MD15G1188600.v1.1

ADP-ribosylation factor GTPase-activating protein AGD14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006003
Physical Location & Seq
Reverse (-)
14892436 .. 14899111
6676 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1188600.v1.1.491

Sequence Viewer

Length: 2124 bp
ATGGCGAGTCGAAAGGAGGAAGAGAAAAATGAACGAATTATTCGCGGCCTTCTTAAACTTCCGGAGAATCGCAGATGCATCAACTGCAACAGTCTGGGGCCACAGTATGTTTGCACAAATTTCTGGACATTTGTTTGCACGAACTGCAGTGGAATACACCGTGAGTTCACACACCGTGTAAAATCAGTATCGATGGCCAAATTTACTTCACAAGAAGTTAATGCCCTTCAAGAAGGGGGAAATCAGCGTGCAAAGGAAATTTATCATAAAGAATTGGACCTGCAACGTAATTCCTTTCCTGACAGCAGTAATGTTGAGAGACTTCGAGACTTTATTAAGCATGTATACGTGGATAGAAGATATACTGGTGAGAGAAACTTGGACAAGCCTCCAAAGGGGAAAATGGGTGAGAAAGAAGATTCCTATGACAACAGAAGAGTAGATTCATATCAGGGTGGCTCTCGAAGCCCGCCATATGATGATAAATATGACCGTCGTTTCAGTGATAAGTCTAGTCCTGGTGGAAGAAGTTATGATGGGCAAAGAAGTCCGGGATATGATCAAGAAAGTCGGCAGTCTGGTGATTATAGGAGAAGTCCTGGTCGACCAGAAATTGTGAATGATTGGCGTCGAGAAGATAGATTTGGAAATGGGAGTCGAAGTGATGATCGTAGAATATCTGATGGAGATTCTAAAGTGGAAGGAAAATCACCTGAACGACCAAGAGATCTAAATTCATCCAGTCCCCCTATTGTACGGCCTGTTAGAGAGATTTTGGGGGAGAACACAATACCTCTCCGTATAATTGAACCTCCAAAAGCTAACAGTGCTAGAGCTACTGATAGCTTTGTGCATACACAGAGAAATGTGTCGTCCAGCAGCTTGGCCTCCTCTAATGGGAACCCAGTGGAAGTGAAGCCGGAGACTTCTGGAAGTTTAATTGATTTTGATGCTGATATTGAACCTACTCCAGCTGCTGCAGTTGCTCAAGCGCAACAACCTACTGTAGCTCAATCCTTCCCACAGCCAGCAAATTCAACCAATGACAACAATTGGGCCTCTTTTGATGTTGCTCCAGCAGTGACAGTACCTCAAGCTCCTGCGAATGTGAATTCATTAGAATCTCTACTATCACAATTATCAGTTTCAGCACCTGTCCCTAGTTATACTTCTGGGACTGCTGGTAATATTGGTGCTCCCACAGTTGCACCAGTAAGCAGCTTGTCAACATTTCCCCCTGTTGGTGCTCCGGTTCCTGCCCCCGGATCTCAAGCATTCCCAGTAAATGGTGGTAATTTCCAAGTTAAAGATGCTGCTTCTGGCCAATGGCCTACCATGCAGCAACATCAACCTTCATTATACCCTGTTGCTGGTATTCAACCTGCTGCACAACAATACATGCCTTCTGTCGATGGAACTTCGACTATTGCATCACAATCGTCAGCTGGGGATGCAAAATCTACTGGAAGAAGAGAACTTCCTGCGGATATTTTTGCAATGAACTATTCATCCTTTCCTGCACCAGTTCCTGGATGGCAAACTGGTCCTCGTGGTATGGGGTTTCCTATGCCATATAACACTGCAGTGCCCATGCCCAATTATCAGCAGTCTTCAAAGTCAGCAAACCCATTTGATGTAAACCGTGAACCTCAAATACAAGCCTCAGCGTTTCCTTCCTTGCAAGCTCCCCTACCAAATGCCCAGCCTTTATCCCCATCGGGTTTAATGCGCATGTCCAACCTTGGTGCTCCCTCGTCAGCATGGATACCCCTCCAATCATCACCATATTCATCAGCGGTGCCTCCCCAAGCACCACAATATGCATCACAAGTGCCTCTCCAAGCACCACAATATGCATCACAAGTGCCTCCACAGCCACCACAATTTGCATCACAAGTGCCTCCACAGCCACCACAATTTGCATCACAGATGCCACCAAGGGCCTACATGGTGCAACAAGTTCCGAGTAGCATCCCACCTTCCGGGTATCAATGGGCAGGAGGCTTTGGCACCGATGGGGCTTCTTTTGGTTCATTAAATATGGATCAACAGCTGGCAGGTAGATTCTCAGCGGCCGCTACCCCAAATCCTTTCTCTTCAACGGGAGGAAATCCATTTGGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArfGap

Protein Analysis

708

Amino Acids

76.47

Weight (kDa)

8.4

Isoelectric Point (pI)

73.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 12 - 122 1.4e-29 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1730
Acc36I ACCTGC 3 cut(s) 288, 1390, 2048
AccB1I GGYRCC 2 cut(s) 1798, 2009
AccB7I CCANNNNNTGG 2 cut(s) 1286, 1529
AccI GTMKAC 2 cut(s) 345, 604
AccII CGCG 1 cut(s) 45
AccIII TCCGGA 1 cut(s) 61
AciI CCGC 6 cut(s) 45, 470, 1484, 1796, 2072, 2076
AclWI GGATC 2 cut(s) 1273, 2052
AcoI YGGCCR 3 cut(s) 195, 1321, 2073
AcsI RAATTY 6 cut(s) 118, 200, 258, 733, 1033, 1111
AcyI GRCGYC 1 cut(s) 628
AdeI CACNNNGTG 1 cut(s) 176
AfaI GTAC 2 cut(s) 756, 1089
AfiI CCNNNNNNNGG 8 cut(s) 395, 897, 1241, 1262, 1286, 1370, 1529, 1982
AgsI TTSAA 7 cut(s) 230, 809, 962, 1038, 1379, 1614, 2100
AjnI CCWGG 3 cut(s) 517, 598, 1528
AjuI GAANNNNNNNTTGG 2 cut(s) 627, 659
AleI CACNNNNGTG 1 cut(s) 1583
AloI GAACNNNNNNTCC 2 cut(s) 1493, 1525
Alw21I GWGCWC 3 cut(s) 1198, 1249, 1750
Alw26I GTCTC 3 cut(s) 313, 321, 917
AlwI GGATC 2 cut(s) 1273, 2052
AlwNI CAGNNNCTG 3 cut(s) 977, 1154, 1529
Aor13HI TCCGGA 1 cut(s) 61
ApeKI GCWGC 7 cut(s) 879, 974, 977, 1218, 1313, 1339, 1385
ApoI RAATTY 6 cut(s) 118, 200, 258, 733, 1033, 1111
AspLEI GCGC 2 cut(s) 994, 1731
AspS9I GGNCC 5 cut(s) 98, 277, 1056, 1544, 1941
AsuC2I CCSGG 3 cut(s) 552, 1263, 1984
AsuHPI GGTGA 5 cut(s) 380, 419, 593, 702, 1773
AvaII GGWCC 2 cut(s) 277, 1544
BaeGI GKGCMC 1 cut(s) 1590
BalI TGGCCA 2 cut(s) 197, 1323
BanI GGYRCC 2 cut(s) 1798, 2009
BauI CACGAG 1 cut(s) 1548
BbsI GAAGAC 1 cut(s) 1602
Bbv12I GWGCWC 3 cut(s) 1198, 1249, 1750
BbvCI CCTCAGC 1 cut(s) 1663
BbvI GCAGC 7 cut(s) 891, 961, 964, 1230, 1300, 1351, 1372
BccI CCATC 7 cut(s) 187, 530, 677, 1406, 1527, 1723, 2009
BceAI ACGGC 1 cut(s) 773
BcgI CGANNNNNNTGC 2 cut(s) 1419, 1453
BciT130I CCWGG 3 cut(s) 519, 600, 1530
BciVI GTATCC 1 cut(s) 1758
BclI TGATCA 1 cut(s) 559
BcnI CCSGG 3 cut(s) 552, 1263, 1984
BcoDI GTCTC 3 cut(s) 313, 321, 917
BfaI CTAG 3 cut(s) 513, 831, 1161
BfmI CTRYAG 4 cut(s) 145, 978, 1005, 1581
BfuAI ACCTGC 3 cut(s) 288, 1390, 2048
BfuI GTATCC 1 cut(s) 1758
BglII AGATCT 1 cut(s) 727
Bme1390I CCNGG 6 cut(s) 519, 552, 600, 1263, 1530, 1984
Bme18I GGWCC 2 cut(s) 277, 1544
BmgT120I GGNCC 5 cut(s) 98, 277, 1056, 1544, 1941
BmiI GGNNCC 5 cut(s) 99, 902, 1254, 1800, 2011
BmrFI CCNGG 6 cut(s) 519, 552, 600, 1263, 1530, 1984
BmrI ACTGGG 2 cut(s) 899, 1274
BmuI ACTGGG 2 cut(s) 899, 1274
BpiI GAAGAC 1 cut(s) 1602
BpmI CTGGAG 2 cut(s) 954, 1059
Bpu10I CCTNAGC 1 cut(s) 1663
BpuEI CTTGAG 3 cut(s) 972, 1077, 1254
BpuMI CCSGG 3 cut(s) 552, 1263, 1984
Bsa29I ATCGAT 1 cut(s) 191
BsaAI YACGTR 1 cut(s) 349
BsaBI GATNNNNATC 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 628
BsaJI CCNNGG 3 cut(s) 1261, 1741, 1937
BsaWI WCCGGW 2 cut(s) 61, 1249
BsaXI ACNNNNNCTCC 2 cut(s) 646, 676
Bsc4I CCNNNNNNNGG 8 cut(s) 395, 897, 1241, 1262, 1286, 1370, 1529, 1982
Bse1I ACTGG 8 cut(s) 370, 741, 905, 1211, 1280, 1468, 1523, 1546
Bse3DI GCAATG 1 cut(s) 1503
Bse8I GATNNNNATC 1 cut(s) 447
BseAI TCCGGA 1 cut(s) 61
BseBI CCWGG 3 cut(s) 519, 600, 1530
BseCI ATCGAT 1 cut(s) 191
BseDI CCNNGG 3 cut(s) 1261, 1741, 1937
BseGI GGATG 5 cut(s) 737, 1456, 1508, 1538, 1971
BseJI GATNNNNATC 1 cut(s) 447
BseLI CCNNNNNNNGG 8 cut(s) 395, 897, 1241, 1262, 1286, 1370, 1529, 1982
BseMI GCAATG 1 cut(s) 1503
BseMII CTCAG 2 cut(s) 1677, 2082
BseNI ACTGG 8 cut(s) 370, 741, 905, 1211, 1280, 1468, 1523, 1546
BseRI GAGGAG 1 cut(s) 880
BseSI GKGCMC 1 cut(s) 1590
BseX3I CGGCCG 1 cut(s) 2073
BseXI GCAGC 7 cut(s) 891, 961, 964, 1230, 1300, 1351, 1372
BseYI CCCAGC 2 cut(s) 1445, 1701
BsgI GTGCAG 2 cut(s) 1371, 1503
Bsh1236I CGCG 1 cut(s) 45
Bsh1285I CGRYCG 1 cut(s) 2076
BshNI GGYRCC 2 cut(s) 1798, 2009
BshVI ATCGAT 1 cut(s) 191
BsiEI CGRYCG 1 cut(s) 2076
BsiHKAI GWGCWC 3 cut(s) 1198, 1249, 1750
BsiSI CCGG 6 cut(s) 62, 551, 920, 1250, 1263, 1983
BslFI GGGAC 3 cut(s) 729, 1142, 1189
BslI CCNNNNNNNGG 8 cut(s) 395, 897, 1241, 1262, 1286, 1370, 1529, 1982
BsmAI GTCTC 3 cut(s) 313, 321, 917
BsmFI GGGAC 3 cut(s) 729, 1142, 1189
BsmI GAATGC 1 cut(s) 1274
Bsp1286I GDGCHC 4 cut(s) 1198, 1249, 1590, 1750
Bsp13I TCCGGA 1 cut(s) 61
Bsp143I GATC 5 cut(s) 559, 667, 727, 1265, 2044
BspACI CCGC 6 cut(s) 45, 470, 1484, 1796, 2072, 2076
BspCNI CTCAG 2 cut(s) 1676, 2081
BspDI ATCGAT 1 cut(s) 191
BspEI TCCGGA 1 cut(s) 61
BspFNI CGCG 1 cut(s) 45
BspLI GGNNCC 5 cut(s) 99, 902, 1254, 1800, 2011
BspMAI CTGCAG 3 cut(s) 149, 982, 1585
BspMI ACCTGC 3 cut(s) 288, 1390, 2048
BspPI GGATC 2 cut(s) 1273, 2052
BspT107I GGYRCC 2 cut(s) 1798, 2009
BsrDI GCAATG 1 cut(s) 1503
BsrI ACTGG 8 cut(s) 370, 741, 905, 1211, 1280, 1468, 1523, 1546
BssECI CCNNGG 3 cut(s) 1261, 1741, 1937
BssMI GATC 5 cut(s) 559, 667, 727, 1265, 2044
BssNAI GTATAC 1 cut(s) 346
BssNI GRCGYC 1 cut(s) 628
BssSI CACGAG 1 cut(s) 1548
BssT1I CCWWGG 2 cut(s) 1741, 1937
Bst1107I GTATAC 1 cut(s) 346
Bst2BI CACGAG 1 cut(s) 1548
Bst2UI CCWGG 3 cut(s) 519, 600, 1530
Bst6I CTCTTC 4 cut(s) 15, 430, 1465, 2101
BstACI GRCGYC 1 cut(s) 628
BstAPI GCANNNNNTGC 2 cut(s) 84, 144
BstBAI YACGTR 1 cut(s) 349
BstC8I GCNNGC 5 cut(s) 249, 470, 1029, 1683, 2055
BstDEI CTNAG 2 cut(s) 1663, 2068
BstF5I GGATG 5 cut(s) 737, 1456, 1508, 1538, 1971
BstFNI CGCG 1 cut(s) 45
BstHHI GCGC 2 cut(s) 994, 1731
BstKTI GATC 5 cut(s) 562, 670, 730, 1268, 2047
BstMAI GTCTC 3 cut(s) 313, 321, 917
BstMBI GATC 5 cut(s) 559, 667, 727, 1265, 2044
BstMCI CGRYCG 1 cut(s) 2076
BstMWI GCNNNNNNNGC 9 cut(s) 84, 144, 465, 827, 983, 1336, 1451, 1873, 1906
BstNI CCWGG 3 cut(s) 519, 600, 1530
BstNSI RCATGY 3 cut(s) 344, 1402, 1735
BstSCI CCNGG 6 cut(s) 517, 550, 598, 1261, 1528, 1982
BstSFI CTRYAG 4 cut(s) 145, 978, 1005, 1581
BstSLI GKGCMC 1 cut(s) 1590
BstUI CGCG 1 cut(s) 45
BstV1I GCAGC 7 cut(s) 891, 961, 964, 1230, 1300, 1351, 1372
BstV2I GAAGAC 1 cut(s) 1602
BstX2I RGATCY 2 cut(s) 727, 1265
BstXI CCANNNNNNTGG 1 cut(s) 883
BstYI RGATCY 2 cut(s) 727, 1265
BstZ17I GTATAC 1 cut(s) 346
BstZI CGGCCG 1 cut(s) 2073
Bsu15I ATCGAT 1 cut(s) 191
BsuI GTATCC 1 cut(s) 1758
BsuTUI ATCGAT 1 cut(s) 191
BtsCI GGATG 5 cut(s) 737, 1456, 1508, 1538, 1971
BtsI GCAGTG 4 cut(s) 154, 1086, 1578, 1590
BtsIMutI CAGTG 7 cut(s) 154, 508, 832, 912, 1086, 1578, 1590
BveI ACCTGC 3 cut(s) 288, 1390, 2048
Cac8I GCNNGC 5 cut(s) 249, 470, 1029, 1683, 2055
CaiI CAGNNNCTG 3 cut(s) 977, 1154, 1529
CciNI GCGGCCGC 1 cut(s) 2073
CfoI GCGC 2 cut(s) 994, 1731
Cfr13I GGNCC 5 cut(s) 98, 277, 1056, 1544, 1941
ClaI ATCGAT 1 cut(s) 191
CseI GACGC 1 cut(s) 617
Csp6I GTAC 2 cut(s) 755, 1088
CviAII CATG 7 cut(s) 341, 1336, 1399, 1591, 1732, 1761, 1948
CviQI GTAC 2 cut(s) 755, 1088
DdeI CTNAG 2 cut(s) 1663, 2068
DpnI GATC 5 cut(s) 561, 669, 729, 1267, 2046
DpnII GATC 5 cut(s) 559, 667, 727, 1265, 2044
DraIII CACNNNGTG 1 cut(s) 176
EaeI YGGCCR 3 cut(s) 195, 1321, 2073
EagI CGGCCG 1 cut(s) 2073
Eam1104I CTCTTC 4 cut(s) 15, 430, 1465, 2101
EarI CTCTTC 4 cut(s) 15, 430, 1465, 2101
EclXI CGGCCG 1 cut(s) 2073
Eco130I CCWWGG 2 cut(s) 1741, 1937
Eco47I GGWCC 2 cut(s) 277, 1544
Eco52I CGGCCG 1 cut(s) 2073
EcoO109I RGGNCCY 1 cut(s) 1941
EcoRI GAATTC 1 cut(s) 1111
EcoRII CCWGG 3 cut(s) 517, 598, 1528
EcoT14I CCWWGG 2 cut(s) 1741, 1937
EcoT22I ATGCAT 3 cut(s) 80, 1825, 1858
ErhI CCWWGG 2 cut(s) 1741, 1937
FaeI CATG 7 cut(s) 344, 1339, 1402, 1594, 1735, 1764, 1951
FaqI GGGAC 3 cut(s) 729, 1142, 1189
FatI CATG 7 cut(s) 340, 1335, 1398, 1590, 1731, 1760, 1947
FauI CCCGC 1 cut(s) 477
FauNDI CATATG 1 cut(s) 475
FbaI TGATCA 1 cut(s) 559
FblI GTMKAC 2 cut(s) 345, 604
FokI GGATG 5 cut(s) 724, 1463, 1495, 1545, 1958
FspAI RTGCGCAY 1 cut(s) 1730
FspBI CTAG 3 cut(s) 513, 831, 1161
FspI TGCGCA 1 cut(s) 1730
GlaI GCGC 2 cut(s) 993, 1730
GsaI CCCAGC 2 cut(s) 1449, 1705
GsuI CTGGAG 2 cut(s) 954, 1059
HapII CCGG 6 cut(s) 62, 551, 920, 1250, 1263, 1983
HgaI GACGC 1 cut(s) 617
HhaI GCGC 2 cut(s) 994, 1731
Hin1I GRCGYC 1 cut(s) 628
Hin1II CATG 7 cut(s) 344, 1339, 1402, 1594, 1735, 1764, 1951
Hin6I GCGC 2 cut(s) 992, 1729
HinP1I GCGC 2 cut(s) 992, 1729
HincII GTYRAC 2 cut(s) 605, 1227
HindII GTYRAC 2 cut(s) 605, 1227
HinfI GANTC 8 cut(s) 7, 67, 419, 443, 655, 689, 1121, 2064
HpaII CCGG 6 cut(s) 62, 551, 920, 1250, 1263, 1983
HphI GGTGA 5 cut(s) 380, 419, 593, 702, 1773
Hpy166II GTNNAC 6 cut(s) 168, 346, 605, 1227, 1639, 1646
Hpy188I TCNGA 2 cut(s) 682, 1965
Hpy188III TCNNGA 9 cut(s) 62, 124, 230, 299, 326, 462, 563, 632, 930
Hpy8I GTNNAC 6 cut(s) 168, 346, 605, 1227, 1639, 1646
Hpy99I CGWCG 2 cut(s) 498, 633
HpyAV CCTTC 9 cut(s) 59, 227, 236, 695, 1027, 1362, 1413, 1683, 1989
HpyCH4IV ACGT 2 cut(s) 286, 348
HpyF10VI GCNNNNNNNGC 9 cut(s) 84, 144, 465, 827, 983, 1336, 1451, 1873, 1906
HpyF3I CTNAG 2 cut(s) 1663, 2068
HpySE526I ACGT 2 cut(s) 286, 348
Hsp92I GRCGYC 1 cut(s) 628
Hsp92II CATG 7 cut(s) 344, 1339, 1402, 1594, 1735, 1764, 1951
HspAI GCGC 2 cut(s) 992, 1729
Kpn2I TCCGGA 1 cut(s) 61
Ksp22I TGATCA 1 cut(s) 559
Kzo9I GATC 5 cut(s) 559, 667, 727, 1265, 2044
LmnI GCTCC 6 cut(s) 1078, 1102, 1201, 1252, 1690, 1753
Lsp1109I GCAGC 7 cut(s) 891, 961, 964, 1230, 1300, 1351, 1372
MaeI CTAG 3 cut(s) 513, 831, 1161
MaeII ACGT 2 cut(s) 286, 348
MaeIII GTNAC 1 cut(s) 1081
MalI GATC 5 cut(s) 561, 669, 729, 1267, 2046
MboI GATC 5 cut(s) 559, 667, 727, 1265, 2044
MfeI CAATTG 1 cut(s) 1051
MflI RGATCY 2 cut(s) 727, 1265
MhlI GDGCHC 4 cut(s) 1198, 1249, 1590, 1750
MlsI TGGCCA 2 cut(s) 197, 1323
MluNI TGGCCA 2 cut(s) 197, 1323
MlyI GAGTC 2 cut(s) 16, 664
MmeI TCCRAC 1 cut(s) 1761
Mox20I TGGCCA 2 cut(s) 197, 1323
Mph1103I ATGCAT 3 cut(s) 80, 1825, 1858
MroI TCCGGA 1 cut(s) 61
MscI TGGCCA 2 cut(s) 197, 1323
MseI TTAA 7 cut(s) 54, 219, 336, 938, 1305, 1724, 2036
MslI CAYNNNNRTG 1 cut(s) 1583
Msp20I TGGCCA 2 cut(s) 197, 1323
MspA1I CMGCKG 5 cut(s) 974, 1445, 1796, 2053, 2072
MspI CCGG 6 cut(s) 62, 551, 920, 1250, 1263, 1983
MspR9I CCNGG 6 cut(s) 519, 552, 600, 1263, 1530, 1984
MunI CAATTG 1 cut(s) 1051
Mva1269I GAATGC 1 cut(s) 1274
MvaI CCWGG 3 cut(s) 519, 600, 1530
MvnI CGCG 1 cut(s) 45
MwoI GCNNNNNNNGC 9 cut(s) 84, 144, 465, 827, 983, 1336, 1451, 1873, 1906
NciI CCSGG 3 cut(s) 552, 1263, 1984
NdeI CATATG 1 cut(s) 475
NdeII GATC 5 cut(s) 559, 667, 727, 1265, 2044
NlaIII CATG 7 cut(s) 344, 1339, 1402, 1594, 1735, 1764, 1951
NlaIV GGNNCC 5 cut(s) 99, 902, 1254, 1800, 2011
NmuCI GTSAC 1 cut(s) 1081
NotI GCGGCCGC 1 cut(s) 2073
NsbI TGCGCA 1 cut(s) 1730
NsiI ATGCAT 3 cut(s) 80, 1825, 1858
NspI RCATGY 3 cut(s) 344, 1402, 1735
OliI CACNNNNGTG 1 cut(s) 1583
PctI GAATGC 1 cut(s) 1274
PfeI GAWTC 6 cut(s) 67, 419, 443, 689, 1121, 2064
PflMI CCANNNNNTGG 2 cut(s) 1286, 1529
PfoI TCCNGGA 2 cut(s) 550, 1528
PleI GAGTC 2 cut(s) 15, 663
PpsI GAGTC 2 cut(s) 15, 663
Ppu21I YACGTR 1 cut(s) 349
Psp6I CCWGG 3 cut(s) 517, 598, 1528
PspFI CCCAGC 2 cut(s) 1445, 1701
PspGI CCWGG 3 cut(s) 517, 598, 1528
PspN4I GGNNCC 5 cut(s) 99, 902, 1254, 1800, 2011
PspPI GGNCC 5 cut(s) 98, 277, 1056, 1544, 1941
PsrI GAACNNNNNNTAC 2 cut(s) 1638, 1670
PstI CTGCAG 3 cut(s) 149, 982, 1585
PstNI CAGNNNCTG 3 cut(s) 977, 1154, 1529
PsuI RGATCY 2 cut(s) 727, 1265
PvuII CAGCTG 3 cut(s) 974, 1445, 2053
RsaI GTAC 2 cut(s) 756, 1089
RsaNI GTAC 2 cut(s) 755, 1088
RseI CAYNNNNRTG 1 cut(s) 1583
SalI GTCGAC 1 cut(s) 603
SaqAI TTAA 7 cut(s) 54, 219, 336, 938, 1305, 1724, 2036
Sau3AI GATC 5 cut(s) 559, 667, 727, 1265, 2044
Sau96I GGNCC 5 cut(s) 98, 277, 1056, 1544, 1941
SchI GAGTC 2 cut(s) 16, 664
ScrFI CCNGG 6 cut(s) 519, 552, 600, 1263, 1530, 1984
SduI GDGCHC 4 cut(s) 1198, 1249, 1590, 1750
SfcI CTRYAG 4 cut(s) 145, 978, 1005, 1581
SinI GGWCC 2 cut(s) 277, 1544
SmiMI CAYNNNNRTG 1 cut(s) 1583
SmlI CTYRAG 3 cut(s) 987, 1092, 1269
SmoI CTYRAG 3 cut(s) 987, 1092, 1269
SsiI CCGC 6 cut(s) 45, 470, 1484, 1796, 2072, 2076
SspI AATATT 1 cut(s) 1189
SspMI CTAG 3 cut(s) 513, 831, 1161
StyD4I CCNGG 6 cut(s) 517, 550, 598, 1261, 1528, 1982
StyI CCWWGG 2 cut(s) 1741, 1937
TaiI ACGT 2 cut(s) 289, 351
TaqI TCGA 9 cut(s) 10, 191, 325, 463, 604, 631, 658, 1410, 1421
TauI GCSGC 3 cut(s) 48, 2075, 2078
TfiI GAWTC 6 cut(s) 67, 419, 443, 689, 1121, 2064
Tru1I TTAA 7 cut(s) 54, 219, 336, 938, 1305, 1724, 2036
Tru9I TTAA 7 cut(s) 54, 219, 336, 938, 1305, 1724, 2036
TscAI CASTG 7 cut(s) 154, 508, 832, 912, 1086, 1585, 1590
TseFI GTSAC 1 cut(s) 1081
TseI GCWGC 7 cut(s) 879, 974, 977, 1218, 1313, 1339, 1385
Tsp45I GTSAC 1 cut(s) 1081
TspDTI ATGAA 9 cut(s) 45, 435, 726, 1104, 1344, 1497, 1514, 1779, 2022
TspGWI ACGGA 1 cut(s) 788
TspRI CASTG 7 cut(s) 154, 508, 832, 912, 1086, 1585, 1590
Van91I CCANNNNNTGG 2 cut(s) 1286, 1529
VpaK11BI GGWCC 2 cut(s) 277, 1544
XapI RAATTY 6 cut(s) 118, 200, 258, 733, 1033, 1111
XceI RCATGY 3 cut(s) 344, 1402, 1735
XcmI CCANNNNNNNNNTGG 1 cut(s) 1530
XmiI GTMKAC 2 cut(s) 345, 604
XspI CTAG 3 cut(s) 513, 831, 1161
Zsp2I ATGCAT 3 cut(s) 80, 1825, 1858
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.