, 1509
FalI AAGNNNNNCTT 2 cut(s) 1442, 1474
FaqI GGGAC 6 cut(s) 90, 547, 845, 1040, 1173, 1200
FatI CATG 7 cut(s) 275, 328, 358, 732, 1283, 1476, 1505
FauI CCCGC 1 cut(s) 1149
FblI GTMKAC 1 cut(s) 1048
Fnu4HI GCNGC 5 cut(s) 289, 619, 656, 1302, 1311
FokI GGATG 9 cut(s) 171, 248, 527, 1028, 1094, 1127, 1262, 1433, 1538
FriOI GRGCYC 1 cut(s) 493
Fsp4HI GCNGC 5 cut(s) 289, 619, 656, 1302, 1311
FspBI CTAG 6 cut(s) 92, 255, 285, 719, 1470, 1543
GlaI GCGC 1 cut(s) 250
GluI GCNGC 5 cut(s) 289, 619, 656, 1302, 1311
GsaI CCCAGC 1 cut(s) 1428
GsuI CTGGAG 2 cut(s) 141, 841
HaeIII GGCC 1 cut(s) 700
HapII CCGG 1 cut(s) 787
HhaI GCGC 1 cut(s) 251
Hin1II CATG 7 cut(s) 279, 332, 362, 736, 1287, 1480, 1509
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 2 cut(s) 1198, 1456
HindII GTYRAC 2 cut(s) 1198, 1456
HindIII AAGCTT 1 cut(s) 259
HpaII CCGG 1 cut(s) 787
HphI GGTGA 4 cut(s) 242, 410, 1001, 1388
Hpy166II GTNNAC 4 cut(s) 482, 1049, 1198, 1456
Hpy188I TCNGA 6 cut(s) 106, 133, 199, 429, 562, 1339
Hpy188III TCNNGA 5 cut(s) 359, 1040, 1232, 1265, 1495
Hpy8I GTNNAC 4 cut(s) 482, 1049, 1198, 1456
HpyAV CCTTC 2 cut(s) 1037, 1072
HpyCH4III ACNGT 4 cut(s) 474, 847, 959, 989
HpyCH4IV ACGT 4 cut(s) 364, 1051, 1289, 1344
HpyCH4V TGCA 8 cut(s) 16, 164, 482, 591, 618, 1206, 1287, 1301
HpyF10VI GCNNNNNNNGC 3 cut(s) 488, 1307, 1310
HpyF3I CTNAG 8 cut(s) 103, 132, 345, 567, 905, 1164, 1224, 1338
HpySE526I ACGT 4 cut(s) 364, 1051, 1289, 1344
Hsp92II CATG 7 cut(s) 279, 332, 362, 736, 1287, 1480, 1509
HspAI GCGC 1 cut(s) 249
KspI CCGCGG 1 cut(s) 1375
Kzo9I GATC 3 cut(s) 23, 415, 1261
LmnI GCTCC 3 cut(s) 488, 715, 1396
Lsp1109I GCAGC 5 cut(s) 300, 630, 667, 1297, 1313
LweI GCATC 6 cut(s) 329, 463, 1172, 1288, 1396, 1411
MaeI CTAG 6 cut(s) 92, 255, 285, 719, 1470, 1543
MaeII ACGT 4 cut(s) 364, 1051, 1289, 1344
MaeIII GTNAC 4 cut(s) 881, 953, 1006, 1376
MalI GATC 3 cut(s) 25, 417, 1263
MboI GATC 3 cut(s) 23, 415, 1261
MhlI GDGCHC 3 cut(s) 484, 493, 1098
MluCI AATT 9 cut(s) 206, 386, 499, 543, 609, 823, 1072, 1170, 1385
MlyI GAGTC 5 cut(s) 376, 644, 1016, 1043, 1333
MmeI TCCRAC 2 cut(s) 577, 830
MseI TTAA 3 cut(s) 6, 726, 1368
MspA1I CMGCKG 3 cut(s) 621, 1330, 1374
MspI CCGG 1 cut(s) 787
MspR9I CCNGG 5 cut(s) 120, 788, 859, 1059, 1246
MvaI CCWGG 4 cut(s) 120, 859, 1059, 1246
MvnI CGCG 1 cut(s) 1374
MwoI GCNNNNNNNGC 3 cut(s) 488, 1307, 1310
NciI CCSGG 1 cut(s) 788
NdeII GATC 3 cut(s) 23, 415, 1261
NlaIII CATG 7 cut(s) 279, 332, 362, 736, 1287, 1480, 1509
NlaIV GGNNCC 5 cut(s) 138, 334, 490, 1161, 1398
NmuCI GTSAC 3 cut(s) 953, 1006, 1376
PagI TCATGA 1 cut(s) 358
PcsI WCGNNNNNNNCGW 1 cut(s) 1104
PfeI GAWTC 7 cut(s) 107, 305, 557, 1280, 1348, 1502, 1510
PflMI CCANNNNNTGG 1 cut(s) 1416
PfoI TCCNGGA 2 cut(s) 118, 857
PkrI GCNGC 5 cut(s) 290, 620, 657, 1303, 1312
PleI GAGTC 5 cut(s) 376, 643, 1016, 1042, 1332
PpsI GAGTC 5 cut(s) 376, 643, 1016, 1042, 1332
PpuMI RGGWCCY 3 cut(s) 333, 375, 1160
PsiI TTATAA 1 cut(s) 1065
Psp1406I AACGTT 1 cut(s) 364
Psp5II RGGWCCY 3 cut(s) 333, 375, 1160
Psp6I CCWGG 4 cut(s) 118, 857, 1057, 1244
PspEI GGTNACC 1 cut(s) 1376
PspFI CCCAGC 1 cut(s) 1424
PspGI CCWGG 4 cut(s) 118, 857, 1057, 1244
PspN4I GGNNCC 5 cut(s) 138, 334, 490, 1161, 1398
PspPI GGNCC 6 cut(s) 279, 333, 375, 446, 698, 1160
PspPPI RGGWCCY 3 cut(s) 333, 375, 1160
PstI CTGCAG 1 cut(s) 1208
PstNI CAGNNNCTG 2 cut(s) 110, 1212
PvuII CAGCTG 1 cut(s) 621
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SacII CCGCGG 1 cut(s) 1375
SaqAI TTAA 3 cut(s) 6, 726, 1368
SatI GCNGC 5 cut(s) 289, 619, 656, 1302, 1311
Sau3AI GATC 3 cut(s) 23, 415, 1261
Sau96I GGNCC 6 cut(s) 279, 333, 375, 446, 698, 1160
ScaI AGTACT 1 cut(s) 350
SchI GAGTC 5 cut(s) 376, 644, 1016, 1043, 1333
ScrFI CCNGG 5 cut(s) 120, 788, 859, 1059, 1246
SduI GDGCHC 3 cut(s) 484, 493, 1098
SfaNI GCATC 6 cut(s) 329, 463, 1172, 1288, 1396, 1411
SfcI CTRYAG 1 cut(s) 1204
Sfr303I CCGCGG 1 cut(s) 1375
SgrBI CCGCGG 1 cut(s) 1375
SinI GGWCC 5 cut(s) 279, 333, 375, 446, 1160
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 9 cut(s) 206, 386, 499, 543, 609, 823, 1072, 1170, 1385
SsiI CCGC 5 cut(s) 228, 1156, 1330, 1372, 1374
SspI AATATT 2 cut(s) 45, 946
SspMI CTAG 6 cut(s) 92, 255, 285, 719, 1470, 1543
StyD4I CCNGG 5 cut(s) 118, 786, 857, 1057, 1244
TaaI ACNGT 4 cut(s) 474, 847, 959, 989
TaiI ACGT 4 cut(s) 367, 1054, 1292, 1347
TaqI TCGA 1 cut(s) 1098
TasI AATT 9 cut(s) 206, 386, 499, 543, 609, 823, 1072, 1170, 1385
TatI WGTACW 1 cut(s) 348
TfiI GAWTC 7 cut(s) 107, 305, 557, 1280, 1348, 1502, 1510
Tru1I TTAA 3 cut(s) 6, 726, 1368
Tru9I TTAA 3 cut(s) 6, 726, 1368
TscAI CASTG 2 cut(s) 477, 964
TseFI GTSAC 3 cut(s) 953, 1006, 1376
TseI GCWGC 5 cut(s) 288, 618, 655, 1301, 1310
Tsp45I GTSAC 3 cut(s) 953, 1006, 1376
TspDTI ATGAA 8 cut(s) 317, 375, 956, 978, 1131, 1143, 1266, 1272
TspGWI ACGGA 1 cut(s) 1127
TspRI CASTG 2 cut(s) 477, 964
Van91I CCANNNNNTGG 1 cut(s) 1416
VneI GTGCAC 1 cut(s) 480
VpaK11BI GGWCC 5 cut(s) 279, 333, 375, 446, 1160
XapI RAATTY 4 cut(s) 206, 823, 1170, 1385
XmiI GTMKAC 1 cut(s) 1048
XspI CTAG 6 cut(s) 92, 255, 285, 719, 1470, 1543
ZrmI AGTACT 1 cut(s) 350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.