RLG00000016162

ADP-ribosylation factor GTPase-activating protein AGD14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006003
Physical Location & Seq
Reverse (-)
4376173 .. 4383490
7318 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016162

Sequence Viewer

Length: 2316 bp
ATGGTTGTAGAAGTTCAAAAGGTCAATGTAATTCAAACGAAAGGAAAAGTCCATAGATCTCCGCAACGACGTCGTTTGCTAGAACCGCACCGGAGCTTCTTCGAGGAAGTCAAAATGGCGAGCCGAGTGAAGGAGGACGAGAAAAATGAACGGATTATTCGAGGCCTTCTTAAACTTCCTGAGAATCGCAGATGCATTAACTGCAATAGTCTGGGACCACAGTATGTTTGCACGAATTTCTGGACATTTGTTTGCACCAACTGCAGTGGAATACATCGGGAGTTCACACACCGAGTAAAATCAGTGTCAATGGCCAAGTTTACATCGCAAGAAGTTAAATCCCTTCAAGAAGGGGGAAACCAGCGTGCAAAGGAACTTTATCAAAAAGAATTAGATCCACAACGTAATTCCTTCCCTGATAGCAGTAATGTTGAGAGACTTCGAGACTTTATTAAGCATGTGTACGTGGATAGAAGATATACTGGTGAAAGAAACTTGGACAAGCCTCCGAGGGTGAAAATGGGTGACAAGGAGGACTCTTATGAAAATAGAAGGCTAGATTCATATCAGGGTTCTCGAAGCCCACCATATGATGATGAACGTCGTTATAGTGGAAGGTCCAGTCCCGGCGGTAGAAGTTATGAGGAGCAAAGAAGTCCCGGATATGATCTAGAAAGTCGACAGTATGGTGATTCTAAGAGAAGTCCTTCTCGCCCTGAAATTGTCAATGATTGGCGTCGAGAAGATAGATTTGGAAATGGTAGGAGATATGATGACCGTAGAGTATCCGATGGAGATTCTAAACTGGACGCAAAATCTCCTGAACGACCAAGAGATATAGATTTGTCCAGTCCCCCTATGGTACGACCTGTTAGAGATATTTTGGGGGATAAGACAATACCTCTCCGTATTATTGAACCTCCTAAAGCCAACAATGTTAGGCCTACTGAAGGTTCTGTGCTTACACAGAGAACTGCTTCTTCCAGTAGCTTGGGTTCCACCAATGGGAACCCAGTTGAAGTGAAAGTGGAGACTTCTGGAAGTTTAATTGACTTTGATGCCGATATTGAACCTGCTCCAACTCCAGCAGTTTCTCAAGCACAACAAACTTCTGTAACTCAATCATATTCACAGCCAGAAAATTCACACAGTGACAACAACTGGGCCTCCTTTGATGTTGCTCCACAAGTGAAAGTATCTCAAGCTCCTGCAAATGCAAATTCTTTGGAATCTCTGCTATCAGAATTGTCTGTTTCAGCACCTGTCCCTAGTTATTCTGGAACCGCAGATAATACCGGGGCTTTTACAGCTACAGGTAACATGACTGCACCATTCAGTGGTAATTCAGTCATCACTCCTGTAGGACACACAATGTTGCCCGCTACTTCTCGTACAGTTGCACCGACTACGAGCTTGTCAACATTCCCCCCTGTTGGTGCTCCAGTTGCAACCCCTGGATTGGCTCAAACATTCCCTGCTAATGCTAGTAATTTCCCTGCTACCGGTTCCGGGCAATGGCCTAGTATGCAGCATCAGCAGCCTTCTTTATTCCCTGCTAATGGTATTCAGTCTGCCTCTCAACAGTACATACCATCAGTTGGTGGACCTTCAAGCAATCAACCATGGAATTTAGCACATGCCCCAAATGCACAAGGTCAACCTAGCTATCCAGCTGCACAAGCACCACAAGATGTTTCAAGATCTACCCACAATGTTAGTACTGTTGCATCACAAAATTCGGCTGGGGATGTAAAATCAATTGGAAGAAGTGCACTTCCTGAGGATCTTTTTGCTATGAACTATTCATCCTTCCCTGCACCAGTTCCTGGATGGCAAACTGGTCCTCCTCAGGGTATGGGGTTTGCTATGCCATACAACACTGCAATGCCCATGCCCAGTTTTCAGCAGTTGAAGTCAGCAAACCCGTTTGATGTCAATAGTGGACCTCCTCCAGTTCAAGCCTCAGCATTTCCTTCCATGGCATCCTCACAAGTACCGCTACCAAATATGCAGCCTCCATCTGGCTTATTGCGTACAGCTAGCCTTGGGACACCATCATCAGCTTGGGGGCCCTCATATTCATCTGGATTGCCTCTTCAAGTACCACCATATGCATCACAAATGCCTCCAAGTGCCTATGGGGGGCAACAAATTCCGAGTAGCTTGCCACCTTCAGGGTATCAAGGAGCAGGGGGCTTTGGTGCTGAGGGAGCTGCTTTTGGTTCATTAAATATGGATCAACAGCCAGTTGGTAGATTCTCAGCACCTGCTACCCCAAATCCTTTCCCATCTGCAGGAGGAAACCCATTTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArfGap

Protein Analysis

772

Amino Acids

83.36

Weight (kDa)

8.83

Isoelectric Point (pI)

66.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArfGap PF01412 51 - 161 4.9e-30 Putative GTPase activating protein for Arf
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2275
AatII GACGTC 1 cut(s) 73
Acc36I ACCTGC 2 cut(s) 1081, 2275
AccB7I CCANNNNNTGG 3 cut(s) 1337, 1598, 1826
AccI GTMKAC 1 cut(s) 679
AciI CCGC 6 cut(s) 62, 86, 630, 1284, 1380, 1997
AclWI GGATC 3 cut(s) 389, 1791, 2244
AcoI YGGCCR 1 cut(s) 312
AcsI RAATTY 6 cut(s) 235, 1141, 1219, 1627, 1735, 2151
AcuI CTGAAG 2 cut(s) 969, 2157
AcyI GRCGYC 2 cut(s) 70, 736
AdeI CACNNNGTG 1 cut(s) 1151
AfaI GTAC 8 cut(s) 464, 864, 1393, 1586, 1720, 1995, 2035, 2103
AgeI ACCGGT 1 cut(s) 1502
AjnI CCWGG 2 cut(s) 1453, 1825
AjuI GAANNNNNNNTTGG 2 cut(s) 735, 767
AloI GAACNNNNNNTCC 2 cut(s) 1790, 1822
Alw21I GWGCWC 2 cut(s) 1441, 1774
Alw26I GTCTC 3 cut(s) 430, 438, 1025
Alw44I GTGCAC 1 cut(s) 1770
AlwI GGATC 3 cut(s) 389, 1791, 2244
AlwNI CAGNNNCTG 3 cut(s) 1262, 1826, 2267
AoxI GGCC 6 cut(s) 163, 312, 941, 1164, 1517, 2069
ApaI GGGCCC 1 cut(s) 2073
ApaLI GTGCAC 1 cut(s) 1770
ApeKI GCWGC 5 cut(s) 1528, 1537, 1673, 2011, 2213
ApoI RAATTY 6 cut(s) 235, 1141, 1219, 1627, 1735, 2151
AsiGI ACCGGT 1 cut(s) 1502
Asp700I GAANNNNTTC 2 cut(s) 706, 976
AspS9I GGNCC 8 cut(s) 215, 618, 1164, 1604, 1841, 1943, 2069, 2070
AsuC2I CCSGG 4 cut(s) 627, 660, 1297, 1510
AsuHPI GGTGA 4 cut(s) 497, 526, 536, 701
AsuNHI GCTAGC 1 cut(s) 2039
AvaII GGWCC 5 cut(s) 215, 618, 1604, 1841, 1943
AxyI CCTNAGG 2 cut(s) 1779, 1848
BaeGI GKGCMC 2 cut(s) 1774, 2073
BalI TGGCCA 1 cut(s) 314
BanII GRGCYC 1 cut(s) 2073
Bbv12I GWGCWC 2 cut(s) 1441, 1774
BbvCI CCTCAGC 2 cut(s) 1963, 2205
BbvI GCAGC 5 cut(s) 1540, 1549, 1660, 2023, 2200
BccI CCATC 6 cut(s) 785, 1600, 1824, 2026, 2062, 2296
BcgI CGANNNNNNTGC 4 cut(s) 53, 87, 2146, 2180
BciT130I CCWGG 2 cut(s) 1455, 1827
BciVI GTATCC 1 cut(s) 796
BcnI CCSGG 4 cut(s) 627, 660, 1297, 1510
BcoDI GTCTC 3 cut(s) 430, 438, 1025
BfaI CTAG 8 cut(s) 80, 557, 671, 1269, 1485, 1521, 1662, 2040
BfmI CTRYAG 4 cut(s) 262, 1311, 1359, 2292
BfuAI ACCTGC 2 cut(s) 1081, 2275
BfuI GTATCC 1 cut(s) 796
BglII AGATCT 2 cut(s) 56, 1700
BisI GCNGC 5 cut(s) 1529, 1538, 1674, 2012, 2214
BlsI GCNGC 5 cut(s) 1530, 1539, 1675, 2013, 2215
BmcAI AGTACT 1 cut(s) 1720
Bme1390I CCNGG 6 cut(s) 627, 660, 1297, 1455, 1510, 1827
Bme18I GGWCC 5 cut(s) 215, 618, 1604, 1841, 1943
BmgT120I GGNCC 8 cut(s) 215, 618, 1164, 1604, 1841, 1943, 2069, 2070
BmiI GGNNCC 7 cut(s) 216, 997, 1010, 1282, 1507, 2070, 2071
BmrFI CCNGG 6 cut(s) 627, 660, 1297, 1455, 1510, 1827
BmrI ACTGGG 3 cut(s) 1007, 1171, 1890
BmsI GCATC 6 cut(s) 182, 1048, 1540, 1736, 1991, 2123
BmtI GCTAGC 1 cut(s) 2043
BmuI ACTGGG 3 cut(s) 1007, 1171, 1890
BpmI CTGGAG 3 cut(s) 1068, 1425, 1935
Bpu10I CCTNAGC 2 cut(s) 1963, 2205
BpuEI CTTGAG 2 cut(s) 1080, 1185
BpuMI CCSGG 4 cut(s) 627, 660, 1297, 1510
BsaAI YACGTR 1 cut(s) 466
BsaBI GATNNNNATC 1 cut(s) 564
BsaHI GRCGYC 2 cut(s) 70, 736
BsaJI CCNNGG 6 cut(s) 509, 1296, 1453, 1622, 1977, 2044
BsaWI WCCGGW 2 cut(s) 90, 1502
BsaXI ACNNNNNCTCC 4 cut(s) 1151, 1181, 1828, 1858
Bse118I RCCGGY 1 cut(s) 1502
Bse21I CCTNAGG 2 cut(s) 1779, 1848
Bse3DI GCAATG 2 cut(s) 1520, 1890
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 2 cut(s) 1455, 1827
BseDI CCNNGG 6 cut(s) 509, 1296, 1453, 1622, 1977, 2044
BseGI GGATG 4 cut(s) 1753, 1805, 1835, 1982
BseJI GATNNNNATC 1 cut(s) 564
BseMI GCAATG 2 cut(s) 1520, 1890
BseMII CTCAG 6 cut(s) 171, 1770, 1862, 1977, 2196, 2274
BseRI GAGGAG 3 cut(s) 659, 1836, 1938
BseSI GKGCMC 2 cut(s) 1774, 2073
BseXI GCAGC 5 cut(s) 1540, 1549, 1660, 2023, 2200
BseYI CCCAGC 1 cut(s) 1742
BsgI GTGCAG 3 cut(s) 1311, 1659, 1800
BshFI GGCC 6 cut(s) 165, 314, 943, 1166, 1519, 2071
BshTI ACCGGT 1 cut(s) 1502
BsiHKAI GWGCWC 2 cut(s) 1441, 1774
BsiSI CCGG 6 cut(s) 91, 627, 660, 1296, 1503, 1509
BslFI GGGAC 6 cut(s) 228, 609, 642, 837, 1250, 2062
BsmAI GTCTC 3 cut(s) 430, 438, 1025
BsmFI GGGAC 6 cut(s) 228, 609, 642, 837, 1250, 2062
BsnI GGCC 6 cut(s) 165, 314, 943, 1166, 1519, 2071
Bsp120I GGGCCC 1 cut(s) 2069
Bsp1286I GDGCHC 3 cut(s) 1441, 1774, 2073
Bsp143I GATC 6 cut(s) 56, 394, 667, 1700, 1783, 2236
Bsp19I CCATGG 2 cut(s) 1622, 1977
BspACI CCGC 6 cut(s) 62, 86, 630, 1284, 1380, 1997
BspANI GGCC 6 cut(s) 165, 314, 943, 1166, 1519, 2071
BspCNI CTCAG 6 cut(s) 172, 1771, 1861, 1976, 2197, 2273
BspLI GGNNCC 7 cut(s) 216, 997, 1010, 1282, 1507, 2070, 2071
BspMAI CTGCAG 2 cut(s) 266, 2296
BspMI ACCTGC 2 cut(s) 1081, 2275
BspOI GCTAGC 1 cut(s) 2043
BspPI GGATC 3 cut(s) 389, 1791, 2244
BsrDI GCAATG 2 cut(s) 1520, 1890
BsrFI RCCGGY 1 cut(s) 1502
BssAI RCCGGY 1 cut(s) 1502
BssECI CCNNGG 6 cut(s) 509, 1296, 1453, 1622, 1977, 2044
BssMI GATC 6 cut(s) 56, 394, 667, 1700, 1783, 2236
BssNI GRCGYC 2 cut(s) 70, 736
BssT1I CCWWGG 3 cut(s) 1622, 1977, 2044
Bst2UI CCWGG 2 cut(s) 1455, 1827
Bst4CI ACNGT 7 cut(s) 222, 684, 779, 1151, 1396, 1584, 1723
Bst6I CTCTTC 1 cut(s) 2100
BstACI GRCGYC 2 cut(s) 70, 736
BstAPI GCANNNNNTGC 2 cut(s) 201, 261
BstBAI YACGTR 1 cut(s) 466
BstC8I GCNNGC 5 cut(s) 121, 366, 1380, 2041, 2165
BstDEI CTNAG 7 cut(s) 180, 696, 1779, 1848, 1963, 2205, 2260
BstDSI CCRYGG 2 cut(s) 1622, 1977
BstENI CCTNNNNNAGG 1 cut(s) 948
BstF5I GGATG 4 cut(s) 1753, 1805, 1835, 1982
BstKTI GATC 6 cut(s) 59, 397, 670, 1703, 1786, 2239
BstMAI GTCTC 3 cut(s) 430, 438, 1025
BstMBI GATC 6 cut(s) 56, 394, 667, 1700, 1783, 2236
BstNI CCWGG 2 cut(s) 1455, 1827
BstNSI RCATGY 2 cut(s) 461, 1640
BstSCI CCNGG 6 cut(s) 625, 658, 1295, 1453, 1508, 1825
BstSFI CTRYAG 4 cut(s) 262, 1311, 1359, 2292
BstSLI GKGCMC 2 cut(s) 1774, 2073
BstV1I GCAGC 5 cut(s) 1540, 1549, 1660, 2023, 2200
BstX2I RGATCY 4 cut(s) 56, 394, 1700, 1783
BstXI CCANNNNNNTGG 1 cut(s) 991
BstYI RGATCY 4 cut(s) 56, 394, 1700, 1783
Bsu36I CCTNAGG 2 cut(s) 1779, 1848
BsuI GTATCC 1 cut(s) 796
BsuRI GGCC 6 cut(s) 165, 314, 943, 1166, 1519, 2071
BtgI CCRYGG 2 cut(s) 1622, 1977
BtgZI GCGATG 1 cut(s) 309
BtsCI GGATG 4 cut(s) 1753, 1805, 1835, 1982
BtsI GCAGTG 2 cut(s) 271, 1878
BtsIMutI CAGTG 5 cut(s) 271, 309, 1156, 1342, 1878
BveI ACCTGC 2 cut(s) 1081, 2275
Cac8I GCNNGC 5 cut(s) 121, 366, 1380, 2041, 2165
CaiI CAGNNNCTG 3 cut(s) 1262, 1826, 2267
Cfr10I RCCGGY 1 cut(s) 1502
Cfr13I GGNCC 8 cut(s) 215, 618, 1164, 1604, 1841, 1943, 2069, 2070
CseI GACGC 2 cut(s) 725, 818
Csp6I GTAC 8 cut(s) 463, 863, 1392, 1585, 1719, 1994, 2034, 2102
CspAI ACCGGT 1 cut(s) 1502
CspCI CAANNNNNGTGG 2 cut(s) 247, 282
CviAII CATG 6 cut(s) 458, 1321, 1623, 1637, 1891, 1978
CviQI GTAC 8 cut(s) 463, 863, 1392, 1585, 1719, 1994, 2034, 2102
DdeI CTNAG 7 cut(s) 180, 696, 1779, 1848, 1963, 2205, 2260
DpnI GATC 6 cut(s) 58, 396, 669, 1702, 1785, 2238
DpnII GATC 6 cut(s) 56, 394, 667, 1700, 1783, 2236
DraIII CACNNNGTG 1 cut(s) 1151
EaeI YGGCCR 1 cut(s) 312
Eam1104I CTCTTC 1 cut(s) 2100
EarI CTCTTC 1 cut(s) 2100
Eco130I CCWWGG 3 cut(s) 1622, 1977, 2044
Eco147I AGGCCT 2 cut(s) 165, 943
Eco24I GRGCYC 1 cut(s) 2073
Eco47I GGWCC 5 cut(s) 215, 618, 1604, 1841, 1943
Eco57I CTGAAG 2 cut(s) 969, 2157
Eco81I CCTNAGG 2 cut(s) 1779, 1848
EcoNI CCTNNNNNAGG 1 cut(s) 948
EcoO109I RGGNCCY 2 cut(s) 2069, 2070
EcoRII CCWGG 2 cut(s) 1453, 1825
EcoT14I CCWWGG 3 cut(s) 1622, 1977, 2044
EcoT22I ATGCAT 2 cut(s) 197, 2116
EcoT38I GRGCYC 1 cut(s) 2073
ErhI CCWWGG 3 cut(s) 1622, 1977, 2044
FaeI CATG 6 cut(s) 461, 1324, 1626, 1640, 1894, 1981
FaqI GGGAC 6 cut(s) 228, 609, 642, 837, 1250, 2062
FatI CATG 6 cut(s) 457, 1320, 1622, 1636, 1890, 1977
FauI CCCGC 1 cut(s) 1387
FauNDI CATATG 2 cut(s) 589, 2110
FblI GTMKAC 1 cut(s) 679
Fnu4HI GCNGC 5 cut(s) 1529, 1538, 1674, 2012, 2214
FokI GGATG 4 cut(s) 1760, 1792, 1842, 1969
FriOI GRGCYC 1 cut(s) 2073
Fsp4HI GCNGC 5 cut(s) 1529, 1538, 1674, 2012, 2214
FspBI CTAG 8 cut(s) 80, 557, 671, 1269, 1485, 1521, 1662, 2040
GluI GCNGC 5 cut(s) 1529, 1538, 1674, 2012, 2214
GsaI CCCAGC 1 cut(s) 1746
GsuI CTGGAG 3 cut(s) 1068, 1425, 1935
HaeIII GGCC 6 cut(s) 165, 314, 943, 1166, 1519, 2071
HapII CCGG 6 cut(s) 91, 627, 660, 1296, 1503, 1509
HgaI GACGC 2 cut(s) 725, 818
Hin1I GRCGYC 2 cut(s) 70, 736
Hin1II CATG 6 cut(s) 461, 1324, 1626, 1640, 1894, 1981
HincII GTYRAC 3 cut(s) 680, 1419, 1658
HindII GTYRAC 3 cut(s) 680, 1419, 1658
HinfI GANTC 7 cut(s) 184, 536, 560, 692, 797, 1229, 2256
HpaII CCGG 6 cut(s) 91, 627, 660, 1296, 1503, 1509
HphI GGTGA 4 cut(s) 497, 526, 536, 701
Hpy166II GTNNAC 9 cut(s) 285, 321, 463, 680, 1419, 1604, 1658, 1772, 1943
Hpy188I TCNGA 4 cut(s) 510, 790, 1243, 2157
Hpy8I GTNNAC 9 cut(s) 285, 321, 463, 680, 1419, 1604, 1658, 1772, 1943
Hpy99I CGWCG 4 cut(s) 72, 75, 606, 741
HpyCH4III ACNGT 7 cut(s) 222, 684, 779, 1151, 1396, 1584, 1723
HpyCH4IV ACGT 4 cut(s) 70, 403, 465, 601
HpyF3I CTNAG 7 cut(s) 180, 696, 1779, 1848, 1963, 2205, 2260
HpySE526I ACGT 4 cut(s) 70, 403, 465, 601
Hsp92I GRCGYC 2 cut(s) 70, 736
Hsp92II CATG 6 cut(s) 461, 1324, 1626, 1640, 1894, 1981
Kzo9I GATC 6 cut(s) 56, 394, 667, 1700, 1783, 2236
LmnI GCTCC 8 cut(s) 93, 646, 1081, 1186, 1210, 1444, 2186, 2210
Lsp1109I GCAGC 5 cut(s) 1540, 1549, 1660, 2023, 2200
LweI GCATC 6 cut(s) 182, 1048, 1540, 1736, 1991, 2123
MaeI CTAG 8 cut(s) 80, 557, 671, 1269, 1485, 1521, 1662, 2040
MaeII ACGT 4 cut(s) 70, 403, 465, 601
MaeIII GTNAC 4 cut(s) 524, 1114, 1151, 1316
MalI GATC 6 cut(s) 58, 396, 669, 1702, 1785, 2238
MboI GATC 6 cut(s) 56, 394, 667, 1700, 1783, 2236
MboII GAAGA 6 cut(s) 91, 486, 755, 972, 1776, 2087
MfeI CAATTG 1 cut(s) 1758
MflI RGATCY 4 cut(s) 56, 394, 1700, 1783
MhlI GDGCHC 3 cut(s) 1441, 1774, 2073
MlsI TGGCCA 1 cut(s) 314
MluNI TGGCCA 1 cut(s) 314
MlyI GAGTC 1 cut(s) 530
MmeI TCCRAC 1 cut(s) 1103
Mox20I TGGCCA 1 cut(s) 314
Mph1103I ATGCAT 2 cut(s) 197, 2116
MroXI GAANNNNTTC 2 cut(s) 706, 976
MscI TGGCCA 1 cut(s) 314
MseI TTAA 6 cut(s) 171, 198, 336, 453, 1046, 2228
MslI CAYNNNNRTG 1 cut(s) 1883
Msp20I TGGCCA 1 cut(s) 314
MspA1I CMGCKG 1 cut(s) 1673
MspI CCGG 6 cut(s) 91, 627, 660, 1296, 1503, 1509
MspR9I CCNGG 6 cut(s) 627, 660, 1297, 1455, 1510, 1827
MunI CAATTG 1 cut(s) 1758
MvaI CCWGG 2 cut(s) 1455, 1827
NciI CCSGG 4 cut(s) 627, 660, 1297, 1510
NcoI CCATGG 2 cut(s) 1622, 1977
NdeI CATATG 2 cut(s) 589, 2110
NdeII GATC 6 cut(s) 56, 394, 667, 1700, 1783, 2236
NheI GCTAGC 1 cut(s) 2039
NlaIII CATG 6 cut(s) 461, 1324, 1626, 1640, 1894, 1981
NlaIV GGNNCC 7 cut(s) 216, 997, 1010, 1282, 1507, 2070, 2071
NmeAIII GCCGAG 1 cut(s) 149
NmuCI GTSAC 2 cut(s) 524, 1151
NsiI ATGCAT 2 cut(s) 197, 2116
NspI RCATGY 2 cut(s) 461, 1640
PaqCI CACCTGC 1 cut(s) 2275
PceI AGGCCT 2 cut(s) 165, 943
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PdmI GAANNNNTTC 2 cut(s) 706, 976
PfeI GAWTC 6 cut(s) 184, 560, 692, 797, 1229, 2256
PflMI CCANNNNNTGG 3 cut(s) 1337, 1598, 1826
PfoI TCCNGGA 2 cut(s) 658, 1825
PinAI ACCGGT 1 cut(s) 1502
PkrI GCNGC 5 cut(s) 1530, 1539, 1675, 2013, 2215
PleI GAGTC 1 cut(s) 530
PpsI GAGTC 1 cut(s) 530
Ppu21I YACGTR 1 cut(s) 466
Psp6I CCWGG 2 cut(s) 1453, 1825
PspFI CCCAGC 1 cut(s) 1742
PspGI CCWGG 2 cut(s) 1453, 1825
PspN4I GGNNCC 7 cut(s) 216, 997, 1010, 1282, 1507, 2070, 2071
PspOMI GGGCCC 1 cut(s) 2069
PspPI GGNCC 8 cut(s) 215, 618, 1164, 1604, 1841, 1943, 2069, 2070
PsrI GAACNNNNNNTAC 2 cut(s) 979, 1011
PstI CTGCAG 2 cut(s) 266, 2296
PstNI CAGNNNCTG 3 cut(s) 1262, 1826, 2267
PsuI RGATCY 4 cut(s) 56, 394, 1700, 1783
PvuII CAGCTG 1 cut(s) 1673
RsaI GTAC 8 cut(s) 464, 864, 1393, 1586, 1720, 1995, 2035, 2103
RsaNI GTAC 8 cut(s) 463, 863, 1392, 1585, 1719, 1994, 2034, 2102
RseI CAYNNNNRTG 1 cut(s) 1883
SalI GTCGAC 1 cut(s) 678
SaqAI TTAA 6 cut(s) 171, 198, 336, 453, 1046, 2228
SatI GCNGC 5 cut(s) 1529, 1538, 1674, 2012, 2214
Sau3AI GATC 6 cut(s) 56, 394, 667, 1700, 1783, 2236
Sau96I GGNCC 8 cut(s) 215, 618, 1164, 1604, 1841, 1943, 2069, 2070
ScaI AGTACT 1 cut(s) 1720
SchI GAGTC 1 cut(s) 530
ScrFI CCNGG 6 cut(s) 627, 660, 1297, 1455, 1510, 1827
SduI GDGCHC 3 cut(s) 1441, 1774, 2073
SfaNI GCATC 6 cut(s) 182, 1048, 1540, 1736, 1991, 2123
SfcI CTRYAG 4 cut(s) 262, 1311, 1359, 2292
SinI GGWCC 5 cut(s) 215, 618, 1604, 1841, 1943
SmiMI CAYNNNNRTG 1 cut(s) 1883
SmlI CTYRAG 2 cut(s) 1095, 1200
SmoI CTYRAG 2 cut(s) 1095, 1200
SseBI AGGCCT 2 cut(s) 165, 943
SsiI CCGC 6 cut(s) 62, 86, 630, 1284, 1380, 1997
SspMI CTAG 8 cut(s) 80, 557, 671, 1269, 1485, 1521, 1662, 2040
StuI AGGCCT 2 cut(s) 165, 943
StyD4I CCNGG 6 cut(s) 625, 658, 1295, 1453, 1508, 1825
StyI CCWWGG 3 cut(s) 1622, 1977, 2044
TaaI ACNGT 7 cut(s) 222, 684, 779, 1151, 1396, 1584, 1723
TaiI ACGT 4 cut(s) 73, 406, 468, 604
TaqI TCGA 6 cut(s) 102, 160, 442, 577, 679, 739
TatI WGTACW 2 cut(s) 1584, 1718
TfiI GAWTC 6 cut(s) 184, 560, 692, 797, 1229, 2256
Tru1I TTAA 6 cut(s) 171, 198, 336, 453, 1046, 2228
Tru9I TTAA 6 cut(s) 171, 198, 336, 453, 1046, 2228
TscAI CASTG 5 cut(s) 271, 309, 1156, 1342, 1885
TseFI GTSAC 2 cut(s) 524, 1151
TseI GCWGC 5 cut(s) 1528, 1537, 1673, 2011, 2213
Tsp45I GTSAC 2 cut(s) 524, 1151
TspDTI ATGAA 8 cut(s) 162, 552, 558, 612, 1794, 1811, 2070, 2214
TspGWI ACGGA 2 cut(s) 166, 896
TspRI CASTG 5 cut(s) 271, 309, 1156, 1342, 1885
Van91I CCANNNNNTGG 3 cut(s) 1337, 1598, 1826
VneI GTGCAC 1 cut(s) 1770
VpaK11BI GGWCC 5 cut(s) 215, 618, 1604, 1841, 1943
XagI CCTNNNNNAGG 1 cut(s) 948
XapI RAATTY 6 cut(s) 235, 1141, 1219, 1627, 1735, 2151
XbaI TCTAGA 1 cut(s) 670
XceI RCATGY 2 cut(s) 461, 1640
XcmI CCANNNNNNNNNTGG 3 cut(s) 856, 1827, 2061
XmiI GTMKAC 1 cut(s) 679
XmnI GAANNNNTTC 2 cut(s) 706, 976
XspI CTAG 8 cut(s) 80, 557, 671, 1269, 1485, 1521, 1662, 2040
ZraI GACGTC 1 cut(s) 71
ZrmI AGTACT 1 cut(s) 1720
Zsp2I ATGCAT 2 cut(s) 197, 2116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.