Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0010854

Physical Location & Seq

Genomic Coordinates Reverse (-)

12179843 .. 12181596

Gene Structure 1754 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom15g17290.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1509 bp

ATGGAGGATGATAATGGACTTGAACTTAGTTTGGGTCTCTCTTGGGGTGGATCATCTGCCAAAACCAAGGGTAAAAATGGTGCTTCCCCAAATGCTAGAGCAGAAGAAGGAGATAGAGGCAACAAACTAGCGGATGATTTTAAGGACTTTCTTCATGGTGCGGCTCAAAAACAAGAATCTAGTAGTAGCTCTCAAAGAAGTGATTCAGTGAAACCTAAGGAAAACTTCTTCAATGACCTTTCCAAGTCCAGTGTTGATGCAGATGCATCAATGAACTTGAACGGTAGAGGAGTCTGGGTTCCAAACAATAACAAGTCTGATGAAACAGAGGAGGAAAAACGGACTGAGGCTAGTAATAAGCGCAAGAATTTGTTTGATGAGATGAACCATCAAAAGAAGCATGAGAGAGAAACTCATTACTCTGATATGCATGACAAGACGAGAGCATCACACATTTCCGTTACAGAAGATGGCTCAACTGCTGAAAATGAAGACGTGGCAGATTCTGAAGTGGAGGGTTCATCCTCAGTGCCGATTTCTCAACATGCTGAGGGCGTCAAGCGATTTGTTGGATCTGGTGACTCTTCTGATGCTCAGAAGGAAGTTCGAAGGTTTGCTGATTCCAATGTTGTCGACCTAAATGGGCAGAAGAGGTTTAATATCTCATCAGAAAATGAATATAAGCTTGGGAATATGGCGTATGGCAGTCCCTTCTCACTGCAATCAGTAAACATGATGAACATACCTTATTCTCTCCCGACGAAGGAATCTAATTCAGTTGGTGCAACCACCACATCAGGACATCCAATGCATGGGATGATGCAGTCGATGCCTACTGTTACTGGTGAACGAACAGGGACTCAACCTGTGAATCCTGGAAACTTGCCAGTTATGTTTGGCTATTCACCTGTCCAGCTCCCAATGTTGGATAAAGATAATTCCTGGGGTGCAATTCCTCATGCTCAACAATTCCATCCTGCCTATGCAGGCAGAAATCCACCAAACTCAGCTATAATGCAAGTCGTTTCACATAATTCACCTGATGTGGCACAATATGATGGGAGGATGTTAGAACGGGCGAGAGGTGATGGCAAACAGAATGTCACTGAAGAGGGCGCATCTTCTCAGGTAGAAGAAGACACAAAAGTAAGTAGCGCGAACCTCGGGATGAAAGATGCACCTGGCCGCCCAACGGCAGAGGACTTATCTCTTGATTTTTCAGCTATAAAGCCAGGTATTGCTGCAGACATAAAATTTGGGGGATCTGGTTCATGCCCGAACCTACCATGGGTTTCTACCAAAGGCCAAGGGCCAAATGGTAAGACAATTTCGGGTGTAACTTACCGATACAGCACAAACCAAATAAAGATAGTTTGTGCTTGCCACGGCTTGCACATGTCCCCTGAAGAGTTTGTTCGTCATGCAAGCGACGAGCCATCTAATCCCGAAAGTGGCACTGGTTTGGCAGCATTTCCTAATGGCAATCCTTCTGCCCCAGCTCATACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009753 GO:0009755 GO:0009867 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0019219 GO:0019222 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032870 GO:0042221 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0045892 GO:0045934 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071229 GO:0071310 GO:0071395 GO:0071495 GO:0080090 GO:1901700 GO:1901701 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EAR Jas

Protein Analysis

503

Amino Acids

54.0

Weight (kDa)

5.43

Isoelectric Point (pI)

45.92

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010854)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G28910 AT4G28910 AT4G28910
fragaria_vesca	FvH4_1g05760 FvH4_1g05760 FvH4_1g05760
malus_domestica	MD02G1058800.v1.1 MD15G1193100.v1.1
prunus_persica	Prupe.7G224200_v2.0.a1
pyrus_communis	pycom02g04760 pycom15g17290
rosa_chinensis	RchiOBHm_Chr2g0091401
rosa_laevigata	RLG00000016221
rosa_roxburghii	Rroxscaffold_2G00150110
rosa_rugosa	Rorug02G0017500
rosa_samantha	Rh2AG062500 Rh2BG061500 Rh2CG063600 Rh2DG062100
rosa_wichuraiana	Rw2G005770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	812
AccI	GTMKAC	1 cut(s)	633
AccII	CGCG	1 cut(s)	1157
AciI	CCGC	3 cut(s)	131, 161, 1186
AclWI	GGATC	3 cut(s)	58, 580, 1270
AcoI	YGGCCR	1 cut(s)	1183
AcsI	RAATTY	2 cut(s)	367, 1253
AcuI	CTGAAG	3 cut(s)	528, 1128, 1425
AcyI	GRCGYC	1 cut(s)	555
AfiI	CCNNNNNNNGG	6 cut(s)	763, 812, 925, 1192, 1288, 1451
AflIII	ACRYGT	1 cut(s)	1395
AgsI	TTSAA	3 cut(s)	23, 232, 280
AjiI	CACGTC	1 cut(s)	496
AjnI	CCWGG	4 cut(s)	874, 941, 1180, 1231
AjuI	GAANNNNNNNTTGG	2 cut(s)	669, 701
AloI	GAACNNNNNNTCC	2 cut(s)	282, 314
AluBI	AGCT	6 cut(s)	189, 685, 916, 1010, 1223, 1499
AluI	AGCT	6 cut(s)	189, 685, 916, 1010, 1223, 1499
Alw26I	GTCTC	1 cut(s)	41
AlwI	GGATC	3 cut(s)	58, 580, 1270
AlwNI	CAGNNNCTG	1 cut(s)	506
Ama87I	CYCGRG	1 cut(s)	1163
AoxI	GGCC	3 cut(s)	1183, 1303, 1310
ApeKI	GCWGC	2 cut(s)	1241, 1466
ApoI	RAATTY	2 cut(s)	367, 1253
ArsI	GACNNNNNNTTYG	4 cut(s)	1086, 1118, 1238, 1270
Asp700I	GAANNNNTTC	1 cut(s)	202
AspLEI	GCGC	3 cut(s)	363, 1118, 1157
AspS9I	GGNCC	1 cut(s)	1310
AsuHPI	GGTGA	5 cut(s)	590, 857, 897, 1029, 1097
AsuII	TTCGAA	1 cut(s)	607
AvaI	CYCGRG	1 cut(s)	1163
AxyI	CCTNAGG	1 cut(s)	216
BbsI	GAAGAC	2 cut(s)	498, 1143
BbvCI	CCTCAGC	1 cut(s)	549
BbvI	GCAGC	2 cut(s)	1228, 1478
BccI	CCATC	6 cut(s)	396, 464, 981, 1052, 1082, 1444
BceAI	ACGGC	2 cut(s)	1209, 1402
BciT130I	CCWGG	4 cut(s)	876, 943, 1182, 1233
BcoDI	GTCTC	1 cut(s)	41
BfaI	CTAG	4 cut(s)	96, 128, 180, 351
BfmI	CTRYAG	1 cut(s)	1242
BisI	GCNGC	4 cut(s)	162, 1186, 1242, 1467
BlsI	GCNGC	4 cut(s)	163, 1187, 1243, 1468
Bme1390I	CCNGG	4 cut(s)	876, 943, 1182, 1233
BmeT110I	CYCGRG	1 cut(s)	1163
BmgBI	CACGTC	1 cut(s)	496
BmgT120I	GGNCC	1 cut(s)	1310
BmiI	GGNNCC	1 cut(s)	300
BmrFI	CCNGG	4 cut(s)	876, 943, 1182, 1233
BmsI	GCATC	9 cut(s)	247, 253, 275, 455, 580, 810, 819, 1127, 1165
BpiI	GAAGAC	2 cut(s)	498, 1143
BplI	GAGNNNNNCTC	2 cut(s)	397, 429
Bpu10I	CCTNAGC	1 cut(s)	549
Bpu14I	TTCGAA	1 cut(s)	607
BsaHI	GRCGYC	1 cut(s)	555
BsaI	GGTCTC	1 cut(s)	41
BsaJI	CCNNGG	6 cut(s)	66, 942, 1162, 1286, 1306, 1384
BsaXI	ACNNNNNCTCC	2 cut(s)	282, 312
Bsc4I	CCNNNNNNNGG	6 cut(s)	763, 812, 925, 1192, 1288, 1451
Bse1I	ACTGG	4 cut(s)	249, 847, 887, 1462
Bse21I	CCTNAGG	1 cut(s)	216
BseBI	CCWGG	4 cut(s)	876, 943, 1182, 1233
BseDI	CCNNGG	6 cut(s)	66, 942, 1162, 1286, 1306, 1384
BseGI	GGATG	8 cut(s)	13, 139, 521, 802, 822, 973, 1071, 1173
BseLI	CCNNNNNNNGG	6 cut(s)	763, 812, 925, 1192, 1288, 1451
BseMII	CTCAG	6 cut(s)	336, 540, 540, 608, 1020, 1139
BseNI	ACTGG	4 cut(s)	249, 847, 887, 1462
BseRI	GAGGAG	2 cut(s)	303, 344
BseXI	GCAGC	2 cut(s)	1228, 1478
BseYI	CCCAGC	1 cut(s)	1495
Bsh1236I	CGCG	1 cut(s)	1157
BshFI	GGCC	3 cut(s)	1185, 1305, 1312
BsiHKCI	CYCGRG	1 cut(s)	1163
BslFI	GGGAC	3 cut(s)	693, 871, 1384
BslI	CCNNNNNNNGG	6 cut(s)	763, 812, 925, 1192, 1288, 1451
BsmAI	GTCTC	1 cut(s)	41
BsmFI	GGGAC	3 cut(s)	693, 871, 1384
BsnI	GGCC	3 cut(s)	1185, 1305, 1312
Bso31I	GGTCTC	1 cut(s)	41
BsoBI	CYCGRG	1 cut(s)	1163
Bsp119I	TTCGAA	1 cut(s)	607
Bsp143I	GATC	3 cut(s)	50, 572, 1262
Bsp19I	CCATGG	1 cut(s)	1286
BspACI	CCGC	3 cut(s)	131, 161, 1186
BspANI	GGCC	3 cut(s)	1185, 1305, 1312
BspCNI	CTCAG	6 cut(s)	337, 539, 541, 607, 1019, 1138
BspFNI	CGCG	1 cut(s)	1157
BspLI	GGNNCC	1 cut(s)	300
BspMAI	CTGCAG	1 cut(s)	1246
BspPI	GGATC	3 cut(s)	58, 580, 1270
BspT104I	TTCGAA	1 cut(s)	607
BspTNI	GGTCTC	1 cut(s)	41
BsrI	ACTGG	4 cut(s)	249, 847, 887, 1462
BssECI	CCNNGG	6 cut(s)	66, 942, 1162, 1286, 1306, 1384
BssMI	GATC	3 cut(s)	50, 572, 1262
BssNI	GRCGYC	1 cut(s)	555
BssT1I	CCWWGG	3 cut(s)	66, 1286, 1306
Bst2UI	CCWGG	4 cut(s)	876, 943, 1182, 1233
Bst4CI	ACNGT	2 cut(s)	284, 838
Bst6I	CTCTTC	4 cut(s)	589, 644, 1104, 1401
BstACI	GRCGYC	1 cut(s)	555
BstAPI	GCANNNNNTGC	1 cut(s)	829
BstBI	TTCGAA	1 cut(s)	607
BstC8I	GCNNGC	4 cut(s)	988, 1381, 1391, 1426
BstDEI	CTNAG	8 cut(s)	26, 216, 345, 526, 549, 594, 1006, 1125
BstDSI	CCRYGG	2 cut(s)	1286, 1384
BstF5I	GGATG	8 cut(s)	13, 139, 521, 802, 822, 973, 1071, 1173
BstFNI	CGCG	1 cut(s)	1157
BstHHI	GCGC	3 cut(s)	363, 1118, 1157
BstKTI	GATC	3 cut(s)	53, 575, 1265
BstMAI	GTCTC	1 cut(s)	41
BstMBI	GATC	3 cut(s)	50, 572, 1262
BstMWI	GCNNNNNNNGC	1 cut(s)	829
BstNI	CCWGG	4 cut(s)	876, 943, 1182, 1233
BstNSI	RCATGY	2 cut(s)	548, 1399
BstSCI	CCNGG	4 cut(s)	874, 941, 1180, 1231
BstSFI	CTRYAG	1 cut(s)	1242
BstUI	CGCG	1 cut(s)	1157
BstV1I	GCAGC	2 cut(s)	1228, 1478
BstV2I	GAAGAC	2 cut(s)	498, 1143
BstX2I	RGATCY	2 cut(s)	572, 1262
BstYI	RGATCY	2 cut(s)	572, 1262
Bsu36I	CCTNAGG	1 cut(s)	216
BsuRI	GGCC	3 cut(s)	1185, 1305, 1312
BtgI	CCRYGG	2 cut(s)	1286, 1384
BtrI	CACGTC	1 cut(s)	496
BtsCI	GGATG	8 cut(s)	13, 139, 521, 802, 822, 973, 1071, 1173
BtsI	GCAGTG	1 cut(s)	716
BtsIMutI	CAGTG	6 cut(s)	213, 256, 534, 716, 1104, 1455
Cac8I	GCNNGC	4 cut(s)	988, 1381, 1391, 1426
CaiI	CAGNNNCTG	1 cut(s)	506
CfoI	GCGC	3 cut(s)	363, 1118, 1157
Cfr13I	GGNCC	1 cut(s)	1310
CseI	GACGC	1 cut(s)	544
DdeI	CTNAG	8 cut(s)	26, 216, 345, 526, 549, 594, 1006, 1125
DpnI	GATC	3 cut(s)	52, 574, 1264
DpnII	GATC	3 cut(s)	50, 572, 1262
EaeI	YGGCCR	1 cut(s)	1183
Eam1104I	CTCTTC	4 cut(s)	589, 644, 1104, 1401
EarI	CTCTTC	4 cut(s)	589, 644, 1104, 1401
Eco130I	CCWWGG	3 cut(s)	66, 1286, 1306
Eco31I	GGTCTC	1 cut(s)	41
Eco57I	CTGAAG	3 cut(s)	528, 1128, 1425
Eco81I	CCTNAGG	1 cut(s)	216
Eco88I	CYCGRG	1 cut(s)	1163
EcoRII	CCWGG	4 cut(s)	874, 941, 1180, 1231
EcoT14I	CCWWGG	3 cut(s)	66, 1286, 1306
EcoT22I	ATGCAT	3 cut(s)	268, 432, 813
ErhI	CCWWGG	3 cut(s)	66, 1286, 1306
FalI	AAGNNNNNCTT	2 cut(s)	209, 241
FaqI	GGGAC	3 cut(s)	693, 871, 1384
FblI	GTMKAC	1 cut(s)	633
Fnu4HI	GCNGC	4 cut(s)	162, 1186, 1242, 1467
FokI	GGATG	8 cut(s)	20, 146, 508, 789, 829, 960, 1078, 1180
Fsp4HI	GCNGC	4 cut(s)	162, 1186, 1242, 1467
FspBI	CTAG	4 cut(s)	96, 128, 180, 351
GlaI	GCGC	3 cut(s)	362, 1117, 1156
GluI	GCNGC	4 cut(s)	162, 1186, 1242, 1467
GsaI	CCCAGC	1 cut(s)	1499
HaeIII	GGCC	3 cut(s)	1185, 1305, 1312
HgaI	GACGC	1 cut(s)	544
HhaI	GCGC	3 cut(s)	363, 1118, 1157
Hin1I	GRCGYC	1 cut(s)	555
Hin6I	GCGC	3 cut(s)	361, 1116, 1155
HinP1I	GCGC	3 cut(s)	361, 1116, 1155
HincII	GTYRAC	1 cut(s)	634
HindII	GTYRAC	1 cut(s)	634
HindIII	AAGCTT	1 cut(s)	683
HinfI	GANTC	9 cut(s)	176, 203, 291, 503, 581, 620, 767, 859, 871
HphI	GGTGA	5 cut(s)	590, 857, 897, 1029, 1097
Hpy166II	GTNNAC	3 cut(s)	634, 730, 848
Hpy188I	TCNGA	6 cut(s)	319, 424, 508, 589, 597, 670
Hpy188III	TCNNGA	5 cut(s)	757, 798, 1165, 1211, 1445
Hpy8I	GTNNAC	3 cut(s)	634, 730, 848
Hpy99I	CGWCG	2 cut(s)	763, 1433
HpyAV	CCTTC	6 cut(s)	101, 592, 603, 721, 757, 1497
HpyCH4III	ACNGT	2 cut(s)	284, 838
HpyCH4IV	ACGT	1 cut(s)	495
HpyF10VI	GCNNNNNNNGC	1 cut(s)	829
HpyF3I	CTNAG	8 cut(s)	26, 216, 345, 526, 549, 594, 1006, 1125
HpySE526I	ACGT	1 cut(s)	495
Hsp92I	GRCGYC	1 cut(s)	555
HspAI	GCGC	3 cut(s)	361, 1116, 1155
Kzo9I	GATC	3 cut(s)	50, 572, 1262
LmnI	GCTCC	1 cut(s)	921
Lsp1109I	GCAGC	2 cut(s)	1228, 1478
LweI	GCATC	9 cut(s)	247, 253, 275, 455, 580, 810, 819, 1127, 1165
MaeI	CTAG	4 cut(s)	96, 128, 180, 351
MaeII	ACGT	1 cut(s)	495
MaeIII	GTNAC	5 cut(s)	460, 578, 838, 1102, 1336
MalI	GATC	3 cut(s)	52, 574, 1264
MboI	GATC	3 cut(s)	50, 572, 1262
MflI	RGATCY	2 cut(s)	572, 1262
MluCI	AATT	8 cut(s)	367, 772, 937, 951, 968, 1033, 1253, 1326
MlyI	GAGTC	3 cut(s)	300, 575, 853
MmeI	TCCRAC	2 cut(s)	550, 906
Mph1103I	ATGCAT	3 cut(s)	268, 432, 813
MroXI	GAANNNNTTC	1 cut(s)	202
MseI	TTAA	2 cut(s)	141, 657
MspR9I	CCNGG	4 cut(s)	876, 943, 1182, 1233
MvaI	CCWGG	4 cut(s)	876, 943, 1182, 1233
MvnI	CGCG	1 cut(s)	1157
MwoI	GCNNNNNNNGC	1 cut(s)	829
NcoI	CCATGG	1 cut(s)	1286
NdeII	GATC	3 cut(s)	50, 572, 1262
NlaIV	GGNNCC	1 cut(s)	300
NmuCI	GTSAC	2 cut(s)	578, 1102
NsiI	ATGCAT	3 cut(s)	268, 432, 813
NspI	RCATGY	2 cut(s)	548, 1399
NspV	TTCGAA	1 cut(s)	607
PciI	ACATGT	1 cut(s)	1395
PdmI	GAANNNNTTC	1 cut(s)	202
PfeI	GAWTC	6 cut(s)	176, 203, 503, 620, 767, 871
PflMI	CCANNNNNTGG	1 cut(s)	812
PfoI	TCCNGGA	1 cut(s)	874
PkrI	GCNGC	4 cut(s)	163, 1187, 1243, 1468
PleI	GAGTC	3 cut(s)	299, 575, 853
PpsI	GAGTC	3 cut(s)	299, 575, 853
PscI	ACATGT	1 cut(s)	1395
Psp6I	CCWGG	4 cut(s)	874, 941, 1180, 1231
PspFI	CCCAGC	1 cut(s)	1495
PspGI	CCWGG	4 cut(s)	874, 941, 1180, 1231
PspN4I	GGNNCC	1 cut(s)	300
PspPI	GGNCC	1 cut(s)	1310
PstI	CTGCAG	1 cut(s)	1246
PstNI	CAGNNNCTG	1 cut(s)	506
PsuI	RGATCY	2 cut(s)	572, 1262
SalI	GTCGAC	1 cut(s)	632
SaqAI	TTAA	2 cut(s)	141, 657
SatI	GCNGC	4 cut(s)	162, 1186, 1242, 1467
Sau3AI	GATC	3 cut(s)	50, 572, 1262
Sau96I	GGNCC	1 cut(s)	1310
SchI	GAGTC	3 cut(s)	300, 575, 853
ScrFI	CCNGG	4 cut(s)	876, 943, 1182, 1233
SfaNI	GCATC	9 cut(s)	247, 253, 275, 455, 580, 810, 819, 1127, 1165
SfcI	CTRYAG	1 cut(s)	1242
SfuI	TTCGAA	1 cut(s)	607
Sse9I	AATT	8 cut(s)	367, 772, 937, 951, 968, 1033, 1253, 1326
SsiI	CCGC	3 cut(s)	131, 161, 1186
SspMI	CTAG	4 cut(s)	96, 128, 180, 351
StyD4I	CCNGG	4 cut(s)	874, 941, 1180, 1231
StyI	CCWWGG	3 cut(s)	66, 1286, 1306
TaaI	ACNGT	2 cut(s)	284, 838
TaiI	ACGT	1 cut(s)	498
TaqI	TCGA	3 cut(s)	607, 633, 827
TasI	AATT	8 cut(s)	367, 772, 937, 951, 968, 1033, 1253, 1326
TauI	GCSGC	2 cut(s)	164, 1188
TfiI	GAWTC	6 cut(s)	176, 203, 503, 620, 767, 871
Tru1I	TTAA	2 cut(s)	141, 657
Tru9I	TTAA	2 cut(s)	141, 657
TscAI	CASTG	6 cut(s)	213, 256, 534, 723, 1111, 1462
TseFI	GTSAC	2 cut(s)	578, 1102
TseI	GCWGC	2 cut(s)	1241, 1466
Tsp45I	GTSAC	2 cut(s)	578, 1102
TspGWI	ACGGA	2 cut(s)	355, 448
TspRI	CASTG	6 cut(s)	213, 256, 534, 723, 1111, 1462
Van91I	CCANNNNNTGG	1 cut(s)	812
XapI	RAATTY	2 cut(s)	367, 1253
XceI	RCATGY	2 cut(s)	548, 1399
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1313
XmiI	GTMKAC	1 cut(s)	633
XmnI	GAANNNNTTC	1 cut(s)	202
XspI	CTAG	4 cut(s)	96, 128, 180, 351
Zsp2I	ATGCAT	3 cut(s)	268, 432, 813

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.