Rroxscaffold_7G00163060

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0011660
Physical Location & Seq
Reverse (-)
5397889 .. 5400125
2237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00163060.1

Sequence Viewer

Length: 915 bp
ATGAGACATAGGCAACTCCCTCAAGATCTGCAAGAACGTGTTAGGCGATTTGTGCAGTACAAGTGGCTTGCAACTCGAGGAGTTGATGAAGAATCAATCTTACACGCCTTTCCTACAGATCTTCGTCGAGAAATCAAACGTCACCTATGTTTAGATCTTGTTCGACGCGTCCCATTTTTCTCACAGATAGATGGTCAACTACTTGATGCCATATGCGAGCGTTTGGTATCTTCCTTGAGTACTCAAGGCACGTACATTGTTCGAGAAGGTGACCCTGTCACAGAGATGTTACTCATTATCCGAGGAAAGCTAGAGAGCTCAACAACAAAAGGAGGTCGGACAGGTTTCTTCAACTCAAATACTTTGAGACCTGGTGATTTTTGTGAGGAAGAATTGCTTGCTTGGGTATTGCTTCCCAACTCTACTCTCAACTTGCCTTCTTCTACAAGAACTGTTAGATCACTTAATGAAGTAGAGGCCTTTGCACTTAGAGCAGAAGATCTCAAGTTCGTTGCTAATCAGTTTCGACGACTTCATGGCAAGAAGTTGCAACACACATTCCGATTCTACTCTTACCACTGGAGAACTTGGGCTGCATGCTTCATTCAGGTTGCGTGGCGTCAATTCAAGAAGAGATTGTTGGCAGAGAATCTTAGCATGGTTGAGTCATTTTCATATAACTGTGACAACCAAGAGGCTGATGAGGCCGAACAAGAAAAGGAGGAGTACTTTAGGACTGATGCACCTTCAAATGCTTCGCAAGCGAGACAGAATTTGGGTTTAGGTGACTGCCAAAGCCGAGAAGAGGGCAACTGCCAAAGCCTGAAGAGCCAGACTTCTCAGTATAGCCAGAAGATGACTAGTAACTACTTCATTACTATGTACAGATTCATAAATTATGTTCAACGCCTATAG

Protein Analysis

304

Amino Acids

35.64

Weight (kDa)

8.8

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 82 - 171 7.2e-07 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 168
AcsI RAATTY 1 cut(s) 772
AcuI CTGAAG 1 cut(s) 845
AcyI GRCGYC 1 cut(s) 619
AfaI GTAC 5 cut(s) 59, 241, 254, 728, 884
AfiI CCNNNNNNNGG 1 cut(s) 805
AflIII ACRYGT 2 cut(s) 37, 166
AgsI TTSAA 4 cut(s) 352, 628, 750, 905
AhlI ACTAGT 1 cut(s) 860
AjnI CCWGG 1 cut(s) 370
AjuI GAANNNNNNNTTGG 2 cut(s) 623, 655
AluBI AGCT 2 cut(s) 310, 318
AluI AGCT 2 cut(s) 310, 318
Alw21I GWGCWC 1 cut(s) 320
Alw26I GTCTC 2 cut(s) 361, 760
Ama87I CYCGRG 1 cut(s) 75
AoxI GGCC 2 cut(s) 477, 705
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 1 cut(s) 772
AsuHPI GGTGA 4 cut(s) 134, 281, 386, 797
AvaI CYCGRG 1 cut(s) 75
BanII GRGCYC 1 cut(s) 320
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 580
BccI CCATC 1 cut(s) 185
BciT130I CCWGG 1 cut(s) 372
BcoDI GTCTC 2 cut(s) 361, 760
BcuI ACTAGT 1 cut(s) 860
BfaI CTAG 2 cut(s) 311, 861
BfmI CTRYAG 2 cut(s) 114, 911
BglII AGATCT 4 cut(s) 25, 118, 154, 499
BisI GCNGC 1 cut(s) 594
BlsI GCNGC 1 cut(s) 595
BmcAI AGTACT 2 cut(s) 241, 728
Bme1390I CCNGG 1 cut(s) 372
BmeT110I CYCGRG 1 cut(s) 75
BmrFI CCNGG 1 cut(s) 372
BmsI GCATC 2 cut(s) 196, 730
BpmI CTGGAG 1 cut(s) 601
BpuEI CTTGAG 4 cut(s) 6, 228, 256, 488
BsaAI YACGTR 1 cut(s) 252
BsaHI GRCGYC 1 cut(s) 619
BsaI GGTCTC 1 cut(s) 361
BsaJI CCNNGG 1 cut(s) 301
Bsc4I CCNNNNNNNGG 1 cut(s) 805
Bse1I ACTGG 1 cut(s) 584
BseBI CCWGG 1 cut(s) 372
BseDI CCNNGG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 805
BseMII CTCAG 1 cut(s) 854
BseNI ACTGG 1 cut(s) 584
BseRI GAGGAG 2 cut(s) 93, 737
BseXI GCAGC 1 cut(s) 580
BsgI GTGCAG 1 cut(s) 74
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 2 cut(s) 479, 707
BsiHKAI GWGCWC 1 cut(s) 320
BsiHKCI CYCGRG 1 cut(s) 75
BslFI GGGAC 1 cut(s) 155
BslI CCNNNNNNNGG 1 cut(s) 805
BsmAI GTCTC 2 cut(s) 361, 760
BsmFI GGGAC 1 cut(s) 155
BsnI GGCC 2 cut(s) 479, 707
Bso31I GGTCTC 1 cut(s) 361
BsoBI CYCGRG 1 cut(s) 75
Bsp1286I GDGCHC 1 cut(s) 320
Bsp1407I TGTACA 1 cut(s) 882
Bsp143I GATC 5 cut(s) 25, 118, 154, 458, 499
BspANI GGCC 2 cut(s) 479, 707
BspCNI CTCAG 1 cut(s) 853
BspFNI CGCG 1 cut(s) 168
BspQI GCTCTTC 1 cut(s) 821
BspTNI GGTCTC 1 cut(s) 361
BsrGI TGTACA 1 cut(s) 882
BsrI ACTGG 1 cut(s) 584
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 5 cut(s) 25, 118, 154, 458, 499
BssNI GRCGYC 1 cut(s) 619
Bst2UI CCWGG 1 cut(s) 372
Bst4CI ACNGT 2 cut(s) 454, 683
Bst6I CTCTTC 3 cut(s) 626, 798, 821
BstACI GRCGYC 1 cut(s) 619
BstAUI TGTACA 1 cut(s) 882
BstBAI YACGTR 1 cut(s) 252
BstC8I GCNNGC 5 cut(s) 69, 218, 399, 598, 762
BstDEI CTNAG 3 cut(s) 488, 653, 840
BstEII GGTNACC 1 cut(s) 269
BstFNI CGCG 1 cut(s) 168
BstKTI GATC 5 cut(s) 28, 121, 157, 461, 502
BstMAI GTCTC 2 cut(s) 361, 760
BstMBI GATC 5 cut(s) 25, 118, 154, 458, 499
BstMWI GCNNNNNNNGC 5 cut(s) 52, 491, 704, 761, 828
BstNI CCWGG 1 cut(s) 372
BstNSI RCATGY 1 cut(s) 600
BstPI GGTNACC 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 370
BstSFI CTRYAG 2 cut(s) 114, 911
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 1 cut(s) 580
BstX2I RGATCY 4 cut(s) 25, 118, 154, 499
BstYI RGATCY 4 cut(s) 25, 118, 154, 499
BsuRI GGCC 2 cut(s) 479, 707
BtsIMutI CAGTG 1 cut(s) 577
Cac8I GCNNGC 5 cut(s) 69, 218, 399, 598, 762
CseI GACGC 3 cut(s) 157, 174, 608
CsiI ACCWGGT 1 cut(s) 370
Csp6I GTAC 5 cut(s) 58, 240, 253, 727, 883
CviAII CATG 3 cut(s) 536, 597, 658
CviQI GTAC 5 cut(s) 58, 240, 253, 727, 883
DdeI CTNAG 3 cut(s) 488, 653, 840
DpnI GATC 5 cut(s) 27, 120, 156, 460, 501
DpnII GATC 5 cut(s) 25, 118, 154, 458, 499
Eam1104I CTCTTC 3 cut(s) 626, 798, 821
EarI CTCTTC 3 cut(s) 626, 798, 821
Ecl136II GAGCTC 1 cut(s) 318
Eco147I AGGCCT 1 cut(s) 479
Eco24I GRGCYC 1 cut(s) 320
Eco31I GGTCTC 1 cut(s) 361
Eco53kI GAGCTC 1 cut(s) 318
Eco57I CTGAAG 1 cut(s) 845
Eco88I CYCGRG 1 cut(s) 75
Eco91I GGTNACC 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 318
EcoO65I GGTNACC 1 cut(s) 269
EcoRII CCWGG 1 cut(s) 370
EcoT38I GRGCYC 1 cut(s) 320
FaeI CATG 3 cut(s) 539, 600, 661
FalI AAGNNNNNCTT 2 cut(s) 381, 413
FaqI GGGAC 1 cut(s) 155
FatI CATG 3 cut(s) 535, 596, 657
FauNDI CATATG 1 cut(s) 212
Fnu4HI GCNGC 1 cut(s) 594
FriOI GRGCYC 1 cut(s) 320
Fsp4HI GCNGC 1 cut(s) 594
FspBI CTAG 2 cut(s) 311, 861
GluI GCNGC 1 cut(s) 594
GsuI CTGGAG 1 cut(s) 601
HaeIII GGCC 2 cut(s) 479, 707
HgaI GACGC 3 cut(s) 157, 174, 608
Hin1I GRCGYC 1 cut(s) 619
Hin1II CATG 3 cut(s) 539, 600, 661
HincII GTYRAC 1 cut(s) 197
HindII GTYRAC 1 cut(s) 197
HinfI GANTC 5 cut(s) 92, 564, 649, 665, 888
HphI GGTGA 4 cut(s) 134, 281, 386, 797
Hpy166II GTNNAC 1 cut(s) 197
Hpy188I TCNGA 3 cut(s) 302, 339, 563
Hpy188III TCNNGA 4 cut(s) 23, 128, 263, 628
Hpy8I GTNNAC 1 cut(s) 197
Hpy99I CGWCG 3 cut(s) 129, 168, 531
HpyAV CCTTC 3 cut(s) 260, 447, 756
HpyCH4III ACNGT 2 cut(s) 454, 683
HpyCH4IV ACGT 3 cut(s) 37, 139, 251
HpyCH4V TGCA 7 cut(s) 31, 55, 71, 485, 550, 596, 743
HpyF10VI GCNNNNNNNGC 5 cut(s) 52, 491, 704, 761, 828
HpyF3I CTNAG 3 cut(s) 488, 653, 840
HpySE526I ACGT 3 cut(s) 37, 139, 251
Hsp92I GRCGYC 1 cut(s) 619
Hsp92II CATG 3 cut(s) 539, 600, 661
Kzo9I GATC 5 cut(s) 25, 118, 154, 458, 499
LguI GCTCTTC 1 cut(s) 821
LpnPI CCDG 9 cut(s) 288, 327, 357, 384, 565, 593, 836, 845, 863
Lsp1109I GCAGC 1 cut(s) 580
LweI GCATC 2 cut(s) 196, 730
MabI ACCWGGT 1 cut(s) 370
MaeI CTAG 2 cut(s) 311, 861
MaeII ACGT 3 cut(s) 37, 139, 251
MaeIII GTNAC 7 cut(s) 140, 269, 277, 288, 683, 785, 863
MalI GATC 5 cut(s) 27, 120, 156, 460, 501
MboI GATC 5 cut(s) 25, 118, 154, 458, 499
MflI RGATCY 4 cut(s) 25, 118, 154, 499
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 4 cut(s) 392, 623, 772, 895
MluI ACGCGT 1 cut(s) 166
MlyI GAGTC 1 cut(s) 674
MmeI TCCRAC 1 cut(s) 317
MseI TTAA 1 cut(s) 465
MslI CAYNNNNRTG 2 cut(s) 284, 878
MspR9I CCNGG 1 cut(s) 372
MvaI CCWGG 1 cut(s) 372
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 5 cut(s) 52, 491, 704, 761, 828
NdeI CATATG 1 cut(s) 212
NdeII GATC 5 cut(s) 25, 118, 154, 458, 499
NlaIII CATG 3 cut(s) 539, 600, 661
NmeAIII GCCGAG 1 cut(s) 824
NmuCI GTSAC 5 cut(s) 140, 269, 277, 683, 785
NspI RCATGY 1 cut(s) 600
PaeI GCATGC 1 cut(s) 600
PaeR7I CTCGAG 1 cut(s) 75
PceI AGGCCT 1 cut(s) 479
PciSI GCTCTTC 1 cut(s) 821
PcsI WCGNNNNNNNCGW 1 cut(s) 43
PfeI GAWTC 4 cut(s) 92, 564, 649, 888
PflFI GACNNNGTC 1 cut(s) 275
PkrI GCNGC 1 cut(s) 595
PleI GAGTC 1 cut(s) 673
PpsI GAGTC 1 cut(s) 673
Ppu21I YACGTR 1 cut(s) 252
Psp124BI GAGCTC 1 cut(s) 320
Psp6I CCWGG 1 cut(s) 370
PspEI GGTNACC 1 cut(s) 269
PspGI CCWGG 1 cut(s) 370
PspXI VCTCGAGB 1 cut(s) 75
PsuI RGATCY 4 cut(s) 25, 118, 154, 499
PsyI GACNNNGTC 1 cut(s) 275
RsaI GTAC 5 cut(s) 59, 241, 254, 728, 884
RsaNI GTAC 5 cut(s) 58, 240, 253, 727, 883
RseI CAYNNNNRTG 2 cut(s) 284, 878
SacI GAGCTC 1 cut(s) 320
SapI GCTCTTC 1 cut(s) 821
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 1 cut(s) 594
Sau3AI GATC 5 cut(s) 25, 118, 154, 458, 499
ScaI AGTACT 2 cut(s) 241, 728
SchI GAGTC 1 cut(s) 674
ScrFI CCNGG 1 cut(s) 372
SduI GDGCHC 1 cut(s) 320
SexAI ACCWGGT 1 cut(s) 370
SfaNI GCATC 2 cut(s) 196, 730
SfcI CTRYAG 2 cut(s) 114, 911
Sfr274I CTCGAG 1 cut(s) 75
SlaI CTCGAG 1 cut(s) 75
SmiMI CAYNNNNRTG 2 cut(s) 284, 878
SmlI CTYRAG 5 cut(s) 21, 75, 235, 243, 503
SmoI CTYRAG 5 cut(s) 21, 75, 235, 243, 503
SpeI ACTAGT 1 cut(s) 860
SphI GCATGC 1 cut(s) 600
Sse9I AATT 4 cut(s) 392, 623, 772, 895
SseBI AGGCCT 1 cut(s) 479
SspMI CTAG 2 cut(s) 311, 861
SstI GAGCTC 1 cut(s) 320
StuI AGGCCT 1 cut(s) 479
StyD4I CCNGG 1 cut(s) 370
TaaI ACNGT 2 cut(s) 454, 683
TaiI ACGT 3 cut(s) 40, 142, 254
TaqI TCGA 5 cut(s) 76, 127, 163, 262, 526
TasI AATT 4 cut(s) 392, 623, 772, 895
TatI WGTACW 4 cut(s) 57, 239, 726, 882
TfiI GAWTC 4 cut(s) 92, 564, 649, 888
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TscAI CASTG 1 cut(s) 584
TseFI GTSAC 5 cut(s) 140, 269, 277, 683, 785
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 5 cut(s) 140, 269, 277, 683, 785
TspDTI ATGAA 7 cut(s) 102, 483, 524, 592, 663, 862, 880
TspRI CASTG 1 cut(s) 584
Tth111I GACNNNGTC 1 cut(s) 275
XapI RAATTY 1 cut(s) 772
XceI RCATGY 1 cut(s) 600
XhoI CTCGAG 1 cut(s) 75
XspI CTAG 2 cut(s) 311, 861
ZrmI AGTACT 2 cut(s) 241, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.