MD15G1058000.v1.1

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009657
Physical Location & Seq
Forward (+)
3895370 .. 3898712
3343 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1058000.v1.1.491

Sequence Viewer

Length: 1602 bp
ATGATGAATAAAGGTTTGATGGTGGATGAGAATATGTCAAATCTGACTTGTGCTTCTGGTGATTTAAGTGCTTCTAACTCAAGCATCAGAAATGAGTCATCTTCTGCTGGCACAGTCTACCCTCATCCCGCATCCTCAGCACAAATTCAGCAGCAGCAAGCACCGCCACCGCCAAAGAAGAAGAGAAATCTCCCAGGCAACCCAGACCCGGATGCTGAAGTAATAGCCTTGTCTCCAAAATCACTGATGGCAACGAATAGATTCGTGTGTGAGATCTGCAACAAAGGGTTTCAAAGAGAGCAAAACCTTCAGCTTCATAGAAGAGGGCACAATCTGCCATGGAAGCTAAAGCAAAGGACGGGCAAGGAGGTGAGGAAGAAGGTGTATCTGTGCCCGGAACCAACATGTGTCCACCATGAACCATCAAGGGCTCTTGGGGACTTGACTGGGATCAAGAAGCACTTCTCTAGAAAGCACGGTGAGAAAAAGTGGAAATGTGAGAAATGCTCAAAGCGGTATGCAGTTCAGTCGGACTGGAAAGCTCACTCCAAAGTCTGTGGCACAAGGGAGTATAGATGTGATTGTGGAACCCTTTTCTACAGGAGGGACAGTTTCATCACACACAGAGCCTTTTGTGATGCTTTAGCACAAGAGAACAATACAAGCTCTGCAGCTGCCAGATCGGTAATGCCAGCCTCAATAAACCCACTTCTCTCCTCACTCCCCCAACTCCATAGCCATGGCCTCCAAGTTCAATCAGCTGTCAAGAGAGAACAGGATCAGCATCAGCAGCAGCTCCTTCCTCCGTGGCTTTCATTCTTGCCAGAAGGCGGCGAAGCGGCCAGCCTCCCTACTATGAACCTCTCCGCCTCGCCATTATTCTCCACCTCTTCATTCCAGCAATATTATTCTTTTGACCAAAACCCTAACCCTAGCAGCTCTTCTACTGCCCTACTCCCTGATAACTTCCAACTTCAACAAAACACCGCTTCCCCTCACATGTCAGCCACTGCCTTGCTTCAGAAGGCATCGGAAATGGGTGCGACCATCAGCAAAACCTCCCCTTCCCCATATTTTCTCAAACCACCCACCCACCAATCCTACCTAGCTCACCAAGCTCACATGTCCAACGAAACTTCGCGTGACCAAGCAATACTATTGGATGATGGGTTTGGAAACAATGAGAGTAAATCTTCACTCTTCCATGATATGATGATGATGAGTAGCTCTCATGGTTTTGGTCATGATCATCATCAAGTCTCGTCGTCGTCGTTTGGAGATCATCAGCAGCAGCAGCAGCAGGCTTTCAATGGAATTATGGTCGACGGTAATTTCGTCGAGATTAATAATAATAATCCTCCAAATAATTATAATAAACCGAGAAGTACTGACAACAACAACAACAACGAGGGTTTGACGAGAGATTTCTTGGGACTTAGAGCGCCATTTTCATCAGCAGCAGCATCGCATGGCGGCCACGGTGATTTATTCAGTATTGATATGGCGGGGCTCGACCATCACCACCATGTGAGTACTTCTTCTCCAGCTGCTTATAACAATGGACAGCAAAATGATCAGAACCAAAAATCGTGGCAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2 zf-C2H2_jaz

Protein Analysis

534

Amino Acids

58.98

Weight (kDa)

8.54

Isoelectric Point (pI)

51.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 125 - 159 1.3e-22 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 163 - 187 1.5e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 190 - 225 1.9e-21 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1371, 1554
AccI GTMKAC 2 cut(s) 117, 1323
AccII CGCG 1 cut(s) 1141
AclWI GGATC 2 cut(s) 458, 786
AcoI YGGCCR 2 cut(s) 840, 1474
AcsI RAATTY 1 cut(s) 144
AcuI CTGAAG 3 cut(s) 237, 293, 1004
AdeI CACNNNGTG 1 cut(s) 1528
AfaI GTAC 2 cut(s) 1387, 1534
AfiI CCNNNNNNNGG 2 cut(s) 208, 830
AflIII ACRYGT 3 cut(s) 404, 999, 1122
AgsI TTSAA 4 cut(s) 293, 755, 977, 1309
AjnI CCWGG 1 cut(s) 193
Alw26I GTCTC 2 cut(s) 237, 1264
AlwI GGATC 2 cut(s) 458, 786
AlwNI CAGNNNCTG 1 cut(s) 1010
AoxI GGCC 3 cut(s) 742, 840, 1474
ApoI RAATTY 1 cut(s) 144
ArsI GACNNNNNNTTYG 2 cut(s) 1316, 1348
AseI ATTAAT 1 cut(s) 1344
Asp700I GAANNNNTTC 2 cut(s) 260, 461
AspLEI GCGC 1 cut(s) 1444
AsuC2I CCSGG 2 cut(s) 209, 395
AsuHPI GGTGA 6 cut(s) 71, 382, 491, 1103, 1493, 1511
BaeGI GKGCMC 2 cut(s) 330, 395
BanII GRGCYC 2 cut(s) 433, 1512
BbvCI CCTCAGC 1 cut(s) 136
BccI CCATC 6 cut(s) 13, 241, 430, 1055, 1160, 1524
BcgI CGANNNNNNTGC 4 cut(s) 510, 544, 1446, 1480
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 2 cut(s) 1246, 1573
BcnI CCSGG 2 cut(s) 209, 395
BcoDI GTCTC 2 cut(s) 237, 1264
BfaI CTAG 3 cut(s) 468, 933, 1106
BfmI CTRYAG 2 cut(s) 598, 669
BfoI RGCGCY 1 cut(s) 1445
BglII AGATCT 1 cut(s) 273
BmcAI AGTACT 2 cut(s) 1387, 1534
Bme1390I CCNGG 3 cut(s) 195, 209, 395
BmiI GGNNCC 2 cut(s) 399, 589
BmrFI CCNGG 3 cut(s) 195, 209, 395
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 7 cut(s) 93, 140, 202, 628, 793, 1037, 1472
BmuI ACTGGG 1 cut(s) 456
BplI GAGNNNNNCTC 4 cut(s) 491, 523, 1213, 1245
BpmI CTGGAG 1 cut(s) 1527
Bpu10I CCTNAGC 1 cut(s) 136
BpuEI CTTGAG 1 cut(s) 64
BpuMI CCSGG 2 cut(s) 209, 395
BsaBI GATNNNNATC 2 cut(s) 783, 1251
BsaJI CCNNGG 5 cut(s) 193, 338, 739, 806, 1477
BsaXI ACNNNNNCTCC 4 cut(s) 595, 625, 1525, 1555
Bsc4I CCNNNNNNNGG 2 cut(s) 208, 830
Bse1I ACTGG 2 cut(s) 451, 539
Bse8I GATNNNNATC 2 cut(s) 783, 1251
BseBI CCWGG 1 cut(s) 195
BseDI CCNNGG 5 cut(s) 193, 338, 739, 806, 1477
BseGI GGATG 5 cut(s) 31, 124, 131, 217, 1168
BseJI GATNNNNATC 2 cut(s) 783, 1251
BseLI CCNNNNNNNGG 2 cut(s) 208, 830
BseMII CTCAG 1 cut(s) 150
BseNI ACTGG 2 cut(s) 451, 539
BseRI GAGGAG 1 cut(s) 706
BseSI GKGCMC 2 cut(s) 330, 395
Bsh1236I CGCG 1 cut(s) 1141
BshFI GGCC 3 cut(s) 744, 842, 1476
BsiSI CCGG 2 cut(s) 209, 395
BslFI GGGAC 3 cut(s) 452, 620, 1446
BslI CCNNNNNNNGG 2 cut(s) 208, 830
BsmAI GTCTC 2 cut(s) 237, 1264
BsmFI GGGAC 3 cut(s) 452, 620, 1446
BsnI GGCC 3 cut(s) 744, 842, 1476
Bsp1286I GDGCHC 4 cut(s) 330, 395, 433, 1512
Bsp143I GATC 7 cut(s) 273, 450, 680, 778, 1246, 1279, 1573
Bsp19I CCATGG 2 cut(s) 338, 739
BspANI GGCC 3 cut(s) 744, 842, 1476
BspCNI CTCAG 1 cut(s) 149
BspFNI CGCG 1 cut(s) 1141
BspHI TCATGA 1 cut(s) 1243
BspLI GGNNCC 2 cut(s) 399, 589
BspMAI CTGCAG 1 cut(s) 673
BspPI GGATC 2 cut(s) 458, 786
BspQI GCTCTTC 1 cut(s) 946
BsrI ACTGG 2 cut(s) 451, 539
BssECI CCNNGG 5 cut(s) 193, 338, 739, 806, 1477
BssMI GATC 7 cut(s) 273, 450, 680, 778, 1246, 1279, 1573
BssT1I CCWWGG 2 cut(s) 338, 739
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 5 cut(s) 115, 479, 611, 1328, 1481
Bst6I CTCTTC 5 cut(s) 176, 316, 895, 946, 1205
BstAPI GCANNNNNTGC 1 cut(s) 334
BstC8I GCNNGC 5 cut(s) 109, 159, 693, 844, 1302
BstDEI CTNAG 2 cut(s) 136, 1436
BstDSI CCRYGG 4 cut(s) 338, 739, 806, 1477
BstF5I GGATG 5 cut(s) 31, 124, 131, 217, 1168
BstFNI CGCG 1 cut(s) 1141
BstH2I RGCGCY 1 cut(s) 1445
BstHHI GCGC 1 cut(s) 1444
BstKTI GATC 7 cut(s) 276, 453, 683, 781, 1249, 1282, 1576
BstMAI GTCTC 2 cut(s) 237, 1264
BstMBI GATC 7 cut(s) 273, 450, 680, 778, 1246, 1279, 1573
BstMWI GCNNNNNNNGC 8 cut(s) 137, 163, 334, 343, 790, 1115, 1294, 1297
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 3 cut(s) 408, 1003, 1126
BstSCI CCNGG 3 cut(s) 193, 207, 393
BstSFI CTRYAG 2 cut(s) 598, 669
BstSLI GKGCMC 2 cut(s) 330, 395
BstUI CGCG 1 cut(s) 1141
BstX2I RGATCY 1 cut(s) 273
BstXI CCANNNNNNTGG 1 cut(s) 740
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 3 cut(s) 744, 842, 1476
BtgI CCRYGG 4 cut(s) 338, 739, 806, 1477
BtgZI GCGATG 1 cut(s) 1449
BtsCI GGATG 5 cut(s) 31, 124, 131, 217, 1168
BtsI GCAGTG 1 cut(s) 1008
BtsIMutI CAGTG 2 cut(s) 242, 1008
Cac8I GCNNGC 5 cut(s) 109, 159, 693, 844, 1302
CaiI CAGNNNCTG 1 cut(s) 1010
CciI TCATGA 1 cut(s) 1243
CfoI GCGC 1 cut(s) 1444
Csp6I GTAC 2 cut(s) 1386, 1533
CspCI CAANNNNNGTGG 3 cut(s) 538, 573, 1571
CviQI GTAC 2 cut(s) 1386, 1533
DdeI CTNAG 2 cut(s) 136, 1436
DpnI GATC 7 cut(s) 275, 452, 682, 780, 1248, 1281, 1575
DpnII GATC 7 cut(s) 273, 450, 680, 778, 1246, 1279, 1573
DraIII CACNNNGTG 1 cut(s) 1528
EaeI YGGCCR 2 cut(s) 840, 1474
Eam1104I CTCTTC 5 cut(s) 176, 316, 895, 946, 1205
EarI CTCTTC 5 cut(s) 176, 316, 895, 946, 1205
EciI GGCGGA 1 cut(s) 856
Eco130I CCWWGG 2 cut(s) 338, 739
Eco24I GRGCYC 2 cut(s) 433, 1512
Eco57I CTGAAG 3 cut(s) 237, 293, 1004
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 2 cut(s) 338, 739
EcoT38I GRGCYC 2 cut(s) 433, 1512
ErhI CCWWGG 2 cut(s) 338, 739
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 3 cut(s) 452, 620, 1446
FauI CCCGC 2 cut(s) 136, 1498
FbaI TGATCA 2 cut(s) 1246, 1573
FblI GTMKAC 2 cut(s) 117, 1323
FokI GGATG 5 cut(s) 38, 111, 118, 224, 1175
FriOI GRGCYC 2 cut(s) 433, 1512
FspBI CTAG 3 cut(s) 468, 933, 1106
GlaI GCGC 1 cut(s) 1443
GsuI CTGGAG 1 cut(s) 1527
HaeII RGCGCY 1 cut(s) 1445
HaeIII GGCC 3 cut(s) 744, 842, 1476
HapII CCGG 2 cut(s) 209, 395
HhaI GCGC 1 cut(s) 1444
Hin6I GCGC 1 cut(s) 1442
HinP1I GCGC 1 cut(s) 1442
HincII GTYRAC 1 cut(s) 1324
HindII GTYRAC 1 cut(s) 1324
HinfI GANTC 2 cut(s) 95, 261
HpaII CCGG 2 cut(s) 209, 395
HphI GGTGA 6 cut(s) 71, 382, 491, 1103, 1493, 1511
Hpy166II GTNNAC 3 cut(s) 118, 412, 1324
Hpy188I TCNGA 6 cut(s) 45, 89, 532, 1023, 1033, 1578
Hpy188III TCNNGA 5 cut(s) 454, 468, 766, 1244, 1339
Hpy8I GTNNAC 3 cut(s) 118, 412, 1324
Hpy99I CGWCG 5 cut(s) 1267, 1270, 1273, 1328, 1340
HpyAV CCTTC 6 cut(s) 317, 373, 809, 821, 1018, 1074
HpyCH4III ACNGT 5 cut(s) 115, 479, 611, 1328, 1481
HpyCH4V TGCA 3 cut(s) 279, 521, 671
HpyF10VI GCNNNNNNNGC 8 cut(s) 137, 163, 334, 343, 790, 1115, 1294, 1297
HpyF3I CTNAG 2 cut(s) 136, 1436
HspAI GCGC 1 cut(s) 1442
Ksp22I TGATCA 2 cut(s) 1246, 1573
Kzo9I GATC 7 cut(s) 273, 450, 680, 778, 1246, 1279, 1573
LguI GCTCTTC 1 cut(s) 946
LmnI GCTCC 1 cut(s) 801
LweI GCATC 7 cut(s) 93, 140, 202, 628, 793, 1037, 1472
MaeI CTAG 3 cut(s) 468, 933, 1106
MaeIII GTNAC 1 cut(s) 1142
MalI GATC 7 cut(s) 275, 452, 682, 780, 1248, 1281, 1575
MboI GATC 7 cut(s) 273, 450, 680, 778, 1246, 1279, 1573
MflI RGATCY 1 cut(s) 273
MhlI GDGCHC 4 cut(s) 330, 395, 433, 1512
MluCI AATT 4 cut(s) 144, 1314, 1330, 1366
MlyI GAGTC 1 cut(s) 104
MmeI TCCRAC 3 cut(s) 510, 994, 1152
MroXI GAANNNNTTC 2 cut(s) 260, 461
MseI TTAA 2 cut(s) 65, 1344
MslI CAYNNNNRTG 2 cut(s) 738, 1524
MspA1I CMGCKG 3 cut(s) 674, 761, 1547
MspI CCGG 2 cut(s) 209, 395
MspR9I CCNGG 3 cut(s) 195, 209, 395
MvaI CCWGG 1 cut(s) 195
MvnI CGCG 1 cut(s) 1141
MwoI GCNNNNNNNGC 8 cut(s) 137, 163, 334, 343, 790, 1115, 1294, 1297
NciI CCSGG 2 cut(s) 209, 395
NcoI CCATGG 2 cut(s) 338, 739
NdeII GATC 7 cut(s) 273, 450, 680, 778, 1246, 1279, 1573
NlaIV GGNNCC 2 cut(s) 399, 589
NmuCI GTSAC 1 cut(s) 1142
NspI RCATGY 3 cut(s) 408, 1003, 1126
PagI TCATGA 1 cut(s) 1243
PciI ACATGT 3 cut(s) 404, 999, 1122
PciSI GCTCTTC 1 cut(s) 946
PcsI WCGNNNNNNNCGW 2 cut(s) 1268, 1332
PdmI GAANNNNTTC 2 cut(s) 260, 461
PfeI GAWTC 1 cut(s) 261
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PscI ACATGT 3 cut(s) 404, 999, 1122
PshBI ATTAAT 1 cut(s) 1344
PsiI TTATAA 2 cut(s) 1371, 1554
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 2 cut(s) 399, 589
PstI CTGCAG 1 cut(s) 673
PstNI CAGNNNCTG 1 cut(s) 1010
PsuI RGATCY 1 cut(s) 273
PvuII CAGCTG 3 cut(s) 674, 761, 1547
RsaI GTAC 2 cut(s) 1387, 1534
RsaNI GTAC 2 cut(s) 1386, 1533
RseI CAYNNNNRTG 2 cut(s) 738, 1524
SalI GTCGAC 1 cut(s) 1322
SapI GCTCTTC 1 cut(s) 946
SaqAI TTAA 2 cut(s) 65, 1344
Sau3AI GATC 7 cut(s) 273, 450, 680, 778, 1246, 1279, 1573
ScaI AGTACT 2 cut(s) 1387, 1534
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 195, 209, 395
SduI GDGCHC 4 cut(s) 330, 395, 433, 1512
SfaNI GCATC 7 cut(s) 93, 140, 202, 628, 793, 1037, 1472
SfcI CTRYAG 2 cut(s) 598, 669
SmiMI CAYNNNNRTG 2 cut(s) 738, 1524
SmlI CTYRAG 1 cut(s) 79
SmoI CTYRAG 1 cut(s) 79
Sse9I AATT 4 cut(s) 144, 1314, 1330, 1366
SspI AATATT 1 cut(s) 905
SspMI CTAG 3 cut(s) 468, 933, 1106
StyD4I CCNGG 3 cut(s) 193, 207, 393
StyI CCWWGG 2 cut(s) 338, 739
TaaI ACNGT 5 cut(s) 115, 479, 611, 1328, 1481
TaqI TCGA 3 cut(s) 1323, 1338, 1512
TasI AATT 4 cut(s) 144, 1314, 1330, 1366
TatI WGTACW 2 cut(s) 1385, 1532
TauI GCSGC 3 cut(s) 834, 842, 1476
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 2 cut(s) 65, 1344
Tru9I TTAA 2 cut(s) 65, 1344
TscAI CASTG 2 cut(s) 249, 1015
TseFI GTSAC 1 cut(s) 1142
Tsp45I GTSAC 1 cut(s) 1142
TspDTI ATGAA 8 cut(s) 20, 305, 432, 604, 804, 872, 882, 1440
TspGWI ACGGA 1 cut(s) 795
TspRI CASTG 2 cut(s) 249, 1015
VspI ATTAAT 1 cut(s) 1344
XapI RAATTY 1 cut(s) 144
XbaI TCTAGA 1 cut(s) 467
XceI RCATGY 3 cut(s) 408, 1003, 1126
XmiI GTMKAC 2 cut(s) 117, 1323
XmnI GAANNNNTTC 2 cut(s) 260, 461
XspI CTAG 3 cut(s) 468, 933, 1106
ZrmI AGTACT 2 cut(s) 1387, 1534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.