MD15G1217400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000612
Physical Location & Seq
Reverse (-)
17519783 .. 17520685
903 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1217400.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGCTTCGAGGTCTGCTCTTTTCACAGAGGAGAAGTTCAAACAGTTTCACAACATGGACCGAAATCTGTACTCAATTCTGGTGCTAAAGCTCTTCCGGGACCCACTGGAGTCCATGCAGGTACTGGCCCTGTGGCTCTGGCTGGAGAGAGTTGGGTTCAAGAACGTGGTGAAAAAGATGCTCTCTCTGCCATACATTTTGATCAACGAGCTCGCTGACGAGTCCATCACTTGTCTCGAACTCATCAGAGGGACCCATTTCTCTCTTCCGTCTGAGCAGACTGACATCCCACTCATACAGAGTTTTATAGAGAAGGAAATCTCAGTCCAGTTTTTTCACCAGAACCGGGACGCAGCGGCTCGAGACGTCACTAACGCCGTCAACGAGGTCTGCCTCCCGGCTTTCGACGACATTATGCAGAAGGCAATGCAACGGGATTCTGCTCAGAACTTTGCGGATCACAGTTACGTAGTGTTACCTTCACAACGATCGTCTTCGTCTTTATTCCCATTTTCTCTTTCAATTAATCAGCAGCCGTGTCTGCAGCAGCAACCTCTGCAGAGGAACGAGGCGACGCCACCACACGACAGGACGATGTTTGTGACGTTCTCAAAAGGGTACCCGGTGCAGGAGTCCGAGGTGAGGCAGTTTTTCAAAATAACTTTCGGGGCTCGAATCGAGTCGGTGCAGATGCAGGAGGTGCAGCCATACGAGCAATCACTGTTTGCGATAATTGTCTTCCACTCTTCGGCGACGATCGAGGCCATACTCAACGGGATGGGCAAGGCCAAGTTCACAATCAATGGAAAACACGTTTGGGTGAGGAAATATATGCCGAGAAGGACACGATCTTTGCTTCCACCAATGCTTTGGCAACCAGCAATTGCTCCTTTTGGGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

34.52

Weight (kDa)

8.39

Isoelectric Point (pI)

57.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000612)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G49290 AT5G13620
fragaria_vesca FvH4_1g08631 FvH4_2g11491 FvH4_3g26240 FvH4_7g20310 FvH4_7g20310 FvH4_7g20311
malus_domestica MD02G1091200.v1.1 MD07G1180400.v1.1 MD15G1217400.v1.1
prunus_persica Prupe.2G219300_v2.0.a1 Prupe.2G219700_v2.0.a1 Prupe.7G199100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0364241 RchiOBHm_Chr1g0364261 RchiOBHm_Chr2g0094911 RchiOBHm_Chr5g0048831 RchiOBHm_Chr5g0048891 RchiOBHm_Chr5g0048921 RchiOBHm_Chr6g0270221
rosa_laevigata RLG00000013804 RLG00000016530 RLG00000027499 RLG00000027502 RLG00000027503 RLG00000034558
rosa_multiflora Rmu_co8509593.1_g000001 Rmu_sc0002138.1_g000006 Rmu_sc0003628.1_g000024 Rmu_sc0006611.1_g000002 Rmu_sc0011253.1_g000002 Rmu_sc0011664.1_g000001 Rmu_sc0023830.1_g000002 Rmu_sc0023830.1_g000003
rosa_roxburghii Rroxscaffold_1G00033000 Rroxscaffold_2G00146780 Rroxscaffold_4G00292480 Rroxscaffold_4G00292520 Rroxscaffold_4G00292540 Rroxscaffold_7G00198040
rosa_rugosa Rorug01G0315200 Rorug01G0315300.1 Rorug02G0048800 Rorug05G0241300 Rorug06G0053800
rosa_samantha Rh1AG322400 Rh1AG322500 Rh1BG285300 Rh1BG285400 Rh1CG301700 Rh1CG301900 Rh1DG316700 Rh1DG316800 Rh2AG095400 Rh2BG096400 Rh2CG098400 Rh2DG095100 Rh5AG321000 Rh5AG321300 Rh5BG331300 Rh5BG331400 Rh5BG331500 Rh5CG356900 Rh5DG342800 Rh5DG342900 Rh5DG343100 Rh5DG343300 Rh6AG172300 Rh6BG176600 Rh6CG172500 Rh6DG163800
rosa_wichuraiana Rw1G028560 Rw1G028570 Rw2G007180 Rw5G030080 Rw5G030110 Rw5G030290 Rw5G030320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 369
Acc36I ACCTGC 1 cut(s) 109
Acc65I GGTACC 1 cut(s) 618
AccB1I GGYRCC 1 cut(s) 618
AciI CCGC 2 cut(s) 356, 455
AclWI GGATC 1 cut(s) 465
AcyI GRCGYC 2 cut(s) 366, 575
AfaI GTAC 3 cut(s) 71, 123, 620
AfiI CCNNNNNNNGG 5 cut(s) 346, 628, 642, 748, 896
AflIII ACRYGT 1 cut(s) 811
AgsI TTSAA 4 cut(s) 40, 160, 522, 655
AjuI GAANNNNNNNTTGG 2 cut(s) 799, 831
AluBI AGCT 2 cut(s) 91, 211
AluI AGCT 2 cut(s) 91, 211
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 2 cut(s) 239, 357
AlwI GGATC 1 cut(s) 465
AlwNI CAGNNNCTG 1 cut(s) 124
Ama87I CYCGRG 1 cut(s) 360
AoxI GGCC 3 cut(s) 126, 762, 786
ApeKI GCWGC 5 cut(s) 353, 532, 544, 547, 703
ArsI GACNNNNNNTTYG 2 cut(s) 835, 867
AseI ATTAAT 1 cut(s) 525
Asp718I GGTACC 1 cut(s) 618
AspS9I GGNCC 4 cut(s) 58, 100, 127, 252
AsuC2I CCSGG 4 cut(s) 98, 347, 398, 623
AsuHPI GGTGA 4 cut(s) 181, 329, 652, 832
AvaI CYCGRG 1 cut(s) 360
AvaII GGWCC 3 cut(s) 58, 100, 252
BanI GGYRCC 1 cut(s) 618
BanII GRGCYC 2 cut(s) 213, 673
BbsI GAAGAC 2 cut(s) 486, 730
Bbv12I GWGCWC 1 cut(s) 213
BbvI GCAGC 5 cut(s) 365, 544, 556, 559, 715
BccI CCATC 2 cut(s) 233, 772
BceAI ACGGC 2 cut(s) 362, 520
BclI TGATCA 1 cut(s) 201
BcnI CCSGG 4 cut(s) 98, 347, 398, 623
BcoDI GTCTC 2 cut(s) 239, 357
BfmI CTRYAG 2 cut(s) 542, 557
BfuAI ACCTGC 1 cut(s) 109
BisI GCNGC 6 cut(s) 354, 357, 533, 545, 548, 704
BlsI GCNGC 6 cut(s) 355, 358, 534, 546, 549, 705
Bme1390I CCNGG 4 cut(s) 98, 347, 398, 623
Bme18I GGWCC 3 cut(s) 58, 100, 252
BmeT110I CYCGRG 1 cut(s) 360
BmgT120I GGNCC 4 cut(s) 58, 100, 127, 252
BmiI GGNNCC 5 cut(s) 101, 102, 253, 254, 620
BmrFI CCNGG 4 cut(s) 98, 347, 398, 623
BmsI GCATC 2 cut(s) 168, 681
BpiI GAAGAC 2 cut(s) 486, 730
BplI GAGNNNNNCTC 1 cut(s) 33
BpmI CTGGAG 2 cut(s) 128, 164
BpuMI CCSGG 4 cut(s) 98, 347, 398, 623
BsaAI YACGTR 1 cut(s) 469
BsaHI GRCGYC 2 cut(s) 366, 575
BsaJI CCNNGG 1 cut(s) 636
Bsc4I CCNNNNNNNGG 5 cut(s) 346, 628, 642, 748, 896
Bse1I ACTGG 3 cut(s) 111, 129, 328
Bse3DI GCAATG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 636
BseGI GGATG 2 cut(s) 285, 783
BseLI CCNNNNNNNGG 5 cut(s) 346, 628, 642, 748, 896
BseMI GCAATG 1 cut(s) 432
BseMII CTCAG 3 cut(s) 264, 336, 458
BseNI ACTGG 3 cut(s) 111, 129, 328
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 5 cut(s) 365, 544, 556, 559, 715
BsgI GTGCAG 3 cut(s) 647, 707, 722
Bsh1285I CGRYCG 2 cut(s) 491, 759
BshFI GGCC 3 cut(s) 128, 764, 788
BshNI GGYRCC 1 cut(s) 618
BsiEI CGRYCG 2 cut(s) 491, 759
BsiHKAI GWGCWC 1 cut(s) 213
BsiHKCI CYCGRG 1 cut(s) 360
BsiSI CCGG 4 cut(s) 97, 346, 398, 623
BslFI GGGAC 3 cut(s) 113, 265, 362
BslI CCNNNNNNNGG 5 cut(s) 346, 628, 642, 748, 896
BsmAI GTCTC 2 cut(s) 239, 357
BsmBI CGTCTC 1 cut(s) 357
BsmFI GGGAC 3 cut(s) 113, 265, 362
BsnI GGCC 3 cut(s) 128, 764, 788
BsoBI CYCGRG 1 cut(s) 360
Bsp1286I GDGCHC 2 cut(s) 213, 673
Bsp143I GATC 5 cut(s) 201, 457, 488, 756, 848
BspACI CCGC 2 cut(s) 356, 455
BspANI GGCC 3 cut(s) 128, 764, 788
BspCNI CTCAG 3 cut(s) 265, 335, 457
BspLI GGNNCC 5 cut(s) 101, 102, 253, 254, 620
BspMAI CTGCAG 2 cut(s) 546, 561
BspMI ACCTGC 1 cut(s) 109
BspPI GGATC 1 cut(s) 465
BspQI GCTCTTC 1 cut(s) 98
BspT107I GGYRCC 1 cut(s) 618
BsrDI GCAATG 1 cut(s) 432
BsrI ACTGG 3 cut(s) 111, 129, 328
BssECI CCNNGG 1 cut(s) 636
BssMI GATC 5 cut(s) 201, 457, 488, 756, 848
BssNI GRCGYC 2 cut(s) 366, 575
Bst4CI ACNGT 3 cut(s) 45, 464, 723
Bst6I CTCTTC 3 cut(s) 98, 270, 751
BstACI GRCGYC 2 cut(s) 366, 575
BstAPI GCANNNNNTGC 2 cut(s) 556, 700
BstBAI YACGTR 1 cut(s) 469
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 3 cut(s) 273, 322, 444
BstF5I GGATG 2 cut(s) 285, 783
BstKTI GATC 5 cut(s) 204, 460, 491, 759, 851
BstMAI GTCTC 2 cut(s) 239, 357
BstMBI GATC 5 cut(s) 201, 457, 488, 756, 848
BstMCI CGRYCG 2 cut(s) 491, 759
BstMWI GCNNNNNNNGC 5 cut(s) 187, 541, 556, 700, 712
BstSCI CCNGG 4 cut(s) 96, 345, 396, 621
BstSFI CTRYAG 2 cut(s) 542, 557
BstSNI TACGTA 1 cut(s) 469
BstV1I GCAGC 5 cut(s) 365, 544, 556, 559, 715
BstV2I GAAGAC 2 cut(s) 486, 730
BstXI CCANNNNNNTGG 1 cut(s) 870
BsuRI GGCC 3 cut(s) 128, 764, 788
BtsCI GGATG 2 cut(s) 285, 783
BtsIMutI CAGTG 2 cut(s) 104, 719
BveI ACCTGC 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 213
CaiI CAGNNNCTG 1 cut(s) 124
Cfr13I GGNCC 4 cut(s) 58, 100, 127, 252
CseI GACGC 2 cut(s) 359, 583
Csp6I GTAC 3 cut(s) 70, 122, 619
CviAII CATG 2 cut(s) 55, 115
CviQI GTAC 3 cut(s) 70, 122, 619
DdeI CTNAG 3 cut(s) 273, 322, 444
DpnI GATC 5 cut(s) 203, 459, 490, 758, 850
DpnII GATC 5 cut(s) 201, 457, 488, 756, 848
Eam1104I CTCTTC 3 cut(s) 98, 270, 751
EarI CTCTTC 3 cut(s) 98, 270, 751
Ecl136II GAGCTC 1 cut(s) 211
Eco105I TACGTA 1 cut(s) 469
Eco24I GRGCYC 2 cut(s) 213, 673
Eco47I GGWCC 3 cut(s) 58, 100, 252
Eco53kI GAGCTC 1 cut(s) 211
Eco88I CYCGRG 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 211
EcoO109I RGGNCCY 2 cut(s) 100, 252
EcoT38I GRGCYC 2 cut(s) 213, 673
Esp3I CGTCTC 1 cut(s) 357
FaeI CATG 2 cut(s) 58, 118
FaqI GGGAC 3 cut(s) 113, 265, 362
FatI CATG 2 cut(s) 54, 114
FbaI TGATCA 1 cut(s) 201
Fnu4HI GCNGC 6 cut(s) 354, 357, 533, 545, 548, 704
FokI GGATG 2 cut(s) 272, 790
FriOI GRGCYC 2 cut(s) 213, 673
Fsp4HI GCNGC 6 cut(s) 354, 357, 533, 545, 548, 704
GluI GCNGC 6 cut(s) 354, 357, 533, 545, 548, 704
GsuI CTGGAG 2 cut(s) 128, 164
HaeIII GGCC 3 cut(s) 128, 764, 788
HapII CCGG 4 cut(s) 97, 346, 398, 623
HgaI GACGC 2 cut(s) 359, 583
Hin1I GRCGYC 2 cut(s) 366, 575
Hin1II CATG 2 cut(s) 58, 118
HincII GTYRAC 1 cut(s) 382
HindII GTYRAC 1 cut(s) 382
HinfI GANTC 6 cut(s) 110, 221, 437, 632, 675, 680
HpaII CCGG 4 cut(s) 97, 346, 398, 623
HphI GGTGA 4 cut(s) 181, 329, 652, 832
Hpy166II GTNNAC 2 cut(s) 382, 795
Hpy188I TCNGA 4 cut(s) 248, 274, 447, 637
Hpy188III TCNNGA 3 cut(s) 160, 236, 362
Hpy8I GTNNAC 2 cut(s) 382, 795
Hpy99I CGWCG 3 cut(s) 410, 577, 757
HpyAV CCTTC 4 cut(s) 307, 415, 489, 834
HpyCH4III ACNGT 3 cut(s) 45, 464, 723
HpyCH4IV ACGT 5 cut(s) 165, 366, 468, 605, 813
HpyCH4V TGCA 9 cut(s) 118, 418, 430, 544, 559, 628, 688, 694, 703
HpyF10VI GCNNNNNNNGC 5 cut(s) 187, 541, 556, 700, 712
HpyF3I CTNAG 3 cut(s) 273, 322, 444
HpySE526I ACGT 5 cut(s) 165, 366, 468, 605, 813
Hsp92I GRCGYC 2 cut(s) 366, 575
Hsp92II CATG 2 cut(s) 58, 118
KflI GGGWCCC 2 cut(s) 100, 252
KpnI GGTACC 1 cut(s) 622
Ksp22I TGATCA 1 cut(s) 201
Kzo9I GATC 5 cut(s) 201, 457, 488, 756, 848
LguI GCTCTTC 1 cut(s) 98
LmnI GCTCC 1 cut(s) 892
Lsp1109I GCAGC 5 cut(s) 365, 544, 556, 559, 715
LweI GCATC 2 cut(s) 168, 681
MaeII ACGT 5 cut(s) 165, 366, 468, 605, 813
MaeIII GTNAC 4 cut(s) 367, 464, 474, 601
MalI GATC 5 cut(s) 203, 459, 490, 758, 850
MboI GATC 5 cut(s) 201, 457, 488, 756, 848
MboII GAAGA 5 cut(s) 85, 257, 486, 730, 738
MfeI CAATTG 1 cut(s) 882
MhlI GDGCHC 2 cut(s) 213, 673
MluCI AATT 4 cut(s) 75, 522, 732, 882
MlyI GAGTC 4 cut(s) 119, 230, 641, 689
MseI TTAA 1 cut(s) 525
MspA1I CMGCKG 1 cut(s) 356
MspI CCGG 4 cut(s) 97, 346, 398, 623
MspR9I CCNGG 4 cut(s) 98, 347, 398, 623
MunI CAATTG 1 cut(s) 882
MwoI GCNNNNNNNGC 5 cut(s) 187, 541, 556, 700, 712
NciI CCSGG 4 cut(s) 98, 347, 398, 623
NdeII GATC 5 cut(s) 201, 457, 488, 756, 848
NlaIII CATG 2 cut(s) 58, 118
NlaIV GGNNCC 5 cut(s) 101, 102, 253, 254, 620
NmeAIII GCCGAG 1 cut(s) 861
NmuCI GTSAC 2 cut(s) 367, 601
PaeR7I CTCGAG 1 cut(s) 360
PciSI GCTCTTC 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 381
PfeI GAWTC 2 cut(s) 437, 675
PfoI TCCNGGA 1 cut(s) 96
PkrI GCNGC 6 cut(s) 355, 358, 534, 546, 549, 705
Ple19I CGATCG 2 cut(s) 491, 759
PleI GAGTC 4 cut(s) 118, 229, 640, 688
PpsI GAGTC 4 cut(s) 118, 229, 640, 688
Ppu21I YACGTR 1 cut(s) 469
PpuMI RGGWCCY 2 cut(s) 100, 252
PshBI ATTAAT 1 cut(s) 525
Psp124BI GAGCTC 1 cut(s) 213
Psp5II RGGWCCY 2 cut(s) 100, 252
PspN4I GGNNCC 5 cut(s) 101, 102, 253, 254, 620
PspPI GGNCC 4 cut(s) 58, 100, 127, 252
PspPPI RGGWCCY 2 cut(s) 100, 252
PstI CTGCAG 2 cut(s) 546, 561
PstNI CAGNNNCTG 1 cut(s) 124
PvuI CGATCG 2 cut(s) 491, 759
RsaI GTAC 3 cut(s) 71, 123, 620
RsaNI GTAC 3 cut(s) 70, 122, 619
SacI GAGCTC 1 cut(s) 213
SapI GCTCTTC 1 cut(s) 98
SaqAI TTAA 1 cut(s) 525
SatI GCNGC 6 cut(s) 354, 357, 533, 545, 548, 704
Sau3AI GATC 5 cut(s) 201, 457, 488, 756, 848
Sau96I GGNCC 4 cut(s) 58, 100, 127, 252
SchI GAGTC 4 cut(s) 119, 230, 641, 689
ScrFI CCNGG 4 cut(s) 98, 347, 398, 623
SduI GDGCHC 2 cut(s) 213, 673
SfaNI GCATC 2 cut(s) 168, 681
SfcI CTRYAG 2 cut(s) 542, 557
Sfr274I CTCGAG 1 cut(s) 360
SinI GGWCC 3 cut(s) 58, 100, 252
SlaI CTCGAG 1 cut(s) 360
SmlI CTYRAG 1 cut(s) 360
SmoI CTYRAG 1 cut(s) 360
SnaBI TACGTA 1 cut(s) 469
Sse9I AATT 4 cut(s) 75, 522, 732, 882
SsiI CCGC 2 cut(s) 356, 455
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 4 cut(s) 96, 345, 396, 621
TaaI ACNGT 3 cut(s) 45, 464, 723
TaiI ACGT 5 cut(s) 168, 369, 471, 608, 816
TaqI TCGA 7 cut(s) 8, 237, 361, 405, 673, 678, 759
TaqII GACCGA 1 cut(s) 75
TasI AATT 4 cut(s) 75, 522, 732, 882
TatI WGTACW 1 cut(s) 69
TauI GCSGC 1 cut(s) 359
TfiI GAWTC 2 cut(s) 437, 675
Tru1I TTAA 1 cut(s) 525
Tru9I TTAA 1 cut(s) 525
TscAI CASTG 2 cut(s) 111, 726
TseFI GTSAC 2 cut(s) 367, 601
TseI GCWGC 5 cut(s) 353, 532, 544, 547, 703
Tsp45I GTSAC 2 cut(s) 367, 601
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 2 cut(s) 111, 726
VpaK11BI GGWCC 3 cut(s) 58, 100, 252
VspI ATTAAT 1 cut(s) 525
XcmI CCANNNNNNNNNTGG 2 cut(s) 121, 867
XhoI CTCGAG 1 cut(s) 360
ZraI GACGTC 1 cut(s) 367
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.