Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0009510

Physical Location & Seq

Genomic Coordinates Reverse (-)

66822657 .. 66825871

Gene Structure 3215 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00275350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1323 bp

ATGGCGTCTCTTAGCTTGACTCAGATCAGAGGCAACAACATCAACAACCCAGTTGCTGAGTTTGCCAGAACTCGTTCTCCTCAGTTGGGTTTCCCTCTTCGGTCTAGCAGAGTTGGGTTTCGGGTGTTTGCGTCAGAGAGCAGCAAGGGCCCAGAGCCTGATCTGAGCGTTAATGTAAATGGGTTGCACATGCCCAACCCTTTCGTTATCGGGTCGGGTCCACCCGGTACTAACTACACCGTCATGAAAAGGGCCTTTGATGAAGGCTGGGGTGCTGTGATTGCCAAAACTGTGTCGCTAGACGCAGAAAAAGTGAAGAATGTCACTCCACGATATGCAAAGCTACGAGCTGATGCAAATGGCTCAGCCAAAGGGCAGATTATTGGGTGGGAGAATATAGAACTTATAAGTGATCGACCTCTTGAAACTATGTTGAAAGAATTCAAGCAATTGAAGGAAGAGTATCCGGACAGGATTCTGATTGCTTCAATCATGGAGGAATATGACAAAGCTGGCTGGGAGGAACTAATTGATCGTGTGGAGCAAACTGGAGTTGATGCCTTTGAAATCAACTTCTCCTGTCCTCATGGTATGCCGGAGCGCAAGATGGGTGCTGCTGTTGGGCAAGACTGTGCACTGTTAGAAGAAGTCTGTGGATGGATCAATGCCAAAGCTACAATTCCGGTTTGGGCAAAGATGACTCCTAACGTCACTGACATTACACAGGTCAACTTGCTTTCGCTTACCTTTGCATTACTTCCTAGAGTGGCTCTTAGCTCAGGATGTGAGGGAGTATCAGCTATTAACACAATCATGAGTGTAATGGGAATCAATCTGACTACCTTACGGCCTGAGCCTTGTGTGGAGGGATACTCGACTCCTGGGGGCTATTCTTCCAAGGCAGTTCATCCTATTGCACTTGCAAAAGTGATGAGTATCGCAAAAATGATGAAATCAGAATTTGCTAATAAAGATCTTTCACTTTCTGGGATCGGAGGTGTTGAGACCGGTGGTGATGCTGCTGAATTTATCCTTCTGGGAGCAAATTCTGTTCAGGTCTGTACTGGGGTTATGATGCATGGGTACGGCGTTGTGAAGAAACTCTGTTCTGAGCTAAAGGATTTCATGAGACAGCACAATTTCTCATCTATAGAAGATTTCAGAGGGGCTTCCCTTGAGTATTTTACAACGCATACTGATTTAGTACGCAGACAGCAAGAAGCCATCCTACAAAGAAAAGCTGTGAAGAGAGGGTTGCAGTCCGACAAAGATTGGACAGGAGATGGTTTCGTTAAAGAGACCGAGAGCATGGTCTCTAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003954 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006206 GO:0006208 GO:0006212 GO:0006725 GO:0006807 GO:0007154 GO:0008150 GO:0008152 GO:0009056 GO:0009112 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009987 GO:0009991 GO:0016491 GO:0016627 GO:0016628 GO:0016651 GO:0017113 GO:0017144 GO:0019860 GO:0031667 GO:0031668 GO:0031669 GO:0034641 GO:0042737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043562 GO:0044237 GO:0044238 GO:0044248 GO:0044270 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046113 GO:0046483 GO:0046700 GO:0048037 GO:0050896 GO:0051536 GO:0051540 GO:0051716 GO:0055086 GO:0055114 GO:0071496 GO:0071704 GO:0072527 GO:0072529 GO:1901360 GO:1901361 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00240 ko00410 ko00770 ko00983 ko01100 map00240 map00410 map00770 map00983 map01100

Pfam Domains

Protein Families

DHO_dh

Protein Analysis

440

Amino Acids

48.04

Weight (kDa)

5.99

Isoelectric Point (pI)

43.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHO_dh	PF01180	55 - 376	2.3e-33	Dihydroorotate dehydrogenase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009510)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G17810
fragaria_vesca	FvH4_5g17680 FvH4_5g17680
malus_domestica	MD06G1232500.v1.1 MD14G1239300.v1.1
prunus_persica	Prupe.5G236800_v2.0.a1 Prupe.5G236800_v2.0.a1 Prupe.5G236800_v2.0.a1
pyrus_communis	pycom06g20670
rosa_chinensis	RchiOBHm_Chr7g0177971
rosa_laevigata	RLG00000005486
rosa_multiflora	Rmu_co8250821.1_g000001 Rmu_sc0022313.1_g000001 Rmu_sc0034489.1_g000001
rosa_roxburghii	Rroxscaffold_3G00275350
rosa_rugosa	Rorug06G0411900 Rorug06G0411900
rosa_samantha	Rh7AG010900 Rh7BG010800 Rh7CG011200 Rh7DG010900
rosa_wichuraiana	Rw7G000910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	407
AccIII	TCCGGA	1 cut(s)	466
AclWI	GGATC	2 cut(s)	668, 998
AcsI	RAATTY	4 cut(s)	440, 959, 1025, 1045
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	4 cut(s)	229, 1063, 1085, 1206
AfiI	CCNNNNNNNGG	1 cut(s)	86
AgeI	ACCGGT	1 cut(s)	1007
AgsI	TTSAA	6 cut(s)	425, 436, 445, 454, 489, 566
AjnI	CCWGG	1 cut(s)	880
AloI	GAACNNNNNNTCC	2 cut(s)	61, 93
AluBI	AGCT	9 cut(s)	15, 343, 350, 512, 674, 777, 800, 1114, 1241
AluI	AGCT	9 cut(s)	15, 343, 350, 512, 674, 777, 800, 1114, 1241
Alw21I	GWGCWC	1 cut(s)	637
Alw26I	GTCTC	5 cut(s)	12, 998, 1123, 1292, 1318
Alw44I	GTGCAC	1 cut(s)	633
AlwI	GGATC	2 cut(s)	668, 998
AlwNI	CAGNNNCTG	2 cut(s)	56, 158
Aor13HI	TCCGGA	1 cut(s)	466
AoxI	GGCC	3 cut(s)	148, 252, 848
ApaI	GGGCCC	1 cut(s)	152
ApaLI	GTGCAC	1 cut(s)	633
ApeKI	GCWGC	3 cut(s)	141, 614, 1019
ApoI	RAATTY	4 cut(s)	440, 959, 1025, 1045
AsiGI	ACCGGT	1 cut(s)	1007
Asp700I	GAANNNNTTC	2 cut(s)	73, 440
AspLEI	GCGC	1 cut(s)	603
AspS9I	GGNCC	4 cut(s)	148, 149, 218, 252
AsuC2I	CCSGG	1 cut(s)	225
AsuHPI	GGTGA	1 cut(s)	1025
AvaII	GGWCC	1 cut(s)	218
BaeGI	GKGCMC	2 cut(s)	152, 637
BanII	GRGCYC	1 cut(s)	152
BarI	GAAGNNNNNNTAC	2 cut(s)	1212, 1244
Bbv12I	GWGCWC	1 cut(s)	637
BbvI	GCAGC	3 cut(s)	153, 601, 1006
BccI	CCATC	4 cut(s)	601, 651, 1232, 1277
BceAI	ACGGC	2 cut(s)	863, 1102
BciT130I	CCWGG	1 cut(s)	882
BciVI	GTATCC	2 cut(s)	474, 863
BcnI	CCSGG	1 cut(s)	225
BcoDI	GTCTC	5 cut(s)	12, 998, 1123, 1292, 1318
BfaI	CTAG	3 cut(s)	105, 299, 762
BfmI	CTRYAG	1 cut(s)	1149
BfuI	GTATCC	2 cut(s)	474, 863
BglII	AGATCT	1 cut(s)	973
BisI	GCNGC	3 cut(s)	142, 615, 1020
BlpI	GCTNAGC	1 cut(s)	364
BlsI	GCNGC	3 cut(s)	143, 616, 1021
Bme1390I	CCNGG	2 cut(s)	225, 882
Bme18I	GGWCC	1 cut(s)	218
BmgT120I	GGNCC	4 cut(s)	148, 149, 218, 252
BmiI	GGNNCC	2 cut(s)	150, 219
BmrFI	CCNGG	2 cut(s)	225, 882
BmrI	ACTGGG	2 cut(s)	44, 1074
BmsI	GCATC	4 cut(s)	343, 547, 1006, 1065
BmuI	ACTGGG	2 cut(s)	44, 1074
BplI	GAGNNNNNCTC	2 cut(s)	857, 889
BpmI	CTGGAG	1 cut(s)	570
Bpu10I	CCTNAGC	2 cut(s)	778, 852
Bpu1102I	GCTNAGC	1 cut(s)	364
BpuEI	CTTGAG	1 cut(s)	1196
BpuMI	CCSGG	1 cut(s)	225
BsaBI	GATNNNNATC	1 cut(s)	935
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	3 cut(s)	998, 1292, 1318
BsaJI	CCNNGG	2 cut(s)	881, 897
BsaWI	WCCGGW	3 cut(s)	466, 682, 1007
BsaXI	ACNNNNNCTCC	2 cut(s)	61, 91
Bsc4I	CCNNNNNNNGG	1 cut(s)	86
Bse118I	RCCGGY	1 cut(s)	1007
Bse1I	ACTGG	3 cut(s)	50, 553, 1069
Bse8I	GATNNNNATC	1 cut(s)	935
BseAI	TCCGGA	1 cut(s)	466
BseBI	CCWGG	1 cut(s)	882
BseDI	CCNNGG	2 cut(s)	881, 897
BseGI	GGATG	4 cut(s)	662, 788, 907, 1224
BseJI	GATNNNNATC	1 cut(s)	935
BseLI	CCNNNNNNNGG	1 cut(s)	86
BseMII	CTCAG	8 cut(s)	35, 48, 95, 155, 378, 792, 843, 1101
BseNI	ACTGG	3 cut(s)	50, 553, 1069
BseRI	GAGGAG	1 cut(s)	69
BseSI	GKGCMC	2 cut(s)	152, 637
BseXI	GCAGC	3 cut(s)	153, 601, 1006
BseYI	CCCAGC	2 cut(s)	267, 516
BshFI	GGCC	3 cut(s)	150, 254, 850
BshTI	ACCGGT	1 cut(s)	1007
BsiHKAI	GWGCWC	1 cut(s)	637
BsiSI	CCGG	5 cut(s)	225, 467, 596, 683, 1008
BslI	CCNNNNNNNGG	1 cut(s)	86
BsmAI	GTCTC	5 cut(s)	12, 998, 1123, 1292, 1318
BsmBI	CGTCTC	1 cut(s)	12
BsnI	GGCC	3 cut(s)	150, 254, 850
Bso31I	GGTCTC	3 cut(s)	998, 1292, 1318
Bsp120I	GGGCCC	1 cut(s)	148
Bsp1286I	GDGCHC	2 cut(s)	152, 637
Bsp13I	TCCGGA	1 cut(s)	466
Bsp143I	GATC	7 cut(s)	24, 160, 412, 532, 660, 973, 990
Bsp1720I	GCTNAGC	1 cut(s)	364
BspANI	GGCC	3 cut(s)	150, 254, 850
BspCNI	CTCAG	8 cut(s)	34, 49, 94, 156, 377, 791, 844, 1102
BspEI	TCCGGA	1 cut(s)	466
BspHI	TCATGA	3 cut(s)	243, 813, 1125
BspLI	GGNNCC	2 cut(s)	150, 219
BspPI	GGATC	2 cut(s)	668, 998
BspTNI	GGTCTC	3 cut(s)	998, 1292, 1318
BsrFI	RCCGGY	1 cut(s)	1007
BsrI	ACTGG	3 cut(s)	50, 553, 1069
BssAI	RCCGGY	1 cut(s)	1007
BssECI	CCNNGG	2 cut(s)	881, 897
BssMI	GATC	7 cut(s)	24, 160, 412, 532, 660, 973, 990
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	1 cut(s)	897
Bst2UI	CCWGG	1 cut(s)	882
Bst4CI	ACNGT	4 cut(s)	241, 292, 632, 639
Bst6I	CTCTTC	3 cut(s)	102, 453, 1241
BstACI	GRCGYC	1 cut(s)	5
BstC8I	GCNNGC	1 cut(s)	514
BstF5I	GGATG	4 cut(s)	662, 788, 907, 1224
BstHHI	GCGC	1 cut(s)	603
BstKTI	GATC	7 cut(s)	27, 163, 415, 535, 663, 976, 993
BstMAI	GTCTC	5 cut(s)	12, 998, 1123, 1292, 1318
BstMBI	GATC	7 cut(s)	24, 160, 412, 532, 660, 973, 990
BstMWI	GCNNNNNNNGC	3 cut(s)	62, 147, 281
BstNI	CCWGG	1 cut(s)	882
BstNSI	RCATGY	1 cut(s)	193
BstSCI	CCNGG	2 cut(s)	223, 880
BstSFI	CTRYAG	1 cut(s)	1149
BstSLI	GKGCMC	2 cut(s)	152, 637
BstV1I	GCAGC	3 cut(s)	153, 601, 1006
BstX2I	RGATCY	1 cut(s)	973
BstYI	RGATCY	1 cut(s)	973
BsuI	GTATCC	2 cut(s)	474, 863
BsuRI	GGCC	3 cut(s)	150, 254, 850
BtsCI	GGATG	4 cut(s)	662, 788, 907, 1224
BtsIMutI	CAGTG	2 cut(s)	635, 711
Cac8I	GCNNGC	1 cut(s)	514
CaiI	CAGNNNCTG	2 cut(s)	56, 158
CciI	TCATGA	3 cut(s)	243, 813, 1125
CfoI	GCGC	1 cut(s)	603
Cfr10I	RCCGGY	1 cut(s)	1007
Cfr13I	GGNCC	4 cut(s)	148, 149, 218, 252
CseI	GACGC	2 cut(s)	120, 311
Csp6I	GTAC	4 cut(s)	228, 1062, 1084, 1205
CspAI	ACCGGT	1 cut(s)	1007
CviAII	CATG	8 cut(s)	190, 244, 493, 587, 814, 1079, 1126, 1309
CviQI	GTAC	4 cut(s)	228, 1062, 1084, 1205
DpnI	GATC	7 cut(s)	26, 162, 414, 534, 662, 975, 992
DpnII	GATC	7 cut(s)	24, 160, 412, 532, 660, 973, 990
Eam1104I	CTCTTC	3 cut(s)	102, 453, 1241
EarI	CTCTTC	3 cut(s)	102, 453, 1241
Eco130I	CCWWGG	1 cut(s)	897
Eco24I	GRGCYC	1 cut(s)	152
Eco31I	GGTCTC	3 cut(s)	998, 1292, 1318
Eco47I	GGWCC	1 cut(s)	218
EcoO109I	RGGNCCY	2 cut(s)	148, 252
EcoRI	GAATTC	1 cut(s)	440
EcoRII	CCWGG	1 cut(s)	880
EcoT14I	CCWWGG	1 cut(s)	897
EcoT22I	ATGCAT	1 cut(s)	1080
EcoT38I	GRGCYC	1 cut(s)	152
ErhI	CCWWGG	1 cut(s)	897
Esp3I	CGTCTC	1 cut(s)	12
FaeI	CATG	8 cut(s)	193, 247, 496, 590, 817, 1082, 1129, 1312
FatI	CATG	8 cut(s)	189, 243, 492, 586, 813, 1078, 1125, 1308
Fnu4HI	GCNGC	3 cut(s)	142, 615, 1020
FokI	GGATG	4 cut(s)	669, 795, 894, 1211
FriOI	GRGCYC	1 cut(s)	152
Fsp4HI	GCNGC	3 cut(s)	142, 615, 1020
FspBI	CTAG	3 cut(s)	105, 299, 762
GlaI	GCGC	1 cut(s)	602
GluI	GCNGC	3 cut(s)	142, 615, 1020
GsaI	CCCAGC	2 cut(s)	271, 520
GsuI	CTGGAG	1 cut(s)	570
HaeIII	GGCC	3 cut(s)	150, 254, 850
HapII	CCGG	5 cut(s)	225, 467, 596, 683, 1008
HgaI	GACGC	2 cut(s)	120, 311
HhaI	GCGC	1 cut(s)	603
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	8 cut(s)	193, 247, 496, 590, 817, 1082, 1129, 1312
Hin6I	GCGC	1 cut(s)	601
HinP1I	GCGC	1 cut(s)	601
HincII	GTYRAC	1 cut(s)	730
HindII	GTYRAC	1 cut(s)	730
HinfI	GANTC	5 cut(s)	19, 475, 700, 828, 877
HpaII	CCGG	5 cut(s)	225, 467, 596, 683, 1008
HphI	GGTGA	1 cut(s)	1025
Hpy166II	GTNNAC	3 cut(s)	221, 635, 730
Hpy188III	TCNNGA	6 cut(s)	244, 422, 467, 780, 814, 1126
Hpy8I	GTNNAC	3 cut(s)	221, 635, 730
HpyAV	CCTTC	3 cut(s)	257, 448, 1043
HpyCH4III	ACNGT	4 cut(s)	241, 292, 632, 639
HpyCH4IV	ACGT	1 cut(s)	708
HpyCH4V	TGCA	9 cut(s)	187, 338, 356, 635, 752, 917, 923, 1078, 1258
HpyF10VI	GCNNNNNNNGC	3 cut(s)	62, 147, 281
HpySE526I	ACGT	1 cut(s)	708
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	8 cut(s)	193, 247, 496, 590, 817, 1082, 1129, 1312
HspAI	GCGC	1 cut(s)	601
Kpn2I	TCCGGA	1 cut(s)	466
Kzo9I	GATC	7 cut(s)	24, 160, 412, 532, 660, 973, 990
LmnI	GCTCC	3 cut(s)	541, 598, 1040
Lsp1109I	GCAGC	3 cut(s)	153, 601, 1006
LweI	GCATC	4 cut(s)	343, 547, 1006, 1065
MaeI	CTAG	3 cut(s)	105, 299, 762
MaeII	ACGT	1 cut(s)	708
MaeIII	GTNAC	2 cut(s)	322, 709
MalI	GATC	7 cut(s)	26, 162, 414, 534, 662, 975, 992
MboI	GATC	7 cut(s)	24, 160, 412, 532, 660, 973, 990
MboII	GAAGA	8 cut(s)	89, 328, 470, 656, 885, 1108, 1166, 1258
MfeI	CAATTG	1 cut(s)	449
MflI	RGATCY	1 cut(s)	973
MhlI	GDGCHC	2 cut(s)	152, 637
MluCI	AATT	9 cut(s)	440, 449, 528, 678, 959, 1025, 1045, 1138, 1318
MlyI	GAGTC	3 cut(s)	13, 694, 871
MmeI	TCCRAC	1 cut(s)	1287
Mph1103I	ATGCAT	1 cut(s)	1080
MroI	TCCGGA	1 cut(s)	466
MroXI	GAANNNNTTC	2 cut(s)	73, 440
MseI	TTAA	4 cut(s)	171, 804, 1293, 1321
MslI	CAYNNNNRTG	2 cut(s)	242, 812
MspI	CCGG	5 cut(s)	225, 467, 596, 683, 1008
MspR9I	CCNGG	2 cut(s)	225, 882
MunI	CAATTG	1 cut(s)	449
MvaI	CCWGG	1 cut(s)	882
MwoI	GCNNNNNNNGC	3 cut(s)	62, 147, 281
NciI	CCSGG	1 cut(s)	225
NdeII	GATC	7 cut(s)	24, 160, 412, 532, 660, 973, 990
NlaIII	CATG	8 cut(s)	193, 247, 496, 590, 817, 1082, 1129, 1312
NlaIV	GGNNCC	2 cut(s)	150, 219
NmuCI	GTSAC	2 cut(s)	322, 709
NsiI	ATGCAT	1 cut(s)	1080
NspI	RCATGY	1 cut(s)	193
PagI	TCATGA	3 cut(s)	243, 813, 1125
PdmI	GAANNNNTTC	2 cut(s)	73, 440
PfeI	GAWTC	2 cut(s)	475, 828
PinAI	ACCGGT	1 cut(s)	1007
PkrI	GCNGC	3 cut(s)	143, 616, 1021
PleI	GAGTC	3 cut(s)	13, 694, 871
PpsI	GAGTC	3 cut(s)	13, 694, 871
PsiI	TTATAA	1 cut(s)	407
Psp6I	CCWGG	1 cut(s)	880
PspFI	CCCAGC	2 cut(s)	267, 516
PspGI	CCWGG	1 cut(s)	880
PspN4I	GGNNCC	2 cut(s)	150, 219
PspOMI	GGGCCC	1 cut(s)	148
PspPI	GGNCC	4 cut(s)	148, 149, 218, 252
PstNI	CAGNNNCTG	2 cut(s)	56, 158
PsuI	RGATCY	1 cut(s)	973
RsaI	GTAC	4 cut(s)	229, 1063, 1085, 1206
RsaNI	GTAC	4 cut(s)	228, 1062, 1084, 1205
RseI	CAYNNNNRTG	2 cut(s)	242, 812
SaqAI	TTAA	4 cut(s)	171, 804, 1293, 1321
SatI	GCNGC	3 cut(s)	142, 615, 1020
Sau3AI	GATC	7 cut(s)	24, 160, 412, 532, 660, 973, 990
Sau96I	GGNCC	4 cut(s)	148, 149, 218, 252
SchI	GAGTC	3 cut(s)	13, 694, 871
ScrFI	CCNGG	2 cut(s)	225, 882
SduI	GDGCHC	2 cut(s)	152, 637
SfaNI	GCATC	4 cut(s)	343, 547, 1006, 1065
SfcI	CTRYAG	1 cut(s)	1149
SinI	GGWCC	1 cut(s)	218
SmiMI	CAYNNNNRTG	2 cut(s)	242, 812
SmlI	CTYRAG	1 cut(s)	1175
SmoI	CTYRAG	1 cut(s)	1175
Sse9I	AATT	9 cut(s)	440, 449, 528, 678, 959, 1025, 1045, 1138, 1318
SspMI	CTAG	3 cut(s)	105, 299, 762
StyD4I	CCNGG	2 cut(s)	223, 880
StyI	CCWWGG	1 cut(s)	897
TaaI	ACNGT	4 cut(s)	241, 292, 632, 639
TaiI	ACGT	1 cut(s)	711
TaqI	TCGA	2 cut(s)	415, 875
TaqII	GACCGA	2 cut(s)	90, 1316
TasI	AATT	9 cut(s)	440, 449, 528, 678, 959, 1025, 1045, 1138, 1318
TatI	WGTACW	1 cut(s)	1061
TfiI	GAWTC	2 cut(s)	475, 828
Tru1I	TTAA	4 cut(s)	171, 804, 1293, 1321
Tru9I	TTAA	4 cut(s)	171, 804, 1293, 1321
TscAI	CASTG	2 cut(s)	642, 718
TseFI	GTSAC	2 cut(s)	322, 709
TseI	GCWGC	3 cut(s)	141, 614, 1019
Tsp45I	GTSAC	2 cut(s)	322, 709
TspDTI	ATGAA	5 cut(s)	260, 276, 896, 965, 1114
TspRI	CASTG	2 cut(s)	642, 718
VneI	GTGCAC	1 cut(s)	633
VpaK11BI	GGWCC	1 cut(s)	218
XapI	RAATTY	4 cut(s)	440, 959, 1025, 1045
XceI	RCATGY	1 cut(s)	193
XmnI	GAANNNNTTC	2 cut(s)	73, 440
XspI	CTAG	3 cut(s)	105, 299, 762
Zsp2I	ATGCAT	1 cut(s)	1080

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.