pycom08g05230

ZINC FINGER protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0009657
Physical Location & Seq
Reverse (-)
4028279 .. 4030625
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g05230.1

Sequence Viewer

Length: 1587 bp
ATGGTGGATGAGAATATGTCAAATTTGACTTGTGCTTCTGGTGATTTAAGTGCTTCTAACTCAAGCATCAGAAATGAGTCATCATCTGCTGGCACAGTCTACCCTCAGCATTCCTCATCCTCAACACAAATTCAGCAGCAACAAGCACCGCCACCGCCAAAGAAGAAGAGAAATCTCCCAGGCAACCCAGACCCAGATGCTGAAGTAATAGCCTTGTCTCCAAAATCACTGATGGCAACGAATAGATTCGTGTGTGAGATCTGCAACAAAGGGTTTCAAAGAGAGCAAAACCTTCAGCTTCACAGAAGAGGGCACAATCTGCCATGGAAGCTAAAGCAAAGGACGGGGAAGGAGGTGAGGAAGAAAGTGTATCTGTGCCCGGAACCGACATGCGTCCACCATGACCCGTCGAGGGCTCTTGGGGATTTGACAGGGATCAAGAAGCACTTCTCTAGAAAGCACGGTGAGAAGAAGTGGAAGTGTGAGAAATGCTCAAAGCGTTATGCGGTTCAGTCAGATTGGAAAGCGCACTCCAAAGTCTGTGGCACAAGGGAGTATCGATGTGATTGTGGAACCCTTTTCTCTAGGAGGGACAGTTTTATCACACACAGAGCCTTTTGCGATGCTTTAGCACAAGAGAACAATACAAGCACTGCAGCTGCCAGATCAGCAATGCCAGCCTCAATCAACCCTCTTCTCTCCTCACTCCCCCAAATCCATAGCCATGGCCTCCAAGCTCTATCAGCTGTCAAGAGAGAACAAGATCAGCATCAGCACCAGCAGCACCAGCACCCGCAGCACCTTCCACCGTGGCTTTCTTGCTTGCCGGAAGGTGGCGAAGCCGCCAGCCTCCCTACTATGAACCTTTCTGCCTCGCCATTATTCTCCACCTCTTCATTCCAGCAATATTATTCTTTTGGCCAAAACCCTAACCCTAGCAGCTCTTCTACTACCCTACTCCCCGATAACTTCCAAGTTCAACAAACCCCCGCTCCCCCTCACATGTCAGCCACCGCCTTGCTTCAGAAGGCATCAGAAATGGGAGCCACCATCAGCAAACCCTCCCCTTCCCCATATTTTCTCAAACCACCCACCCACCAATCCCACCAAGCTCACATGTCCAACGAAACTTCGCGTGACCAAGCAATTCTATTGGATGATGGGTTTGGAAACAATGAGAACAAATCTTCACTCCTGCATGATATGATGATGATGAGTGGCTCTCAGGGTTTTCATCATGATCGTCATCAAGTCTCATCGTCATCGTTCGGAGATCACCCTCAGCAGCAGCAGGCTTTCAATGGAATTATGGTCGACGGTAATTTCGTCGAGATTAATAATAATAATCCTCCACAGAATTATAATAAACCAAGAAGTACTGACAACAACAACGAGGGTTTGACAAGAGATTTCCTGGGACTTAGAGCGCCATTTTCATCAGCAGCATCACATGGCGGCCATGGAGATTTTTTCAGTATTAATATGGCAGGGCTGGATCATCACCATGTGAGTACTTCTTCTTCTCCAGCTACTTATAACAACAATTGCCCGCAGAATGATCAGAACCAAACATCGTGGCAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C2H2 zf-C2H2_jaz

Protein Analysis

529

Amino Acids

58.39

Weight (kDa)

8.61

Isoelectric Point (pI)

52.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2H2-2nd_BIRD-IDD PF22996 120 - 154 5.9e-23 BIRD-IDD transcription factor, second C2H2 zinc finger
C2CH-3rd_BIRD-IDD PF22995 158 - 182 1.5e-14 BIRD-IDD transcription factor, third C2HC zinc finger
C2CH-4th_BIRD-IDD PF22992 185 - 221 1e-22 BIRD-IDD transcription factor, fourth C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1362, 1536
AccBSI CCGCTC 1 cut(s) 992
AccI GTMKAC 2 cut(s) 99, 1314
AccII CGCG 1 cut(s) 1135
AciI CCGC 9 cut(s) 149, 155, 506, 794, 843, 990, 1014, 1455, 1550
AclWI GGATC 2 cut(s) 443, 1503
AcoI YGGCCR 2 cut(s) 919, 1456
AcsI RAATTY 2 cut(s) 22, 129
AcuI CTGAAG 3 cut(s) 222, 278, 1007
AdeI CACNNNGTG 1 cut(s) 1507
AfaI GTAC 2 cut(s) 1378, 1513
AfiI CCNNNNNNNGG 2 cut(s) 412, 833
AflIII ACRYGT 2 cut(s) 1002, 1116
AgsI TTSAA 3 cut(s) 278, 980, 1300
AjnI CCWGG 2 cut(s) 178, 1413
AluBI AGCT 8 cut(s) 298, 331, 659, 737, 746, 942, 1112, 1529
AluI AGCT 8 cut(s) 298, 331, 659, 737, 746, 942, 1112, 1529
Alw26I GTCTC 2 cut(s) 222, 1258
AlwI GGATC 2 cut(s) 443, 1503
AlwNI CAGNNNCTG 1 cut(s) 200
AoxI GGCC 3 cut(s) 727, 919, 1456
ApeKI GCWGC 9 cut(s) 136, 656, 659, 781, 796, 939, 1285, 1288, 1442
ApoI RAATTY 2 cut(s) 22, 129
ArsI GACNNNNNNTTYG 2 cut(s) 1307, 1339
AseI ATTAAT 2 cut(s) 1335, 1479
Asp700I GAANNNNTTC 2 cut(s) 245, 446
AspLEI GCGC 2 cut(s) 529, 1429
AsuC2I CCSGG 1 cut(s) 380
AsuHPI GGTGA 5 cut(s) 53, 367, 476, 1268, 1493
BaeGI GKGCMC 2 cut(s) 315, 380
BalI TGGCCA 1 cut(s) 921
BanII GRGCYC 1 cut(s) 418
BarI GAAGNNNNNNTAC 2 cut(s) 1504, 1536
BbvCI CCTCAGC 2 cut(s) 105, 1281
BbvI GCAGC 9 cut(s) 148, 646, 668, 793, 808, 951, 1297, 1300, 1454
BccI CCATC 3 cut(s) 226, 1058, 1154
BciT130I CCWGG 2 cut(s) 180, 1415
BclI TGATCA 1 cut(s) 1558
BcnI CCSGG 1 cut(s) 380
BcoDI GTCTC 2 cut(s) 222, 1258
BfaI CTAG 3 cut(s) 453, 585, 936
BfmI CTRYAG 1 cut(s) 654
BfoI RGCGCY 1 cut(s) 1430
BglII AGATCT 1 cut(s) 258
BmcAI AGTACT 2 cut(s) 1378, 1513
Bme1390I CCNGG 3 cut(s) 180, 380, 1415
BmiI GGNNCC 3 cut(s) 384, 574, 1045
BmrFI CCNGG 3 cut(s) 180, 380, 1415
BmsI GCATC 6 cut(s) 75, 187, 613, 778, 1040, 1454
BoxI GACNNNNGTC 1 cut(s) 392
BplI GAGNNNNNCTC 4 cut(s) 476, 508, 1207, 1239
BpmI CTGGAG 1 cut(s) 1509
Bpu10I CCTNAGC 2 cut(s) 105, 1281
BpuEI CTTGAG 1 cut(s) 46
BpuMI CCSGG 1 cut(s) 380
Bsa29I ATCGAT 1 cut(s) 559
BsaBI GATNNNNATC 2 cut(s) 768, 1245
BsaJI CCNNGG 6 cut(s) 178, 323, 724, 809, 1414, 1459
BsaXI ACNNNNNCTCC 4 cut(s) 580, 610, 976, 1006
Bsc4I CCNNNNNNNGG 2 cut(s) 412, 833
Bse3DI GCAATG 1 cut(s) 678
Bse8I GATNNNNATC 2 cut(s) 768, 1245
BseBI CCWGG 2 cut(s) 180, 1415
BseCI ATCGAT 1 cut(s) 559
BseDI CCNNGG 6 cut(s) 178, 323, 724, 809, 1414, 1459
BseGI GGATG 3 cut(s) 13, 116, 1162
BseJI GATNNNNATC 2 cut(s) 768, 1245
BseLI CCNNNNNNNGG 2 cut(s) 412, 833
BseMI GCAATG 1 cut(s) 678
BseMII CTCAG 3 cut(s) 119, 1238, 1295
BseRI GAGGAG 1 cut(s) 691
BseSI GKGCMC 2 cut(s) 315, 380
BseXI GCAGC 9 cut(s) 148, 646, 668, 793, 808, 951, 1297, 1300, 1454
Bsh1236I CGCG 1 cut(s) 1135
BshFI GGCC 3 cut(s) 729, 921, 1458
BshVI ATCGAT 1 cut(s) 559
BsiSI CCGG 2 cut(s) 380, 827
BslFI GGGAC 2 cut(s) 605, 1431
BslI CCNNNNNNNGG 2 cut(s) 412, 833
BsmAI GTCTC 2 cut(s) 222, 1258
BsmFI GGGAC 2 cut(s) 605, 1431
BsmI GAATGC 1 cut(s) 109
BsnI GGCC 3 cut(s) 729, 921, 1458
Bsp1286I GDGCHC 3 cut(s) 315, 380, 418
Bsp143I GATC 8 cut(s) 258, 435, 665, 763, 1240, 1273, 1495, 1558
Bsp19I CCATGG 3 cut(s) 323, 724, 1459
BspACI CCGC 9 cut(s) 149, 155, 506, 794, 843, 990, 1014, 1455, 1550
BspANI GGCC 3 cut(s) 729, 921, 1458
BspCNI CTCAG 3 cut(s) 118, 1237, 1294
BspDI ATCGAT 1 cut(s) 559
BspFNI CGCG 1 cut(s) 1135
BspHI TCATGA 1 cut(s) 1237
BspLI GGNNCC 3 cut(s) 384, 574, 1045
BspMAI CTGCAG 1 cut(s) 658
BspPI GGATC 2 cut(s) 443, 1503
BspQI GCTCTTC 1 cut(s) 949
BsrBI CCGCTC 1 cut(s) 992
BsrDI GCAATG 1 cut(s) 678
BssECI CCNNGG 6 cut(s) 178, 323, 724, 809, 1414, 1459
BssMI GATC 8 cut(s) 258, 435, 665, 763, 1240, 1273, 1495, 1558
BssT1I CCWWGG 3 cut(s) 323, 724, 1459
Bst2UI CCWGG 2 cut(s) 180, 1415
Bst4CI ACNGT 5 cut(s) 97, 464, 596, 810, 1319
Bst6I CTCTTC 5 cut(s) 161, 301, 699, 898, 949
BstAPI GCANNNNNTGC 1 cut(s) 319
BstC8I GCNNGC 6 cut(s) 91, 678, 824, 847, 1293, 1550
BstDEI CTNAG 4 cut(s) 105, 1224, 1281, 1421
BstDSI CCRYGG 4 cut(s) 323, 724, 809, 1459
BstF5I GGATG 3 cut(s) 13, 116, 1162
BstFNI CGCG 1 cut(s) 1135
BstH2I RGCGCY 1 cut(s) 1430
BstHHI GCGC 2 cut(s) 529, 1429
BstKTI GATC 8 cut(s) 261, 438, 668, 766, 1243, 1276, 1498, 1561
BstMAI GTCTC 2 cut(s) 222, 1258
BstMBI GATC 8 cut(s) 258, 435, 665, 763, 1240, 1273, 1495, 1558
BstMWI GCNNNNNNNGC 8 cut(s) 319, 328, 668, 677, 743, 781, 787, 796
BstNI CCWGG 2 cut(s) 180, 1415
BstNSI RCATGY 3 cut(s) 393, 1006, 1120
BstPAI GACNNNNGTC 1 cut(s) 392
BstSCI CCNGG 3 cut(s) 178, 378, 1413
BstSFI CTRYAG 1 cut(s) 654
BstSLI GKGCMC 2 cut(s) 315, 380
BstUI CGCG 1 cut(s) 1135
BstV1I GCAGC 9 cut(s) 148, 646, 668, 793, 808, 951, 1297, 1300, 1454
BstX2I RGATCY 1 cut(s) 258
BstXI CCANNNNNNTGG 1 cut(s) 725
BstYI RGATCY 1 cut(s) 258
Bsu15I ATCGAT 1 cut(s) 559
BsuRI GGCC 3 cut(s) 729, 921, 1458
BsuTUI ATCGAT 1 cut(s) 559
BtgI CCRYGG 4 cut(s) 323, 724, 809, 1459
BtgZI GCGATG 1 cut(s) 636
BtsCI GGATG 3 cut(s) 13, 116, 1162
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 2 cut(s) 227, 651
Cac8I GCNNGC 6 cut(s) 91, 678, 824, 847, 1293, 1550
CaiI CAGNNNCTG 1 cut(s) 200
CciI TCATGA 1 cut(s) 1237
CfoI GCGC 2 cut(s) 529, 1429
ClaI ATCGAT 1 cut(s) 559
CseI GACGC 1 cut(s) 382
Csp6I GTAC 2 cut(s) 1377, 1512
CspCI CAANNNNNGTGG 3 cut(s) 523, 558, 1556
CviQI GTAC 2 cut(s) 1377, 1512
DdeI CTNAG 4 cut(s) 105, 1224, 1281, 1421
DpnI GATC 8 cut(s) 260, 437, 667, 765, 1242, 1275, 1497, 1560
DpnII GATC 8 cut(s) 258, 435, 665, 763, 1240, 1273, 1495, 1558
DraIII CACNNNGTG 1 cut(s) 1507
EaeI YGGCCR 2 cut(s) 919, 1456
Eam1104I CTCTTC 5 cut(s) 161, 301, 699, 898, 949
EarI CTCTTC 5 cut(s) 161, 301, 699, 898, 949
Eco130I CCWWGG 3 cut(s) 323, 724, 1459
Eco24I GRGCYC 1 cut(s) 418
Eco57I CTGAAG 3 cut(s) 222, 278, 1007
EcoRII CCWGG 2 cut(s) 178, 1413
EcoT14I CCWWGG 3 cut(s) 323, 724, 1459
EcoT38I GRGCYC 1 cut(s) 418
ErhI CCWWGG 3 cut(s) 323, 724, 1459
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 2 cut(s) 605, 1431
FauI CCCGC 3 cut(s) 801, 997, 1557
FbaI TGATCA 1 cut(s) 1558
FblI GTMKAC 2 cut(s) 99, 1314
FokI GGATG 3 cut(s) 20, 103, 1169
FriOI GRGCYC 1 cut(s) 418
FspBI CTAG 3 cut(s) 453, 585, 936
GlaI GCGC 2 cut(s) 528, 1428
GsuI CTGGAG 1 cut(s) 1509
HaeII RGCGCY 1 cut(s) 1430
HaeIII GGCC 3 cut(s) 729, 921, 1458
HapII CCGG 2 cut(s) 380, 827
HgaI GACGC 1 cut(s) 382
HhaI GCGC 2 cut(s) 529, 1429
Hin6I GCGC 2 cut(s) 527, 1427
HinP1I GCGC 2 cut(s) 527, 1427
HincII GTYRAC 1 cut(s) 1315
HindII GTYRAC 1 cut(s) 1315
HinfI GANTC 2 cut(s) 77, 246
HpaII CCGG 2 cut(s) 380, 827
HphI GGTGA 5 cut(s) 53, 367, 476, 1268, 1493
Hpy166II GTNNAC 3 cut(s) 100, 397, 1315
Hpy188I TCNGA 6 cut(s) 71, 517, 1026, 1036, 1271, 1563
Hpy188III TCNNGA 5 cut(s) 439, 453, 751, 1238, 1330
Hpy8I GTNNAC 3 cut(s) 100, 397, 1315
Hpy99I CGWCG 3 cut(s) 412, 1319, 1331
HpyAV CCTTC 6 cut(s) 302, 343, 812, 824, 1021, 1077
HpyCH4III ACNGT 5 cut(s) 97, 464, 596, 810, 1319
HpyCH4V TGCA 3 cut(s) 264, 656, 1198
HpyF10VI GCNNNNNNNGC 8 cut(s) 319, 328, 668, 677, 743, 781, 787, 796
HpyF3I CTNAG 4 cut(s) 105, 1224, 1281, 1421
HspAI GCGC 2 cut(s) 527, 1427
Ksp22I TGATCA 1 cut(s) 1558
Kzo9I GATC 8 cut(s) 258, 435, 665, 763, 1240, 1273, 1495, 1558
LguI GCTCTTC 1 cut(s) 949
LmnI GCTCC 2 cut(s) 997, 1043
Lsp1109I GCAGC 9 cut(s) 148, 646, 668, 793, 808, 951, 1297, 1300, 1454
LweI GCATC 6 cut(s) 75, 187, 613, 778, 1040, 1454
MaeI CTAG 3 cut(s) 453, 585, 936
MaeIII GTNAC 1 cut(s) 1136
MalI GATC 8 cut(s) 260, 437, 667, 765, 1242, 1275, 1497, 1560
MbiI CCGCTC 1 cut(s) 992
MboI GATC 8 cut(s) 258, 435, 665, 763, 1240, 1273, 1495, 1558
MfeI CAATTG 1 cut(s) 1543
MflI RGATCY 1 cut(s) 258
MhlI GDGCHC 3 cut(s) 315, 380, 418
MlsI TGGCCA 1 cut(s) 921
MluCI AATT 7 cut(s) 22, 129, 1146, 1305, 1321, 1357, 1543
MluNI TGGCCA 1 cut(s) 921
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 1146
Mox20I TGGCCA 1 cut(s) 921
MroXI GAANNNNTTC 2 cut(s) 245, 446
MscI TGGCCA 1 cut(s) 921
MseI TTAA 3 cut(s) 47, 1335, 1479
MslI CAYNNNNRTG 2 cut(s) 723, 1503
Msp20I TGGCCA 1 cut(s) 921
MspA1I CMGCKG 2 cut(s) 659, 746
MspI CCGG 2 cut(s) 380, 827
MspR9I CCNGG 3 cut(s) 180, 380, 1415
MunI CAATTG 1 cut(s) 1543
Mva1269I GAATGC 1 cut(s) 109
MvaI CCWGG 2 cut(s) 180, 1415
MvnI CGCG 1 cut(s) 1135
MwoI GCNNNNNNNGC 8 cut(s) 319, 328, 668, 677, 743, 781, 787, 796
NciI CCSGG 1 cut(s) 380
NcoI CCATGG 3 cut(s) 323, 724, 1459
NdeII GATC 8 cut(s) 258, 435, 665, 763, 1240, 1273, 1495, 1558
NlaIV GGNNCC 3 cut(s) 384, 574, 1045
NmuCI GTSAC 1 cut(s) 1136
NspI RCATGY 3 cut(s) 393, 1006, 1120
PagI TCATGA 1 cut(s) 1237
PciI ACATGT 2 cut(s) 1002, 1116
PciSI GCTCTTC 1 cut(s) 949
PcsI WCGNNNNNNNCGW 1 cut(s) 1323
PctI GAATGC 1 cut(s) 109
PdmI GAANNNNTTC 2 cut(s) 245, 446
PfeI GAWTC 1 cut(s) 246
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PscI ACATGT 2 cut(s) 1002, 1116
PshAI GACNNNNGTC 1 cut(s) 392
PshBI ATTAAT 2 cut(s) 1335, 1479
PsiI TTATAA 2 cut(s) 1362, 1536
Psp6I CCWGG 2 cut(s) 178, 1413
PspGI CCWGG 2 cut(s) 178, 1413
PspN4I GGNNCC 3 cut(s) 384, 574, 1045
PstI CTGCAG 1 cut(s) 658
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 1 cut(s) 258
PvuII CAGCTG 2 cut(s) 659, 746
RsaI GTAC 2 cut(s) 1378, 1513
RsaNI GTAC 2 cut(s) 1377, 1512
RseI CAYNNNNRTG 2 cut(s) 723, 1503
SalI GTCGAC 1 cut(s) 1313
SapI GCTCTTC 1 cut(s) 949
SaqAI TTAA 3 cut(s) 47, 1335, 1479
Sau3AI GATC 8 cut(s) 258, 435, 665, 763, 1240, 1273, 1495, 1558
ScaI AGTACT 2 cut(s) 1378, 1513
SchI GAGTC 1 cut(s) 86
ScrFI CCNGG 3 cut(s) 180, 380, 1415
SduI GDGCHC 3 cut(s) 315, 380, 418
SfaNI GCATC 6 cut(s) 75, 187, 613, 778, 1040, 1454
SfcI CTRYAG 1 cut(s) 654
SmiMI CAYNNNNRTG 2 cut(s) 723, 1503
SmlI CTYRAG 1 cut(s) 61
SmoI CTYRAG 1 cut(s) 61
Sse9I AATT 7 cut(s) 22, 129, 1146, 1305, 1321, 1357, 1543
SsiI CCGC 9 cut(s) 149, 155, 506, 794, 843, 990, 1014, 1455, 1550
SspI AATATT 1 cut(s) 908
SspMI CTAG 3 cut(s) 453, 585, 936
StyD4I CCNGG 3 cut(s) 178, 378, 1413
StyI CCWWGG 3 cut(s) 323, 724, 1459
TaaI ACNGT 5 cut(s) 97, 464, 596, 810, 1319
TaqI TCGA 4 cut(s) 410, 559, 1314, 1329
TasI AATT 7 cut(s) 22, 129, 1146, 1305, 1321, 1357, 1543
TatI WGTACW 2 cut(s) 1376, 1511
TauI GCSGC 2 cut(s) 845, 1458
TfiI GAWTC 1 cut(s) 246
Tru1I TTAA 3 cut(s) 47, 1335, 1479
Tru9I TTAA 3 cut(s) 47, 1335, 1479
TscAI CASTG 2 cut(s) 234, 658
TseFI GTSAC 1 cut(s) 1136
TseI GCWGC 9 cut(s) 136, 656, 659, 781, 796, 939, 1285, 1288, 1442
Tsp45I GTSAC 1 cut(s) 1136
TspDTI ATGAA 4 cut(s) 875, 885, 1223, 1425
TspRI CASTG 2 cut(s) 234, 658
VspI ATTAAT 2 cut(s) 1335, 1479
XapI RAATTY 2 cut(s) 22, 129
XbaI TCTAGA 1 cut(s) 452
XceI RCATGY 3 cut(s) 393, 1006, 1120
XmiI GTMKAC 2 cut(s) 99, 1314
XmnI GAANNNNTTC 2 cut(s) 245, 446
XspI CTAG 3 cut(s) 453, 585, 936
ZrmI AGTACT 2 cut(s) 1378, 1513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.