Rroxscaffold_7G00156960

plastid-lipid-associated protein 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0009105
Physical Location & Seq
Reverse (-)
827282 .. 829275
1994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00156960.1

Sequence Viewer

Length: 801 bp
ATGTACTCTGCCCCTGCAAAACCCAATCTGGTTTGGAAGCTCAGCATCATCAACTTCATGGCTGCTGCTCTCGCTTCTTCACTAGCCTTATCGTGCTCTACACTCAGAGGAGGCTCCGATCCCTCAAAACCCAGGTTCCCTAATCTCTGCGCAAAGCCCACTAATTTGGGTTTAGCTGTTTCGCCTCACTCTCATTCTCGTAACCTCAAAAAGTTGAGAATCTCGGCTACTGTCTCGGTCTCTAACCCGGAATTACGGACCGGTCCCGACGAGCTCGCTGCTTCTATTCTCTCCAAGGTGATAGATTCGGACAGAGGAGTTTTGCTGGAAAAGGAACAACATAAGGAGGTAGCTCAAGTGGCCGAGGCATTGCAGAACTACTGTGTTAGTGAACCAGTGAAATGCCCTCTAATTTTCGGAGATTGGGATGTGCTGTACTGTTCAGTGCCAACGTCGCCTGGGGGTGGCTATAGGAGTACACTGGGCCGTCTTGTTTTTAAAACAAAGGAGATGATTCAAGTTATTGAAGCTCCTGACATTGTTAAAAACAAGGTGTCCTTCTCTGCTTTTGGGTTCTTGGATGGAGAAGTCTCTTTAACAGGAAAGTTGAAGGCTCTGGATGATAAATGGGTTCAAGTTATTTTTGAACCACCAGAATTGAAGGTTGGAGCTTTGGACTTTCGATATGGCGGTGAAAGTGAGGTTAAACTCCAGATCACATATATAGATGAGAATATCAGGCTGGGGAAAGGCTCTAGAGGTTCACTGTTTGTTTTCAGAAGACAAGGTAAATCTAACTGA

Protein Analysis

266

Amino Acids

29.01

Weight (kDa)

9.12

Isoelectric Point (pI)

32.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 93 - 259 7.2e-15 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 151
AciI CCGC 1 cut(s) 690
AclWI GGATC 1 cut(s) 113
AcoI YGGCCR 1 cut(s) 360
AfaI GTAC 3 cut(s) 5, 437, 478
AfiI CCNNNNNNNGG 1 cut(s) 464
AgeI ACCGGT 1 cut(s) 260
AgsI TTSAA 6 cut(s) 518, 527, 610, 635, 647, 661
AjnI CCWGG 2 cut(s) 131, 457
AjuI GAANNNNNNNTTGG 2 cut(s) 648, 680
AluBI AGCT 6 cut(s) 40, 176, 274, 353, 530, 671
AluI AGCT 6 cut(s) 40, 176, 274, 353, 530, 671
Alw21I GWGCWC 2 cut(s) 98, 276
Alw26I GTCTC 3 cut(s) 238, 244, 595
AlwI GGATC 1 cut(s) 113
AoxI GGCC 2 cut(s) 360, 484
ApeKI GCWGC 3 cut(s) 62, 65, 278
AsiGI ACCGGT 1 cut(s) 260
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 3 cut(s) 258, 263, 484
AsuC2I CCSGG 1 cut(s) 248
AsuHPI GGTGA 2 cut(s) 310, 704
AvaII GGWCC 2 cut(s) 258, 263
BanII GRGCYC 1 cut(s) 276
BbsI GAAGAC 1 cut(s) 787
Bbv12I GWGCWC 2 cut(s) 98, 276
BbvI GCAGC 3 cut(s) 49, 52, 265
BccI CCATC 1 cut(s) 575
BceAI ACGGC 1 cut(s) 471
BcgI CGANNNNNNTGC 2 cut(s) 260, 294
BciT130I CCWGG 2 cut(s) 133, 459
BcnI CCSGG 1 cut(s) 248
BcoDI GTCTC 3 cut(s) 238, 244, 595
BfaI CTAG 2 cut(s) 83, 756
BfmI CTRYAG 1 cut(s) 469
BisI GCNGC 3 cut(s) 63, 66, 279
BlpI GCTNAGC 1 cut(s) 41
BlsI GCNGC 3 cut(s) 64, 67, 280
Bme1390I CCNGG 3 cut(s) 133, 248, 459
Bme18I GGWCC 2 cut(s) 258, 263
BmgT120I GGNCC 3 cut(s) 258, 263, 484
BmiI GGNNCC 3 cut(s) 115, 137, 265
BmrFI CCNGG 3 cut(s) 133, 248, 459
BmrI ACTGGG 1 cut(s) 491
BmsI GCATC 1 cut(s) 54
BmuI ACTGGG 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 787
BpmI CTGGAG 1 cut(s) 695
Bpu1102I GCTNAGC 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 339
BpuMI CCSGG 1 cut(s) 248
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 4 cut(s) 131, 294, 363, 458
BsaWI WCCGGW 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 464
Bse118I RCCGGY 1 cut(s) 260
Bse1I ACTGG 2 cut(s) 395, 486
Bse3DI GCAATG 1 cut(s) 368
BseBI CCWGG 2 cut(s) 133, 459
BseDI CCNNGG 4 cut(s) 131, 294, 363, 458
BseGI GGATG 3 cut(s) 433, 586, 625
BseLI CCNNNNNNNGG 1 cut(s) 464
BseMI GCAATG 1 cut(s) 368
BseMII CTCAG 2 cut(s) 55, 118
BseNI ACTGG 2 cut(s) 395, 486
BseRI GAGGAG 2 cut(s) 123, 330
BseXI GCAGC 3 cut(s) 49, 52, 265
BseYI CCCAGC 1 cut(s) 742
BshFI GGCC 2 cut(s) 362, 486
BshTI ACCGGT 1 cut(s) 260
BsiHKAI GWGCWC 2 cut(s) 98, 276
BsiSI CCGG 2 cut(s) 248, 261
BslFI GGGAC 1 cut(s) 249
BslI CCNNNNNNNGG 1 cut(s) 464
BsmAI GTCTC 3 cut(s) 238, 244, 595
BsmFI GGGAC 1 cut(s) 249
BsnI GGCC 2 cut(s) 362, 486
Bso31I GGTCTC 1 cut(s) 244
Bsp1286I GDGCHC 2 cut(s) 98, 276
Bsp143I GATC 2 cut(s) 118, 714
Bsp1720I GCTNAGC 1 cut(s) 41
BspACI CCGC 1 cut(s) 690
BspANI GGCC 2 cut(s) 362, 486
BspCNI CTCAG 2 cut(s) 54, 117
BspLI GGNNCC 3 cut(s) 115, 137, 265
BspPI GGATC 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 260
BsrI ACTGG 2 cut(s) 395, 486
BssAI RCCGGY 1 cut(s) 260
BssECI CCNNGG 4 cut(s) 131, 294, 363, 458
BssMI GATC 2 cut(s) 118, 714
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 2 cut(s) 133, 459
Bst4CI ACNGT 4 cut(s) 232, 383, 440, 768
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 41, 104
BstF5I GGATG 3 cut(s) 433, 586, 625
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 2 cut(s) 121, 717
BstMAI GTCTC 3 cut(s) 238, 244, 595
BstMBI GATC 2 cut(s) 118, 714
BstMWI GCNNNNNNNGC 3 cut(s) 71, 359, 454
BstNI CCWGG 2 cut(s) 133, 459
BstSCI CCNGG 3 cut(s) 131, 246, 457
BstSFI CTRYAG 1 cut(s) 469
BstV1I GCAGC 3 cut(s) 49, 52, 265
BstV2I GAAGAC 1 cut(s) 787
BstXI CCANNNNNNTGG 1 cut(s) 166
BsuRI GGCC 2 cut(s) 362, 486
BtsCI GGATG 3 cut(s) 433, 586, 625
BtsIMutI CAGTG 4 cut(s) 402, 450, 479, 764
Cac8I GCNNGC 1 cut(s) 276
CfoI GCGC 1 cut(s) 152
Cfr10I RCCGGY 1 cut(s) 260
Cfr13I GGNCC 3 cut(s) 258, 263, 484
CpoI CGGWCCG 1 cut(s) 258
Csp6I GTAC 3 cut(s) 4, 436, 477
CspAI ACCGGT 1 cut(s) 260
CspCI CAANNNNNGTGG 2 cut(s) 639, 674
CspI CGGWCCG 1 cut(s) 258
CviAII CATG 1 cut(s) 58
CviQI GTAC 3 cut(s) 4, 436, 477
DdeI CTNAG 2 cut(s) 41, 104
DpnI GATC 2 cut(s) 120, 716
DpnII GATC 2 cut(s) 118, 714
DraI TTTAAA 1 cut(s) 499
EaeI YGGCCR 1 cut(s) 360
Ecl136II GAGCTC 1 cut(s) 274
Eco130I CCWWGG 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 276
Eco31I GGTCTC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 258, 263
Eco53kI GAGCTC 1 cut(s) 274
EcoICRI GAGCTC 1 cut(s) 274
EcoRII CCWGG 2 cut(s) 131, 457
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 276
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 1 cut(s) 61
FaiI YATR 7 cut(s) 59, 342, 471, 687, 721, 723, 725
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 1 cut(s) 249
FatI CATG 1 cut(s) 57
Fnu4HI GCNGC 3 cut(s) 63, 66, 279
FokI GGATG 3 cut(s) 440, 593, 632
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 3 cut(s) 63, 66, 279
FspBI CTAG 2 cut(s) 83, 756
FspI TGCGCA 1 cut(s) 151
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 63, 66, 279
GsaI CCCAGC 1 cut(s) 746
GsuI CTGGAG 1 cut(s) 695
HaeIII GGCC 2 cut(s) 362, 486
HapII CCGG 2 cut(s) 248, 261
HhaI GCGC 1 cut(s) 152
Hin1II CATG 1 cut(s) 61
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 3 cut(s) 219, 305, 514
HpaII CCGG 2 cut(s) 248, 261
HphI GGTGA 2 cut(s) 310, 704
Hpy166II GTNNAC 3 cut(s) 392, 479, 764
Hpy188I TCNGA 5 cut(s) 107, 118, 310, 419, 779
Hpy188III TCNNGA 5 cut(s) 266, 533, 617, 712, 756
Hpy8I GTNNAC 3 cut(s) 392, 479, 764
Hpy99I CGWCG 2 cut(s) 272, 457
HpyAV CCTTC 3 cut(s) 568, 604, 655
HpyCH4III ACNGT 4 cut(s) 232, 383, 440, 768
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 2 cut(s) 17, 373
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 359, 454
HpyF3I CTNAG 2 cut(s) 41, 104
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 1 cut(s) 61
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 2 cut(s) 118, 714
LmnI GCTCC 3 cut(s) 119, 535, 668
Lsp1109I GCAGC 3 cut(s) 49, 52, 265
LweI GCATC 1 cut(s) 54
MaeI CTAG 2 cut(s) 83, 756
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 1 cut(s) 200
MalI GATC 2 cut(s) 120, 716
MboI GATC 2 cut(s) 118, 714
MboII GAAGA 2 cut(s) 69, 792
MhlI GDGCHC 2 cut(s) 98, 276
MluCI AATT 4 cut(s) 163, 251, 411, 656
MmeI TCCRAC 1 cut(s) 646
MseI TTAA 4 cut(s) 498, 543, 596, 705
MspI CCGG 2 cut(s) 248, 261
MspR9I CCNGG 3 cut(s) 133, 248, 459
MvaI CCWGG 2 cut(s) 133, 459
MwoI GCNNNNNNNGC 3 cut(s) 71, 359, 454
NciI CCSGG 1 cut(s) 248
NdeII GATC 2 cut(s) 118, 714
NlaIII CATG 1 cut(s) 61
NlaIV GGNNCC 3 cut(s) 115, 137, 265
NmeAIII GCCGAG 2 cut(s) 203, 388
NsbI TGCGCA 1 cut(s) 151
PfeI GAWTC 3 cut(s) 219, 305, 514
PinAI ACCGGT 1 cut(s) 260
PkrI GCNGC 3 cut(s) 64, 67, 280
Psp124BI GAGCTC 1 cut(s) 276
Psp6I CCWGG 2 cut(s) 131, 457
PspFI CCCAGC 1 cut(s) 742
PspGI CCWGG 2 cut(s) 131, 457
PspN4I GGNNCC 3 cut(s) 115, 137, 265
PspPI GGNCC 3 cut(s) 258, 263, 484
RsaI GTAC 3 cut(s) 5, 437, 478
RsaNI GTAC 3 cut(s) 4, 436, 477
Rsr2I CGGWCCG 1 cut(s) 258
RsrII CGGWCCG 1 cut(s) 258
SacI GAGCTC 1 cut(s) 276
SaqAI TTAA 4 cut(s) 498, 543, 596, 705
SatI GCNGC 3 cut(s) 63, 66, 279
Sau3AI GATC 2 cut(s) 118, 714
Sau96I GGNCC 3 cut(s) 258, 263, 484
ScrFI CCNGG 3 cut(s) 133, 248, 459
SduI GDGCHC 2 cut(s) 98, 276
SfaNI GCATC 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 2 cut(s) 258, 263
SmlI CTYRAG 1 cut(s) 354
SmoI CTYRAG 1 cut(s) 354
Sse9I AATT 4 cut(s) 163, 251, 411, 656
SsiI CCGC 1 cut(s) 690
SspMI CTAG 2 cut(s) 83, 756
SstI GAGCTC 1 cut(s) 276
StyD4I CCNGG 3 cut(s) 131, 246, 457
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 4 cut(s) 232, 383, 440, 768
TaiI ACGT 1 cut(s) 455
TaqI TCGA 1 cut(s) 682
TaqII GACCGA 1 cut(s) 226
TasI AATT 4 cut(s) 163, 251, 411, 656
TatI WGTACW 3 cut(s) 3, 435, 476
TfiI GAWTC 3 cut(s) 219, 305, 514
Tru1I TTAA 4 cut(s) 498, 543, 596, 705
Tru9I TTAA 4 cut(s) 498, 543, 596, 705
TscAI CASTG 4 cut(s) 402, 450, 486, 771
TseI GCWGC 3 cut(s) 62, 65, 278
TspDTI ATGAA 1 cut(s) 46
TspGWI ACGGA 1 cut(s) 271
TspRI CASTG 4 cut(s) 402, 450, 486, 771
VpaK11BI GGWCC 2 cut(s) 258, 263
XbaI TCTAGA 1 cut(s) 755
XspI CTAG 2 cut(s) 83, 756
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.