MD14G1235500.v1.1

Ras-related protein RABA6a-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0011926
Physical Location & Seq
Reverse (-)
31525613 .. 31527915
2303 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1235500.v1.1.491

Sequence Viewer

Length: 678 bp
ATGTTGATGGGTGATTCGATCGATGAAGAGTGTGATTACTTGTTTAAGGCAGTTTTGATCGGAGACTCCGGAGTTGGCAAATCAAATTTGCTGTCCAGGTTTTCCAAAGACGAATTCCGGTTGGATTCCAAGCCCACCATCGGCGTCGAATTCGCTTACCGGAATATCAAGGTCGCTGATAAACTTGTCAAGGCTCAAATTTGGGACACTGCCGGCCAAGAAAGATTTAGAGCAATCACGAGCTCATACTACCGTGGGGCGCTCGGCGCCATTCTAGTGTACGACATTTCCGCGAGACAATCCTTCGAAAACGTGAGAAACTGGCTGCGGGAGCTTCGCGAATTTGGCAACTCCGACATGGTCATCGTCCTCGTCGGAAATAAGTCGGACTTGAGCCAGTCTCGACAGGTCGACGAGGAAGAAGGCAAAAGTTTAGCAGAGGCAGAAGGCTTGTGCTTCATGGAAACTTCTGCGTTAGAGAATGTGAACGTGGAGCAAGTGTTTTTGGATATGGTTTATAAGATTCATGAAATTACTAGGCAAAAGATTTTGGAAGCAAAAGTGGAAGAAACGAGTGCTGCGAGTACAAGTCTTGGTGGTGCGACAGAAACCATCCATATTGGTGATGATCTTGAAGTTACGGCGACTAAAAAATCTAGTTATTGTTGCTCAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005886 GO:0012505 GO:0016020 GO:0031410 GO:0031982 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0071944 GO:0097708
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ras

Protein Analysis

226

Amino Acids

25.08

Weight (kDa)

4.8

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 15 - 141 1.6e-14 ADP-ribosylation factor family
Ras PF00071 16 - 176 4.1e-62 Ras family
Roc PF08477 16 - 130 2e-34 Ras of Complex, Roc, domain of DAPkinase
MMR_HSR1 PF01926 17 - 124 4e-06 50S ribosome-binding GTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011926)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G18200 AT1G73640
fragaria_vesca FvH4_5g17230
malus_domestica MD06G1230900.v1.1 MD14G1235500.v1.1
prunus_persica Prupe.5G232200_v2.0.a1
pyrus_communis pycom06g20230 pycom14g19640
rosa_chinensis RchiOBHm_Chr7g0178561
rosa_laevigata RLG00000005437
rosa_multiflora Rmu_sc0012505.1_g000002 Rmu_sc0021447.1_g000001
rosa_roxburghii Rroxscaffold_3G00274810
rosa_rugosa Rorug06G0417900
rosa_samantha Rh7AG016600 Rh7BG016100 Rh7CG016800 Rh7DG016700
rosa_wichuraiana Rw7G001320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 519
AccB1I GGYRCC 1 cut(s) 266
AccI GTMKAC 1 cut(s) 411
AccII CGCG 2 cut(s) 293, 339
AccIII TCCGGA 1 cut(s) 68
AciI CCGC 2 cut(s) 291, 328
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 5 cut(s) 85, 113, 149, 198, 341
AcyI GRCGYC 2 cut(s) 144, 267
AfaI GTAC 2 cut(s) 281, 586
AfiI CCNNNNNNNGG 1 cut(s) 140
AgsI TTSAA 1 cut(s) 635
AjnI CCWGG 1 cut(s) 95
AjuI GAANNNNNNNTTGG 2 cut(s) 98, 130
AluBI AGCT 2 cut(s) 243, 334
AluI AGCT 2 cut(s) 243, 334
Alw21I GWGCWC 1 cut(s) 245
Alw26I GTCTC 3 cut(s) 57, 289, 405
Aor13HI TCCGGA 1 cut(s) 68
AoxI GGCC 1 cut(s) 214
ApeKI GCWGC 2 cut(s) 325, 578
ApoI RAATTY 5 cut(s) 85, 113, 149, 198, 341
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspLEI GCGC 2 cut(s) 262, 269
AsuHPI GGTGA 2 cut(s) 23, 635
AsuII TTCGAA 1 cut(s) 306
BanI GGYRCC 1 cut(s) 266
BanII GRGCYC 1 cut(s) 245
BauI CACGAG 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 312, 565
BccI CCATC 2 cut(s) 146, 620
BceAI ACGGC 1 cut(s) 657
BciT130I CCWGG 1 cut(s) 97
BcoDI GTCTC 3 cut(s) 57, 289, 405
BfaI CTAG 3 cut(s) 275, 537, 657
BfoI RGCGCY 2 cut(s) 263, 270
BisI GCNGC 2 cut(s) 326, 579
BlsI GCNGC 2 cut(s) 327, 580
Bme1390I CCNGG 1 cut(s) 97
BmiI GGNNCC 1 cut(s) 268
BmrFI CCNGG 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 385, 417
Bpu14I TTCGAA 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 412
Bsa29I ATCGAT 1 cut(s) 21
BsaHI GRCGYC 2 cut(s) 144, 267
BsaJI CCNNGG 1 cut(s) 253
BsaWI WCCGGW 3 cut(s) 68, 117, 159
BsaXI ACNNNNNCTCC 2 cut(s) 485, 515
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse118I RCCGGY 1 cut(s) 212
Bse1I ACTGG 2 cut(s) 326, 397
BseAI TCCGGA 1 cut(s) 68
BseBI CCWGG 1 cut(s) 97
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 1 cut(s) 253
BseGI GGATG 1 cut(s) 612
BseLI CCNNNNNNNGG 1 cut(s) 140
BseNI ACTGG 2 cut(s) 326, 397
BseXI GCAGC 2 cut(s) 312, 565
Bsh1236I CGCG 2 cut(s) 293, 339
Bsh1285I CGRYCG 1 cut(s) 21
BshFI GGCC 1 cut(s) 216
BshNI GGYRCC 1 cut(s) 266
BshVI ATCGAT 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 21
BsiHKAI GWGCWC 1 cut(s) 245
BsiSI CCGG 4 cut(s) 69, 118, 160, 213
BslFI GGGAC 1 cut(s) 218
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 3 cut(s) 57, 289, 405
BsmFI GGGAC 1 cut(s) 218
BsnI GGCC 1 cut(s) 216
Bsp119I TTCGAA 1 cut(s) 306
Bsp1286I GDGCHC 1 cut(s) 245
Bsp13I TCCGGA 1 cut(s) 68
Bsp143I GATC 3 cut(s) 18, 57, 628
Bsp68I TCGCGA 1 cut(s) 339
BspACI CCGC 2 cut(s) 291, 328
BspANI GGCC 1 cut(s) 216
BspDI ATCGAT 1 cut(s) 21
BspEI TCCGGA 1 cut(s) 68
BspFNI CGCG 2 cut(s) 293, 339
BspHI TCATGA 1 cut(s) 526
BspLI GGNNCC 1 cut(s) 268
BspT104I TTCGAA 1 cut(s) 306
BspT107I GGYRCC 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 212
BsrI ACTGG 2 cut(s) 326, 397
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 253
BssMI GATC 3 cut(s) 18, 57, 628
BssNI GRCGYC 2 cut(s) 144, 267
BssSI CACGAG 1 cut(s) 238
Bst2BI CACGAG 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 254
Bst6I CTCTTC 1 cut(s) 21
BstACI GRCGYC 2 cut(s) 144, 267
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 1 cut(s) 214
BstDSI CCRYGG 1 cut(s) 253
BstF5I GGATG 1 cut(s) 612
BstFNI CGCG 2 cut(s) 293, 339
BstH2I RGCGCY 2 cut(s) 263, 270
BstHHI GCGC 2 cut(s) 262, 269
BstKTI GATC 3 cut(s) 21, 60, 631
BstMAI GTCTC 3 cut(s) 57, 289, 405
BstMBI GATC 3 cut(s) 18, 57, 628
BstMCI CGRYCG 1 cut(s) 21
BstMWI GCNNNNNNNGC 3 cut(s) 266, 331, 345
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 95
BstUI CGCG 2 cut(s) 293, 339
BstV1I GCAGC 2 cut(s) 312, 565
Bsu15I ATCGAT 1 cut(s) 21
BsuRI GGCC 1 cut(s) 216
BsuTUI ATCGAT 1 cut(s) 21
BtgI CCRYGG 1 cut(s) 253
BtsCI GGATG 1 cut(s) 612
BtsI GCAGTG 1 cut(s) 207
BtsIMutI CAGTG 1 cut(s) 207
BtuMI TCGCGA 1 cut(s) 339
Cac8I GCNNGC 1 cut(s) 214
CciI TCATGA 1 cut(s) 526
CfoI GCGC 2 cut(s) 262, 269
Cfr10I RCCGGY 1 cut(s) 212
ClaI ATCGAT 1 cut(s) 21
CseI GACGC 1 cut(s) 133
Csp6I GTAC 2 cut(s) 280, 585
CviAII CATG 3 cut(s) 358, 460, 527
CviJI RGCY 8 cut(s) 133, 194, 216, 243, 325, 334, 396, 450
CviKI_1 RGCY 8 cut(s) 133, 194, 216, 243, 325, 334, 396, 450
CviQI GTAC 2 cut(s) 280, 585
DinI GGCGCC 1 cut(s) 268
DpnI GATC 3 cut(s) 20, 59, 630
DpnII GATC 3 cut(s) 18, 57, 628
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 1 cut(s) 21
EarI CTCTTC 1 cut(s) 21
Ecl136II GAGCTC 1 cut(s) 243
Eco24I GRGCYC 1 cut(s) 245
Eco53kI GAGCTC 1 cut(s) 243
EcoICRI GAGCTC 1 cut(s) 243
EcoRI GAATTC 2 cut(s) 113, 149
EcoRII CCWGG 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 245
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
FaeI CATG 3 cut(s) 361, 463, 530
FaiI YATR 7 cut(s) 247, 359, 461, 512, 519, 528, 618
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 1 cut(s) 218
FatI CATG 3 cut(s) 357, 459, 526
FauI CCCGC 1 cut(s) 321
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 2 cut(s) 326, 579
FokI GGATG 1 cut(s) 599
FriOI GRGCYC 1 cut(s) 245
Fsp4HI GCNGC 2 cut(s) 326, 579
FspBI CTAG 3 cut(s) 275, 537, 657
GlaI GCGC 2 cut(s) 261, 268
GluI GCNGC 2 cut(s) 326, 579
HaeII RGCGCY 2 cut(s) 263, 270
HaeIII GGCC 1 cut(s) 216
HapII CCGG 4 cut(s) 69, 118, 160, 213
HgaI GACGC 1 cut(s) 133
HhaI GCGC 2 cut(s) 262, 269
Hin1I GRCGYC 2 cut(s) 144, 267
Hin1II CATG 3 cut(s) 361, 463, 530
Hin6I GCGC 2 cut(s) 260, 267
HinP1I GCGC 2 cut(s) 260, 267
HincII GTYRAC 1 cut(s) 412
HindII GTYRAC 1 cut(s) 412
HinfI GANTC 4 cut(s) 14, 65, 125, 523
HpaII CCGG 4 cut(s) 69, 118, 160, 213
HphI GGTGA 2 cut(s) 23, 635
Hpy166II GTNNAC 3 cut(s) 280, 412, 487
Hpy188I TCNGA 4 cut(s) 62, 355, 377, 388
Hpy188III TCNNGA 6 cut(s) 69, 238, 338, 402, 527, 632
Hpy8I GTNNAC 3 cut(s) 280, 412, 487
Hpy99I CGWCG 3 cut(s) 149, 377, 416
HpyAV CCTTC 3 cut(s) 313, 416, 440
HpyCH4III ACNGT 1 cut(s) 254
HpyCH4IV ACGT 2 cut(s) 312, 489
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 331, 345
HpySE526I ACGT 2 cut(s) 312, 489
Hsp92I GRCGYC 2 cut(s) 144, 267
Hsp92II CATG 3 cut(s) 361, 463, 530
HspAI GCGC 2 cut(s) 260, 267
KasI GGCGCC 1 cut(s) 266
Kpn2I TCCGGA 1 cut(s) 68
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Kzo9I GATC 3 cut(s) 18, 57, 628
LmnI GCTCC 2 cut(s) 331, 493
LpnPI CCDG 9 cut(s) 82, 82, 109, 131, 173, 226, 307, 392, 410
Lsp1109I GCAGC 2 cut(s) 312, 565
MaeI CTAG 3 cut(s) 275, 537, 657
MaeII ACGT 2 cut(s) 312, 489
MaeIII GTNAC 1 cut(s) 637
MalI GATC 3 cut(s) 20, 59, 630
MboI GATC 3 cut(s) 18, 57, 628
MboII GAAGA 3 cut(s) 38, 431, 578
MhlI GDGCHC 1 cut(s) 245
MluCI AATT 7 cut(s) 85, 113, 149, 198, 341, 531, 673
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 4 cut(s) 102, 355, 366, 378
MnlI CCTC 3 cut(s) 380, 409, 433
MroI TCCGGA 1 cut(s) 68
MroNI GCCGGC 1 cut(s) 212
MseI TTAA 1 cut(s) 45
MslI CAYNNNNRTG 2 cut(s) 275, 621
MspI CCGG 4 cut(s) 69, 118, 160, 213
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MvnI CGCG 2 cut(s) 293, 339
MwoI GCNNNNNNNGC 3 cut(s) 266, 331, 345
NaeI GCCGGC 1 cut(s) 214
NarI GGCGCC 1 cut(s) 267
NdeII GATC 3 cut(s) 18, 57, 628
NgoMIV GCCGGC 1 cut(s) 212
NlaIII CATG 3 cut(s) 361, 463, 530
NlaIV GGNNCC 1 cut(s) 268
NmeAIII GCCGAG 1 cut(s) 243
NruI TCGCGA 1 cut(s) 339
NspV TTCGAA 1 cut(s) 306
PagI TCATGA 1 cut(s) 526
PcsI WCGNNNNNNNCGW 1 cut(s) 578
PdiI GCCGGC 1 cut(s) 214
PfeI GAWTC 3 cut(s) 14, 125, 523
PflFI GACNNNGTC 1 cut(s) 359
PkrI GCNGC 2 cut(s) 327, 580
Ple19I CGATCG 1 cut(s) 21
PleI GAGTC 1 cut(s) 59
PluTI GGCGCC 1 cut(s) 270
PpsI GAGTC 1 cut(s) 59
PsiI TTATAA 1 cut(s) 519
Psp124BI GAGCTC 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 268
PsyI GACNNNGTC 1 cut(s) 359
PvuI CGATCG 1 cut(s) 21
RruI TCGCGA 1 cut(s) 339
RsaI GTAC 2 cut(s) 281, 586
RsaNI GTAC 2 cut(s) 280, 585
RseI CAYNNNNRTG 2 cut(s) 275, 621
SacI GAGCTC 1 cut(s) 245
SalI GTCGAC 1 cut(s) 410
SaqAI TTAA 1 cut(s) 45
SatI GCNGC 2 cut(s) 326, 579
Sau3AI GATC 3 cut(s) 18, 57, 628
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 1 cut(s) 245
SetI ASST 7 cut(s) 101, 174, 245, 315, 336, 411, 492
SfoI GGCGCC 1 cut(s) 268
SfuI TTCGAA 1 cut(s) 306
SmiMI CAYNNNNRTG 2 cut(s) 275, 621
SmlI CTYRAG 1 cut(s) 391
SmoI CTYRAG 1 cut(s) 391
Sse9I AATT 7 cut(s) 85, 113, 149, 198, 341, 531, 673
SsiI CCGC 2 cut(s) 291, 328
SspDI GGCGCC 1 cut(s) 266
SspMI CTAG 3 cut(s) 275, 537, 657
SstI GAGCTC 1 cut(s) 245
StyD4I CCNGG 1 cut(s) 95
TaaI ACNGT 1 cut(s) 254
TaiI ACGT 2 cut(s) 315, 492
TaqI TCGA 6 cut(s) 17, 21, 147, 306, 403, 411
TasI AATT 7 cut(s) 85, 113, 149, 198, 341, 531, 673
TatI WGTACW 1 cut(s) 584
TfiI GAWTC 3 cut(s) 14, 125, 523
Tru1I TTAA 1 cut(s) 45
Tru9I TTAA 1 cut(s) 45
TscAI CASTG 1 cut(s) 214
TseI GCWGC 2 cut(s) 325, 578
TspDTI ATGAA 4 cut(s) 39, 448, 515, 543
TspRI CASTG 1 cut(s) 214
Tth111I GACNNNGTC 1 cut(s) 359
XapI RAATTY 5 cut(s) 85, 113, 149, 198, 341
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 3 cut(s) 275, 537, 657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.