Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0010854

Physical Location & Seq

Genomic Coordinates Reverse (-)

15217758 .. 15221062

Gene Structure 3305 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1193100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1506 bp

ATGGAGGATGATAATGGACTTGAACTTAGTTTGGGTCTCTCTTGGGGTGGATCATCTGCCAAAACCAAGGGTAAAAATGGTGCTTCCCCAAATGCTAGAGCAGAAGAAGGTGATAGAGGCAACAAACTAGCGGATGATTTTAAGGACTTTCTTCATGGTGGGGCTCAAAAACAAGAATCTAGTAGCAGCTCTCAGAGAAGTGATTCAGTGAAACCTAAGGAAAACTTCTTCAATGACCTTTCCAAGGCCAGTGTTGATGCAGATGCATCAATGAACTTGAACGGTAGAGGAGTCTGGGTTCCAAACAATAACAAGTCTGATGAAACAGAGGAGGAAAAACGGACTGAGGCTAGTAATAAGCGCAAGAATTTGTTTGACGAGATGAACCATCAAAAGAAGCATGAGAGAGAAACTCATTACTCTGATATGCATGACAAGACGAGAGCATCACACATTTCCGTTACAGAAGATGGCTCAACTGCTGAAAATGAAGACGTGGCAGATTCTGAAGTGGAGGGTTCATCCTCAGTGCCGATTTCTCAACATGCTGAGGGCGTCAAGCGATTTGTTGGATCCGGTGACTCTTCTGATGCTCAGAAGGAAGTTCGAAGGTTTGCTGATTCCAATGTTGTCGACCTAAATGGGCAGAAGAGGTTTAATATCTCATCAGAAAATGAATATAAGCTTGGGAATATGGCGTATGGCAGTCCCTTCTCACTGCAATCAGTAAACATGAACATACCTTATTCTCTCCCGACGAAGGAATCTAATTCAGTTGGTGCGACCACCACATCAGGACATCCAATGCATGGGATGATGCAGGCGATGCCTACTGTTACTGGTGAGCGAACAGGGACTCAACCTGTGAATCCTGGAAACTTGCCAGTTATGTTTGGCTATTCACCTGTCCAGCTCCCAATGTTGGATAAAGATAATTCCTGGGGTGCGATTCCTCATGCTCAACAATTCCATCCTGCCTATGCTGGCAGAAATCCACCAAACTCAGCTATGATGCAAGTCGTTTCACATAATTCACCTGATGTTGCACAATATGATGGGAGGATGTTAGAACGGGCGAGAGGTGATGGCAAACAGAATGTCACTGAAGAGGGCGCATCTTCTCAGGTAGAAGAAGACACAAAAGTAAGTAGCGCAAACCTCGGGATGAAAGATGCACCTGGCCGCCCAACGGCAGAGGACTTATCTCTTGATTTTTCAGCTATAAAGCCAGGTATTGCTGCAGACATAAAATTTGGGGGATCTGGTTCATGCCCGAACCTACCATGGGTTTCTACCAAGGGCCAAGGCCCAAATGGTAAGACAATTTCTGGTGTAACTTACCGATACAGCACAAACCAAATCAAGATAGTTTGTGCTTGCCACGGCTTGCACATGTCCCCTGAAGAGTTTGTTCGTCATGCAAGCGACGAGCCATCTAATCCCGAAAGTGGCACTGGTTTGGCAGCATTTCCTAATGGCAATCCTTCTGCCTCGGCTCATACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032870 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EAR Jas

Protein Analysis

502

Amino Acids

53.83

Weight (kDa)

5.43

Isoelectric Point (pI)

44.21

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010854)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28910 AT4G28910 AT4G28910
fragaria_vesca	FvH4_1g05760 FvH4_1g05760 FvH4_1g05760
malus_domestica	MD02G1058800.v1.1 MD15G1193100.v1.1
prunus_persica	Prupe.7G224200_v2.0.a1
pyrus_communis	pycom02g04760 pycom15g17290
rosa_chinensis	RchiOBHm_Chr2g0091401
rosa_laevigata	RLG00000016221
rosa_roxburghii	Rroxscaffold_2G00150110
rosa_rugosa	Rorug02G0017500
rosa_samantha	Rh2AG062500 Rh2BG061500 Rh2CG063600 Rh2DG062100
rosa_wichuraiana	Rw2G005770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	809
AccI	GTMKAC	1 cut(s)	633
AciI	CCGC	2 cut(s)	131, 1183
AclWI	GGATC	4 cut(s)	58, 567, 580, 1267
AcoI	YGGCCR	1 cut(s)	1180
AcsI	RAATTY	2 cut(s)	367, 1250
AcuI	CTGAAG	3 cut(s)	528, 1125, 1422
AcyI	GRCGYC	1 cut(s)	555
AfiI	CCNNNNNNNGG	7 cut(s)	244, 760, 809, 922, 1189, 1285, 1448
AflIII	ACRYGT	1 cut(s)	1392
AgsI	TTSAA	3 cut(s)	23, 232, 280
AjiI	CACGTC	1 cut(s)	496
AjnI	CCWGG	4 cut(s)	871, 938, 1177, 1228
AjuI	GAANNNNNNNTTGG	2 cut(s)	669, 701
AloI	GAACNNNNNNTCC	2 cut(s)	282, 314
AluBI	AGCT	5 cut(s)	189, 685, 913, 1007, 1220
AluI	AGCT	5 cut(s)	189, 685, 913, 1007, 1220
Alw26I	GTCTC	1 cut(s)	41
AlwI	GGATC	4 cut(s)	58, 567, 580, 1267
AlwNI	CAGNNNCTG	1 cut(s)	506
Ama87I	CYCGRG	1 cut(s)	1160
AoxI	GGCC	4 cut(s)	246, 1180, 1300, 1306
ApeKI	GCWGC	3 cut(s)	186, 1238, 1463
ApoI	RAATTY	2 cut(s)	367, 1250
ArsI	GACNNNNNNTTYG	4 cut(s)	1083, 1115, 1235, 1267
Asp700I	GAANNNNTTC	1 cut(s)	202
AspLEI	GCGC	3 cut(s)	363, 1115, 1154
AspS9I	GGNCC	2 cut(s)	1300, 1307
AsuHPI	GGTGA	6 cut(s)	122, 590, 854, 894, 1026, 1094
AsuII	TTCGAA	1 cut(s)	607
AvaI	CYCGRG	1 cut(s)	1160
AxyI	CCTNAGG	1 cut(s)	216
BamHI	GGATCC	1 cut(s)	572
BanII	GRGCYC	1 cut(s)	166
BbsI	GAAGAC	2 cut(s)	498, 1140
BbvCI	CCTCAGC	1 cut(s)	549
BbvI	GCAGC	3 cut(s)	198, 1225, 1475
BccI	CCATC	6 cut(s)	396, 464, 978, 1049, 1079, 1441
BceAI	ACGGC	2 cut(s)	1206, 1399
BciT130I	CCWGG	4 cut(s)	873, 940, 1179, 1230
BcoDI	GTCTC	1 cut(s)	41
BfaI	CTAG	4 cut(s)	96, 128, 180, 351
BfmI	CTRYAG	1 cut(s)	1239
BisI	GCNGC	4 cut(s)	187, 1183, 1239, 1464
BlsI	GCNGC	4 cut(s)	188, 1184, 1240, 1465
Bme1390I	CCNGG	4 cut(s)	873, 940, 1179, 1230
BmeT110I	CYCGRG	1 cut(s)	1160
BmgBI	CACGTC	1 cut(s)	496
BmgT120I	GGNCC	2 cut(s)	1300, 1307
BmiI	GGNNCC	2 cut(s)	300, 574
BmrFI	CCNGG	4 cut(s)	873, 940, 1179, 1230
BpiI	GAAGAC	2 cut(s)	498, 1140
BplI	GAGNNNNNCTC	2 cut(s)	397, 429
Bpu10I	CCTNAGC	1 cut(s)	549
Bpu14I	TTCGAA	1 cut(s)	607
BsaHI	GRCGYC	1 cut(s)	555
BsaI	GGTCTC	1 cut(s)	41
BsaJI	CCNNGG	9 cut(s)	66, 243, 939, 1159, 1283, 1296, 1303, 1381, 1491
BsaWI	WCCGGW	1 cut(s)	575
BsaXI	ACNNNNNCTCC	2 cut(s)	282, 312
Bsc4I	CCNNNNNNNGG	7 cut(s)	244, 760, 809, 922, 1189, 1285, 1448
Bse1I	ACTGG	4 cut(s)	249, 844, 884, 1459
Bse21I	CCTNAGG	1 cut(s)	216
BseBI	CCWGG	4 cut(s)	873, 940, 1179, 1230
BseDI	CCNNGG	9 cut(s)	66, 243, 939, 1159, 1283, 1296, 1303, 1381, 1491
BseGI	GGATG	8 cut(s)	13, 139, 521, 799, 819, 970, 1068, 1170
BseLI	CCNNNNNNNGG	7 cut(s)	244, 760, 809, 922, 1189, 1285, 1448
BseMII	CTCAG	7 cut(s)	206, 336, 540, 540, 608, 1017, 1136
BseNI	ACTGG	4 cut(s)	249, 844, 884, 1459
BseRI	GAGGAG	2 cut(s)	303, 344
BseXI	GCAGC	3 cut(s)	198, 1225, 1475
BshFI	GGCC	4 cut(s)	248, 1182, 1302, 1308
BsiHKCI	CYCGRG	1 cut(s)	1160
BsiSI	CCGG	1 cut(s)	576
BslFI	GGGAC	3 cut(s)	693, 868, 1381
BslI	CCNNNNNNNGG	7 cut(s)	244, 760, 809, 922, 1189, 1285, 1448
BsmAI	GTCTC	1 cut(s)	41
BsmFI	GGGAC	3 cut(s)	693, 868, 1381
BsnI	GGCC	4 cut(s)	248, 1182, 1302, 1308
Bso31I	GGTCTC	1 cut(s)	41
BsoBI	CYCGRG	1 cut(s)	1160
Bsp119I	TTCGAA	1 cut(s)	607
Bsp1286I	GDGCHC	1 cut(s)	166
Bsp143I	GATC	3 cut(s)	50, 572, 1259
Bsp19I	CCATGG	1 cut(s)	1283
BspACI	CCGC	2 cut(s)	131, 1183
BspANI	GGCC	4 cut(s)	248, 1182, 1302, 1308
BspCNI	CTCAG	7 cut(s)	205, 337, 539, 541, 607, 1016, 1135
BspLI	GGNNCC	2 cut(s)	300, 574
BspMAI	CTGCAG	1 cut(s)	1243
BspPI	GGATC	4 cut(s)	58, 567, 580, 1267
BspT104I	TTCGAA	1 cut(s)	607
BspTNI	GGTCTC	1 cut(s)	41
BsrI	ACTGG	4 cut(s)	249, 844, 884, 1459
BssECI	CCNNGG	9 cut(s)	66, 243, 939, 1159, 1283, 1296, 1303, 1381, 1491
BssMI	GATC	3 cut(s)	50, 572, 1259
BssNI	GRCGYC	1 cut(s)	555
BssT1I	CCWWGG	5 cut(s)	66, 243, 1283, 1296, 1303
Bst2UI	CCWGG	4 cut(s)	873, 940, 1179, 1230
Bst4CI	ACNGT	2 cut(s)	284, 835
Bst6I	CTCTTC	4 cut(s)	589, 644, 1101, 1398
BstACI	GRCGYC	1 cut(s)	555
BstAPI	GCANNNNNTGC	1 cut(s)	826
BstBI	TTCGAA	1 cut(s)	607
BstC8I	GCNNGC	5 cut(s)	822, 985, 1378, 1388, 1423
BstDEI	CTNAG	9 cut(s)	26, 192, 216, 345, 526, 549, 594, 1003, 1122
BstDSI	CCRYGG	2 cut(s)	1283, 1381
BstENI	CCTNNNNNAGG	1 cut(s)	242
BstF5I	GGATG	8 cut(s)	13, 139, 521, 799, 819, 970, 1068, 1170
BstHHI	GCGC	3 cut(s)	363, 1115, 1154
BstKTI	GATC	3 cut(s)	53, 575, 1262
BstMAI	GTCTC	1 cut(s)	41
BstMBI	GATC	3 cut(s)	50, 572, 1259
BstMWI	GCNNNNNNNGC	1 cut(s)	826
BstNI	CCWGG	4 cut(s)	873, 940, 1179, 1230
BstNSI	RCATGY	2 cut(s)	548, 1396
BstSCI	CCNGG	4 cut(s)	871, 938, 1177, 1228
BstSFI	CTRYAG	1 cut(s)	1239
BstV1I	GCAGC	3 cut(s)	198, 1225, 1475
BstV2I	GAAGAC	2 cut(s)	498, 1140
BstX2I	RGATCY	2 cut(s)	572, 1259
BstYI	RGATCY	2 cut(s)	572, 1259
Bsu36I	CCTNAGG	1 cut(s)	216
BsuRI	GGCC	4 cut(s)	248, 1182, 1302, 1308
BtgI	CCRYGG	2 cut(s)	1283, 1381
BtgZI	GCGATG	1 cut(s)	839
BtrI	CACGTC	1 cut(s)	496
BtsCI	GGATG	8 cut(s)	13, 139, 521, 799, 819, 970, 1068, 1170
BtsI	GCAGTG	1 cut(s)	716
BtsIMutI	CAGTG	6 cut(s)	213, 256, 534, 716, 1101, 1452
Cac8I	GCNNGC	5 cut(s)	822, 985, 1378, 1388, 1423
CaiI	CAGNNNCTG	1 cut(s)	506
CfoI	GCGC	3 cut(s)	363, 1115, 1154
Cfr13I	GGNCC	2 cut(s)	1300, 1307
CseI	GACGC	1 cut(s)	544
DdeI	CTNAG	9 cut(s)	26, 192, 216, 345, 526, 549, 594, 1003, 1122
DpnI	GATC	3 cut(s)	52, 574, 1261
DpnII	GATC	3 cut(s)	50, 572, 1259
EaeI	YGGCCR	1 cut(s)	1180
Eam1104I	CTCTTC	4 cut(s)	589, 644, 1101, 1398
EarI	CTCTTC	4 cut(s)	589, 644, 1101, 1398
Eco130I	CCWWGG	5 cut(s)	66, 243, 1283, 1296, 1303
Eco24I	GRGCYC	1 cut(s)	166
Eco31I	GGTCTC	1 cut(s)	41
Eco57I	CTGAAG	3 cut(s)	528, 1125, 1422
Eco81I	CCTNAGG	1 cut(s)	216
Eco88I	CYCGRG	1 cut(s)	1160
EcoNI	CCTNNNNNAGG	1 cut(s)	242
EcoRII	CCWGG	4 cut(s)	871, 938, 1177, 1228
EcoT14I	CCWWGG	5 cut(s)	66, 243, 1283, 1296, 1303
EcoT22I	ATGCAT	3 cut(s)	268, 432, 810
EcoT38I	GRGCYC	1 cut(s)	166
ErhI	CCWWGG	5 cut(s)	66, 243, 1283, 1296, 1303
FalI	AAGNNNNNCTT	2 cut(s)	209, 241
FaqI	GGGAC	3 cut(s)	693, 868, 1381
FblI	GTMKAC	1 cut(s)	633
Fnu4HI	GCNGC	4 cut(s)	187, 1183, 1239, 1464
FokI	GGATG	8 cut(s)	20, 146, 508, 786, 826, 957, 1075, 1177
FriOI	GRGCYC	1 cut(s)	166
Fsp4HI	GCNGC	4 cut(s)	187, 1183, 1239, 1464
FspBI	CTAG	4 cut(s)	96, 128, 180, 351
GlaI	GCGC	3 cut(s)	362, 1114, 1153
GluI	GCNGC	4 cut(s)	187, 1183, 1239, 1464
HaeIII	GGCC	4 cut(s)	248, 1182, 1302, 1308
HapII	CCGG	1 cut(s)	576
HgaI	GACGC	1 cut(s)	544
HhaI	GCGC	3 cut(s)	363, 1115, 1154
Hin1I	GRCGYC	1 cut(s)	555
Hin6I	GCGC	3 cut(s)	361, 1113, 1152
HinP1I	GCGC	3 cut(s)	361, 1113, 1152
HincII	GTYRAC	1 cut(s)	634
HindII	GTYRAC	1 cut(s)	634
HindIII	AAGCTT	1 cut(s)	683
HpaII	CCGG	1 cut(s)	576
HphI	GGTGA	6 cut(s)	122, 590, 854, 894, 1026, 1094
Hpy166II	GTNNAC	2 cut(s)	634, 730
Hpy188I	TCNGA	7 cut(s)	195, 319, 424, 508, 589, 597, 670
Hpy188III	TCNNGA	6 cut(s)	754, 795, 1162, 1208, 1363, 1442
Hpy8I	GTNNAC	2 cut(s)	634, 730
Hpy99I	CGWCG	2 cut(s)	760, 1430
HpyAV	CCTTC	6 cut(s)	101, 592, 603, 721, 754, 1494
HpyCH4III	ACNGT	2 cut(s)	284, 835
HpyCH4IV	ACGT	1 cut(s)	495
HpyF10VI	GCNNNNNNNGC	1 cut(s)	826
HpyF3I	CTNAG	9 cut(s)	26, 192, 216, 345, 526, 549, 594, 1003, 1122
HpySE526I	ACGT	1 cut(s)	495
Hsp92I	GRCGYC	1 cut(s)	555
HspAI	GCGC	3 cut(s)	361, 1113, 1152
Kzo9I	GATC	3 cut(s)	50, 572, 1259
LmnI	GCTCC	1 cut(s)	918
Lsp1109I	GCAGC	3 cut(s)	198, 1225, 1475
MaeI	CTAG	4 cut(s)	96, 128, 180, 351
MaeII	ACGT	1 cut(s)	495
MaeIII	GTNAC	5 cut(s)	460, 578, 835, 1099, 1333
MalI	GATC	3 cut(s)	52, 574, 1261
MboI	GATC	3 cut(s)	50, 572, 1259
MflI	RGATCY	2 cut(s)	572, 1259
MhlI	GDGCHC	1 cut(s)	166
MluCI	AATT	7 cut(s)	367, 769, 934, 965, 1030, 1250, 1323
MlyI	GAGTC	3 cut(s)	300, 575, 850
MmeI	TCCRAC	2 cut(s)	550, 903
Mph1103I	ATGCAT	3 cut(s)	268, 432, 810
MroXI	GAANNNNTTC	1 cut(s)	202
MseI	TTAA	2 cut(s)	141, 657
MspI	CCGG	1 cut(s)	576
MspR9I	CCNGG	4 cut(s)	873, 940, 1179, 1230
MvaI	CCWGG	4 cut(s)	873, 940, 1179, 1230
MwoI	GCNNNNNNNGC	1 cut(s)	826
NcoI	CCATGG	1 cut(s)	1283
NdeII	GATC	3 cut(s)	50, 572, 1259
NlaIV	GGNNCC	2 cut(s)	300, 574
NmeAIII	GCCGAG	1 cut(s)	1472
NmuCI	GTSAC	2 cut(s)	578, 1099
NsiI	ATGCAT	3 cut(s)	268, 432, 810
NspI	RCATGY	2 cut(s)	548, 1396
NspV	TTCGAA	1 cut(s)	607
PciI	ACATGT	1 cut(s)	1392
PdmI	GAANNNNTTC	1 cut(s)	202
PfeI	GAWTC	7 cut(s)	176, 203, 503, 620, 764, 868, 949
PflMI	CCANNNNNTGG	1 cut(s)	809
PfoI	TCCNGGA	1 cut(s)	871
PkrI	GCNGC	4 cut(s)	188, 1184, 1240, 1465
PleI	GAGTC	3 cut(s)	299, 575, 850
PpsI	GAGTC	3 cut(s)	299, 575, 850
PscI	ACATGT	1 cut(s)	1392
Psp6I	CCWGG	4 cut(s)	871, 938, 1177, 1228
PspGI	CCWGG	4 cut(s)	871, 938, 1177, 1228
PspN4I	GGNNCC	2 cut(s)	300, 574
PspPI	GGNCC	2 cut(s)	1300, 1307
PstI	CTGCAG	1 cut(s)	1243
PstNI	CAGNNNCTG	1 cut(s)	506
PsuI	RGATCY	2 cut(s)	572, 1259
SalI	GTCGAC	1 cut(s)	632
SaqAI	TTAA	2 cut(s)	141, 657
SatI	GCNGC	4 cut(s)	187, 1183, 1239, 1464
Sau3AI	GATC	3 cut(s)	50, 572, 1259
Sau96I	GGNCC	2 cut(s)	1300, 1307
SchI	GAGTC	3 cut(s)	300, 575, 850
ScrFI	CCNGG	4 cut(s)	873, 940, 1179, 1230
SduI	GDGCHC	1 cut(s)	166
SfcI	CTRYAG	1 cut(s)	1239
SfuI	TTCGAA	1 cut(s)	607
Sse9I	AATT	7 cut(s)	367, 769, 934, 965, 1030, 1250, 1323
SsiI	CCGC	2 cut(s)	131, 1183
SspMI	CTAG	4 cut(s)	96, 128, 180, 351
StyD4I	CCNGG	4 cut(s)	871, 938, 1177, 1228
StyI	CCWWGG	5 cut(s)	66, 243, 1283, 1296, 1303
TaaI	ACNGT	2 cut(s)	284, 835
TaiI	ACGT	1 cut(s)	498
TaqI	TCGA	2 cut(s)	607, 633
TasI	AATT	7 cut(s)	367, 769, 934, 965, 1030, 1250, 1323
TauI	GCSGC	1 cut(s)	1185
TfiI	GAWTC	7 cut(s)	176, 203, 503, 620, 764, 868, 949
Tru1I	TTAA	2 cut(s)	141, 657
Tru9I	TTAA	2 cut(s)	141, 657
TscAI	CASTG	6 cut(s)	213, 256, 534, 723, 1108, 1459
TseFI	GTSAC	2 cut(s)	578, 1099
TseI	GCWGC	3 cut(s)	186, 1238, 1463
Tsp45I	GTSAC	2 cut(s)	578, 1099
TspGWI	ACGGA	2 cut(s)	355, 448
TspRI	CASTG	6 cut(s)	213, 256, 534, 723, 1108, 1459
Van91I	CCANNNNNTGG	1 cut(s)	809
XagI	CCTNNNNNAGG	1 cut(s)	242
XapI	RAATTY	2 cut(s)	367, 1250
XceI	RCATGY	2 cut(s)	548, 1396
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1310
XmiI	GTMKAC	1 cut(s)	633
XmnI	GAANNNNTTC	1 cut(s)	202
XspI	CTAG	4 cut(s)	96, 128, 180, 351
Zsp2I	ATGCAT	3 cut(s)	268, 432, 810

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.