MD15G1162600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016721
Physical Location & Seq
Forward (+)
12249420 .. 12251766
2347 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1162600.v1.1.491

Sequence Viewer

Length: 531 bp
ATGGAGTTGTGCGCCTCTGAGAAAAAGGGCATGTGGCTTGGCTCTTTTTTGGAAGCAATGGTGGTCAACACTGTCCTTGCTGCTCCCAACTCCCTTGCTCTGCTGCTCCTTGCGGTGCTGAAGAATACTGGGGCGCTGATGAATGATATCAATACGTCACCAGAATTGACTGCAGTCACTGGAAGGTTTCCTTTTGAAGAAATTCTGAAACTGGAAAAACCTGGTGCGGAAAACAAAGATGCTAGCGATACAGAGGATGATGAAGAGGATGGAGATGATGAGGAGGTGGATGACCCAGAGGGTGATGATGCAGGAGATGAGGACCTCTCAGGTGACGAGGGGGCAGATAAAGAAGGGGATCCTGAGGATGACCCTGAAGCCAATGGTGACGGAGGCAGCGATGAAGAGGATGATGAAGATGACGACGATGATGATGGTGATGATGATGATGATGAAGATGAGGATGGAGAAGAAGAAGAGGAAGAAGAGGAGGACGAGATTTCAGAGCCACCTGCCAAAAGGAGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

177

Amino Acids

19.06

Weight (kDa)

4.05

Isoelectric Point (pI)

56.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016721)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02080
malus_domestica MD02G1018600.v1.1 MD15G1162600.v1.1
prunus_persica Prupe.7G252200_v2.0.a1 Prupe.7G252200_v2.0.a1
pyrus_communis pycom02g01670
rosa_chinensis RchiOBHm_Chr2g0087101
rosa_laevigata RLG00000015832
rosa_multiflora Rmu_ssc0000239.1_g000001
rosa_roxburghii Rroxscaffold_2G00153880 Rroxscaffold_7G00210780
rosa_rugosa Rorug01G0472500 Rorug01G0472500
rosa_wichuraiana Rw2G002050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 520
Acc36I ACCTGC 1 cut(s) 520
AciI CCGC 2 cut(s) 113, 227
AclWI GGATC 2 cut(s) 353, 366
AcsI RAATTY 1 cut(s) 201
AcuI CTGAAG 2 cut(s) 140, 396
AfiI CCNNNNNNNGG 1 cut(s) 522
AgsI TTSAA 1 cut(s) 197
AjnI CCWGG 1 cut(s) 220
AlwI GGATC 2 cut(s) 353, 366
AlwNI CAGNNNCTG 1 cut(s) 179
ApeKI GCWGC 3 cut(s) 80, 103, 396
ApoI RAATTY 1 cut(s) 201
Asp700I GAANNNNTTC 1 cut(s) 201
AspLEI GCGC 2 cut(s) 14, 136
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 5 cut(s) 150, 314, 344, 398, 449
AsuNHI GCTAGC 1 cut(s) 242
AvaII GGWCC 1 cut(s) 322
AxyI CCTNAGG 1 cut(s) 363
BamHI GGATCC 1 cut(s) 358
BbvI GCAGC 3 cut(s) 67, 90, 408
BccI CCATC 3 cut(s) 263, 428, 458
BciT130I CCWGG 1 cut(s) 222
BfaI CTAG 1 cut(s) 243
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 137
BfuAI ACCTGC 1 cut(s) 520
BisI GCNGC 3 cut(s) 81, 104, 397
BlsI GCNGC 3 cut(s) 82, 105, 398
Bme1390I CCNGG 1 cut(s) 222
Bme18I GGWCC 1 cut(s) 322
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 360
BmrFI CCNGG 1 cut(s) 222
BmrI ACTGGG 1 cut(s) 138
BmsI GCATC 2 cut(s) 229, 298
BmtI GCTAGC 1 cut(s) 246
BmuI ACTGGG 1 cut(s) 138
BoxI GACNNNNGTC 1 cut(s) 173
BplI GAGNNNNNCTC 2 cut(s) 311, 343
Bsc4I CCNNNNNNNGG 1 cut(s) 522
Bse1I ACTGG 3 cut(s) 133, 184, 216
Bse21I CCTNAGG 1 cut(s) 363
Bse3DI GCAATG 1 cut(s) 63
BseBI CCWGG 1 cut(s) 222
BseGI GGATG 6 cut(s) 262, 274, 295, 373, 415, 469
BseLI CCNNNNNNNGG 1 cut(s) 522
BseMI GCAATG 1 cut(s) 63
BseMII CTCAG 3 cut(s) 9, 342, 354
BseNI ACTGG 3 cut(s) 133, 184, 216
BseRI GAGGAG 2 cut(s) 296, 503
BseXI GCAGC 3 cut(s) 67, 90, 408
BslI CCNNNNNNNGG 1 cut(s) 522
Bsp143I GATC 1 cut(s) 358
BspACI CCGC 2 cut(s) 113, 227
BspCNI CTCAG 3 cut(s) 10, 341, 355
BspLI GGNNCC 1 cut(s) 360
BspMAI CTGCAG 1 cut(s) 175
BspMI ACCTGC 1 cut(s) 520
BspOI GCTAGC 1 cut(s) 246
BspPI GGATC 2 cut(s) 353, 366
BsrDI GCAATG 1 cut(s) 63
BsrI ACTGG 3 cut(s) 133, 184, 216
BssMI GATC 1 cut(s) 358
Bst2UI CCWGG 1 cut(s) 222
Bst4CI ACNGT 1 cut(s) 73
Bst6I CTCTTC 4 cut(s) 258, 399, 471, 480
BstC8I GCNNGC 1 cut(s) 244
BstDEI CTNAG 3 cut(s) 18, 328, 363
BstF5I GGATG 6 cut(s) 262, 274, 295, 373, 415, 469
BstH2I RGCGCY 1 cut(s) 137
BstHHI GCGC 2 cut(s) 14, 136
BstKTI GATC 1 cut(s) 361
BstMBI GATC 1 cut(s) 358
BstNI CCWGG 1 cut(s) 222
BstNSI RCATGY 1 cut(s) 34
BstPAI GACNNNNGTC 1 cut(s) 173
BstSCI CCNGG 1 cut(s) 220
BstSFI CTRYAG 1 cut(s) 171
BstV1I GCAGC 3 cut(s) 67, 90, 408
BstX2I RGATCY 1 cut(s) 358
BstYI RGATCY 1 cut(s) 358
Bsu36I CCTNAGG 1 cut(s) 363
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 6 cut(s) 262, 274, 295, 373, 415, 469
BtsIMutI CAGTG 2 cut(s) 69, 177
BveI ACCTGC 1 cut(s) 520
Cac8I GCNNGC 1 cut(s) 244
CaiI CAGNNNCTG 1 cut(s) 179
CfoI GCGC 2 cut(s) 14, 136
Cfr13I GGNCC 1 cut(s) 322
CsiI ACCWGGT 1 cut(s) 220
CviAII CATG 1 cut(s) 31
CviJI RGCY 4 cut(s) 37, 42, 380, 508
CviKI_1 RGCY 4 cut(s) 37, 42, 380, 508
DdeI CTNAG 3 cut(s) 18, 328, 363
DpnI GATC 1 cut(s) 360
DpnII GATC 1 cut(s) 358
Eam1104I CTCTTC 4 cut(s) 258, 399, 471, 480
EarI CTCTTC 4 cut(s) 258, 399, 471, 480
Eco32I GATATC 1 cut(s) 148
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 2 cut(s) 140, 396
Eco81I CCTNAGG 1 cut(s) 363
EcoO109I RGGNCCY 1 cut(s) 322
EcoRII CCWGG 1 cut(s) 220
EcoRV GATATC 1 cut(s) 148
FaeI CATG 1 cut(s) 34
FaiI YATR 1 cut(s) 32
FalI AAGNNNNNCTT 2 cut(s) 175, 207
FatI CATG 1 cut(s) 30
Fnu4HI GCNGC 3 cut(s) 81, 104, 397
FokI GGATG 6 cut(s) 269, 281, 302, 380, 422, 476
Fsp4HI GCNGC 3 cut(s) 81, 104, 397
FspBI CTAG 1 cut(s) 243
GlaI GCGC 2 cut(s) 13, 135
GluI GCNGC 3 cut(s) 81, 104, 397
HaeII RGCGCY 1 cut(s) 137
HhaI GCGC 2 cut(s) 14, 136
Hin1II CATG 1 cut(s) 34
Hin6I GCGC 2 cut(s) 12, 134
HinP1I GCGC 2 cut(s) 12, 134
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HphI GGTGA 5 cut(s) 150, 314, 344, 398, 449
Hpy166II GTNNAC 1 cut(s) 67
Hpy188I TCNGA 3 cut(s) 19, 207, 505
Hpy188III TCNNGA 1 cut(s) 362
Hpy8I GTNNAC 1 cut(s) 67
Hpy99I CGWCG 1 cut(s) 428
HpyAV CCTTC 2 cut(s) 177, 347
HpyCH4III ACNGT 1 cut(s) 73
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 2 cut(s) 173, 311
HpyF3I CTNAG 3 cut(s) 18, 328, 363
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 1 cut(s) 34
HspAI GCGC 2 cut(s) 12, 134
Kzo9I GATC 1 cut(s) 358
LmnI GCTCC 2 cut(s) 88, 111
Lsp1109I GCAGC 3 cut(s) 67, 90, 408
LweI GCATC 2 cut(s) 229, 298
MabI ACCWGGT 1 cut(s) 220
MaeI CTAG 1 cut(s) 243
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 4 cut(s) 156, 175, 332, 386
MalI GATC 1 cut(s) 360
MboI GATC 1 cut(s) 358
MflI RGATCY 1 cut(s) 358
MluCI AATT 2 cut(s) 164, 201
MroXI GAANNNNTTC 1 cut(s) 201
MspR9I CCNGG 1 cut(s) 222
MvaI CCWGG 1 cut(s) 222
NdeII GATC 1 cut(s) 358
NheI GCTAGC 1 cut(s) 242
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 360
NmuCI GTSAC 4 cut(s) 156, 175, 332, 386
NspI RCATGY 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 520
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PdmI GAANNNNTTC 1 cut(s) 201
PkrI GCNGC 3 cut(s) 82, 105, 398
PpuMI RGGWCCY 1 cut(s) 322
PshAI GACNNNNGTC 1 cut(s) 173
Psp5II RGGWCCY 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 220
PspGI CCWGG 1 cut(s) 220
PspN4I GGNNCC 1 cut(s) 360
PspPI GGNCC 1 cut(s) 322
PspPPI RGGWCCY 1 cut(s) 322
PstI CTGCAG 1 cut(s) 175
PstNI CAGNNNCTG 1 cut(s) 179
PsuI RGATCY 1 cut(s) 358
SatI GCNGC 3 cut(s) 81, 104, 397
Sau3AI GATC 1 cut(s) 358
Sau96I GGNCC 1 cut(s) 322
ScrFI CCNGG 1 cut(s) 222
SetI ASST 7 cut(s) 158, 188, 223, 288, 327, 334, 514
SexAI ACCWGGT 1 cut(s) 220
SfaNI GCATC 2 cut(s) 229, 298
SfcI CTRYAG 1 cut(s) 171
SinI GGWCC 1 cut(s) 322
Sse9I AATT 2 cut(s) 164, 201
SsiI CCGC 2 cut(s) 113, 227
SspMI CTAG 1 cut(s) 243
StyD4I CCNGG 1 cut(s) 220
TaaI ACNGT 1 cut(s) 73
TaiI ACGT 1 cut(s) 158
TasI AATT 2 cut(s) 164, 201
TscAI CASTG 2 cut(s) 76, 184
TseFI GTSAC 4 cut(s) 156, 175, 332, 386
TseI GCWGC 3 cut(s) 80, 103, 396
Tsp45I GTSAC 4 cut(s) 156, 175, 332, 386
TspDTI ATGAA 5 cut(s) 155, 276, 417, 429, 468
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 2 cut(s) 76, 184
VpaK11BI GGWCC 1 cut(s) 322
XapI RAATTY 1 cut(s) 201
XceI RCATGY 1 cut(s) 34
XmnI GAANNNNTTC 1 cut(s) 201
XspI CTAG 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.