MD15G1083100.v1.1

substituted 4 bases at 4 genomic stop codons

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017595
Physical Location & Seq
Forward (+)
5687501 .. 5688589
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1083100.v1.1.491

Sequence Viewer

Length: 624 bp
ATGTGCGACTTTTGGGAAAGTCAACTCCACGAGATAACTAAAGGCACCATGAACTTCATCATTTCCTTCTGCTTTTGCCTCTGCATTTCTCTCTTGCCCTCCTTTGGAACCCCCGCTGGGTTCAATTGGCATTCAAGTAAAAAAGGAGGGTGGTACAACTCACTCAAGACCTCCATTCTTGAACTTGCTGACTCTGGCATCACCCATGTCTGGCTTCCTCCTCCTCACGCTAATGGAACCGAGGATAATAATTCGTTACTATTACTCAAACATTTCAGGATTCTACCCGGAAGGCTATACTATCTCGATGCATCGAGCTATGGAAATAAGGATGAATTGAAGTCCCTATTTACTGCCATGCATGAGAAGGGAATCAAAGCCATAGCAGACATTGTGATAAACCACAGAGCAGCAGCTGTCAAACAAGATGAGAACGGACTATGGAGAATATTCGAAGGTGGAAAGCCGGATGGACGTCTTGACTTCGATGCCTCCTTGATTTGCCGGGATGACAATGACCACCCTTTTGGCACTGGCAACCCGGACACAGTAGACAACTTCCCGTTCACAGCTGACATGGACCACACAAACCCTAGCTTCAAAGCGAGTTGTCTGATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase PPR PPR_2

Protein Analysis

208

Amino Acids

23.07

Weight (kDa)

5.65

Isoelectric Point (pI)

27.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Alpha-amylase PF00128 66 - 140 8.4e-07 Alpha amylase, catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06030
malus_domestica MD00G1215700.v1.1 MD15G1083100.v1.1
prunus_persica Prupe.8G125300_v2.0.a1
pyrus_communis pycom05g07400 pycom05g07450
rosa_chinensis RchiOBHm_Chr6g0257211 RchiOBHm_Chr6g0257321
rosa_rugosa Rorug05G0574100
rosa_samantha Rh6AG092000 Rh6CG079700 Rh6DG075200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 478
AccB1I GGYRCC 1 cut(s) 44
AccI GTMKAC 1 cut(s) 552
AciI CCGC 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 475
AfaI GTAC 1 cut(s) 155
AfiI CCNNNNNNNGG 3 cut(s) 104, 117, 210
AgsI TTSAA 5 cut(s) 124, 135, 182, 340, 601
AluBI AGCT 4 cut(s) 318, 416, 572, 597
AluI AGCT 4 cut(s) 318, 416, 572, 597
AlwNI CAGNNNCTG 1 cut(s) 416
ApeKI GCWGC 2 cut(s) 410, 413
ArsI GACNNNNNNTTYG 2 cut(s) 509, 541
AspS9I GGNCC 1 cut(s) 580
AsuC2I CCSGG 3 cut(s) 288, 506, 542
AsuHPI GGTGA 1 cut(s) 193
AsuII TTCGAA 1 cut(s) 453
AvaII GGWCC 1 cut(s) 580
BanI GGYRCC 1 cut(s) 44
BauI CACGAG 1 cut(s) 29
BbvI GCAGC 2 cut(s) 422, 425
BccI CCATC 1 cut(s) 464
BcnI CCSGG 3 cut(s) 288, 506, 542
BfaI CTAG 1 cut(s) 594
BisI GCNGC 2 cut(s) 411, 414
BlsI GCNGC 2 cut(s) 412, 415
Bme1390I CCNGG 3 cut(s) 288, 506, 542
Bme18I GGWCC 1 cut(s) 580
BmgT120I GGNCC 1 cut(s) 580
BmiI GGNNCC 3 cut(s) 46, 109, 238
BmrFI CCNGG 3 cut(s) 288, 506, 542
BmsI GCATC 4 cut(s) 207, 298, 320, 478
Bpu14I TTCGAA 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 3 cut(s) 288, 506, 542
BsaHI GRCGYC 1 cut(s) 475
BsaJI CCNNGG 1 cut(s) 240
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 117, 210
Bse1I ACTGG 1 cut(s) 538
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 3 cut(s) 337, 475, 514
BseLI CCNNNNNNNGG 3 cut(s) 104, 117, 210
BseNI ACTGG 1 cut(s) 538
BseRI GAGGAG 2 cut(s) 210, 213
BseXI GCAGC 2 cut(s) 422, 425
BseYI CCCAGC 1 cut(s) 116
BshNI GGYRCC 1 cut(s) 44
BsiSI CCGG 4 cut(s) 288, 467, 505, 542
BslFI GGGAC 1 cut(s) 328
BslI CCNNNNNNNGG 3 cut(s) 104, 117, 210
BsmFI GGGAC 1 cut(s) 328
BsmI GAATGC 1 cut(s) 130
Bsp119I TTCGAA 1 cut(s) 453
BspACI CCGC 1 cut(s) 114
BspLI GGNNCC 3 cut(s) 46, 109, 238
BspT104I TTCGAA 1 cut(s) 453
BspT107I GGYRCC 1 cut(s) 44
BsrI ACTGG 1 cut(s) 538
BssECI CCNNGG 1 cut(s) 240
BssNI GRCGYC 1 cut(s) 475
BssSI CACGAG 1 cut(s) 29
Bst2BI CACGAG 1 cut(s) 29
Bst4CI ACNGT 1 cut(s) 550
BstACI GRCGYC 1 cut(s) 475
BstBI TTCGAA 1 cut(s) 453
BstF5I GGATG 3 cut(s) 337, 475, 514
BstSCI CCNGG 3 cut(s) 286, 504, 540
BstV1I GCAGC 2 cut(s) 422, 425
BstXI CCANNNNNNTGG 1 cut(s) 527
BtsCI GGATG 3 cut(s) 337, 475, 514
BtsIMutI CAGTG 1 cut(s) 531
CaiI CAGNNNCTG 1 cut(s) 416
Cfr13I GGNCC 1 cut(s) 580
Csp6I GTAC 1 cut(s) 154
CviAII CATG 5 cut(s) 49, 206, 358, 362, 577
CviJI RGCY 8 cut(s) 214, 295, 318, 380, 416, 466, 572, 597
CviKI_1 RGCY 8 cut(s) 214, 295, 318, 380, 416, 466, 572, 597
CviQI GTAC 1 cut(s) 154
Eco47I GGWCC 1 cut(s) 580
EcoT22I ATGCAT 2 cut(s) 313, 363
FaeI CATG 5 cut(s) 52, 209, 361, 365, 580
FaiI YATR 9 cut(s) 50, 207, 298, 321, 359, 363, 383, 442, 578
FaqI GGGAC 1 cut(s) 328
FatI CATG 5 cut(s) 48, 205, 357, 361, 576
FauI CCCGC 1 cut(s) 121
FblI GTMKAC 1 cut(s) 552
Fnu4HI GCNGC 2 cut(s) 411, 414
FokI GGATG 3 cut(s) 344, 482, 521
Fsp4HI GCNGC 2 cut(s) 411, 414
FspBI CTAG 1 cut(s) 594
GluI GCNGC 2 cut(s) 411, 414
GsaI CCCAGC 1 cut(s) 120
HapII CCGG 4 cut(s) 288, 467, 505, 542
Hin1I GRCGYC 1 cut(s) 475
Hin1II CATG 5 cut(s) 52, 209, 361, 365, 580
HincII GTYRAC 1 cut(s) 23
HindII GTYRAC 1 cut(s) 23
HinfI GANTC 3 cut(s) 191, 280, 372
HpaII CCGG 4 cut(s) 288, 467, 505, 542
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 3 cut(s) 23, 553, 567
Hpy188I TCNGA 1 cut(s) 615
Hpy188III TCNNGA 5 cut(s) 166, 179, 277, 305, 479
Hpy8I GTNNAC 3 cut(s) 23, 553, 567
HpyAV CCTTC 4 cut(s) 76, 285, 361, 449
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4IV ACGT 1 cut(s) 475
HpyCH4V TGCA 3 cut(s) 84, 311, 361
HpySE526I ACGT 1 cut(s) 475
Hsp92I GRCGYC 1 cut(s) 475
Hsp92II CATG 5 cut(s) 52, 209, 361, 365, 580
LpnPI CCDG 9 cut(s) 102, 180, 196, 262, 301, 480, 518, 519, 555
Lsp1109I GCAGC 2 cut(s) 422, 425
LweI GCATC 4 cut(s) 207, 298, 320, 478
MaeI CTAG 1 cut(s) 594
MaeII ACGT 1 cut(s) 475
MaeIII GTNAC 1 cut(s) 255
MfeI CAATTG 1 cut(s) 124
MluCI AATT 3 cut(s) 124, 250, 335
MlyI GAGTC 1 cut(s) 185
MnlI CCTC 9 cut(s) 89, 109, 140, 181, 228, 231, 234, 235, 502
Mph1103I ATGCAT 2 cut(s) 313, 363
MslI CAYNNNNRTG 1 cut(s) 231
MspA1I CMGCKG 3 cut(s) 116, 416, 572
MspI CCGG 4 cut(s) 288, 467, 505, 542
MspR9I CCNGG 3 cut(s) 288, 506, 542
MunI CAATTG 1 cut(s) 124
Mva1269I GAATGC 1 cut(s) 130
NciI CCSGG 3 cut(s) 288, 506, 542
NlaIII CATG 5 cut(s) 52, 209, 361, 365, 580
NlaIV GGNNCC 3 cut(s) 46, 109, 238
NsiI ATGCAT 2 cut(s) 313, 363
NspV TTCGAA 1 cut(s) 453
PctI GAATGC 1 cut(s) 130
PfeI GAWTC 2 cut(s) 280, 372
PkrI GCNGC 2 cut(s) 412, 415
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
PspFI CCCAGC 1 cut(s) 116
PspN4I GGNNCC 3 cut(s) 46, 109, 238
PspPI GGNCC 1 cut(s) 580
PstNI CAGNNNCTG 1 cut(s) 416
PvuII CAGCTG 2 cut(s) 416, 572
RsaI GTAC 1 cut(s) 155
RsaNI GTAC 1 cut(s) 154
RseI CAYNNNNRTG 1 cut(s) 231
SatI GCNGC 2 cut(s) 411, 414
Sau96I GGNCC 1 cut(s) 580
SchI GAGTC 1 cut(s) 185
ScrFI CCNGG 3 cut(s) 288, 506, 542
SetI ASST 7 cut(s) 173, 320, 418, 460, 478, 574, 599
SfaNI GCATC 4 cut(s) 207, 298, 320, 478
SfuI TTCGAA 1 cut(s) 453
SinI GGWCC 1 cut(s) 580
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
Sse9I AATT 3 cut(s) 124, 250, 335
SsiI CCGC 1 cut(s) 114
SspI AATATT 1 cut(s) 450
SspMI CTAG 1 cut(s) 594
StyD4I CCNGG 3 cut(s) 286, 504, 540
TaaI ACNGT 1 cut(s) 550
TaiI ACGT 1 cut(s) 478
TaqI TCGA 4 cut(s) 306, 314, 453, 486
TasI AATT 3 cut(s) 124, 250, 335
TfiI GAWTC 2 cut(s) 280, 372
TscAI CASTG 1 cut(s) 538
TseI GCWGC 2 cut(s) 410, 413
TspDTI ATGAA 3 cut(s) 46, 65, 348
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 1 cut(s) 538
VpaK11BI GGWCC 1 cut(s) 580
XmiI GTMKAC 1 cut(s) 552
XspI CTAG 1 cut(s) 594
ZraI GACGTC 1 cut(s) 476
Zsp2I ATGCAT 2 cut(s) 313, 363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.