Rh6BG521900

plastid-lipid-associated protein 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009105
Physical Location & Seq
Forward (+)
71507973 .. 71512145
4173 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG521900.1

Sequence Viewer

Length: 516 bp
ATGCAAAGTGAGGTGATAGATTCGGACAGAGGAGTTTTGCTGGAAAAGGAACAACATAAGGAGGTAGCTCAAGTGGCCGAGGCATTGCAGAACTACTGTGTTAGTGAACCAGTGAAATGCCCTCTAATTTTCGGAGATTGGGATGTGCTGTACTGTTCAGTGCCAACATCGCCTGGGGGTGGCTATAGGAGTACACTTGGCCGCCTTGTTTTTAAAACGAAGGAGATGATTCAAGTTATTGAAGCTCCTGACATTGTCAAAAACAAGGTGTCCTTCTCTGCTTTTGGGTTCTTGGATGGCGAAGTCTCTTTAACAGGAAAGTTGAAGGCTCTGGATGATAAATGGGTTCAAGTTATTTTTGAACCACCAGAATTGAAGGTTGGAGCTTTGGAGTTCCGATATGGCGGTGAAAGTGAGGTTAAACTCCAGATCACATATATAGATGAGAATATCAGGCTGGGGAAAGGCTCTAGAGGTTCACTGTTTGTTTTCAGAAGACAAGGTAAATCTAATTGA

Protein Analysis

171

Amino Acids

19.08

Weight (kDa)

5.57

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP_fibrillin PF04755 4 - 164 9.8e-15 PAP_fibrillin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 202, 405
AcoI YGGCCR 2 cut(s) 75, 199
AfaI GTAC 2 cut(s) 152, 193
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 6 cut(s) 233, 242, 325, 350, 362, 376
AjnI CCWGG 1 cut(s) 172
AjuI GAANNNNNNNTTGG 2 cut(s) 363, 395
AluBI AGCT 3 cut(s) 68, 245, 386
AluI AGCT 3 cut(s) 68, 245, 386
Alw26I GTCTC 1 cut(s) 310
AoxI GGCC 2 cut(s) 75, 199
AsuHPI GGTGA 2 cut(s) 25, 419
BbsI GAAGAC 1 cut(s) 502
BccI CCATC 1 cut(s) 290
BciT130I CCWGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 1 cut(s) 471
BfmI CTRYAG 1 cut(s) 184
BisI GCNGC 1 cut(s) 202
BlsI GCNGC 1 cut(s) 203
Bme1390I CCNGG 1 cut(s) 174
BmrFI CCNGG 1 cut(s) 174
BpiI GAAGAC 1 cut(s) 502
BpmI CTGGAG 1 cut(s) 410
BpuEI CTTGAG 1 cut(s) 54
BsaJI CCNNGG 2 cut(s) 78, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 110
Bse3DI GCAATG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 174
BseDI CCNNGG 2 cut(s) 78, 173
BseGI GGATG 3 cut(s) 148, 301, 340
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMI GCAATG 1 cut(s) 83
BseNI ACTGG 1 cut(s) 110
BseRI GAGGAG 1 cut(s) 45
BseYI CCCAGC 1 cut(s) 457
BshFI GGCC 2 cut(s) 77, 201
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 310
BsnI GGCC 2 cut(s) 77, 201
Bsp143I GATC 1 cut(s) 429
BspACI CCGC 2 cut(s) 202, 405
BspANI GGCC 2 cut(s) 77, 201
BsrDI GCAATG 1 cut(s) 83
BsrI ACTGG 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 78, 173
BssMI GATC 1 cut(s) 429
Bst2UI CCWGG 1 cut(s) 174
Bst4CI ACNGT 3 cut(s) 98, 155, 483
BstF5I GGATG 3 cut(s) 148, 301, 340
BstKTI GATC 1 cut(s) 432
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 1 cut(s) 429
BstMWI GCNNNNNNNGC 2 cut(s) 74, 169
BstNI CCWGG 1 cut(s) 174
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 184
BstV2I GAAGAC 1 cut(s) 502
BsuRI GGCC 2 cut(s) 77, 201
BtgZI GCGATG 1 cut(s) 153
BtsCI GGATG 3 cut(s) 148, 301, 340
BtsIMutI CAGTG 3 cut(s) 117, 165, 479
Csp6I GTAC 2 cut(s) 151, 192
CspCI CAANNNNNGTGG 2 cut(s) 354, 389
CviJI RGCY 9 cut(s) 68, 77, 183, 201, 245, 329, 386, 457, 468
CviKI_1 RGCY 9 cut(s) 68, 77, 183, 201, 245, 329, 386, 457, 468
CviQI GTAC 2 cut(s) 151, 192
DpnI GATC 1 cut(s) 431
DpnII GATC 1 cut(s) 429
DraI TTTAAA 1 cut(s) 214
EaeI YGGCCR 2 cut(s) 75, 199
EcoRII CCWGG 1 cut(s) 172
FaiI YATR 6 cut(s) 57, 186, 402, 436, 438, 440
FalI AAGNNNNNCTT 2 cut(s) 257, 289
Fnu4HI GCNGC 1 cut(s) 202
FokI GGATG 3 cut(s) 155, 308, 347
Fsp4HI GCNGC 1 cut(s) 202
FspBI CTAG 1 cut(s) 471
GluI GCNGC 1 cut(s) 202
GsaI CCCAGC 1 cut(s) 461
GsuI CTGGAG 1 cut(s) 410
HaeIII GGCC 2 cut(s) 77, 201
HinfI GANTC 2 cut(s) 20, 229
HphI GGTGA 2 cut(s) 25, 419
Hpy166II GTNNAC 3 cut(s) 107, 194, 479
Hpy188I TCNGA 4 cut(s) 25, 134, 398, 494
Hpy188III TCNNGA 4 cut(s) 248, 332, 427, 471
Hpy8I GTNNAC 3 cut(s) 107, 194, 479
HpyAV CCTTC 4 cut(s) 214, 283, 319, 370
HpyCH4III ACNGT 3 cut(s) 98, 155, 483
HpyCH4V TGCA 2 cut(s) 4, 88
HpyF10VI GCNNNNNNNGC 2 cut(s) 74, 169
Kzo9I GATC 1 cut(s) 429
LmnI GCTCC 2 cut(s) 250, 383
MaeI CTAG 1 cut(s) 471
MalI GATC 1 cut(s) 431
MboI GATC 1 cut(s) 429
MboII GAAGA 1 cut(s) 507
MluCI AATT 3 cut(s) 126, 371, 511
MmeI TCCRAC 1 cut(s) 361
MnlI CCTC 7 cut(s) 4, 23, 55, 73, 132, 409, 467
MseI TTAA 3 cut(s) 213, 311, 420
MspR9I CCNGG 1 cut(s) 174
MvaI CCWGG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 74, 169
NdeII GATC 1 cut(s) 429
NmeAIII GCCGAG 1 cut(s) 103
PfeI GAWTC 2 cut(s) 20, 229
PflFI GACNNNGTC 1 cut(s) 254
PkrI GCNGC 1 cut(s) 203
Psp6I CCWGG 1 cut(s) 172
PspFI CCCAGC 1 cut(s) 457
PspGI CCWGG 1 cut(s) 172
PsyI GACNNNGTC 1 cut(s) 254
RsaI GTAC 2 cut(s) 152, 193
RsaNI GTAC 2 cut(s) 151, 192
SaqAI TTAA 3 cut(s) 213, 311, 420
SatI GCNGC 1 cut(s) 202
Sau3AI GATC 1 cut(s) 429
ScrFI CCNGG 1 cut(s) 174
SfcI CTRYAG 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 69
SmoI CTYRAG 1 cut(s) 69
Sse9I AATT 3 cut(s) 126, 371, 511
SsiI CCGC 2 cut(s) 202, 405
SspMI CTAG 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 172
TaaI ACNGT 3 cut(s) 98, 155, 483
TasI AATT 3 cut(s) 126, 371, 511
TatI WGTACW 2 cut(s) 150, 191
TauI GCSGC 1 cut(s) 204
TfiI GAWTC 2 cut(s) 20, 229
Tru1I TTAA 3 cut(s) 213, 311, 420
Tru9I TTAA 3 cut(s) 213, 311, 420
TscAI CASTG 3 cut(s) 117, 165, 486
TspRI CASTG 3 cut(s) 117, 165, 486
Tth111I GACNNNGTC 1 cut(s) 254
XbaI TCTAGA 1 cut(s) 470
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.