MD15G1032300.v1.1

Sin3 binding region of histone deacetylase complex subunit SAP30

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0008660
Physical Location & Seq
Forward (+)
1996626 .. 2000294
3669 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1032300.v1.1.491

Sequence Viewer

Length: 678 bp
ATGCTCGAGGCAATGGAGAGCTCCGTCAATGGGGGCGGATTCTCGCACTTGCAGAGTTGCGGGGACAGCAGCGAGGAGGAGCTATCGGTGCTTCCCCGCCACACAAAGGTGGTGGTCACGGGCAATAACCGTACCAAGTCGGTGCTTGTTGGCCTCCAGGGCGTCGTCAAGAAGGCCGTCGGGCTCGGCGGGTGGCATTGGCTGGTTCTCACAAACGGCATAGAAGTGAAGCTTCAGAGGAATGCCCTTAGCGTGATTGAAGCTCCAACTGGTAATGAGGAAGATGACGACCTTGATTTCGAGAATGTGCACTGGAATGGATCAGATATGGCATCCGATGACACTCAAAAGTCCCACAGATCAAGGCACAGGGTGCATAAATCATCTGGATCATCTCACAAGACTATGTGCAGAACTTTCTCTTGTGATTCACAATCTAAGGGTTCTATTTGTACTCCCCGAGGGTCCACGAAAATTGACCTTGGGAAACTGGAGATGGCTGCTTTATGGAGATATTGGCGACACTTCAATCTTGTGGACGCCATCCCTAACCCATCGAAAGAGGAACTAATTGATGTTGTTCAAAGGCATTTCATGTCACAGCCAATCGACGAGTTGCAGGTCATTGTGGGGTTTGTTCAGGCTGCGAGGAAACTAAAGAAGACTGTTCGCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SAP30_Sin3_bdg

Protein Analysis

226

Amino Acids

25.0

Weight (kDa)

8.34

Isoelectric Point (pI)

49.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SAP30_Sin3_bdg PF13867 164 - 217 1.5e-20 Sin3 binding region of histone deacetylase complex subunit SAP30
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 610
AciI CCGC 4 cut(s) 36, 60, 97, 189
AclWI GGATC 2 cut(s) 328, 397
AcuI CTGAAG 1 cut(s) 218
AcyI GRCGYC 2 cut(s) 162, 540
AdeI CACNNNGTG 1 cut(s) 373
AfaI GTAC 2 cut(s) 133, 454
AfiI CCNNNNNNNGG 2 cut(s) 30, 106
AgsI TTSAA 3 cut(s) 260, 529, 584
AjnI CCWGG 1 cut(s) 156
AleI CACNNNNGTG 1 cut(s) 107
AluBI AGCT 4 cut(s) 21, 82, 232, 263
AluI AGCT 4 cut(s) 21, 82, 232, 263
Alw21I GWGCWC 2 cut(s) 23, 312
Alw44I GTGCAC 1 cut(s) 308
AlwI GGATC 2 cut(s) 328, 397
Ama87I CYCGRG 2 cut(s) 5, 459
AoxI GGCC 2 cut(s) 151, 174
ApaLI GTGCAC 1 cut(s) 308
ApeKI GCWGC 3 cut(s) 69, 500, 644
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
AspS9I GGNCC 1 cut(s) 465
AvaI CYCGRG 2 cut(s) 5, 459
AvaII GGWCC 1 cut(s) 465
BaeGI GKGCMC 1 cut(s) 312
BanII GRGCYC 2 cut(s) 23, 186
BbsI GAAGAC 1 cut(s) 668
Bbv12I GWGCWC 2 cut(s) 23, 312
BbvI GCAGC 3 cut(s) 81, 487, 631
BccI CCATC 3 cut(s) 490, 551, 562
BceAI ACGGC 2 cut(s) 161, 232
BciT130I CCWGG 1 cut(s) 158
BfuAI ACCTGC 1 cut(s) 610
BglI GCCNNNNNGGC 1 cut(s) 159
BisI GCNGC 3 cut(s) 70, 501, 645
BlsI GCNGC 3 cut(s) 71, 502, 646
Bme1390I CCNGG 1 cut(s) 158
Bme18I GGWCC 1 cut(s) 465
BmeT110I CYCGRG 2 cut(s) 5, 459
BmgT120I GGNCC 1 cut(s) 465
BmiI GGNNCC 1 cut(s) 466
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 1 cut(s) 341
BpiI GAAGAC 1 cut(s) 668
BpmI CTGGAG 2 cut(s) 140, 512
Bpu10I CCTNAGC 1 cut(s) 248
BsaHI GRCGYC 2 cut(s) 162, 540
BsaJI CCNNGG 3 cut(s) 157, 460, 481
Bsc4I CCNNNNNNNGG 2 cut(s) 30, 106
Bse1I ACTGG 3 cut(s) 274, 317, 495
Bse3DI GCAATG 1 cut(s) 18
BseBI CCWGG 1 cut(s) 158
BseDI CCNNGG 3 cut(s) 157, 460, 481
BseGI GGATG 2 cut(s) 332, 543
BseLI CCNNNNNNNGG 2 cut(s) 30, 106
BseMI GCAATG 1 cut(s) 18
BseNI ACTGG 3 cut(s) 274, 317, 495
BseRI GAGGAG 2 cut(s) 89, 92
BseSI GKGCMC 1 cut(s) 312
BseXI GCAGC 3 cut(s) 81, 487, 631
BsgI GTGCAG 1 cut(s) 430
BshFI GGCC 2 cut(s) 153, 176
BsiHKAI GWGCWC 2 cut(s) 23, 312
BsiHKCI CYCGRG 2 cut(s) 5, 459
BslFI GGGAC 2 cut(s) 77, 337
BslI CCNNNNNNNGG 2 cut(s) 30, 106
BsmFI GGGAC 2 cut(s) 77, 337
BsmI GAATGC 1 cut(s) 247
BsnI GGCC 2 cut(s) 153, 176
BsoBI CYCGRG 2 cut(s) 5, 459
Bsp1286I GDGCHC 3 cut(s) 23, 186, 312
Bsp143I GATC 3 cut(s) 320, 359, 389
BspACI CCGC 4 cut(s) 36, 60, 97, 189
BspANI GGCC 2 cut(s) 153, 176
BspLI GGNNCC 1 cut(s) 466
BspMI ACCTGC 1 cut(s) 610
BspPI GGATC 2 cut(s) 328, 397
BsrDI GCAATG 1 cut(s) 18
BsrI ACTGG 3 cut(s) 274, 317, 495
BssECI CCNNGG 3 cut(s) 157, 460, 481
BssMI GATC 3 cut(s) 320, 359, 389
BssNI GRCGYC 2 cut(s) 162, 540
BssT1I CCWWGG 1 cut(s) 481
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 2 cut(s) 131, 667
BstACI GRCGYC 2 cut(s) 162, 540
BstAPI GCANNNNNTGC 1 cut(s) 373
BstDEI CTNAG 2 cut(s) 248, 438
BstF5I GGATG 2 cut(s) 332, 543
BstKTI GATC 3 cut(s) 323, 362, 392
BstMBI GATC 3 cut(s) 320, 359, 389
BstMWI GCNNNNNNNGC 4 cut(s) 66, 88, 159, 373
BstNI CCWGG 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 156
BstSLI GKGCMC 1 cut(s) 312
BstV1I GCAGC 3 cut(s) 81, 487, 631
BstV2I GAAGAC 1 cut(s) 668
BsuRI GGCC 2 cut(s) 153, 176
BtsCI GGATG 2 cut(s) 332, 543
BtsIMutI CAGTG 1 cut(s) 310
BveI ACCTGC 1 cut(s) 610
Cfr13I GGNCC 1 cut(s) 465
CseI GACGC 2 cut(s) 151, 548
Csp6I GTAC 2 cut(s) 132, 453
CspCI CAANNNNNGTGG 4 cut(s) 93, 128, 457, 492
CviAII CATG 1 cut(s) 595
CviQI GTAC 2 cut(s) 132, 453
DdeI CTNAG 2 cut(s) 248, 438
DpnI GATC 3 cut(s) 322, 361, 391
DpnII GATC 3 cut(s) 320, 359, 389
DraIII CACNNNGTG 1 cut(s) 373
EciI GGCGGA 1 cut(s) 51
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 1 cut(s) 481
Eco24I GRGCYC 2 cut(s) 23, 186
Eco47I GGWCC 1 cut(s) 465
Eco53kI GAGCTC 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 218
Eco88I CYCGRG 2 cut(s) 5, 459
EcoICRI GAGCTC 1 cut(s) 21
EcoRII CCWGG 1 cut(s) 156
EcoT14I CCWWGG 1 cut(s) 481
EcoT38I GRGCYC 2 cut(s) 23, 186
ErhI CCWWGG 1 cut(s) 481
FaeI CATG 1 cut(s) 598
FaiI YATR 6 cut(s) 221, 329, 378, 407, 508, 596
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FaqI GGGAC 2 cut(s) 77, 337
FatI CATG 1 cut(s) 594
FauI CCCGC 3 cut(s) 53, 104, 182
Fnu4HI GCNGC 3 cut(s) 70, 501, 645
FokI GGATG 2 cut(s) 319, 530
FriOI GRGCYC 2 cut(s) 23, 186
Fsp4HI GCNGC 3 cut(s) 70, 501, 645
GluI GCNGC 3 cut(s) 70, 501, 645
GsuI CTGGAG 2 cut(s) 140, 512
HaeIII GGCC 2 cut(s) 153, 176
HgaI GACGC 2 cut(s) 151, 548
Hin1I GRCGYC 2 cut(s) 162, 540
Hin1II CATG 1 cut(s) 598
HindIII AAGCTT 1 cut(s) 230
HinfI GANTC 2 cut(s) 39, 428
Hpy166II GTNNAC 3 cut(s) 310, 468, 538
Hpy188I TCNGA 3 cut(s) 237, 325, 337
Hpy188III TCNNGA 3 cut(s) 169, 301, 387
Hpy8I GTNNAC 3 cut(s) 310, 468, 538
Hpy99I CGWCG 3 cut(s) 167, 182, 614
HpyAV CCTTC 1 cut(s) 166
HpyCH4III ACNGT 2 cut(s) 131, 667
HpyCH4V TGCA 5 cut(s) 52, 310, 376, 411, 619
HpyF10VI GCNNNNNNNGC 4 cut(s) 66, 88, 159, 373
HpyF3I CTNAG 2 cut(s) 248, 438
Hsp92I GRCGYC 2 cut(s) 162, 540
Hsp92II CATG 1 cut(s) 598
Kzo9I GATC 3 cut(s) 320, 359, 389
LmnI GCTCC 3 cut(s) 26, 79, 268
Lsp1109I GCAGC 3 cut(s) 81, 487, 631
LweI GCATC 1 cut(s) 341
MaeIII GTNAC 2 cut(s) 115, 597
MalI GATC 3 cut(s) 322, 361, 391
MboI GATC 3 cut(s) 320, 359, 389
MboII GAAGA 2 cut(s) 293, 673
MhlI GDGCHC 3 cut(s) 23, 186, 312
MluCI AATT 2 cut(s) 474, 570
MmeI TCCRAC 1 cut(s) 290
MnlI CCTC 8 cut(s) 67, 70, 164, 231, 271, 455, 556, 642
MslI CAYNNNNRTG 3 cut(s) 107, 224, 315
MspR9I CCNGG 1 cut(s) 158
Mva1269I GAATGC 1 cut(s) 247
MvaI CCWGG 1 cut(s) 158
MwoI GCNNNNNNNGC 4 cut(s) 66, 88, 159, 373
NdeII GATC 3 cut(s) 320, 359, 389
NlaIII CATG 1 cut(s) 598
NlaIV GGNNCC 1 cut(s) 466
NmeAIII GCCGAG 1 cut(s) 165
NmuCI GTSAC 2 cut(s) 115, 597
OliI CACNNNNGTG 1 cut(s) 107
PaeR7I CTCGAG 1 cut(s) 5
PctI GAATGC 1 cut(s) 247
PfeI GAWTC 2 cut(s) 39, 428
PkrI GCNGC 3 cut(s) 71, 502, 646
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PspN4I GGNNCC 1 cut(s) 466
PspPI GGNCC 1 cut(s) 465
PspXI VCTCGAGB 1 cut(s) 5
RsaI GTAC 2 cut(s) 133, 454
RsaNI GTAC 2 cut(s) 132, 453
RseI CAYNNNNRTG 3 cut(s) 107, 224, 315
SacI GAGCTC 1 cut(s) 23
SatI GCNGC 3 cut(s) 70, 501, 645
Sau3AI GATC 3 cut(s) 320, 359, 389
Sau96I GGNCC 1 cut(s) 465
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 3 cut(s) 23, 186, 312
SetI ASST 8 cut(s) 23, 84, 111, 234, 265, 294, 483, 624
SfaNI GCATC 1 cut(s) 341
Sfr274I CTCGAG 1 cut(s) 5
SinI GGWCC 1 cut(s) 465
SlaI CTCGAG 1 cut(s) 5
SmiMI CAYNNNNRTG 3 cut(s) 107, 224, 315
SmlI CTYRAG 1 cut(s) 5
SmoI CTYRAG 1 cut(s) 5
Sse9I AATT 2 cut(s) 474, 570
SsiI CCGC 4 cut(s) 36, 60, 97, 189
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 156
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 2 cut(s) 131, 667
TaqI TCGA 4 cut(s) 6, 300, 557, 609
TasI AATT 2 cut(s) 474, 570
TatI WGTACW 1 cut(s) 452
TfiI GAWTC 2 cut(s) 39, 428
TscAI CASTG 1 cut(s) 317
TseFI GTSAC 2 cut(s) 115, 597
TseI GCWGC 3 cut(s) 69, 500, 644
Tsp45I GTSAC 2 cut(s) 115, 597
TspDTI ATGAA 1 cut(s) 583
TspGWI ACGGA 1 cut(s) 13
TspRI CASTG 1 cut(s) 317
VneI GTGCAC 1 cut(s) 308
VpaK11BI GGWCC 1 cut(s) 465
XhoI CTCGAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.