MD15G1217300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011406
Physical Location & Seq
Reverse (-)
17513058 .. 17515146
2089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1217300.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGATTGGGGTTCGAATGGAGAAGAGCCCACTTCGTGGGAAGAAAATTACAGTGTAAATTTGATTCCATCGGAGTTATTTTTCAAGTTCCGGAAAGAAGTGCAGGGTTTAAGGGTCGGCCTCAATCTGGAGTTCTATAATGCTCCAATCAATGAGTTTCAAGGAAAGATTGTTTTGAAGCCGTTAGCTCCTGAACGGACTTGGAAGTTCATCTACGAGCCTATCCATCAGGATGTTCGTGTTCTTTCGAAAAAGATTCCTCTAACTGGATTTCTAAATCTCCAGGTTGGCATCGGCCATAATTTTCAAACGAATGCAATTGGATGGAAATGGAAACTTACTACTTGTTTTGGTGGAGATGGTATATCTCGTACTAGGAGTAAGACATCGGTTCGCTTGGTTCCTGGTGTGGATTTGCGCTTTGGGTGGAGGGCTGATTATGTACTTCCAGAAATCACAGGGGCTCTGGGTACTGGTGAACCATGGTTCAACATGAACTCGGGAAAGCTGCAAGCATCGCTAGATAGAGTTGAGACCATTGTAACCTATACTGATATCTACAGTTTATACAAGCCTAAAGGCCAGGATCAGCAGCAGAGAACTGAAACGGATGTAATTTCTTATTCAAGTCCTTCAGATCCCTTGAAATCAAGCGACTTTTCGTCACACAATGACCGTTCTACATGGATCAATGGACCAGAGGATGCAGCAGATTTACACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

242

Amino Acids

27.38

Weight (kDa)

6.12

Isoelectric Point (pI)

30.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7781 PF25003 12 - 182 1.9e-87 Domain of unknown function (DUF7781)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011406)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G06010
fragaria_vesca FvH4_1g08630 FvH4_1g08630
malus_domestica MD02G1091100.v1.1 MD15G1217300.v1.1
prunus_persica Prupe.7G199200_v2.0.a1 Prupe.7G199200_v2.0.a1 Prupe.7G199200_v2.0.a1
pyrus_communis pycom02g07260 pycom15g19290
rosa_chinensis RchiOBHm_Chr2g0094901
rosa_laevigata RLG00000016529
rosa_multiflora Rmu_sc0033193.1_g000001
rosa_roxburghii Rroxscaffold_2G00146790
rosa_rugosa Rorug02G0048800
rosa_samantha Rh2AG095300 Rh2BG096300 Rh2CG098300 Rh2DG095000
rosa_wichuraiana Rw2G007170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 36
AccIII TCCGGA 1 cut(s) 90
AclWI GGATC 3 cut(s) 594, 632, 695
AcoI YGGCCR 1 cut(s) 295
AcsI RAATTY 1 cut(s) 58
AcuI CTGAAG 1 cut(s) 618
AdeI CACNNNGTG 1 cut(s) 36
AfaI GTAC 3 cut(s) 373, 444, 472
AfiI CCNNNNNNNGG 3 cut(s) 36, 127, 266
AgsI TTSAA 7 cut(s) 85, 161, 178, 308, 490, 627, 646
AhdI GACNNNNNGTC 1 cut(s) 661
AjnI CCWGG 3 cut(s) 282, 403, 582
AluBI AGCT 2 cut(s) 188, 508
AluI AGCT 2 cut(s) 188, 508
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 3 cut(s) 594, 632, 695
Ama87I CYCGRG 1 cut(s) 499
Aor13HI TCCGGA 1 cut(s) 90
AoxI GGCC 3 cut(s) 118, 295, 580
ApeKI GCWGC 3 cut(s) 508, 592, 707
ApoI RAATTY 1 cut(s) 58
AspLEI GCGC 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 695
AsuHPI GGTGA 1 cut(s) 488
AsuII TTCGAA 2 cut(s) 14, 248
AvaI CYCGRG 1 cut(s) 499
AvaII GGWCC 1 cut(s) 695
BanII GRGCYC 2 cut(s) 30, 466
BarI GAAGNNNNNNTAC 2 cut(s) 197, 229
BbvI GCAGC 3 cut(s) 495, 604, 719
BccI CCATC 4 cut(s) 76, 234, 318, 353
BceAI ACGGC 1 cut(s) 166
BciT130I CCWGG 3 cut(s) 284, 405, 584
BcoDI GTCTC 1 cut(s) 527
BfaI CTAG 2 cut(s) 375, 521
BfmI CTRYAG 1 cut(s) 559
BisI GCNGC 3 cut(s) 509, 593, 708
BlsI GCNGC 3 cut(s) 510, 594, 709
Bme1390I CCNGG 3 cut(s) 284, 405, 584
Bme18I GGWCC 1 cut(s) 695
BmeRI GACNNNNNGTC 1 cut(s) 661
BmeT110I CYCGRG 1 cut(s) 499
BmgT120I GGNCC 1 cut(s) 695
BmiI GGNNCC 1 cut(s) 402
BmrFI CCNGG 3 cut(s) 284, 405, 584
BmsI GCATC 3 cut(s) 300, 524, 694
BpmI CTGGAG 2 cut(s) 149, 266
Bpu14I TTCGAA 2 cut(s) 14, 248
BsaI GGTCTC 1 cut(s) 527
BsaJI CCNNGG 1 cut(s) 482
BsaWI WCCGGW 1 cut(s) 90
Bsc4I CCNNNNNNNGG 3 cut(s) 36, 127, 266
Bse1I ACTGG 2 cut(s) 271, 478
BseAI TCCGGA 1 cut(s) 90
BseBI CCWGG 3 cut(s) 284, 405, 584
BseDI CCNNGG 1 cut(s) 482
BseGI GGATG 4 cut(s) 238, 329, 616, 709
BseLI CCNNNNNNNGG 3 cut(s) 36, 127, 266
BseNI ACTGG 2 cut(s) 271, 478
BseXI GCAGC 3 cut(s) 495, 604, 719
BsgI GTGCAG 1 cut(s) 122
BshFI GGCC 3 cut(s) 120, 297, 582
BsiHKCI CYCGRG 1 cut(s) 499
BsiSI CCGG 1 cut(s) 91
BslI CCNNNNNNNGG 3 cut(s) 36, 127, 266
BsmAI GTCTC 1 cut(s) 527
BsmI GAATGC 1 cut(s) 319
BsnI GGCC 3 cut(s) 120, 297, 582
Bso31I GGTCTC 1 cut(s) 527
BsoBI CYCGRG 1 cut(s) 499
Bsp119I TTCGAA 2 cut(s) 14, 248
Bsp1286I GDGCHC 2 cut(s) 30, 466
Bsp13I TCCGGA 1 cut(s) 90
Bsp143I GATC 3 cut(s) 586, 637, 687
Bsp19I CCATGG 1 cut(s) 482
BspANI GGCC 3 cut(s) 120, 297, 582
BspEI TCCGGA 1 cut(s) 90
BspLI GGNNCC 1 cut(s) 402
BspPI GGATC 3 cut(s) 594, 632, 695
BspQI GCTCTTC 1 cut(s) 18
BspT104I TTCGAA 2 cut(s) 14, 248
BspTNI GGTCTC 1 cut(s) 527
BsrI ACTGG 2 cut(s) 271, 478
BssECI CCNNGG 1 cut(s) 482
BssMI GATC 3 cut(s) 586, 637, 687
BssT1I CCWWGG 1 cut(s) 482
Bst2UI CCWGG 3 cut(s) 284, 405, 584
Bst4CI ACNGT 3 cut(s) 53, 563, 677
Bst6I CTCTTC 1 cut(s) 18
BstBI TTCGAA 2 cut(s) 14, 248
BstC8I GCNNGC 1 cut(s) 513
BstDSI CCRYGG 1 cut(s) 482
BstF5I GGATG 4 cut(s) 238, 329, 616, 709
BstHHI GCGC 1 cut(s) 420
BstKTI GATC 3 cut(s) 589, 640, 690
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 3 cut(s) 586, 637, 687
BstMWI GCNNNNNNNGC 1 cut(s) 517
BstNI CCWGG 3 cut(s) 284, 405, 584
BstSCI CCNGG 3 cut(s) 282, 403, 582
BstSFI CTRYAG 1 cut(s) 559
BstV1I GCAGC 3 cut(s) 495, 604, 719
BstX2I RGATCY 1 cut(s) 637
BstYI RGATCY 1 cut(s) 637
BsuRI GGCC 3 cut(s) 120, 297, 582
BtgI CCRYGG 1 cut(s) 482
BtgZI GCGATG 1 cut(s) 501
BtsCI GGATG 4 cut(s) 238, 329, 616, 709
BtsIMutI CAGTG 1 cut(s) 58
Cac8I GCNNGC 1 cut(s) 513
CfoI GCGC 1 cut(s) 420
Cfr13I GGNCC 1 cut(s) 695
Csp6I GTAC 3 cut(s) 372, 443, 471
CviAII CATG 3 cut(s) 483, 493, 684
CviQI GTAC 3 cut(s) 372, 443, 471
DpnI GATC 3 cut(s) 588, 639, 689
DpnII GATC 3 cut(s) 586, 637, 687
DraIII CACNNNGTG 1 cut(s) 36
DriI GACNNNNNGTC 1 cut(s) 661
EaeI YGGCCR 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 18
Eam1105I GACNNNNNGTC 1 cut(s) 661
EarI CTCTTC 1 cut(s) 18
Eco130I CCWWGG 1 cut(s) 482
Eco24I GRGCYC 2 cut(s) 30, 466
Eco31I GGTCTC 1 cut(s) 527
Eco32I GATATC 1 cut(s) 556
Eco47I GGWCC 1 cut(s) 695
Eco57I CTGAAG 1 cut(s) 618
Eco88I CYCGRG 1 cut(s) 499
EcoRII CCWGG 3 cut(s) 282, 403, 582
EcoRV GATATC 1 cut(s) 556
EcoT14I CCWWGG 1 cut(s) 482
EcoT38I GRGCYC 2 cut(s) 30, 466
ErhI CCWWGG 1 cut(s) 482
FaeI CATG 3 cut(s) 486, 496, 687
FaiI YATR 9 cut(s) 138, 300, 365, 441, 484, 494, 549, 568, 685
FatI CATG 3 cut(s) 482, 492, 683
Fnu4HI GCNGC 3 cut(s) 509, 593, 708
FokI GGATG 4 cut(s) 245, 336, 623, 716
FriOI GRGCYC 2 cut(s) 30, 466
Fsp4HI GCNGC 3 cut(s) 509, 593, 708
FspBI CTAG 2 cut(s) 375, 521
GlaI GCGC 1 cut(s) 419
GluI GCNGC 3 cut(s) 509, 593, 708
GsuI CTGGAG 2 cut(s) 149, 266
HaeIII GGCC 3 cut(s) 120, 297, 582
HapII CCGG 1 cut(s) 91
HhaI GCGC 1 cut(s) 420
Hin1II CATG 3 cut(s) 486, 496, 687
Hin6I GCGC 1 cut(s) 418
HinP1I GCGC 1 cut(s) 418
HinfI GANTC 2 cut(s) 64, 256
HpaII CCGG 1 cut(s) 91
HphI GGTGA 1 cut(s) 488
Hpy166II GTNNAC 1 cut(s) 479
Hpy188I TCNGA 2 cut(s) 73, 637
Hpy188III TCNNGA 6 cut(s) 91, 128, 191, 230, 449, 501
Hpy8I GTNNAC 1 cut(s) 479
HpyAV CCTTC 1 cut(s) 642
HpyCH4III ACNGT 3 cut(s) 53, 563, 677
HpyCH4V TGCA 4 cut(s) 103, 317, 511, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 517
Hsp92II CATG 3 cut(s) 486, 496, 687
HspAI GCGC 1 cut(s) 418
Kpn2I TCCGGA 1 cut(s) 90
Kzo9I GATC 3 cut(s) 586, 637, 687
LguI GCTCTTC 1 cut(s) 18
LmnI GCTCC 2 cut(s) 148, 193
Lsp1109I GCAGC 3 cut(s) 495, 604, 719
LweI GCATC 3 cut(s) 300, 524, 694
MaeI CTAG 2 cut(s) 375, 521
MaeIII GTNAC 2 cut(s) 541, 663
MalI GATC 3 cut(s) 588, 639, 689
MboI GATC 3 cut(s) 586, 637, 687
MboII GAAGA 2 cut(s) 35, 53
MfeI CAATTG 1 cut(s) 318
MflI RGATCY 1 cut(s) 637
MhlI GDGCHC 2 cut(s) 30, 466
MluCI AATT 5 cut(s) 46, 58, 301, 318, 615
MnlI CCTC 4 cut(s) 131, 270, 423, 694
MroI TCCGGA 1 cut(s) 90
MseI TTAA 1 cut(s) 110
MslI CAYNNNNRTG 1 cut(s) 231
MspI CCGG 1 cut(s) 91
MspR9I CCNGG 3 cut(s) 284, 405, 584
MunI CAATTG 1 cut(s) 318
Mva1269I GAATGC 1 cut(s) 319
MvaI CCWGG 3 cut(s) 284, 405, 584
MwoI GCNNNNNNNGC 1 cut(s) 517
NcoI CCATGG 1 cut(s) 482
NdeII GATC 3 cut(s) 586, 637, 687
NlaIII CATG 3 cut(s) 486, 496, 687
NlaIV GGNNCC 1 cut(s) 402
NmuCI GTSAC 1 cut(s) 663
NspV TTCGAA 2 cut(s) 14, 248
PciSI GCTCTTC 1 cut(s) 18
PctI GAATGC 1 cut(s) 319
PfeI GAWTC 2 cut(s) 64, 256
PflMI CCANNNNNTGG 1 cut(s) 36
PkrI GCNGC 3 cut(s) 510, 594, 709
Psp6I CCWGG 3 cut(s) 282, 403, 582
PspGI CCWGG 3 cut(s) 282, 403, 582
PspN4I GGNNCC 1 cut(s) 402
PspPI GGNCC 1 cut(s) 695
PsuI RGATCY 1 cut(s) 637
RsaI GTAC 3 cut(s) 373, 444, 472
RsaNI GTAC 3 cut(s) 372, 443, 471
RseI CAYNNNNRTG 1 cut(s) 231
SapI GCTCTTC 1 cut(s) 18
SaqAI TTAA 1 cut(s) 110
SatI GCNGC 3 cut(s) 509, 593, 708
Sau3AI GATC 3 cut(s) 586, 637, 687
Sau96I GGNCC 1 cut(s) 695
ScrFI CCNGG 3 cut(s) 284, 405, 584
SduI GDGCHC 2 cut(s) 30, 466
SetI ASST 4 cut(s) 190, 288, 510, 548
SfaNI GCATC 3 cut(s) 300, 524, 694
SfcI CTRYAG 1 cut(s) 559
SfuI TTCGAA 2 cut(s) 14, 248
SinI GGWCC 1 cut(s) 695
SmiMI CAYNNNNRTG 1 cut(s) 231
Sse9I AATT 5 cut(s) 46, 58, 301, 318, 615
SspMI CTAG 2 cut(s) 375, 521
StyD4I CCNGG 3 cut(s) 282, 403, 582
StyI CCWWGG 1 cut(s) 482
TaaI ACNGT 3 cut(s) 53, 563, 677
TaqI TCGA 2 cut(s) 14, 248
TasI AATT 5 cut(s) 46, 58, 301, 318, 615
TatI WGTACW 1 cut(s) 442
TfiI GAWTC 2 cut(s) 64, 256
Tru1I TTAA 1 cut(s) 110
Tru9I TTAA 1 cut(s) 110
TscAI CASTG 1 cut(s) 58
TseFI GTSAC 1 cut(s) 663
TseI GCWGC 3 cut(s) 508, 592, 707
Tsp45I GTSAC 1 cut(s) 663
TspDTI ATGAA 2 cut(s) 199, 509
TspGWI ACGGA 2 cut(s) 211, 623
TspRI CASTG 1 cut(s) 58
Van91I CCANNNNNTGG 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 695
XapI RAATTY 1 cut(s) 58
XspI CTAG 2 cut(s) 375, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.