MD15G1085000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012357
Physical Location & Seq
Forward (+)
5859995 .. 5861012
1018 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1085000.v1.1.491

Sequence Viewer

Length: 615 bp
ATGAGCATCTTTTATCACGAAGATGAACAATCACCAAATCCTTCCAAAAGATGCAAATTTCTTGCTGCTTGTCTCACCGATGCGTTTGCGAATTGCCATACCGGCAGAAAGCTTTCGGTTTCGAGCCGTGCGGAGGAGTGCTTGACAAGCGACTTTGATGAGGAACAGGAAGTTATTGTGTCTGCGATTCGAAGCCGTGCTATGGAAAAGCTGAGGCGCAAGCCGCGCAGCTTCTCAGATAGCTTTTCTTTTGTCTACTCTTCTAAATCGGGTGATTTATTCATAACACAGAAAGGAGTGGAAAAGCAGGAGGATCATGAAGACGATGAGAAAGCAGATTTTTTATCGGTTGCAAGCTGCCTTTCTTGCTGCTCAAGCGACGCTGCAAGTAGAGACGTGTTTCTTTCTGTCAAGACAAGCTTGTCGCGCTGTTCGAGCCTTAATGGGATCGAGTTTATAGATTTTCCAAAGCAGTCCATAATTCAGCAGTTCCGTCATTGTGAAGGCTGGCCGTTTGGGCTTTGCCGGAAGGCTGTGTTGCTTCCACCGCTGCCAAAGTCGCCCTCTGAGTCGTGGTTGTGGAAAAAAGGCACTAAGTTTGTTAAGATGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

23.05

Weight (kDa)

7.51

Isoelectric Point (pI)

73.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012357)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47410 AT1G47410
fragaria_vesca FvH4_2g34660 FvH4_2g34701
malus_domestica MD08G1103700.v1.1 MD15G1085000.v1.1
prunus_persica Prupe.1G438800_v2.0.a1
pyrus_communis pycom08g08510 pycom15g08030
rosa_chinensis RchiOBHm_Chr6g0307431
rosa_laevigata RLG00000009861 RLG00000010714
rosa_multiflora Rmu_sc0000031.1_g000017
rosa_roxburghii Rroxscaffold_7G00161000
rosa_rugosa Rorug06G0358600
rosa_samantha Rh6BG430500 Rh6CG484300 Rh6DG471000
rosa_wichuraiana Rw6G040900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 421
AccI GTMKAC 1 cut(s) 255
AccII CGCG 2 cut(s) 226, 427
AciI CCGC 3 cut(s) 131, 224, 548
AclWI GGATC 2 cut(s) 321, 455
AcoI YGGCCR 1 cut(s) 509
AcsI RAATTY 1 cut(s) 56
AfiI CCNNNNNNNGG 2 cut(s) 133, 202
AflIII ACRYGT 1 cut(s) 396
AjiI CACGTC 1 cut(s) 397
AluBI AGCT 6 cut(s) 112, 211, 231, 243, 357, 420
AluI AGCT 6 cut(s) 112, 211, 231, 243, 357, 420
Alw26I GTCTC 2 cut(s) 77, 387
AlwI GGATC 2 cut(s) 321, 455
AoxI GGCC 1 cut(s) 509
ApeKI GCWGC 6 cut(s) 65, 228, 357, 369, 383, 550
ApoI RAATTY 1 cut(s) 56
Asp700I GAANNNNTTC 1 cut(s) 112
AspLEI GCGC 3 cut(s) 219, 228, 429
AsuHPI GGTGA 3 cut(s) 24, 67, 284
AsuII TTCGAA 1 cut(s) 190
BbsI GAAGAC 1 cut(s) 327
BbvCI CCTCAGC 1 cut(s) 212
BbvI GCAGC 6 cut(s) 52, 240, 344, 356, 370, 537
BccI CCATC 1 cut(s) 601
BceAI ACGGC 3 cut(s) 111, 180, 496
BcgI CGANNNNNNTGC 2 cut(s) 68, 102
BcoDI GTCTC 2 cut(s) 77, 387
BglI GCCNNNNNGGC 2 cut(s) 102, 517
BisI GCNGC 7 cut(s) 66, 224, 229, 358, 370, 384, 551
BlsI GCNGC 7 cut(s) 67, 225, 230, 359, 371, 385, 552
BmgBI CACGTC 1 cut(s) 397
BmsI GCATC 3 cut(s) 15, 41, 70
BpiI GAAGAC 1 cut(s) 327
Bpu10I CCTNAGC 1 cut(s) 212
Bpu14I TTCGAA 1 cut(s) 190
BpuEI CTTGAG 1 cut(s) 358
Bsc4I CCNNNNNNNGG 2 cut(s) 133, 202
Bse118I RCCGGY 1 cut(s) 101
BseLI CCNNNNNNNGG 2 cut(s) 133, 202
BseMII CTCAG 3 cut(s) 203, 249, 558
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 6 cut(s) 52, 240, 344, 356, 370, 537
Bsh1236I CGCG 2 cut(s) 226, 427
BshFI GGCC 1 cut(s) 511
BsiSI CCGG 2 cut(s) 102, 526
BslI CCNNNNNNNGG 2 cut(s) 133, 202
BsmAI GTCTC 2 cut(s) 77, 387
BsmBI CGTCTC 1 cut(s) 387
BsnI GGCC 1 cut(s) 511
Bsp119I TTCGAA 1 cut(s) 190
Bsp143I GATC 2 cut(s) 313, 447
BspACI CCGC 3 cut(s) 131, 224, 548
BspANI GGCC 1 cut(s) 511
BspCNI CTCAG 3 cut(s) 204, 248, 559
BspFNI CGCG 2 cut(s) 226, 427
BspHI TCATGA 1 cut(s) 316
BspPI GGATC 2 cut(s) 321, 455
BspT104I TTCGAA 1 cut(s) 190
BsrFI RCCGGY 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 101
BssMI GATC 2 cut(s) 313, 447
Bst6I CTCTTC 1 cut(s) 265
BstBI TTCGAA 1 cut(s) 190
BstC8I GCNNGC 3 cut(s) 221, 355, 509
BstDEI CTNAG 4 cut(s) 212, 235, 567, 594
BstFNI CGCG 2 cut(s) 226, 427
BstHHI GCGC 3 cut(s) 219, 228, 429
BstKTI GATC 2 cut(s) 316, 450
BstMAI GTCTC 2 cut(s) 77, 387
BstMBI GATC 2 cut(s) 313, 447
BstUI CGCG 2 cut(s) 226, 427
BstV1I GCAGC 6 cut(s) 52, 240, 344, 356, 370, 537
BstV2I GAAGAC 1 cut(s) 327
BsuRI GGCC 1 cut(s) 511
BtrI CACGTC 1 cut(s) 397
Cac8I GCNNGC 3 cut(s) 221, 355, 509
CciI TCATGA 1 cut(s) 316
CfoI GCGC 3 cut(s) 219, 228, 429
Cfr10I RCCGGY 1 cut(s) 101
CseI GACGC 1 cut(s) 389
CviAII CATG 1 cut(s) 317
DdeI CTNAG 4 cut(s) 212, 235, 567, 594
DpnI GATC 2 cut(s) 315, 449
DpnII GATC 2 cut(s) 313, 447
DrdI GACNNNNNNGTC 1 cut(s) 421
DseDI GACNNNNNNGTC 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 509
Eam1104I CTCTTC 1 cut(s) 265
EarI CTCTTC 1 cut(s) 265
Esp3I CGTCTC 1 cut(s) 387
FaeI CATG 1 cut(s) 320
FaiI YATR 6 cut(s) 99, 203, 284, 318, 458, 479
FalI AAGNNNNNCTT 2 cut(s) 404, 436
FatI CATG 1 cut(s) 316
FblI GTMKAC 1 cut(s) 255
Fnu4HI GCNGC 7 cut(s) 66, 224, 229, 358, 370, 384, 551
Fsp4HI GCNGC 7 cut(s) 66, 224, 229, 358, 370, 384, 551
GlaI GCGC 3 cut(s) 218, 227, 428
GluI GCNGC 7 cut(s) 66, 224, 229, 358, 370, 384, 551
HaeIII GGCC 1 cut(s) 511
HapII CCGG 2 cut(s) 102, 526
HgaI GACGC 1 cut(s) 389
HhaI GCGC 3 cut(s) 219, 228, 429
Hin1II CATG 1 cut(s) 320
Hin6I GCGC 3 cut(s) 217, 226, 427
HinP1I GCGC 3 cut(s) 217, 226, 427
HindIII AAGCTT 2 cut(s) 110, 418
HinfI GANTC 2 cut(s) 187, 569
HpaII CCGG 2 cut(s) 102, 526
HphI GGTGA 3 cut(s) 24, 67, 284
Hpy166II GTNNAC 1 cut(s) 256
Hpy188I TCNGA 2 cut(s) 238, 568
Hpy188III TCNNGA 3 cut(s) 17, 317, 412
Hpy8I GTNNAC 1 cut(s) 256
Hpy99I CGWCG 1 cut(s) 383
HpyAV CCTTC 3 cut(s) 51, 497, 523
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 3 cut(s) 54, 353, 386
HpyF3I CTNAG 4 cut(s) 212, 235, 567, 594
HpySE526I ACGT 1 cut(s) 396
Hsp92II CATG 1 cut(s) 320
HspAI GCGC 3 cut(s) 217, 226, 427
Kzo9I GATC 2 cut(s) 313, 447
LpnPI CCDG 5 cut(s) 115, 152, 293, 493, 539
Lsp1109I GCAGC 6 cut(s) 52, 240, 344, 356, 370, 537
LweI GCATC 3 cut(s) 15, 41, 70
MaeII ACGT 1 cut(s) 396
MalI GATC 2 cut(s) 315, 449
MboI GATC 2 cut(s) 313, 447
MboII GAAGA 3 cut(s) 32, 252, 332
MluCI AATT 3 cut(s) 56, 91, 480
MlyI GAGTC 1 cut(s) 578
MnlI CCTC 5 cut(s) 127, 154, 207, 304, 574
MroXI GAANNNNTTC 1 cut(s) 112
MseI TTAA 3 cut(s) 441, 603, 613
MslI CAYNNNNRTG 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 550
MspI CCGG 2 cut(s) 102, 526
MvnI CGCG 2 cut(s) 226, 427
NdeII GATC 2 cut(s) 313, 447
NlaIII CATG 1 cut(s) 320
NspV TTCGAA 1 cut(s) 190
PagI TCATGA 1 cut(s) 316
PcsI WCGNNNNNNNCGW 1 cut(s) 431
PdmI GAANNNNTTC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 187
PkrI GCNGC 7 cut(s) 67, 225, 230, 359, 371, 385, 552
PleI GAGTC 1 cut(s) 577
PpsI GAGTC 1 cut(s) 577
RseI CAYNNNNRTG 1 cut(s) 21
SaqAI TTAA 3 cut(s) 441, 603, 613
SatI GCNGC 7 cut(s) 66, 224, 229, 358, 370, 384, 551
Sau3AI GATC 2 cut(s) 313, 447
SchI GAGTC 1 cut(s) 578
SetI ASST 7 cut(s) 114, 213, 233, 245, 359, 399, 422
SfaNI GCATC 3 cut(s) 15, 41, 70
SfuI TTCGAA 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 21
SmlI CTYRAG 1 cut(s) 373
SmoI CTYRAG 1 cut(s) 373
Sse9I AATT 3 cut(s) 56, 91, 480
SsiI CCGC 3 cut(s) 131, 224, 548
TaiI ACGT 1 cut(s) 399
TaqI TCGA 4 cut(s) 122, 190, 434, 450
TasI AATT 3 cut(s) 56, 91, 480
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 1 cut(s) 187
Tru1I TTAA 3 cut(s) 441, 603, 613
Tru9I TTAA 3 cut(s) 441, 603, 613
TseI GCWGC 6 cut(s) 65, 228, 357, 369, 383, 550
TspDTI ATGAA 3 cut(s) 39, 271, 333
TspGWI ACGGA 1 cut(s) 482
XapI RAATTY 1 cut(s) 56
XmiI GTMKAC 1 cut(s) 255
XmnI GAANNNNTTC 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.