RLG00000010435

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009657
Physical Location & Seq
Forward (+)
1626695 .. 1629358
2664 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010435

Sequence Viewer

Length: 1620 bp
ATGCAAACCACTAGTTACCAGCAAGGTGAAAATCAATTAATTATGATGAAAGGTTTTGCAGTGGATGAGAACATGTCCAATCTGACTTGTGCATCTGGAGATTTAAGCGCTTCTGCCAATTCGAGTATCAGAAATGATTCATCATCTTCAACACAACAGCAACAACCACCGCAAAAGAAAAAGAGAAATCTTCCAGGCAACCCAGATCCAGACGCTGAAGTGATAGCTTTGTCCCCCAAATCACTCATGGCAACGAACCGATTCGTTTGCGAGGTCTGCAACAAAGGGTTTCAGAGAGAGCAGAACCTTCAGCTTCACCGGAGAGGCCACAATCTGCCATGGAAGCTAAAGCAAAGGACAAACAAGGAGGTGAGGAAGAAGGTGTATCTGTGCCCGGAACCGACATGTGTCCACCATGACCCGTCGAGGGCTCTTGGAGACCTCACAGGGATCAAGAAGCACTTTTCCAGAAAGCACGGTGAGAAGAAGTGGAAATGTGAGAAGTGCTCGAAGCGATATGCGGTTCAATCTGATTGGAAAGCTCATTCTAAGATCTGTGGCACAAGAGAGTATCGATGTGACTGTGGAACCCTTTTCTCTAGGAGGGACAGTTTCATCACTCACAGAGCCTTCTGTGACGCTTTGGCACAAGAGAACAGCAACGCAGCTAGAGCCATAAACCCACTTCTCTCCTCATCACTGCCCCAACTCCACGGTGGCTTCCAACCTCATCACCTATCCCATCATCACGTGAAGAGAGAAGTACAAGATCTTCAGCTACAGCTTCCGCCGTGGATGGGAGGCGAAGCAGCCGCCTCAGCTGGTCCATCTCATCACCTCTCTAGCAATATAAACCTATCCTCATCGTCACCATTGTTCTCCACCTCTTCATTCGACCACCAGCAGTCTTTTCTGCACCAAAACCCTAACCCTAGCTCATCGGATTTCCAGCCCTCAGCTTCTGCTCACATGTCAGCCACTGCTTTGCTCCAAAAGGCGTCAGAAATGGGCGCCACTGTAAGCAAGCCTTCCTCGATGTTTCTCAAGCCCCATGACCAACAACACCAAGCTTCCCACATGATGCCGGAAACGACAGCGTTTGGCTCTTTCATGGAGCAGGCTGGTGCCAACTCTGGCGGCGCAGCAGCAGCTTCTAATAACTCTTTTTTGCATGATATGATGATGATGACTTCTCATCTGCAGCAATCTTCTTCCGCGGTTCGTGGCGGGTTTGACCAGCTTTCATCCTCGTCATCCTTTGGAGATCATCATCATCAGGGTTTCAATGGATCGATGGTGATGGGTAGTTTCGCTGAAAATGTTAACCCTTCCCCTCAGAAGAAACTAATCACCGGGGAATCTCAGCTTCGGAGGACTAGTAGTACTACTGATGGAGGAGGAGGTGGCGGCGGCAGCGGCGGTGGCGGTGGCGGGATGAATGAGGGAATGACAAGAGATTTCTTGGGTCTGAGAGCATTTTCTTCTCAAGATGATCAAGACTTTTTCAACATGGCTGCTGCTCATGGACTCGACCAGCATGTGGTGAATTCTACTTCTTCAGCTAATTACAATGGACAGCAGCATAATCATCAGAACCAGACTTCTTGGCAAGGTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2 zf-C2H2_jaz

Protein Analysis

540

Amino Acids

58.72

Weight (kDa)

8.79

Isoelectric Point (pI)

50.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 125 - 159 6.1e-23 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 163 - 187 3.9e-15 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 190 - 225 3.6e-23 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1010, 1124
AccB7I CCANNNNNTGG 1 cut(s) 1540
AccII CGCG 1 cut(s) 1217
AclWI GGATC 3 cut(s) 200, 458, 1297
AcsI RAATTY 1 cut(s) 1546
AcuI CTGAAG 4 cut(s) 237, 293, 758, 1542
AcvI CACGTG 1 cut(s) 751
AcyI GRCGYC 2 cut(s) 998, 1011
AfaI GTAC 2 cut(s) 765, 1384
AfeI AGCGCT 1 cut(s) 109
AfiI CCNNNNNNNGG 3 cut(s) 427, 797, 1540
AflIII ACRYGT 3 cut(s) 72, 404, 969
AgsI TTSAA 4 cut(s) 150, 527, 1285, 1507
AhlI ACTAGT 2 cut(s) 11, 1376
AjnI CCWGG 1 cut(s) 193
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 1 cut(s) 432
AlwI GGATC 3 cut(s) 200, 458, 1297
AlwNI CAGNNNCTG 3 cut(s) 215, 962, 980
Aor51HI AGCGCT 1 cut(s) 109
AoxI GGCC 1 cut(s) 325
ApoI RAATTY 1 cut(s) 1546
AseI ATTAAT 1 cut(s) 38
Asp700I GAANNNNTTC 2 cut(s) 136, 260
AspLEI GCGC 3 cut(s) 110, 1013, 1142
AspS9I GGNCC 1 cut(s) 824
AsuC2I CCSGG 2 cut(s) 395, 1354
AvaII GGWCC 1 cut(s) 824
BaeGI GKGCMC 1 cut(s) 395
BanI GGYRCC 2 cut(s) 1010, 1124
BanII GRGCYC 1 cut(s) 433
BarI GAAGNNNNNNTAC 2 cut(s) 756, 788
BbrPI CACGTG 1 cut(s) 751
Bbv12I GWGCWC 1 cut(s) 509
BbvCI CCTCAGC 2 cut(s) 817, 955
BccI CCATC 6 cut(s) 750, 790, 835, 1288, 1294, 1385
BceAI ACGGC 1 cut(s) 775
BcgI CGANNNNNNTGC 2 cut(s) 249, 283
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 1 cut(s) 1492
BcnI CCSGG 2 cut(s) 395, 1354
BcoDI GTCTC 1 cut(s) 432
BcuI ACTAGT 2 cut(s) 11, 1376
BfaI CTAG 6 cut(s) 12, 600, 669, 843, 933, 1377
BfmI CTRYAG 2 cut(s) 779, 1199
BfoI RGCGCY 2 cut(s) 111, 1014
BglII AGATCT 2 cut(s) 552, 769
BmcAI AGTACT 1 cut(s) 1384
Bme1390I CCNGG 3 cut(s) 195, 395, 1354
Bme18I GGWCC 1 cut(s) 824
BmgT120I GGNCC 1 cut(s) 824
BmiI GGNNCC 4 cut(s) 399, 589, 1012, 1126
BmrFI CCNGG 3 cut(s) 195, 395, 1354
BmsI GCATC 2 cut(s) 101, 1071
BoxI GACNNNNGTC 1 cut(s) 407
BplI GAGNNNNNCTC 2 cut(s) 491, 523
BpmI CTGGAG 1 cut(s) 117
Bpu10I CCTNAGC 2 cut(s) 817, 955
BpuEI CTTGAG 2 cut(s) 1028, 1470
BpuMI CCSGG 2 cut(s) 395, 1354
Bsa29I ATCGAT 2 cut(s) 574, 1292
BsaAI YACGTR 1 cut(s) 751
BsaBI GATNNNNATC 1 cut(s) 1269
BsaHI GRCGYC 2 cut(s) 998, 1011
BsaI GGTCTC 1 cut(s) 432
BsaJI CCNNGG 5 cut(s) 338, 712, 791, 1215, 1353
BsaWI WCCGGW 1 cut(s) 318
BsaXI ACNNNNNCTCC 4 cut(s) 595, 625, 1363, 1393
Bsc4I CCNNNNNNNGG 3 cut(s) 427, 797, 1540
Bse8I GATNNNNATC 1 cut(s) 1269
BseBI CCWGG 1 cut(s) 195
BseCI ATCGAT 2 cut(s) 574, 1292
BseDI CCNNGG 5 cut(s) 338, 712, 791, 1215, 1353
BseGI GGATG 5 cut(s) 70, 801, 1244, 1253, 1440
BseJI GATNNNNATC 1 cut(s) 1269
BseLI CCNNNNNNNGG 3 cut(s) 427, 797, 1540
BseMII CTCAG 5 cut(s) 831, 969, 1349, 1376, 1460
BseRI GAGGAG 3 cut(s) 682, 1410, 1413
BseSI GKGCMC 1 cut(s) 395
BsgI GTGCAG 1 cut(s) 899
Bsh1236I CGCG 1 cut(s) 1217
BshFI GGCC 1 cut(s) 327
BshNI GGYRCC 2 cut(s) 1010, 1124
BshVI ATCGAT 2 cut(s) 574, 1292
BsiHKAI GWGCWC 1 cut(s) 509
BsiSI CCGG 4 cut(s) 319, 395, 1085, 1353
BslFI GGGAC 2 cut(s) 217, 620
BslI CCNNNNNNNGG 3 cut(s) 427, 797, 1540
BsmAI GTCTC 1 cut(s) 432
BsmFI GGGAC 2 cut(s) 217, 620
BsnI GGCC 1 cut(s) 327
Bso31I GGTCTC 1 cut(s) 432
Bsp1286I GDGCHC 3 cut(s) 395, 433, 509
Bsp143I GATC 7 cut(s) 205, 450, 552, 769, 1264, 1289, 1492
Bsp19I CCATGG 1 cut(s) 338
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 5 cut(s) 830, 968, 1348, 1375, 1461
BspDI ATCGAT 2 cut(s) 574, 1292
BspFNI CGCG 1 cut(s) 1217
BspLI GGNNCC 4 cut(s) 399, 589, 1012, 1126
BspMAI CTGCAG 1 cut(s) 1203
BspPI GGATC 3 cut(s) 200, 458, 1297
BspT107I GGYRCC 2 cut(s) 1010, 1124
BspTNI GGTCTC 1 cut(s) 432
BssECI CCNNGG 5 cut(s) 338, 712, 791, 1215, 1353
BssMI GATC 7 cut(s) 205, 450, 552, 769, 1264, 1289, 1492
BssNI GRCGYC 2 cut(s) 998, 1011
BssT1I CCWWGG 1 cut(s) 338
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 5 cut(s) 479, 584, 611, 716, 1018
Bst6I CTCTTC 2 cut(s) 749, 892
BstACI GRCGYC 2 cut(s) 998, 1011
BstBAI YACGTR 1 cut(s) 751
BstC8I GCNNGC 2 cut(s) 1025, 1119
BstDEI CTNAG 6 cut(s) 549, 817, 955, 1335, 1362, 1469
BstDSI CCRYGG 4 cut(s) 338, 712, 791, 1215
BstF5I GGATG 5 cut(s) 70, 801, 1244, 1253, 1440
BstFNI CGCG 1 cut(s) 1217
BstH2I RGCGCY 2 cut(s) 111, 1014
BstHHI GCGC 3 cut(s) 110, 1013, 1142
BstKTI GATC 7 cut(s) 208, 453, 555, 772, 1267, 1292, 1495
BstMAI GTCTC 1 cut(s) 432
BstMBI GATC 7 cut(s) 205, 450, 552, 769, 1264, 1289, 1492
BstMWI GCNNNNNNNGC 8 cut(s) 276, 343, 671, 818, 1148, 1413, 1416, 1422
BstNI CCWGG 1 cut(s) 195
BstNSI RCATGY 4 cut(s) 76, 408, 973, 1541
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 3 cut(s) 193, 393, 1352
BstSFI CTRYAG 2 cut(s) 779, 1199
BstSLI GKGCMC 1 cut(s) 395
BstUI CGCG 1 cut(s) 1217
BstX2I RGATCY 3 cut(s) 205, 552, 769
BstYI RGATCY 3 cut(s) 205, 552, 769
Bsu15I ATCGAT 2 cut(s) 574, 1292
BsuRI GGCC 1 cut(s) 327
BsuTUI ATCGAT 2 cut(s) 574, 1292
BtgI CCRYGG 4 cut(s) 338, 712, 791, 1215
BtsCI GGATG 5 cut(s) 70, 801, 1244, 1253, 1440
BtsI GCAGTG 3 cut(s) 66, 698, 978
BtsIMutI CAGTG 4 cut(s) 66, 698, 978, 1014
Cac8I GCNNGC 2 cut(s) 1025, 1119
CaiI CAGNNNCTG 3 cut(s) 215, 962, 980
CfoI GCGC 3 cut(s) 110, 1013, 1142
Cfr13I GGNCC 1 cut(s) 824
Cfr42I CCGCGG 1 cut(s) 1218
ClaI ATCGAT 2 cut(s) 574, 1292
CseI GACGC 3 cut(s) 221, 647, 987
Csp6I GTAC 2 cut(s) 764, 1383
CviQI GTAC 2 cut(s) 764, 1383
DdeI CTNAG 6 cut(s) 549, 817, 955, 1335, 1362, 1469
DinI GGCGCC 1 cut(s) 1012
DpnI GATC 7 cut(s) 207, 452, 554, 771, 1266, 1291, 1494
DpnII GATC 7 cut(s) 205, 450, 552, 769, 1264, 1289, 1492
Eam1104I CTCTTC 2 cut(s) 749, 892
EarI CTCTTC 2 cut(s) 749, 892
EciI GGCGGA 1 cut(s) 777
Eco130I CCWWGG 1 cut(s) 338
Eco24I GRGCYC 1 cut(s) 433
Eco31I GGTCTC 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 824
Eco47III AGCGCT 1 cut(s) 109
Eco57I CTGAAG 4 cut(s) 237, 293, 758, 1542
Eco72I CACGTG 1 cut(s) 751
EcoRI GAATTC 1 cut(s) 1546
EcoRII CCWGG 1 cut(s) 193
EcoT14I CCWWGG 1 cut(s) 338
EcoT38I GRGCYC 1 cut(s) 433
EgeI GGCGCC 1 cut(s) 1012
EheI GGCGCC 1 cut(s) 1012
ErhI CCWWGG 1 cut(s) 338
FalI AAGNNNNNCTT 4 cut(s) 446, 478, 1012, 1044
FaqI GGGAC 2 cut(s) 217, 620
FauI CCCGC 2 cut(s) 1220, 1424
FbaI TGATCA 1 cut(s) 1492
FokI GGATG 5 cut(s) 77, 808, 1231, 1240, 1447
FriOI GRGCYC 1 cut(s) 433
FspBI CTAG 6 cut(s) 12, 600, 669, 843, 933, 1377
GlaI GCGC 3 cut(s) 109, 1012, 1141
GsuI CTGGAG 1 cut(s) 117
HaeII RGCGCY 2 cut(s) 111, 1014
HaeIII GGCC 1 cut(s) 327
HapII CCGG 4 cut(s) 319, 395, 1085, 1353
HgaI GACGC 3 cut(s) 221, 647, 987
HhaI GCGC 3 cut(s) 110, 1013, 1142
Hin1I GRCGYC 2 cut(s) 998, 1011
Hin6I GCGC 3 cut(s) 108, 1011, 1140
HinP1I GCGC 3 cut(s) 108, 1011, 1140
HincII GTYRAC 1 cut(s) 1324
HindII GTYRAC 1 cut(s) 1324
HindIII AAGCTT 1 cut(s) 1068
HinfI GANTC 4 cut(s) 137, 261, 1358, 1527
HpaI GTTAAC 1 cut(s) 1324
HpaII CCGG 4 cut(s) 319, 395, 1085, 1353
Hpy166II GTNNAC 2 cut(s) 412, 1324
Hpy188III TCNNGA 6 cut(s) 96, 209, 454, 468, 1487, 1496
Hpy8I GTNNAC 2 cut(s) 412, 1324
Hpy99I CGWCG 1 cut(s) 427
HpyAV CCTTC 5 cut(s) 317, 373, 640, 1038, 1338
HpyCH4III ACNGT 5 cut(s) 479, 584, 611, 716, 1018
HpyCH4IV ACGT 1 cut(s) 750
HpyCH4V TGCA 7 cut(s) 4, 59, 92, 279, 916, 1171, 1201
HpyF10VI GCNNNNNNNGC 8 cut(s) 276, 343, 671, 818, 1148, 1413, 1416, 1422
HpyF3I CTNAG 6 cut(s) 549, 817, 955, 1335, 1362, 1469
HpySE526I ACGT 1 cut(s) 750
Hsp92I GRCGYC 2 cut(s) 998, 1011
HspAI GCGC 3 cut(s) 108, 1011, 1140
KasI GGCGCC 1 cut(s) 1010
Ksp22I TGATCA 1 cut(s) 1492
KspAI GTTAAC 1 cut(s) 1324
KspI CCGCGG 1 cut(s) 1218
Kzo9I GATC 7 cut(s) 205, 450, 552, 769, 1264, 1289, 1492
LmnI GCTCC 2 cut(s) 993, 1114
LweI GCATC 2 cut(s) 101, 1071
MaeI CTAG 6 cut(s) 12, 600, 669, 843, 933, 1377
MaeII ACGT 1 cut(s) 750
MaeIII GTNAC 4 cut(s) 14, 578, 635, 867
MalI GATC 7 cut(s) 207, 452, 554, 771, 1266, 1291, 1494
MboI GATC 7 cut(s) 205, 450, 552, 769, 1264, 1289, 1492
MflI RGATCY 3 cut(s) 205, 552, 769
MhlI GDGCHC 3 cut(s) 395, 433, 509
MluCI AATT 6 cut(s) 35, 39, 118, 1546, 1564, 1615
Mly113I GGCGCC 1 cut(s) 1011
MlyI GAGTC 1 cut(s) 1521
MmeI TCCRAC 1 cut(s) 748
MroXI GAANNNNTTC 2 cut(s) 136, 260
MseI TTAA 3 cut(s) 38, 104, 1323
MspA1I CMGCKG 3 cut(s) 821, 1217, 1416
MspI CCGG 4 cut(s) 319, 395, 1085, 1353
MspR9I CCNGG 3 cut(s) 195, 395, 1354
MvaI CCWGG 1 cut(s) 195
MvnI CGCG 1 cut(s) 1217
MwoI GCNNNNNNNGC 8 cut(s) 276, 343, 671, 818, 1148, 1413, 1416, 1422
NarI GGCGCC 1 cut(s) 1011
NciI CCSGG 2 cut(s) 395, 1354
NcoI CCATGG 1 cut(s) 338
NdeII GATC 7 cut(s) 205, 450, 552, 769, 1264, 1289, 1492
NlaIV GGNNCC 4 cut(s) 399, 589, 1012, 1126
NmuCI GTSAC 3 cut(s) 578, 635, 867
NspI RCATGY 4 cut(s) 76, 408, 973, 1541
PciI ACATGT 3 cut(s) 72, 404, 969
PdmI GAANNNNTTC 2 cut(s) 136, 260
PfeI GAWTC 3 cut(s) 137, 261, 1358
PflMI CCANNNNNTGG 1 cut(s) 1540
PleI GAGTC 1 cut(s) 1521
PluTI GGCGCC 1 cut(s) 1014
PmaCI CACGTG 1 cut(s) 751
PmlI CACGTG 1 cut(s) 751
PpsI GAGTC 1 cut(s) 1521
Ppu21I YACGTR 1 cut(s) 751
PscI ACATGT 3 cut(s) 72, 404, 969
PshAI GACNNNNGTC 1 cut(s) 407
PshBI ATTAAT 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 193
PspCI CACGTG 1 cut(s) 751
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 4 cut(s) 399, 589, 1012, 1126
PspPI GGNCC 1 cut(s) 824
PstI CTGCAG 1 cut(s) 1203
PstNI CAGNNNCTG 3 cut(s) 215, 962, 980
PsuI RGATCY 3 cut(s) 205, 552, 769
PvuII CAGCTG 1 cut(s) 821
RsaI GTAC 2 cut(s) 765, 1384
RsaNI GTAC 2 cut(s) 764, 1383
SacII CCGCGG 1 cut(s) 1218
SaqAI TTAA 3 cut(s) 38, 104, 1323
Sau3AI GATC 7 cut(s) 205, 450, 552, 769, 1264, 1289, 1492
Sau96I GGNCC 1 cut(s) 824
ScaI AGTACT 1 cut(s) 1384
SchI GAGTC 1 cut(s) 1521
ScrFI CCNGG 3 cut(s) 195, 395, 1354
SduI GDGCHC 3 cut(s) 395, 433, 509
SfaNI GCATC 2 cut(s) 101, 1071
SfcI CTRYAG 2 cut(s) 779, 1199
SfoI GGCGCC 1 cut(s) 1012
Sfr303I CCGCGG 1 cut(s) 1218
SgrBI CCGCGG 1 cut(s) 1218
SinI GGWCC 1 cut(s) 824
SmlI CTYRAG 2 cut(s) 1043, 1485
SmoI CTYRAG 2 cut(s) 1043, 1485
SpeI ACTAGT 2 cut(s) 11, 1376
Sse9I AATT 6 cut(s) 35, 39, 118, 1546, 1564, 1615
SspDI GGCGCC 1 cut(s) 1010
SspMI CTAG 6 cut(s) 12, 600, 669, 843, 933, 1377
StyD4I CCNGG 3 cut(s) 193, 393, 1352
StyI CCWWGG 1 cut(s) 338
TaaI ACNGT 5 cut(s) 479, 584, 611, 716, 1018
TaiI ACGT 1 cut(s) 753
TaqI TCGA 8 cut(s) 122, 425, 509, 574, 894, 1034, 1292, 1530
TasI AATT 6 cut(s) 35, 39, 118, 1546, 1564, 1615
TatI WGTACW 2 cut(s) 763, 1382
TauI GCSGC 5 cut(s) 815, 1140, 1410, 1413, 1419
TfiI GAWTC 3 cut(s) 137, 261, 1358
Tru1I TTAA 3 cut(s) 38, 104, 1323
Tru9I TTAA 3 cut(s) 38, 104, 1323
TscAI CASTG 4 cut(s) 66, 705, 985, 1021
TseFI GTSAC 3 cut(s) 578, 635, 867
Tsp45I GTSAC 3 cut(s) 578, 635, 867
TspDTI ATGAA 7 cut(s) 62, 129, 604, 879, 1099, 1233, 1451
TspRI CASTG 4 cut(s) 66, 705, 985, 1021
Van91I CCANNNNNTGG 1 cut(s) 1540
VpaK11BI GGWCC 1 cut(s) 824
VspI ATTAAT 1 cut(s) 38
XapI RAATTY 1 cut(s) 1546
XceI RCATGY 4 cut(s) 76, 408, 973, 1541
XcmI CCANNNNNNNNNTGG 2 cut(s) 244, 713
XmnI GAANNNNTTC 2 cut(s) 136, 260
XspI CTAG 6 cut(s) 12, 600, 669, 843, 933, 1377
ZrmI AGTACT 1 cut(s) 1384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.