MD15G1092000.v1.1

Protein of unknown function (DUF1677)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017191
Physical Location & Seq
Forward (+)
6383678 .. 6384232
555 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1092000.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGTTTATTATATCTTTGAGCTAATCTCGCTCTTTCATGTCCATGCATGCGTTCGCGTAAACATGCATGCATACATACAGCCGCAGACCGAGCAAAGGCTTCGAAAGGCATTCTCCGATGTTTCAGTTGAAATAAACAAGTATAAGAACGAACTGGAATTTAGTAGCAACGATTTTATGCTTGCTACAATTGATGAAGTGAAGCAGGCAGAGTGTGAATGCTGTGGACTGAAGGAGGAGTGCACACAAGGCTATATCAACGAAGTCGAGGGCTCCTTTTCAGGTAAATGGGTTTGTGGGCTCTGTTCAGTAGCTGTCAAAGATAATAAGACGAGGGCTCCAAATGGCACTCCCATGCAAGAAGTCGTGAGCTCTCACAGGGACTTTTGCCAGAAGTACAAGAGCACTAGGCTCAACCCTCAGCTCTCATTGACCTCTGCCCTGAGGGACATAGCAAAAAGAAGCTCTGAGAGTAGAAATCTGAACAACAACATGCCAATGCTTGGTCGTAGAAACAGCTGCGGTCCAAGAATTGATTTCAAGCAATATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1677

Protein Analysis

185

Amino Acids

21.11

Weight (kDa)

8.35

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1677 PF07911 67 - 158 1.4e-31 Protein of unknown function (DUF1677)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017191)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G25780
fragaria_vesca FvH4_2g35380
malus_domestica MD15G1092000.v1.1
prunus_persica Prupe.1G446900_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0306531
rosa_laevigata RLG00000010788
rosa_multiflora Rmu_sc0002971.1_g000006
rosa_rugosa Rorug06G0350100
rosa_samantha Rh6AG462400 Rh6BG438400 Rh6CG476600 Rh6DG463300
rosa_wichuraiana Rw6G040300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 503
AccII CGCG 1 cut(s) 57
AciI CCGC 2 cut(s) 83, 522
AcsI RAATTY 1 cut(s) 158
AcuI CTGAAG 1 cut(s) 251
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 2 cut(s) 96, 503
AgsI TTSAA 2 cut(s) 131, 541
AluBI AGCT 6 cut(s) 22, 314, 372, 424, 465, 519
AluI AGCT 6 cut(s) 22, 314, 372, 424, 465, 519
Alw21I GWGCWC 3 cut(s) 245, 374, 407
Alw44I GTGCAC 1 cut(s) 241
AlwNI CAGNNNCTG 1 cut(s) 314
ApaLI GTGCAC 1 cut(s) 241
ApeKI GCWGC 1 cut(s) 519
ApoI RAATTY 1 cut(s) 158
AspS9I GGNCC 1 cut(s) 524
AsuII TTCGAA 1 cut(s) 103
AvaII GGWCC 1 cut(s) 524
AxyI CCTNAGG 1 cut(s) 443
BaeGI GKGCMC 1 cut(s) 245
BanII GRGCYC 4 cut(s) 275, 303, 340, 374
Bbv12I GWGCWC 3 cut(s) 245, 374, 407
BbvCI CCTCAGC 1 cut(s) 420
BbvI GCAGC 1 cut(s) 506
BcgI CGANNNNNNTGC 2 cut(s) 83, 117
BfaI CTAG 1 cut(s) 408
BisI GCNGC 2 cut(s) 83, 520
BlsI GCNGC 2 cut(s) 84, 521
Bme18I GGWCC 1 cut(s) 524
BmgT120I GGNCC 1 cut(s) 524
BmiI GGNNCC 2 cut(s) 274, 339
BplI GAGNNNNNCTC 2 cut(s) 11, 43
Bpu10I CCTNAGC 1 cut(s) 420
Bpu14I TTCGAA 1 cut(s) 103
BsaXI ACNNNNNCTCC 2 cut(s) 322, 352
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 503
Bse1I ACTGG 1 cut(s) 159
Bse21I CCTNAGG 1 cut(s) 443
BseLI CCNNNNNNNGG 2 cut(s) 96, 503
BseMII CTCAG 3 cut(s) 434, 434, 459
BseNI ACTGG 1 cut(s) 159
BseRI GAGGAG 1 cut(s) 251
BseSI GKGCMC 1 cut(s) 245
BseXI GCAGC 1 cut(s) 506
Bsh1236I CGCG 1 cut(s) 57
BsiHKAI GWGCWC 3 cut(s) 245, 374, 407
BslFI GGGAC 2 cut(s) 395, 461
BslI CCNNNNNNNGG 2 cut(s) 96, 503
BsmFI GGGAC 2 cut(s) 395, 461
BsmI GAATGC 2 cut(s) 110, 224
Bsp119I TTCGAA 1 cut(s) 103
Bsp1286I GDGCHC 6 cut(s) 245, 275, 303, 340, 374, 407
BspACI CCGC 2 cut(s) 83, 522
BspCNI CTCAG 3 cut(s) 433, 435, 460
BspFNI CGCG 1 cut(s) 57
BspLI GGNNCC 2 cut(s) 274, 339
BspT104I TTCGAA 1 cut(s) 103
BsrI ACTGG 1 cut(s) 159
BstBI TTCGAA 1 cut(s) 103
BstC8I GCNNGC 4 cut(s) 49, 69, 183, 207
BstDEI CTNAG 3 cut(s) 420, 443, 468
BstFNI CGCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 3 cut(s) 28, 91, 249
BstNSI RCATGY 4 cut(s) 51, 67, 71, 496
BstSLI GKGCMC 1 cut(s) 245
BstUI CGCG 1 cut(s) 57
BstV1I GCAGC 1 cut(s) 506
Bsu36I CCTNAGG 1 cut(s) 443
Cac8I GCNNGC 4 cut(s) 49, 69, 183, 207
CaiI CAGNNNCTG 1 cut(s) 314
Cfr13I GGNCC 1 cut(s) 524
Csp6I GTAC 1 cut(s) 397
CviAII CATG 7 cut(s) 38, 44, 48, 64, 68, 355, 493
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 3 cut(s) 420, 443, 468
Ecl136II GAGCTC 1 cut(s) 372
Eco24I GRGCYC 4 cut(s) 275, 303, 340, 374
Eco47I GGWCC 1 cut(s) 524
Eco53kI GAGCTC 1 cut(s) 372
Eco57I CTGAAG 1 cut(s) 251
Eco81I CCTNAGG 1 cut(s) 443
EcoICRI GAGCTC 1 cut(s) 372
EcoT22I ATGCAT 3 cut(s) 49, 69, 73
EcoT38I GRGCYC 4 cut(s) 275, 303, 340, 374
FaeI CATG 7 cut(s) 41, 47, 51, 67, 71, 358, 496
FaqI GGGAC 2 cut(s) 395, 461
FatI CATG 7 cut(s) 37, 43, 47, 63, 67, 354, 492
Fnu4HI GCNGC 2 cut(s) 83, 520
FriOI GRGCYC 4 cut(s) 275, 303, 340, 374
Fsp4HI GCNGC 2 cut(s) 83, 520
FspBI CTAG 1 cut(s) 408
GluI GCNGC 2 cut(s) 83, 520
Hin1II CATG 7 cut(s) 41, 47, 51, 67, 71, 358, 496
Hpy166II GTNNAC 3 cut(s) 61, 227, 243
Hpy188I TCNGA 3 cut(s) 118, 469, 483
Hpy188III TCNNGA 1 cut(s) 367
Hpy8I GTNNAC 3 cut(s) 61, 227, 243
HpyAV CCTTC 1 cut(s) 226
HpyCH4V TGCA 5 cut(s) 47, 67, 71, 243, 358
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 91, 249
HpyF3I CTNAG 3 cut(s) 420, 443, 468
Hsp92II CATG 7 cut(s) 41, 47, 51, 67, 71, 358, 496
LmnI GCTCC 2 cut(s) 278, 343
LpnPI CCDG 6 cut(s) 140, 191, 267, 364, 404, 455
Lsp1109I GCAGC 1 cut(s) 506
MaeI CTAG 1 cut(s) 408
MfeI CAATTG 1 cut(s) 189
MhlI GDGCHC 6 cut(s) 245, 275, 303, 340, 374, 407
MluCI AATT 3 cut(s) 158, 189, 531
MnlI CCTC 6 cut(s) 229, 262, 327, 429, 438, 445
Mph1103I ATGCAT 3 cut(s) 49, 69, 73
MslI CAYNNNNRTG 3 cut(s) 42, 353, 497
MspA1I CMGCKG 1 cut(s) 519
MunI CAATTG 1 cut(s) 189
Mva1269I GAATGC 2 cut(s) 110, 224
MvnI CGCG 1 cut(s) 57
MwoI GCNNNNNNNGC 3 cut(s) 28, 91, 249
NlaIII CATG 7 cut(s) 41, 47, 51, 67, 71, 358, 496
NlaIV GGNNCC 2 cut(s) 274, 339
NsiI ATGCAT 3 cut(s) 49, 69, 73
NspI RCATGY 4 cut(s) 51, 67, 71, 496
NspV TTCGAA 1 cut(s) 103
PaeI GCATGC 2 cut(s) 51, 71
PctI GAATGC 2 cut(s) 110, 224
PflMI CCANNNNNTGG 1 cut(s) 503
PkrI GCNGC 2 cut(s) 84, 521
Psp124BI GAGCTC 1 cut(s) 374
PspN4I GGNNCC 2 cut(s) 274, 339
PspPI GGNCC 1 cut(s) 524
PstNI CAGNNNCTG 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 519
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
RseI CAYNNNNRTG 3 cut(s) 42, 353, 497
SacI GAGCTC 1 cut(s) 374
SatI GCNGC 2 cut(s) 83, 520
Sau96I GGNCC 1 cut(s) 524
SduI GDGCHC 6 cut(s) 245, 275, 303, 340, 374, 407
SetI ASST 8 cut(s) 24, 286, 316, 374, 426, 437, 467, 521
SfuI TTCGAA 1 cut(s) 103
SinI GGWCC 1 cut(s) 524
SmiMI CAYNNNNRTG 3 cut(s) 42, 353, 497
SphI GCATGC 2 cut(s) 51, 71
Sse9I AATT 3 cut(s) 158, 189, 531
SsiI CCGC 2 cut(s) 83, 522
SspMI CTAG 1 cut(s) 408
SstI GAGCTC 1 cut(s) 374
TaqI TCGA 2 cut(s) 103, 267
TaqII GACCGA 1 cut(s) 104
TasI AATT 3 cut(s) 158, 189, 531
TatI WGTACW 1 cut(s) 396
TauI GCSGC 1 cut(s) 85
TseI GCWGC 1 cut(s) 519
TspDTI ATGAA 2 cut(s) 26, 210
Van91I CCANNNNNTGG 1 cut(s) 503
VneI GTGCAC 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 524
XapI RAATTY 1 cut(s) 158
XceI RCATGY 4 cut(s) 51, 67, 71, 496
XspI CTAG 1 cut(s) 408
Zsp2I ATGCAT 3 cut(s) 49, 69, 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.