MD14G1222400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0015156
Physical Location & Seq
Reverse (-)
30402944 .. 30404586
1643 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1222400.v1.1.491

Sequence Viewer

Length: 624 bp
ATGGAGGATTCCGGTGCGATCCTCTGCCAAATCTCAACCTACAAGGACATGCTCGACCAGGTGAACGAAGAAATCGAAGCGAATATTCAGATAACGCGGGAGATCGAGTCGGAGATCGTCAAATGCTCCGAAATCGAAAGCGCTCTGTCCGCTAAAGAATCCGACCTCACCAAGACGCTCTACCTTTCGCACTTTGAGCTCACCGGACTACTCACCGTCGCCAAGGATTTGAGGAACTCGGTTGAGGTTTTGGAGAAGGAATTGCATTGTCTAAGAGCAAATCGCGATGAGACTCTTCGCAGAATCCATGACCAGCGGGAAAGGTTTATCGCATTGTGCTTAGAGTTCCAGAAGGACATCGAGAATGGTAACGATAACGAATTACAAGCTCTTATGTCGGAAAAAGAGTTCCTCGAAAATGAAATTCGCTTATTGGAAGAGAAGAACAATGGTCTGAAGAACTCAATGCTGGCATTTGTTGAAGGAATTCTCGAAGATCTTAATAGTTGCAACTCCGCTTTACAAGTTGGGATACGGAGGGGGAACCAGGAGAACGAGAAGCTGCTGAAGGATATCGATAATATGACAACTTCATTGCTTTCGAGTTTCAACTACTGCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

23.74

Weight (kDa)

4.57

Isoelectric Point (pI)

51.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015156)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G61415 AT1G61415 AT1G61450
fragaria_vesca FvH4_5g12640 FvH4_5g12640 FvH4_5g12640
malus_domestica MD14G1222400.v1.1
prunus_persica Prupe.5G218000_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0184691
rosa_laevigata RLG00000004959
rosa_multiflora Rmu_ssc0000289.1_g000023
rosa_samantha Rh7AG078500 Rh7BG068800 Rh7CG069600 Rh7DG069000
rosa_wichuraiana Rw7G005690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 97, 285
AciI CCGC 4 cut(s) 97, 150, 316, 516
AclWI GGATC 1 cut(s) 13
AcsI RAATTY 2 cut(s) 423, 486
AcuI CTGAAG 2 cut(s) 476, 587
AfeI AGCGCT 1 cut(s) 142
AgsI TTSAA 2 cut(s) 482, 610
AjnI CCWGG 2 cut(s) 57, 546
AloI GAACNNNNNNTCC 2 cut(s) 392, 424
AluBI AGCT 3 cut(s) 199, 389, 562
AluI AGCT 3 cut(s) 199, 389, 562
Alw21I GWGCWC 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 284
AlwI GGATC 1 cut(s) 13
Aor51HI AGCGCT 1 cut(s) 142
ApeKI GCWGC 1 cut(s) 562
ApoI RAATTY 2 cut(s) 423, 486
Asp700I GAANNNNTTC 1 cut(s) 486
AspLEI GCGC 1 cut(s) 143
AsuHPI GGTGA 4 cut(s) 73, 160, 193, 205
BanII GRGCYC 1 cut(s) 201
Bbv12I GWGCWC 1 cut(s) 201
BbvI GCAGC 1 cut(s) 549
BciT130I CCWGG 2 cut(s) 59, 548
BciVI GTATCC 1 cut(s) 525
BcoDI GTCTC 1 cut(s) 284
BfoI RGCGCY 1 cut(s) 144
BfuI GTATCC 1 cut(s) 525
BglII AGATCT 1 cut(s) 496
BisI GCNGC 1 cut(s) 563
BlsI GCNGC 1 cut(s) 564
Bme1390I CCNGG 2 cut(s) 59, 548
BmiI GGNNCC 1 cut(s) 545
BmrFI CCNGG 2 cut(s) 59, 548
Bsa29I ATCGAT 1 cut(s) 576
BsaJI CCNNGG 1 cut(s) 222
BsaWI WCCGGW 2 cut(s) 11, 203
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bse3DI GCAATG 1 cut(s) 593
BseBI CCWGG 2 cut(s) 59, 548
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 1 cut(s) 222
BseMI GCAATG 1 cut(s) 593
BseXI GCAGC 1 cut(s) 549
Bsh1236I CGCG 2 cut(s) 97, 285
BshVI ATCGAT 1 cut(s) 576
BsiHKAI GWGCWC 1 cut(s) 201
BsiSI CCGG 2 cut(s) 12, 204
BsmAI GTCTC 1 cut(s) 284
Bsp1286I GDGCHC 1 cut(s) 201
Bsp143I GATC 4 cut(s) 18, 102, 114, 496
Bsp68I TCGCGA 1 cut(s) 285
BspACI CCGC 4 cut(s) 97, 150, 316, 516
BspDI ATCGAT 1 cut(s) 576
BspFNI CGCG 2 cut(s) 97, 285
BspLI GGNNCC 1 cut(s) 545
BspPI GGATC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 593
BssECI CCNNGG 1 cut(s) 222
BssMI GATC 4 cut(s) 18, 102, 114, 496
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 59, 548
Bst4CI ACNGT 1 cut(s) 217
Bst6I CTCTTC 2 cut(s) 300, 432
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 2 cut(s) 272, 340
BstFNI CGCG 2 cut(s) 97, 285
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 4 cut(s) 21, 105, 117, 499
BstMAI GTCTC 1 cut(s) 284
BstMBI GATC 4 cut(s) 18, 102, 114, 496
BstMWI GCNNNNNNNGC 2 cut(s) 149, 196
BstNI CCWGG 2 cut(s) 59, 548
BstNSI RCATGY 1 cut(s) 52
BstSCI CCNGG 2 cut(s) 57, 546
BstUI CGCG 2 cut(s) 97, 285
BstV1I GCAGC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
Bsu15I ATCGAT 1 cut(s) 576
BsuI GTATCC 1 cut(s) 525
BsuTUI ATCGAT 1 cut(s) 576
BtgZI GCGATG 1 cut(s) 300
BtuMI TCGCGA 1 cut(s) 285
Cac8I GCNNGC 1 cut(s) 471
CfoI GCGC 1 cut(s) 143
ClaI ATCGAT 1 cut(s) 576
CseI GACGC 1 cut(s) 184
CsiI ACCWGGT 1 cut(s) 57
CviAII CATG 2 cut(s) 49, 308
CviJI RGCY 3 cut(s) 199, 389, 562
CviKI_1 RGCY 3 cut(s) 199, 389, 562
DdeI CTNAG 2 cut(s) 272, 340
DpnI GATC 4 cut(s) 20, 104, 116, 498
DpnII GATC 4 cut(s) 18, 102, 114, 496
Eam1104I CTCTTC 2 cut(s) 300, 432
EarI CTCTTC 2 cut(s) 300, 432
Ecl136II GAGCTC 1 cut(s) 199
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 201
Eco32I GATATC 1 cut(s) 574
Eco47III AGCGCT 1 cut(s) 142
Eco53kI GAGCTC 1 cut(s) 199
Eco57I CTGAAG 2 cut(s) 476, 587
EcoICRI GAGCTC 1 cut(s) 199
EcoRI GAATTC 1 cut(s) 486
EcoRII CCWGG 2 cut(s) 57, 546
EcoRV GATATC 1 cut(s) 574
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 2 cut(s) 52, 311
FaiI YATR 4 cut(s) 50, 309, 395, 584
FatI CATG 2 cut(s) 48, 307
FauI CCCGC 2 cut(s) 90, 309
Fnu4HI GCNGC 1 cut(s) 563
FriOI GRGCYC 1 cut(s) 201
Fsp4HI GCNGC 1 cut(s) 563
GlaI GCGC 1 cut(s) 142
GluI GCNGC 1 cut(s) 563
HaeII RGCGCY 1 cut(s) 144
HapII CCGG 2 cut(s) 12, 204
HgaI GACGC 1 cut(s) 184
HhaI GCGC 1 cut(s) 143
Hin1II CATG 2 cut(s) 52, 311
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HinfI GANTC 5 cut(s) 8, 107, 158, 292, 303
HpaII CCGG 2 cut(s) 12, 204
HphI GGTGA 4 cut(s) 73, 160, 193, 205
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 6 cut(s) 90, 112, 130, 163, 400, 456
Hpy188III TCNNGA 4 cut(s) 284, 349, 361, 491
Hpy8I GTNNAC 1 cut(s) 64
Hpy99I CGWCG 1 cut(s) 221
HpyAV CCTTC 4 cut(s) 250, 346, 476, 562
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4V TGCA 2 cut(s) 265, 510
HpyF10VI GCNNNNNNNGC 2 cut(s) 149, 196
HpyF3I CTNAG 2 cut(s) 272, 340
Hsp92II CATG 2 cut(s) 52, 311
HspAI GCGC 1 cut(s) 141
Kzo9I GATC 4 cut(s) 18, 102, 114, 496
LmnI GCTCC 1 cut(s) 131
LpnPI CCDG 9 cut(s) 25, 44, 71, 217, 326, 362, 455, 533, 560
Lsp1109I GCAGC 1 cut(s) 549
MabI ACCWGGT 1 cut(s) 57
MaeIII GTNAC 1 cut(s) 368
MalI GATC 4 cut(s) 20, 104, 116, 498
MboI GATC 4 cut(s) 18, 102, 114, 496
MboII GAAGA 6 cut(s) 80, 287, 449, 454, 469, 506
MflI RGATCY 1 cut(s) 496
MhlI GDGCHC 1 cut(s) 201
MluCI AATT 4 cut(s) 260, 380, 423, 486
MlyI GAGTC 2 cut(s) 116, 286
MmeI TCCRAC 3 cut(s) 90, 186, 378
MnlI CCTC 6 cut(s) 32, 176, 225, 238, 422, 531
MroXI GAANNNNTTC 1 cut(s) 486
MseI TTAA 1 cut(s) 501
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 2 cut(s) 12, 204
MspR9I CCNGG 2 cut(s) 59, 548
MvaI CCWGG 2 cut(s) 59, 548
MvnI CGCG 2 cut(s) 97, 285
MwoI GCNNNNNNNGC 2 cut(s) 149, 196
NdeII GATC 4 cut(s) 18, 102, 114, 496
NlaIII CATG 2 cut(s) 52, 311
NlaIV GGNNCC 1 cut(s) 545
NruI TCGCGA 1 cut(s) 285
NspI RCATGY 1 cut(s) 52
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PdmI GAANNNNTTC 1 cut(s) 486
PfeI GAWTC 3 cut(s) 8, 158, 303
PkrI GCNGC 1 cut(s) 564
PleI GAGTC 2 cut(s) 115, 286
PpsI GAGTC 2 cut(s) 115, 286
Psp124BI GAGCTC 1 cut(s) 201
Psp6I CCWGG 2 cut(s) 57, 546
PspGI CCWGG 2 cut(s) 57, 546
PspN4I GGNNCC 1 cut(s) 545
PsuI RGATCY 1 cut(s) 496
RruI TCGCGA 1 cut(s) 285
SacI GAGCTC 1 cut(s) 201
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 1 cut(s) 563
Sau3AI GATC 4 cut(s) 18, 102, 114, 496
SchI GAGTC 2 cut(s) 116, 286
ScrFI CCNGG 2 cut(s) 59, 548
SduI GDGCHC 1 cut(s) 201
SetI ASST 9 cut(s) 41, 63, 168, 186, 201, 249, 326, 391, 564
SexAI ACCWGGT 1 cut(s) 57
Sse9I AATT 4 cut(s) 260, 380, 423, 486
SsiI CCGC 4 cut(s) 97, 150, 316, 516
SspI AATATT 1 cut(s) 85
SstI GAGCTC 1 cut(s) 201
StyD4I CCNGG 2 cut(s) 57, 546
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 217
TaqI TCGA 9 cut(s) 54, 75, 105, 135, 360, 414, 492, 576, 602
TasI AATT 4 cut(s) 260, 380, 423, 486
TfiI GAWTC 3 cut(s) 8, 158, 303
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TseI GCWGC 1 cut(s) 562
TspDTI ATGAA 2 cut(s) 435, 582
TspGWI ACGGA 1 cut(s) 550
XapI RAATTY 2 cut(s) 423, 486
XceI RCATGY 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.