RchiOBHm_Chr6g0257211

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0017595
Physical Location & Seq
Reverse (-)
12481693 .. 12484325
2633 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23070

Sequence Viewer

Length: 714 bp
ATGTTTGGCTTCCACCACCTTCTCATGCTTAGCATAGCAGATATTGTTATAGACCACAGATGTGCAGAGAAGAAAGATGAGAGACGAATATTGTGCATTTTTGAAGGAGGAACACCCGATGATCGCCTTGATTGGGGCCCGTCTCTTGTTTGCAGCGACGACACAGAGTTTTCTGATGGCAAGGGGAACCCTGATACTGGAAATAGCTGGGGTGGGGTACCTGACATTGATCACAAAAACACAAGGGTCCAGAATGAGCTCTCGGATTGGATGAATTGGTTAAAGACCGAAATTGGGTTCAGCGGATGGCGATTTGATTTTGTTCTGGGATATGCTCCAGAGTTCACCAAGTTGTATGTGGCAAACACAGAGCCTAATTTCTCAGTAGGAGAGTTTTGGAATCCCCTTGCTAAGGGAAGTGATGTGAAACCCGAGTTGAAGAACTCGAAGGGAGGGCCTCCTGGATTGATTGGAATATCGCCATGCAATAGTGTGACTTTCATAGACAATCATGATACTGGTTCCACACAAAGTGAGTGTCCATTTCCAGCAGATAAAAGTCATTCAAGGATATGCTTACATTCTTACTCATCCAGGGATCCCATCAATTGTAAGTCTTTTTTGTTGAAGAATGTTGACTTTAGTGTGTCTTGCCAAACTAGGATTAATTTTATAGTTGTAATAGGAGAGATTTCCAACTCCGAGTTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

237

Amino Acids

26.44

Weight (kDa)

4.98

Isoelectric Point (pI)

26.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06030
malus_domestica MD00G1215700.v1.1 MD15G1083100.v1.1
prunus_persica Prupe.8G125300_v2.0.a1
pyrus_communis pycom05g07400 pycom05g07450
rosa_chinensis RchiOBHm_Chr6g0257211 RchiOBHm_Chr6g0257321
rosa_rugosa Rorug05G0574100
rosa_samantha Rh6AG092000 Rh6CG079700 Rh6DG075200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 217
AccB1I GGYRCC 1 cut(s) 217
AciI CCGC 1 cut(s) 303
AclWI GGATC 2 cut(s) 593, 606
AdeI CACNNNGTG 1 cut(s) 533
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 4 cut(s) 133, 197, 294, 412
AgsI TTSAA 4 cut(s) 104, 439, 567, 628
AjnI CCWGG 2 cut(s) 460, 593
AleI CACNNNNGTG 1 cut(s) 60
AluBI AGCT 2 cut(s) 207, 259
AluI AGCT 2 cut(s) 207, 259
Alw21I GWGCWC 1 cut(s) 261
Alw26I GTCTC 2 cut(s) 76, 147
AlwI GGATC 2 cut(s) 593, 606
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 2 cut(s) 136, 455
ApaI GGGCCC 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 153
ArsI GACNNNNNNTTYG 2 cut(s) 523, 555
AseI ATTAAT 1 cut(s) 666
Asp718I GGTACC 1 cut(s) 217
AspS9I GGNCC 4 cut(s) 136, 137, 247, 455
AsuHPI GGTGA 1 cut(s) 337
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 1 cut(s) 247
BaeGI GKGCMC 1 cut(s) 140
BamHI GGATCC 1 cut(s) 598
BanI GGYRCC 1 cut(s) 217
BanII GRGCYC 2 cut(s) 140, 261
Bbv12I GWGCWC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 165
BccI CCATC 3 cut(s) 170, 300, 611
BcgI CGANNNNNNTGC 2 cut(s) 75, 109
BciT130I CCWGG 2 cut(s) 462, 595
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 76, 147
BfaI CTAG 1 cut(s) 660
BisI GCNGC 1 cut(s) 154
BlpI GCTNAGC 1 cut(s) 29
BlsI GCNGC 1 cut(s) 155
Bme1390I CCNGG 2 cut(s) 462, 595
Bme18I GGWCC 1 cut(s) 247
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 4 cut(s) 136, 137, 247, 455
BmiI GGNNCC 7 cut(s) 137, 138, 188, 219, 248, 523, 600
BmrFI CCNGG 2 cut(s) 462, 595
BpmI CTGGAG 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 411
Bpu1102I GCTNAGC 1 cut(s) 29
BsaJI CCNNGG 1 cut(s) 594
Bsc4I CCNNNNNNNGG 4 cut(s) 133, 197, 294, 412
Bse1I ACTGG 2 cut(s) 202, 523
BseBI CCWGG 2 cut(s) 462, 595
BseDI CCNNGG 1 cut(s) 594
BseGI GGATG 3 cut(s) 276, 311, 590
BseLI CCNNNNNNNGG 4 cut(s) 133, 197, 294, 412
BseMII CTCAG 1 cut(s) 396
BseNI ACTGG 2 cut(s) 202, 523
BseSI GKGCMC 1 cut(s) 140
BseXI GCAGC 1 cut(s) 165
BseYI CCCAGC 1 cut(s) 207
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 2 cut(s) 138, 457
BshNI GGYRCC 1 cut(s) 217
BsiHKAI GWGCWC 1 cut(s) 261
BsiHKCI CYCGRG 1 cut(s) 431
BslI CCNNNNNNNGG 4 cut(s) 133, 197, 294, 412
BsmAI GTCTC 2 cut(s) 76, 147
BsmBI CGTCTC 2 cut(s) 76, 147
BsnI GGCC 2 cut(s) 138, 457
BsoBI CYCGRG 1 cut(s) 431
Bsp120I GGGCCC 1 cut(s) 136
Bsp1286I GDGCHC 2 cut(s) 140, 261
Bsp143I GATC 3 cut(s) 121, 229, 598
Bsp1720I GCTNAGC 1 cut(s) 29
BspACI CCGC 1 cut(s) 303
BspANI GGCC 2 cut(s) 138, 457
BspCNI CTCAG 1 cut(s) 395
BspHI TCATGA 1 cut(s) 511
BspLI GGNNCC 7 cut(s) 137, 138, 188, 219, 248, 523, 600
BspPI GGATC 2 cut(s) 593, 606
BspT107I GGYRCC 1 cut(s) 217
BsrI ACTGG 2 cut(s) 202, 523
BssECI CCNNGG 1 cut(s) 594
BssMI GATC 3 cut(s) 121, 229, 598
Bst2UI CCWGG 2 cut(s) 462, 595
BstDEI CTNAG 3 cut(s) 29, 382, 411
BstENI CCTNNNNNAGG 1 cut(s) 410
BstF5I GGATG 3 cut(s) 276, 311, 590
BstKTI GATC 3 cut(s) 124, 232, 601
BstMAI GTCTC 2 cut(s) 76, 147
BstMBI GATC 3 cut(s) 121, 229, 598
BstNI CCWGG 2 cut(s) 462, 595
BstSCI CCNGG 2 cut(s) 460, 593
BstSLI GKGCMC 1 cut(s) 140
BstV1I GCAGC 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 2 cut(s) 138, 457
BtsCI GGATG 3 cut(s) 276, 311, 590
CciI TCATGA 1 cut(s) 511
Cfr13I GGNCC 4 cut(s) 136, 137, 247, 455
Csp6I GTAC 1 cut(s) 218
CviAII CATG 3 cut(s) 25, 483, 512
CviJI RGCY 6 cut(s) 9, 138, 207, 259, 373, 457
CviKI_1 RGCY 6 cut(s) 9, 138, 207, 259, 373, 457
CviQI GTAC 1 cut(s) 218
DdeI CTNAG 3 cut(s) 29, 382, 411
DpnI GATC 3 cut(s) 123, 231, 600
DpnII GATC 3 cut(s) 121, 229, 598
DraIII CACNNNGTG 1 cut(s) 533
Ecl136II GAGCTC 1 cut(s) 259
Eco24I GRGCYC 2 cut(s) 140, 261
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 259
Eco88I CYCGRG 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 410
EcoO109I RGGNCCY 2 cut(s) 136, 455
EcoRII CCWGG 2 cut(s) 460, 593
EcoT38I GRGCYC 2 cut(s) 140, 261
Esp3I CGTCTC 2 cut(s) 76, 147
FaeI CATG 3 cut(s) 28, 486, 515
FatI CATG 3 cut(s) 24, 482, 511
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 1 cut(s) 154
FokI GGATG 3 cut(s) 283, 318, 577
FriOI GRGCYC 2 cut(s) 140, 261
Fsp4HI GCNGC 1 cut(s) 154
FspBI CTAG 1 cut(s) 660
GluI GCNGC 1 cut(s) 154
GsaI CCCAGC 1 cut(s) 211
GsuI CTGGAG 1 cut(s) 321
HaeIII GGCC 2 cut(s) 138, 457
Hin1II CATG 3 cut(s) 28, 486, 515
HincII GTYRAC 1 cut(s) 637
HindII GTYRAC 1 cut(s) 637
HinfI GANTC 1 cut(s) 400
HphI GGTGA 1 cut(s) 337
Hpy166II GTNNAC 2 cut(s) 345, 637
Hpy188I TCNGA 3 cut(s) 175, 265, 703
Hpy188III TCNNGA 3 cut(s) 250, 338, 512
Hpy8I GTNNAC 2 cut(s) 345, 637
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 3 cut(s) 29, 98, 442
HpyCH4V TGCA 4 cut(s) 65, 96, 153, 486
HpyF3I CTNAG 3 cut(s) 29, 382, 411
Hsp92II CATG 3 cut(s) 28, 486, 515
KpnI GGTACC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 3 cut(s) 121, 229, 598
LmnI GCTCC 1 cut(s) 340
Lsp1109I GCAGC 1 cut(s) 165
MaeI CTAG 1 cut(s) 660
MaeIII GTNAC 1 cut(s) 493
MalI GATC 3 cut(s) 123, 231, 600
MboI GATC 3 cut(s) 121, 229, 598
MboII GAAGA 3 cut(s) 82, 451, 640
MfeI CAATTG 1 cut(s) 607
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 2 cut(s) 140, 261
MluCI AATT 5 cut(s) 274, 291, 376, 607, 667
MnlI CCTC 3 cut(s) 101, 446, 468
MseI TTAA 2 cut(s) 281, 666
MslI CAYNNNNRTG 1 cut(s) 60
MspA1I CMGCKG 1 cut(s) 303
MspR9I CCNGG 2 cut(s) 462, 595
MunI CAATTG 1 cut(s) 607
MvaI CCWGG 2 cut(s) 462, 595
NdeII GATC 3 cut(s) 121, 229, 598
NlaIII CATG 3 cut(s) 28, 486, 515
NlaIV GGNNCC 7 cut(s) 137, 138, 188, 219, 248, 523, 600
NmuCI GTSAC 1 cut(s) 493
OliI CACNNNNGTG 1 cut(s) 60
PagI TCATGA 1 cut(s) 511
PfeI GAWTC 1 cut(s) 400
PfoI TCCNGGA 1 cut(s) 460
PkrI GCNGC 1 cut(s) 155
PshBI ATTAAT 1 cut(s) 666
Psp124BI GAGCTC 1 cut(s) 261
Psp6I CCWGG 2 cut(s) 460, 593
PspFI CCCAGC 1 cut(s) 207
PspGI CCWGG 2 cut(s) 460, 593
PspN4I GGNNCC 7 cut(s) 137, 138, 188, 219, 248, 523, 600
PspOMI GGGCCC 1 cut(s) 136
PspPI GGNCC 4 cut(s) 136, 137, 247, 455
PsuI RGATCY 1 cut(s) 598
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
RseI CAYNNNNRTG 1 cut(s) 60
SacI GAGCTC 1 cut(s) 261
SaqAI TTAA 2 cut(s) 281, 666
SatI GCNGC 1 cut(s) 154
Sau3AI GATC 3 cut(s) 121, 229, 598
Sau96I GGNCC 4 cut(s) 136, 137, 247, 455
ScrFI CCNGG 2 cut(s) 462, 595
SduI GDGCHC 2 cut(s) 140, 261
SetI ASST 4 cut(s) 21, 209, 223, 261
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 60
Sse9I AATT 5 cut(s) 274, 291, 376, 607, 667
SsiI CCGC 1 cut(s) 303
SspI AATATT 1 cut(s) 90
SspMI CTAG 1 cut(s) 660
SstI GAGCTC 1 cut(s) 261
StyD4I CCNGG 2 cut(s) 460, 593
TaqI TCGA 1 cut(s) 446
TaqII GACCGA 1 cut(s) 302
TasI AATT 5 cut(s) 274, 291, 376, 607, 667
TfiI GAWTC 1 cut(s) 400
Tru1I TTAA 2 cut(s) 281, 666
Tru9I TTAA 2 cut(s) 281, 666
TseFI GTSAC 1 cut(s) 493
TseI GCWGC 1 cut(s) 153
Tsp45I GTSAC 1 cut(s) 493
TspDTI ATGAA 2 cut(s) 287, 490
VpaK11BI GGWCC 1 cut(s) 247
VspI ATTAAT 1 cut(s) 666
XagI CCTNNNNNAGG 1 cut(s) 410
XcmI CCANNNNNNNNNTGG 1 cut(s) 355
XspI CTAG 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.