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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 249, 566 |
| AccII | CGCG | 1 cut(s) | 309 |
| AciI | CCGC | 3 cut(s) | 173, 307, 417 |
| AclWI | GGATC | 1 cut(s) | 236 |
| AcuI | CTGAAG | 2 cut(s) | 259, 396 |
| AfaI | GTAC | 1 cut(s) | 5 |
| AgsI | TTSAA | 2 cut(s) | 163, 223 |
| AluBI | AGCT | 2 cut(s) | 167, 270 |
| AluI | AGCT | 2 cut(s) | 167, 270 |
| Alw21I | GWGCWC | 1 cut(s) | 530 |
| AlwI | GGATC | 1 cut(s) | 236 |
| ApeKI | GCWGC | 2 cut(s) | 496, 541 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 236 |
| AspS9I | GGNCC | 3 cut(s) | 37, 92, 419 |
| AsuHPI | GGTGA | 2 cut(s) | 140, 296 |
| AvaII | GGWCC | 3 cut(s) | 37, 92, 419 |
| BanII | GRGCYC | 1 cut(s) | 141 |
| Bbv12I | GWGCWC | 1 cut(s) | 530 |
| BbvI | GCAGC | 2 cut(s) | 508, 528 |
| BccI | CCATC | 1 cut(s) | 467 |
| BceAI | ACGGC | 1 cut(s) | 387 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 89, 123 |
| BfaI | CTAG | 1 cut(s) | 431 |
| BglII | AGATCT | 2 cut(s) | 50, 433 |
| BisI | GCNGC | 2 cut(s) | 497, 542 |
| BlsI | GCNGC | 2 cut(s) | 498, 543 |
| Bme18I | GGWCC | 3 cut(s) | 37, 92, 419 |
| BmgT120I | GGNCC | 3 cut(s) | 37, 92, 419 |
| BmiI | GGNNCC | 2 cut(s) | 93, 138 |
| BmsI | GCATC | 2 cut(s) | 370, 483 |
| BsaAI | YACGTR | 2 cut(s) | 461, 554 |
| Bse1I | ACTGG | 2 cut(s) | 245, 343 |
| BseGI | GGATG | 1 cut(s) | 262 |
| BseMII | CTCAG | 1 cut(s) | 38 |
| BseNI | ACTGG | 2 cut(s) | 245, 343 |
| BseXI | GCAGC | 2 cut(s) | 508, 528 |
| Bsh1236I | CGCG | 1 cut(s) | 309 |
| BsiHKAI | GWGCWC | 1 cut(s) | 530 |
| BslFI | GGGAC | 1 cut(s) | 105 |
| BsmFI | GGGAC | 1 cut(s) | 105 |
| Bsp1286I | GDGCHC | 2 cut(s) | 141, 530 |
| Bsp1407I | TGTACA | 1 cut(s) | 3 |
| Bsp143I | GATC | 3 cut(s) | 50, 241, 433 |
| BspACI | CCGC | 3 cut(s) | 173, 307, 417 |
| BspCNI | CTCAG | 1 cut(s) | 39 |
| BspFNI | CGCG | 1 cut(s) | 309 |
| BspHI | TCATGA | 1 cut(s) | 394 |
| BspLI | GGNNCC | 2 cut(s) | 93, 138 |
| BspPI | GGATC | 1 cut(s) | 236 |
| BsrGI | TGTACA | 1 cut(s) | 3 |
| BsrI | ACTGG | 2 cut(s) | 245, 343 |
| BssMI | GATC | 3 cut(s) | 50, 241, 433 |
| BssNAI | GTATAC | 1 cut(s) | 250 |
| Bst1107I | GTATAC | 1 cut(s) | 250 |
| Bst4CI | ACNGT | 2 cut(s) | 152, 227 |
| BstAUI | TGTACA | 1 cut(s) | 3 |
| BstBAI | YACGTR | 2 cut(s) | 461, 554 |
| BstC8I | GCNNGC | 1 cut(s) | 119 |
| BstDEI | CTNAG | 1 cut(s) | 47 |
| BstF5I | GGATG | 1 cut(s) | 262 |
| BstFNI | CGCG | 1 cut(s) | 309 |
| BstKTI | GATC | 3 cut(s) | 53, 244, 436 |
| BstMBI | GATC | 3 cut(s) | 50, 241, 433 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 380 |
| BstNSI | RCATGY | 1 cut(s) | 104 |
| BstUI | CGCG | 1 cut(s) | 309 |
| BstV1I | GCAGC | 2 cut(s) | 508, 528 |
| BstX2I | RGATCY | 2 cut(s) | 50, 433 |
| BstYI | RGATCY | 2 cut(s) | 50, 433 |
| BstZ17I | GTATAC | 1 cut(s) | 250 |
| BtsCI | GGATG | 1 cut(s) | 262 |
| BtsIMutI | CAGTG | 3 cut(s) | 67, 232, 336 |
| Cac8I | GCNNGC | 1 cut(s) | 119 |
| CciI | TCATGA | 1 cut(s) | 394 |
| Cfr13I | GGNCC | 3 cut(s) | 37, 92, 419 |
| Csp6I | GTAC | 1 cut(s) | 4 |
| CviAII | CATG | 4 cut(s) | 101, 122, 395, 425 |
| CviJI | RGCY | 8 cut(s) | 23, 29, 139, 167, 270, 314, 374, 499 |
| CviKI_1 | RGCY | 8 cut(s) | 23, 29, 139, 167, 270, 314, 374, 499 |
| CviQI | GTAC | 1 cut(s) | 4 |
| DdeI | CTNAG | 1 cut(s) | 47 |
| DpnI | GATC | 3 cut(s) | 52, 243, 435 |
| DpnII | GATC | 3 cut(s) | 50, 241, 433 |
| Eco24I | GRGCYC | 1 cut(s) | 141 |
| Eco47I | GGWCC | 3 cut(s) | 37, 92, 419 |
| Eco57I | CTGAAG | 2 cut(s) | 259, 396 |
| EcoO109I | RGGNCCY | 1 cut(s) | 92 |
| EcoT38I | GRGCYC | 1 cut(s) | 141 |
| FaeI | CATG | 4 cut(s) | 104, 125, 398, 428 |
| FaqI | GGGAC | 1 cut(s) | 105 |
| FatI | CATG | 4 cut(s) | 100, 121, 394, 424 |
| FauNDI | CATATG | 2 cut(s) | 143, 283 |
| FblI | GTMKAC | 2 cut(s) | 249, 566 |
| Fnu4HI | GCNGC | 2 cut(s) | 497, 542 |
| FokI | GGATG | 1 cut(s) | 269 |
| FriOI | GRGCYC | 1 cut(s) | 141 |
| Fsp4HI | GCNGC | 2 cut(s) | 497, 542 |
| FspBI | CTAG | 1 cut(s) | 431 |
| GluI | GCNGC | 2 cut(s) | 497, 542 |
| Hin1II | CATG | 4 cut(s) | 104, 125, 398, 428 |
| HincII | GTYRAC | 1 cut(s) | 567 |
| HindII | GTYRAC | 1 cut(s) | 567 |
| HindIII | AAGCTT | 2 cut(s) | 165, 268 |
| HinfI | GANTC | 3 cut(s) | 205, 520, 557 |
| HphI | GGTGA | 2 cut(s) | 140, 296 |
| Hpy166II | GTNNAC | 2 cut(s) | 250, 567 |
| Hpy188I | TCNGA | 2 cut(s) | 48, 256 |
| Hpy188III | TCNNGA | 3 cut(s) | 160, 395, 431 |
| Hpy8I | GTNNAC | 2 cut(s) | 250, 567 |
| HpyAV | CCTTC | 1 cut(s) | 403 |
| HpyCH4III | ACNGT | 2 cut(s) | 152, 227 |
| HpyCH4IV | ACGT | 3 cut(s) | 402, 460, 553 |
| HpyCH4V | TGCA | 5 cut(s) | 100, 121, 366, 496, 544 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 380 |
| HpyF3I | CTNAG | 1 cut(s) | 47 |
| HpySE526I | ACGT | 3 cut(s) | 402, 460, 553 |
| Hsp92II | CATG | 4 cut(s) | 104, 125, 398, 428 |
| Kzo9I | GATC | 3 cut(s) | 50, 241, 433 |
| LmnI | GCTCC | 2 cut(s) | 136, 533 |
| LpnPI | CCDG | 7 cut(s) | 131, 258, 262, 324, 340, 462, 483 |
| Lsp1109I | GCAGC | 2 cut(s) | 508, 528 |
| LweI | GCATC | 2 cut(s) | 370, 483 |
| MaeI | CTAG | 1 cut(s) | 431 |
| MaeII | ACGT | 3 cut(s) | 402, 460, 553 |
| MaeIII | GTNAC | 1 cut(s) | 146 |
| MalI | GATC | 3 cut(s) | 52, 243, 435 |
| MboI | GATC | 3 cut(s) | 50, 241, 433 |
| MboII | GAAGA | 6 cut(s) | 209, 211, 244, 265, 389, 475 |
| MflI | RGATCY | 2 cut(s) | 50, 433 |
| MhlI | GDGCHC | 2 cut(s) | 141, 530 |
| MlyI | GAGTC | 1 cut(s) | 199 |
| MnlI | CCTC | 2 cut(s) | 40, 432 |
| MroXI | GAANNNNTTC | 1 cut(s) | 236 |
| MseI | TTAA | 3 cut(s) | 83, 264, 405 |
| MslI | CAYNNNNRTG | 1 cut(s) | 153 |
| MvnI | CGCG | 1 cut(s) | 309 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 380 |
| NdeI | CATATG | 2 cut(s) | 143, 283 |
| NdeII | GATC | 3 cut(s) | 50, 241, 433 |
| NlaIII | CATG | 4 cut(s) | 104, 125, 398, 428 |
| NlaIV | GGNNCC | 2 cut(s) | 93, 138 |
| NmuCI | GTSAC | 1 cut(s) | 146 |
| NspI | RCATGY | 1 cut(s) | 104 |
| PagI | TCATGA | 1 cut(s) | 394 |
| PdmI | GAANNNNTTC | 1 cut(s) | 236 |
| PfeI | GAWTC | 2 cut(s) | 520, 557 |
| PkrI | GCNGC | 2 cut(s) | 498, 543 |
| PleI | GAGTC | 1 cut(s) | 199 |
| PpsI | GAGTC | 1 cut(s) | 199 |
| Ppu21I | YACGTR | 2 cut(s) | 461, 554 |
| PpuMI | RGGWCCY | 1 cut(s) | 92 |
| Psp5II | RGGWCCY | 1 cut(s) | 92 |
| PspN4I | GGNNCC | 2 cut(s) | 93, 138 |
| PspPI | GGNCC | 3 cut(s) | 37, 92, 419 |
| PspPPI | RGGWCCY | 1 cut(s) | 92 |
| PsuI | RGATCY | 2 cut(s) | 50, 433 |
| RsaI | GTAC | 1 cut(s) | 5 |
| RsaNI | GTAC | 1 cut(s) | 4 |
| RseI | CAYNNNNRTG | 1 cut(s) | 153 |
| SalI | GTCGAC | 1 cut(s) | 565 |
| SaqAI | TTAA | 3 cut(s) | 83, 264, 405 |
| SatI | GCNGC | 2 cut(s) | 497, 542 |
| Sau3AI | GATC | 3 cut(s) | 50, 241, 433 |
| Sau96I | GGNCC | 3 cut(s) | 37, 92, 419 |
| SchI | GAGTC | 1 cut(s) | 199 |
| SduI | GDGCHC | 2 cut(s) | 141, 530 |
| SfaNI | GCATC | 2 cut(s) | 370, 483 |
| SinI | GGWCC | 3 cut(s) | 37, 92, 419 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 153 |
| SsiI | CCGC | 3 cut(s) | 173, 307, 417 |
| SspMI | CTAG | 1 cut(s) | 431 |
| TaaI | ACNGT | 2 cut(s) | 152, 227 |
| TaiI | ACGT | 3 cut(s) | 405, 463, 556 |
| TaqI | TCGA | 3 cut(s) | 109, 240, 566 |
| TatI | WGTACW | 1 cut(s) | 3 |
| TfiI | GAWTC | 2 cut(s) | 520, 557 |
| Tru1I | TTAA | 3 cut(s) | 83, 264, 405 |
| Tru9I | TTAA | 3 cut(s) | 83, 264, 405 |
| TscAI | CASTG | 3 cut(s) | 67, 232, 343 |
| TseFI | GTSAC | 1 cut(s) | 146 |
| TseI | GCWGC | 2 cut(s) | 496, 541 |
| Tsp45I | GTSAC | 1 cut(s) | 146 |
| TspDTI | ATGAA | 2 cut(s) | 210, 383 |
| TspRI | CASTG | 3 cut(s) | 67, 232, 343 |
| VpaK11BI | GGWCC | 3 cut(s) | 37, 92, 419 |
| XbaI | TCTAGA | 1 cut(s) | 430 |
| XceI | RCATGY | 1 cut(s) | 104 |
| XmiI | GTMKAC | 2 cut(s) | 249, 566 |
| XmnI | GAANNNNTTC | 1 cut(s) | 236 |
| XspI | CTAG | 1 cut(s) | 431 |