MD15G1109900.v1.1

plastid-lipid-associated protein 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009105
Physical Location & Seq
Forward (+)
7695347 .. 7697374
2028 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1109900.v1.1.491

Sequence Viewer

Length: 744 bp
ATGGCCGCTGCCGTAGCCTCATCACTAGCCCTGTCAGCCTTCACCCTCAGAGGAGGCGCCGATCCCTCGAAACCCAAATTCCTCAACCTTTGCTCAAAGCCCACAAATTTGGCTTTGACCGTTGCACCCCAATCTCACAGTTGTCGGCGCAACTCTTTGAGAATATTGGCCTCTGTTTCCGTCTCCGATCCTGGAGTTCGTACCGGTCCCGAAGAGCTCTCTGCGTCTATTCTTTCCAAGGTGACGAATTCGGATAGAGGAGTTCTGCTGAAAGAGGAACAACAAAAAGAGGTAGCTAAGGAGGCTGAAGAATTGCAGAACTACTGTGTTAGCGAACCAGTGAAATGCCCTCTCATTTTCGGAGACTGGGATGTGGTGTACTGCTCAGTGCCAACATCACCCGGGGGTGGCTACAGAAGTACGTTGGGGCGCCTTTTTTTTAAAACAAAGGAGATGATTCAAGTTATTGAAGCTCCTGACATTGTCAAGAACAAAGTCTCCTTCTCCGTTTTCGGGTTCCTGGATGGAGAGGTCTCTTTAACAGGAAAGTTGAAGGCTCTGGATGATAAGTGGATCCAAGTTATATTTGAGCCCCCAGAACTCAAGGTTGGAGCCTTGGATTTCAGGTACGGCGGTGAAAGCGAGGTTAAACTCCAGATTACATACATAGATGACAAGGTCAGGTTGGGGAAGGGCTCTAGAGGCTCTCTATTTGTTTTCCAGAGACGCGGTAGTTCCACCTAA

Protein Analysis

248

Amino Acids

26.87

Weight (kDa)

8.74

Isoelectric Point (pI)

36.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 73 - 240 2.4e-13 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 56, 429
AccII CGCG 1 cut(s) 729
AciI CCGC 3 cut(s) 6, 633, 729
AclWI GGATC 4 cut(s) 56, 182, 568, 581
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 77, 106, 247
AcuI CTGAAG 1 cut(s) 327
AcyI GRCGYC 2 cut(s) 57, 430
AfaI GTAC 4 cut(s) 202, 380, 421, 629
AfiI CCNNNNNNNGG 2 cut(s) 407, 513
AgeI ACCGGT 1 cut(s) 203
AgsI TTSAA 3 cut(s) 461, 470, 553
AjnI CCWGG 2 cut(s) 190, 519
AjuI GAANNNNNNNTTGG 2 cut(s) 591, 623
AloI GAACNNNNNNTCC 2 cut(s) 482, 514
AluBI AGCT 3 cut(s) 217, 296, 473
AluI AGCT 3 cut(s) 217, 296, 473
Alw21I GWGCWC 1 cut(s) 219
Alw26I GTCTC 5 cut(s) 187, 357, 502, 538, 718
AlwI GGATC 4 cut(s) 56, 182, 568, 581
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 2 cut(s) 3, 168
ApeKI GCWGC 1 cut(s) 8
ApoI RAATTY 3 cut(s) 77, 106, 247
AsiGI ACCGGT 1 cut(s) 203
AspLEI GCGC 3 cut(s) 59, 150, 432
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 2 cut(s) 402, 403
AsuHPI GGTGA 4 cut(s) 34, 253, 390, 647
AvaI CYCGRG 1 cut(s) 401
AvaII GGWCC 1 cut(s) 206
BaeI ACNNNNGTAYC 2 cut(s) 619, 652
BamHI GGATCC 1 cut(s) 573
BanI GGYRCC 2 cut(s) 56, 429
BanII GRGCYC 3 cut(s) 219, 594, 698
Bbv12I GWGCWC 1 cut(s) 219
BccI CCATC 1 cut(s) 518
BceAI ACGGC 1 cut(s) 646
BciT130I CCWGG 2 cut(s) 192, 521
BcnI CCSGG 2 cut(s) 402, 403
BcoDI GTCTC 5 cut(s) 187, 357, 502, 538, 718
BfaI CTAG 2 cut(s) 26, 699
BfmI CTRYAG 1 cut(s) 412
BfoI RGCGCY 2 cut(s) 60, 433
BisI GCNGC 2 cut(s) 6, 9
BlsI GCNGC 2 cut(s) 7, 10
Bme1390I CCNGG 4 cut(s) 192, 402, 403, 521
Bme18I GGWCC 1 cut(s) 206
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 6 cut(s) 58, 208, 431, 518, 575, 613
BmrFI CCNGG 4 cut(s) 192, 402, 403, 521
BmrI ACTGGG 1 cut(s) 376
BmuI ACTGGG 1 cut(s) 376
BpmI CTGGAG 2 cut(s) 213, 638
Bpu10I CCTNAGC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 587
BpuMI CCSGG 2 cut(s) 402, 403
BsaHI GRCGYC 2 cut(s) 57, 430
BsaI GGTCTC 1 cut(s) 538
BsaJI CCNNGG 4 cut(s) 237, 401, 402, 615
BsaWI WCCGGW 1 cut(s) 203
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 407, 513
Bse118I RCCGGY 1 cut(s) 203
Bse1I ACTGG 2 cut(s) 338, 371
BseBI CCWGG 2 cut(s) 192, 521
BseDI CCNNGG 4 cut(s) 237, 401, 402, 615
BseGI GGATG 3 cut(s) 376, 529, 568
BseLI CCNNNNNNNGG 2 cut(s) 407, 513
BseMII CTCAG 2 cut(s) 61, 399
BseNI ACTGG 2 cut(s) 338, 371
BseRI GAGGAG 2 cut(s) 66, 273
Bsh1236I CGCG 1 cut(s) 729
BshFI GGCC 2 cut(s) 5, 170
BshNI GGYRCC 2 cut(s) 56, 429
BshTI ACCGGT 1 cut(s) 203
BsiHKAI GWGCWC 1 cut(s) 219
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 2 cut(s) 204, 402
BslFI GGGAC 1 cut(s) 192
BslI CCNNNNNNNGG 2 cut(s) 407, 513
BsmAI GTCTC 5 cut(s) 187, 357, 502, 538, 718
BsmBI CGTCTC 2 cut(s) 187, 718
BsmFI GGGAC 1 cut(s) 192
BsnI GGCC 2 cut(s) 5, 170
Bso31I GGTCTC 1 cut(s) 538
BsoBI CYCGRG 1 cut(s) 401
Bsp1286I GDGCHC 3 cut(s) 219, 594, 698
Bsp143I GATC 3 cut(s) 61, 187, 573
BspACI CCGC 3 cut(s) 6, 633, 729
BspANI GGCC 2 cut(s) 5, 170
BspCNI CTCAG 2 cut(s) 60, 398
BspFNI CGCG 1 cut(s) 729
BspLI GGNNCC 6 cut(s) 58, 208, 431, 518, 575, 613
BspPI GGATC 4 cut(s) 56, 182, 568, 581
BspQI GCTCTTC 1 cut(s) 207
BspT107I GGYRCC 2 cut(s) 56, 429
BspTNI GGTCTC 1 cut(s) 538
BsrFI RCCGGY 1 cut(s) 203
BsrI ACTGG 2 cut(s) 338, 371
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 4 cut(s) 237, 401, 402, 615
BssMI GATC 3 cut(s) 61, 187, 573
BssNI GRCGYC 2 cut(s) 57, 430
BssT1I CCWWGG 2 cut(s) 237, 615
Bst2UI CCWGG 2 cut(s) 192, 521
Bst4CI ACNGT 3 cut(s) 121, 140, 326
Bst6I CTCTTC 1 cut(s) 207
BstACI GRCGYC 2 cut(s) 57, 430
BstDEI CTNAG 3 cut(s) 47, 297, 385
BstF5I GGATG 3 cut(s) 376, 529, 568
BstFNI CGCG 1 cut(s) 729
BstH2I RGCGCY 2 cut(s) 60, 433
BstHHI GCGC 3 cut(s) 59, 150, 432
BstKTI GATC 3 cut(s) 64, 190, 576
BstMAI GTCTC 5 cut(s) 187, 357, 502, 538, 718
BstMBI GATC 3 cut(s) 61, 187, 573
BstMWI GCNNNNNNNGC 5 cut(s) 14, 35, 302, 639, 702
BstNI CCWGG 2 cut(s) 192, 521
BstSCI CCNGG 4 cut(s) 190, 400, 401, 519
BstSFI CTRYAG 1 cut(s) 412
BstUI CGCG 1 cut(s) 729
BstX2I RGATCY 1 cut(s) 573
BstXI CCANNNNNNTGG 1 cut(s) 109
BstYI RGATCY 1 cut(s) 573
BsuRI GGCC 2 cut(s) 5, 170
BtsCI GGATG 3 cut(s) 376, 529, 568
BtsIMutI CAGTG 2 cut(s) 345, 393
CfoI GCGC 3 cut(s) 59, 150, 432
Cfr10I RCCGGY 1 cut(s) 203
Cfr13I GGNCC 1 cut(s) 206
Cfr9I CCCGGG 1 cut(s) 401
CseI GACGC 2 cut(s) 213, 735
Csp6I GTAC 4 cut(s) 201, 379, 420, 628
CspAI ACCGGT 1 cut(s) 203
CviQI GTAC 4 cut(s) 201, 379, 420, 628
DdeI CTNAG 3 cut(s) 47, 297, 385
DinI GGCGCC 2 cut(s) 58, 431
DpnI GATC 3 cut(s) 63, 189, 575
DpnII GATC 3 cut(s) 61, 187, 573
DraI TTTAAA 1 cut(s) 442
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Ecl136II GAGCTC 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 237, 615
Eco24I GRGCYC 3 cut(s) 219, 594, 698
Eco31I GGTCTC 1 cut(s) 538
Eco47I GGWCC 1 cut(s) 206
Eco53kI GAGCTC 1 cut(s) 217
Eco57I CTGAAG 1 cut(s) 327
Eco88I CYCGRG 1 cut(s) 401
EcoICRI GAGCTC 1 cut(s) 217
EcoRI GAATTC 1 cut(s) 247
EcoRII CCWGG 2 cut(s) 190, 519
EcoT14I CCWWGG 2 cut(s) 237, 615
EcoT38I GRGCYC 3 cut(s) 219, 594, 698
EgeI GGCGCC 2 cut(s) 58, 431
EheI GGCGCC 2 cut(s) 58, 431
ErhI CCWWGG 2 cut(s) 237, 615
Esp3I CGTCTC 2 cut(s) 187, 718
FaiI YATR 3 cut(s) 584, 664, 668
FaqI GGGAC 1 cut(s) 192
Fnu4HI GCNGC 2 cut(s) 6, 9
FokI GGATG 3 cut(s) 383, 536, 575
FriOI GRGCYC 3 cut(s) 219, 594, 698
Fsp4HI GCNGC 2 cut(s) 6, 9
FspBI CTAG 2 cut(s) 26, 699
GlaI GCGC 3 cut(s) 58, 149, 431
GluI GCNGC 2 cut(s) 6, 9
GsuI CTGGAG 2 cut(s) 213, 638
HaeII RGCGCY 2 cut(s) 60, 433
HaeIII GGCC 2 cut(s) 5, 170
HapII CCGG 2 cut(s) 204, 402
HgaI GACGC 2 cut(s) 213, 735
HhaI GCGC 3 cut(s) 59, 150, 432
Hin1I GRCGYC 2 cut(s) 57, 430
Hin6I GCGC 3 cut(s) 57, 148, 430
HinP1I GCGC 3 cut(s) 57, 148, 430
HinfI GANTC 1 cut(s) 457
HpaII CCGG 2 cut(s) 204, 402
HphI GGTGA 4 cut(s) 34, 253, 390, 647
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 4 cut(s) 50, 187, 253, 362
Hpy188III TCNNGA 7 cut(s) 209, 476, 487, 560, 655, 699, 721
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 4 cut(s) 49, 511, 547, 685
HpyCH4III ACNGT 3 cut(s) 121, 140, 326
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 2 cut(s) 125, 316
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 35, 302, 639, 702
HpyF3I CTNAG 3 cut(s) 47, 297, 385
HpySE526I ACGT 1 cut(s) 422
Hsp92I GRCGYC 2 cut(s) 57, 430
HspAI GCGC 3 cut(s) 57, 148, 430
KasI GGCGCC 2 cut(s) 56, 429
Kzo9I GATC 3 cut(s) 61, 187, 573
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 2 cut(s) 478, 611
MaeI CTAG 2 cut(s) 26, 699
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 1 cut(s) 241
MalI GATC 3 cut(s) 63, 189, 575
MboI GATC 3 cut(s) 61, 187, 573
MboII GAAGA 2 cut(s) 224, 320
MflI RGATCY 1 cut(s) 573
MhlI GDGCHC 3 cut(s) 219, 594, 698
MluCI AATT 4 cut(s) 77, 106, 247, 311
Mly113I GGCGCC 2 cut(s) 57, 430
MmeI TCCRAC 1 cut(s) 589
MseI TTAA 3 cut(s) 441, 539, 648
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 204, 402
MspR9I CCNGG 4 cut(s) 192, 402, 403, 521
MvaI CCWGG 2 cut(s) 192, 521
MvnI CGCG 1 cut(s) 729
MwoI GCNNNNNNNGC 5 cut(s) 14, 35, 302, 639, 702
NarI GGCGCC 2 cut(s) 57, 430
NciI CCSGG 2 cut(s) 402, 403
NdeII GATC 3 cut(s) 61, 187, 573
NlaIV GGNNCC 6 cut(s) 58, 208, 431, 518, 575, 613
NmuCI GTSAC 1 cut(s) 241
PciSI GCTCTTC 1 cut(s) 207
PfeI GAWTC 1 cut(s) 457
PflFI GACNNNGTC 2 cut(s) 482, 677
PfoI TCCNGGA 2 cut(s) 190, 519
PinAI ACCGGT 1 cut(s) 203
PkrI GCNGC 2 cut(s) 7, 10
PluTI GGCGCC 2 cut(s) 60, 433
Psp124BI GAGCTC 1 cut(s) 219
Psp6I CCWGG 2 cut(s) 190, 519
PspGI CCWGG 2 cut(s) 190, 519
PspN4I GGNNCC 6 cut(s) 58, 208, 431, 518, 575, 613
PspPI GGNCC 1 cut(s) 206
PsuI RGATCY 1 cut(s) 573
PsyI GACNNNGTC 2 cut(s) 482, 677
RsaI GTAC 4 cut(s) 202, 380, 421, 629
RsaNI GTAC 4 cut(s) 201, 379, 420, 628
SacI GAGCTC 1 cut(s) 219
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 3 cut(s) 441, 539, 648
SatI GCNGC 2 cut(s) 6, 9
Sau3AI GATC 3 cut(s) 61, 187, 573
Sau96I GGNCC 1 cut(s) 206
ScrFI CCNGG 4 cut(s) 192, 402, 403, 521
SduI GDGCHC 3 cut(s) 219, 594, 698
SfcI CTRYAG 1 cut(s) 412
SfoI GGCGCC 2 cut(s) 58, 431
SinI GGWCC 1 cut(s) 206
SmaI CCCGGG 1 cut(s) 403
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
Sse9I AATT 4 cut(s) 77, 106, 247, 311
SsiI CCGC 3 cut(s) 6, 633, 729
SspDI GGCGCC 2 cut(s) 56, 429
SspI AATATT 1 cut(s) 165
SspMI CTAG 2 cut(s) 26, 699
SstI GAGCTC 1 cut(s) 219
StyD4I CCNGG 4 cut(s) 190, 400, 401, 519
StyI CCWWGG 2 cut(s) 237, 615
TaaI ACNGT 3 cut(s) 121, 140, 326
TaiI ACGT 1 cut(s) 425
TaqI TCGA 1 cut(s) 68
TasI AATT 4 cut(s) 77, 106, 247, 311
TatI WGTACW 1 cut(s) 378
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 1 cut(s) 457
Tru1I TTAA 3 cut(s) 441, 539, 648
Tru9I TTAA 3 cut(s) 441, 539, 648
TscAI CASTG 2 cut(s) 345, 393
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 1 cut(s) 8
Tsp45I GTSAC 1 cut(s) 241
TspGWI ACGGA 2 cut(s) 169, 496
TspMI CCCGGG 1 cut(s) 401
TspRI CASTG 2 cut(s) 345, 393
Tth111I GACNNNGTC 2 cut(s) 482, 677
VpaK11BI GGWCC 1 cut(s) 206
XapI RAATTY 3 cut(s) 77, 106, 247
XbaI TCTAGA 1 cut(s) 698
XmaI CCCGGG 1 cut(s) 401
XspI CTAG 2 cut(s) 26, 699
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.