Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011660

Physical Location & Seq

Genomic Coordinates Reverse (-)

7401220 .. 7404520

Gene Structure 3301 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1106000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGCATATTGATGTTATCAAACTTACAAGCATGGAGTTGAAGAAAGACAAGGTATTAAGGTTTTATGATCATGATAAGAAAGATGTTCAGTTTTCCTGGGAAAAAAGTGATATCCAAAAACACTCAGAAGAGCCACTATCTGCATACAAAATATCAACAGCAGCACCCTTGTTAATTCCTGATGGTGGTACTAGTACTACTACCGGAGTTCGAACTAATAAAACTCCGAGATTTGGGAGTTTTAAGGTTTTCTCAGAGAACCAGGAGCCACGTAAGAAGCAAATTCTTGATCCGGGAAGTGACATTTTCCTGCAATGGAACAGGGTTTTTCTCTTTTCTTGCTTGGTAGCCTTGTTCGTTGATCCGCTTTTTTTCTACCTCCCTTCGGTGCAAAATGACGGTAGTTCATCTTGTATGACAACTGATTTGAATTTAGGAATTGTTGTGACGTTTTTTCGCACTCTCGCGGACATGTTCTATCTGTTGCACATGTTCATTAAGTTAAGGACAGCTTATGTATCCCCGAGTTCGAGAATTTTCGGGAGGGGGGAACTTGTTATGGATCCAAAGAAGATTGCTCGAAGGTATTTGAGAACGGATTTCTTCATAGATCTCATAGCTACACTGCCTCTCCCTCAGATTGTGATATGGTTTATTATACCTGCAGTTAGAAGCTCACATGCTACCAATAATGCCCTTGTACTGATTGTACTTCTTCAGTATATTCCTAGACTCTATCTTATTTTCCCATTAAGCTCTCAGATCATCAAAGCCACTGGTGTAGTCACAAAGACCGCCTGGGCGGGGGCTGCGTATAATCTCGCACTCTACATGTTAGCCAGTCATATCTTAGGGGCATCGTGGTACTTGTTGTCCATTGAACGGTATGCAACGTGCTGGAAGTCTGAATGCCGAAGGGAATTCAGCCCCATGAAGTGTTTTCTCAGCTACTTGGATTGTGGTAATTTGAACAATGATAACCGCCGGACGTGGTTGAACAGTACTCTAGTGTTTAGTAACTGCAATCCGGAAACCAGCACCAGTTTCAACTATGGCATATTTGCAAATGCACTGACGAAAAATGTCGTCTCCACGGGGTTTCTTGAGAAGTACTTCTACTGTCTTTGGTGGGGTTTGCAGAACTTGAGCTCCTATGGTCAGAATTTGAGTACAAGCACATTTATCGGGGAAACGTCATTTGCAATACTAATTGCAATCTTGGGTTTGGTTCTGTTTGCTCACTTAATCGGAAATATGCAGACCTATTTGCAATCGATCACGTTGAGACTTGAGGAGTGGAGGCTCAAGCGGCGAGACACAGAGGAGTGGATGAGACACCGTCAACTCCCCCAAGATCTGCAAGAGCGCGTTAGGCGATTCGTGCAGTACAAGTGGGTTGCCACTCGAGGGGTTGATGAAGAATCAATCTTACATGCCCTGCCTACTGATCTTCGTCGAGATATCCAACGCCACTTGTGTTTAGATCTTGTTCGACGCGTCCCATTTTTCGCACAGATGGACGGACAACTACTTGACGCCATATGCGAGCGTTTGGTATCCTCATTAAGTACTCAGGGCACGTACATTGTTCGCGAAGGCGACCCTGTCACAGAGATGCTTTTCGTTATCCGAGGGAAACTAGAGAGCTCCACAACAAATGGAGGCAGGACAGGTTTCTTCAACTCAATCACTTTGAGGCCCGGCGATTTTTGTGGAGAAGAGTTGCTTGCTTGGGCATTGCTTCCCAAATCAACCCTCAACTTGCCTTCTTCCACAAGAACTGTTAAATCCCTCGACGAAGTAGAAGCCTTTGCGCTGAGAGCAGAAGACCTCAAATTCGTCGCGAATCAGTTTAGGCGCCTCCACAGCAAGAAGTTACAGCACACGTTTCGTTTCTACTCCTACCATTGGAGGACCTGGGCTGCATGCTTCATTCAGGTAGCGTGGCGGCGGTTCAGGAAGAGAATGTTGGCGAAGAACCTTAGCATGTCAGAGTCATTCTCGTACAATTACGACAAACACGACGAAGAGGCTGATGATACTGAAGAAGAAAAGGAAGAGTTTTCTAGTGGTAAAGAAACTTCGAATACTTTCCAAGTGAGACAGAATCTTGGTGTCACAATTTTGGCTTCAAGGTTTGCTGCAAACACTAGGAGAGGAGCTCAAAAAACCAAGGATGTTCAGCTGCCGAAGTTGCTAAAACCTGAAGAGCCAGACTTCTCAATGGAGCCAGAAGATGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

748

Amino Acids

86.2

Weight (kDa)

8.96

Isoelectric Point (pI)

46.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	106 - 429	6.9e-19	Ion transport protein
cNMP_binding	PF00027	525 - 614	2.1e-07	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011660)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g36620
malus_domestica	MD15G1106000.v1.1
prunus_persica	Prupe.1G457900_v2.0.a1
pyrus_communis	pycom15g09630 pycom15g09650
rosa_chinensis	RchiOBHm_Chr6g0305181
rosa_laevigata	RLG00000010910
rosa_multiflora	Rmu_sc0000061.1_g000007 Rmu_sc0000061.1_g000010
rosa_roxburghii	Rroxscaffold_7G00163060 Rroxscaffold_7G00163070
rosa_rugosa	Rorug06G0338300
rosa_samantha	Rh6AG450000 Rh6BG451400 Rh6CG463700 Rh6CG463800 Rh6DG450600
rosa_wichuraiana	Rw6G039200 Rw6G039220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	670
AccB1I	GGYRCC	1 cut(s)	1857
AccII	CGCG	5 cut(s)	467, 1368, 1497, 1593, 1844
AccIII	TCCGGA	1 cut(s)	1027
AciI	CCGC	8 cut(s)	365, 467, 795, 803, 982, 1309, 1948, 1951
AclWI	GGATC	4 cut(s)	284, 356, 557, 570
AcsI	RAATTY	6 cut(s)	282, 430, 534, 920, 1162, 1835
AcuI	CTGAAG	3 cut(s)	701, 2064, 2226
AcyI	GRCGYC	2 cut(s)	1536, 1858
AfiI	CCNNNNNNNGG	7 cut(s)	185, 233, 385, 804, 882, 1407, 1908
AflIII	ACRYGT	5 cut(s)	471, 489, 831, 1495, 1884
AgsI	TTSAA	8 cut(s)	40, 430, 881, 970, 997, 1048, 1681, 2133
AhlI	ACTAGT	1 cut(s)	191
AjiI	CACGTC	1 cut(s)	990
AjnI	CCWGG	4 cut(s)	95, 261, 797, 1916
AjuI	GAANNNNNNNTTGG	2 cut(s)	1952, 1984
AloI	GAACNNNNNNTCC	2 cut(s)	1133, 1165
AluBI	AGCT	9 cut(s)	512, 620, 675, 756, 948, 1149, 1647, 2162, 2185
AluI	AGCT	9 cut(s)	512, 620, 675, 756, 948, 1149, 1647, 2162, 2185
Alw21I	GWGCWC	3 cut(s)	1151, 1649, 2164
Alw26I	GTCTC	5 cut(s)	1093, 1279, 1308, 1327, 2095
AlwI	GGATC	4 cut(s)	284, 356, 557, 570
Ama87I	CYCGRG	2 cut(s)	523, 1404
Aor13HI	TCCGGA	1 cut(s)	1027
AoxI	GGCC	1 cut(s)	1697
ApeKI	GCWGC	5 cut(s)	161, 809, 1922, 2141, 2185
ApoI	RAATTY	6 cut(s)	282, 430, 534, 920, 1162, 1835
ArsI	GACNNNNNNTTYG	4 cut(s)	409, 441, 1820, 1852
Asp700I	GAANNNNTTC	2 cut(s)	1112, 2090
AspLEI	GCGC	3 cut(s)	1368, 1816, 1860
AspS9I	GGNCC	2 cut(s)	1698, 1914
AsuC2I	CCSGG	2 cut(s)	294, 1701
AsuII	TTCGAA	2 cut(s)	211, 2084
AvaI	CYCGRG	2 cut(s)	523, 1404
AvaII	GGWCC	1 cut(s)	1914
BaeGI	GKGCMC	1 cut(s)	1580
BamHI	GGATCC	1 cut(s)	562
BanI	GGYRCC	1 cut(s)	1857
BanII	GRGCYC	3 cut(s)	1151, 1649, 2164
BarI	GAAGNNNNNNTAC	4 cut(s)	1100, 1132, 2065, 2097
BbsI	GAAGAC	1 cut(s)	1833
Bbv12I	GWGCWC	3 cut(s)	1151, 1649, 2164
BbvI	GCAGC	5 cut(s)	173, 796, 1909, 2128, 2172
BccI	CCATC	2 cut(s)	176, 1510
BcgI	CGANNNNNNTGC	4 cut(s)	1165, 1199, 1871, 1905
BciT130I	CCWGG	4 cut(s)	97, 263, 799, 1918
BciVI	GTATCC	2 cut(s)	529, 1567
BclI	TGATCA	1 cut(s)	67
BcnI	CCSGG	2 cut(s)	294, 1701
BcoDI	GTCTC	5 cut(s)	1093, 1279, 1308, 1327, 2095
BcuI	ACTAGT	1 cut(s)	191
BfaI	CTAG	7 cut(s)	192, 729, 1007, 1640, 2067, 2151, 2242
BfmI	CTRYAG	1 cut(s)	663
BfoI	RGCGCY	1 cut(s)	1861
BfuAI	ACCTGC	1 cut(s)	670
BfuI	GTATCC	2 cut(s)	529, 1567
BglII	AGATCT	3 cut(s)	610, 1354, 1483
BisI	GCNGC	7 cut(s)	162, 810, 1310, 1923, 1949, 2142, 2186
BlsI	GCNGC	7 cut(s)	163, 811, 1311, 1924, 1950, 2143, 2187
BmcAI	AGTACT	4 cut(s)	196, 1003, 1112, 1570
Bme1390I	CCNGG	6 cut(s)	97, 263, 294, 799, 1701, 1918
Bme18I	GGWCC	1 cut(s)	1914
BmeT110I	CYCGRG	2 cut(s)	523, 1404
BmgBI	CACGTC	1 cut(s)	990
BmgT120I	GGNCC	2 cut(s)	1698, 1914
BmiI	GGNNCC	4 cut(s)	267, 564, 1859, 2229
BmrFI	CCNGG	6 cut(s)	97, 263, 294, 799, 1701, 1918
BmsI	GCATC	2 cut(s)	866, 1605
BpiI	GAAGAC	1 cut(s)	1833
BplI	GAGNNNNNCTC	4 cut(s)	1985, 2017, 2146, 2178
Bpu10I	CCTNAGC	1 cut(s)	1982
Bpu14I	TTCGAA	2 cut(s)	211, 2084
BpuEI	CTTGAG	4 cut(s)	1124, 1165, 1289, 1310
BpuMI	CCSGG	2 cut(s)	294, 1701
Bsa29I	ATCGAT	1 cut(s)	1274
BsaAI	YACGTR	2 cut(s)	272, 1581
BsaHI	GRCGYC	2 cut(s)	1536, 1858
BsaJI	CCNNGG	6 cut(s)	96, 798, 1092, 1630, 1917, 2172
BsaWI	WCCGGW	2 cut(s)	203, 1027
BsaXI	ACNNNNNCTCC	4 cut(s)	615, 645, 1133, 1163
Bsc4I	CCNNNNNNNGG	7 cut(s)	185, 233, 385, 804, 882, 1407, 1908
Bse1I	ACTGG	3 cut(s)	781, 840, 1041
Bse3DI	GCAATG	2 cut(s)	320, 1736
BseAI	TCCGGA	1 cut(s)	1027
BseBI	CCWGG	4 cut(s)	97, 263, 799, 1918
BseCI	ATCGAT	1 cut(s)	1274
BseDI	CCNNGG	6 cut(s)	96, 798, 1092, 1630, 1917, 2172
BseGI	GGATG	2 cut(s)	1335, 2182
BseLI	CCNNNNNNNGG	7 cut(s)	185, 233, 385, 804, 882, 1407, 1908
BseMI	GCAATG	2 cut(s)	320, 1736
BseMII	CTCAG	7 cut(s)	138, 267, 650, 773, 958, 1586, 1808
BseNI	ACTGG	3 cut(s)	781, 840, 1041
BseRI	GAGGAG	3 cut(s)	1307, 1337, 2172
BseSI	GKGCMC	1 cut(s)	1580
BseXI	GCAGC	5 cut(s)	173, 796, 1909, 2128, 2172
BsgI	GTGCAG	1 cut(s)	1403
Bsh1236I	CGCG	5 cut(s)	467, 1368, 1497, 1593, 1844
BshFI	GGCC	1 cut(s)	1699
BshNI	GGYRCC	1 cut(s)	1857
BshVI	ATCGAT	1 cut(s)	1274
BsiHKAI	GWGCWC	3 cut(s)	1151, 1649, 2164
BsiHKCI	CYCGRG	2 cut(s)	523, 1404
BsiSI	CCGG	5 cut(s)	204, 293, 985, 1028, 1701
BslFI	GGGAC	1 cut(s)	1484
BslI	CCNNNNNNNGG	7 cut(s)	185, 233, 385, 804, 882, 1407, 1908
BsmAI	GTCTC	5 cut(s)	1093, 1279, 1308, 1327, 2095
BsmBI	CGTCTC	1 cut(s)	1093
BsmFI	GGGAC	1 cut(s)	1484
BsmI	GAATGC	1 cut(s)	914
BsnI	GGCC	1 cut(s)	1699
BsoBI	CYCGRG	2 cut(s)	523, 1404
Bsp119I	TTCGAA	2 cut(s)	211, 2084
Bsp1286I	GDGCHC	4 cut(s)	1151, 1580, 1649, 2164
Bsp13I	TCCGGA	1 cut(s)	1027
Bsp68I	TCGCGA	2 cut(s)	1593, 1844
BspACI	CCGC	8 cut(s)	365, 467, 795, 803, 982, 1309, 1948, 1951
BspANI	GGCC	1 cut(s)	1699
BspCNI	CTCAG	7 cut(s)	137, 266, 649, 772, 957, 1585, 1809
BspDI	ATCGAT	1 cut(s)	1274
BspEI	TCCGGA	1 cut(s)	1027
BspFNI	CGCG	5 cut(s)	467, 1368, 1497, 1593, 1844
BspHI	TCATGA	1 cut(s)	70
BspLI	GGNNCC	4 cut(s)	267, 564, 1859, 2229
BspMAI	CTGCAG	1 cut(s)	667
BspMI	ACCTGC	1 cut(s)	670
BspPI	GGATC	4 cut(s)	284, 356, 557, 570
BspQI	GCTCTTC	2 cut(s)	123, 2202
BspT104I	TTCGAA	2 cut(s)	211, 2084
BspT107I	GGYRCC	1 cut(s)	1857
BsrDI	GCAATG	2 cut(s)	320, 1736
BsrI	ACTGG	3 cut(s)	781, 840, 1041
BssECI	CCNNGG	6 cut(s)	96, 798, 1092, 1630, 1917, 2172
BssNI	GRCGYC	2 cut(s)	1536, 1858
BssT1I	CCWWGG	1 cut(s)	2172
Bst2UI	CCWGG	4 cut(s)	97, 263, 799, 1918
Bst4CI	ACNGT	6 cut(s)	401, 885, 1001, 1121, 1340, 1783
Bst6I	CTCTTC	6 cut(s)	123, 1713, 1955, 2022, 2052, 2202
BstACI	GRCGYC	2 cut(s)	1536, 1858
BstBAI	YACGTR	2 cut(s)	272, 1581
BstBI	TTCGAA	2 cut(s)	211, 2084
BstC8I	GCNNGC	3 cut(s)	1547, 1728, 1927
BstDEI	CTNAG	9 cut(s)	124, 253, 636, 759, 850, 944, 1572, 1817, 1982
BstDSI	CCRYGG	1 cut(s)	1092
BstF5I	GGATG	2 cut(s)	1335, 2182
BstFNI	CGCG	5 cut(s)	467, 1368, 1497, 1593, 1844
BstH2I	RGCGCY	1 cut(s)	1861
BstHHI	GCGC	3 cut(s)	1368, 1816, 1860
BstMAI	GTCTC	5 cut(s)	1093, 1279, 1308, 1327, 2095
BstMWI	GCNNNNNNNGC	7 cut(s)	809, 1309, 1372, 1381, 1820, 1866, 2194
BstNI	CCWGG	4 cut(s)	97, 263, 799, 1918
BstNSI	RCATGY	7 cut(s)	475, 493, 683, 835, 1436, 1929, 1990
BstSCI	CCNGG	6 cut(s)	95, 261, 292, 797, 1699, 1916
BstSFI	CTRYAG	1 cut(s)	663
BstSLI	GKGCMC	1 cut(s)	1580
BstUI	CGCG	5 cut(s)	467, 1368, 1497, 1593, 1844
BstV1I	GCAGC	5 cut(s)	173, 796, 1909, 2128, 2172
BstV2I	GAAGAC	1 cut(s)	1833
BstX2I	RGATCY	4 cut(s)	562, 610, 1354, 1483
BstYI	RGATCY	4 cut(s)	562, 610, 1354, 1483
Bsu15I	ATCGAT	1 cut(s)	1274
BsuI	GTATCC	2 cut(s)	529, 1567
BsuRI	GGCC	1 cut(s)	1699
BsuTUI	ATCGAT	1 cut(s)	1274
BtgI	CCRYGG	1 cut(s)	1092
BtrI	CACGTC	1 cut(s)	990
BtsCI	GGATG	2 cut(s)	1335, 2182
BtsI	GCAGTG	1 cut(s)	623
BtsIMutI	CAGTG	3 cut(s)	623, 774, 1070
BtuMI	TCGCGA	2 cut(s)	1593, 1844
BveI	ACCTGC	1 cut(s)	670
Cac8I	GCNNGC	3 cut(s)	1547, 1728, 1927
CciI	TCATGA	1 cut(s)	70
CfoI	GCGC	3 cut(s)	1368, 1816, 1860
Cfr13I	GGNCC	2 cut(s)	1698, 1914
ClaI	ATCGAT	1 cut(s)	1274
CseI	GACGC	3 cut(s)	1486, 1503, 1544
DdeI	CTNAG	9 cut(s)	124, 253, 636, 759, 850, 944, 1572, 1817, 1982
DinI	GGCGCC	1 cut(s)	1859
Eam1104I	CTCTTC	6 cut(s)	123, 1713, 1955, 2022, 2052, 2202
EarI	CTCTTC	6 cut(s)	123, 1713, 1955, 2022, 2052, 2202
Ecl136II	GAGCTC	3 cut(s)	1149, 1647, 2162
Eco130I	CCWWGG	1 cut(s)	2172
Eco24I	GRGCYC	3 cut(s)	1151, 1649, 2164
Eco32I	GATATC	2 cut(s)	112, 1462
Eco47I	GGWCC	1 cut(s)	1914
Eco53kI	GAGCTC	3 cut(s)	1149, 1647, 2162
Eco57I	CTGAAG	3 cut(s)	701, 2064, 2226
Eco88I	CYCGRG	2 cut(s)	523, 1404
EcoICRI	GAGCTC	3 cut(s)	1149, 1647, 2162
EcoO109I	RGGNCCY	1 cut(s)	1914
EcoRI	GAATTC	1 cut(s)	920
EcoRII	CCWGG	4 cut(s)	95, 261, 797, 1916
EcoRV	GATATC	2 cut(s)	112, 1462
EcoT14I	CCWWGG	1 cut(s)	2172
EcoT22I	ATGCAT	1 cut(s)	6
EcoT38I	GRGCYC	3 cut(s)	1151, 1649, 2164
EgeI	GGCGCC	1 cut(s)	1859
EheI	GGCGCC	1 cut(s)	1859
ErhI	CCWWGG	1 cut(s)	2172
Esp3I	CGTCTC	1 cut(s)	1093
FalI	AAGNNNNNCTT	4 cut(s)	496, 528, 1710, 1742
FaqI	GGGAC	1 cut(s)	1484
FauI	CCCGC	1 cut(s)	796
FauNDI	CATATG	1 cut(s)	1541
FbaI	TGATCA	1 cut(s)	67
Fnu4HI	GCNGC	7 cut(s)	162, 810, 1310, 1923, 1949, 2142, 2186
FokI	GGATG	2 cut(s)	1342, 2189
FriOI	GRGCYC	3 cut(s)	1151, 1649, 2164
Fsp4HI	GCNGC	7 cut(s)	162, 810, 1310, 1923, 1949, 2142, 2186
FspBI	CTAG	7 cut(s)	192, 729, 1007, 1640, 2067, 2151, 2242
GlaI	GCGC	3 cut(s)	1367, 1815, 1859
GluI	GCNGC	7 cut(s)	162, 810, 1310, 1923, 1949, 2142, 2186
HaeII	RGCGCY	1 cut(s)	1861
HaeIII	GGCC	1 cut(s)	1699
HapII	CCGG	5 cut(s)	204, 293, 985, 1028, 1701
HgaI	GACGC	3 cut(s)	1486, 1503, 1544
HhaI	GCGC	3 cut(s)	1368, 1816, 1860
Hin1I	GRCGYC	2 cut(s)	1536, 1858
Hin6I	GCGC	3 cut(s)	1366, 1814, 1858
HinP1I	GCGC	3 cut(s)	1366, 1814, 1858
HincII	GTYRAC	1 cut(s)	1343
HindII	GTYRAC	1 cut(s)	1343
HinfI	GANTC	6 cut(s)	732, 1377, 1421, 1846, 1994, 2107
HpaII	CCGG	5 cut(s)	204, 293, 985, 1028, 1701
Hpy166II	GTNNAC	1 cut(s)	1343
Hpy8I	GTNNAC	1 cut(s)	1343
Hpy99I	CGWCG	5 cut(s)	1458, 1497, 1799, 1844, 2027
HpyAV	CCTTC	5 cut(s)	393, 576, 909, 1589, 1776
HpyCH4III	ACNGT	6 cut(s)	401, 885, 1001, 1121, 1340, 1783
HpyCH4IV	ACGT	8 cut(s)	271, 449, 893, 989, 1193, 1280, 1580, 1886
HpyF10VI	GCNNNNNNNGC	7 cut(s)	809, 1309, 1372, 1381, 1820, 1866, 2194
HpyF3I	CTNAG	9 cut(s)	124, 253, 636, 759, 850, 944, 1572, 1817, 1982
HpySE526I	ACGT	8 cut(s)	271, 449, 893, 989, 1193, 1280, 1580, 1886
Hsp92I	GRCGYC	2 cut(s)	1536, 1858
HspAI	GCGC	3 cut(s)	1366, 1814, 1858
KasI	GGCGCC	1 cut(s)	1857
Kpn2I	TCCGGA	1 cut(s)	1027
Ksp22I	TGATCA	1 cut(s)	67
LguI	GCTCTTC	2 cut(s)	123, 2202
LmnI	GCTCC	5 cut(s)	265, 1154, 1652, 2159, 2227
Lsp1109I	GCAGC	5 cut(s)	173, 796, 1909, 2128, 2172
LweI	GCATC	2 cut(s)	866, 1605
MaeI	CTAG	7 cut(s)	192, 729, 1007, 1640, 2067, 2151, 2242
MaeII	ACGT	8 cut(s)	271, 449, 893, 989, 1193, 1280, 1580, 1886
MaeIII	GTNAC	7 cut(s)	299, 445, 784, 1016, 1606, 1875, 2116
MflI	RGATCY	4 cut(s)	562, 610, 1354, 1483
MhlI	GDGCHC	4 cut(s)	1151, 1580, 1649, 2164
MluI	ACGCGT	1 cut(s)	1495
Mly113I	GGCGCC	1 cut(s)	1858
MlyI	GAGTC	2 cut(s)	726, 2003
MmeI	TCCRAC	1 cut(s)	1489
Mph1103I	ATGCAT	1 cut(s)	6
MroI	TCCGGA	1 cut(s)	1027
MroXI	GAANNNNTTC	2 cut(s)	1112, 2090
MseI	TTAA	9 cut(s)	56, 173, 243, 498, 503, 752, 1244, 1565, 1785
MslI	CAYNNNNRTG	2 cut(s)	9, 1613
MspA1I	CMGCKG	1 cut(s)	2185
MspI	CCGG	5 cut(s)	204, 293, 985, 1028, 1701
MspR9I	CCNGG	6 cut(s)	97, 263, 294, 799, 1701, 1918
Mva1269I	GAATGC	1 cut(s)	914
MvaI	CCWGG	4 cut(s)	97, 263, 799, 1918
MvnI	CGCG	5 cut(s)	467, 1368, 1497, 1593, 1844
MwoI	GCNNNNNNNGC	7 cut(s)	809, 1309, 1372, 1381, 1820, 1866, 2194
NarI	GGCGCC	1 cut(s)	1858
NciI	CCSGG	2 cut(s)	294, 1701
NdeI	CATATG	1 cut(s)	1541
NlaIV	GGNNCC	4 cut(s)	267, 564, 1859, 2229
NmuCI	GTSAC	5 cut(s)	299, 445, 784, 1606, 2116
NruI	TCGCGA	2 cut(s)	1593, 1844
NsiI	ATGCAT	1 cut(s)	6
NspI	RCATGY	7 cut(s)	475, 493, 683, 835, 1436, 1929, 1990
NspV	TTCGAA	2 cut(s)	211, 2084
PaeI	GCATGC	1 cut(s)	1929
PaeR7I	CTCGAG	1 cut(s)	1404
PagI	TCATGA	1 cut(s)	70
PciI	ACATGT	3 cut(s)	471, 489, 831
PciSI	GCTCTTC	2 cut(s)	123, 2202
PcsI	WCGNNNNNNNCGW	2 cut(s)	1542, 2019
PctI	GAATGC	1 cut(s)	914
PdmI	GAANNNNTTC	2 cut(s)	1112, 2090
PfeI	GAWTC	4 cut(s)	1377, 1421, 1846, 2107
PflFI	GACNNNGTC	2 cut(s)	1338, 1604
PfoI	TCCNGGA	1 cut(s)	292
PkrI	GCNGC	7 cut(s)	163, 811, 1311, 1924, 1950, 2143, 2187
PleI	GAGTC	2 cut(s)	726, 2002
PluTI	GGCGCC	1 cut(s)	1861
PpsI	GAGTC	2 cut(s)	726, 2002
Ppu21I	YACGTR	2 cut(s)	272, 1581
PpuMI	RGGWCCY	1 cut(s)	1914
PscI	ACATGT	3 cut(s)	471, 489, 831
Psp124BI	GAGCTC	3 cut(s)	1151, 1649, 2164
Psp5II	RGGWCCY	1 cut(s)	1914
Psp6I	CCWGG	4 cut(s)	95, 261, 797, 1916
PspGI	CCWGG	4 cut(s)	95, 261, 797, 1916
PspN4I	GGNNCC	4 cut(s)	267, 564, 1859, 2229
PspPI	GGNCC	2 cut(s)	1698, 1914
PspPPI	RGGWCCY	1 cut(s)	1914
PspXI	VCTCGAGB	1 cut(s)	1404
PstI	CTGCAG	1 cut(s)	667
PsuI	RGATCY	4 cut(s)	562, 610, 1354, 1483
PsyI	GACNNNGTC	2 cut(s)	1338, 1604
PvuII	CAGCTG	1 cut(s)	2185
RruI	TCGCGA	2 cut(s)	1593, 1844
RseI	CAYNNNNRTG	2 cut(s)	9, 1613
SacI	GAGCTC	3 cut(s)	1151, 1649, 2164
SapI	GCTCTTC	2 cut(s)	123, 2202
SaqAI	TTAA	9 cut(s)	56, 173, 243, 498, 503, 752, 1244, 1565, 1785
SatI	GCNGC	7 cut(s)	162, 810, 1310, 1923, 1949, 2142, 2186
Sau96I	GGNCC	2 cut(s)	1698, 1914
ScaI	AGTACT	4 cut(s)	196, 1003, 1112, 1570
SchI	GAGTC	2 cut(s)	726, 2003
ScrFI	CCNGG	6 cut(s)	97, 263, 294, 799, 1701, 1918
SduI	GDGCHC	4 cut(s)	1151, 1580, 1649, 2164
SfaNI	GCATC	2 cut(s)	866, 1605
SfcI	CTRYAG	1 cut(s)	663
SfoI	GGCGCC	1 cut(s)	1859
Sfr274I	CTCGAG	1 cut(s)	1404
SfuI	TTCGAA	2 cut(s)	211, 2084
SinI	GGWCC	1 cut(s)	1914
SlaI	CTCGAG	1 cut(s)	1404
SmiMI	CAYNNNNRTG	2 cut(s)	9, 1613
SmlI	CTYRAG	5 cut(s)	1103, 1144, 1289, 1304, 1404
SmoI	CTYRAG	5 cut(s)	1103, 1144, 1289, 1304, 1404
SpeI	ACTAGT	1 cut(s)	191
SphI	GCATGC	1 cut(s)	1929
SsiI	CCGC	8 cut(s)	365, 467, 795, 803, 982, 1309, 1948, 1951
SspDI	GGCGCC	1 cut(s)	1857
SspMI	CTAG	7 cut(s)	192, 729, 1007, 1640, 2067, 2151, 2242
SstI	GAGCTC	3 cut(s)	1151, 1649, 2164
StyD4I	CCNGG	6 cut(s)	95, 261, 292, 797, 1699, 1916
StyI	CCWWGG	1 cut(s)	2172
TaaI	ACNGT	6 cut(s)	401, 885, 1001, 1121, 1340, 1783
TaiI	ACGT	8 cut(s)	274, 452, 896, 992, 1196, 1283, 1583, 1889
TaqI	TCGA	9 cut(s)	211, 530, 580, 1274, 1405, 1456, 1492, 1794, 2084
TatI	WGTACW	8 cut(s)	194, 700, 709, 1001, 1110, 1169, 1386, 1568
TauI	GCSGC	2 cut(s)	1312, 1951
TfiI	GAWTC	4 cut(s)	1377, 1421, 1846, 2107
Tru1I	TTAA	9 cut(s)	56, 173, 243, 498, 503, 752, 1244, 1565, 1785
Tru9I	TTAA	9 cut(s)	56, 173, 243, 498, 503, 752, 1244, 1565, 1785
TscAI	CASTG	3 cut(s)	630, 781, 1077
TseFI	GTSAC	5 cut(s)	299, 445, 784, 1606, 2116
TseI	GCWGC	5 cut(s)	161, 809, 1922, 2141, 2185
Tsp45I	GTSAC	5 cut(s)	299, 445, 784, 1606, 2116
TspDTI	ATGAA	6 cut(s)	396, 484, 595, 947, 1431, 1921
TspGWI	ACGGA	2 cut(s)	611, 1536
TspRI	CASTG	3 cut(s)	630, 781, 1077
Tth111I	GACNNNGTC	2 cut(s)	1338, 1604
VpaK11BI	GGWCC	1 cut(s)	1914
XapI	RAATTY	6 cut(s)	282, 430, 534, 920, 1162, 1835
XceI	RCATGY	7 cut(s)	475, 493, 683, 835, 1436, 1929, 1990
XhoI	CTCGAG	1 cut(s)	1404
XmnI	GAANNNNTTC	2 cut(s)	1112, 2090
XspI	CTAG	7 cut(s)	192, 729, 1007, 1640, 2067, 2151, 2242
ZrmI	AGTACT	4 cut(s)	196, 1003, 1112, 1570
Zsp2I	ATGCAT	1 cut(s)	6

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.