Rh2BG043400
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N- methyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0009378
Physical Location & Seq
Forward (+)
2992660 .. 2996533
3874 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG043400.1

Sequence Viewer

Length: 1113 bp
ATGGGGAGCTGCACAAACGGCGTCGTTTCAGACCAAAGCGGCGCCACCAGCAATGGCCGCGCGTCGGTGGACAAAGGCGTCGACTTTGCCAATTACTTCTGCACCTACGCCTTCCTCTACCACCAGAAGGAGATGCTCTCCGATCGTGTTCGCATGGACGCATACTACAACGCCGTCTTCCAGAACAAGCACCACTTCAATGGCAAGGTTGTGTTGGATGTGGGAACTGGGAGTGGCATTCTCGCAATTTGGTCTGCGCAAGCTGGTGCTAGGAAGGTGTATGCTGTGGAAGCCACTAACATGGCTGAACATGCGCGTGAGCTTGTGAAAGCGAATAATGTGCAAGACGTGGTTGAGGTGATTCAGGGCTCTATGGAGGATGTTCAGCTTCCAGAGAAAGTTGATGTGATAATTTCGGAGTGGATGGGGTATTTCCTACTGCGTGAGTCGATGTTTGACTCTGTGATATGTGCCCGTGACCGATGGTTGAAGCCAACTGGAGTCATGTATCCTAGTCATGCTCGTATGTGGGTGGCACCTATTAGGTCTGGGTTAGGAGATCAAAAACAAAATGATTATGAATCATCGATGGAAGACTGGTATCGTTTCACAGATGAAACACAATCTTATTATGGTGTTGATATGGGTGCTCTAACAAAACCTTTCGCTGAAGAGCAGAAGAAATATTATCTTCAGACATCATTGTGGAACAACCTTCATCCCAACCAAGTTATAGGGACAGCTGCTATACTAAGAGAGATTGATTGTTTAACTGCCTCTGTCAGTGACATCCTCGAAGTGAGATCAAACTTTTTATCTACAGTAACTCTTCCGAACACAAGGCTCTGTGGGTTCGCAGGATGGTTTGATGTTCATTTTCGAGGACGCAAGGAGGTTCCAGCCCAGAATGAAATTGAGTTGACCACTGCTCCCAGTGTAGATAATGGCACACACTGGGGCCAACAGGTTTGTTATACCCAAATCCTTTGTCCTATGTTTTTATTTATCGTCGAGAAATCAATTTGCAGATGGAAAATATTTTGTGCAAAATTCAAAAGAGATCTTCAACATTATCGTAAACTCTGTATCACTGCAGTCAAATGCTTATATTAG

Protein Analysis

370

Amino Acids

42.08

Weight (kDa)

6.32

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRMT5 PF05185 62 - 147 4.1e-06 PRMT5 arginine-N-methyltransferase
PrmA PF06325 67 - 140 5.6e-12 Ribosomal protein L11 methyltransferase (PrmA)
MTS PF05175 67 - 139 4.2e-07 Methyltransferase small domain
Methyltransf_31 PF13847 67 - 175 6.9e-07 Methyltransferase domain
TRM5-TYW2_MTfase PF02475 67 - 142 8.2e-07 TRM5/TYW2 methyltransferase domain
Methyltransf_25 PF13649 71 - 167 5.5e-11 Methyltransferase domain
Methyltransf_11 PF08241 72 - 169 4.8e-10 Methyltransferase domain
PRMT_C PF22528 190 - 327 1.3e-12 Arginine methyltransferase oligomerization subdomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 77
Acc16I TGCGCA 1 cut(s) 258
AccB1I GGYRCC 2 cut(s) 41, 535
AccI GTMKAC 1 cut(s) 81
AccII CGCG 3 cut(s) 60, 62, 316
AciI CCGC 2 cut(s) 39, 58
AcoI YGGCCR 1 cut(s) 55
AcsI RAATTY 1 cut(s) 1049
AcuI CTGAAG 2 cut(s) 677, 690
AcyI GRCGYC 3 cut(s) 21, 42, 78
AfiI CCNNNNNNNGG 2 cut(s) 64, 127
AgsI TTSAA 4 cut(s) 199, 490, 1054, 1067
AjiI CACGTC 1 cut(s) 349
AluBI AGCT 5 cut(s) 9, 263, 322, 388, 743
AluI AGCT 5 cut(s) 9, 263, 322, 388, 743
Alw21I GWGCWC 1 cut(s) 652
AoxI GGCC 2 cut(s) 55, 958
ApeKI GCWGC 2 cut(s) 9, 743
ApoI RAATTY 1 cut(s) 1049
ArsI GACNNNNNNTTYG 2 cut(s) 973, 1005
AspLEI GCGC 4 cut(s) 44, 62, 259, 316
AspS9I GGNCC 1 cut(s) 958
AsuHPI GGTGA 1 cut(s) 370
BaeGI GKGCMC 1 cut(s) 475
BanI GGYRCC 2 cut(s) 41, 535
BanII GRGCYC 1 cut(s) 371
BbsI GAAGAC 2 cut(s) 169, 600
Bbv12I GWGCWC 1 cut(s) 652
BbvI GCAGC 1 cut(s) 730
BccI CCATC 5 cut(s) 418, 477, 583, 855, 1023
BceAI ACGGC 2 cut(s) 34, 158
BcgI CGANNNNNNTGC 2 cut(s) 322, 356
BciVI GTATCC 1 cut(s) 519
BfaI CTAG 2 cut(s) 270, 513
BfmI CTRYAG 2 cut(s) 819, 1092
BfoI RGCGCY 1 cut(s) 45
BfuI GTATCC 1 cut(s) 519
BglII AGATCT 1 cut(s) 1060
BisI GCNGC 4 cut(s) 10, 40, 58, 744
BlsI GCNGC 4 cut(s) 11, 41, 59, 745
BmgBI CACGTC 1 cut(s) 349
BmgT120I GGNCC 1 cut(s) 958
BmiI GGNNCC 4 cut(s) 43, 537, 897, 959
BmrI ACTGGG 3 cut(s) 237, 927, 964
BmsI GCATC 1 cut(s) 123
BmuI ACTGGG 3 cut(s) 237, 927, 964
BpiI GAAGAC 2 cut(s) 169, 600
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpmI CTGGAG 1 cut(s) 519
Bsa29I ATCGAT 1 cut(s) 587
BsaHI GRCGYC 3 cut(s) 21, 42, 78
BsaXI ACNNNNNCTCC 2 cut(s) 913, 943
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 127
Bse1I ACTGG 5 cut(s) 232, 502, 602, 933, 959
Bse3DI GCAATG 1 cut(s) 58
BseCI ATCGAT 1 cut(s) 587
BseGI GGATG 6 cut(s) 223, 385, 429, 718, 789, 866
BseLI CCNNNNNNNGG 2 cut(s) 64, 127
BseMI GCAATG 1 cut(s) 58
BseNI ACTGG 5 cut(s) 232, 502, 602, 933, 959
BseSI GKGCMC 1 cut(s) 475
BseXI GCAGC 1 cut(s) 730
BsgI GTGCAG 1 cut(s) 85
Bsh1236I CGCG 3 cut(s) 60, 62, 316
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 2 cut(s) 57, 960
BshNI GGYRCC 2 cut(s) 41, 535
BshVI ATCGAT 1 cut(s) 587
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 1 cut(s) 652
BslFI GGGAC 1 cut(s) 751
BslI CCNNNNNNNGG 2 cut(s) 64, 127
BsmFI GGGAC 1 cut(s) 751
BsmI GAATGC 1 cut(s) 237
BsnI GGCC 2 cut(s) 57, 960
Bsp1286I GDGCHC 3 cut(s) 371, 475, 652
Bsp143I GATC 4 cut(s) 142, 559, 803, 1060
BspACI CCGC 2 cut(s) 39, 58
BspANI GGCC 2 cut(s) 57, 960
BspDI ATCGAT 1 cut(s) 587
BspFNI CGCG 3 cut(s) 60, 62, 316
BspLI GGNNCC 4 cut(s) 43, 537, 897, 959
BspMAI CTGCAG 1 cut(s) 1096
BspQI GCTCTTC 1 cut(s) 666
BspT107I GGYRCC 2 cut(s) 41, 535
BsrDI GCAATG 1 cut(s) 58
BsrI ACTGG 5 cut(s) 232, 502, 602, 933, 959
BssMI GATC 4 cut(s) 142, 559, 803, 1060
BssNI GRCGYC 3 cut(s) 21, 42, 78
Bst4CI ACNGT 1 cut(s) 823
Bst6I CTCTTC 2 cut(s) 666, 834
BstACI GRCGYC 3 cut(s) 21, 42, 78
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 1 cut(s) 752
BstF5I GGATG 6 cut(s) 223, 385, 429, 718, 789, 866
BstFNI CGCG 3 cut(s) 60, 62, 316
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 4 cut(s) 44, 62, 259, 316
BstKTI GATC 4 cut(s) 145, 562, 806, 1063
BstMBI GATC 4 cut(s) 142, 559, 803, 1060
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 5 cut(s) 18, 48, 57, 290, 311
BstNSI RCATGY 1 cut(s) 314
BstSFI CTRYAG 2 cut(s) 819, 1092
BstSLI GKGCMC 1 cut(s) 475
BstUI CGCG 3 cut(s) 60, 62, 316
BstV1I GCAGC 1 cut(s) 730
BstV2I GAAGAC 2 cut(s) 169, 600
BstX2I RGATCY 1 cut(s) 1060
BstXI CCANNNNNNTGG 2 cut(s) 200, 301
BstYI RGATCY 1 cut(s) 1060
Bsu15I ATCGAT 1 cut(s) 587
BsuI GTATCC 1 cut(s) 519
BsuRI GGCC 2 cut(s) 57, 960
BsuTUI ATCGAT 1 cut(s) 587
BtrI CACGTC 1 cut(s) 349
BtsCI GGATG 6 cut(s) 223, 385, 429, 718, 789, 866
BtsI GCAGTG 2 cut(s) 924, 1089
BtsIMutI CAGTG 5 cut(s) 790, 924, 940, 952, 1089
Cac8I GCNNGC 1 cut(s) 261
CfoI GCGC 4 cut(s) 44, 62, 259, 316
Cfr13I GGNCC 1 cut(s) 958
ClaI ATCGAT 1 cut(s) 587
CseI GACGC 5 cut(s) 10, 51, 67, 167, 894
CviAII CATG 5 cut(s) 154, 301, 311, 505, 518
DdeI CTNAG 1 cut(s) 752
DinI GGCGCC 1 cut(s) 43
DpnI GATC 4 cut(s) 144, 561, 805, 1062
DpnII GATC 4 cut(s) 142, 559, 803, 1060
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 55
Eam1104I CTCTTC 2 cut(s) 666, 834
EarI CTCTTC 2 cut(s) 666, 834
Eco24I GRGCYC 1 cut(s) 371
Eco57I CTGAAG 2 cut(s) 677, 690
EcoT38I GRGCYC 1 cut(s) 371
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
FaeI CATG 5 cut(s) 157, 304, 314, 508, 521
FalI AAGNNNNNCTT 2 cut(s) 179, 211
FaqI GGGAC 1 cut(s) 751
FatI CATG 5 cut(s) 153, 300, 310, 504, 517
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 4 cut(s) 10, 40, 58, 744
FokI GGATG 6 cut(s) 230, 392, 436, 705, 776, 873
FriOI GRGCYC 1 cut(s) 371
Fsp4HI GCNGC 4 cut(s) 10, 40, 58, 744
FspBI CTAG 2 cut(s) 270, 513
FspI TGCGCA 1 cut(s) 258
GlaI GCGC 4 cut(s) 43, 61, 258, 315
GluI GCNGC 4 cut(s) 10, 40, 58, 744
GsuI CTGGAG 1 cut(s) 519
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 2 cut(s) 57, 960
HgaI GACGC 5 cut(s) 10, 51, 67, 167, 894
HhaI GCGC 4 cut(s) 44, 62, 259, 316
Hin1I GRCGYC 3 cut(s) 21, 42, 78
Hin1II CATG 5 cut(s) 157, 304, 314, 508, 521
Hin6I GCGC 4 cut(s) 42, 60, 257, 314
HinP1I GCGC 4 cut(s) 42, 60, 257, 314
HincII GTYRAC 2 cut(s) 82, 921
HindII GTYRAC 2 cut(s) 82, 921
HinfI GANTC 5 cut(s) 361, 446, 458, 501, 581
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 4 cut(s) 70, 82, 921, 1079
Hpy188I TCNGA 5 cut(s) 31, 142, 418, 696, 834
Hpy188III TCNNGA 3 cut(s) 181, 392, 1012
Hpy8I GTNNAC 4 cut(s) 70, 82, 921, 1079
Hpy99I CGWCG 4 cut(s) 26, 67, 83, 1013
HpyAV CCTTC 4 cut(s) 121, 121, 268, 725
HpyCH4III ACNGT 1 cut(s) 823
HpyCH4IV ACGT 1 cut(s) 348
HpyCH4V TGCA 6 cut(s) 12, 102, 343, 1026, 1046, 1094
HpyF10VI GCNNNNNNNGC 5 cut(s) 18, 48, 57, 290, 311
HpyF3I CTNAG 1 cut(s) 752
HpySE526I ACGT 1 cut(s) 348
Hsp92I GRCGYC 3 cut(s) 21, 42, 78
Hsp92II CATG 5 cut(s) 157, 304, 314, 508, 521
HspAI GCGC 4 cut(s) 42, 60, 257, 314
KasI GGCGCC 1 cut(s) 41
Kzo9I GATC 4 cut(s) 142, 559, 803, 1060
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 2 cut(s) 6, 934
Lsp1109I GCAGC 1 cut(s) 730
LweI GCATC 1 cut(s) 123
MaeI CTAG 2 cut(s) 270, 513
MaeII ACGT 1 cut(s) 348
MaeIII GTNAC 3 cut(s) 476, 785, 823
MalI GATC 4 cut(s) 144, 561, 805, 1062
MboI GATC 4 cut(s) 142, 559, 803, 1060
MboII GAAGA 7 cut(s) 169, 605, 683, 683, 691, 821, 1055
MflI RGATCY 1 cut(s) 1060
MhlI GDGCHC 3 cut(s) 371, 475, 652
MluCI AATT 6 cut(s) 91, 246, 411, 912, 1020, 1049
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 3 cut(s) 452, 455, 510
MmeI TCCRAC 1 cut(s) 195
MnlI CCTC 7 cut(s) 125, 349, 370, 787, 803, 875, 886
MseI TTAA 1 cut(s) 770
MslI CAYNNNNRTG 4 cut(s) 198, 299, 315, 703
MspA1I CMGCKG 1 cut(s) 743
Mva1269I GAATGC 1 cut(s) 237
MvnI CGCG 3 cut(s) 60, 62, 316
MwoI GCNNNNNNNGC 5 cut(s) 18, 48, 57, 290, 311
NarI GGCGCC 1 cut(s) 42
NdeII GATC 4 cut(s) 142, 559, 803, 1060
NlaIII CATG 5 cut(s) 157, 304, 314, 508, 521
NlaIV GGNNCC 4 cut(s) 43, 537, 897, 959
NmuCI GTSAC 2 cut(s) 476, 785
NsbI TGCGCA 1 cut(s) 258
NspI RCATGY 1 cut(s) 314
PciSI GCTCTTC 1 cut(s) 666
PctI GAATGC 1 cut(s) 237
PfeI GAWTC 2 cut(s) 361, 581
PkrI GCNGC 4 cut(s) 11, 41, 59, 745
Ple19I CGATCG 1 cut(s) 145
PleI GAGTC 3 cut(s) 452, 454, 509
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 3 cut(s) 452, 454, 509
PspN4I GGNNCC 4 cut(s) 43, 537, 897, 959
PspPI GGNCC 1 cut(s) 958
PstI CTGCAG 1 cut(s) 1096
PsuI RGATCY 1 cut(s) 1060
PvuI CGATCG 1 cut(s) 145
PvuII CAGCTG 1 cut(s) 743
RseI CAYNNNNRTG 4 cut(s) 198, 299, 315, 703
SalI GTCGAC 1 cut(s) 80
SapI GCTCTTC 1 cut(s) 666
SaqAI TTAA 1 cut(s) 770
SatI GCNGC 4 cut(s) 10, 40, 58, 744
Sau3AI GATC 4 cut(s) 142, 559, 803, 1060
Sau96I GGNCC 1 cut(s) 958
SchI GAGTC 3 cut(s) 452, 455, 510
SduI GDGCHC 3 cut(s) 371, 475, 652
SfaNI GCATC 1 cut(s) 123
SfcI CTRYAG 2 cut(s) 819, 1092
SfoI GGCGCC 1 cut(s) 43
SmiMI CAYNNNNRTG 4 cut(s) 198, 299, 315, 703
Sse9I AATT 6 cut(s) 91, 246, 411, 912, 1020, 1049
SsiI CCGC 2 cut(s) 39, 58
SspDI GGCGCC 1 cut(s) 41
SspI AATATT 2 cut(s) 686, 1038
SspMI CTAG 2 cut(s) 270, 513
TaaI ACNGT 1 cut(s) 823
TaiI ACGT 1 cut(s) 351
TaqI TCGA 6 cut(s) 81, 449, 587, 795, 880, 1011
TaqII GACCGA 1 cut(s) 495
TasI AATT 6 cut(s) 91, 246, 411, 912, 1020, 1049
TauI GCSGC 2 cut(s) 42, 60
TfiI GAWTC 2 cut(s) 361, 581
Tru1I TTAA 1 cut(s) 770
Tru9I TTAA 1 cut(s) 770
TscAI CASTG 5 cut(s) 790, 931, 940, 959, 1096
TseFI GTSAC 2 cut(s) 476, 785
TseI GCWGC 2 cut(s) 9, 743
Tsp45I GTSAC 2 cut(s) 476, 785
TspDTI ATGAA 5 cut(s) 594, 630, 707, 863, 924
TspRI CASTG 5 cut(s) 790, 931, 940, 959, 1096
XapI RAATTY 1 cut(s) 1049
XceI RCATGY 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 2 cut(s) 270, 513
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.