MD14G1222200.v1.1
MYB Family

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0014455
Physical Location & Seq
Forward (+)
30387645 .. 30389123
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1222200.v1.1.491

Sequence Viewer

Length: 924 bp
ATGTCGAGTTCTAGCGTTTGGAGCAAAGAAGAAGACAAAGCGTTCGAGAACGCAATTGCCATGCATTGGATCGATGAAAATTCAGAAGAACAGTGGGAAAAGATTGCTGAGTTGGTTCCAAGCAAAAGCATGGAGGAGTTAAAGCAACACTACCAAATGCTAGTCGACGACGTGGGTGCAATCGAGGCGGGGCGAGTGTCCCCGCCTAACTACACAGCTGATGAAGCTGCTAATACAGTATCGTCCAGTAAGGACAGTGGCCATCGTGCTTCTTCTTCCGGGACTTCGGCTTCTGATAAGAGATTGAATTGCGGTCACGGAGGTGGGTTTTTGGGTTCAGGTCACGACTCCGCTAGCCATGGCGGTAAGGGAGGATCGAGGGCGGACCAAGAGAGGAAGAAGGGGATACCGTGGACTGAAGAAGAACACAGGTTATTTCTACTTGGTCTAGACAAGTTTGGGAAAGGGGATTGGAGAAGCATTTCAAGAAACTTTGTCATTTCCAGAACTCCAACTCAAGTAGCAAGCCACGCACAAAAATATTTCATCCGTTTGAACTCCATGAACAGAGACAGGAGGAGATCAAGCATCCATGACATTACAAGCGTCAACCACGGAGACGTTTCATCTCACCAGCAGCCGCCAATTACCGGGCAGCAGACCAGTACGTACGCGCCAACTGCGGCCACCACGATAGGAGTAGGAGTAGGACCGCAGTCGGTGAAGCACGGGCCTCAGCCACACATGGCAGGTTTAGGGATGTATGGAGCACCAATGGGGCACCCGGTTTCTGCTCCTCCGGGGCATATGGCGTCCGCCGTGGGCACTCCAGTTATGCTTCCTCCAGGCCACCACCCCCATGCCCATCCTCCATACGTCGTCCCAGTTGCTTACCCAATGGCACATCCAACAATGCACCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Myb_DNA-binding

Protein Analysis

308

Amino Acids

33.08

Weight (kDa)

6.88

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 6 - 54 4.2e-09 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 137 - 182 2.6e-11 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014455)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49010
fragaria_vesca FvH4_5g12670
malus_domestica MD06G1211700.v1.1 MD14G1222200.v1.1
prunus_persica Prupe.5G217700_v2.0.a1
pyrus_communis pycom14g18380
rosa_chinensis RchiOBHm_Chr7g0184661
rosa_laevigata RLG00000004961
rosa_multiflora Rmu_co8449211.1_g000002 Rmu_ssc0000289.1_g000025
rosa_roxburghii Rroxscaffold_3G00269440
rosa_rugosa Rorug06G0464300
rosa_samantha Rh7AG078600 Rh7BG068700 Rh7CG069500 Rh7DG068800
rosa_wichuraiana Rw7G005670

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 740
AccB1I GGYRCC 1 cut(s) 780
AccB7I CCANNNNNTGG 1 cut(s) 66
AccI GTMKAC 1 cut(s) 165
AccII CGCG 1 cut(s) 674
AclWI GGATC 2 cut(s) 77, 382
AcoI YGGCCR 2 cut(s) 259, 684
AcsI RAATTY 1 cut(s) 79
AcuI CTGAAG 1 cut(s) 438
AcyI GRCGYC 1 cut(s) 812
AfaI GTAC 2 cut(s) 667, 671
AfiI CCNNNNNNNGG 2 cut(s) 66, 650
AgsI TTSAA 3 cut(s) 307, 486, 556
AjiI CACGTC 1 cut(s) 172
AjnI CCWGG 1 cut(s) 844
AleI CACNNNNGTG 1 cut(s) 321
AluBI AGCT 2 cut(s) 218, 227
AluI AGCT 2 cut(s) 218, 227
Alw21I GWGCWC 1 cut(s) 772
Alw26I GTCTC 2 cut(s) 564, 612
AlwI GGATC 2 cut(s) 77, 382
AoxI GGCC 4 cut(s) 259, 684, 731, 847
ApeKI GCWGC 3 cut(s) 227, 637, 655
ApoI RAATTY 1 cut(s) 79
ArsI GACNNNNNNTTYG 2 cut(s) 26, 58
Asp700I GAANNNNTTC 1 cut(s) 481
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 3 cut(s) 385, 710, 731
AsuC2I CCSGG 4 cut(s) 280, 652, 785, 801
AsuHPI GGTGA 2 cut(s) 623, 733
AsuNHI GCTAGC 1 cut(s) 353
AvaII GGWCC 2 cut(s) 385, 710
BaeGI GKGCMC 2 cut(s) 783, 827
BalI TGGCCA 1 cut(s) 261
BanI GGYRCC 1 cut(s) 780
BbsI GAAGAC 1 cut(s) 39
Bbv12I GWGCWC 1 cut(s) 772
BbvCI CCTCAGC 1 cut(s) 735
BbvI GCAGC 3 cut(s) 214, 649, 667
BccI CCATC 2 cut(s) 270, 873
BceAI ACGGC 1 cut(s) 803
BcgI CGANNNNNNTGC 2 cut(s) 158, 192
BciT130I CCWGG 1 cut(s) 846
BciVI GTATCC 1 cut(s) 399
BcnI CCSGG 4 cut(s) 280, 652, 785, 801
BcoDI GTCTC 2 cut(s) 564, 612
BfaI CTAG 4 cut(s) 12, 161, 354, 449
BfuAI ACCTGC 1 cut(s) 740
BfuI GTATCC 1 cut(s) 399
BisI GCNGC 5 cut(s) 228, 638, 641, 656, 684
BlsI GCNGC 5 cut(s) 229, 639, 642, 657, 685
Bme1390I CCNGG 5 cut(s) 280, 652, 785, 801, 846
Bme18I GGWCC 2 cut(s) 385, 710
BmgBI CACGTC 1 cut(s) 172
BmgT120I GGNCC 3 cut(s) 385, 710, 731
BmiI GGNNCC 2 cut(s) 117, 782
BmrFI CCNGG 5 cut(s) 280, 652, 785, 801, 846
BmrI ACTGGG 1 cut(s) 878
BmsI GCATC 1 cut(s) 597
BmtI GCTAGC 1 cut(s) 357
BmuI ACTGGG 1 cut(s) 878
BoxI GACNNNNGTC 1 cut(s) 715
BpiI GAAGAC 1 cut(s) 39
BpmI CTGGAG 2 cut(s) 813, 828
Bpu10I CCTNAGC 1 cut(s) 735
BpuEI CTTGAG 1 cut(s) 501
BpuMI CCSGG 4 cut(s) 280, 652, 785, 801
Bsa29I ATCGAT 1 cut(s) 72
BsaAI YACGTR 1 cut(s) 669
BsaHI GRCGYC 1 cut(s) 812
BsaJI CCNNGG 5 cut(s) 358, 410, 613, 800, 819
BsaXI ACNNNNNCTCC 2 cut(s) 690, 720
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 650
Bse1I ACTGG 4 cut(s) 246, 663, 830, 884
BseBI CCWGG 1 cut(s) 846
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 5 cut(s) 358, 410, 613, 800, 819
BseGI GGATG 5 cut(s) 546, 588, 765, 865, 904
BseLI CCNNNNNNNGG 2 cut(s) 66, 650
BseMII CTCAG 2 cut(s) 99, 749
BseNI ACTGG 4 cut(s) 246, 663, 830, 884
BseRI GAGGAG 3 cut(s) 149, 592, 786
BseSI GKGCMC 2 cut(s) 783, 827
BseXI GCAGC 3 cut(s) 214, 649, 667
Bsh1236I CGCG 1 cut(s) 674
BshFI GGCC 4 cut(s) 261, 686, 733, 849
BshNI GGYRCC 1 cut(s) 780
BshVI ATCGAT 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 772
BsiSI CCGG 4 cut(s) 279, 651, 785, 800
BsiWI CGTACG 1 cut(s) 669
BslFI GGGAC 3 cut(s) 184, 295, 866
BslI CCNNNNNNNGG 2 cut(s) 66, 650
BsmAI GTCTC 2 cut(s) 564, 612
BsmBI CGTCTC 1 cut(s) 612
BsmFI GGGAC 3 cut(s) 184, 295, 866
BsnI GGCC 4 cut(s) 261, 686, 733, 849
Bsp1286I GDGCHC 3 cut(s) 772, 783, 827
Bsp143I GATC 3 cut(s) 69, 374, 581
Bsp19I CCATGG 1 cut(s) 358
BspANI GGCC 4 cut(s) 261, 686, 733, 849
BspCNI CTCAG 2 cut(s) 100, 748
BspDI ATCGAT 1 cut(s) 72
BspFNI CGCG 1 cut(s) 674
BspLI GGNNCC 2 cut(s) 117, 782
BspMI ACCTGC 1 cut(s) 740
BspOI GCTAGC 1 cut(s) 357
BspPI GGATC 2 cut(s) 77, 382
BspT107I GGYRCC 1 cut(s) 780
BsrI ACTGG 4 cut(s) 246, 663, 830, 884
BssECI CCNNGG 5 cut(s) 358, 410, 613, 800, 819
BssMI GATC 3 cut(s) 69, 374, 581
BssNI GRCGYC 1 cut(s) 812
BssT1I CCWWGG 1 cut(s) 358
Bst2UI CCWGG 1 cut(s) 846
Bst4CI ACNGT 4 cut(s) 93, 238, 257, 411
BstACI GRCGYC 1 cut(s) 812
BstBAI YACGTR 1 cut(s) 669
BstC8I GCNNGC 2 cut(s) 355, 526
BstDEI CTNAG 2 cut(s) 108, 735
BstDSI CCRYGG 4 cut(s) 358, 410, 613, 819
BstF5I GGATG 5 cut(s) 546, 588, 765, 865, 904
BstFNI CGCG 1 cut(s) 674
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 3 cut(s) 72, 377, 584
BstMAI GTCTC 2 cut(s) 564, 612
BstMBI GATC 3 cut(s) 69, 374, 581
BstMWI GCNNNNNNNGC 5 cut(s) 21, 185, 224, 530, 680
BstNI CCWGG 1 cut(s) 846
BstPAI GACNNNNGTC 1 cut(s) 715
BstSCI CCNGG 5 cut(s) 278, 650, 783, 799, 844
BstSLI GKGCMC 2 cut(s) 783, 827
BstSNI TACGTA 1 cut(s) 669
BstUI CGCG 1 cut(s) 674
BstV1I GCAGC 3 cut(s) 214, 649, 667
BstV2I GAAGAC 1 cut(s) 39
Bsu15I ATCGAT 1 cut(s) 72
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 4 cut(s) 261, 686, 733, 849
BsuTUI ATCGAT 1 cut(s) 72
BtgI CCRYGG 4 cut(s) 358, 410, 613, 819
BtrI CACGTC 1 cut(s) 172
BtsCI GGATG 5 cut(s) 546, 588, 765, 865, 904
BtsIMutI CAGTG 2 cut(s) 98, 262
BveI ACCTGC 1 cut(s) 740
Cac8I GCNNGC 2 cut(s) 355, 526
CfoI GCGC 1 cut(s) 676
Cfr13I GGNCC 3 cut(s) 385, 710, 731
ClaI ATCGAT 1 cut(s) 72
CseI GACGC 2 cut(s) 595, 801
Csp6I GTAC 2 cut(s) 666, 670
CviAII CATG 7 cut(s) 61, 130, 359, 562, 593, 745, 860
CviQI GTAC 2 cut(s) 666, 670
DdeI CTNAG 2 cut(s) 108, 735
DpnI GATC 3 cut(s) 71, 376, 583
DpnII GATC 3 cut(s) 69, 374, 581
EaeI YGGCCR 2 cut(s) 259, 684
EciI GGCGGA 2 cut(s) 398, 805
Eco105I TACGTA 1 cut(s) 669
Eco130I CCWWGG 1 cut(s) 358
Eco47I GGWCC 2 cut(s) 385, 710
Eco57I CTGAAG 1 cut(s) 438
EcoRII CCWGG 1 cut(s) 844
EcoT14I CCWWGG 1 cut(s) 358
EcoT22I ATGCAT 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 358
Esp3I CGTCTC 1 cut(s) 612
FaeI CATG 7 cut(s) 64, 133, 362, 565, 596, 748, 863
FaqI GGGAC 3 cut(s) 184, 295, 866
FatI CATG 7 cut(s) 60, 129, 358, 561, 592, 744, 859
FauI CCCGC 2 cut(s) 181, 210
FauNDI CATATG 1 cut(s) 807
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 5 cut(s) 228, 638, 641, 656, 684
FokI GGATG 5 cut(s) 533, 575, 772, 852, 891
Fsp4HI GCNGC 5 cut(s) 228, 638, 641, 656, 684
FspBI CTAG 4 cut(s) 12, 161, 354, 449
GlaI GCGC 1 cut(s) 675
GluI GCNGC 5 cut(s) 228, 638, 641, 656, 684
GsuI CTGGAG 2 cut(s) 813, 828
HaeIII GGCC 4 cut(s) 261, 686, 733, 849
HapII CCGG 4 cut(s) 279, 651, 785, 800
HgaI GACGC 2 cut(s) 595, 801
HhaI GCGC 1 cut(s) 676
Hin1I GRCGYC 1 cut(s) 812
Hin1II CATG 7 cut(s) 64, 133, 362, 565, 596, 748, 863
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HincII GTYRAC 2 cut(s) 166, 610
HindII GTYRAC 2 cut(s) 166, 610
HinfI GANTC 1 cut(s) 347
HpaII CCGG 4 cut(s) 279, 651, 785, 800
HphI GGTGA 2 cut(s) 623, 733
Hpy166II GTNNAC 3 cut(s) 166, 414, 610
Hpy188I TCNGA 2 cut(s) 85, 295
Hpy188III TCNNGA 5 cut(s) 46, 344, 449, 486, 504
Hpy8I GTNNAC 3 cut(s) 166, 414, 610
Hpy99I CGWCG 3 cut(s) 170, 173, 881
HpyAV CCTTC 1 cut(s) 394
HpyCH4III ACNGT 4 cut(s) 93, 238, 257, 411
HpyCH4IV ACGT 4 cut(s) 171, 621, 668, 876
HpyCH4V TGCA 3 cut(s) 64, 179, 916
HpyF10VI GCNNNNNNNGC 5 cut(s) 21, 185, 224, 530, 680
HpyF3I CTNAG 2 cut(s) 108, 735
HpySE526I ACGT 4 cut(s) 171, 621, 668, 876
Hsp92I GRCGYC 1 cut(s) 812
Hsp92II CATG 7 cut(s) 64, 133, 362, 565, 596, 748, 863
HspAI GCGC 1 cut(s) 674
Kzo9I GATC 3 cut(s) 69, 374, 581
LmnI GCTCC 3 cut(s) 21, 767, 799
Lsp1109I GCAGC 3 cut(s) 214, 649, 667
LweI GCATC 1 cut(s) 597
MaeI CTAG 4 cut(s) 12, 161, 354, 449
MaeII ACGT 4 cut(s) 171, 621, 668, 876
MaeIII GTNAC 2 cut(s) 314, 341
MalI GATC 3 cut(s) 71, 376, 583
MboI GATC 3 cut(s) 69, 374, 581
MboII GAAGA 8 cut(s) 41, 44, 98, 264, 267, 409, 431, 434
MfeI CAATTG 1 cut(s) 54
MhlI GDGCHC 3 cut(s) 772, 783, 827
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 4 cut(s) 54, 79, 307, 645
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 1 cut(s) 341
MmeI TCCRAC 1 cut(s) 536
Mox20I TGGCCA 1 cut(s) 261
Mph1103I ATGCAT 1 cut(s) 66
MroXI GAANNNNTTC 1 cut(s) 481
MscI TGGCCA 1 cut(s) 261
MseI TTAA 1 cut(s) 140
MslI CAYNNNNRTG 2 cut(s) 321, 858
Msp20I TGGCCA 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 218
MspI CCGG 4 cut(s) 279, 651, 785, 800
MspR9I CCNGG 5 cut(s) 280, 652, 785, 801, 846
MunI CAATTG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 846
MvnI CGCG 1 cut(s) 674
MwoI GCNNNNNNNGC 5 cut(s) 21, 185, 224, 530, 680
NciI CCSGG 4 cut(s) 280, 652, 785, 801
NcoI CCATGG 1 cut(s) 358
NdeI CATATG 1 cut(s) 807
NdeII GATC 3 cut(s) 69, 374, 581
NheI GCTAGC 1 cut(s) 353
NlaIII CATG 7 cut(s) 64, 133, 362, 565, 596, 748, 863
NlaIV GGNNCC 2 cut(s) 117, 782
NmuCI GTSAC 2 cut(s) 314, 341
NsiI ATGCAT 1 cut(s) 66
OliI CACNNNNGTG 1 cut(s) 321
PdmI GAANNNNTTC 1 cut(s) 481
Pfl23II CGTACG 1 cut(s) 669
PflMI CCANNNNNTGG 1 cut(s) 66
PfoI TCCNGGA 1 cut(s) 278
PkrI GCNGC 5 cut(s) 229, 639, 642, 657, 685
PleI GAGTC 1 cut(s) 341
PpsI GAGTC 1 cut(s) 341
Ppu21I YACGTR 1 cut(s) 669
PshAI GACNNNNGTC 1 cut(s) 715
Psp6I CCWGG 1 cut(s) 844
PspGI CCWGG 1 cut(s) 844
PspLI CGTACG 1 cut(s) 669
PspN4I GGNNCC 2 cut(s) 117, 782
PspPI GGNCC 3 cut(s) 385, 710, 731
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 2 cut(s) 667, 671
RsaNI GTAC 2 cut(s) 666, 670
RseI CAYNNNNRTG 2 cut(s) 321, 858
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 140
SatI GCNGC 5 cut(s) 228, 638, 641, 656, 684
Sau3AI GATC 3 cut(s) 69, 374, 581
Sau96I GGNCC 3 cut(s) 385, 710, 731
SchI GAGTC 1 cut(s) 341
ScrFI CCNGG 5 cut(s) 280, 652, 785, 801, 846
SduI GDGCHC 3 cut(s) 772, 783, 827
SfaNI GCATC 1 cut(s) 597
SinI GGWCC 2 cut(s) 385, 710
SmiMI CAYNNNNRTG 2 cut(s) 321, 858
SmlI CTYRAG 1 cut(s) 516
SmoI CTYRAG 1 cut(s) 516
SnaBI TACGTA 1 cut(s) 669
Sse9I AATT 4 cut(s) 54, 79, 307, 645
SspI AATATT 1 cut(s) 542
SspMI CTAG 4 cut(s) 12, 161, 354, 449
StyD4I CCNGG 5 cut(s) 278, 650, 783, 799, 844
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 4 cut(s) 93, 238, 257, 411
TaiI ACGT 4 cut(s) 174, 624, 671, 879
TaqI TCGA 6 cut(s) 5, 45, 72, 165, 183, 377
TasI AATT 4 cut(s) 54, 79, 307, 645
TauI GCSGC 2 cut(s) 643, 686
Tru1I TTAA 1 cut(s) 140
Tru9I TTAA 1 cut(s) 140
TscAI CASTG 2 cut(s) 98, 262
TseFI GTSAC 2 cut(s) 314, 341
TseI GCWGC 3 cut(s) 227, 637, 655
Tsp45I GTSAC 2 cut(s) 314, 341
TspDTI ATGAA 5 cut(s) 90, 237, 535, 578, 615
TspGWI ACGGA 3 cut(s) 333, 539, 630
TspRI CASTG 2 cut(s) 98, 262
Van91I CCANNNNNTGG 1 cut(s) 66
VpaK11BI GGWCC 2 cut(s) 385, 710
XapI RAATTY 1 cut(s) 79
XbaI TCTAGA 1 cut(s) 448
XmiI GTMKAC 1 cut(s) 165
XmnI GAANNNNTTC 1 cut(s) 481
XspI CTAG 4 cut(s) 12, 161, 354, 449
Zsp2I ATGCAT 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.