Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0009510

Physical Location & Seq

Genomic Coordinates Reverse (-)

31830433 .. 31834642

Gene Structure 4210 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1239300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1275 bp

ATGGCGTCTATCAGCTTTACTCAGATCGGAGGCAAAAACCCAGTTGCTGAATTTTCCAGGAGAACTCGTCCTGAGGTGCGTTTAACTCGGCCAAGCCGAGTAGGGTTCAGGGTCTTTGCCTCCGAGAGCAAGGCGGAGCCTGATCTTAGTGTTACTGTTAACGGGTTGCACATGCCCAACCCGTTTGTTATCGGGTCGGGTCCGCCGGGGACTAACTATACGGTGATGAAGAGGGCCTTTGATGAAGGCTGGGGTGCTGTGATTGCCAAAACTGTATCACTAGAGGCAGACAAAGTAAAAAATGTCACTCCTCGATATGCTCGGCTACGAGTAGATGGAAATGGCTCAGCCAAAGGGCAAATTATTGGGTGGGAGAACATAGAACTGATAAGTGATCGACCTCTTGATATTATGTTGAAAGAATTCAAGCAATTAAAAGAAGAGTATCCAGATAGGATTCTGATTGCTTCAATTATGGAGGAATACAACAAAGCTGCCTGGGAGGAACTTATTGACCGTGTTGAGCAAACTGGAGTTGATGCTCTTGAAATCAATTTTTCCTGTCCTCATGGTATGCCAGAGCGCAAGATGGGTGCTGCAGTTGGTCAGGACTGTGTACTTCTGGAAGAAGTTTGTGGCTGGATCAATGCCAAAGCCACAGTTCCTGTTTGGGCAAAGATGACTCCTAACATTACTGACATCACACAGCCAGCTAGAGTCGCTCTGAGCTCAGGATGCGAGGGCGTGTCAGCTATTAATACCATCATGAGTGTAATGGGAATCAATCTAACTACCTTACGGCCCGAGCCTTGTGTGGAGGGATACTCAACTCCTGGGGGCTATTCGGCTAAAGCGGTTCATCCAATTGCACTGGCGAAAGTGATGAACATTGCAAAGTTAATGAAATCAGAGTTTGGTGATACGGATTACTCGCTTTCTGGTATTGGGGGTGTTGAGTCTGGTGGCGATGCTGCTGAGTTTATTCTTCTTGGAGCAAATACTGTTCAGGTATGCACTGGGGTCATGATGCATGGATATGGTGTTGTGAAGAAACTTTGTTCCGAGCTGAAGGATTTTATGAAACAGCACAATTTTTCATCCATTGAAGATTTCAGGGGGGCTTCCCTCGAATACTTTACAACACATACAGATTTAGTAAGAAGACAACAGGAGGCAATCAAACAGAGGAAAGCCATCCGAAAAGGATTGCAATCCGATAAGGACTGGACAGGAGACGGCTTCGTGAAAGAGACCGAGAGCATGGTTTCCAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003954 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006206 GO:0006208 GO:0006212 GO:0006725 GO:0006807 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016491 GO:0016627 GO:0016628 GO:0016651 GO:0017113 GO:0017144 GO:0019860 GO:0031667 GO:0031668 GO:0031669 GO:0034641 GO:0042737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046113 GO:0046483 GO:0046700 GO:0048037 GO:0050896 GO:0051536 GO:0051540 GO:0051716 GO:0055086 GO:0055114 GO:0071496 GO:0071704 GO:0072527 GO:0072529 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100

Pfam Domains

Protein Families

DHO_dh

Protein Analysis

425

Amino Acids

46.44

Weight (kDa)

6.16

Isoelectric Point (pI)

39.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHO_dh	PF01180	49 - 360	8.5e-35	Dihydroorotate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009510)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17810
fragaria_vesca	FvH4_5g17680 FvH4_5g17680
malus_domestica	MD06G1232500.v1.1 MD14G1239300.v1.1
prunus_persica	Prupe.5G236800_v2.0.a1 Prupe.5G236800_v2.0.a1 Prupe.5G236800_v2.0.a1
pyrus_communis	pycom06g20670
rosa_chinensis	RchiOBHm_Chr7g0177971
rosa_laevigata	RLG00000005486
rosa_multiflora	Rmu_co8250821.1_g000001 Rmu_sc0022313.1_g000001 Rmu_sc0034489.1_g000001
rosa_roxburghii	Rroxscaffold_3G00275350
rosa_rugosa	Rorug06G0411900 Rorug06G0411900
rosa_samantha	Rh7AG010900 Rh7BG010800 Rh7CG011200 Rh7DG010900
rosa_wichuraiana	Rw7G000910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	134, 203, 854
AclWI	GGATC	1 cut(s)	650
AcoI	YGGCCR	1 cut(s)	89
AcsI	RAATTY	2 cut(s)	50, 422
AcuI	CTGAAG	1 cut(s)	1088
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	1 cut(s)	618
AgsI	TTSAA	5 cut(s)	418, 427, 471, 548, 1106
AjnI	CCWGG	3 cut(s)	56, 497, 832
AluBI	AGCT	6 cut(s)	15, 494, 713, 729, 752, 1066
AluI	AGCT	6 cut(s)	15, 494, 713, 729, 752, 1066
Alw21I	GWGCWC	1 cut(s)	731
Alw26I	GTCTC	2 cut(s)	1227, 1244
AlwI	GGATC	1 cut(s)	650
AlwNI	CAGNNNCTG	2 cut(s)	47, 665
Ama87I	CYCGRG	1 cut(s)	803
AoxI	GGCC	3 cut(s)	89, 234, 800
ApeKI	GCWGC	3 cut(s)	494, 596, 971
ApoI	RAATTY	2 cut(s)	50, 422
AseI	ATTAAT	1 cut(s)	756
Asp700I	GAANNNNTTC	1 cut(s)	422
AspLEI	GCGC	1 cut(s)	585
AspS9I	GGNCC	3 cut(s)	200, 234, 801
AsuC2I	CCSGG	1 cut(s)	207
AsuHPI	GGTGA	2 cut(s)	235, 929
AvaI	CYCGRG	1 cut(s)	803
AvaII	GGWCC	1 cut(s)	200
AxyI	CCTNAGG	1 cut(s)	72
BanII	GRGCYC	1 cut(s)	731
BbsI	GAAGAC	1 cut(s)	1168
Bbv12I	GWGCWC	1 cut(s)	731
BbvI	GCAGC	3 cut(s)	481, 583, 958
BccI	CCATC	4 cut(s)	329, 583, 770, 1202
BceAI	ACGGC	2 cut(s)	815, 1252
BciT130I	CCWGG	3 cut(s)	58, 499, 834
BciVI	GTATCC	2 cut(s)	456, 815
BcnI	CCSGG	1 cut(s)	207
BcoDI	GTCTC	2 cut(s)	1227, 1244
BfaI	CTAG	2 cut(s)	281, 714
BfmI	CTRYAG	1 cut(s)	597
BfuI	GTATCC	2 cut(s)	456, 815
BisI	GCNGC	3 cut(s)	495, 597, 972
BlpI	GCTNAGC	1 cut(s)	346
BlsI	GCNGC	3 cut(s)	496, 598, 973
Bme1390I	CCNGG	4 cut(s)	58, 207, 499, 834
Bme18I	GGWCC	1 cut(s)	200
BmeT110I	CYCGRG	1 cut(s)	803
BmgT120I	GGNCC	3 cut(s)	200, 234, 801
BmiI	GGNNCC	2 cut(s)	138, 201
BmrFI	CCNGG	4 cut(s)	58, 207, 499, 834
BmrI	ACTGGG	2 cut(s)	35, 1026
BmsI	GCATC	4 cut(s)	529, 725, 958, 1017
BmuI	ACTGGG	2 cut(s)	35, 1026
BpiI	GAAGAC	1 cut(s)	1168
BplI	GAGNNNNNCTC	2 cut(s)	809, 841
BpmI	CTGGAG	1 cut(s)	552
Bpu10I	CCTNAGC	1 cut(s)	730
Bpu1102I	GCTNAGC	1 cut(s)	346
BpuMI	CCSGG	1 cut(s)	207
BsaBI	GATNNNNATC	1 cut(s)	1210
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	1 cut(s)	1244
BsaJI	CCNNGG	3 cut(s)	206, 498, 833
BsaXI	ACNNNNNCTCC	2 cut(s)	52, 82
Bse1I	ACTGG	5 cut(s)	41, 535, 876, 1021, 1229
Bse21I	CCTNAGG	1 cut(s)	72
Bse3DI	GCAATG	1 cut(s)	888
Bse8I	GATNNNNATC	1 cut(s)	1210
BseBI	CCWGG	3 cut(s)	58, 499, 834
BseDI	CCNNGG	3 cut(s)	206, 498, 833
BseGI	GGATG	4 cut(s)	740, 859, 1097, 1194
BseJI	GATNNNNATC	1 cut(s)	1210
BseMI	GCAATG	1 cut(s)	888
BseMII	CTCAG	6 cut(s)	35, 63, 360, 716, 744, 966
BseNI	ACTGG	5 cut(s)	41, 535, 876, 1021, 1229
BseRI	GAGGAG	1 cut(s)	300
BseXI	GCAGC	3 cut(s)	481, 583, 958
BseYI	CCCAGC	1 cut(s)	249
BshFI	GGCC	3 cut(s)	91, 236, 802
BsiHKAI	GWGCWC	1 cut(s)	731
BsiHKCI	CYCGRG	1 cut(s)	803
BsiSI	CCGG	1 cut(s)	206
BslFI	GGGAC	1 cut(s)	223
BsmAI	GTCTC	2 cut(s)	1227, 1244
BsmBI	CGTCTC	1 cut(s)	1227
BsmFI	GGGAC	1 cut(s)	223
BsnI	GGCC	3 cut(s)	91, 236, 802
Bso31I	GGTCTC	1 cut(s)	1244
BsoBI	CYCGRG	1 cut(s)	803
Bsp1286I	GDGCHC	1 cut(s)	731
Bsp143I	GATC	4 cut(s)	24, 142, 394, 642
Bsp1720I	GCTNAGC	1 cut(s)	346
BspACI	CCGC	3 cut(s)	134, 203, 854
BspANI	GGCC	3 cut(s)	91, 236, 802
BspCNI	CTCAG	6 cut(s)	34, 64, 359, 717, 743, 967
BspHI	TCATGA	2 cut(s)	765, 1023
BspLI	GGNNCC	2 cut(s)	138, 201
BspMAI	CTGCAG	1 cut(s)	601
BspPI	GGATC	1 cut(s)	650
BspTNI	GGTCTC	1 cut(s)	1244
BsrDI	GCAATG	1 cut(s)	888
BsrI	ACTGG	5 cut(s)	41, 535, 876, 1021, 1229
BssECI	CCNNGG	3 cut(s)	206, 498, 833
BssMI	GATC	4 cut(s)	24, 142, 394, 642
BssNI	GRCGYC	1 cut(s)	5
Bst2UI	CCWGG	3 cut(s)	58, 499, 834
Bst4CI	ACNGT	7 cut(s)	157, 223, 274, 518, 614, 661, 1003
Bst6I	CTCTTC	2 cut(s)	224, 435
BstACI	GRCGYC	1 cut(s)	5
BstC8I	GCNNGC	1 cut(s)	711
BstDEI	CTNAG	7 cut(s)	21, 72, 146, 346, 725, 730, 975
BstF5I	GGATG	4 cut(s)	740, 859, 1097, 1194
BstHHI	GCGC	1 cut(s)	585
BstKTI	GATC	4 cut(s)	27, 145, 397, 645
BstMAI	GTCTC	2 cut(s)	1227, 1244
BstMBI	GATC	4 cut(s)	24, 142, 394, 642
BstMWI	GCNNNNNNNGC	3 cut(s)	263, 719, 735
BstNI	CCWGG	3 cut(s)	58, 499, 834
BstNSI	RCATGY	1 cut(s)	175
BstSCI	CCNGG	4 cut(s)	56, 205, 497, 832
BstSFI	CTRYAG	1 cut(s)	597
BstV1I	GCAGC	3 cut(s)	481, 583, 958
BstV2I	GAAGAC	1 cut(s)	1168
Bsu36I	CCTNAGG	1 cut(s)	72
BsuI	GTATCC	2 cut(s)	456, 815
BsuRI	GGCC	3 cut(s)	91, 236, 802
BtgZI	GCGATG	1 cut(s)	981
BtsCI	GGATG	4 cut(s)	740, 859, 1097, 1194
BtsIMutI	CAGTG	2 cut(s)	869, 1014
Cac8I	GCNNGC	1 cut(s)	711
CaiI	CAGNNNCTG	2 cut(s)	47, 665
CciI	TCATGA	2 cut(s)	765, 1023
CfoI	GCGC	1 cut(s)	585
Cfr13I	GGNCC	3 cut(s)	200, 234, 801
Csp6I	GTAC	1 cut(s)	617
CviAII	CATG	6 cut(s)	172, 569, 766, 1024, 1031, 1261
CviQI	GTAC	1 cut(s)	617
DdeI	CTNAG	7 cut(s)	21, 72, 146, 346, 725, 730, 975
DpnI	GATC	4 cut(s)	26, 144, 396, 644
DpnII	GATC	4 cut(s)	24, 142, 394, 642
EaeI	YGGCCR	1 cut(s)	89
Eam1104I	CTCTTC	2 cut(s)	224, 435
EarI	CTCTTC	2 cut(s)	224, 435
EciI	GGCGGA	2 cut(s)	149, 192
Ecl136II	GAGCTC	1 cut(s)	729
Eco24I	GRGCYC	1 cut(s)	731
Eco31I	GGTCTC	1 cut(s)	1244
Eco47I	GGWCC	1 cut(s)	200
Eco53kI	GAGCTC	1 cut(s)	729
Eco57I	CTGAAG	1 cut(s)	1088
Eco81I	CCTNAGG	1 cut(s)	72
Eco88I	CYCGRG	1 cut(s)	803
EcoICRI	GAGCTC	1 cut(s)	729
EcoO109I	RGGNCCY	1 cut(s)	234
EcoRI	GAATTC	1 cut(s)	422
EcoRII	CCWGG	3 cut(s)	56, 497, 832
EcoT22I	ATGCAT	1 cut(s)	1032
EcoT38I	GRGCYC	1 cut(s)	731
Esp3I	CGTCTC	1 cut(s)	1227
FaeI	CATG	6 cut(s)	175, 572, 769, 1027, 1034, 1264
FalI	AAGNNNNNCTT	2 cut(s)	221, 253
FaqI	GGGAC	1 cut(s)	223
FatI	CATG	6 cut(s)	171, 568, 765, 1023, 1030, 1260
Fnu4HI	GCNGC	3 cut(s)	495, 597, 972
FokI	GGATG	4 cut(s)	747, 846, 1084, 1181
FriOI	GRGCYC	1 cut(s)	731
Fsp4HI	GCNGC	3 cut(s)	495, 597, 972
FspBI	CTAG	2 cut(s)	281, 714
GlaI	GCGC	1 cut(s)	584
GluI	GCNGC	3 cut(s)	495, 597, 972
GsaI	CCCAGC	1 cut(s)	253
GsuI	CTGGAG	1 cut(s)	552
HaeIII	GGCC	3 cut(s)	91, 236, 802
HapII	CCGG	1 cut(s)	206
HhaI	GCGC	1 cut(s)	585
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	6 cut(s)	175, 572, 769, 1027, 1034, 1264
Hin6I	GCGC	1 cut(s)	583
HinP1I	GCGC	1 cut(s)	583
HincII	GTYRAC	1 cut(s)	160
HindII	GTYRAC	1 cut(s)	160
HinfI	GANTC	5 cut(s)	457, 682, 717, 780, 956
HpaI	GTTAAC	1 cut(s)	160
HpaII	CCGG	1 cut(s)	206
HphI	GGTGA	2 cut(s)	235, 929
Hpy166II	GTNNAC	2 cut(s)	160, 617
Hpy188I	TCNGA	9 cut(s)	24, 29, 124, 462, 726, 910, 1063, 1199, 1216
Hpy8I	GTNNAC	2 cut(s)	160, 617
HpyAV	CCTTC	2 cut(s)	239, 1063
HpyCH4III	ACNGT	7 cut(s)	157, 223, 274, 518, 614, 661, 1003
HpyCH4V	TGCA	7 cut(s)	169, 599, 869, 893, 1014, 1030, 1210
HpyF10VI	GCNNNNNNNGC	3 cut(s)	263, 719, 735
HpyF3I	CTNAG	7 cut(s)	21, 72, 146, 346, 725, 730, 975
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	6 cut(s)	175, 572, 769, 1027, 1034, 1264
HspAI	GCGC	1 cut(s)	583
KspAI	GTTAAC	1 cut(s)	160
Kzo9I	GATC	4 cut(s)	24, 142, 394, 642
LmnI	GCTCC	2 cut(s)	136, 992
Lsp1109I	GCAGC	3 cut(s)	481, 583, 958
LweI	GCATC	4 cut(s)	529, 725, 958, 1017
MaeI	CTAG	2 cut(s)	281, 714
MaeIII	GTNAC	2 cut(s)	151, 304
MalI	GATC	4 cut(s)	26, 144, 396, 644
MboI	GATC	4 cut(s)	24, 142, 394, 642
MboII	GAAGA	7 cut(s)	241, 452, 638, 977, 1060, 1118, 1173
MfeI	CAATTG	1 cut(s)	864
MhlI	GDGCHC	1 cut(s)	731
MluCI	AATT	8 cut(s)	50, 360, 422, 431, 471, 553, 864, 1090
MlyI	GAGTC	3 cut(s)	676, 726, 965
Mph1103I	ATGCAT	1 cut(s)	1032
MroXI	GAANNNNTTC	1 cut(s)	422
MseI	TTAA	5 cut(s)	83, 159, 434, 756, 899
MslI	CAYNNNNRTG	1 cut(s)	1035
MspI	CCGG	1 cut(s)	206
MspR9I	CCNGG	4 cut(s)	58, 207, 499, 834
MunI	CAATTG	1 cut(s)	864
MvaI	CCWGG	3 cut(s)	58, 499, 834
MwoI	GCNNNNNNNGC	3 cut(s)	263, 719, 735
NciI	CCSGG	1 cut(s)	207
NdeII	GATC	4 cut(s)	24, 142, 394, 642
NlaIII	CATG	6 cut(s)	175, 572, 769, 1027, 1034, 1264
NlaIV	GGNNCC	2 cut(s)	138, 201
NmeAIII	GCCGAG	3 cut(s)	67, 122, 301
NmuCI	GTSAC	1 cut(s)	304
NsiI	ATGCAT	1 cut(s)	1032
NspI	RCATGY	1 cut(s)	175
PagI	TCATGA	2 cut(s)	765, 1023
PcsI	WCGNNNNNNNCGW	1 cut(s)	94
PdmI	GAANNNNTTC	1 cut(s)	422
PfeI	GAWTC	2 cut(s)	457, 780
PfoI	TCCNGGA	1 cut(s)	56
PkrI	GCNGC	3 cut(s)	496, 598, 973
PleI	GAGTC	3 cut(s)	676, 725, 964
PpsI	GAGTC	3 cut(s)	676, 725, 964
PshBI	ATTAAT	1 cut(s)	756
Psp124BI	GAGCTC	1 cut(s)	731
Psp6I	CCWGG	3 cut(s)	56, 497, 832
PspFI	CCCAGC	1 cut(s)	249
PspGI	CCWGG	3 cut(s)	56, 497, 832
PspN4I	GGNNCC	2 cut(s)	138, 201
PspPI	GGNCC	3 cut(s)	200, 234, 801
PstI	CTGCAG	1 cut(s)	601
PstNI	CAGNNNCTG	2 cut(s)	47, 665
RsaI	GTAC	1 cut(s)	618
RsaNI	GTAC	1 cut(s)	617
RseI	CAYNNNNRTG	1 cut(s)	1035
SacI	GAGCTC	1 cut(s)	731
SaqAI	TTAA	5 cut(s)	83, 159, 434, 756, 899
SatI	GCNGC	3 cut(s)	495, 597, 972
Sau3AI	GATC	4 cut(s)	24, 142, 394, 642
Sau96I	GGNCC	3 cut(s)	200, 234, 801
SchI	GAGTC	3 cut(s)	676, 726, 965
ScrFI	CCNGG	4 cut(s)	58, 207, 499, 834
SduI	GDGCHC	1 cut(s)	731
SfaNI	GCATC	4 cut(s)	529, 725, 958, 1017
SfcI	CTRYAG	1 cut(s)	597
SinI	GGWCC	1 cut(s)	200
SmiMI	CAYNNNNRTG	1 cut(s)	1035
Sse9I	AATT	8 cut(s)	50, 360, 422, 431, 471, 553, 864, 1090
SsiI	CCGC	3 cut(s)	134, 203, 854
SspMI	CTAG	2 cut(s)	281, 714
SstI	GAGCTC	1 cut(s)	731
StyD4I	CCNGG	4 cut(s)	56, 205, 497, 832
TaaI	ACNGT	7 cut(s)	157, 223, 274, 518, 614, 661, 1003
TaqI	TCGA	3 cut(s)	313, 397, 1128
TaqII	GACCGA	1 cut(s)	1268
TasI	AATT	8 cut(s)	50, 360, 422, 431, 471, 553, 864, 1090
TatI	WGTACW	1 cut(s)	616
TfiI	GAWTC	2 cut(s)	457, 780
Tru1I	TTAA	5 cut(s)	83, 159, 434, 756, 899
Tru9I	TTAA	5 cut(s)	83, 159, 434, 756, 899
TscAI	CASTG	2 cut(s)	876, 1021
TseFI	GTSAC	1 cut(s)	304
TseI	GCWGC	3 cut(s)	494, 596, 971
Tsp45I	GTSAC	1 cut(s)	304
TspDTI	ATGAA	7 cut(s)	242, 258, 848, 899, 917, 1086, 1094
TspGWI	ACGGA	1 cut(s)	938
TspRI	CASTG	2 cut(s)	876, 1021
VpaK11BI	GGWCC	1 cut(s)	200
VspI	ATTAAT	1 cut(s)	756
XapI	RAATTY	2 cut(s)	50, 422
XceI	RCATGY	1 cut(s)	175
XmnI	GAANNNNTTC	1 cut(s)	422
XspI	CTAG	2 cut(s)	281, 714
Zsp2I	ATGCAT	1 cut(s)	1032

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.