Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0007349

Physical Location & Seq

Genomic Coordinates Reverse (-)

6728452 .. 6740239

Gene Structure 11788 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1096400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2109 bp

ATGGTGAATTATATGGTGGAACGCGCCACGAGCGACATGCTTATCGGTCCTGATTGGGCCATGAACCTCGATATCTGTGACATATGCAACCACGACCCTGTGCAAGCGAAGGATGTTGTTAAAGGTATAAAGAAGCGCCTTGGAAGTAAGAATTCAAAAGTTCAACTTCTTGCCCTTACACTGTTGGAAACAATAATAAAGAATTGTGGAGACATTGTTCATATGCATGTAGCAGAGAAAGACCTCCTCCATGATATGGTTAGAATAGCAAAAAAGAAGCCGGACTATCATGTCAAAGAGAAGATATTGATCCTGATAGATACCTGGCAAGAAGCTTTTGGGGGACCGAGAGCAAGATATCCACAATACTATGCTGCATACCAGGATTTGTTGCGTTCTGGAGCAGTATTCCCTCAAAGATCTGAATCATCTGCGCCTGTATTCACACCACCCCAGACACAACCATTGTCAGCATTTCCTCAGAATTTGCAAAATCCAGATCGTCCGAGAGATGTAGCTGAGCCTTCTGCAGAGGCTGAGTTCCCAACCTTGAGCTTGACGGAAATTCAGAATGCACGGGGTATCATGGATGTCCTGGCAGAAATGCTAACTGCAATAGATAACAAAGAGGGGCTTAGACAGGAGGTGATTGTTGATTTGGTTGACCAGTGCCGAACATATAAGCAGAGGGTGGTACACCTTGTTAACTCGACTGCGGATGAATCACTTCTGTGTCAAGGGCTGGCGCTGAATGATGACTTGCAACGTCTTCTAGCCAAACATGAAGCCATTGCTTCTGGAAATCCAGTTCAGCCGGATAAACCCAAGCCTGCAGCTAACGGAGCACTTGTAAATGTTGGTGGTCCTCTAGTTGACACTGGAAATGACAGCAAACAGCCTGATGGGAGATCCGCCTCAAGTTCCGGTTCCGGTGCTAATCCACTTAATCAGTTATTGCTTACTGCCCCTCCAACTACTAATGGTTCAACTTCTACTGCAAAGGCCGATCCCAGGATGGATCTTCTTAGCGGTGATGACTTTGTCTCACCAAAGGCAGATGCTTCATTGGCTCTTGTTCCTGTGGGACAACAGCCAACAACTCCTATTAATCAGCAGAATGCCCTTGCTCTTATCGACATGTTTGGCACTGAGAATGTCCCGAACCCTGTGAACACCCATCCTACTAATGCAGCCAGCCAACCCAATCCTTCACCCCCGCAATTCCCACAGCAGCAGAATTTTCAATCTCCACAAGCAGGATTTCTCCCGAACGGAAATGGCCCAATTATGGGGGTCCCTCAGTATGAGCAGTCTCCATTCGCACATGGTGCTGCTCCTGCCTGGAATGGTCAGATGGTGCAGTCGCAACCACTGCAGCAGCAGCCGGCTTCACCAGGCTATGGTTCACCAACCAGTGGATCATTGCCACCACCTCCCTGGGAAGCTCAGTCAATAGATGATGGCAGCCCAGTAGCAGGTTCTCGATTCTCTCCACCCCCACTGCAAGTTACTACCAAGGTGGTGGTCAATTCACCAGGTGGTTTCCATCCCCAGGGACCTCATAGCGACCAGGTTGTAGGTATGTATATCCAGCCAATTACAACTACCCATTTGTCAGCAATCAATAACCAGGTTGGTCCTAGCAATCATTTGGGTTTGCCCTCTTCACCGGTCCAAGGGCCATACACGGGTATGACTCCACAAGCTATTCAAGCAGGCCAGATGACATCTATGTATCCTCAACAAATGTATGGTGGTAATCAACTAGTCCCCTACGGCTATGATCAACAGCAGCAACAGCATCAAGCATACGCCCTTCAGCAACAGCAACAGCAATATCAACAACAGCAAGCCTACGCCCTTCAGCAACAGCAGCACGCATACGCCCTTGAGCAACAGCAACAACAGCAATACGCCCTTCAGCAGCAAATGTACGGTCTTTCCGTAAGAGATGGTAATGGTATGAAAAACCCCTACCAGGTTTCTGCTCCATCTTACGTTCCACCAAGCAAGCCATCCAGGCCAGAGGATAAGCTATTCGGAGACCTCGTTGACTTTGCAAAAATGAAGCCAACAAAAACGACGCCTGGCGCAGCTGGTAGCGTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006464 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009987 GO:0015031 GO:0015833 GO:0015919 GO:0016042 GO:0016043 GO:0016054 GO:0016558 GO:0016567 GO:0016740 GO:0017038 GO:0019395 GO:0019538 GO:0019752 GO:0019787 GO:0019899 GO:0030258 GO:0031625 GO:0032446 GO:0032787 GO:0033036 GO:0033365 GO:0034440 GO:0034613 GO:0036211 GO:0042886 GO:0043170 GO:0043412 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044282 GO:0044389 GO:0044424 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055114 GO:0061630 GO:0061659 GO:0065002 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0140096 GO:1901564 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GAT VHS

Protein Analysis

703

Amino Acids

75.98

Weight (kDa)

5.68

Isoelectric Point (pI)

52.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VHS	PF00790	2 - 128	1.1e-31	VHS domain
GAT	PF03127	196 - 267	9.6e-17	GAT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007349)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G32760 AT4G32760 AT4G32760
fragaria_vesca	FvH4_2g35970 FvH4_2g35970 FvH4_2g35970 FvH4_2g35970 FvH4_2g35970
malus_domestica	MD08G1116900.v1.1 MD15G1096400.v1.1
prunus_persica	Prupe.1G451300_v2.0.a1 Prupe.1G451300_v2.0.a1
pyrus_communis	pycom08g09740 pycom15g08850
rosa_chinensis	RchiOBHm_Chr6g0305901
rosa_laevigata	RLG00000010848
rosa_multiflora	Rmu_sc0001809.1_g000047
rosa_roxburghii	Rroxscaffold_7G00162230
rosa_rugosa	Rorug06G0344900 Rorug06G0345000
rosa_samantha	Rh6AG456700 Rh6BG444600 Rh6CG470600 Rh6DG457300
rosa_wichuraiana	Rw6G039840

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	290
Acc36I	ACCTGC	1 cut(s)	1466
AccB7I	CCANNNNNTGG	3 cut(s)	256, 1400, 1415
AccII	CGCG	1 cut(s)	24
AciI	CCGC	4 cut(s)	716, 912, 1029, 1217
AclWI	GGATC	5 cut(s)	304, 903, 1001, 1026, 1426
AcsI	RAATTY	4 cut(s)	151, 484, 564, 1237
AcuI	CTGAAG	3 cut(s)	1802, 1847, 1904
AcyI	GRCGYC	1 cut(s)	2084
AdeI	CACNNNGTG	2 cut(s)	1328, 1538
AfaI	GTAC	2 cut(s)	696, 1934
AflIII	ACRYGT	1 cut(s)	1137
AgeI	ACCGGT	1 cut(s)	1669
AgsI	TTSAA	5 cut(s)	156, 164, 987, 1244, 1712
AhlI	ACTAGT	1 cut(s)	1765
AleI	CACNNNNGTG	1 cut(s)	730
AloI	GAACNNNNNNTCC	4 cut(s)	201, 233, 792, 824
AluBI	AGCT	8 cut(s)	335, 518, 555, 836, 1445, 1706, 2035, 2096
AluI	AGCT	8 cut(s)	335, 518, 555, 836, 1445, 1706, 2035, 2096
Alw21I	GWGCWC	1 cut(s)	847
Alw26I	GTCTC	4 cut(s)	204, 1048, 1317, 2037
AlwI	GGATC	5 cut(s)	304, 903, 1001, 1026, 1426
AlwNI	CAGNNNCTG	1 cut(s)	536
AoxI	GGCC	6 cut(s)	57, 1002, 1279, 1679, 1717, 2021
ApoI	RAATTY	4 cut(s)	151, 484, 564, 1237
ArsI	GACNNNNNNTTYG	2 cut(s)	1921, 1953
AseI	ATTAAT	1 cut(s)	1107
AsiGI	ACCGGT	1 cut(s)	1669
Asp700I	GAANNNNTTC	1 cut(s)	726
AspLEI	GCGC	5 cut(s)	26, 138, 436, 748, 2093
AsuHPI	GGTGA	9 cut(s)	16, 658, 1038, 1043, 1203, 1383, 1398, 1524, 1659
AvaII	GGWCC	7 cut(s)	47, 344, 863, 1294, 1556, 1637, 1672
BauI	CACGAG	1 cut(s)	28
BbsI	GAAGAC	1 cut(s)	761
Bbv12I	GWGCWC	1 cut(s)	847
BccI	CCATC	9 cut(s)	896, 1009, 1185, 1348, 1454, 1554, 1946, 1999, 2023
BceAI	ACGGC	1 cut(s)	1792
BcgI	CGANNNNNNTGC	2 cut(s)	19, 53
BciVI	GTATCC	1 cut(s)	1746
BclI	TGATCA	1 cut(s)	1783
BcoDI	GTCTC	4 cut(s)	204, 1048, 1317, 2037
BcuI	ACTAGT	1 cut(s)	1765
BfaI	CTAG	4 cut(s)	773, 869, 1641, 1766
BfmI	CTRYAG	3 cut(s)	528, 831, 1373
BfoI	RGCGCY	2 cut(s)	139, 749
BfuAI	ACCTGC	1 cut(s)	1466
BfuI	GTATCC	1 cut(s)	1746
BglI	GCCNNNNNGGC	1 cut(s)	2020
BglII	AGATCT	1 cut(s)	419
BlpI	GCTNAGC	1 cut(s)	519
Bme18I	GGWCC	7 cut(s)	47, 344, 863, 1294, 1556, 1637, 1672
BmiI	GGNNCC	5 cut(s)	345, 928, 1295, 1296, 1557
BmrI	ACTGGG	1 cut(s)	1463
BmsI	GCATC	2 cut(s)	1048, 1810
BmuI	ACTGGG	1 cut(s)	1463
BpiI	GAAGAC	1 cut(s)	761
BpmI	CTGGAG	1 cut(s)	420
Bpu1102I	GCTNAGC	1 cut(s)	519
BpuEI	CTTGAG	3 cut(s)	571, 901, 1910
BsaBI	GATNNNNATC	2 cut(s)	308, 424
BsaHI	GRCGYC	1 cut(s)	2084
BsaI	GGTCTC	1 cut(s)	2037
BsaJI	CCNNGG	8 cut(s)	139, 1010, 1436, 1437, 1515, 1551, 1552, 1675
BsaWI	WCCGGW	3 cut(s)	923, 929, 1669
BsaXI	ACNNNNNCTCC	8 cut(s)	201, 231, 834, 864, 952, 982, 2034, 2064
Bse118I	RCCGGY	2 cut(s)	1384, 1669
Bse1I	ACTGG	5 cut(s)	667, 806, 883, 1413, 1469
Bse3DI	GCAATG	2 cut(s)	789, 1421
Bse8I	GATNNNNATC	2 cut(s)	308, 424
BseDI	CCNNGG	8 cut(s)	139, 1010, 1436, 1437, 1515, 1551, 1552, 1675
BseGI	GGATG	7 cut(s)	118, 595, 724, 1020, 1177, 1546, 2015
BseJI	GATNNNNATC	2 cut(s)	308, 424
BseMI	GCAATG	2 cut(s)	789, 1421
BseMII	CTCAG	6 cut(s)	494, 510, 528, 1140, 1313, 1460
BseNI	ACTGG	5 cut(s)	667, 806, 883, 1413, 1469
BseRI	GAGGAG	1 cut(s)	236
BsgI	GTGCAG	1 cut(s)	1379
Bsh1236I	CGCG	1 cut(s)	24
BshFI	GGCC	6 cut(s)	59, 1004, 1281, 1681, 1719, 2023
BshTI	ACCGGT	1 cut(s)	1669
BsiHKAI	GWGCWC	1 cut(s)	847
BsiSI	CCGG	6 cut(s)	281, 815, 924, 930, 1385, 1670
BslFI	GGGAC	6 cut(s)	357, 1098, 1142, 1280, 1569, 1754
BsmAI	GTCTC	4 cut(s)	204, 1048, 1317, 2037
BsmFI	GGGAC	6 cut(s)	357, 1098, 1142, 1280, 1569, 1754
BsmI	GAATGC	2 cut(s)	577, 1123
BsnI	GGCC	6 cut(s)	59, 1004, 1281, 1681, 1719, 2023
Bso31I	GGTCTC	1 cut(s)	2037
Bsp1286I	GDGCHC	1 cut(s)	847
Bsp143I	GATC	8 cut(s)	309, 419, 499, 908, 1006, 1018, 1418, 1783
Bsp1720I	GCTNAGC	1 cut(s)	519
BspACI	CCGC	4 cut(s)	716, 912, 1029, 1217
BspANI	GGCC	6 cut(s)	59, 1004, 1281, 1681, 1719, 2023
BspCNI	CTCAG	6 cut(s)	493, 511, 529, 1141, 1312, 1459
BspFNI	CGCG	1 cut(s)	24
BspLI	GGNNCC	5 cut(s)	345, 928, 1295, 1296, 1557
BspMAI	CTGCAG	3 cut(s)	532, 835, 1377
BspMI	ACCTGC	1 cut(s)	1466
BspPI	GGATC	5 cut(s)	304, 903, 1001, 1026, 1426
BspTNI	GGTCTC	1 cut(s)	2037
BsrDI	GCAATG	2 cut(s)	789, 1421
BsrFI	RCCGGY	2 cut(s)	1384, 1669
BsrI	ACTGG	5 cut(s)	667, 806, 883, 1413, 1469
BssAI	RCCGGY	2 cut(s)	1384, 1669
BssECI	CCNNGG	8 cut(s)	139, 1010, 1436, 1437, 1515, 1551, 1552, 1675
BssMI	GATC	8 cut(s)	309, 419, 499, 908, 1006, 1018, 1418, 1783
BssNI	GRCGYC	1 cut(s)	2084
BssSI	CACGAG	1 cut(s)	28
BssT1I	CCWWGG	3 cut(s)	139, 1515, 1675
Bst2BI	CACGAG	1 cut(s)	28
Bst4CI	ACNGT	2 cut(s)	183, 1937
Bst6I	CTCTTC	1 cut(s)	1669
BstACI	GRCGYC	1 cut(s)	2084
BstAPI	GCANNNNNTGC	2 cut(s)	1328, 1372
BstC8I	GCNNGC	9 cut(s)	105, 744, 831, 1195, 1386, 1717, 1851, 1878, 2012
BstDEI	CTNAG	8 cut(s)	480, 519, 537, 635, 1025, 1149, 1299, 1446
BstF5I	GGATG	7 cut(s)	118, 595, 724, 1020, 1177, 1546, 2015
BstFNI	CGCG	1 cut(s)	24
BstH2I	RGCGCY	2 cut(s)	139, 749
BstHHI	GCGC	5 cut(s)	26, 138, 436, 748, 2093
BstKTI	GATC	8 cut(s)	312, 422, 502, 911, 1009, 1021, 1421, 1786
BstMAI	GTCTC	4 cut(s)	204, 1048, 1317, 2037
BstMBI	GATC	8 cut(s)	309, 419, 499, 908, 1006, 1018, 1418, 1783
BstNSI	RCATGY	3 cut(s)	40, 230, 1141
BstSFI	CTRYAG	3 cut(s)	528, 831, 1373
BstUI	CGCG	1 cut(s)	24
BstV2I	GAAGAC	1 cut(s)	761
BstX2I	RGATCY	3 cut(s)	419, 908, 1018
BstXI	CCANNNNNNTGG	2 cut(s)	1437, 1522
BstYI	RGATCY	3 cut(s)	419, 908, 1018
BsuI	GTATCC	1 cut(s)	1746
BsuRI	GGCC	6 cut(s)	59, 1004, 1281, 1681, 1719, 2023
BtsCI	GGATG	7 cut(s)	118, 595, 724, 1020, 1177, 1546, 2015
BtsI	GCAGTG	2 cut(s)	1370, 1499
BtsIMutI	CAGTG	7 cut(s)	179, 674, 876, 1146, 1370, 1420, 1499
BveI	ACCTGC	1 cut(s)	1466
Cac8I	GCNNGC	9 cut(s)	105, 744, 831, 1195, 1386, 1717, 1851, 1878, 2012
CaiI	CAGNNNCTG	1 cut(s)	536
CfoI	GCGC	5 cut(s)	26, 138, 436, 748, 2093
Cfr10I	RCCGGY	2 cut(s)	1384, 1669
CseI	GACGC	2 cut(s)	2092, 2092
CsiI	ACCWGGT	4 cut(s)	1534, 1569, 1629, 1977
Csp6I	GTAC	2 cut(s)	695, 1933
CspAI	ACCGGT	1 cut(s)	1669
CviAII	CATG	9 cut(s)	37, 61, 227, 251, 290, 586, 782, 1138, 1325
CviQI	GTAC	2 cut(s)	695, 1933
DdeI	CTNAG	8 cut(s)	480, 519, 537, 635, 1025, 1149, 1299, 1446
DpnI	GATC	8 cut(s)	311, 421, 501, 910, 1008, 1020, 1420, 1785
DpnII	GATC	8 cut(s)	309, 419, 499, 908, 1006, 1018, 1418, 1783
DraIII	CACNNNGTG	2 cut(s)	1328, 1538
DrdI	GACNNNNNNGTC	1 cut(s)	290
DseDI	GACNNNNNNGTC	1 cut(s)	290
Eam1104I	CTCTTC	1 cut(s)	1669
EarI	CTCTTC	1 cut(s)	1669
EciI	GGCGGA	1 cut(s)	901
Eco130I	CCWWGG	3 cut(s)	139, 1515, 1675
Eco31I	GGTCTC	1 cut(s)	2037
Eco32I	GATATC	2 cut(s)	73, 359
Eco47I	GGWCC	7 cut(s)	47, 344, 863, 1294, 1556, 1637, 1672
Eco57I	CTGAAG	3 cut(s)	1802, 1847, 1904
EcoO109I	RGGNCCY	2 cut(s)	1294, 1556
EcoRI	GAATTC	1 cut(s)	151
EcoRV	GATATC	2 cut(s)	73, 359
EcoT14I	CCWWGG	3 cut(s)	139, 1515, 1675
EcoT22I	ATGCAT	1 cut(s)	228
ErhI	CCWWGG	3 cut(s)	139, 1515, 1675
FaeI	CATG	9 cut(s)	40, 64, 230, 254, 293, 589, 785, 1141, 1328
FalI	AAGNNNNNCTT	4 cut(s)	150, 182, 618, 650
FaqI	GGGAC	6 cut(s)	357, 1098, 1142, 1280, 1569, 1754
FatI	CATG	9 cut(s)	36, 60, 226, 250, 289, 585, 781, 1137, 1324
FauI	CCCGC	1 cut(s)	1224
FauNDI	CATATG	2 cut(s)	83, 222
FbaI	TGATCA	1 cut(s)	1783
FokI	GGATG	7 cut(s)	125, 602, 731, 1027, 1164, 1533, 2002
FspBI	CTAG	4 cut(s)	773, 869, 1641, 1766
GlaI	GCGC	5 cut(s)	25, 137, 435, 747, 2092
GsuI	CTGGAG	1 cut(s)	420
HaeII	RGCGCY	2 cut(s)	139, 749
HaeIII	GGCC	6 cut(s)	59, 1004, 1281, 1681, 1719, 2023
HapII	CCGG	6 cut(s)	281, 815, 924, 930, 1385, 1670
HgaI	GACGC	2 cut(s)	2092, 2092
HhaI	GCGC	5 cut(s)	26, 138, 436, 748, 2093
Hin1I	GRCGYC	1 cut(s)	2084
Hin1II	CATG	9 cut(s)	40, 64, 230, 254, 293, 589, 785, 1141, 1328
Hin6I	GCGC	5 cut(s)	24, 136, 434, 746, 2091
HinP1I	GCGC	5 cut(s)	24, 136, 434, 746, 2091
HincII	GTYRAC	4 cut(s)	664, 706, 874, 2053
HindII	GTYRAC	4 cut(s)	664, 706, 874, 2053
HindIII	AAGCTT	1 cut(s)	333
HinfI	GANTC	4 cut(s)	425, 722, 1485, 1696
HpaI	GTTAAC	1 cut(s)	706
HpaII	CCGG	6 cut(s)	281, 815, 924, 930, 1385, 1670
HphI	GGTGA	9 cut(s)	16, 658, 1038, 1043, 1203, 1383, 1398, 1524, 1659
Hpy166II	GTNNAC	7 cut(s)	664, 697, 706, 874, 1171, 1406, 2053
Hpy188I	TCNGA	7 cut(s)	424, 483, 507, 570, 1353, 2042, 2108
Hpy188III	TCNNGA	8 cut(s)	50, 313, 399, 497, 798, 1159, 1267, 1482
Hpy8I	GTNNAC	7 cut(s)	664, 697, 706, 874, 1171, 1406, 2053
Hpy99I	CGWCG	1 cut(s)	2086
HpyAV	CCTTC	6 cut(s)	103, 534, 1218, 1826, 1871, 1928
HpyCH4III	ACNGT	2 cut(s)	183, 1937
HpyCH4IV	ACGT	2 cut(s)	766, 1998
HpyF3I	CTNAG	8 cut(s)	480, 519, 537, 635, 1025, 1149, 1299, 1446
HpySE526I	ACGT	2 cut(s)	766, 1998
Hsp92I	GRCGYC	1 cut(s)	2084
Hsp92II	CATG	9 cut(s)	40, 64, 230, 254, 293, 589, 785, 1141, 1328
HspAI	GCGC	5 cut(s)	24, 136, 434, 746, 2091
KflI	GGGWCCC	1 cut(s)	1294
KroI	GCCGGC	1 cut(s)	1384
KroNI	GCCGGC	1 cut(s)	1386
Ksp22I	TGATCA	1 cut(s)	1783
KspAI	GTTAAC	1 cut(s)	706
Kzo9I	GATC	8 cut(s)	309, 419, 499, 908, 1006, 1018, 1418, 1783
LmnI	GCTCC	4 cut(s)	401, 842, 1339, 1993
LweI	GCATC	2 cut(s)	1048, 1810
MabI	ACCWGGT	4 cut(s)	1534, 1569, 1629, 1977
MaeI	CTAG	4 cut(s)	773, 869, 1641, 1766
MaeII	ACGT	2 cut(s)	766, 1998
MaeIII	GTNAC	2 cut(s)	77, 1507
MalI	GATC	8 cut(s)	311, 421, 501, 910, 1008, 1020, 1420, 1785
MboI	GATC	8 cut(s)	309, 419, 499, 908, 1006, 1018, 1418, 1783
MboII	GAAGA	4 cut(s)	313, 761, 1013, 1656
MflI	RGATCY	3 cut(s)	419, 908, 1018
MhlI	GDGCHC	1 cut(s)	847
MlyI	GAGTC	1 cut(s)	1690
MmeI	TCCRAC	2 cut(s)	165, 995
Mph1103I	ATGCAT	1 cut(s)	228
MroNI	GCCGGC	1 cut(s)	1384
MroXI	GAANNNNTTC	1 cut(s)	726
MseI	TTAA	4 cut(s)	120, 705, 945, 1107
MslI	CAYNNNNRTG	3 cut(s)	225, 730, 1691
MspA1I	CMGCKG	1 cut(s)	2096
MspI	CCGG	6 cut(s)	281, 815, 924, 930, 1385, 1670
Mva1269I	GAATGC	2 cut(s)	577, 1123
MvnI	CGCG	1 cut(s)	24
NaeI	GCCGGC	1 cut(s)	1386
NdeI	CATATG	2 cut(s)	83, 222
NdeII	GATC	8 cut(s)	309, 419, 499, 908, 1006, 1018, 1418, 1783
NgoMIV	GCCGGC	1 cut(s)	1384
NlaIII	CATG	9 cut(s)	40, 64, 230, 254, 293, 589, 785, 1141, 1328
NlaIV	GGNNCC	5 cut(s)	345, 928, 1295, 1296, 1557
NmuCI	GTSAC	1 cut(s)	77
NsiI	ATGCAT	1 cut(s)	228
NspI	RCATGY	3 cut(s)	40, 230, 1141
OliI	CACNNNNGTG	1 cut(s)	730
PasI	CCCWGGG	2 cut(s)	1437, 1552
PciI	ACATGT	1 cut(s)	1137
PcsI	WCGNNNNNNNCGW	2 cut(s)	1941, 2046
PctI	GAATGC	2 cut(s)	577, 1123
PdiI	GCCGGC	1 cut(s)	1386
PdmI	GAANNNNTTC	1 cut(s)	726
PfeI	GAWTC	3 cut(s)	425, 722, 1485
PflFI	GACNNNGTC	1 cut(s)	1040
PflMI	CCANNNNNTGG	3 cut(s)	256, 1400, 1415
PinAI	ACCGGT	1 cut(s)	1669
PleI	GAGTC	1 cut(s)	1690
PpsI	GAGTC	1 cut(s)	1690
PpuMI	RGGWCCY	2 cut(s)	1294, 1556
PscI	ACATGT	1 cut(s)	1137
PshBI	ATTAAT	1 cut(s)	1107
Psp5II	RGGWCCY	2 cut(s)	1294, 1556
PspN4I	GGNNCC	5 cut(s)	345, 928, 1295, 1296, 1557
PspPPI	RGGWCCY	2 cut(s)	1294, 1556
PstI	CTGCAG	3 cut(s)	532, 835, 1377
PstNI	CAGNNNCTG	1 cut(s)	536
PsuI	RGATCY	3 cut(s)	419, 908, 1018
PsyI	GACNNNGTC	1 cut(s)	1040
PvuII	CAGCTG	1 cut(s)	2096
RsaI	GTAC	2 cut(s)	696, 1934
RsaNI	GTAC	2 cut(s)	695, 1933
RseI	CAYNNNNRTG	3 cut(s)	225, 730, 1691
SaqAI	TTAA	4 cut(s)	120, 705, 945, 1107
Sau3AI	GATC	8 cut(s)	309, 419, 499, 908, 1006, 1018, 1418, 1783
SchI	GAGTC	1 cut(s)	1690
SduI	GDGCHC	1 cut(s)	847
SexAI	ACCWGGT	4 cut(s)	1534, 1569, 1629, 1977
SfaNI	GCATC	2 cut(s)	1048, 1810
SfcI	CTRYAG	3 cut(s)	528, 831, 1373
SinI	GGWCC	7 cut(s)	47, 344, 863, 1294, 1556, 1637, 1672
SmiMI	CAYNNNNRTG	3 cut(s)	225, 730, 1691
SmlI	CTYRAG	3 cut(s)	550, 916, 1889
SmoI	CTYRAG	3 cut(s)	550, 916, 1889
SpeI	ACTAGT	1 cut(s)	1765
SsiI	CCGC	4 cut(s)	716, 912, 1029, 1217
SspMI	CTAG	4 cut(s)	773, 869, 1641, 1766
StyI	CCWWGG	3 cut(s)	139, 1515, 1675
TaaI	ACNGT	2 cut(s)	183, 1937
TaiI	ACGT	2 cut(s)	769, 2001
TaqI	TCGA	4 cut(s)	69, 710, 1134, 1483
TaqII	GACCGA	2 cut(s)	35, 361
TfiI	GAWTC	3 cut(s)	425, 722, 1485
Tru1I	TTAA	4 cut(s)	120, 705, 945, 1107
Tru9I	TTAA	4 cut(s)	120, 705, 945, 1107
TscAI	CASTG	7 cut(s)	186, 674, 883, 1153, 1377, 1420, 1506
TseFI	GTSAC	1 cut(s)	77
Tsp45I	GTSAC	1 cut(s)	77
TspDTI	ATGAA	7 cut(s)	77, 209, 735, 798, 1053, 1979, 2081
TspGWI	ACGGA	4 cut(s)	575, 855, 1287, 1933
TspRI	CASTG	7 cut(s)	186, 674, 883, 1153, 1377, 1420, 1506
Tth111I	GACNNNGTC	1 cut(s)	1040
Van91I	CCANNNNNTGG	3 cut(s)	256, 1400, 1415
VpaK11BI	GGWCC	7 cut(s)	47, 344, 863, 1294, 1556, 1637, 1672
VspI	ATTAAT	1 cut(s)	1107
XapI	RAATTY	4 cut(s)	151, 484, 564, 1237
XceI	RCATGY	3 cut(s)	40, 230, 1141
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1434
XmnI	GAANNNNTTC	1 cut(s)	726
XspI	CTAG	4 cut(s)	773, 869, 1641, 1766
Zsp2I	ATGCAT	1 cut(s)	228

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.