Rorug05G0574100

Belongs to the glycosyl hydrolase 13 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0017595
Physical Location & Seq
Reverse (-)
76917429 .. 76920868
3440 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0574100.1

Sequence Viewer

Length: 3354 bp
ATGGCTTTGAGCTCCCAAACAGCCTCTACATCTCTTCCTTCATCATCAGCTCCTCGGTTGAAGCATGATGTGTTTCTCAGTTTTAGGGGTGAAGACACTCGCAAGGGTATTGTCTCCTATTTATACCATGAATTCCAAAACAGAGGAATACAAACATTCATGGATGACCAAGGGCTTCAGAGAGGGATGCCTATTTCTCCAAGTCTCCTAGCTGCAATAGAAGAATCGAGGTATGCAATTGTTCTTCTGTCTCCAAACTATGCTTCTTCTACATGGTGTTTGGATGAACTTGCAAAGATTCTTCAATGCTCTGAAGCCAAGAAGACACCAGTTCTGCCAATTTTTTATTATGTGGATCCCTCTGATGTGCGAAATCAAAGAGGAAGTTTTGCAGAAGCCTTCACTAAGCATGAAGAAAAGTTTAGGCAAGGCAATGACAACATAGAGAAGGTGAAGAAGTGGAGAGCTGCTTTAACGAAAGTGGCCAATATATCAGGGTTGGATTTACACAAATATGGGTCTGAAAGAGAACTTGTCCAGTGCATTGTGGAACTTGTGTGCAGTAATGTACGGCCTATTTTCATGACATCGAGTGGGGACTTTGAAGCATTTGAAGCAACAAGACAAGCCATGATTGAGCTCATGAAGGCATTACAAAATGATGAGATCGCTGTCATTGGAGTCTATGGAATGGGAGGGGTTGGGAAGACAACCATGGTCAAACATGTCGGCACACAAGCCCAAGAAAATGGTCTTTTTCATCATGTGATTATGGCTGTTGTATCCCAAAAACCTGACTTGAAAAGAATTCAAGGCGCACTTGCAGATCTGCTGGGCTTGAAACTGGAGGAGGAGTCAGAAATTGGACGAGCCAGTAGATTGCAGAAGGAGATACTTAGAAGAAATAAGATATGTATAATCTTAGACGACATATGGGATAGAATAGATTTTTCAAGCATTGGAATTCCCAGCTACAACGAGCTTCAGAGATGCAGTTCCAAAGTTCTACTAACCACCAGGCGAGTGAATGTATGCCATACCATGGAGAGCCAAGCAAACATTCATCTCAGTATCTTATCAGAAGAAGATGCTTGGAATTTGTTTGTGAAGAAAGCAAGAAAGTCTTTTGAAAAATCCACTAATTTCTATGATGTAGCGACAAAGGTAGCTAGAGAATGTGCTGGTCTCCCAATTGCGTTGATAGCAGTTGCGAGGGCACTTGGAGATAAAGATTTTGAAGAATGGAAAGAAGCAGCTCGACGCCTTGAGATGTCACAACGTGCCAACCTTGATCAGAAAGATGTGTTCAACTGTATAAAGTTAAGCTATGATTACTTGGACGGTAACGATGCCAAGTCTTGCTTCTTGCTTTGCTGCCTATTCCCAGAAGATTATGACATTCAATTGGAAGACTTGATGATATATGGGTTTGGAAAAGGATTGTTTCGAGATGTTAACAGCACACTAGAAAATGCCAGAGTCGTAGCAAATTCGGTTACTAAATACCTTAAAGCTTCTTGTTTGCTTTTGGACGGTACAAAGAATGGATGCGTAAGGATGCATGATGTCATTCGAGATGTGGCTATGTCAATTGCCTTGTCTGTAGATGGCCTCACCTTTTTGGTAAAAACTCGCTGTGAATTAAAAGATTGGCCAACCGATGCTCACGATGGCTACTCAGCAATCTCACTAATGGAAAGCAACATTTGCAAGCTTCCCAACAAGTTGGTATGTCCAAAACTTCAGATTTTATTGCTACAACGAAATGCTGATATAAGAAAGATCCCAAAAACCTTTTTTCAAAAACGGAATGATTTAAGAGTTTTAGATCTTAGGGGGACTAAAATTTCTTCACTGTCCCCATCATTCAATATCCTAACCAGACTCCAAGCTTTGCATTTAGATAATTGCTGGTCCATGATTGACATATCCATGCTTAGAAAACTAAAGAGTCTTGAGATTCTAAGTATGAGAAATTGTCATATTGAGAAATTTCCAAAAGAGATAGGAAATTTGACCAATTTAAGAATGTTGGACTTGACTGGAAATCGTGTTAAGACAGTTCCATCTCAAGTAATATCTAGGTTGCATAGACTAGAAGAGCTATACATGCAATGCAATTTTGGTGACTGGGGCAGTAAATTAGCGGGAGGTTTATTATGTTCTCTGGGTAAGAATGCTGACTTTGATGAGTTAACAGGCCTGTCACGTCTAAACAATTTGGAGGTTTCCGTAGCTGGTGTAGAATGCTTCCCTCAAAATGTTGAGTTCGATCCAAATTGGGTCTACTTTGATATATGTATCAGTCGACATGAGTCAGCCAGATGGCCGAGAATCCCATCTCCGCAAATTGACTTTCCACATCATCATTCAAGAGCATTGACCCTTAATACAACCATGAAAACCTTACCAGATTGGTTTATCGACGTGGTGACAGAGAAAACGGAAGAACTATGGTACACAGATTGCAATGGTTTAAATAACATTCTTGAGGAACATGACCGTGGGAGATTACATGGACTCAAGTCTCTCTCTATAGATGTTGCAGGGCGCCAATACAATTCGAAAGTGCTGTTGAACACAATAACATGGCCTAGGGTGTCAAATGAACCTGTGTTTGAGAACTTGGAAACATTGTACGTCTCCGTGCATTGCTTGAAGGAGTTATGTGTTGGTGAATTACCACCTGGGTCTCTGCGCAATCTTAAGACATTGAAGGTGGAAATTGGTGACATGGTGAAACCACTTTTACCGTCAAACTTATTGCACAGACTACAGAGTTTGGAAAAACTATATTGTTGTTTTATGCATAAAATGGAATATGTGTTTGGATTTGAAGAGCATCAACCACAAGAAGTTATCTTTACAAAGTTGAAAGTGATTCTATTGAGATATGTGCACAATCTAATAAACATATGGAATGGTCCTGCTCCACATGGAATCTTCAACAGTCTCAAAATATTCTACTTTTCTGATTGCCGGAAACTGATATACCTCTTCACATCTAACGTTGCTCGATGTCTTTTGCAATTGAAAGACCTCTGGGTAGAGAGTTGTTATAGATTGGACAGACTAATTGAAGCAAGCGAGGAAACAGGGAGCCGTAAGATCATTTTTCCTCAGTTGAAGAACTTAGTTTTGAGACATCTTCCAGAGCTCAAAAGGTTGTGCAGTAGTTGCAGCGCTACCATTGACATTGGGATCGAGTGCCCTTCACTGGAACACTTGTACGTAGATGAGTGCCCCCAATTTTCAGTATCTGCTTCTCAGTTTCACTACAGCAACCAAGTCCAACTCAATGATCACCGACATTTGTACACTCTACAAGGAAGTATCTTACATCCACACTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00500 ko01100 ko04973 map00500 map01100 map04973
Pfam Domains
Protein Families
Alpha-amyl_C2 Alpha-amylase

Protein Analysis

1117

Amino Acids

127.26

Weight (kDa)

7.48

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 192 2.9e-51 TIR domain
TIR_2 PF13676 24 - 120 7.9e-16 TIR domain
NB-ARC PF00931 211 - 373 4e-31 NB-ARC domain
LRR_8 PF13855 579 - 637 7.7e-08 Leucine rich repeat
LRR_14 PF23598 585 - 658 5.7e-06 Leucine-rich repeat region
LRR_14 PF23598 642 - 705 6e-10 Leucine-rich repeat region
LRR_8 PF13855 651 - 707 2.1e-06 Leucine rich repeat
LRR_RPS2 PF23247 876 - 1019 5.8e-22 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017595)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06030
malus_domestica MD00G1215700.v1.1 MD15G1083100.v1.1
prunus_persica Prupe.8G125300_v2.0.a1
pyrus_communis pycom05g07400 pycom05g07450
rosa_chinensis RchiOBHm_Chr6g0257211 RchiOBHm_Chr6g0257321
rosa_rugosa Rorug05G0574100
rosa_samantha Rh6AG092000 Rh6CG079700 Rh6DG075200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2699
AccB1I GGYRCC 1 cut(s) 2550
AccB7I CCANNNNNTGG 1 cut(s) 1042
AccI GTMKAC 2 cut(s) 2286, 2308
AciI CCGC 2 cut(s) 2147, 2345
AclI AACGTT 1 cut(s) 3009
AclWI GGATC 5 cut(s) 350, 363, 1777, 2267, 3209
AcoI YGGCCR 3 cut(s) 483, 1652, 2327
AcsI RAATTY 8 cut(s) 131, 807, 963, 1096, 1489, 1845, 1991, 2011
AcuI CTGAAG 4 cut(s) 161, 333, 968, 1727
AcyI GRCGYC 2 cut(s) 1261, 2551
AdeI CACNNNGTG 1 cut(s) 1280
AfaI GTAC 6 cut(s) 570, 1537, 2459, 2639, 3230, 3317
AfeI AGCGCT 1 cut(s) 3184
AfiI CCNNNNNNNGG 2 cut(s) 1042, 3217
AflII CTTAAG 1 cut(s) 2705
AflIII ACRYGT 1 cut(s) 724
AjiI CACGTC 2 cut(s) 2210, 2428
AjnI CCWGG 2 cut(s) 1016, 2686
AjuI GAANNNNNNNTTGG 8 cut(s) 247, 279, 1044, 1076, 1346, 1378, 2811, 2843
Alw21I GWGCWC 4 cut(s) 14, 642, 2901, 3159
Alw26I GTCTC 9 cut(s) 118, 209, 255, 1190, 2532, 2647, 2697, 2957, 3136
Alw44I GTGCAC 1 cut(s) 2897
AlwI GGATC 5 cut(s) 350, 363, 1777, 2267, 3209
AlwNI CAGNNNCTG 1 cut(s) 3260
Aor51HI AGCGCT 1 cut(s) 3184
AoxI GGCC 7 cut(s) 483, 572, 1609, 1652, 2200, 2327, 2591
ApaLI GTGCAC 1 cut(s) 2897
ApeKI GCWGC 5 cut(s) 212, 467, 1253, 1374, 3180
ApoI RAATTY 8 cut(s) 131, 807, 963, 1096, 1489, 1845, 1991, 2011
AspA2I CCTAGG 1 cut(s) 2594
AspLEI GCGC 4 cut(s) 818, 2553, 2700, 3185
AspS9I GGNCC 2 cut(s) 1914, 2924
AsuHPI GGTGA 9 cut(s) 101, 463, 1606, 2138, 2443, 2687, 2741, 2749, 3296
AsuII TTCGAA 1 cut(s) 2564
AvaII GGWCC 2 cut(s) 1914, 2924
AvrII CCTAGG 1 cut(s) 2594
BaeGI GKGCMC 4 cut(s) 1219, 2901, 3212, 3245
BalI TGGCCA 2 cut(s) 485, 1654
BamHI GGATCC 1 cut(s) 355
BanI GGYRCC 1 cut(s) 2550
BanII GRGCYC 3 cut(s) 14, 642, 3159
BbsI GAAGAC 4 cut(s) 99, 329, 713, 1416
Bbv12I GWGCWC 4 cut(s) 14, 642, 2901, 3159
BbvI GCAGC 5 cut(s) 199, 454, 1265, 1361, 3192
BccI CCATC 6 cut(s) 1601, 1664, 1870, 2074, 2319, 2347
BceAI ACGGC 2 cut(s) 587, 3087
BciT130I CCWGG 2 cut(s) 1018, 2688
BciVI GTATCC 1 cut(s) 793
BclI TGATCA 2 cut(s) 1291, 3301
BcoDI GTCTC 9 cut(s) 118, 209, 255, 1190, 2532, 2647, 2697, 2957, 3136
BfaI CTAG 6 cut(s) 209, 1170, 1466, 2082, 2096, 2595
BfmI CTRYAG 4 cut(s) 1602, 2535, 2774, 3277
BfoI RGCGCY 2 cut(s) 2554, 3186
BfrI CTTAAG 1 cut(s) 2705
BfuI GTATCC 1 cut(s) 793
BglII AGATCT 2 cut(s) 826, 1828
BisI GCNGC 5 cut(s) 213, 468, 1254, 1375, 3181
BlnI CCTAGG 1 cut(s) 2594
BlsI GCNGC 5 cut(s) 214, 469, 1255, 1376, 3182
Bme1390I CCNGG 2 cut(s) 1018, 2688
Bme18I GGWCC 2 cut(s) 1914, 2924
BmgBI CACGTC 2 cut(s) 2210, 2428
BmgT120I GGNCC 2 cut(s) 1914, 2924
BmiI GGNNCC 3 cut(s) 357, 2552, 3101
BmrFI CCNGG 2 cut(s) 1018, 2688
BmrI ACTGGG 1 cut(s) 2140
BmsI GCATC 8 cut(s) 177, 980, 1078, 1339, 1538, 1548, 1651, 2851
BmuI ACTGGG 1 cut(s) 2140
BoxI GACNNNNGTC 2 cut(s) 2314, 2524
BpiI GAAGAC 4 cut(s) 99, 329, 713, 1416
BpmI CTGGAG 1 cut(s) 866
Bpu14I TTCGAA 1 cut(s) 2564
BpuEI CTTGAG 5 cut(s) 1286, 1976, 2055, 2507, 2510
BsaAI YACGTR 1 cut(s) 3232
BsaHI GRCGYC 2 cut(s) 1261, 2551
BsaI GGTCTC 2 cut(s) 1190, 2697
BsaJI CCNNGG 7 cut(s) 53, 169, 714, 1041, 2503, 2594, 2687
BsaXI ACNNNNNCTCC 2 cut(s) 839, 869
Bsc4I CCNNNNNNNGG 2 cut(s) 1042, 3217
Bse1I ACTGG 7 cut(s) 329, 538, 849, 873, 2047, 2135, 3222
Bse3DI GCAATG 4 cut(s) 439, 2120, 2476, 2650
BseBI CCWGG 2 cut(s) 1018, 2688
BseDI CCNNGG 7 cut(s) 53, 169, 714, 1041, 2503, 2594, 2687
BseGI GGATG 6 cut(s) 169, 192, 289, 1553, 1563, 3340
BseLI CCNNNNNNNGG 2 cut(s) 1042, 3217
BseMI GCAATG 4 cut(s) 439, 2120, 2476, 2650
BseMII CTCAG 5 cut(s) 91, 1081, 1692, 3134, 3281
BseNI ACTGG 7 cut(s) 329, 538, 849, 873, 2047, 2135, 3222
BseRI GAGGAG 3 cut(s) 42, 863, 866
BseSI GKGCMC 4 cut(s) 1219, 2901, 3212, 3245
BseXI GCAGC 5 cut(s) 199, 454, 1265, 1361, 3192
BseYI CCCAGC 2 cut(s) 832, 968
BsgI GTGCAG 2 cut(s) 580, 3190
BshFI GGCC 7 cut(s) 485, 574, 1611, 1654, 2202, 2329, 2593
BshNI GGYRCC 1 cut(s) 2550
BsiHKAI GWGCWC 4 cut(s) 14, 642, 2901, 3159
BsiSI CCGG 1 cut(s) 2980
BslFI GGGAC 3 cut(s) 611, 1843, 1852
BslI CCNNNNNNNGG 2 cut(s) 1042, 3217
BsmAI GTCTC 9 cut(s) 118, 209, 255, 1190, 2532, 2647, 2697, 2957, 3136
BsmBI CGTCTC 1 cut(s) 2647
BsmFI GGGAC 3 cut(s) 611, 1843, 1852
BsmI GAATGC 2 cut(s) 2182, 2252
BsnI GGCC 7 cut(s) 485, 574, 1611, 1654, 2202, 2329, 2593
Bso31I GGTCTC 2 cut(s) 1190, 2697
Bsp119I TTCGAA 1 cut(s) 2564
Bsp1286I GDGCHC 7 cut(s) 14, 642, 1219, 2901, 3159, 3212, 3245
Bsp1407I TGTACA 1 cut(s) 3315
Bsp19I CCATGG 2 cut(s) 714, 1041
BspACI CCGC 2 cut(s) 2147, 2345
BspANI GGCC 7 cut(s) 485, 574, 1611, 1654, 2202, 2329, 2593
BspCNI CTCAG 5 cut(s) 90, 1080, 1691, 3133, 3280
BspHI TCATGA 2 cut(s) 582, 642
BspLI GGNNCC 3 cut(s) 357, 2552, 3101
BspPI GGATC 5 cut(s) 350, 363, 1777, 2267, 3209
BspQI GCTCTTC 2 cut(s) 2094, 2832
BspT104I TTCGAA 1 cut(s) 2564
BspT107I GGYRCC 1 cut(s) 2550
BspTI CTTAAG 1 cut(s) 2705
BspTNI GGTCTC 2 cut(s) 1190, 2697
BsrDI GCAATG 4 cut(s) 439, 2120, 2476, 2650
BsrGI TGTACA 1 cut(s) 3315
BsrI ACTGG 7 cut(s) 329, 538, 849, 873, 2047, 2135, 3222
BssECI CCNNGG 7 cut(s) 53, 169, 714, 1041, 2503, 2594, 2687
BssNI GRCGYC 2 cut(s) 1261, 2551
BssT1I CCWWGG 4 cut(s) 169, 714, 1041, 2594
Bst2UI CCWGG 2 cut(s) 1018, 2688
Bst4CI ACNGT 8 cut(s) 1313, 1343, 1535, 1857, 2062, 2504, 2754, 2951
Bst6I CTCTTC 4 cut(s) 39, 2094, 2832, 3002
BstACI GRCGYC 2 cut(s) 1261, 2551
BstAFI CTTAAG 1 cut(s) 2705
BstAPI GCANNNNNTGC 2 cut(s) 1707, 3177
BstAUI TGTACA 1 cut(s) 3315
BstBAI YACGTR 1 cut(s) 3232
BstBI TTCGAA 1 cut(s) 2564
BstC8I GCNNGC 2 cut(s) 1712, 3085
BstDSI CCRYGG 3 cut(s) 714, 1041, 2503
BstF5I GGATG 6 cut(s) 169, 192, 289, 1553, 1563, 3340
BstH2I RGCGCY 2 cut(s) 2554, 3186
BstHHI GCGC 4 cut(s) 818, 2553, 2700, 3185
BstMAI GTCTC 9 cut(s) 118, 209, 255, 1190, 2532, 2647, 2697, 2957, 3136
BstMWI GCNNNNNNNGC 5 cut(s) 614, 1202, 1707, 2110, 3177
BstNI CCWGG 2 cut(s) 1018, 2688
BstNSI RCATGY 2 cut(s) 728, 2113
BstPAI GACNNNNGTC 2 cut(s) 2314, 2524
BstSCI CCNGG 2 cut(s) 1016, 2686
BstSFI CTRYAG 4 cut(s) 1602, 2535, 2774, 3277
BstSLI GKGCMC 4 cut(s) 1219, 2901, 3212, 3245
BstSNI TACGTA 1 cut(s) 3232
BstV1I GCAGC 5 cut(s) 199, 454, 1265, 1361, 3192
BstV2I GAAGAC 4 cut(s) 99, 329, 713, 1416
BstX2I RGATCY 4 cut(s) 355, 826, 1782, 1828
BstXI CCANNNNNNTGG 2 cut(s) 749, 1726
BstYI RGATCY 4 cut(s) 355, 826, 1782, 1828
BsuI GTATCC 1 cut(s) 793
BsuRI GGCC 7 cut(s) 485, 574, 1611, 1654, 2202, 2329, 2593
BtgI CCRYGG 3 cut(s) 714, 1041, 2503
BtrI CACGTC 2 cut(s) 2210, 2428
BtsCI GGATG 6 cut(s) 169, 192, 289, 1553, 1563, 3340
BtsIMutI CAGTG 3 cut(s) 545, 1853, 3215
Cac8I GCNNGC 2 cut(s) 1712, 3085
CaiI CAGNNNCTG 1 cut(s) 3260
CciI TCATGA 2 cut(s) 582, 642
CfoI GCGC 4 cut(s) 818, 2553, 2700, 3185
Cfr13I GGNCC 2 cut(s) 1914, 2924
CseI GACGC 1 cut(s) 1269
Csp6I GTAC 6 cut(s) 569, 1536, 2458, 2638, 3229, 3316
CviQI GTAC 6 cut(s) 569, 1536, 2458, 2638, 3229, 3316
DinI GGCGCC 1 cut(s) 2552
DraI TTTAAA 1 cut(s) 2478
DraIII CACNNNGTG 1 cut(s) 1280
EaeI YGGCCR 3 cut(s) 483, 1652, 2327
Eam1104I CTCTTC 4 cut(s) 39, 2094, 2832, 3002
EarI CTCTTC 4 cut(s) 39, 2094, 2832, 3002
Ecl136II GAGCTC 3 cut(s) 12, 640, 3157
Eco105I TACGTA 1 cut(s) 3232
Eco130I CCWWGG 4 cut(s) 169, 714, 1041, 2594
Eco147I AGGCCT 1 cut(s) 2202
Eco24I GRGCYC 3 cut(s) 14, 642, 3159
Eco31I GGTCTC 2 cut(s) 1190, 2697
Eco47I GGWCC 2 cut(s) 1914, 2924
Eco47III AGCGCT 1 cut(s) 3184
Eco53kI GAGCTC 3 cut(s) 12, 640, 3157
Eco57I CTGAAG 4 cut(s) 161, 333, 968, 1727
EcoICRI GAGCTC 3 cut(s) 12, 640, 3157
EcoRI GAATTC 3 cut(s) 131, 807, 963
EcoRII CCWGG 2 cut(s) 1016, 2686
EcoT14I CCWWGG 4 cut(s) 169, 714, 1041, 2594
EcoT22I ATGCAT 2 cut(s) 1563, 2811
EcoT38I GRGCYC 3 cut(s) 14, 642, 3159
EgeI GGCGCC 1 cut(s) 2552
EheI GGCGCC 1 cut(s) 2552
ErhI CCWWGG 4 cut(s) 169, 714, 1041, 2594
Esp3I CGTCTC 1 cut(s) 2647
FalI AAGNNNNNCTT 6 cut(s) 804, 836, 1108, 1140, 1346, 1378
FaqI GGGAC 3 cut(s) 611, 1843, 1852
FauI CCCGC 1 cut(s) 2140
FauNDI CATATG 2 cut(s) 932, 2915
FbaI TGATCA 2 cut(s) 1291, 3301
FblI GTMKAC 2 cut(s) 2286, 2308
Fnu4HI GCNGC 5 cut(s) 213, 468, 1254, 1375, 3181
FokI GGATG 6 cut(s) 176, 199, 296, 1560, 1570, 3327
FriOI GRGCYC 3 cut(s) 14, 642, 3159
Fsp4HI GCNGC 5 cut(s) 213, 468, 1254, 1375, 3181
FspBI CTAG 6 cut(s) 209, 1170, 1466, 2082, 2096, 2595
FspI TGCGCA 1 cut(s) 2699
GlaI GCGC 4 cut(s) 817, 2552, 2699, 3184
GluI GCNGC 5 cut(s) 213, 468, 1254, 1375, 3181
GsaI CCCAGC 2 cut(s) 836, 972
GsuI CTGGAG 1 cut(s) 866
HaeII RGCGCY 2 cut(s) 2554, 3186
HaeIII GGCC 7 cut(s) 485, 574, 1611, 1654, 2202, 2329, 2593
HapII CCGG 1 cut(s) 2980
HgaI GACGC 1 cut(s) 1269
HhaI GCGC 4 cut(s) 818, 2553, 2700, 3185
Hin1I GRCGYC 2 cut(s) 1261, 2551
Hin6I GCGC 4 cut(s) 816, 2551, 2698, 3183
HinP1I GCGC 4 cut(s) 816, 2551, 2698, 3183
HincII GTYRAC 3 cut(s) 1456, 2196, 2309
HindII GTYRAC 3 cut(s) 1456, 2196, 2309
HindIII AAGCTT 3 cut(s) 1512, 1712, 1890
HpaI GTTAAC 2 cut(s) 1456, 2196
HpaII CCGG 1 cut(s) 2980
HphI GGTGA 9 cut(s) 101, 463, 1606, 2138, 2443, 2687, 2741, 2749, 3296
Hpy166II GTNNAC 7 cut(s) 1456, 2196, 2287, 2309, 2460, 2899, 3318
Hpy188III TCNNGA 9 cut(s) 583, 643, 1448, 1574, 1667, 1955, 2373, 2489, 3152
Hpy8I GTNNAC 7 cut(s) 1456, 2196, 2287, 2309, 2460, 2899, 3318
Hpy99I CGWCG 2 cut(s) 1263, 2429
HpyAV CCTTC 8 cut(s) 48, 409, 442, 640, 880, 2653, 2710, 3222
HpyCH4III ACNGT 8 cut(s) 1313, 1343, 1535, 1857, 2062, 2504, 2754, 2951
HpyCH4IV ACGT 6 cut(s) 1279, 2209, 2427, 2640, 3009, 3231
HpyF10VI GCNNNNNNNGC 5 cut(s) 614, 1202, 1707, 2110, 3177
HpySE526I ACGT 6 cut(s) 1279, 2209, 2427, 2640, 3009, 3231
Hsp92I GRCGYC 2 cut(s) 1261, 2551
HspAI GCGC 4 cut(s) 816, 2551, 2698, 3183
KasI GGCGCC 1 cut(s) 2550
Ksp22I TGATCA 2 cut(s) 1291, 3301
KspAI GTTAAC 2 cut(s) 1456, 2196
LguI GCTCTTC 2 cut(s) 2094, 2832
LmnI GCTCC 4 cut(s) 17, 55, 2935, 3099
Lsp1109I GCAGC 5 cut(s) 199, 454, 1265, 1361, 3192
LweI GCATC 8 cut(s) 177, 980, 1078, 1339, 1538, 1548, 1651, 2851
MaeI CTAG 6 cut(s) 209, 1170, 1466, 2082, 2096, 2595
MaeII ACGT 6 cut(s) 1279, 2209, 2427, 2640, 3009, 3231
MaeIII GTNAC 7 cut(s) 1272, 1343, 1495, 2126, 2205, 2431, 2729
MfeI CAATTG 5 cut(s) 237, 1191, 1403, 1590, 3029
MflI RGATCY 4 cut(s) 355, 826, 1782, 1828
MhlI GDGCHC 7 cut(s) 14, 642, 1219, 2901, 3159, 3212, 3245
MlsI TGGCCA 2 cut(s) 485, 1654
MluNI TGGCCA 2 cut(s) 485, 1654
Mly113I GGCGCC 1 cut(s) 2551
MlyI GAGTC 7 cut(s) 690, 863, 1488, 1878, 1960, 2324, 2514
MmeI TCCRAC 3 cut(s) 480, 2013, 3316
Mox20I TGGCCA 2 cut(s) 485, 1654
Mph1103I ATGCAT 2 cut(s) 1563, 2811
MscI TGGCCA 2 cut(s) 485, 1654
MslI CAYNNNNRTG 3 cut(s) 513, 1931, 2502
Msp20I TGGCCA 2 cut(s) 485, 1654
MspCI CTTAAG 1 cut(s) 2705
MspI CCGG 1 cut(s) 2980
MspR9I CCNGG 2 cut(s) 1018, 2688
MunI CAATTG 5 cut(s) 237, 1191, 1403, 1590, 3029
Mva1269I GAATGC 2 cut(s) 2182, 2252
MvaI CCWGG 2 cut(s) 1018, 2688
MwoI GCNNNNNNNGC 5 cut(s) 614, 1202, 1707, 2110, 3177
NarI GGCGCC 1 cut(s) 2551
NcoI CCATGG 2 cut(s) 714, 1041
NdeI CATATG 2 cut(s) 932, 2915
NlaIV GGNNCC 3 cut(s) 357, 2552, 3101
NmeAIII GCCGAG 1 cut(s) 2355
NmuCI GTSAC 5 cut(s) 1272, 2126, 2205, 2431, 2729
NsbI TGCGCA 1 cut(s) 2699
NsiI ATGCAT 2 cut(s) 1563, 2811
NspI RCATGY 2 cut(s) 728, 2113
NspV TTCGAA 1 cut(s) 2564
PagI TCATGA 2 cut(s) 582, 642
PceI AGGCCT 1 cut(s) 2202
PciI ACATGT 1 cut(s) 724
PciSI GCTCTTC 2 cut(s) 2094, 2832
PctI GAATGC 2 cut(s) 2182, 2252
PfeI GAWTC 6 cut(s) 224, 298, 1960, 2334, 2881, 2940
PflMI CCANNNNNTGG 1 cut(s) 1042
PkrI GCNGC 5 cut(s) 214, 469, 1255, 1376, 3182
PleI GAGTC 7 cut(s) 689, 862, 1487, 1878, 1959, 2323, 2514
PluTI GGCGCC 1 cut(s) 2554
PpsI GAGTC 7 cut(s) 689, 862, 1487, 1878, 1959, 2323, 2514
Ppu21I YACGTR 1 cut(s) 3232
PscI ACATGT 1 cut(s) 724
PshAI GACNNNNGTC 2 cut(s) 2314, 2524
Psp124BI GAGCTC 3 cut(s) 14, 642, 3159
Psp1406I AACGTT 1 cut(s) 3009
Psp6I CCWGG 2 cut(s) 1016, 2686
PspFI CCCAGC 2 cut(s) 832, 968
PspGI CCWGG 2 cut(s) 1016, 2686
PspN4I GGNNCC 3 cut(s) 357, 2552, 3101
PspPI GGNCC 2 cut(s) 1914, 2924
PstNI CAGNNNCTG 1 cut(s) 3260
PsuI RGATCY 4 cut(s) 355, 826, 1782, 1828
RsaI GTAC 6 cut(s) 570, 1537, 2459, 2639, 3230, 3317
RsaNI GTAC 6 cut(s) 569, 1536, 2458, 2638, 3229, 3316
RseI CAYNNNNRTG 3 cut(s) 513, 1931, 2502
SacI GAGCTC 3 cut(s) 14, 642, 3159
SalI GTCGAC 1 cut(s) 2307
SapI GCTCTTC 2 cut(s) 2094, 2832
SatI GCNGC 5 cut(s) 213, 468, 1254, 1375, 3181
Sau96I GGNCC 2 cut(s) 1914, 2924
SchI GAGTC 7 cut(s) 690, 863, 1488, 1878, 1960, 2324, 2514
ScrFI CCNGG 2 cut(s) 1018, 2688
SduI GDGCHC 7 cut(s) 14, 642, 1219, 2901, 3159, 3212, 3245
SfaNI GCATC 8 cut(s) 177, 980, 1078, 1339, 1538, 1548, 1651, 2851
SfcI CTRYAG 4 cut(s) 1602, 2535, 2774, 3277
SfoI GGCGCC 1 cut(s) 2552
SfuI TTCGAA 1 cut(s) 2564
SinI GGWCC 2 cut(s) 1914, 2924
SmiMI CAYNNNNRTG 3 cut(s) 513, 1931, 2502
SmlI CTYRAG 6 cut(s) 1265, 1955, 2070, 2489, 2522, 2705
SmoI CTYRAG 6 cut(s) 1265, 1955, 2070, 2489, 2522, 2705
SnaBI TACGTA 1 cut(s) 3232
SseBI AGGCCT 1 cut(s) 2202
SsiI CCGC 2 cut(s) 2147, 2345
SspDI GGCGCC 1 cut(s) 2550
SspI AATATT 1 cut(s) 2961
SspMI CTAG 6 cut(s) 209, 1170, 1466, 2082, 2096, 2595
SstI GAGCTC 3 cut(s) 14, 642, 3159
StuI AGGCCT 1 cut(s) 2202
StyD4I CCNGG 2 cut(s) 1016, 2686
StyI CCWWGG 4 cut(s) 169, 714, 1041, 2594
TaaI ACNGT 8 cut(s) 1313, 1343, 1535, 1857, 2062, 2504, 2754, 2951
TaiI ACGT 6 cut(s) 1282, 2212, 2430, 2643, 3012, 3234
TatI WGTACW 1 cut(s) 3315
TfiI GAWTC 6 cut(s) 224, 298, 1960, 2334, 2881, 2940
TscAI CASTG 3 cut(s) 545, 1860, 3222
TseFI GTSAC 5 cut(s) 1272, 2126, 2205, 2431, 2729
TseI GCWGC 5 cut(s) 212, 467, 1253, 1374, 3180
Tsp45I GTSAC 5 cut(s) 1272, 2126, 2205, 2431, 2729
TspGWI ACGGA 4 cut(s) 1822, 2221, 2459, 2635
TspRI CASTG 3 cut(s) 545, 1860, 3222
Van91I CCANNNNNTGG 1 cut(s) 1042
Vha464I CTTAAG 1 cut(s) 2705
VneI GTGCAC 1 cut(s) 2897
VpaK11BI GGWCC 2 cut(s) 1914, 2924
XapI RAATTY 8 cut(s) 131, 807, 963, 1096, 1489, 1845, 1991, 2011
XceI RCATGY 2 cut(s) 728, 2113
XmaJI CCTAGG 1 cut(s) 2594
XmiI GTMKAC 2 cut(s) 2286, 2308
XspI CTAG 6 cut(s) 209, 1170, 1466, 2082, 2096, 2595
Zsp2I ATGCAT 2 cut(s) 1563, 2811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.