Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0009378

Physical Location & Seq

Genomic Coordinates Forward (+)

13877192 .. 13880754

Gene Structure 3563 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1177300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1149 bp

ATGGGTAGCTACAAAAACGGCGTTGTCGGGGACCAAAGCGGCACCAGCAATGGCGACGCTGGTGGCGCCGCGGTGGACAAAGGCGTCGACTTCGCCAACTACTTCTGCACCTACGCCTTCCTCTACCACCAGAAGGAGATGCTCTCTGATCGAGTCCGCATGGACGCTTACTACAACGCCGTTTTCAAGAACACGCACCACTTCCATGGCAAGGCTGTGTTGGATGTGGGAGCAGGAAGTGGCATTCTCGCAATTTGGTCAGCGCAAGCCGGGGCGAGGAAGGTTTACGCCGTTGAAGCGACTTCCATGGCTGAACATGCACGTGCAGTTGTGAAAGCGAATAATCTTCAAGACGTGGTTGAGGTGATTGAGGGCTCCATGGAGGATGTTGTCCTGCCAGAGAAAGTGGATGTGATAATTTCGGAGTGGATGGGGTATTTCCTACTGCGTGAATCAATGTTTGATTCTGTGATATGCGCACGTGACCGATGGTTAAAGCCAACTGGAGTCATGTATCCTAGCCATGCTACTATGTGGGTGGCACCTATTAGGACTGGATTGGGAGATCAAAAAAGTAATGATTATGAGTCTTCTATGGACGATTGGGAACGTTTCACAGATGAGACCAAAAATTATTATGGTGTTGATATGAGTGTTTTAACAAAGCCTTTCTCTCAAGAGCAGAAGAAATACTATCTTCAGACATCATTGTGGAACAACCTTCATCCGCATCAAGTTATAGGTGCAGCTGCTTCAATAAAGGAGATTGATTGTTTAACTGCCTCAGTCAGTGACATCCTTGAAGTCAAATCAAACTTTTCGTCAACAGTAACTCTTCAGAACACAAGGCTTTGTGGCTACGGGGGATGGTTTGATGTTCAATTTCGAGGAAGAAAGGAGGATCCAGCTCAGGAAGAAGTTGAGTTGACTACCGCTCCTAGTGTGAACGATGGCACTCACTGGGGCCAACAGGTTTTTCTGTTGAGCCCTCCTGCTCGCATCACTGAAGGGGATCAACTAAATGGTTCTTTCTCAATGAAACGATCAAAGGAGAATCATAGATTGATGGAGGCTGAGTTCAGCTGTGAGATTAGACAGCAATCTGGCGAGTTAGTTCCTCGTTTCACAAATAGATATTTCATAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006325 GO:0006355 GO:0006464 GO:0006479 GO:0006807 GO:0006996 GO:0007275 GO:0008150 GO:0008152 GO:0008168 GO:0008170 GO:0008213 GO:0008276 GO:0008469 GO:0008757 GO:0009791 GO:0009889 GO:0009987 GO:0010228 GO:0010468 GO:0010556 GO:0016043 GO:0016273 GO:0016274 GO:0016277 GO:0016569 GO:0016570 GO:0016571 GO:0016740 GO:0016741 GO:0018193 GO:0018195 GO:0018216 GO:0019219 GO:0019222 GO:0019538 GO:0019919 GO:0022414 GO:0031323 GO:0031326 GO:0032259 GO:0032501 GO:0032502 GO:0034969 GO:0035241 GO:0035242 GO:0035246 GO:0035247 GO:0036211 GO:0042054 GO:0043170 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0051276 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0071840 GO:0080090 GO:0140096 GO:1901564 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko04068 ko04922 map04068 map04922

Pfam Domains

Protein Families

PrmA

Protein Analysis

383

Amino Acids

42.84

Weight (kDa)

5.3

Isoelectric Point (pI)

35.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTS	PF05175	67 - 141	7.5e-08	Methyltransferase small domain
PrmA	PF06325	69 - 141	1.4e-10	Ribosomal protein L11 methyltransferase (PrmA)
TRM5-TYW2_MTfase	PF02475	69 - 144	8.6e-06	TRM5/TYW2 methyltransferase domain
Methyltransf_25	PF13649	73 - 169	1.6e-09	Methyltransferase domain
Methyltransf_11	PF08241	74 - 171	2.2e-08	Methyltransferase domain
PRMT_C	PF22528	176 - 364	9e-23	Arginine methyltransferase oligomerization subdomain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0009378)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G04870
fragaria_vesca	FvH4_1g03880 FvH4_1g03880 FvH4_1g03880 FvH4_1g03880
malus_domestica	MD02G1037100.v1.1 MD15G1177300.v1.1
prunus_persica	Prupe.7G238300_v2.0.a1
pyrus_communis	pycom02g03040 pycom15g15860
rosa_chinensis	RchiOBHm_Chr2g0089261 RchiOBHm_Chr2g0089341
rosa_laevigata	RLG00000016009
rosa_multiflora	Rmu_sc0003382.1_g000036 Rmu_sc0008322.1_g000018
rosa_roxburghii	Rroxscaffold_2G00152080
rosa_rugosa	Rorug01G0488700
rosa_samantha	Rh2AG044900 Rh2BG043400 Rh2CG044800 Rh2DG043700
rosa_wichuraiana	Rw2G003850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	83
Acc16I	TGCGCA	1 cut(s)	478
AccB1I	GGYRCC	3 cut(s)	41, 65, 541
AccBSI	CCGCTC	1 cut(s)	935
AccI	GTMKAC	1 cut(s)	87
AccII	CGCG	1 cut(s)	71
AciI	CCGC	6 cut(s)	39, 69, 71, 157, 728, 933
AclI	AACGTT	1 cut(s)	610
AclWI	GGATC	3 cut(s)	896, 909, 1020
AcuI	CTGAAG	3 cut(s)	683, 821, 1026
AcvI	CACGTG	2 cut(s)	323, 482
AcyI	GRCGYC	2 cut(s)	66, 84
AfiI	CCNNNNNNNGG	3 cut(s)	133, 211, 276
AgsI	TTSAA	6 cut(s)	187, 296, 350, 756, 803, 881
AjiI	CACGTC	1 cut(s)	355
AjuI	GAANNNNNNNTTGG	2 cut(s)	960, 992
AloI	GAACNNNNNNTCC	2 cut(s)	1061, 1093
AluBI	AGCT	4 cut(s)	9, 749, 908, 1083
AluI	AGCT	4 cut(s)	9, 749, 908, 1083
Alw26I	GTCTC	1 cut(s)	617
AlwI	GGATC	3 cut(s)	896, 909, 1020
AoxI	GGCC	1 cut(s)	964
ApeKI	GCWGC	2 cut(s)	746, 749
ArsI	GACNNNNNNTTYG	2 cut(s)	806, 838
AspLEI	GCGC	3 cut(s)	68, 265, 479
AspS9I	GGNCC	2 cut(s)	31, 964
AsuC2I	CCSGG	1 cut(s)	271
AsuHPI	GGTGA	1 cut(s)	376
AvaII	GGWCC	1 cut(s)	31
BamHI	GGATCC	1 cut(s)	901
BanI	GGYRCC	3 cut(s)	41, 65, 541
BanII	GRGCYC	2 cut(s)	377, 989
BbrPI	CACGTG	2 cut(s)	323, 482
BbsI	GAAGAC	1 cut(s)	582
BbvI	GCAGC	2 cut(s)	736, 758
BccI	CCATC	5 cut(s)	424, 483, 861, 944, 1060
BceAI	ACGGC	3 cut(s)	34, 164, 275
BciVI	GTATCC	1 cut(s)	525
BcnI	CCSGG	1 cut(s)	271
BcoDI	GTCTC	1 cut(s)	617
BfaI	CTAG	2 cut(s)	519, 939
BfoI	RGCGCY	1 cut(s)	69
BfuI	GTATCC	1 cut(s)	525
BisI	GCNGC	4 cut(s)	40, 69, 747, 750
BlsI	GCNGC	4 cut(s)	41, 70, 748, 751
Bme1390I	CCNGG	1 cut(s)	271
Bme18I	GGWCC	1 cut(s)	31
BmgBI	CACGTC	1 cut(s)	355
BmgT120I	GGNCC	2 cut(s)	31, 964
BmiI	GGNNCC	7 cut(s)	32, 43, 67, 376, 543, 903, 965
BmrFI	CCNGG	1 cut(s)	271
BmrI	ACTGGG	1 cut(s)	970
BmsI	GCATC	3 cut(s)	129, 739, 1008
BmuI	ACTGGG	1 cut(s)	970
BpiI	GAAGAC	1 cut(s)	582
BplI	GAGNNNNNCTC	2 cut(s)	128, 160
BpmI	CTGGAG	1 cut(s)	525
Bpu10I	CCTNAGC	1 cut(s)	909
BpuEI	CTTGAG	1 cut(s)	660
BpuMI	CCSGG	1 cut(s)	271
BsaAI	YACGTR	2 cut(s)	323, 482
BsaHI	GRCGYC	2 cut(s)	66, 84
BsaI	GGTCTC	1 cut(s)	617
BsaJI	CCNNGG	5 cut(s)	69, 205, 270, 306, 378
BsaXI	ACNNNNNCTCC	4 cut(s)	919, 949, 1061, 1091
Bsc4I	CCNNNNNNNGG	3 cut(s)	133, 211, 276
Bse1I	ACTGG	3 cut(s)	508, 559, 965
Bse3DI	GCAATG	1 cut(s)	55
BseDI	CCNNGG	5 cut(s)	69, 205, 270, 306, 378
BseGI	GGATG	7 cut(s)	229, 391, 415, 435, 724, 795, 872
BseLI	CCNNNNNNNGG	3 cut(s)	133, 211, 276
BseMI	GCAATG	1 cut(s)	55
BseMII	CTCAG	3 cut(s)	798, 923, 1065
BseNI	ACTGG	3 cut(s)	508, 559, 965
BseXI	GCAGC	2 cut(s)	736, 758
BsgI	GTGCAG	3 cut(s)	91, 345, 765
Bsh1236I	CGCG	1 cut(s)	71
BshFI	GGCC	1 cut(s)	966
BshNI	GGYRCC	3 cut(s)	41, 65, 541
BsiSI	CCGG	1 cut(s)	270
BslFI	GGGAC	1 cut(s)	44
BslI	CCNNNNNNNGG	3 cut(s)	133, 211, 276
BsmAI	GTCTC	1 cut(s)	617
BsmFI	GGGAC	1 cut(s)	44
BsmI	GAATGC	1 cut(s)	243
BsnI	GGCC	1 cut(s)	966
Bso31I	GGTCTC	1 cut(s)	617
Bsp1286I	GDGCHC	2 cut(s)	377, 989
Bsp143I	GATC	5 cut(s)	148, 565, 901, 1012, 1043
Bsp19I	CCATGG	3 cut(s)	205, 306, 378
BspACI	CCGC	6 cut(s)	39, 69, 71, 157, 728, 933
BspANI	GGCC	1 cut(s)	966
BspCNI	CTCAG	3 cut(s)	797, 922, 1066
BspFNI	CGCG	1 cut(s)	71
BspLI	GGNNCC	7 cut(s)	32, 43, 67, 376, 543, 903, 965
BspPI	GGATC	3 cut(s)	896, 909, 1020
BspT107I	GGYRCC	3 cut(s)	41, 65, 541
BspTNI	GGTCTC	1 cut(s)	617
BsrBI	CCGCTC	1 cut(s)	935
BsrDI	GCAATG	1 cut(s)	55
BsrI	ACTGG	3 cut(s)	508, 559, 965
BssECI	CCNNGG	5 cut(s)	69, 205, 270, 306, 378
BssMI	GATC	5 cut(s)	148, 565, 901, 1012, 1043
BssNI	GRCGYC	2 cut(s)	66, 84
BssT1I	CCWWGG	3 cut(s)	205, 306, 378
Bst4CI	ACNGT	1 cut(s)	829
Bst6I	CTCTTC	1 cut(s)	840
BstACI	GRCGYC	2 cut(s)	66, 84
BstBAI	YACGTR	2 cut(s)	323, 482
BstC8I	GCNNGC	2 cut(s)	267, 997
BstDEI	CTNAG	3 cut(s)	784, 909, 1074
BstDSI	CCRYGG	4 cut(s)	69, 205, 306, 378
BstF5I	GGATG	7 cut(s)	229, 391, 415, 435, 724, 795, 872
BstFNI	CGCG	1 cut(s)	71
BstH2I	RGCGCY	1 cut(s)	69
BstHHI	GCGC	3 cut(s)	68, 265, 479
BstKTI	GATC	5 cut(s)	151, 568, 904, 1015, 1046
BstMAI	GTCTC	1 cut(s)	617
BstMBI	GATC	5 cut(s)	148, 565, 901, 1012, 1043
BstMWI	GCNNNNNNNGC	4 cut(s)	45, 65, 296, 317
BstNSI	RCATGY	1 cut(s)	320
BstSCI	CCNGG	1 cut(s)	269
BstUI	CGCG	1 cut(s)	71
BstV1I	GCAGC	2 cut(s)	736, 758
BstV2I	GAAGAC	1 cut(s)	582
BstX2I	RGATCY	1 cut(s)	901
BstXI	CCANNNNNNTGG	1 cut(s)	206
BstYI	RGATCY	1 cut(s)	901
BsuI	GTATCC	1 cut(s)	525
BsuRI	GGCC	1 cut(s)	966
BtgI	CCRYGG	4 cut(s)	69, 205, 306, 378
BtrI	CACGTC	1 cut(s)	355
BtsCI	GGATG	7 cut(s)	229, 391, 415, 435, 724, 795, 872
BtsIMutI	CAGTG	3 cut(s)	796, 958, 1002
Cac8I	GCNNGC	2 cut(s)	267, 997
CfoI	GCGC	3 cut(s)	68, 265, 479
Cfr13I	GGNCC	2 cut(s)	31, 964
Cfr42I	CCGCGG	1 cut(s)	72
CseI	GACGC	3 cut(s)	65, 73, 173
CviAII	CATG	7 cut(s)	160, 206, 307, 317, 379, 511, 524
DdeI	CTNAG	3 cut(s)	784, 909, 1074
DinI	GGCGCC	1 cut(s)	67
DpnI	GATC	5 cut(s)	150, 567, 903, 1014, 1045
DpnII	GATC	5 cut(s)	148, 565, 901, 1012, 1043
DrdI	GACNNNNNNGTC	1 cut(s)	83
DseDI	GACNNNNNNGTC	1 cut(s)	83
Eam1104I	CTCTTC	1 cut(s)	840
EarI	CTCTTC	1 cut(s)	840
Eco130I	CCWWGG	3 cut(s)	205, 306, 378
Eco24I	GRGCYC	2 cut(s)	377, 989
Eco31I	GGTCTC	1 cut(s)	617
Eco47I	GGWCC	1 cut(s)	31
Eco57I	CTGAAG	3 cut(s)	683, 821, 1026
Eco72I	CACGTG	2 cut(s)	323, 482
EcoT14I	CCWWGG	3 cut(s)	205, 306, 378
EcoT38I	GRGCYC	2 cut(s)	377, 989
EgeI	GGCGCC	1 cut(s)	67
EheI	GGCGCC	1 cut(s)	67
ErhI	CCWWGG	3 cut(s)	205, 306, 378
FaeI	CATG	7 cut(s)	163, 209, 310, 320, 382, 514, 527
FaqI	GGGAC	1 cut(s)	44
FatI	CATG	7 cut(s)	159, 205, 306, 316, 378, 510, 523
FblI	GTMKAC	1 cut(s)	87
Fnu4HI	GCNGC	4 cut(s)	40, 69, 747, 750
FokI	GGATG	7 cut(s)	236, 398, 422, 442, 711, 782, 879
FriOI	GRGCYC	2 cut(s)	377, 989
Fsp4HI	GCNGC	4 cut(s)	40, 69, 747, 750
FspAI	RTGCGCAY	1 cut(s)	478
FspBI	CTAG	2 cut(s)	519, 939
FspI	TGCGCA	1 cut(s)	478
GlaI	GCGC	3 cut(s)	67, 264, 478
GluI	GCNGC	4 cut(s)	40, 69, 747, 750
GsuI	CTGGAG	1 cut(s)	525
HaeII	RGCGCY	1 cut(s)	69
HaeIII	GGCC	1 cut(s)	966
HapII	CCGG	1 cut(s)	270
HgaI	GACGC	3 cut(s)	65, 73, 173
HhaI	GCGC	3 cut(s)	68, 265, 479
Hin1I	GRCGYC	2 cut(s)	66, 84
Hin1II	CATG	7 cut(s)	163, 209, 310, 320, 382, 514, 527
Hin6I	GCGC	3 cut(s)	66, 263, 477
HinP1I	GCGC	3 cut(s)	66, 263, 477
HincII	GTYRAC	3 cut(s)	88, 825, 927
HindII	GTYRAC	3 cut(s)	88, 825, 927
HinfI	GANTC	6 cut(s)	153, 452, 464, 507, 587, 1054
HpaII	CCGG	1 cut(s)	270
HphI	GGTGA	1 cut(s)	376
Hpy166II	GTNNAC	6 cut(s)	76, 88, 286, 825, 927, 946
Hpy188I	TCNGA	4 cut(s)	148, 424, 702, 840
Hpy188III	TCNNGA	4 cut(s)	187, 350, 677, 911
Hpy8I	GTNNAC	6 cut(s)	76, 88, 286, 825, 927, 946
Hpy99I	CGWCG	2 cut(s)	59, 89
HpyAV	CCTTC	5 cut(s)	127, 127, 274, 731, 1001
HpyCH4III	ACNGT	1 cut(s)	829
HpyCH4IV	ACGT	4 cut(s)	322, 354, 481, 610
HpyCH4V	TGCA	4 cut(s)	108, 320, 326, 746
HpyF10VI	GCNNNNNNNGC	4 cut(s)	45, 65, 296, 317
HpyF3I	CTNAG	3 cut(s)	784, 909, 1074
HpySE526I	ACGT	4 cut(s)	322, 354, 481, 610
Hsp92I	GRCGYC	2 cut(s)	66, 84
Hsp92II	CATG	7 cut(s)	163, 209, 310, 320, 382, 514, 527
HspAI	GCGC	3 cut(s)	66, 263, 477
KasI	GGCGCC	1 cut(s)	65
KspI	CCGCGG	1 cut(s)	72
Kzo9I	GATC	5 cut(s)	148, 565, 901, 1012, 1043
LmnI	GCTCC	3 cut(s)	230, 380, 940
Lsp1109I	GCAGC	2 cut(s)	736, 758
LweI	GCATC	3 cut(s)	129, 739, 1008
MaeI	CTAG	2 cut(s)	519, 939
MaeII	ACGT	4 cut(s)	322, 354, 481, 610
MaeIII	GTNAC	3 cut(s)	482, 791, 829
MalI	GATC	5 cut(s)	150, 567, 903, 1014, 1045
MbiI	CCGCTC	1 cut(s)	935
MboI	GATC	5 cut(s)	148, 565, 901, 1012, 1043
MboII	GAAGA	7 cut(s)	338, 582, 689, 697, 827, 903, 926
MflI	RGATCY	1 cut(s)	901
MhlI	GDGCHC	2 cut(s)	377, 989
MluCI	AATT	4 cut(s)	252, 417, 631, 881
Mly113I	GGCGCC	1 cut(s)	66
MlyI	GAGTC	3 cut(s)	162, 516, 596
MmeI	TCCRAC	1 cut(s)	201
MseI	TTAA	3 cut(s)	494, 659, 776
MslI	CAYNNNNRTG	3 cut(s)	204, 321, 709
MspA1I	CMGCKG	3 cut(s)	71, 749, 1083
MspI	CCGG	1 cut(s)	270
MspR9I	CCNGG	1 cut(s)	271
Mva1269I	GAATGC	1 cut(s)	243
MvnI	CGCG	1 cut(s)	71
MwoI	GCNNNNNNNGC	4 cut(s)	45, 65, 296, 317
NarI	GGCGCC	1 cut(s)	66
NciI	CCSGG	1 cut(s)	271
NcoI	CCATGG	3 cut(s)	205, 306, 378
NdeII	GATC	5 cut(s)	148, 565, 901, 1012, 1043
NlaIII	CATG	7 cut(s)	163, 209, 310, 320, 382, 514, 527
NlaIV	GGNNCC	7 cut(s)	32, 43, 67, 376, 543, 903, 965
NmuCI	GTSAC	2 cut(s)	482, 791
NsbI	TGCGCA	1 cut(s)	478
NspI	RCATGY	1 cut(s)	320
PctI	GAATGC	1 cut(s)	243
PfeI	GAWTC	3 cut(s)	452, 464, 1054
PkrI	GCNGC	4 cut(s)	41, 70, 748, 751
PleI	GAGTC	3 cut(s)	161, 515, 595
PluTI	GGCGCC	1 cut(s)	69
PmaCI	CACGTG	2 cut(s)	323, 482
PmlI	CACGTG	2 cut(s)	323, 482
PpsI	GAGTC	3 cut(s)	161, 515, 595
Ppu21I	YACGTR	2 cut(s)	323, 482
Psp1406I	AACGTT	1 cut(s)	610
PspCI	CACGTG	2 cut(s)	323, 482
PspN4I	GGNNCC	7 cut(s)	32, 43, 67, 376, 543, 903, 965
PspPI	GGNCC	2 cut(s)	31, 964
PsuI	RGATCY	1 cut(s)	901
PvuII	CAGCTG	2 cut(s)	749, 1083
RseI	CAYNNNNRTG	3 cut(s)	204, 321, 709
SacII	CCGCGG	1 cut(s)	72
SalI	GTCGAC	1 cut(s)	86
SaqAI	TTAA	3 cut(s)	494, 659, 776
SatI	GCNGC	4 cut(s)	40, 69, 747, 750
Sau3AI	GATC	5 cut(s)	148, 565, 901, 1012, 1043
Sau96I	GGNCC	2 cut(s)	31, 964
SchI	GAGTC	3 cut(s)	162, 516, 596
ScrFI	CCNGG	1 cut(s)	271
SduI	GDGCHC	2 cut(s)	377, 989
SfaNI	GCATC	3 cut(s)	129, 739, 1008
SfoI	GGCGCC	1 cut(s)	67
Sfr303I	CCGCGG	1 cut(s)	72
SgrBI	CCGCGG	1 cut(s)	72
SinI	GGWCC	1 cut(s)	31
SmiMI	CAYNNNNRTG	3 cut(s)	204, 321, 709
SmlI	CTYRAG	1 cut(s)	675
SmoI	CTYRAG	1 cut(s)	675
Sse9I	AATT	4 cut(s)	252, 417, 631, 881
SsiI	CCGC	6 cut(s)	39, 69, 71, 157, 728, 933
SspDI	GGCGCC	1 cut(s)	65
SspMI	CTAG	2 cut(s)	519, 939
StyD4I	CCNGG	1 cut(s)	269
StyI	CCWWGG	3 cut(s)	205, 306, 378
TaaI	ACNGT	1 cut(s)	829
TaiI	ACGT	4 cut(s)	325, 357, 484, 613
TaqI	TCGA	3 cut(s)	87, 151, 886
TaqII	GACCGA	1 cut(s)	501
TasI	AATT	4 cut(s)	252, 417, 631, 881
TauI	GCSGC	2 cut(s)	42, 71
TfiI	GAWTC	3 cut(s)	452, 464, 1054
Tru1I	TTAA	3 cut(s)	494, 659, 776
Tru9I	TTAA	3 cut(s)	494, 659, 776
TscAI	CASTG	3 cut(s)	796, 965, 1009
TseFI	GTSAC	2 cut(s)	482, 791
TseI	GCWGC	2 cut(s)	746, 749
Tsp45I	GTSAC	2 cut(s)	482, 791
TspDTI	ATGAA	3 cut(s)	713, 1052, 1129
TspRI	CASTG	3 cut(s)	796, 965, 1009
VpaK11BI	GGWCC	1 cut(s)	31
XceI	RCATGY	1 cut(s)	320
XmiI	GTMKAC	1 cut(s)	87
XspI	CTAG	2 cut(s)	519, 939

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.